Anti-SeMac antisera
Four female outbred CD-1 Swiss mice (4-week-old) (Charles River Laboratories) were immunized subcutaneously with 50 µg of recombinant SeMac suspended in 200 µl of saline emulsified in 44 µl of monophosphoryl lipid A-synthetic trehalose dicorynomycolate adjuvant (Corixa, Hamilton, Mont.). The animals were anesthetized by isoflurane inhalation prior to immunization and blood collection. Blood was collected prior to active immunization to prepare control sera. Mice were boosted at weeks 2 and 4 with 50 µg of protein mixed with the adjuvant. Anti-SeMac antiserum was prepared from blood obtained 1 week after the second booster.