Antibodies for phosphorylated CREB (serine 133), phosphorylated I-κBα (serines 32 and 36), phosphorylated NF-κB p65 subunit (serine 276), and secondary antibodies were purchased from Cell Signaling (Beverly, MA).
Dextran T500 and Percoll were received from GE Healthcare/Amersham Biosciences (Piscataway, NJ).