FADD DD variant  ΔGD-N (kcal mol-1)  ΔΔGD-N (kcal mol-1)  mD-N (kcal mol-1 M-1)  kf2 M (s-1)  Φ2 M
Helix 1
Cys98Ala  7.2   1.8  48
Cys98Gly  6.2  1.0  1.6  26  0.38
Asn102Ala  6.9   1.3  58
Asn102Gly  6.3  0.7  1.3  33  0.50
Helix2
Arg113Ala  6.5   1.2  52
Arg113Gly  5.4  1.1  1.1  38  0.17
Arg114Ala  6.4   1.6  46
Arg114Gly  5.0  1.4  1.6  28  0.20
Arg117Ala  6.3   1.2  47
Arg117Gly  5.2  1.1  1.1  31  0.23
Helix3
Asp127Ala  7.2   1.5  49
Asp127Gly  6.2  1.0  1.4  45  0.07
Ser128Ala  7.0   1.1  37
Ser128Gly  6.6  0.43  1.4  47  n.d.
Asp131Ala  7.5   1.4  44
Asp131Gly  6.5  1.0  1.4  46  –0.03
Arg132Ala  6.6   1.2  40
Arg132Gly  5.5  1.1  1.1  43  –0.04
Helix4
Thr138Ala  7.1   1.4  68
Thr138Gly  6.4  0.7  1.5  35  0.56
Glu139Ala  6.4   1.4  29
Glu139Gly  5.7  0.7  1.3  16  0.49
Arg142Ala  5.9   1.3  32
Arg142Gly  4.8  1.1  1.3  12  0.51
Glu143Ala  6.6   1.4  34
Glu143Gly  4.2  2.4  1.7  10  0.29
Arg146Ala  6.3    30
Arg146Gly  5.1  1.2  20  20  0.19
Asn150Ala  6.8   2.0  46
Asn150Gly  6.2  0.6  1.5  32  0.35
Helix5
Arg166Ala  4.6   1.3  22
Arg166Gly  3.7  0.9  1.6  13  0.30
Ser167Ala  7.6   1.7  69
Ser167Gly  6.4  1.3  1.7  28  0.42
Helix6
Asp175Ala  6.4   1.4  38
Asp175Gly  5.4  1.0  1.5  23  0.12
Gln178Ala  6.6   1.2  49
Gln178Gly  5.6  1.0  1.4  27  0.31