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PMC:2806624 / 53017-54553 JSONTXT

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pmc-enju-pas

Id Subject Object Predicate Lexical cue
T25850 0-8 NN denotes Promoter
T25851 9-15 NN denotes enzyme
T25852 16-22 NN denotes immuno
T25853 23-28 NN denotes assay
T25854 30-32 IN denotes As
T25855 33-42 VB denotes performed
T25856 43-45 IN denotes in
T25857 46-49 DT denotes the
T25858 50-59 JJ denotes pull-down
T25859 60-65 NN denotes assay
T25860 65-66 -COMMA- denotes ,
T25861 67-74 NN denotes HEK293T
T25862 75-80 NN denotes cells
T25863 81-85 VB denotes were
T25864 86-97 VB denotes transfected
T25865 98-102 IN denotes with
T25866 103-108 NN denotes RUNX1
T25867 109-111 CC denotes or
T25868 112-117 NN denotes RUNX3
T25869 118-121 CC denotes and
T25870 122-134 RB denotes subsequently
T25871 135-140 VB denotes lysed
T25872 142-151 JJ denotes Insoluble
T25873 152-160 NN denotes material
T25874 161-164 VB denotes was
T25875 165-172 VB denotes removed
T25876 173-175 IN denotes by
T25877 176-190 NN denotes centrifugation
T25878 192-200 JJ denotes 384-well
T25879 201-207 NN denotes plates
T25880 207-208 -COMMA- denotes ,
T25881 209-218 VB denotes precoated
T25882 219-223 IN denotes with
T25883 224-236 NN denotes streptavidin
T25884 237-238 -LRB- denotes (
T25885 238-244 NNP denotes Thermo
T25886 245-251 NNP denotes Fisher
T25887 252-262 NNP denotes Scientific
T25888 262-263 -RRB- denotes )
T25889 264-268 VB denotes were
T25890 269-275 VB denotes washed
T25891 276-277 CD denotes 3
T25892 278-283 NN denotes times
T25893 284-288 IN denotes with
T25894 289-296 VB denotes washing
T25895 297-303 NN denotes buffer
T25896 304-305 -LRB- denotes (
T25897 305-308 NNP denotes PBS
T25898 309-312 CC denotes and
T25899 313-317 CD denotes 0.05
T25900 317-318 NN denotes %
T25901 319-324 CD denotes Tween
T25902 325-327 CD denotes 20
T25903 327-328 -RRB- denotes )
T25904 330-342 VB denotes Biotinylated
T25905 343-348 NN denotes FOXP3
T25906 349-374 NN denotes promoter/oligonucleotides
T25907 375-381 NN denotes probes
T25908 382-392 VB denotes containing
T25909 393-396 DT denotes the
T25910 397-401 NN denotes RUNX
T25911 402-409 NN denotes binding
T25912 410-415 NN denotes sites
T25913 416-420 VB denotes were
T25914 421-426 VB denotes added
T25915 427-428 -LRB- denotes (
T25916 428-429 CD denotes 1
T25917 430-434 NN denotes pmol
T25918 435-438 IN denotes per
T25919 439-443 RB denotes well
T25920 443-444 -COLON- denotes ;
T25921 445-447 CD denotes 50
T25922 448-455 NN denotes fmol/µl
T25923 455-456 -RRB- denotes )
T25924 457-460 CC denotes and
T25925 461-470 VB denotes incubated
T25926 471-474 IN denotes for
T25927 475-476 CD denotes 1
T25928 477-478 NN denotes h
T25929 479-481 IN denotes at
T25930 482-486 NN denotes room
T25931 487-498 NN denotes temperature
T25932 500-506 CC denotes Either
T25933 507-508 DT denotes a
T25934 509-520 NN denotes combination
T25935 521-523 IN denotes of
T25936 524-527 DT denotes all
T25937 528-533 CD denotes three
T25938 534-550 NN denotes oligonucleotides
T25939 551-561 VB denotes containing
T25940 562-565 DT denotes the
T25941 566-573 VB denotes mutated
T25942 574-576 CC denotes or
T25943 577-580 DT denotes the
T25944 581-590 JJ denotes wild-type
T25945 591-598 NN denotes binding
T25946 599-604 NN denotes sites
T25947 605-608 VB denotes was
T25948 609-613 VB denotes used
T25949 614-616 CC denotes or
T25950 617-623 JJ denotes single
T25951 624-640 NN denotes oligonucleotides
T25952 641-645 VB denotes were
T25953 646-650 VB denotes used
T25954 651-653 IN denotes in
T25955 654-657 DT denotes the
T25956 658-663 NN denotes assay
T25957 665-670 IN denotes After
T25958 671-672 CD denotes 3
T25959 673-680 NN denotes washing
T25960 681-686 NN denotes steps
T25961 687-691 IN denotes with
T25962 692-699 VB denotes washing
T25963 700-706 NN denotes buffer
T25964 706-707 -COMMA- denotes ,
T25965 708-711 DT denotes the
T25966 712-719 JJ denotes nuclear
T25967 720-727 NN denotes extract
T25968 728-731 VB denotes was
T25969 732-737 VB denotes added
T25970 738-739 -LRB- denotes (
T25971 739-752 NN denotes concentration
T25972 753-754 SYM denotes >
T25973 755-758 CD denotes 0.2
T25974 759-764 NN denotes µg/µl
T25975 764-765 -RRB- denotes )
T25976 766-769 CC denotes and
T25977 770-779 VB denotes incubated
T25978 780-789 RB denotes overnight
T25979 790-792 IN denotes at
T25980 793-797 NNP denotes 4°C.
T25981 798-801 DT denotes The
T25982 802-809 NN denotes lysates
T25983 810-814 VB denotes were
T25984 815-824 VB denotes incubated
T25985 825-829 IN denotes with
T25986 830-832 CD denotes 10
T25987 833-835 NN denotes µg
T25988 836-838 IN denotes of
T25989 839-872 JJ denotes poly-deoxyinosinic-deoxycytidylic
T25990 873-877 NN denotes acid
T25991 878-879 -LRB- denotes (
T25992 879-892 JJ denotes Sigma-Aldrich
T25993 892-893 -RRB- denotes )
T25994 895-898 DT denotes The
T25995 899-904 NN denotes plate
T25996 905-908 VB denotes was
T25997 909-915 VB denotes washed
T25998 916-920 IN denotes with
T25999 921-925 NN denotes HKMG
T26000 926-932 NN denotes buffer
T26001 933-936 CC denotes and
T26002 937-946 VB denotes incubated
T26003 947-951 IN denotes with
T26004 952-953 DT denotes a
T26005 954-955 CD denotes 1
T26006 955-956 -COLON- denotes :
T26007 956-960 CD denotes 1000
T26008 961-969 NN denotes dilution
T26009 970-972 IN denotes of
T26010 973-979 NN denotes rabbit
T26011 980-990 NN denotes anti-RUNX1
T26012 991-992 -LRB- denotes (
T26013 992-999 NN denotes ab11903
T26014 999-1000 -COMMA- denotes ,
T26015 1001-1006 NN denotes Abcam
T26016 1006-1007 -RRB- denotes )
T26017 1008-1010 CC denotes or
T26018 1011-1012 CD denotes 1
T26019 1012-1013 -COLON- denotes :
T26020 1013-1016 CD denotes 200
T26021 1017-1025 NN denotes dilution
T26022 1026-1028 IN denotes of
T26023 1029-1035 NN denotes rabbit
T26024 1036-1046 NN denotes anti-RUNX3
T26025 1047-1048 -LRB- denotes (
T26026 1048-1052 NN denotes H-50
T26027 1052-1053 -COMMA- denotes ,
T26028 1054-1059 NNP denotes Santa
T26029 1060-1064 NNP denotes Cruz
T26030 1065-1078 NNP denotes Biotechnology
T26031 1078-1079 -COMMA- denotes ,
T26032 1080-1084 NNP denotes Inc.
T26033 1084-1085 -RRB- denotes )
T26034 1086-1088 IN denotes at
T26035 1089-1092 NN denotes 4°C
T26036 1093-1096 IN denotes for
T26037 1097-1098 CD denotes 2
T26038 1099-1100 NN denotes h
T26039 1102-1107 IN denotes After
T26040 1108-1113 CD denotes three
T26041 1114-1121 VB denotes washing
T26042 1122-1127 NN denotes steps
T26043 1128-1132 IN denotes with
T26044 1133-1137 NN denotes HKMG
T26045 1138-1144 NN denotes buffer
T26046 1144-1145 -COMMA- denotes ,
T26047 1146-1147 DT denotes a
T26048 1148-1157 JJ denotes secondary
T26049 1158-1166 NN denotes antibody
T26050 1167-1168 -LRB- denotes (
T26051 1168-1179 NN denotes anti–rabbit
T26052 1180-1187 NN denotes IgG-HRP
T26053 1187-1188 -COMMA- denotes ,
T26054 1189-1190 CD denotes 1
T26055 1190-1191 -COLON- denotes :
T26056 1191-1196 CD denotes 3,000
T26057 1197-1199 IN denotes in
T26058 1200-1204 NN denotes HKMG
T26059 1205-1211 NN denotes buffer
T26060 1211-1212 -COMMA- denotes ,
T26061 1213-1217 NNP denotes Cell
T26062 1218-1227 NNP denotes Signaling
T26063 1228-1238 NNP denotes Technology
T26064 1238-1239 -RRB- denotes )
T26065 1240-1243 VB denotes was
T26066 1244-1249 VB denotes added
T26067 1249-1250 -COMMA- denotes ,
T26068 1251-1254 CC denotes and
T26069 1255-1258 DT denotes the
T26070 1259-1264 NN denotes plate
T26071 1265-1268 VB denotes was
T26072 1269-1278 VB denotes incubated
T26073 1279-1282 IN denotes for
T26074 1283-1284 CD denotes 1
T26075 1285-1286 NN denotes h
T26076 1287-1289 IN denotes at
T26077 1290-1294 NNP denotes 4°C.
T26078 1295-1298 DT denotes The
T26079 1299-1304 NN denotes wells
T26080 1305-1309 VB denotes were
T26081 1310-1316 VB denotes washed
T26082 1317-1318 CD denotes 4
T26083 1319-1324 NN denotes times
T26084 1325-1329 IN denotes with
T26085 1330-1334 NN denotes HKMG
T26086 1335-1341 NN denotes buffer
T26087 1342-1348 IN denotes before
T26088 1349-1355 VB denotes adding
T26089 1356-1359 DT denotes the
T26090 1360-1369 NN denotes substrate
T26091 1370-1377 NN denotes reagent
T26092 1378-1379 -LRB- denotes (
T26093 1379-1380 NN denotes R
T26094 1380-1381 CC denotes &
T26095 1381-1382 NNP denotes D
T26096 1383-1390 NNP denotes Systems
T26097 1390-1391 -RRB- denotes )
T26098 1393-1396 DT denotes The
T26099 1397-1409 JJ denotes colorimetric
T26100 1410-1418 NN denotes reaction
T26101 1419-1422 VB denotes was
T26102 1423-1430 VB denotes stopped
T26103 1431-1433 IN denotes by
T26104 1434-1440 VB denotes adding
T26105 1441-1442 CD denotes 2
T26106 1443-1444 NN denotes M
T26107 1445-1450 NN denotes H2SO4
T26108 1452-1462 NN denotes Absorbance
T26109 1463-1465 IN denotes at
T26110 1466-1469 CD denotes 450
T26111 1470-1472 NN denotes nm
T26112 1473-1476 VB denotes was
T26113 1477-1485 VB denotes measured
T26114 1486-1491 VB denotes using
T26115 1492-1493 DT denotes a
T26116 1494-1504 NN denotes microplate
T26117 1505-1511 NN denotes reader
T26118 1512-1513 -LRB- denotes (
T26119 1513-1521 NNP denotes Berthold
T26120 1522-1534 NNP denotes Technologies
T26121 1534-1535 -RRB- denotes )
R20134 T25853 T25850 arg1Of assay,Promoter
R20135 T25853 T25851 arg1Of assay,enzyme
R20136 T25853 T25852 arg1Of assay,immuno
R20137 T25855 T25854 arg2Of performed,As
R20138 T25855 T25856 arg1Of performed,in
R20139 T25859 T25856 arg2Of assay,in
R20140 T25859 T25857 arg1Of assay,the
R20141 T25859 T25858 arg1Of assay,pull-down
R20142 T25862 T25861 arg1Of cells,HEK293T
R20143 T25862 T25863 arg1Of cells,were
R20144 T25862 T25864 arg2Of cells,transfected
R20145 T25862 T25871 arg2Of cells,lysed
R20146 T25864 T25865 arg1Of transfected,with
R20147 T25864 T25869 arg1Of transfected,and
R20148 T25866 T25867 arg1Of RUNX1,or
R20149 T25867 T25865 arg2Of or,with
R20150 T25868 T25867 arg2Of RUNX3,or
R20151 T25869 T25854 arg1Of and,As
R20152 T25869 T25860 arg1Of and,","
R20153 T25869 T25863 arg2Of and,were
R20154 T25871 T25869 arg2Of lysed,and
R20155 T25871 T25870 arg1Of lysed,subsequently
R20156 T25873 T25872 arg1Of material,Insoluble
R20157 T25873 T25874 arg1Of material,was
R20158 T25873 T25875 arg2Of material,removed
R20159 T25875 T25874 arg2Of removed,was
R20160 T25877 T25875 arg1Of centrifugation,removed
R20161 T25877 T25876 arg2Of centrifugation,by
R20162 T25879 T25878 arg1Of plates,384-well
R20163 T25879 T25880 arg1Of plates,","
R20164 T25879 T25881 arg2Of plates,precoated
R20165 T25879 T25889 arg1Of plates,were
R20166 T25879 T25890 arg2Of plates,washed
R20167 T25881 T25882 arg1Of precoated,with
R20168 T25883 T25882 arg2Of streptavidin,with
R20169 T25883 T25884 arg1Of streptavidin,(
R20170 T25887 T25884 arg2Of Scientific,(
R20171 T25887 T25885 arg1Of Scientific,Thermo
R20172 T25887 T25886 arg1Of Scientific,Fisher
R20173 T25888 T25884 arg3Of ),(
R20174 T25890 T25889 arg2Of washed,were
R20175 T25890 T25893 arg1Of washed,with
R20176 T25892 T25890 arg3Of times,washed
R20177 T25892 T25891 arg1Of times,3
R20178 T25894 T25893 arg2Of washing,with
R20179 T25895 T25894 arg2Of buffer,washing
R20180 T25895 T25896 arg1Of buffer,(
R20181 T25897 T25898 arg1Of PBS,and
R20182 T25898 T25896 arg2Of and,(
R20183 T25900 T25899 arg1Of %,0.05
R20184 T25902 T25898 arg2Of 20,and
R20185 T25902 T25900 arg1Of 20,%
R20186 T25902 T25901 arg1Of 20,Tween
R20187 T25903 T25896 arg3Of ),(
R20188 T25907 T25904 arg2Of probes,Biotinylated
R20189 T25907 T25905 arg1Of probes,FOXP3
R20190 T25907 T25906 arg1Of probes,promoter/oligonucleotides
R20191 T25907 T25908 arg1Of probes,containing
R20192 T25907 T25913 arg1Of probes,were
R20193 T25907 T25914 arg2Of probes,added
R20194 T25907 T25925 arg2Of probes,incubated
R20195 T25912 T25908 arg2Of sites,containing
R20196 T25912 T25909 arg1Of sites,the
R20197 T25912 T25910 arg1Of sites,RUNX
R20198 T25912 T25911 arg1Of sites,binding
R20199 T25914 T25915 arg1Of added,(
R20200 T25914 T25924 arg1Of added,and
R20201 T25917 T25916 arg1Of pmol,1
R20202 T25917 T25918 arg1Of pmol,per
R20203 T25917 T25920 arg1Of pmol,;
R20204 T25919 T25918 arg2Of well,per
R20205 T25920 T25915 arg2Of ;,(
R20206 T25922 T25920 arg2Of fmol/µl,;
R20207 T25922 T25921 arg1Of fmol/µl,50
R20208 T25923 T25915 arg3Of ),(
R20209 T25924 T25913 arg2Of and,were
R20210 T25925 T25924 arg2Of incubated,and
R20211 T25925 T25926 arg1Of incubated,for
R20212 T25925 T25929 arg1Of incubated,at
R20213 T25928 T25926 arg2Of h,for
R20214 T25928 T25927 arg1Of h,1
R20215 T25931 T25929 arg2Of temperature,at
R20216 T25931 T25930 arg1Of temperature,room
R20217 T25934 T25933 arg1Of combination,a
R20218 T25934 T25935 arg1Of combination,of
R20219 T25934 T25947 arg1Of combination,was
R20220 T25934 T25948 arg2Of combination,used
R20221 T25938 T25935 arg2Of oligonucleotides,of
R20222 T25938 T25936 arg1Of oligonucleotides,all
R20223 T25938 T25937 arg1Of oligonucleotides,three
R20224 T25938 T25939 arg1Of oligonucleotides,containing
R20225 T25941 T25942 arg1Of mutated,or
R20226 T25944 T25942 arg2Of wild-type,or
R20227 T25944 T25943 arg1Of wild-type,the
R20228 T25946 T25939 arg2Of sites,containing
R20229 T25946 T25940 arg1Of sites,the
R20230 T25946 T25941 arg1Of sites,mutated
R20231 T25946 T25944 arg1Of sites,wild-type
R20232 T25946 T25945 arg1Of sites,binding
R20233 T25948 T25947 arg2Of used,was
R20234 T25948 T25949 arg1Of used,or
R20235 T25949 T25932 arg1Of or,Either
R20236 T25951 T25950 arg1Of oligonucleotides,single
R20237 T25951 T25952 arg1Of oligonucleotides,were
R20238 T25951 T25953 arg2Of oligonucleotides,used
R20239 T25953 T25949 arg2Of used,or
R20240 T25953 T25952 arg2Of used,were
R20241 T25953 T25954 arg1Of used,in
R20242 T25956 T25954 arg2Of assay,in
R20243 T25956 T25955 arg1Of assay,the
R20244 T25960 T25957 arg2Of steps,After
R20245 T25960 T25958 arg1Of steps,3
R20246 T25960 T25959 arg1Of steps,washing
R20247 T25960 T25961 arg1Of steps,with
R20248 T25963 T25961 arg2Of buffer,with
R20249 T25963 T25962 arg1Of buffer,washing
R20250 T25967 T25965 arg1Of extract,the
R20251 T25967 T25966 arg1Of extract,nuclear
R20252 T25967 T25968 arg1Of extract,was
R20253 T25967 T25969 arg2Of extract,added
R20254 T25967 T25977 arg2Of extract,incubated
R20255 T25969 T25970 arg1Of added,(
R20256 T25969 T25976 arg1Of added,and
R20257 T25973 T25972 arg1Of 0.2,>
R20258 T25974 T25970 arg2Of µg/µl,(
R20259 T25974 T25971 arg1Of µg/µl,concentration
R20260 T25974 T25973 arg1Of µg/µl,0.2
R20261 T25975 T25970 arg3Of ),(
R20262 T25976 T25957 arg1Of and,After
R20263 T25976 T25964 arg1Of and,","
R20264 T25976 T25968 arg2Of and,was
R20265 T25977 T25976 arg2Of incubated,and
R20266 T25977 T25978 arg1Of incubated,overnight
R20267 T25977 T25979 arg1Of incubated,at
R20268 T25980 T25979 arg2Of 4°C.,at
R20269 T25980 T25983 modOf 4°C.,were
R20270 T25982 T25981 arg1Of lysates,The
R20271 T25982 T25983 arg1Of lysates,were
R20272 T25982 T25984 arg2Of lysates,incubated
R20273 T25984 T25983 arg2Of incubated,were
R20274 T25984 T25985 arg1Of incubated,with
R20275 T25987 T25985 arg2Of µg,with
R20276 T25987 T25986 arg1Of µg,10
R20277 T25987 T25988 arg1Of µg,of
R20278 T25990 T25988 arg2Of acid,of
R20279 T25990 T25989 arg1Of acid,poly-deoxyinosinic-deoxycytidylic
R20280 T25990 T25991 arg1Of acid,(
R20281 T25992 T25991 arg2Of Sigma-Aldrich,(
R20282 T25993 T25991 arg3Of ),(
R20283 T25995 T25994 arg1Of plate,The
R20284 T25995 T25996 arg1Of plate,was
R20285 T25995 T25997 arg2Of plate,washed
R20286 T25995 T26002 arg2Of plate,incubated
R20287 T25997 T25998 arg1Of washed,with
R20288 T25997 T26001 arg1Of washed,and
R20289 T26000 T25998 arg2Of buffer,with
R20290 T26000 T25999 arg1Of buffer,HKMG
R20291 T26001 T25996 arg2Of and,was
R20292 T26002 T26001 arg2Of incubated,and
R20293 T26002 T26003 arg1Of incubated,with
R20294 T26005 T26003 arg2Of 1,with
R20295 T26005 T26004 arg1Of 1,a
R20296 T26005 T26006 arg1Of 1,:
R20297 T26008 T26006 arg2Of dilution,:
R20298 T26008 T26007 arg1Of dilution,1000
R20299 T26008 T26009 arg1Of dilution,of
R20300 T26008 T26019 arg1Of dilution,:
R20301 T26011 T26010 arg1Of anti-RUNX1,rabbit
R20302 T26011 T26012 arg1Of anti-RUNX1,(
R20303 T26011 T26017 arg1Of anti-RUNX1,or
R20304 T26013 T26012 arg2Of ab11903,(
R20305 T26013 T26014 arg1Of ab11903,","
R20306 T26015 T26014 arg2Of Abcam,","
R20307 T26016 T26012 arg3Of ),(
R20308 T26017 T26009 arg2Of or,of
R20309 T26018 T26017 arg2Of 1,or
R20310 T26021 T26019 arg2Of dilution,:
R20311 T26021 T26020 arg1Of dilution,200
R20312 T26021 T26022 arg1Of dilution,of
R20313 T26021 T26025 arg1Of dilution,(
R20314 T26021 T26034 arg1Of dilution,at
R20315 T26024 T26022 arg2Of anti-RUNX3,of
R20316 T26024 T26023 arg1Of anti-RUNX3,rabbit
R20317 T26026 T26025 arg2Of H-50,(
R20318 T26026 T26027 arg1Of H-50,","
R20319 T26026 T26031 arg1Of H-50,","
R20320 T26030 T26027 arg2Of Biotechnology,","
R20321 T26030 T26028 arg1Of Biotechnology,Santa
R20322 T26030 T26029 arg1Of Biotechnology,Cruz
R20323 T26032 T26031 arg2Of Inc.,","
R20324 T26033 T26025 arg3Of ),(
R20325 T26035 T26034 arg2Of 4°C,at
R20326 T26035 T26036 arg1Of 4°C,for
R20327 T26038 T26036 arg2Of h,for
R20328 T26038 T26037 arg1Of h,2
R20329 T26042 T26039 arg2Of steps,After
R20330 T26042 T26040 arg1Of steps,three
R20331 T26042 T26041 arg1Of steps,washing
R20332 T26042 T26043 arg1Of steps,with
R20333 T26045 T26043 arg2Of buffer,with
R20334 T26045 T26044 arg1Of buffer,HKMG
R20335 T26049 T26047 arg1Of antibody,a
R20336 T26049 T26048 arg1Of antibody,secondary
R20337 T26049 T26050 arg1Of antibody,(
R20338 T26049 T26065 arg1Of antibody,was
R20339 T26049 T26066 arg2Of antibody,added
R20340 T26052 T26050 arg2Of IgG-HRP,(
R20341 T26052 T26051 arg1Of IgG-HRP,anti–rabbit
R20342 T26052 T26053 arg1Of IgG-HRP,","
R20343 T26052 T26055 arg1Of IgG-HRP,:
R20344 T26054 T26053 arg2Of 1,","
R20345 T26056 T26055 arg2Of "3,000",:
R20346 T26056 T26057 arg1Of "3,000",in
R20347 T26056 T26060 arg1Of "3,000",","
R20348 T26059 T26057 arg2Of buffer,in
R20349 T26059 T26058 arg1Of buffer,HKMG
R20350 T26063 T26060 arg2Of Technology,","
R20351 T26063 T26061 arg1Of Technology,Cell
R20352 T26063 T26062 arg1Of Technology,Signaling
R20353 T26064 T26050 arg3Of ),(
R20354 T26066 T26039 arg1Of added,After
R20355 T26066 T26046 arg1Of added,","
R20356 T26066 T26065 arg2Of added,was
R20357 T26066 T26068 arg1Of added,and
R20358 T26068 T26067 arg1Of and,","
R20359 T26070 T26069 arg1Of plate,the
R20360 T26070 T26071 arg1Of plate,was
R20361 T26070 T26072 arg2Of plate,incubated
R20362 T26072 T26071 arg2Of incubated,was
R20363 T26072 T26073 arg1Of incubated,for
R20364 T26072 T26076 arg1Of incubated,at
R20365 T26075 T26073 arg2Of h,for
R20366 T26075 T26074 arg1Of h,1
R20367 T26077 T26076 arg2Of 4°C.,at
R20368 T26079 T26078 arg1Of wells,The
R20369 T26079 T26080 arg1Of wells,were
R20370 T26079 T26081 arg2Of wells,washed
R20371 T26079 T26088 arg1Of wells,adding
R20372 T26081 T26068 arg2Of washed,and
R20373 T26081 T26071 modOf washed,was
R20374 T26081 T26080 arg2Of washed,were
R20375 T26081 T26087 arg1Of washed,before
R20376 T26083 T26081 arg3Of times,washed
R20377 T26083 T26082 arg1Of times,4
R20378 T26083 T26084 arg1Of times,with
R20379 T26086 T26084 arg2Of buffer,with
R20380 T26086 T26085 arg1Of buffer,HKMG
R20381 T26088 T26087 arg2Of adding,before
R20382 T26091 T26088 arg2Of reagent,adding
R20383 T26091 T26089 arg1Of reagent,the
R20384 T26091 T26090 arg1Of reagent,substrate
R20385 T26091 T26092 arg1Of reagent,(
R20386 T26096 T26092 arg2Of Systems,(
R20387 T26096 T26093 arg1Of Systems,R
R20388 T26096 T26094 arg1Of Systems,&
R20389 T26096 T26095 arg1Of Systems,D
R20390 T26097 T26092 arg3Of ),(
R20391 T26100 T26098 arg1Of reaction,The
R20392 T26100 T26099 arg1Of reaction,colorimetric
R20393 T26100 T26101 arg1Of reaction,was
R20394 T26100 T26102 arg2Of reaction,stopped
R20395 T26102 T26101 arg2Of stopped,was
R20396 T26102 T26103 arg1Of stopped,by
R20397 T26104 T26103 arg2Of adding,by
R20398 T26105 T26106 arg1Of 2,M
R20399 T26107 T26104 arg2Of H2SO4,adding
R20400 T26107 T26105 arg1Of H2SO4,2
R20401 T26108 T26109 arg1Of Absorbance,at
R20402 T26108 T26112 arg1Of Absorbance,was
R20403 T26108 T26113 arg2Of Absorbance,measured
R20404 T26111 T26109 arg2Of nm,at
R20405 T26111 T26110 arg1Of nm,450
R20406 T26113 T26112 arg2Of measured,was
R20407 T26114 T26113 arg3Of using,measured
R20408 T26117 T26114 arg2Of reader,using
R20409 T26117 T26115 arg1Of reader,a
R20410 T26117 T26116 arg1Of reader,microplate
R20411 T26117 T26118 arg1Of reader,(
R20412 T26120 T26118 arg2Of Technologies,(
R20413 T26120 T26119 arg1Of Technologies,Berthold
R20414 T26121 T26118 arg3Of ),(

bionlp-st-ge-2016-spacy-parsed

Id Subject Object Predicate Lexical cue
T26141 0-8 NN denotes Promoter
T26142 9-15 NN denotes enzyme
T26143 16-22 NN denotes immuno
T26144 23-28 NN denotes assay
T26145 28-29 . denotes .
T26146 30-32 IN denotes As
T26147 33-42 VBN denotes performed
T26148 43-45 IN denotes in
T26149 46-49 DT denotes the
T26150 50-59 JJ denotes pull-down
T26151 60-65 NN denotes assay
T26152 65-66 , denotes ,
T26153 67-74 CD denotes HEK293T
T26154 75-80 NNS denotes cells
T26155 81-85 VBD denotes were
T26156 86-97 VBN denotes transfected
T26157 98-102 IN denotes with
T26158 103-108 CD denotes RUNX1
T26159 109-111 CC denotes or
T26160 112-117 CD denotes RUNX3
T26161 118-121 CC denotes and
T26162 122-134 RB denotes subsequently
T26163 135-140 VBN denotes lysed
T26164 140-141 . denotes .
T26165 142-151 NNP denotes Insoluble
T26166 152-160 NN denotes material
T26167 161-164 VBD denotes was
T26168 165-172 VBN denotes removed
T26169 173-175 IN denotes by
T26170 176-190 NN denotes centrifugation
T26171 190-191 . denotes .
T26172 192-200 JJ denotes 384-well
T26173 201-207 NNS denotes plates
T26174 207-208 , denotes ,
T26175 209-218 VBN denotes precoated
T26176 219-223 IN denotes with
T26177 224-236 NNP denotes streptavidin
T26178 237-238 -LRB- denotes (
T26179 238-244 NNP denotes Thermo
T26180 245-251 NNP denotes Fisher
T26181 252-262 NNP denotes Scientific
T26182 262-263 -RRB- denotes )
T26183 264-268 VBD denotes were
T26184 269-275 VBN denotes washed
T26185 276-277 CD denotes 3
T26186 278-283 NNS denotes times
T26187 284-288 IN denotes with
T26188 289-296 VBG denotes washing
T26189 297-303 NN denotes buffer
T26190 304-305 -LRB- denotes (
T26191 305-308 NNP denotes PBS
T26192 309-312 CC denotes and
T26193 313-317 CD denotes 0.05
T26194 317-318 NN denotes %
T26195 319-324 CD denotes Tween
T26196 325-327 CD denotes 20
T26197 327-328 -RRB- denotes )
T26198 328-329 . denotes .
T26199 330-342 NNP denotes Biotinylated
T26200 343-348 NNP denotes FOXP3
T26201 349-374 VBZ denotes promoter/oligonucleotides
T26202 375-381 NNS denotes probes
T26203 382-392 VBG denotes containing
T26204 393-396 DT denotes the
T26205 397-401 NNP denotes RUNX
T26206 402-409 JJ denotes binding
T26207 410-415 NNS denotes sites
T26208 416-420 VBD denotes were
T26209 421-426 VBN denotes added
T26210 427-428 -LRB- denotes (
T26211 428-429 CD denotes 1
T26212 430-434 NN denotes pmol
T26213 435-438 IN denotes per
T26214 439-443 RB denotes well
T26215 443-444 : denotes ;
T26216 445-447 CD denotes 50
T26217 448-452 NN denotes fmol
T26218 452-453 NN denotes /
T26219 453-455 NN denotes µl
T26220 455-456 -RRB- denotes )
T26221 457-460 CC denotes and
T26222 461-470 VBN denotes incubated
T26223 471-474 IN denotes for
T26224 475-476 CD denotes 1
T26225 477-478 NN denotes h
T26226 479-481 IN denotes at
T26227 482-486 NN denotes room
T26228 487-498 NN denotes temperature
T26229 498-499 . denotes .
T26230 500-506 CC denotes Either
T26231 507-508 DT denotes a
T26232 509-520 NN denotes combination
T26233 521-523 IN denotes of
T26234 524-527 DT denotes all
T26235 528-533 CD denotes three
T26236 534-550 NNS denotes oligonucleotides
T26237 551-561 VBG denotes containing
T26238 562-565 DT denotes the
T26239 566-573 VBN denotes mutated
T26240 574-576 CC denotes or
T26241 577-580 DT denotes the
T26242 581-590 JJ denotes wild-type
T26243 591-598 JJ denotes binding
T26244 599-604 NNS denotes sites
T26245 605-608 VBD denotes was
T26246 609-613 VBN denotes used
T26247 614-616 CC denotes or
T26248 617-623 JJ denotes single
T26249 624-640 NNS denotes oligonucleotides
T26250 641-645 VBD denotes were
T26251 646-650 VBN denotes used
T26252 651-653 IN denotes in
T26253 654-657 DT denotes the
T26254 658-663 NN denotes assay
T26255 663-664 . denotes .
T26256 665-670 IN denotes After
T26257 671-672 CD denotes 3
T26258 673-680 VBG denotes washing
T26259 681-686 NNS denotes steps
T26260 687-691 IN denotes with
T26261 692-699 VBG denotes washing
T26262 700-706 NN denotes buffer
T26263 706-707 , denotes ,
T26264 708-711 DT denotes the
T26265 712-719 JJ denotes nuclear
T26266 720-727 VB denotes extract
T26267 728-731 VBD denotes was
T26268 732-737 VBN denotes added
T26269 738-739 -LRB- denotes (
T26270 739-752 NN denotes concentration
T26271 753-754 CD denotes >
T26272 755-758 CD denotes 0.2
T26273 759-761 NN denotes µg
T26274 761-762 NN denotes /
T26275 762-764 NN denotes µl
T26276 764-765 -RRB- denotes )
T26277 766-769 CC denotes and
T26278 770-779 JJ denotes incubated
T26279 780-789 JJ denotes overnight
T26280 790-792 IN denotes at
T26281 793-794 CD denotes 4
T26282 794-795 CD denotes °
T26283 795-796 NNP denotes C
T26284 796-797 . denotes .
T26285 798-801 DT denotes The
T26286 802-809 NNS denotes lysates
T26287 810-814 VBD denotes were
T26288 815-824 VBN denotes incubated
T26289 825-829 IN denotes with
T26290 830-832 CD denotes 10
T26291 833-835 NN denotes µg
T26292 836-838 IN denotes of
T26293 839-872 JJ denotes poly-deoxyinosinic-deoxycytidylic
T26294 873-877 NN denotes acid
T26295 878-879 -LRB- denotes (
T26296 879-892 NNP denotes Sigma-Aldrich
T26297 892-893 -RRB- denotes )
T26298 893-894 . denotes .
T26299 895-898 DT denotes The
T26300 899-904 NN denotes plate
T26301 905-908 VBD denotes was
T26302 909-915 VBN denotes washed
T26303 916-920 IN denotes with
T26304 921-925 NNP denotes HKMG
T26305 926-932 NN denotes buffer
T26306 933-936 CC denotes and
T26307 937-946 VBN denotes incubated
T26308 947-951 IN denotes with
T26309 952-953 DT denotes a
T26310 954-960 CD denotes 1:1000
T26311 961-969 NN denotes dilution
T26312 970-972 IN denotes of
T26313 973-979 NN denotes rabbit
T26314 980-990 NN denotes anti-RUNX1
T26315 991-992 -LRB- denotes (
T26316 992-999 CD denotes ab11903
T26317 999-1000 , denotes ,
T26318 1001-1006 NNP denotes Abcam
T26319 1006-1007 -RRB- denotes )
T26320 1008-1010 CC denotes or
T26321 1011-1016 CD denotes 1:200
T26322 1017-1025 NN denotes dilution
T26323 1026-1028 IN denotes of
T26324 1029-1035 NN denotes rabbit
T26325 1036-1046 NN denotes anti-RUNX3
T26326 1047-1048 -LRB- denotes (
T26327 1048-1052 NN denotes H-50
T26328 1052-1053 , denotes ,
T26329 1054-1059 NNP denotes Santa
T26330 1060-1064 NNP denotes Cruz
T26331 1065-1078 NNP denotes Biotechnology
T26332 1078-1079 , denotes ,
T26333 1080-1084 NNP denotes Inc.
T26334 1084-1085 -RRB- denotes )
T26335 1086-1088 IN denotes at
T26336 1089-1090 CD denotes 4
T26337 1090-1091 CD denotes °
T26338 1091-1092 NNP denotes C
T26339 1093-1096 IN denotes for
T26340 1097-1098 CD denotes 2
T26341 1099-1100 NN denotes h
T26342 1100-1101 . denotes .
T26343 1102-1107 IN denotes After
T26344 1108-1113 CD denotes three
T26345 1114-1121 VBG denotes washing
T26346 1122-1127 NNS denotes steps
T26347 1128-1132 IN denotes with
T26348 1133-1137 NNP denotes HKMG
T26349 1138-1144 NN denotes buffer
T26350 1144-1145 , denotes ,
T26351 1146-1147 DT denotes a
T26352 1148-1157 JJ denotes secondary
T26353 1158-1166 NN denotes antibody
T26354 1167-1168 -LRB- denotes (
T26355 1168-1172 NNS denotes anti
T26356 1173-1179 NN denotes rabbit
T26357 1180-1187 NNP denotes IgG-HRP
T26358 1187-1188 , denotes ,
T26359 1189-1196 CD denotes 1:3,000
T26360 1197-1199 IN denotes in
T26361 1200-1204 NNP denotes HKMG
T26362 1205-1211 NN denotes buffer
T26363 1211-1212 , denotes ,
T26364 1213-1217 NNP denotes Cell
T26365 1218-1227 NNP denotes Signaling
T26366 1228-1238 NNP denotes Technology
T26367 1238-1239 -RRB- denotes )
T26368 1240-1243 VBD denotes was
T26369 1244-1249 VBN denotes added
T26370 1249-1250 , denotes ,
T26371 1251-1254 CC denotes and
T26372 1255-1258 DT denotes the
T26373 1259-1264 NN denotes plate
T26374 1265-1268 VBD denotes was
T26375 1269-1278 VBN denotes incubated
T26376 1279-1282 IN denotes for
T26377 1283-1284 CD denotes 1
T26378 1285-1286 NN denotes h
T26379 1287-1289 IN denotes at
T26380 1290-1291 CD denotes 4
T26381 1291-1292 CD denotes °
T26382 1292-1293 NNP denotes C
T26383 1293-1294 . denotes .
T26384 1295-1298 DT denotes The
T26385 1299-1304 NNS denotes wells
T26386 1305-1309 VBD denotes were
T26387 1310-1316 VBN denotes washed
T26388 1317-1318 CD denotes 4
T26389 1319-1324 NNS denotes times
T26390 1325-1329 IN denotes with
T26391 1330-1334 NNP denotes HKMG
T26392 1335-1341 NN denotes buffer
T26393 1342-1348 IN denotes before
T26394 1349-1355 VBG denotes adding
T26395 1356-1359 DT denotes the
T26396 1360-1369 JJ denotes substrate
T26397 1370-1377 NN denotes reagent
T26398 1378-1379 -LRB- denotes (
T26399 1379-1382 NNP denotes R&D
T26400 1383-1390 NNPS denotes Systems
T26401 1390-1391 -RRB- denotes )
T26402 1391-1392 . denotes .
T26403 1393-1396 DT denotes The
T26404 1397-1409 JJ denotes colorimetric
T26405 1410-1418 NN denotes reaction
T26406 1419-1422 VBD denotes was
T26407 1423-1430 VBN denotes stopped
T26408 1431-1433 IN denotes by
T26409 1434-1440 VBG denotes adding
T26410 1441-1442 CD denotes 2
T26411 1443-1444 NNP denotes M
T26412 1445-1450 NNP denotes H2SO4
T26413 1450-1451 . denotes .
T26414 1452-1462 NNP denotes Absorbance
T26415 1463-1465 IN denotes at
T26416 1466-1469 CD denotes 450
T26417 1470-1472 NN denotes nm
T26418 1473-1476 VBD denotes was
T26419 1477-1485 VBN denotes measured
T26420 1486-1491 VBG denotes using
T26421 1492-1493 DT denotes a
T26422 1494-1504 NN denotes microplate
T26423 1505-1511 NN denotes reader
T26424 1512-1513 -LRB- denotes (
T26425 1513-1521 NNP denotes Berthold
T26426 1522-1534 NNPS denotes Technologies
T26427 1534-1535 -RRB- denotes )
T26428 1535-1536 . denotes .
R20415 T26141 T26141 ROOT Promoter,Promoter
R20416 T26142 T26144 compound enzyme,assay
R20417 T26143 T26144 compound immuno,assay
R20418 T26144 T26141 dobj assay,Promoter
R20419 T26145 T26141 punct .,Promoter
R20420 T26146 T26147 mark As,performed
R20421 T26147 T26156 advcl performed,transfected
R20422 T26148 T26147 prep in,performed
R20423 T26149 T26151 det the,assay
R20424 T26150 T26151 amod pull-down,assay
R20425 T26151 T26148 pobj assay,in
R20426 T26152 T26156 punct ",",transfected
R20427 T26153 T26154 nummod HEK293T,cells
R20428 T26154 T26156 nsubjpass cells,transfected
R20429 T26155 T26156 auxpass were,transfected
R20430 T26156 T26156 ROOT transfected,transfected
R20431 T26157 T26156 prep with,transfected
R20432 T26158 T26163 nummod RUNX1,lysed
R20433 T26159 T26158 cc or,RUNX1
R20434 T26160 T26158 conj RUNX3,RUNX1
R20435 T26161 T26160 cc and,RUNX3
R20436 T26162 T26160 conj subsequently,RUNX3
R20437 T26163 T26157 pobj lysed,with
R20438 T26164 T26156 punct .,transfected
R20439 T26165 T26166 amod Insoluble,material
R20440 T26166 T26168 nsubjpass material,removed
R20441 T26167 T26168 auxpass was,removed
R20442 T26168 T26168 ROOT removed,removed
R20443 T26169 T26168 agent by,removed
R20444 T26170 T26169 pobj centrifugation,by
R20445 T26171 T26168 punct .,removed
R20446 T26172 T26173 amod 384-well,plates
R20447 T26173 T26184 nsubjpass plates,washed
R20448 T26174 T26173 punct ",",plates
R20449 T26175 T26173 acl precoated,plates
R20450 T26176 T26175 prep with,precoated
R20451 T26177 T26176 pobj streptavidin,with
R20452 T26178 T26177 punct (,streptavidin
R20453 T26179 T26181 compound Thermo,Scientific
R20454 T26180 T26181 compound Fisher,Scientific
R20455 T26181 T26177 appos Scientific,streptavidin
R20456 T26182 T26177 punct ),streptavidin
R20457 T26183 T26184 auxpass were,washed
R20458 T26184 T26184 ROOT washed,washed
R20459 T26185 T26186 nummod 3,times
R20460 T26186 T26184 npadvmod times,washed
R20461 T26187 T26184 prep with,washed
R20462 T26188 T26191 meta washing,PBS
R20463 T26189 T26188 dobj buffer,washing
R20464 T26190 T26191 punct (,PBS
R20465 T26191 T26187 pobj PBS,with
R20466 T26192 T26191 cc and,PBS
R20467 T26193 T26194 nummod 0.05,%
R20468 T26194 T26196 nmod %,20
R20469 T26195 T26196 nummod Tween,20
R20470 T26196 T26191 conj 20,PBS
R20471 T26197 T26196 punct ),20
R20472 T26198 T26184 punct .,washed
R20473 T26199 T26200 compound Biotinylated,FOXP3
R20474 T26200 T26201 nsubj FOXP3,promoter/oligonucleotides
R20475 T26201 T26201 ROOT promoter/oligonucleotides,promoter/oligonucleotides
R20476 T26202 T26201 dobj probes,promoter/oligonucleotides
R20477 T26203 T26202 acl containing,probes
R20478 T26204 T26207 det the,sites
R20479 T26205 T26207 nmod RUNX,sites
R20480 T26206 T26207 amod binding,sites
R20481 T26207 T26203 dobj sites,containing
R20482 T26208 T26209 auxpass were,added
R20483 T26209 T26201 conj added,promoter/oligonucleotides
R20484 T26210 T26209 punct (,added
R20485 T26211 T26212 nummod 1,pmol
R20486 T26212 T26209 appos pmol,added
R20487 T26213 T26212 prep per,pmol
R20488 T26214 T26213 pobj well,per
R20489 T26215 T26212 punct ;,pmol
R20490 T26216 T26217 nummod 50,fmol
R20491 T26217 T26222 nsubj fmol,incubated
R20492 T26218 T26219 compound /,µl
R20493 T26219 T26217 appos µl,fmol
R20494 T26220 T26217 punct ),fmol
R20495 T26221 T26217 cc and,fmol
R20496 T26222 T26209 parataxis incubated,added
R20497 T26223 T26222 prep for,incubated
R20498 T26224 T26225 nummod 1,h
R20499 T26225 T26223 pobj h,for
R20500 T26226 T26225 prep at,h
R20501 T26227 T26228 compound room,temperature
R20502 T26228 T26226 pobj temperature,at
R20503 T26229 T26209 punct .,added
R20504 T26230 T26246 preconj Either,used
R20505 T26231 T26232 det a,combination
R20506 T26232 T26246 nsubjpass combination,used
R20507 T26233 T26232 prep of,combination
R20508 T26234 T26236 det all,oligonucleotides
R20509 T26235 T26236 nummod three,oligonucleotides
R20510 T26236 T26233 pobj oligonucleotides,of
R20511 T26237 T26236 acl containing,oligonucleotides
R20512 T26238 T26239 det the,mutated
R20513 T26239 T26237 dobj mutated,containing
R20514 T26240 T26239 cc or,mutated
R20515 T26241 T26244 det the,sites
R20516 T26242 T26244 amod wild-type,sites
R20517 T26243 T26244 amod binding,sites
R20518 T26244 T26246 nsubjpass sites,used
R20519 T26245 T26246 auxpass was,used
R20520 T26246 T26246 ROOT used,used
R20521 T26247 T26246 cc or,used
R20522 T26248 T26249 amod single,oligonucleotides
R20523 T26249 T26251 nsubjpass oligonucleotides,used
R20524 T26250 T26251 auxpass were,used
R20525 T26251 T26246 conj used,used
R20526 T26252 T26251 prep in,used
R20527 T26253 T26254 det the,assay
R20528 T26254 T26252 pobj assay,in
R20529 T26255 T26246 punct .,used
R20530 T26256 T26268 prep After,added
R20531 T26257 T26259 nummod 3,steps
R20532 T26258 T26259 compound washing,steps
R20533 T26259 T26256 pobj steps,After
R20534 T26260 T26259 prep with,steps
R20535 T26261 T26262 compound washing,buffer
R20536 T26262 T26260 pobj buffer,with
R20537 T26263 T26266 punct ",",extract
R20538 T26264 T26266 det the,extract
R20539 T26265 T26266 amod nuclear,extract
R20540 T26266 T26268 nsubjpass extract,added
R20541 T26267 T26268 auxpass was,added
R20542 T26268 T26268 ROOT added,added
R20543 T26269 T26275 punct (,µl
R20544 T26270 T26275 nmod concentration,µl
R20545 T26271 T26270 nummod >,concentration
R20546 T26272 T26273 nummod 0.2,µg
R20547 T26273 T26275 compound µg,µl
R20548 T26274 T26275 compound /,µl
R20549 T26275 T26268 advmod µl,added
R20550 T26276 T26275 punct ),µl
R20551 T26277 T26275 cc and,µl
R20552 T26278 T26279 compound incubated,overnight
R20553 T26279 T26275 conj overnight,µl
R20554 T26280 T26268 prep at,added
R20555 T26281 T26282 nummod 4,°
R20556 T26282 T26280 pobj °,at
R20557 T26283 T26268 npadvmod C,added
R20558 T26284 T26268 punct .,added
R20559 T26285 T26286 det The,lysates
R20560 T26286 T26288 nsubjpass lysates,incubated
R20561 T26287 T26288 auxpass were,incubated
R20562 T26288 T26288 ROOT incubated,incubated
R20563 T26289 T26288 prep with,incubated
R20564 T26290 T26291 nummod 10,µg
R20565 T26291 T26289 pobj µg,with
R20566 T26292 T26291 prep of,µg
R20567 T26293 T26294 amod poly-deoxyinosinic-deoxycytidylic,acid
R20568 T26294 T26292 pobj acid,of
R20569 T26295 T26296 punct (,Sigma-Aldrich
R20570 T26296 T26291 appos Sigma-Aldrich,µg
R20571 T26297 T26291 punct ),µg
R20572 T26298 T26288 punct .,incubated
R20573 T26299 T26300 det The,plate
R20574 T26300 T26302 nsubjpass plate,washed
R20575 T26301 T26302 auxpass was,washed
R20576 T26302 T26302 ROOT washed,washed
R20577 T26303 T26302 prep with,washed
R20578 T26304 T26305 compound HKMG,buffer
R20579 T26305 T26303 pobj buffer,with
R20580 T26306 T26305 cc and,buffer
R20581 T26307 T26305 conj incubated,buffer
R20582 T26308 T26307 prep with,incubated
R20583 T26309 T26311 det a,dilution
R20584 T26310 T26311 nummod 1:1000,dilution
R20585 T26311 T26308 pobj dilution,with
R20586 T26312 T26311 prep of,dilution
R20587 T26313 T26314 compound rabbit,anti-RUNX1
R20588 T26314 T26312 pobj anti-RUNX1,of
R20589 T26315 T26314 punct (,anti-RUNX1
R20590 T26316 T26314 appos ab11903,anti-RUNX1
R20591 T26317 T26314 punct ",",anti-RUNX1
R20592 T26318 T26314 appos Abcam,anti-RUNX1
R20593 T26319 T26314 punct ),anti-RUNX1
R20594 T26320 T26311 cc or,dilution
R20595 T26321 T26322 nummod 1:200,dilution
R20596 T26322 T26311 conj dilution,dilution
R20597 T26323 T26322 prep of,dilution
R20598 T26324 T26325 compound rabbit,anti-RUNX3
R20599 T26325 T26323 pobj anti-RUNX3,of
R20600 T26326 T26327 punct (,H-50
R20601 T26327 T26325 appos H-50,anti-RUNX3
R20602 T26328 T26327 punct ",",H-50
R20603 T26329 T26331 compound Santa,Biotechnology
R20604 T26330 T26331 compound Cruz,Biotechnology
R20605 T26331 T26327 appos Biotechnology,H-50
R20606 T26332 T26333 punct ",",Inc.
R20607 T26333 T26331 appos Inc.,Biotechnology
R20608 T26334 T26331 punct ),Biotechnology
R20609 T26335 T26311 prep at,dilution
R20610 T26336 T26337 nummod 4,°
R20611 T26337 T26335 pobj °,at
R20612 T26338 T26307 conj C,incubated
R20613 T26339 T26338 prep for,C
R20614 T26340 T26341 nummod 2,h
R20615 T26341 T26339 pobj h,for
R20616 T26342 T26302 punct .,washed
R20617 T26343 T26369 prep After,added
R20618 T26344 T26346 nummod three,steps
R20619 T26345 T26346 amod washing,steps
R20620 T26346 T26343 pobj steps,After
R20621 T26347 T26346 prep with,steps
R20622 T26348 T26349 compound HKMG,buffer
R20623 T26349 T26347 pobj buffer,with
R20624 T26350 T26349 punct ",",buffer
R20625 T26351 T26353 det a,antibody
R20626 T26352 T26353 amod secondary,antibody
R20627 T26353 T26349 appos antibody,buffer
R20628 T26354 T26369 punct (,added
R20629 T26355 T26369 nsubjpass anti,added
R20630 T26356 T26357 compound rabbit,IgG-HRP
R20631 T26357 T26369 nsubjpass IgG-HRP,added
R20632 T26358 T26357 punct ",",IgG-HRP
R20633 T26359 T26357 appos "1:3,000",IgG-HRP
R20634 T26360 T26359 prep in,"1:3,000"
R20635 T26361 T26362 compound HKMG,buffer
R20636 T26362 T26360 pobj buffer,in
R20637 T26363 T26357 punct ",",IgG-HRP
R20638 T26364 T26366 compound Cell,Technology
R20639 T26365 T26366 compound Signaling,Technology
R20640 T26366 T26357 appos Technology,IgG-HRP
R20641 T26367 T26357 punct ),IgG-HRP
R20642 T26368 T26369 auxpass was,added
R20643 T26369 T26369 ROOT added,added
R20644 T26370 T26369 punct ",",added
R20645 T26371 T26369 cc and,added
R20646 T26372 T26373 det the,plate
R20647 T26373 T26375 nsubjpass plate,incubated
R20648 T26374 T26375 auxpass was,incubated
R20649 T26375 T26369 conj incubated,added
R20650 T26376 T26375 prep for,incubated
R20651 T26377 T26378 nummod 1,h
R20652 T26378 T26376 pobj h,for
R20653 T26379 T26375 prep at,incubated
R20654 T26380 T26381 nummod 4,°
R20655 T26381 T26379 pobj °,at
R20656 T26382 T26375 conj C,incubated
R20657 T26383 T26369 punct .,added
R20658 T26384 T26385 det The,wells
R20659 T26385 T26387 nsubjpass wells,washed
R20660 T26386 T26387 auxpass were,washed
R20661 T26387 T26387 ROOT washed,washed
R20662 T26388 T26389 nummod 4,times
R20663 T26389 T26387 npadvmod times,washed
R20664 T26390 T26387 prep with,washed
R20665 T26391 T26392 compound HKMG,buffer
R20666 T26392 T26390 pobj buffer,with
R20667 T26393 T26387 prep before,washed
R20668 T26394 T26393 pcomp adding,before
R20669 T26395 T26397 det the,reagent
R20670 T26396 T26397 compound substrate,reagent
R20671 T26397 T26394 dobj reagent,adding
R20672 T26398 T26400 punct (,Systems
R20673 T26399 T26400 compound R&D,Systems
R20674 T26400 T26397 appos Systems,reagent
R20675 T26401 T26400 punct ),Systems
R20676 T26402 T26387 punct .,washed
R20677 T26403 T26405 det The,reaction
R20678 T26404 T26405 amod colorimetric,reaction
R20679 T26405 T26407 nsubjpass reaction,stopped
R20680 T26406 T26407 auxpass was,stopped
R20681 T26407 T26407 ROOT stopped,stopped
R20682 T26408 T26407 prep by,stopped
R20683 T26409 T26408 pcomp adding,by
R20684 T26410 T26412 nummod 2,H2SO4
R20685 T26411 T26412 compound M,H2SO4
R20686 T26412 T26409 dobj H2SO4,adding
R20687 T26413 T26407 punct .,stopped
R20688 T26414 T26419 nsubjpass Absorbance,measured
R20689 T26415 T26414 prep at,Absorbance
R20690 T26416 T26417 nummod 450,nm
R20691 T26417 T26415 pobj nm,at
R20692 T26418 T26419 auxpass was,measured
R20693 T26419 T26419 ROOT measured,measured
R20694 T26420 T26419 advcl using,measured
R20695 T26421 T26423 det a,reader
R20696 T26422 T26423 amod microplate,reader
R20697 T26423 T26420 dobj reader,using
R20698 T26424 T26423 punct (,reader
R20699 T26425 T26426 compound Berthold,Technologies
R20700 T26426 T26423 appos Technologies,reader
R20701 T26427 T26423 punct ),reader
R20702 T26428 T26419 punct .,measured

GO-MF

Id Subject Object Predicate Lexical cue
T26429 402-409 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T26430 591-598 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T26431 1158-1166 http://purl.obolibrary.org/obo/GO_0003823 denotes antibody

GO-CC

Id Subject Object Predicate Lexical cue
T26432 75-80 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T26433 1213-1217 http://purl.obolibrary.org/obo/GO_0005623 denotes Cell
T26434 1158-1166 http://purl.obolibrary.org/obo/GO_0019815 denotes antibody
T26435 1158-1166 http://purl.obolibrary.org/obo/GO_0042571 denotes antibody

sentences

Id Subject Object Predicate Lexical cue
T25839 0-29 Sentence denotes Promoter enzyme immuno assay.
T25840 30-141 Sentence denotes As performed in the pull-down assay, HEK293T cells were transfected with RUNX1 or RUNX3 and subsequently lysed.
T25841 142-191 Sentence denotes Insoluble material was removed by centrifugation.
T25842 192-329 Sentence denotes 384-well plates, precoated with streptavidin (Thermo Fisher Scientific) were washed 3 times with washing buffer (PBS and 0.05% Tween 20).
T25843 330-499 Sentence denotes Biotinylated FOXP3 promoter/oligonucleotides probes containing the RUNX binding sites were added (1 pmol per well; 50 fmol/µl) and incubated for 1 h at room temperature.
T25844 500-664 Sentence denotes Either a combination of all three oligonucleotides containing the mutated or the wild-type binding sites was used or single oligonucleotides were used in the assay.
T25845 665-894 Sentence denotes After 3 washing steps with washing buffer, the nuclear extract was added (concentration > 0.2 µg/µl) and incubated overnight at 4°C. The lysates were incubated with 10 µg of poly-deoxyinosinic-deoxycytidylic acid (Sigma-Aldrich).
T25846 895-1101 Sentence denotes The plate was washed with HKMG buffer and incubated with a 1:1000 dilution of rabbit anti-RUNX1 (ab11903, Abcam) or 1:200 dilution of rabbit anti-RUNX3 (H-50, Santa Cruz Biotechnology, Inc.) at 4°C for 2 h.
T25847 1102-1392 Sentence denotes After three washing steps with HKMG buffer, a secondary antibody (anti–rabbit IgG-HRP, 1:3,000 in HKMG buffer, Cell Signaling Technology) was added, and the plate was incubated for 1 h at 4°C. The wells were washed 4 times with HKMG buffer before adding the substrate reagent (R&D Systems).
T25848 1393-1451 Sentence denotes The colorimetric reaction was stopped by adding 2 M H2SO4.
T25849 1452-1536 Sentence denotes Absorbance at 450 nm was measured using a microplate reader (Berthold Technologies).
T362 0-29 Sentence denotes Promoter enzyme immuno assay.
T363 30-141 Sentence denotes As performed in the pull-down assay, HEK293T cells were transfected with RUNX1 or RUNX3 and subsequently lysed.
T364 142-191 Sentence denotes Insoluble material was removed by centrifugation.
T365 192-329 Sentence denotes 384-well plates, precoated with streptavidin (Thermo Fisher Scientific) were washed 3 times with washing buffer (PBS and 0.05% Tween 20).
T366 330-499 Sentence denotes Biotinylated FOXP3 promoter/oligonucleotides probes containing the RUNX binding sites were added (1 pmol per well; 50 fmol/µl) and incubated for 1 h at room temperature.
T367 500-664 Sentence denotes Either a combination of all three oligonucleotides containing the mutated or the wild-type binding sites was used or single oligonucleotides were used in the assay.
T368 665-797 Sentence denotes After 3 washing steps with washing buffer, the nuclear extract was added (concentration > 0.2 µg/µl) and incubated overnight at 4°C.
T369 798-894 Sentence denotes The lysates were incubated with 10 µg of poly-deoxyinosinic-deoxycytidylic acid (Sigma-Aldrich).
T370 895-1101 Sentence denotes The plate was washed with HKMG buffer and incubated with a 1:1000 dilution of rabbit anti-RUNX1 (ab11903, Abcam) or 1:200 dilution of rabbit anti-RUNX3 (H-50, Santa Cruz Biotechnology, Inc.) at 4°C for 2 h.
T371 1102-1294 Sentence denotes After three washing steps with HKMG buffer, a secondary antibody (anti–rabbit IgG-HRP, 1:3,000 in HKMG buffer, Cell Signaling Technology) was added, and the plate was incubated for 1 h at 4°C.
T372 1295-1392 Sentence denotes The wells were washed 4 times with HKMG buffer before adding the substrate reagent (R&D Systems).
T373 1393-1451 Sentence denotes The colorimetric reaction was stopped by adding 2 M H2SO4.
T374 1452-1536 Sentence denotes Absorbance at 450 nm was measured using a microplate reader (Berthold Technologies).

events-check-again

Id Subject Object Predicate Lexical cue
T26459 67-80 Protein denotes HEK293T cells
T26460 86-97 Gene_expression denotes transfected
T26461 86-97 Gene_expression denotes transfected
T26462 103-108 Protein denotes RUNX1
T26463 112-117 Protein denotes RUNX3
T26464 343-348 Protein denotes FOXP3
T26465 985-990 Protein denotes RUNX1
T26466 1041-1046 Protein denotes RUNX3
R20709 T26462 T26460 themeOf RUNX1,transfected
R20710 T26463 T26461 themeOf RUNX3,transfected

bionlp-st-ge-2016-reference-tees

Id Subject Object Predicate Lexical cue
T26444 103-108 Protein denotes RUNX1
T26445 112-117 Protein denotes RUNX3
T26446 86-97 Gene_expression denotes transfected
T26447 86-97 Gene_expression denotes transfected
T26448 343-348 Protein denotes FOXP3
T26449 1180-1183 Protein denotes IgG
T26450 1184-1187 Protein denotes HRP
R20705 T26444 T26446 themeOf RUNX1,transfected
R20706 T26445 T26447 themeOf RUNX3,transfected

bionlp-st-ge-2016-reference

Id Subject Object Predicate Lexical cue
T25831 67-80 Protein denotes HEK293T cells
T25832 86-97 Gene_expression denotes transfected
T25833 86-97 Gene_expression denotes transfected
T25834 103-108 Protein denotes RUNX1
T25835 112-117 Protein denotes RUNX3
T25836 343-348 Protein denotes FOXP3
T25837 985-990 Protein denotes RUNX1
T25838 1041-1046 Protein denotes RUNX3
R20132 T25834 T25832 themeOf RUNX1,transfected
R20133 T25835 T25833 themeOf RUNX3,transfected

bionlp-st-ge-2016-uniprot

Id Subject Object Predicate Lexical cue
T26122 103-108 Q01196 denotes RUNX1
T26123 112-117 Q13761 denotes RUNX3
T26124 343-348 Q9BZS1 denotes FOXP3
T26125 397-401 Q01196 denotes RUNX
T26126 397-401 Q13950 denotes RUNX
T26127 397-401 Q13761 denotes RUNX
T26128 985-990 Q01196 denotes RUNX1
T26129 1041-1046 Q13761 denotes RUNX3

test2

Id Subject Object Predicate Lexical cue
T25824 67-80 Protein denotes HEK293T cells
T25825 86-97 Gene_expression denotes transfected
T25826 103-108 Protein denotes RUNX1
T25827 112-117 Protein denotes RUNX3
T25828 343-348 Protein denotes FOXP3
T25829 985-990 Protein denotes RUNX1
T25830 1041-1046 Protein denotes RUNX3
R20129 T25824 T25825 themeOf HEK293T cells,transfected
R20130 T25826 T25825 themeOf RUNX1,transfected
R20131 T25827 T25825 themeOf RUNX3,transfected