Table 1 includes detailed measurements of NeuN+ cell layers in CA1 and DG at 10, 35, and 90 days, following the ischemia. Only the CA1 fields showed significant decrease in the stained areas suggesting loss of neurons. The neuronal loss, as measured by NeuN staining, was almost complete (99%) in the dorsal CA1 and less severe (20–50%) in the ventral region. All these results confirm that 2VO procedure in 1-year-old animals had effects on neuronal survival and death that were similar to previously observed effects in young (3-months old) adults (Kee et al., 2001; Wang et al., 2005).