Fig. 4 Methyl order parameters measured by other methods are plotted against order parameters obtained from RDCs in ubiquitin (a, c, g, e) and against residue position (b, d, f, h) in color ( are in black). a,b are obtained from 2H relaxation experiments (Lee et al. 1999) and sample motions faster than the correlation time. c,d are obtained from the CxH dipolar splitting reduction for ubiquitin in a microcrystalline form using separated local field experiments by solid-state NMR under magic-angle spinning condition.  of CxHx are plotted with the  of CxCmet. These values are obtained by squaring the values reported by (Lorieau and McDermott 2006). The time scale sampled also includes the ns–ms range. e,f obtained from rotameric fitting of χ1 based on averaged 3JNCγ- and 3JC′Cγ-couplings and on CH RDCs according to (Chou et al. 2003). These order parameters are based on a finite rotameric jump model and sample dynamics over similar time scales. g,h The same order parameters  are multiplied by S2(NH)LS for the same residue (i, green) and for the following residue (i + 1, red) to include rapid small-scale fluctuations. The Pearson correlation r (with p value) and rmsd fit are also given