Intranasal delivery of antiretroviral drugs has been proposed as a potential strategy to overcome the poor penetration of these drugs into the brain and to target HIV that harbours in the CNS. The intranasal administration of the viral entry inhibitor peptide T has recently been explored for the prevention of neuro-AIDS development such as cognitive impairment associated with HIV. As well as being an entry inhibitor, Peptide T reduces the initial infection of cells expressing CCR5 receptors such as monocytes and microglia and also acts as an antagonist of free gp120 and thereby reduces toxic effects (Hanson and Frey, 2007). More interestingly, peptide T has demonstrated antiviral and immunological benefits in HIV patients receiving intranasal peptide T with decreased viral load in monocyte reservoirs, increased antiviral cytotoxic T-cells with no drug-related toxicity and increased CD4 (Polianova et al., 2003).