Fig. 5  The N-terminal domain of Nab2 does not bind to RNA. Binding to radioactively labeled randomized sequence 25-nt poly(N) RNA probe was assessed by a gel shift assay. Purified recombinant GST-Nab2-N, negative control, GST alone, or positive control, GST-SRm160-N, was incubated with radioactively labeled RNA probe. Complexes were resolved by native gel electrophoresis. Results are shown for probe alone (−), GST alone, or increasing concentrations (0.5–5 μM) of GST-Nab2-N or GST-SRm160-N. The positions of the free probe and bound complexes are indicated.