ti wavelength fluorescence Fluorescence detection for AUC offers exquisite sensitivity and unparalleled solute discrimination for characterizing high-affinity interactions (Kingsbury et al. 2008) and the association state of proteins in serum and cell lysates (Kingsbury et al. 2008; Kroe and Laue 2009). The XLI AU-FDS fluorescence system is constrained to a single excitation wavelength and long-pass emission filter (MacGregor et al. 2004). A fluorescence system will be built that provides multiple excitation sources and four emission filters. This system will allow fluorescence and fluorescence resonance energy transfer characterization by AUC of multiple components and their complexes in biological fluids (e.g. serum, sputum, and cell lysates). Applications for these capabilities include detection of antigens and antibodies in biological fluids for medical research, and the direct physical characterization of complexes in proteomics research (Kroe and Laue 2009).
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