PMC:2806624 / 50074-50875 JSONTXT

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{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/2806624","sourcedb":"PMC","sourceid":"2806624","source_url":"http://www.ncbi.nlm.nih.gov/pmc/2806624","text":"Flow cytometry.\nFor analysis of human FOXP3 expression on the single-cell level, cells were first stained with the monoclonal CD4 mAb (Beckman Coulter), and after fixation and permeabilization, they were incubated with anti–human Foxp3-Alexa Fluor 488 antibody (BioLegend) based on the manufacturer's recommendations and subjected to FACS (EPICS XL-MCL; Beckman Coulter). A mouse IgG1 antibody (BioLegend) was used as an isotype control. Data were analyzed with the CXP software (Beckman Coulter). Cells were cultured with IL-2, and then left unstimulated or stimulated with anti-CD2/-CD3/-CD28 mAb.\nFlow cytometry analyses of the mouse cells were performed on an LSRII (BD) and cell sorting was performed on a FACSAria (BD). 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