Table 1.  Summary of NeuN+ area measurements indicating regional changes in hippocampal cell layers at different time points after ischemia.
10 days  35 days  90 days
Area  Sham  2VO  %Δ  Sham  2VO  %Δ  Sham  2VO  %Δ
DG Dorsal  230,000 ± 19,920  214,100 ± 4,500  −7%  213,200 ± 30,410  255,600 ± 29,830  ±20%  230,800 ± 24,240  288,800* ± 50,390  ±25%
DG Ventral  193,100 ± 9,840  191,100 ± 27,360  −1.4%  178,800 ± 12,290  200,300 ± 11,870  −12%  200,800 ± 19,320  202,700 ± 7,410  ±1%
CM Dorsal  131,200 ± 20,160  8,200* ± 725  −94%  124,500 ± 9,410  1,500* ± 1,120  −99%  124,700 ± 7,240  8,900* ± 12,950  −93%
CM Ventral  56,900 ± 7,640  45,600* ± 13,790  −20%  83,700 ± 12,760  38,600* ± 5,340  −54%  68,900 ± 6,570  39,800* ± 26,900  −42%
Mean areas (μm2) and standard deviations are shown for each group. *indicates statistically significant difference between sham and 2VO groups. 2 way ANOVA with n = 3 in each group taking total time and treatment as variables. Area measurements (μm2) were done using Image J software as detailed in methods.