Long-Range Polymerase Chain Reaction and DNA Sequencing
Long-range polymerase chain reaction (LR-PCR), performed in accordance with the manufacturer's instructions (Takara Bio, Japan), amplified the predicted junction fragments from the breakpoint regions in patients D1–D9. PCR products were purified with the PCR Purification Kit (QIAGEN, Valencia, CA, USA) and bidirectionally sequenced by Sanger di-deoxynucleotide sequencing (Lone Star Labs, Houston, TX, USA).