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mononuclear cells were stimulated with LPS (1 μg/mL), in the presence and absence of Pentoxifylline (PTX; 20 mM), a nonspecific phosphodiesterase inhibitor. Western blotting of phosphorylated cytoplasmic I-κBα, nuclear factor-κB p65 (NF-κB), and nuclear cAMP-response element binding protein (CREB) was performed. DNA binding of NF-κB and CREB was verified by electrophoretic mobility shift assay. TNF-α levels were determined in the supernatant of stimulated cells in the presence and absence Protein kinase A inhibition by an enzyme-linked immunosorbent assay (ELISA)."}

{"project":"GO-BP","denotations":[{"id":"T840","span":{"begin":142,"end":159},"obj":"http://purl.obolibrary.org/obo/GO_0008081"},{"id":"T858","span":{"begin":142,"end":169},"obj":"http://purl.obolibrary.org/obo/GO_0051344"},{"id":"T859","span":{"begin":268,"end":281},"obj":"http://purl.obolibrary.org/obo/GO_0051591"}],"text":"METHODS\nHuman mononuclear cells were stimulated with LPS (1 μg/mL), in the presence and absence of Pentoxifylline (PTX; 20 mM), a nonspecific phosphodiesterase inhibitor. Western blotting of phosphorylated cytoplasmic I-κBα, nuclear factor-κB p65 (NF-κB), and nuclear cAMP-response element binding protein (CREB) was performed. DNA binding of NF-κB and CREB was verified by electrophoretic mobility shift assay. TNF-α levels were determined in the supernatant of stimulated cells in the presence and absence Protein kinase A inhibition by an enzyme-linked immunosorbent assay (ELISA)."}

{"project":"GO-MF","denotations":[{"id":"T867","span":{"begin":142,"end":159},"obj":"http://purl.obolibrary.org/obo/GO_0008081"},{"id":"T870","span":{"begin":290,"end":297},"obj":"http://purl.obolibrary.org/obo/GO_0005488"},{"id":"T871","span":{"begin":332,"end":339},"obj":"http://purl.obolibrary.org/obo/GO_0005488"},{"id":"T875","span":{"begin":290,"end":305},"obj":"http://purl.obolibrary.org/obo/GO_0005515"},{"id":"T876","span":{"begin":328,"end":339},"obj":"http://purl.obolibrary.org/obo/GO_0003677"},{"id":"T880","span":{"begin":332,"end":348},"obj":"http://purl.obolibrary.org/obo/GO_0051059"}],"text":"METHODS\nHuman mononuclear cells were stimulated with LPS (1 μg/mL), in the presence and absence of Pentoxifylline (PTX; 20 mM), a nonspecific phosphodiesterase inhibitor. Western blotting of phosphorylated cytoplasmic I-κBα, nuclear factor-κB p65 (NF-κB), and nuclear cAMP-response element binding protein (CREB) was performed. DNA binding of NF-κB and CREB was verified by electrophoretic mobility shift assay. TNF-α levels were determined in the supernatant of stimulated cells in the presence and absence Protein kinase A inhibition by an enzyme-linked immunosorbent assay (ELISA)."}

{"project":"GO-CC","denotations":[{"id":"T882","span":{"begin":26,"end":31},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T883","span":{"begin":474,"end":479},"obj":"http://purl.obolibrary.org/obo/GO_0005623"}],"text":"METHODS\nHuman mononuclear cells were stimulated with LPS (1 μg/mL), in the presence and absence of Pentoxifylline (PTX; 20 mM), a nonspecific phosphodiesterase inhibitor. Western blotting of phosphorylated cytoplasmic I-κBα, nuclear factor-κB p65 (NF-κB), and nuclear cAMP-response element binding protein (CREB) was performed. DNA binding of NF-κB and CREB was verified by electrophoretic mobility shift assay. TNF-α levels were determined in the supernatant of stimulated cells in the presence and absence Protein kinase A inhibition by an enzyme-linked immunosorbent assay (ELISA)."}

{"project":"sentences","denotations":[{"id":"T117","span":{"begin":0,"end":7},"obj":"Sentence"},{"id":"T118","span":{"begin":8,"end":170},"obj":"Sentence"},{"id":"T119","span":{"begin":171,"end":327},"obj":"Sentence"},{"id":"T120","span":{"begin":328,"end":411},"obj":"Sentence"},{"id":"T121","span":{"begin":412,"end":584},"obj":"Sentence"},{"id":"T9","span":{"begin":0,"end":7},"obj":"Sentence"},{"id":"T10","span":{"begin":8,"end":170},"obj":"Sentence"},{"id":"T11","span":{"begin":171,"end":327},"obj":"Sentence"},{"id":"T12","span":{"begin":328,"end":411},"obj":"Sentence"},{"id":"T13","span":{"begin":412,"end":584},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"METHODS\nHuman mononuclear cells were stimulated with LPS (1 μg/mL), in the presence and absence of Pentoxifylline (PTX; 20 mM), a nonspecific phosphodiesterase inhibitor. Western blotting of phosphorylated cytoplasmic I-κBα, nuclear factor-κB p65 (NF-κB), and nuclear cAMP-response element binding protein (CREB) was performed. DNA binding of NF-κB and CREB was verified by electrophoretic mobility shift assay. TNF-α levels were determined in the supernatant of stimulated cells in the presence and absence Protein kinase A inhibition by an enzyme-linked immunosorbent assay (ELISA)."}

{"project":"events-check-again","denotations":[{"id":"T898","span":{"begin":191,"end":205},"obj":"Phosphorylation"},{"id":"T899","span":{"begin":218,"end":223},"obj":"Protein"},{"id":"T900","span":{"begin":243,"end":246},"obj":"Protein"},{"id":"T901","span":{"begin":412,"end":417},"obj":"Protein"}],"relations":[{"id":"R657","pred":"themeOf","subj":"T899","obj":"T898"}],"text":"METHODS\nHuman mononuclear cells were stimulated with LPS (1 μg/mL), in the presence and absence of Pentoxifylline (PTX; 20 mM), a nonspecific phosphodiesterase inhibitor. Western blotting of phosphorylated cytoplasmic I-κBα, nuclear factor-κB p65 (NF-κB), and nuclear cAMP-response element binding protein (CREB) was performed. DNA binding of NF-κB and CREB was verified by electrophoretic mobility shift assay. TNF-α levels were determined in the supernatant of stimulated cells in the presence and absence Protein kinase A inhibition by an enzyme-linked immunosorbent assay (ELISA)."}

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