Mutation Analysis of the FOXF1 Gene
Overlapping amplicons covering the entire coding region of exons 1 and 2 of FOXF1 were amplified and sequenced by conventional Sanger di-deoxynucleotide sequencing (Lone Star Labs, Houston, TX; Molecular Core Lab, BCM, Houston, TX). DNA sequences were analyzed by comparison with reference sequence (NM_001451.2) with the use of Sequencher v4.2 (GeneCodes, Ann Arbor, MI, USA). None of the identified mutations was found in 150 ethnically matched control chromosomes. Individual primer sequences and PCR conditions are available on request.