Methyl group a order parameters,  and b amplitude of anisotropy, ηrdc measured from RDCs plotted against residue number in human ubiquitin. Also encoded in the graph are residue type (letter), their solvent exposure (grey core exposed; white solvent exposed) and the distance of the methyl group to the backbone (blueCβ; greenCγ; orangeCδ; redCε). RDC-based order parameters have a mean value of 0.43 with a standard deviation of 0.25