Variable Material Bleaching agents Mean ΔE SD Regression analysis for materials Regression analysis for bleach agents After first bleach Durafil VS Beyond 0.66 0.22 0.496 0.519 Luma 0.82 0.17 Opal XB 0.73 0.14 TPH3 Beyond 0.6 0.16 Luma 0.54 0.26 Opal XB 0.84 0.36 After staining Durafil VS Beyond (–)4.44 1.52 0.001* 0.587 Luma (–)3.43 0.55 Opal XB (–)4.06 1.49 TPH3 Beyond (–)2.12 0.33 Luma (–)2.02 0.34 Opal XB (–)1.68 0.49 After second bleach Durafil VS Beyond 0.91 0.21 0.035* 0.114 Luma 1.55 0.52 Opal XB 3.71 1.8 TPH3 Beyond 1.31 0.49 Luma 1.49 0.35 Opal XB 0.64 0.26 Between staining and second bleach Beyond 4.16 1.09 <0.001* 0.002*