10.1371/journal.pone.0008058.g003 Figure 3  Formation of NB-like particles from boiled serum in metastable versus supersaturated medium.
(A) Metastable medium: FBS and 25% HS were boiled at 95°C for the time indicated on the left panel (0, 10, 30, and 120 min). “0 min” refers to control, untreated serum. The boiled sera were then inoculated into DMEM to the concentrations shown on the top heading. (B) Supersaturated medium: both FBS and HS were treated as described in (A), except that 1 mM each of CaCl2 and NaH2PO4 (labeled as “Calcium+Phosphate Added”) was added following the inoculation of the boiled serum into DMEM. Note the marked differences in turbidity changes between the various panels shown in (A) versus (B). See the text for explanation and interpretation.