To further evaluate functionality of neurogenesis after ischemia we examined dorso-ventral distribution of the new neuron density. It has been previously reported that adult neurogenesis in young animals is most pronounced in the dorsal (or septal) hippocampal region as opposed to the ventral (or caudal) region (Snyder et al., 2008). In older animals this gradient is diminished (Snyder et al., 2009b) and there is a corresponding shift in responsiveness of new neurons to physiological stimuli, such as running, toward the ventral region (Snyder et al., 2009b). Our results confirm that there was no dorso-ventral gradient in the density of DCX+ cells in control, aging animals. The numbers of DCX+ young neurons in the dorsal third and ventral third of the hippocampus on day 35 were 7.6 ± 3.3(SD)/section and 8.6 ± 2.2(SD)/section, respectively (P = 0.61, t-test, N.S.). These values were increased to 26.6 ± 3(SD)/section and 13.7 ± 4.4(SD)/section after ischemia (P = 0.014, t-test) thus restoring the twofold dorso-ventral difference that is normally seen in young animals.