Energy-Dispersive X-Ray Spectroscopy
Washed particles, calcium granules, and NB specimens were resuspended in double-distilled water and deposited on formvar carbon-coated grids. The grids were dried overnight in a laminar flow hood. The samples were observed with a SEM S-3000N scanning electron microscope (Hitachi Science Systems) and the energy-dispersive X-ray spectroscopy (EDX) analysis was performed using an EMAX Energy EX-400 EDX device (Horiba, Tokyo, Japan). Each sample was irradiated for 30 sec and data acquisition was performed with the EMAX software (Horiba) in point mode analysis. Three different areas of each sample were analyzed to ensure homogenous readings.