ANRIL, an antisense noncoding RNA, has recently been identified at 9p21.3 (Pasmant et al.,2007). The first exon of ANRIL is located in the CDKN2A P14ARF promoter, and the gene overlaps CDKN2B. There is evidence that ANRIL may influence transcription of the CDKN2A and CDKN2B genes (Jarinova et al.,2009; Liu et al.,2009). The frequency of deletion of ANRIL in primary melanoma tumors could not be investigated in this study as the MLPA assay used does not include probes specific for the ANRIL gene.