To measure new cell production in DG we injected BrdU on day 7 after the ischemia and perfused animals on days 10, 35, and 90. Day 7 was chosen on the basis of past experiments showing the peak of post-ischemic cell production at that time point (Liu et al., 1998; Sharp et al., 2002). The three post-BrdU survival intervals allow for measurements of net cell proliferation, neuronal differentiation, maturation and long-term survival. On the post-ischemic days 10, 35, and 90 the numbers of BrdU+ cells were increased by 240%, 960% and 590%, respectively (Figure 2, Table 2).