Variable Material Bleaching agents Mean ΔE SD Regression analysis for materials Regression analysis for bleach agents After first bleach Durafil VS Beyond 1.97 0.45 0.976 0.009* Luma 1.07 0.47 Opal XB 0.56 0.014 TPH3 Beyond 1.28 0.056 Luma 0.84 0.12 Opal XB 1.47 0.3 After staining Durafil VS Beyond 2.95(–) 0.29 0.15 0.025* Luma 3.40(–) 0.24 Opal XB 2.02(–) 0.73 TPH3 Beyond 2.04(–) 0.36 Luma 2.82(–) 0.18 Opal XB 2.54(–) 0.13 After second bleach Durafil VS Beyond 2.02 0.58 0.302 0.986 Luma 1.78 0.03 Opal XB 1.38 0.79 TPH3 Beyond 1.14 0.65 Luma 1.43 0.27 Opal XB 1.72 0.02 Between staining and second bleach Durafil VS Beyond 3.11 0.98 0.036* 0.007* Luma 2.73 0.47 Opal XB 0.93 0.24 TPH3 Beyond 1.64 0.23 Luma 1.8 0.26 Opal XB 1.42 0.05