PMC:2664230 / 4921-13845 JSON TXT

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bionlp-st-ge-2016-reference

Id	Subject	Object	Predicate	Lexical cue
T2441	746-760	Phosphorylation	denotes	phosphorylated
T2442	761-765	Protein	denotes	CREB
T2443	767-777	Entity	denotes	serine 133
T2444	780-794	Phosphorylation	denotes	phosphorylated
T2445	780-794	Phosphorylation	denotes	phosphorylated
T2446	795-800	Protein	denotes	I-κBα
T2447	802-812	Entity	denotes	serines 32
T2448	817-819	Entity	denotes	36
T2449	822-836	Phosphorylation	denotes	phosphorylated
T2450	843-846	Protein	denotes	p65
T2451	856-866	Entity	denotes	serine 276
T2452	1091-1096	Protein	denotes	TNF-α
T3248	3296-3303	Positive_regulation	denotes	induced
T3249	3304-3309	Protein	denotes	TNF-α
T3250	3310-3320	Gene_expression	denotes	production
T3251	3473-3486	Positive_regulation	denotes	up-regulation
T3252	3491-3506	Negative_regulation	denotes	down-regulation
T3253	3510-3515	Protein	denotes	TNF-α
T3254	3516-3526	Gene_expression	denotes	production
T4131	3919-3924	Protein	denotes	I-κBα
T4132	3932-3935	Protein	denotes	p65
T4133	3941-3945	Protein	denotes	CREB
T4134	4908-4922	Phosphorylation	denotes	phosphorylated
T4135	4923-4928	Protein	denotes	I-κBα
T4136	4997-5011	Phosphorylation	denotes	phosphorylated
T4137	5018-5021	Protein	denotes	p65
T5627	7699-7704	Protein	denotes	TNF-α
T5628	7705-7715	Gene_expression	denotes	production
T5629	7745-7756	Regulation	denotes	involvement
T5630	7764-7775	Negative_regulation	denotes	attenuation
T5631	7783-7790	Positive_regulation	denotes	induced
T5632	7791-7796	Protein	denotes	TNF-α
T5633	7797-7807	Gene_expression	denotes	production
T5634	8383-8388	Protein	denotes	TNF-α
T3964	3605-3612	Positive_regulation	denotes	induced
T3965	3613-3618	Protein	denotes	TNF-α
T3966	3619-3629	Gene_expression	denotes	production
T3967	3672-3677	Protein	denotes	TNF-α
T5622	7607-7611	Regulation	denotes	Role
T5623	7626-7633	Positive_regulation	denotes	induced
T5624	7651-7656	Protein	denotes	TNF-α
T5625	7657-7667	Gene_expression	denotes	production
T5626	7684-7688	Regulation	denotes	role
R1883	T2443	T2441	themeOf	serine 133,phosphorylated
R1884	T2443	T2442	partOf	serine 133,CREB
R1885	T2447	T2445	themeOf	serines 32,phosphorylated
R1886	T2447	T2446	partOf	serines 32,I-κBα
R1887	T2448	T2444	themeOf	36,phosphorylated
R1888	T2448	T2446	partOf	36,I-κBα
R1889	T2451	T2449	themeOf	serine 276,phosphorylated
R1890	T2451	T2450	partOf	serine 276,p65
R4667	T5623	T5622	themeOf	induced,Role
R4668	T5624	T5625	themeOf	TNF-α,production
R4669	T5625	T5623	themeOf	production,induced
R4670	T5627	T5628	themeOf	TNF-α,production
R4671	T5628	T5626	themeOf	production,role
R4672	T5630	T5629	themeOf	attenuation,involvement
R4673	T5631	T5630	themeOf	induced,attenuation
R4674	T5632	T5633	themeOf	TNF-α,production
R4675	T5633	T5631	themeOf	production,induced
R3371	T4135	T4134	themeOf	I-κBα,phosphorylated
R3372	T4137	T4136	themeOf	p65,phosphorylated
R2595	T3249	T3250	themeOf	TNF-α,production
R2596	T3250	T3248	themeOf	production,induced
R2597	T3253	T3254	themeOf	TNF-α,production
R2598	T3254	T3251	themeOf	production,up-regulation
R2599	T3254	T3252	themeOf	production,down-regulation
R3248	T3965	T3966	themeOf	TNF-α,production
R3249	T3966	T3964	themeOf	production,induced

2_test

Id	Subject	Object	Predicate	Lexical cue
18568240-17689696-22101119	2066-2068	17689696	denotes	10
18568240-17689696-22101120	8191-8193	17689696	denotes	10

pmc-enju-pas

Id	Subject	Object	Predicate	Lexical cue
T2794	37-45	VB	denotes	approved
T2795	46-48	IN	denotes	by
T2796	49-52	DT	denotes	the
T2797	53-58	NNP	denotes	Human
T2798	59-67	NNP	denotes	Research
T2799	68-79	NNP	denotes	Protections
T2800	80-87	NNP	denotes	Program
T2801	88-91	CC	denotes	and
T2802	92-95	DT	denotes	the
T2803	96-109	NNP	denotes	Institutional
T2804	110-116	NNP	denotes	Review
T2805	117-123	NNP	denotes	Board.
T2806	124-131	NNP	denotes	Written
T2807	132-139	NN	denotes	consent
T2808	140-142	TO	denotes	to
T2809	143-154	VB	denotes	participate
T2810	155-157	IN	denotes	in
T2811	158-161	DT	denotes	the
T2812	162-167	NN	denotes	study
T2813	168-171	VB	denotes	was
T2814	172-180	VB	denotes	obtained
T2815	181-185	IN	denotes	from
T2816	186-189	DT	denotes	all
T2817	190-200	NN	denotes	volunteers
T2818	201-206	RB	denotes	prior
T2819	207-209	TO	denotes	to
T2820	210-215	NN	denotes	blood
T2821	216-237	JJ	denotes	donation. Heparinized
T2822	238-249	NN	denotes	Vacutainers
T2823	250-254	VB	denotes	were
T2824	255-264	VB	denotes	purchased
T2825	265-269	IN	denotes	from
T2826	270-276	NNP	denotes	Becton
T2827	277-286	NNP	denotes	Dickinson
T2828	287-288	-LRB-	denotes	(
T2829	288-291	NNP	denotes	San
T2830	292-296	NNP	denotes	Jose
T2831	296-297	-COMMA-	denotes	,
T2832	298-300	NNP	denotes	CA
T2833	300-301	-RRB-	denotes	)
T2834	303-309	NN	denotes	1.5-mL
T2835	310-319	NNP	denotes	Eppendorf
T2836	320-330	NN	denotes	centrifuge
T2837	331-336	NN	denotes	tubes
T2838	337-341	VB	denotes	were
T2839	342-351	VB	denotes	purchased
T2840	352-356	IN	denotes	from
T2841	357-363	NNP	denotes	Fisher
T2842	364-374	NNP	denotes	Scientific
T2843	375-376	-LRB-	denotes	(
T2844	376-385	NNP	denotes	Pittsburg
T2845	385-386	-COMMA-	denotes	,
T2846	387-389	NNP	denotes	PA
T2847	389-390	-RRB-	denotes	)
T2848	392-398	NN	denotes	Hank’s
T2849	399-407	JJ	denotes	balanced
T2850	408-412	NN	denotes	salt
T2851	413-421	NN	denotes	solution
T2852	422-423	-LRB-	denotes	(
T2853	423-427	NN	denotes	HBSS
T2854	427-428	-RRB-	denotes	)
T2855	429-432	CC	denotes	and
T2856	433-437	NN	denotes	RPMI
T2857	438-442	CD	denotes	1640
T2858	443-447	VB	denotes	were
T2859	448-456	VB	denotes	obtained
T2860	457-461	IN	denotes	from
T2861	462-468	NNP	denotes	Irvine
T2862	469-479	NNP	denotes	Scientific
T2863	480-481	-LRB-	denotes	(
T2864	481-486	NNP	denotes	Santa
T2865	487-490	NNP	denotes	Ana
T2866	490-491	-COMMA-	denotes	,
T2867	492-494	NNP	denotes	CA
T2868	494-495	-RRB-	denotes	)
T2869	497-500	NN	denotes	LPS
T2870	501-517	NN	denotes	from Escherichia
T2871	518-531	NN	denotes	coli serotype
T2872	532-536	CD	denotes	0111
T2873	536-537	-COLON-	denotes	:
T2874	537-539	NN	denotes	B4
T2875	540-543	CC	denotes	and
T2876	544-547	NN	denotes	PTX
T2877	548-552	VB	denotes	were
T2878	553-562	VB	denotes	purchased
T2879	563-567	IN	denotes	from
T2880	568-573	NN	denotes	Sigma
T2881	574-575	-LRB-	denotes	(
T2882	575-578	NNP	denotes	St.
T2883	579-584	NNP	denotes	Louis
T2884	584-585	-COMMA-	denotes	,
T2885	586-588	NN	denotes	MO
T2886	588-589	-RRB-	denotes	)
T2887	591-594	DT	denotes	The
T2888	595-598	NN	denotes	PKA
T2889	599-608	NN	denotes	inhibitor
T2890	608-609	-COMMA-	denotes	,
T2891	610-612	NN	denotes	N-
T2892	612-613	-LRB-	denotes	[
T2893	613-615	CD	denotes	2-
T2894	615-616	-LRB-	denotes	(
T2895	616-617	-LRB-	denotes	(
T2896	617-632	NN	denotes	p-bromocinnamyl
T2897	632-633	-RRB-	denotes	)
T2898	633-638	NN	denotes	amino
T2899	638-639	-RRB-	denotes	)
T2900	639-644	NN	denotes	ethyl
T2901	644-645	-RRB-	denotes	]
T2902	645-676	NN	denotes	-5-isoguinolinesulfonamide-2HCl
T2903	676-677	-RRB-	denotes	]
T2904	678-679	-LRB-	denotes	(
T2905	679-682	NN	denotes	H89
T2906	682-683	-RRB-	denotes	)
T2907	683-684	-COMMA-	denotes	,
T2908	685-688	VB	denotes	was
T2909	689-698	VB	denotes	purchased
T2910	699-703	IN	denotes	from
T2911	704-714	NN	denotes	Calbiochem
T2912	715-716	-LRB-	denotes	(
T2913	716-718	NNP	denotes	La
T2914	719-724	NNP	denotes	Jolla
T2915	724-725	-COMMA-	denotes	,
T2916	726-728	NNP	denotes	CA
T2917	728-729	-RRB-	denotes	)
T2918	731-741	NN	denotes	Antibodies
T2919	742-745	IN	denotes	for
T2920	746-760	VB	denotes	phosphorylated
T2921	761-765	NN	denotes	CREB
T2922	766-767	-LRB-	denotes	(
T2923	767-773	NN	denotes	serine
T2924	774-777	CD	denotes	133
T2925	777-778	-RRB-	denotes	)
T2926	778-779	-COMMA-	denotes	,
T2927	780-794	VB	denotes	phosphorylated
T2928	795-800	NN	denotes	I-κBα
T2929	801-802	-LRB-	denotes	(
T2930	802-809	NN	denotes	serines
T2931	810-812	CD	denotes	32
T2932	813-816	CC	denotes	and
T2933	817-819	CD	denotes	36
T2934	819-820	-RRB-	denotes	)
T2935	820-821	-COMMA-	denotes	,
T2936	822-836	VB	denotes	phosphorylated
T2937	837-842	NN	denotes	NF-κB
T2938	843-846	NN	denotes	p65
T2939	847-854	NN	denotes	subunit
T2940	855-856	-LRB-	denotes	(
T2941	856-862	NN	denotes	serine
T2942	863-866	CD	denotes	276
T2943	866-867	-RRB-	denotes	)
T2944	867-868	-COMMA-	denotes	,
T2945	869-872	CC	denotes	and
T2946	873-882	JJ	denotes	secondary
T2947	883-893	NN	denotes	antibodies
T2948	894-898	VB	denotes	were
T2949	899-908	VB	denotes	purchased
T2950	909-913	IN	denotes	from
T2951	914-918	NN	denotes	Cell
T2952	919-928	NN	denotes	Signaling
T2953	929-930	-LRB-	denotes	(
T2954	930-937	NNP	denotes	Beverly
T2955	937-938	-COMMA-	denotes	,
T2956	939-941	JJ	denotes	MA
T2957	941-942	-RRB-	denotes	)
T2958	942-951	NN	denotes	. Dextran
T2959	952-956	NN	denotes	T500
T2960	957-960	CC	denotes	and
T2961	961-968	NN	denotes	Percoll
T2962	969-973	VB	denotes	were
T2963	974-982	VB	denotes	received
T2964	983-987	IN	denotes	from
T2965	988-990	NNP	denotes	GE
T2966	991-1010	NNP	denotes	Healthcare/Amersham
T2967	1011-1022	NNP	denotes	Biosciences
T2968	1023-1024	-LRB-	denotes	(
T2969	1024-1034	NNP	denotes	Piscataway
T2970	1034-1035	-COMMA-	denotes	,
T2971	1036-1038	NNP	denotes	NJ
T2972	1038-1039	-RRB-	denotes	)
T2973	1041-1044	DT	denotes	The
T2974	1045-1058	JJ	denotes	enzyme-linked
T2975	1059-1072	JJ	denotes	immunosorbent
T2976	1073-1078	NN	denotes	assay
T2977	1079-1080	-LRB-	denotes	(
T2978	1080-1085	NN	denotes	ELISA
T2979	1085-1086	-RRB-	denotes	)
T2980	1087-1090	IN	denotes	for
T2981	1091-1096	NN	denotes	TNF-α
T2982	1097-1100	VB	denotes	was
T2983	1101-1109	VB	denotes	obtained
T2984	1110-1114	IN	denotes	from
T2985	1115-1125	NNP	denotes	Quantikine
T2986	1126-1127	NNP	denotes	R
T2987	1127-1128	CC	denotes	&
T2988	1128-1129	NNP	denotes	D
T2989	1130-1137	NNP	denotes	Systems
T2990	1138-1139	-LRB-	denotes	(
T2991	1139-1150	NNP	denotes	Minneapolis
T2992	1150-1151	-COMMA-	denotes	,
T2993	1152-1154	NN	denotes	MN
T2994	1154-1155	-RRB-	denotes	)
T2995	1155-1169	NN	denotes	. Tris-glycine
T2996	1170-1173	CC	denotes	and
T2997	1174-1177	NN	denotes	DNA
T2998	1178-1189	NN	denotes	retardation
T2999	1190-1194	NN	denotes	gels
T3000	1194-1195	-COMMA-	denotes	,
T3001	1196-1210	NN	denotes	nitrocellulose
T3002	1211-1220	NN	denotes	membranes
T3003	1220-1221	-COMMA-	denotes	,
T3004	1222-1225	CC	denotes	and
T3005	1226-1233	JJ	denotes	western
T3006	1234-1238	NN	denotes	blot
T3007	1239-1246	VB	denotes	running
T3008	1247-1254	NN	denotes	buffers
T3009	1255-1259	VB	denotes	were
T3010	1260-1268	VB	denotes	obtained
T3011	1269-1273	IN	denotes	from
T3012	1274-1284	NN	denotes	Invitrogen
T3013	1285-1286	-LRB-	denotes	(
T3014	1286-1294	NNP	denotes	Carlsbad
T3015	1294-1295	-COMMA-	denotes	,
T3016	1296-1298	NN	denotes	CA
T3017	1298-1299	-RRB-	denotes	)
T3018	1301-1316	JJ	denotes	NE-PER® nuclear
T3019	1317-1320	CC	denotes	and
T3020	1321-1332	JJ	denotes	cytoplasmic
T3021	1333-1343	NN	denotes	extraction
T3022	1344-1352	NN	denotes	reagents
T3023	1352-1353	-COMMA-	denotes	,
T3024	1354-1357	DT	denotes	the
T3025	1358-1361	NN	denotes	BCA
T3026	1362-1369	NN	denotes	protein
T3027	1370-1377	NN	denotes	reagent
T3028	1378-1381	NN	denotes	kit
T3029	1381-1382	-COMMA-	denotes	,
T3030	1383-1386	DT	denotes	the
T3031	1387-1398	NNP	denotes	Supersignal
T3032	1399-1403	NNP	denotes	West
T3033	1404-1408	NNP	denotes	Pico
T3034	1409-1425	NNP	denotes	Chemiluminescent
T3035	1426-1429	NNP	denotes	Kit
T3036	1429-1430	-COMMA-	denotes	,
T3037	1431-1434	CC	denotes	and
T3038	1435-1438	DT	denotes	the
T3039	1439-1449	NNP	denotes	LightShift
T3040	1450-1466	NNP	denotes	Chemiluminescent
T3041	1467-1471	NNP	denotes	EMSA
T3042	1472-1475	NN	denotes	Kit
T3043	1476-1480	VB	denotes	were
T3044	1481-1490	VB	denotes	purchased
T3045	1491-1495	IN	denotes	from
T3046	1496-1502	NNP	denotes	Pierce
T3047	1503-1504	-LRB-	denotes	(
T3048	1504-1512	NNP	denotes	Rockland
T3049	1512-1513	-COMMA-	denotes	,
T3050	1514-1516	NN	denotes	IL
T3051	1516-1517	-RRB-	denotes	)
T3052	1519-1523	NN	denotes	CREB
T3053	1524-1527	CC	denotes	and
T3054	1528-1533	NN	denotes	NF-κB
T3055	1534-1537	NN	denotes	DNA
T3056	1538-1553	NN	denotes	oligonucleotide
T3057	1554-1560	NN	denotes	probes
T3058	1561-1565	VB	denotes	were
T3059	1566-1577	VB	denotes	synthesized
T3060	1578-1580	IN	denotes	by
T3061	1581-1584	NN	denotes	IDT
T3062	1585-1586	-LRB-	denotes	(
T3063	1586-1596	NNP	denotes	Coralville
T3064	1596-1597	-COMMA-	denotes	,
T3065	1598-1600	NNP	denotes	IA
T3066	1600-1601	-RRB-	denotes	)
T3067	1603-1608	NN	denotes	Nylon
T3068	1609-1618	NN	denotes	membranes
T3069	1619-1623	VB	denotes	were
T3070	1624-1633	VB	denotes	purchased
T3071	1634-1638	IN	denotes	from
T3072	1639-1644	NNP	denotes	Roche
T3073	1645-1652	NNP	denotes	Applied
T3074	1653-1661	NNP	denotes	Sciences
T3075	1662-1663	-LRB-	denotes	(
T3076	1663-1675	NNP	denotes	Indianapolis
T3077	1675-1676	-COMMA-	denotes	,
T3078	1677-1679	NNP	denotes	IN
T3079	1679-1680	-RRB-	denotes	)
T3080	1682-1689	NN	denotes	Western
T3081	1690-1694	NN	denotes	blot
T3082	1695-1699	NN	denotes	band
T3083	1700-1714	NN	denotes	quantification
T3084	1715-1718	VB	denotes	was
T3085	1719-1728	VB	denotes	performed
T3086	1729-1733	IN	denotes	with
T3087	1734-1737	DT	denotes	the
T3088	1738-1748	NNP	denotes	UN-SCAN-IT
T3089	1749-1752	NNP	denotes	Gel
T3090	1753-1763	NNP	denotes	Digitizing
T3091	1764-1772	NN	denotes	software
T3092	1773-1774	-LRB-	denotes	(
T3093	1774-1778	NNP	denotes	Silk
T3094	1779-1789	NNP	denotes	Scientific
T3095	1789-1790	-COMMA-	denotes	,
T3096	1791-1795	NNP	denotes	Orem
T3097	1795-1796	-COMMA-	denotes	,
T3098	1797-1799	NN	denotes	UT
T3099	1799-1800	-RRB-	denotes	)
T3577	1803-1808	JJ	denotes	Human
T3578	1809-1819	NNP	denotes	Peripheral
T3579	1820-1825	NNP	denotes	Blood
T3580	1826-1837	NNP	denotes	Mononuclear
T3581	1838-1842	NNP	denotes	Cell
T3582	1843-1852	NN	denotes	Isolation
T3583	1853-1856	CC	denotes	and
T3584	1857-1868	NN	denotes	Stimulation
T3585	1869-1874	JJ	denotes	Human
T3586	1875-1886	JJ	denotes	mononuclear
T3587	1887-1892	NN	denotes	cells
T3588	1893-1897	VB	denotes	were
T3589	1898-1906	VB	denotes	isolated
T3590	1907-1911	IN	denotes	from
T3591	1912-1915	DT	denotes	the
T3592	1916-1926	JJ	denotes	peripheral
T3593	1927-1932	NN	denotes	blood
T3594	1933-1935	IN	denotes	of
T3595	1936-1940	CD	denotes	four
T3596	1941-1948	JJ	denotes	healthy
T3597	1949-1954	JJ	denotes	human
T3598	1955-1965	NN	denotes	volunteers
T3599	1967-1970	DT	denotes	The
T3600	1971-1977	NN	denotes	sample
T3601	1978-1982	NN	denotes	size
T3602	1983-1986	VB	denotes	was
T3603	1987-1993	VB	denotes	chosen
T3604	1994-1998	IN	denotes	from
T3605	1999-2002	PRP-DOLLAR-	denotes	our
T3606	2003-2013	RB	denotes	previously
T3607	2014-2023	VB	denotes	published
T3608	2024-2028	NN	denotes	work
T3609	2028-2029	-COMMA-	denotes	,
T3610	2030-2035	WDT	denotes	which
T3611	2036-2048	VB	denotes	demonstrated
T3612	2049-2057	JJ	denotes	reliable
T3613	2058-2068	NN	denotes	results.10
T3614	2069-2073	NN	denotes	Cell
T3615	2074-2083	NN	denotes	isolation
T3616	2084-2087	CC	denotes	and
T3617	2088-2091	DT	denotes	all
T3618	2092-2102	JJ	denotes	subsequent
T3619	2103-2114	NN	denotes	experiments
T3620	2115-2119	VB	denotes	were
T3621	2120-2129	VB	denotes	conducted
T3622	2130-2135	IN	denotes	under
T3623	2136-2143	JJ	denotes	sterile
T3624	2144-2147	CC	denotes	and
T3625	2148-2160	JJ	denotes	pyrogen-free
T3626	2161-2171	NN	denotes	conditions
T3627	2173-2178	NN	denotes	Blood
T3628	2179-2188	VB	denotes	collected
T3629	2189-2191	IN	denotes	in
T3630	2192-2199	NN	denotes	heparin
T3631	2200-2205	NN	denotes	tubes
T3632	2206-2209	VB	denotes	was
T3633	2210-2219	VB	denotes	incubated
T3634	2220-2224	IN	denotes	with
T3635	2225-2232	NN	denotes	Dextran
T3636	2233-2237	NN	denotes	T500
T3637	2238-2241	CC	denotes	and
T3638	2242-2245	DT	denotes	the
T3639	2246-2249	JJ	denotes	red
T3640	2250-2255	NN	denotes	cells
T3641	2256-2260	VB	denotes	were
T3642	2261-2271	VB	denotes	sedimented
T3643	2272-2275	IN	denotes	for
T3644	2276-2278	CD	denotes	40
T3645	2279-2286	NN	denotes	minutes
T3646	2287-2289	IN	denotes	at
T3647	2290-2294	NN	denotes	room
T3648	2295-2306	NN	denotes	temperature
T3649	2308-2311	DT	denotes	The
T3650	2312-2321	JJ	denotes	resultant
T3651	2322-2327	NN	denotes	serum
T3652	2328-2331	VB	denotes	was
T3653	2332-2336	RB	denotes	then
T3654	2337-2343	VB	denotes	washed
T3655	2344-2348	IN	denotes	with
T3656	2349-2353	NN	denotes	HBSS
T3657	2354-2357	CC	denotes	and
T3658	2358-2367	VB	denotes	separated
T3659	2368-2370	IN	denotes	by
T3660	2371-2378	NNP	denotes	Percoll
T3661	2379-2387	NN	denotes	gradient
T3662	2388-2402	NN	denotes	centrifugation
T3663	2403-2412	VB	denotes	according
T3664	2413-2415	TO	denotes	to
T3665	2416-2419	DT	denotes	the
T3666	2420-2434	NN	denotes	manufacturer’s
T3667	2435-2447	NN	denotes	instructions
T3668	2449-2453	NN	denotes	Cell
T3669	2454-2463	NN	denotes	viability
T3670	2464-2467	VB	denotes	was
T3671	2468-2476	VB	denotes	assessed
T3672	2477-2479	IN	denotes	by
T3673	2480-2486	NN	denotes	trypan
T3674	2487-2491	JJ	denotes	blue
T3675	2492-2495	NN	denotes	dye
T3676	2496-2505	NN	denotes	exclusion
T3677	2505-2506	-COMMA-	denotes	,
T3678	2507-2511	IN	denotes	with
T3679	2512-2518	NN	denotes	purity
T3680	2519-2521	IN	denotes	of
T3681	2522-2529	JJ	denotes	greater
T3682	2530-2534	IN	denotes	than
T3683	2535-2537	CD	denotes	95
T3684	2537-2538	NN	denotes	%
T3685	2540-2548	VB	denotes	Isolated
T3686	2549-2554	NN	denotes	cells
T3687	2555-2559	VB	denotes	were
T3688	2560-2571	VB	denotes	resuspended
T3689	2572-2574	IN	denotes	in
T3690	2575-2579	NN	denotes	RPMI
T3691	2580-2584	CD	denotes	1640
T3692	2585-2597	VB	denotes	supplemented
T3693	2598-2602	IN	denotes	with
T3694	2603-2605	CD	denotes	10
T3695	2605-2606	NN	denotes	%
T3696	2607-2610	NN	denotes	FBS
T3697	2611-2614	CC	denotes	and
T3698	2615-2616	CD	denotes	5
T3699	2617-2619	NN	denotes	mM
T3700	2620-2625	NN	denotes	HEPES
T3701	2626-2628	IN	denotes	at
T3702	2629-2630	DT	denotes	a
T3703	2631-2644	NN	denotes	concentration
T3704	2645-2647	IN	denotes	of
T3705	2648-2649	CD	denotes	1
T3706	2650-2651	SYM	denotes	x
T3707	2652-2663	NN	denotes	107cells/mL
T3708	2665-2669	DT	denotes	Each
T3709	2670-2680	JJ	denotes	subsequent
T3710	2681-2691	NN	denotes	experiment
T3711	2692-2698	VB	denotes	listed
T3712	2699-2704	IN	denotes	below
T3713	2705-2708	VB	denotes	was
T3714	2709-2718	VB	denotes	conducted
T3715	2719-2721	IN	denotes	on
T3716	2722-2729	NN	denotes	samples
T3717	2730-2732	IN	denotes	of
T3718	2733-2734	CD	denotes	5
T3719	2735-2736	NN	denotes	x
T3720	2737-2740	CD	denotes	106
T3721	2741-2746	NN	denotes	cells
T3722	2747-2750	IN	denotes	per
T3723	2751-2760	NN	denotes	treatment
T3724	2761-2766	NN	denotes	group
T3725	2768-2776	VB	denotes	Isolated
T3726	2777-2782	NN	denotes	cells
T3727	2783-2787	VB	denotes	were
T3728	2788-2798	VB	denotes	stimulated
T3729	2799-2803	IN	denotes	with
T3730	2804-2810	CC	denotes	either
T3731	2811-2815	NN	denotes	HBSS
T3732	2816-2818	IN	denotes	as
T3733	2819-2820	DT	denotes	a
T3734	2821-2829	JJ	denotes	negative
T3735	2830-2837	NN	denotes	control
T3736	2837-2838	-COMMA-	denotes	,
T3737	2839-2842	NN	denotes	LPS
T3738	2843-2844	-LRB-	denotes	(
T3739	2844-2845	CD	denotes	1
T3740	2846-2851	NN	denotes	μg/mL
T3741	2851-2852	-RRB-	denotes	)
T3742	2852-2853	-COMMA-	denotes	,
T3743	2854-2857	NN	denotes	PTX
T3744	2858-2859	-LRB-	denotes	(
T3745	2859-2861	CD	denotes	20
T3746	2862-2864	NN	denotes	mM
T3747	2864-2865	-RRB-	denotes	)
T3748	2865-2866	-COMMA-	denotes	,
T3749	2867-2869	CC	denotes	or
T3750	2870-2881	JJ	denotes	concomitant
T3751	2882-2885	NN	denotes	LPS
T3752	2886-2887	SYM	denotes	/
T3753	2888-2891	NN	denotes	PTX
T3754	2891-2892	-COMMA-	denotes	,
T3755	2893-2895	IN	denotes	at
T3756	2896-2899	DT	denotes	the
T3757	2900-2915	JJ	denotes	above-mentioned
T3758	2916-2930	NN	denotes	concentrations
T3759	2930-2931	-COMMA-	denotes	,
T3760	2932-2935	IN	denotes	for
T3761	2936-2938	CD	denotes	30
T3762	2939-2946	NN	denotes	minutes
T3763	2947-2949	IN	denotes	at
T3764	2950-2954	NN	denotes	37ºC
T3765	2956-2961	NN	denotes	Cells
T3766	2962-2966	VB	denotes	were
T3767	2967-2973	VB	denotes	placed
T3768	2974-2976	IN	denotes	on
T3769	2977-2980	NN	denotes	ice
T3770	2981-2984	IN	denotes	for
T3771	2985-2987	CD	denotes	10
T3772	2988-2995	NN	denotes	minutes
T3773	2996-2998	TO	denotes	to
T3774	2999-3003	VB	denotes	stop
T3775	3004-3007	DT	denotes	the
T3776	3008-3016	NN	denotes	reaction
T3777	3017-3020	CC	denotes	and
T3778	3021-3024	DT	denotes	the
T3779	3025-3032	NN	denotes	samples
T3780	3033-3037	VB	denotes	were
T3781	3038-3042	RB	denotes	then
T3782	3043-3049	VB	denotes	stored
T3783	3050-3052	IN	denotes	at
T3784	3053-3058	NN	denotes	−70°C
T3785	3059-3062	IN	denotes	for
T3786	3063-3070	JJ	denotes	further
T3787	3071-3079	NN	denotes	analysis
T3788	3081-3084	DT	denotes	The
T3789	3085-3096	NN	denotes	stimulation
T3790	3097-3102	NN	denotes	times
T3791	3103-3106	CC	denotes	and
T3792	3107-3121	NN	denotes	concentrations
T3793	3122-3124	IN	denotes	of
T3794	3125-3128	NN	denotes	LPS
T3795	3129-3132	CC	denotes	and
T3796	3133-3136	NN	denotes	PTX
T3797	3137-3145	VB	denotes	utilized
T3798	3146-3148	IN	denotes	in
T3799	3149-3153	DT	denotes	this
T3800	3154-3159	NN	denotes	study
T3801	3160-3164	VB	denotes	were
T3802	3165-3175	VB	denotes	determined
T3803	3176-3178	IN	denotes	in
T3804	3179-3187	JJ	denotes	previous
T3805	3188-3193	NN	denotes	pilot
T3806	3194-3201	NN	denotes	studies
T3807	3202-3206	VB	denotes	done
T3808	3207-3209	IN	denotes	in
T3809	3210-3213	PRP-DOLLAR-	denotes	our
T3810	3214-3224	NN	denotes	laboratory
T3811	3224-3225	-COMMA-	denotes	,
T3812	3226-3231	WDT	denotes	which
T3813	3232-3240	VB	denotes	examined
T3814	3241-3244	DT	denotes	the
T3815	3245-3252	NN	denotes	effects
T3816	3253-3255	IN	denotes	of
T3817	3256-3266	VB	denotes	increasing
T3818	3267-3281	NN	denotes	concentrations
T3819	3282-3284	IN	denotes	of
T3820	3285-3288	NN	denotes	PTX
T3821	3289-3291	IN	denotes	on
T3822	3292-3303	JJ	denotes	LPS-induced
T3823	3304-3309	NN	denotes	TNF-α
T3824	3310-3320	NN	denotes	production
T3825	3322-3325	NN	denotes	LPS
T3826	3326-3328	IN	denotes	at
T3827	3329-3330	DT	denotes	a
T3828	3331-3344	NN	denotes	concentration
T3829	3345-3347	IN	denotes	of
T3830	3348-3349	CD	denotes	1
T3831	3350-3355	NN	denotes	μg/mL
T3832	3356-3359	CC	denotes	and
T3833	3360-3363	NN	denotes	PTX
T3834	3364-3366	IN	denotes	at
T3835	3367-3368	DT	denotes	a
T3836	3369-3382	NN	denotes	concentration
T3837	3383-3385	IN	denotes	of
T3838	3386-3388	CD	denotes	20
T3839	3389-3391	NN	denotes	mM
T3840	3392-3396	VB	denotes	were
T3841	3397-3400	DT	denotes	the
T3842	3401-3408	JJ	denotes	minimum
T3843	3409-3423	NN	denotes	concentrations
T3844	3424-3433	JJ	denotes	necessary
T3845	3434-3436	TO	denotes	to
T3846	3437-3444	VB	denotes	produce
T3847	3445-3446	DT	denotes	a
T3848	3447-3459	JJ	denotes	reproducible
T3849	3460-3463	CC	denotes	and
T3850	3464-3472	JJ	denotes	reliable
T3851	3473-3486	NN	denotes	up-regulation
T3852	3487-3490	CC	denotes	and
T3853	3491-3506	NN	denotes	down-regulation
T3854	3507-3509	IN	denotes	in
T3855	3510-3515	NN	denotes	TNF-α
T3856	3516-3526	NN	denotes	production
T3857	3526-3527	-COMMA-	denotes	,
T3858	3528-3540	RB	denotes	respectively
T3859	3540-3541	-COMMA-	denotes	,
T3860	3542-3544	IN	denotes	in
T3861	3545-3557	JJ	denotes	quantitative
T3862	3558-3564	NN	denotes	assays
T3863	3565-3566	-LRB-	denotes	(
T3864	3566-3570	NN	denotes	data
T3865	3571-3574	RB	denotes	not
T3866	3575-3580	VB	denotes	shown
T3867	3580-3581	-RRB-	denotes	)
T4023	3584-3590	NN	denotes	Effect
T4024	3591-3593	IN	denotes	of
T4025	3594-3597	NN	denotes	PTX
T4027	3601-3612	JJ	denotes	LPS-induced
T4028	3613-3618	NN	denotes	TNF-α
T4029	3619-3629	NN	denotes	production
T4030	3630-3632	IN	denotes	by
T4031	3633-3644	JJ	denotes	mononuclear
T4032	3645-3650	NN	denotes	cells
T4033	3651-3654	DT	denotes	The
T4034	3655-3668	NN	denotes	concentration
T4035	3669-3671	IN	denotes	of
T4036	3672-3677	NN	denotes	TNF-α
T4037	3678-3681	VB	denotes	was
T4038	3682-3696	RB	denotes	quantitatively
T4039	3697-3707	VB	denotes	determined
T4040	3708-3710	IN	denotes	by
T4041	3711-3716	NN	denotes	ELISA
T4042	3717-3719	IN	denotes	in
T4043	3720-3723	DT	denotes	the
T4044	3724-3729	NN	denotes	media
T4045	3730-3732	IN	denotes	of
T4046	3733-3738	NN	denotes	cells
T4047	3739-3746	VB	denotes	exposed
T4049	3750-3753	DT	denotes	the
T4050	3754-3764	NN	denotes	treatments
T4051	3765-3774	VB	denotes	described
T4052	3775-3780	IN	denotes	above
T4053	3781-3786	IN	denotes	after
T4054	3787-3798	NN	denotes	stimulation
T4055	3799-3802	IN	denotes	for
T4056	3803-3805	RB	denotes	up
T4057	3806-3808	TO	denotes	to
T4058	3809-3810	DT	denotes	a
T4059	3811-3818	NN	denotes	maximum
T4060	3819-3821	IN	denotes	of
T4061	3822-3824	CD	denotes	18
T4062	3825-3830	NN	denotes	hours
T4063	3830-3831	-COMMA-	denotes	,
T4064	3832-3834	IN	denotes	at
T4065	3835-3841	JJ	denotes	3-hour
T4066	3842-3851	NN	denotes	intervals
T4067	3853-3856	DT	denotes	The
T4068	3857-3864	NN	denotes	results
T4069	3865-3868	VB	denotes	are
T4070	3869-3878	VB	denotes	expressed
T4071	3879-3881	IN	denotes	in
T4072	3882-3887	NN	denotes	pg/mL
T4475	3890-3898	NN	denotes	SDS-PAGE
T4476	3899-3902	CC	denotes	and
T4477	3903-3917	NN	denotes	Immunoblotting
T4478	3918-3919	-LRB-	denotes	(
T4479	3919-3924	NN	denotes	I-κBα
T4480	3924-3925	-COMMA-	denotes	,
T4481	3926-3931	NN	denotes	NF-κB
T4482	3932-3935	NN	denotes	p65
T4483	3935-3936	-COMMA-	denotes	,
T4484	3937-3940	CC	denotes	and
T4485	3941-3945	NN	denotes	CREB
T4486	3945-3946	-RRB-	denotes	)
T4487	3947-3956	VB	denotes	Following
T4488	3957-3968	NN	denotes	stimulation
T4489	3968-3969	-COMMA-	denotes	,
T4490	3970-3975	NN	denotes	PBMCs
T4491	3976-3980	VB	denotes	were
T4492	3981-3987	VB	denotes	washed
T4493	3988-3992	IN	denotes	with
T4494	3993-4001	JJ	denotes	ice-cold
T4495	4002-4005	NN	denotes	PBS
T4496	4006-4009	CC	denotes	and
T4497	4010-4021	VB	denotes	centrifuged
T4498	4022-4024	TO	denotes	to
T4499	4025-4032	VB	denotes	collect
T4500	4033-4034	DT	denotes	a
T4501	4035-4039	NN	denotes	cell
T4502	4040-4046	NN	denotes	pellet
T4503	4048-4051	DT	denotes	The
T4504	4052-4058	NN	denotes	pellet
T4505	4059-4062	VB	denotes	was
T4506	4063-4074	VB	denotes	resuspended
T4507	4075-4077	IN	denotes	in
T4508	4078-4086	JJ	denotes	ice-cold
T4509	4087-4090	NN	denotes	SDS
T4510	4091-4097	NN	denotes	sample
T4511	4098-4104	NN	denotes	buffer
T4512	4105-4117	VB	denotes	supplemented
T4513	4118-4122	IN	denotes	with
T4514	4123-4126	CD	denotes	100
T4515	4127-4129	NN	denotes	mM
T4516	4130-4144	NN	denotes	dithiothreitol
T4517	4146-4157	JJ	denotes	Cytoplasmic
T4518	4158-4161	CC	denotes	and
T4519	4162-4169	JJ	denotes	nuclear
T4520	4170-4178	NN	denotes	extracts
T4521	4179-4183	VB	denotes	were
T4522	4184-4192	VB	denotes	isolated
T4523	4193-4197	IN	denotes	with
T4524	4198-4204	NN	denotes	NE-PER
T4525	4205-4212	JJ	denotes	nuclear
T4526	4213-4216	CC	denotes	and
T4527	4217-4228	JJ	denotes	cytoplasmic
T4528	4229-4239	NN	denotes	extraction
T4529	4240-4248	NN	denotes	reagents
T4530	4249-4253	IN	denotes	with
T4531	4254-4256	NN	denotes	1x
T4532	4257-4261	NNP	denotes	Halt
T4533	4262-4270	NNP	denotes	Protease
T4534	4271-4280	NNP	denotes	Inhibitor
T4535	4281-4289	NNP	denotes	Cocktail
T4536	4290-4299	VB	denotes	according
T4537	4300-4302	TO	denotes	to
T4538	4303-4306	DT	denotes	the
T4539	4307-4321	NN	denotes	manufacturer’s
T4540	4322-4334	NN	denotes	instructions
T4541	4336-4343	NN	denotes	Protein
T4542	4344-4358	NN	denotes	concentrations
T4543	4359-4363	VB	denotes	were
T4544	4364-4374	VB	denotes	determined
T4545	4375-4379	IN	denotes	with
T4546	4380-4383	DT	denotes	the
T4547	4384-4387	NN	denotes	BCA
T4548	4388-4395	NN	denotes	protein
T4549	4396-4403	NN	denotes	reagent
T4550	4404-4407	NN	denotes	kit
T4551	4408-4411	IN	denotes	for
T4552	4412-4416	DT	denotes	each
T4553	4417-4423	NN	denotes	sample
T4554	4424-4433	VB	denotes	according
T4555	4434-4436	TO	denotes	to
T4556	4437-4438	DT	denotes	a
T4557	4439-4451	JJ	denotes	standardized
T4558	4452-4457	NN	denotes	curve
T4559	4458-4461	IN	denotes	for
T4560	4462-4469	NN	denotes	albumin
T4561	4471-4475	NN	denotes	Cell
T4562	4476-4483	NN	denotes	lysates
T4563	4484-4488	VB	denotes	were
T4564	4489-4498	VB	denotes	collected
T4565	4499-4501	IN	denotes	by
T4566	4502-4509	VB	denotes	boiling
T4567	4510-4513	DT	denotes	the
T4568	4514-4521	NN	denotes	samples
T4569	4522-4524	IN	denotes	in
T4570	4525-4526	DT	denotes	a
T4571	4527-4532	NN	denotes	100ºC
T4572	4533-4538	NN	denotes	water
T4573	4539-4543	NN	denotes	bath
T4574	4544-4547	IN	denotes	for
T4575	4548-4549	CD	denotes	5
T4576	4550-4557	NN	denotes	minutes
T4577	4559-4562	CD	denotes	Ten
T4578	4563-4565	NN	denotes	μg
T4579	4566-4568	IN	denotes	of
T4580	4569-4576	NN	denotes	protein
T4581	4577-4580	IN	denotes	per
T4582	4581-4587	NN	denotes	sample
T4583	4588-4591	VB	denotes	was
T4584	4592-4601	VB	denotes	separated
T4585	4602-4604	IN	denotes	by
T4586	4605-4623	NN	denotes	SDS-polyacrylamide
T4587	4624-4627	NN	denotes	gel
T4588	4628-4643	NN	denotes	electrophoresis
T4589	4644-4651	IN	denotes	through
T4590	4652-4656	CD	denotes	8–16
T4591	4656-4657	NN	denotes	%
T4592	4658-4670	NN	denotes	tris-glycine
T4593	4671-4685	NN	denotes	polyacrylamide
T4594	4686-4694	NN	denotes	gradient
T4595	4695-4699	NN	denotes	gels
T4596	4700-4703	CC	denotes	and
T4597	4704-4715	VB	denotes	transferred
T4598	4716-4718	TO	denotes	to
T4599	4719-4733	VB	denotes	nitrocellulose
T4600	4734-4743	NN	denotes	membranes
T4601	4745-4748	DT	denotes	The
T4602	4749-4758	NN	denotes	membranes
T4603	4759-4763	VB	denotes	were
T4604	4764-4771	VB	denotes	blocked
T4605	4772-4776	IN	denotes	with
T4606	4777-4778	CD	denotes	5
T4607	4778-4779	NN	denotes	%
T4608	4780-4784	NN	denotes	milk
T4609	4785-4787	IN	denotes	in
T4610	4788-4801	JJ	denotes	Tris-buffered
T4611	4802-4814	NN	denotes	saline/Tween
T4612	4815-4817	CD	denotes	20
T4613	4818-4819	-LRB-	denotes	(
T4614	4819-4826	NNP	denotes	Fischer
T4615	4827-4837	NNP	denotes	Scientific
T4616	4837-4838	-COMMA-	denotes	,
T4617	4839-4849	NNP	denotes	Pittsburgh
T4618	4849-4850	-COMMA-	denotes	,
T4619	4851-4853	NNP	denotes	PA
T4620	4853-4854	-RRB-	denotes	)
T4621	4855-4858	IN	denotes	for
T4622	4859-4860	CD	denotes	1
T4623	4861-4865	NN	denotes	hour
T4624	4867-4878	JJ	denotes	Cytoplasmic
T4625	4879-4887	NN	denotes	extracts
T4626	4888-4892	VB	denotes	were
T4627	4893-4902	VB	denotes	incubated
T4628	4903-4907	IN	denotes	with
T4629	4908-4922	VB	denotes	phosphorylated
T4630	4923-4928	JJ	denotes	I-κBα
T4631	4929-4937	NN	denotes	antibody
T4632	4938-4939	-LRB-	denotes	(
T4633	4939-4940	CD	denotes	1
T4634	4940-4941	-COLON-	denotes	:
T4635	4941-4944	CD	denotes	200
T4636	4944-4945	-RRB-	denotes	)
T4637	4945-4946	-COMMA-	denotes	,
T4638	4947-4952	IN	denotes	while
T4639	4953-4960	JJ	denotes	nuclear
T4640	4961-4969	NN	denotes	extracts
T4641	4970-4974	VB	denotes	were
T4642	4975-4984	VB	denotes	incubated
T4643	4985-4989	IN	denotes	with
T4644	4990-4996	CC	denotes	either
T4645	4997-5011	VB	denotes	phosphorylated
T4646	5012-5017	NN	denotes	NF-κB
T4647	5018-5021	NN	denotes	p65
T4648	5022-5030	NN	denotes	antibody
T4649	5031-5032	-LRB-	denotes	(
T4650	5032-5033	CD	denotes	1
T4651	5033-5034	-COLON-	denotes	:
T4652	5034-5037	CD	denotes	500
T4653	5037-5038	-RRB-	denotes	)
T4654	5039-5041	CC	denotes	or
T4655	5042-5056	VB	denotes	phosphorylated
T4656	5057-5061	NN	denotes	CREB
T4657	5062-5070	NN	denotes	antibody
T4658	5071-5072	-LRB-	denotes	(
T4659	5072-5073	CD	denotes	1
T4660	5073-5074	-COLON-	denotes	:
T4661	5074-5077	CD	denotes	500
T4662	5077-5078	-RRB-	denotes	)
T4663	5079-5088	RB	denotes	overnight
T4664	5089-5091	IN	denotes	at
T4665	5092-5095	NN	denotes	4°C
T4666	5096-5098	IN	denotes	in
T4667	5099-5107	JJ	denotes	separate
T4668	5108-5119	NN	denotes	experiments
T4669	5121-5124	DT	denotes	The
T4670	5125-5134	NN	denotes	membranes
T4671	5135-5139	VB	denotes	were
T4672	5140-5146	VB	denotes	washed
T4673	5147-5151	IN	denotes	with
T4674	5152-5165	JJ	denotes	Tris-buffered
T4675	5166-5178	NN	denotes	saline/Tween
T4676	5179-5181	CD	denotes	20
T4677	5182-5185	CC	denotes	and
T4678	5186-5195	VB	denotes	incubated
T4679	5196-5199	IN	denotes	for
T4680	5200-5201	CD	denotes	1
T4681	5202-5206	NN	denotes	hour
T4682	5207-5209	IN	denotes	at
T4683	5210-5214	NN	denotes	room
T4684	5215-5226	NN	denotes	temperature
T4685	5227-5231	IN	denotes	with
T4686	5232-5235	DT	denotes	the
T4687	5236-5245	JJ	denotes	secondary
T4688	5246-5254	NN	denotes	antibody
T4689	5254-5255	-COMMA-	denotes	,
T4690	5256-5267	JJ	denotes	horseradish
T4691	5268-5285	JJ	denotes	peroxidase-linked
T4692	5286-5297	JJ	denotes	anti-rabbit
T4693	5298-5301	NN	denotes	IgG
T4694	5302-5309	VB	denotes	diluted
T4695	5310-5311	CD	denotes	1
T4696	5311-5312	-COLON-	denotes	:
T4697	5312-5316	CD	denotes	2000
T4698	5317-5319	IN	denotes	in
T4699	5320-5328	VB	denotes	blocking
T4700	5329-5337	NN	denotes	solution
T4701	5339-5344	IN	denotes	After
T4702	5345-5353	VB	denotes	repeated
T4703	5354-5361	NN	denotes	washing
T4704	5362-5364	IN	denotes	of
T4705	5365-5368	DT	denotes	the
T4706	5369-5377	NN	denotes	membrane
T4707	5377-5378	-COMMA-	denotes	,
T4708	5379-5382	DT	denotes	the
T4709	5383-5394	NNP	denotes	Supersignal
T4710	5395-5399	NNP	denotes	West
T4711	5400-5404	NNP	denotes	Pico
T4712	5405-5421	NNP	denotes	Chemiluminescent
T4713	5422-5425	NNP	denotes	Kit
T4714	5426-5429	VB	denotes	was
T4715	5430-5437	VB	denotes	applied
T4716	5438-5441	IN	denotes	for
T4717	5442-5450	NN	denotes	antibody
T4718	5451-5460	NN	denotes	detection
T4719	5461-5464	IN	denotes	per
T4720	5465-5468	DT	denotes	the
T4721	5469-5483	NN	denotes	manufacturer’s
T4722	5484-5496	NN	denotes	instructions
T4723	5498-5505	JJ	denotes	Western
T4724	5506-5510	NN	denotes	blot
T4725	5511-5515	NN	denotes	data
T4726	5516-5518	VB	denotes	is
T4727	5519-5528	VB	denotes	presented
T4728	5529-5531	IN	denotes	as
T4729	5532-5533	DT	denotes	a
T4730	5534-5544	NN	denotes	percentage
T4731	5545-5547	IN	denotes	of
T4732	5548-5551	NN	denotes	LPS
T4733	5552-5563	NN	denotes	stimulation
T4734	5565-5568	DT	denotes	The
T4735	5569-5579	NN	denotes	percentage
T4736	5580-5582	IN	denotes	of
T4737	5583-5586	NN	denotes	LPS
T4738	5587-5598	NN	denotes	stimulation
T4739	5599-5602	VB	denotes	was
T4740	5603-5613	VB	denotes	calculated
T4741	5614-5616	IN	denotes	by
T4742	5617-5625	VB	denotes	dividing
T4743	5626-5629	DT	denotes	the
T4744	5630-5634	JJ	denotes	mean
T4745	5635-5639	NN	denotes	band
T4746	5640-5645	NN	denotes	pixel
T4747	5646-5651	NN	denotes	total
T4748	5652-5655	IN	denotes	for
T4749	5656-5660	DT	denotes	each
T4750	5661-5670	NN	denotes	treatment
T4751	5671-5674	NN	denotes	arm
T4752	5675-5682	VB	denotes	divided
T4753	5683-5685	IN	denotes	by
T4754	5686-5689	DT	denotes	the
T4755	5690-5694	JJ	denotes	mean
T4756	5695-5700	NN	denotes	pixel
T4757	5701-5706	NN	denotes	total
T4758	5707-5709	IN	denotes	of
T4759	5710-5717	NN	denotes	samples
T4760	5718-5728	VB	denotes	stimulated
T4761	5729-5733	IN	denotes	with
T4762	5734-5737	NN	denotes	LPS
T4763	5738-5741	CC	denotes	and
T4764	5742-5753	VB	denotes	multiplying
T4765	5754-5756	IN	denotes	by
T4766	5757-5760	CD	denotes	100
T4767	5762-5766	RB	denotes	Thus
T4768	5766-5767	-COMMA-	denotes	,
T4769	5768-5771	NN	denotes	LPS
T4770	5772-5783	NN	denotes	stimulation
T4771	5784-5786	VB	denotes	is
T4772	5787-5795	VB	denotes	reported
T4773	5796-5798	IN	denotes	as
T4774	5799-5802	CD	denotes	100
T4775	5802-5803	NN	denotes	%
T4776	5804-5807	CC	denotes	and
T4777	5808-5812	DT	denotes	each
T4778	5813-5815	IN	denotes	of
T4779	5816-5819	DT	denotes	the
T4780	5820-5829	NN	denotes	treatment
T4781	5830-5834	NN	denotes	arms
T4782	5835-5837	VB	denotes	is
T4783	5838-5846	VB	denotes	reported
T4784	5847-5849	IN	denotes	as
T4785	5850-5851	DT	denotes	a
T4786	5852-5859	NN	denotes	percent
T4787	5860-5862	IN	denotes	of
T4788	5863-5866	NN	denotes	LPS
T4789	5867-5878	NN	denotes	stimulation
T5231	5881-5886	NN	denotes	NF-κB
T5232	5887-5890	CC	denotes	and
T5233	5891-5895	NN	denotes	CREB
T5234	5896-5911	JJ	denotes	Electrophoretic
T5235	5912-5920	NN	denotes	Mobility
T5236	5921-5926	NN	denotes	Shift
T5237	5927-5932	NN	denotes	Assay
T5238	5933-5934	-LRB-	denotes	(
T5239	5934-5938	NN	denotes	EMSA
T5240	5938-5939	-RRB-	denotes	)
T5241	5940-5943	DT	denotes	The
T5242	5944-5959	JJ	denotes	non-radioactive
T5243	5960-5970	NNP	denotes	LightShift
T5244	5971-5987	NNP	denotes	Chemiluminescent
T5245	5988-5992	NNP	denotes	EMSA
T5246	5993-5996	NN	denotes	Kit
T5247	5997-6000	VB	denotes	was
T5248	6001-6005	VB	denotes	used
T5249	6006-6008	TO	denotes	to
T5250	6009-6015	VB	denotes	detect
T5251	6016-6027	JJ	denotes	DNA-protein
T5252	6028-6040	NN	denotes	interactions
T5253	6042-6045	DT	denotes	The
T5254	6046-6051	NN	denotes	NF-κB
T5255	6052-6054	NN	denotes	3′
T5256	6055-6061	NN	denotes	biotin
T5257	6062-6073	JJ	denotes	end-labeled
T5258	6074-6077	NN	denotes	DNA
T5259	6078-6093	NN	denotes	oligonucleotide
T5260	6094-6098	VB	denotes	used
T5261	6099-6101	IN	denotes	as
T5262	6102-6103	DT	denotes	a
T5263	6104-6109	NN	denotes	probe
T5264	6110-6113	IN	denotes	for
T5265	6114-6117	DT	denotes	the
T5266	6118-6122	NN	denotes	EMSA
T5267	6123-6126	VB	denotes	was
T5268	6127-6128	DT	denotes	a
T5269	6129-6134	JJ	denotes	42-bp
T5270	6135-6141	JJ	denotes	double
T5271	6142-6150	JJ	denotes	stranded
T5272	6151-6160	NN	denotes	construct
T5273	6161-6162	-LRB-	denotes	(
T5274	6162-6208	NN	denotes	5′-TTGTTACAA-GGGGACTTTCCGCTGGGGACTTTCCAGGGAGGC
T5275	6209-6210	NN	denotes	–
T5276	6211-6213	NN	denotes	3′
T5277	6213-6214	-RRB-	denotes	)
T5278	6215-6225	VB	denotes	containing
T5279	6226-6229	CD	denotes	two
T5280	6230-6238	RB	denotes	tandemly
T5281	6239-6247	VB	denotes	repeated
T5282	6248-6253	NN	denotes	NF-κB
T5283	6254-6261	NN	denotes	binding
T5284	6262-6267	NN	denotes	sites
T5285	6268-6269	-LRB-	denotes	(
T5286	6269-6279	VB	denotes	underlined
T5287	6279-6280	-RRB-	denotes	)
T5288	6282-6293	NN	denotes	Specificity
T5289	6294-6297	VB	denotes	was
T5290	6298-6308	VB	denotes	determined
T5291	6309-6311	IN	denotes	by
T5292	6312-6313	DT	denotes	a
T5293	6314-6325	NN	denotes	competition
T5294	6326-6331	NN	denotes	assay
T5295	6332-6336	IN	denotes	with
T5296	6337-6340	DT	denotes	the
T5297	6341-6349	NN	denotes	addition
T5298	6350-6352	IN	denotes	of
T5299	6353-6356	CD	denotes	200
T5300	6357-6362	JJ	denotes	molar
T5301	6363-6369	NN	denotes	excess
T5302	6370-6372	IN	denotes	of
T5303	6373-6382	JJ	denotes	unlabeled
T5304	6383-6389	JJ	denotes	double
T5305	6390-6398	JJ	denotes	stranded
T5306	6399-6404	NN	denotes	NF-κB
T5307	6405-6420	NN	denotes	oligonucleotide
T5308	6422-6425	DT	denotes	The
T5309	6426-6430	NN	denotes	CREB
T5310	6431-6433	NN	denotes	3′
T5311	6434-6440	NN	denotes	biotin
T5312	6441-6452	JJ	denotes	end-labeled
T5313	6453-6456	NN	denotes	DNA
T5314	6457-6472	NN	denotes	oligonucleotide
T5315	6473-6477	VB	denotes	used
T5316	6478-6480	IN	denotes	as
T5317	6481-6482	DT	denotes	a
T5318	6483-6488	NN	denotes	probe
T5319	6489-6492	IN	denotes	for
T5320	6493-6496	DT	denotes	the
T5321	6497-6501	NN	denotes	EMSA
T5322	6502-6505	VB	denotes	was
T5323	6506-6507	DT	denotes	a
T5324	6508-6513	JJ	denotes	23-bp
T5325	6514-6520	JJ	denotes	double
T5326	6521-6529	JJ	denotes	stranded
T5327	6530-6539	NN	denotes	construct
T5328	6540-6541	-LRB-	denotes	(
T5329	6541-6547	NN	denotes	5′-TTT
T5330	6548-6551	NN	denotes	TCG
T5331	6552-6555	NN	denotes	AGC
T5332	6556-6571	NN	denotes	TCTGACGTCAGA-GC
T5333	6572-6573	NN	denotes	–
T5334	6574-6576	NN	denotes	3′
T5335	6576-6577	-RRB-	denotes	)
T5336	6578-6588	VB	denotes	containing
T5337	6589-6592	DT	denotes	the
T5338	6593-6596	NN	denotes	CRE
T5339	6597-6606	NN	denotes	consensus
T5340	6607-6615	NN	denotes	sequence
T5341	6616-6617	-LRB-	denotes	(
T5342	6617-6627	VB	denotes	underlined
T5343	6627-6628	-RRB-	denotes	)
T5344	6630-6641	NN	denotes	Specificity
T5345	6642-6645	VB	denotes	was
T5346	6646-6656	VB	denotes	determined
T5347	6657-6659	IN	denotes	by
T5348	6660-6661	DT	denotes	a
T5349	6662-6673	NN	denotes	competition
T5350	6674-6679	NN	denotes	assay
T5351	6680-6684	IN	denotes	with
T5352	6685-6688	DT	denotes	the
T5353	6689-6697	NN	denotes	addition
T5354	6698-6700	IN	denotes	of
T5355	6701-6704	CD	denotes	200
T5356	6705-6710	JJ	denotes	molar
T5357	6711-6717	NN	denotes	excess
T5358	6718-6720	IN	denotes	of
T5359	6721-6730	JJ	denotes	unlabeled
T5360	6731-6737	JJ	denotes	double
T5361	6738-6746	JJ	denotes	stranded
T5362	6747-6751	NN	denotes	CREB
T5363	6752-6767	NN	denotes	oligonucleotide
T5364	6769-6776	JJ	denotes	Nuclear
T5365	6777-6785	NN	denotes	extracts
T5366	6786-6787	-LRB-	denotes	(
T5367	6787-6789	CD	denotes	10
T5368	6790-6792	NN	denotes	μg
T5369	6792-6793	-RRB-	denotes	)
T5370	6794-6798	VB	denotes	were
T5371	6799-6808	VB	denotes	incubated
T5372	6809-6813	IN	denotes	with
T5373	6814-6815	CD	denotes	5
T5374	6816-6818	NN	denotes	nM
T5375	6819-6824	NN	denotes	NF-κB
T5376	6825-6827	CC	denotes	or
T5377	6828-6832	NN	denotes	CREB
T5378	6833-6838	NN	denotes	probe
T5379	6839-6840	-LRB-	denotes	(
T5380	6840-6845	NN	denotes	NF-kB
T5381	6845-6846	-COLON-	denotes	:
T5382	6847-6849	NN	denotes	1x
T5383	6850-6857	NN	denotes	binding
T5384	6858-6864	NN	denotes	buffer
T5385	6864-6865	-COMMA-	denotes	,
T5386	6866-6868	CD	denotes	50
T5387	6869-6871	NN	denotes	mM
T5388	6872-6875	NN	denotes	KCl
T5389	6875-6876	-COMMA-	denotes	,
T5390	6877-6878	CD	denotes	1
T5391	6879-6881	NN	denotes	mM
T5392	6882-6886	NN	denotes	EDTA
T5393	6886-6887	-COMMA-	denotes	,
T5394	6888-6889	CD	denotes	1
T5395	6890-6892	NN	denotes	mM
T5396	6893-6896	NN	denotes	DTT
T5397	6896-6897	-COMMA-	denotes	,
T5398	6898-6901	CD	denotes	0.1
T5399	6901-6902	NN	denotes	%
T5400	6903-6907	NN	denotes	NP40
T5401	6907-6908	-COMMA-	denotes	,
T5402	6909-6911	CD	denotes	10
T5403	6911-6912	NN	denotes	%
T5404	6913-6921	NN	denotes	glycerol
T5405	6921-6922	-COMMA-	denotes	,
T5406	6923-6926	CC	denotes	and
T5407	6927-6929	CD	denotes	50
T5408	6930-6935	NN	denotes	ng/μl
T5409	6936-6940	NN	denotes	poly
T5410	6941-6946	NN	denotes	dI-dC
T5411	6946-6947	-COMMA-	denotes	,
T5412	6948-6952	NN	denotes	CREB
T5413	6952-6953	-COLON-	denotes	:
T5414	6954-6956	NN	denotes	1x
T5415	6957-6964	NN	denotes	binding
T5416	6965-6971	NN	denotes	buffer
T5417	6971-6972	-COMMA-	denotes	,
T5418	6973-6975	CD	denotes	20
T5419	6976-6978	NN	denotes	mM
T5420	6979-6983	NN	denotes	Tris
T5421	6983-6984	-COMMA-	denotes	,
T5422	6985-6987	NN	denotes	pH
T5423	6988-6991	CD	denotes	7.5
T5424	6991-6992	-COMMA-	denotes	,
T5425	6993-6995	CD	denotes	50
T5426	6996-6998	NN	denotes	mM
T5427	6999-7002	NN	denotes	KCl
T5428	7002-7003	-COMMA-	denotes	,
T5429	7004-7005	CD	denotes	1
T5430	7006-7008	NN	denotes	mM
T5431	7009-7013	NN	denotes	EDTA
T5432	7013-7014	-COMMA-	denotes	,
T5433	7015-7016	CD	denotes	1
T5434	7017-7019	NN	denotes	mM
T5435	7020-7023	NN	denotes	DTT
T5436	7023-7024	-COMMA-	denotes	,
T5437	7025-7029	CD	denotes	0.10
T5438	7029-7030	NN	denotes	%
T5439	7031-7035	NN	denotes	NP40
T5440	7035-7036	-COMMA-	denotes	,
T5441	7037-7038	CD	denotes	6
T5442	7038-7039	NN	denotes	%
T5443	7040-7048	NN	denotes	glycerol
T5444	7048-7049	-COMMA-	denotes	,
T5445	7050-7053	CD	denotes	0.1
T5446	7054-7059	NN	denotes	mg/mL
T5447	7060-7063	NN	denotes	BSA
T5448	7064-7067	CC	denotes	and
T5449	7068-7070	CD	denotes	50
T5450	7071-7076	NN	denotes	ng/μl
T5451	7077-7081	NN	denotes	poly
T5452	7082-7087	NN	denotes	dI-dC
T5453	7087-7088	-RRB-	denotes	)
T5454	7089-7092	CC	denotes	and
T5455	7093-7097	VB	denotes	were
T5456	7098-7102	RB	denotes	then
T5457	7103-7118	VB	denotes	electrophoresed
T5458	7119-7126	IN	denotes	through
T5459	7127-7128	DT	denotes	a
T5460	7129-7130	CD	denotes	6
T5461	7130-7131	NN	denotes	%
T5462	7132-7135	NN	denotes	DNA
T5463	7136-7147	NN	denotes	retardation
T5464	7148-7151	NN	denotes	gel
T5465	7152-7154	IN	denotes	at
T5466	7155-7159	NN	denotes	100V
T5467	7160-7163	IN	denotes	for
T5468	7164-7166	CD	denotes	90
T5469	7167-7174	NN	denotes	minutes
T5470	7176-7179	DT	denotes	The
T5471	7180-7184	NN	denotes	gels
T5472	7185-7189	VB	denotes	were
T5473	7190-7209	RB	denotes	electrophoretically
T5474	7210-7221	VB	denotes	transferred
T5475	7222-7224	IN	denotes	at
T5476	7225-7230	NN	denotes	380mA
T5477	7231-7234	IN	denotes	for
T5478	7235-7236	CD	denotes	1
T5479	7237-7241	NN	denotes	hour
T5480	7242-7244	IN	denotes	on
T5481	7245-7248	NN	denotes	ice
T5482	7249-7251	TO	denotes	to
T5483	7252-7253	DT	denotes	a
T5484	7254-7264	RB	denotes	positively
T5485	7265-7272	VB	denotes	charged
T5486	7273-7278	NN	denotes	nylon
T5487	7279-7287	NN	denotes	membrane
T5488	7288-7291	CC	denotes	and
T5489	7292-7303	RB	denotes	immediately
T5490	7304-7316	JJ	denotes	cross-linked
T5491	7317-7320	IN	denotes	for
T5492	7321-7323	CD	denotes	15
T5493	7324-7331	NN	denotes	minutes
T5494	7332-7336	IN	denotes	with
T5495	7337-7338	DT	denotes	a
T5496	7339-7341	NN	denotes	UV
T5497	7342-7358	NN	denotes	transilluminator
T5498	7359-7367	VB	denotes	equipped
T5499	7368-7372	IN	denotes	with
T5500	7373-7374	DT	denotes	a
T5501	7375-7378	CD	denotes	312
T5502	7379-7381	NN	denotes	nm
T5503	7382-7386	NN	denotes	bulb
T5504	7388-7412	JJ	denotes	Streptavidin-horseradish
T5505	7413-7423	NN	denotes	peroxidase
T5506	7424-7433	NN	denotes	conjugate
T5507	7434-7437	CC	denotes	and
T5508	7438-7441	DT	denotes	the
T5509	7442-7452	NNP	denotes	LightShift
T5510	7453-7469	NNP	denotes	Chemiluminescent
T5511	7470-7479	NNP	denotes	Substrate
T5512	7480-7484	VB	denotes	were
T5513	7485-7489	VB	denotes	used
T5514	7490-7492	TO	denotes	to
T5515	7493-7499	VB	denotes	detect
T5516	7500-7503	DT	denotes	the
T5517	7504-7510	NN	denotes	biotin
T5518	7511-7522	JJ	denotes	end-labeled
T5519	7523-7526	NN	denotes	DNA
T5520	7528-7531	DT	denotes	The
T5521	7532-7537	NN	denotes	nylon
T5522	7538-7547	NN	denotes	membranes
T5523	7548-7552	VB	denotes	were
T5524	7553-7560	VB	denotes	exposed
T5525	7561-7563	TO	denotes	to
T5526	7564-7569	NN	denotes	x-ray
T5527	7570-7574	NN	denotes	film
T5528	7575-7578	IN	denotes	for
T5529	7579-7582	CD	denotes	1–3
T5530	7583-7590	NN	denotes	minutes
T5531	7591-7594	IN	denotes	for
T5532	7595-7604	NN	denotes	detection
T5799	7607-7611	NN	denotes	Role
T5800	7612-7614	IN	denotes	of
T5801	7615-7618	NN	denotes	PKA
T5802	7619-7621	IN	denotes	on
T5803	7622-7633	JJ	denotes	LPS-induced
T5804	7634-7645	JJ	denotes	mononuclear
T5805	7646-7650	NN	denotes	cell
T5806	7651-7656	NN	denotes	TNF-α
T5807	7657-7667	NN	denotes	production
T5808	7668-7670	TO	denotes	To
T5809	7671-7679	VB	denotes	evaluate
T5810	7680-7683	DT	denotes	the
T5811	7684-7688	NN	denotes	role
T5812	7689-7691	IN	denotes	of
T5813	7692-7695	NN	denotes	PKA
T5814	7696-7698	IN	denotes	on
T5815	7699-7704	NN	denotes	TNF-α
T5816	7705-7715	NN	denotes	production
T5817	7716-7718	IN	denotes	in
T5818	7719-7730	JJ	denotes	mononuclear
T5819	7731-7736	NN	denotes	cells
T5820	7737-7740	CC	denotes	and
T5821	7741-7744	PRP-DOLLAR-	denotes	its
T5822	7745-7756	NN	denotes	involvement
T5823	7757-7759	IN	denotes	in
T5824	7760-7763	DT	denotes	the
T5825	7764-7775	NN	denotes	attenuation
T5826	7776-7778	IN	denotes	of
T5827	7779-7790	JJ	denotes	LPS-induced
T5828	7791-7796	NN	denotes	TNF-α
T5829	7797-7807	NN	denotes	production
T5830	7808-7816	VB	denotes	observed
T5831	7817-7826	VB	denotes	following
T5832	7827-7830	NN	denotes	PTX
T5833	7831-7840	NN	denotes	treatment
T5834	7840-7841	-COMMA-	denotes	,
T5835	7842-7845	NN	denotes	H89
T5836	7845-7846	-COMMA-	denotes	,
T5837	7847-7848	DT	denotes	a
T5838	7849-7857	JJ	denotes	specific
T5839	7858-7861	NN	denotes	PKA
T5840	7862-7871	NN	denotes	inhibitor
T5841	7871-7872	-COMMA-	denotes	,
T5842	7873-7876	VB	denotes	was
T5843	7877-7885	VB	denotes	utilized
T5844	7887-7895	VB	denotes	Isolated
T5845	7896-7907	JJ	denotes	mononuclear
T5846	7908-7913	NN	denotes	cells
T5847	7914-7918	VB	denotes	were
T5848	7919-7928	VB	denotes	incubated
T5849	7929-7938	VB	denotes	according
T5850	7939-7941	TO	denotes	to
T5851	7942-7945	DT	denotes	the
T5852	7946-7955	NN	denotes	treatment
T5853	7956-7962	NN	denotes	groups
T5854	7963-7972	VB	denotes	described
T5855	7973-7978	RB	denotes	above
T5856	7979-7981	IN	denotes	in
T5857	7982-7985	DT	denotes	the
T5858	7986-7994	NN	denotes	presence
T5859	7995-7998	CC	denotes	and
T5860	7999-8006	NN	denotes	absence
T5861	8007-8009	IN	denotes	of
T5862	8010-8022	NN	denotes	pretreatment
T5863	8023-8027	IN	denotes	with
T5864	8028-8031	NN	denotes	H89
T5865	8032-8033	-LRB-	denotes	(
T5866	8033-8035	CD	denotes	10
T5867	8036-8038	NN	denotes	μM
T5868	8038-8039	-RRB-	denotes	)
T5869	8040-8043	IN	denotes	for
T5870	8044-8045	CD	denotes	1
T5871	8046-8050	NN	denotes	hour
T5872	8051-8053	IN	denotes	at
T5873	8054-8058	NN	denotes	37ºC
T5874	8060-8063	DT	denotes	The
T5875	8064-8068	NN	denotes	dose
T5876	8069-8071	IN	denotes	of
T5877	8072-8075	NN	denotes	H89
T5878	8076-8079	VB	denotes	was
T5879	8080-8086	VB	denotes	chosen
T5880	8087-8092	VB	denotes	based
T5881	8093-8095	IN	denotes	on
T5882	8096-8104	JJ	denotes	previous
T5883	8105-8109	NN	denotes	work
T5884	8110-8115	WDT	denotes	which
T5885	8116-8128	VB	denotes	demonstrated
T5886	8129-8137	JJ	denotes	specific
T5887	8138-8141	CC	denotes	and
T5888	8142-8150	JJ	denotes	complete
T5889	8151-8161	NN	denotes	inhibition
T5890	8162-8164	IN	denotes	of
T5891	8165-8168	NN	denotes	PKA
T5892	8169-8171	IN	denotes	at
T5893	8172-8176	DT	denotes	this
T5894	8177-8193	NN	denotes	concentration.10
T5895	8194-8199	IN	denotes	Since
T5896	8200-8203	DT	denotes	the
T5897	8204-8212	NN	denotes	activity
T5898	8213-8215	IN	denotes	of
T5899	8216-8220	DT	denotes	this
T5900	8221-8230	NN	denotes	inhibitor
T5901	8231-8233	VB	denotes	is
T5902	8234-8238	CC	denotes	both
T5903	8239-8247	JJ	denotes	specific
T5904	8248-8251	CC	denotes	and
T5905	8252-8262	JJ	denotes	consistent
T5906	8263-8265	IN	denotes	at
T5907	8266-8270	DT	denotes	this
T5908	8271-8280	VB	denotes	specified
T5909	8281-8285	NN	denotes	dose
T5910	8285-8286	-COMMA-	denotes	,
T5911	8287-8289	PRP	denotes	we
T5912	8290-8293	VB	denotes	did
T5913	8294-8297	RB	denotes	not
T5914	8298-8305	VB	denotes	include
T5915	8306-8317	NN	denotes	experiments
T5916	8318-8322	IN	denotes	with
T5917	8323-8333	JJ	denotes	additional
T5918	8334-8337	NN	denotes	PKA
T5919	8338-8348	NN	denotes	inhibitors
T5920	8349-8353	IN	denotes	with
T5921	8354-8358	DT	denotes	this
T5922	8359-8362	NN	denotes	set
T5923	8363-8365	IN	denotes	of
T5924	8366-8377	NN	denotes	experiments
T5925	8379-8382	DT	denotes	The
T5926	8383-8388	NN	denotes	TNF-α
T5927	8389-8402	NN	denotes	concentration
T5928	8403-8405	IN	denotes	in
T5929	8406-8409	DT	denotes	the
T5930	8410-8421	NN	denotes	supernatant
T5931	8422-8425	VB	denotes	was
T5932	8426-8434	VB	denotes	measured
T5933	8435-8449	RB	denotes	quantitatively
T5934	8450-8452	IN	denotes	by
T5935	8453-8458	NN	denotes	ELISA
T5936	8460-8463	DT	denotes	The
T5937	8464-8471	NN	denotes	results
T5938	8472-8475	VB	denotes	are
T5939	8476-8485	VB	denotes	expressed
T5940	8486-8488	IN	denotes	in
T5941	8489-8494	NN	denotes	pg/mL
T6140	8497-8508	JJ	denotes	Statistical
T6141	8509-8517	NN	denotes	Analysis
T6142	8518-8521	DT	denotes	The
T6143	8522-8534	JJ	denotes	experimental
T6144	8535-8542	NN	denotes	results
T6145	8543-8551	VB	denotes	obtained
T6146	8552-8554	IN	denotes	in
T6147	8555-8559	DT	denotes	this
T6148	8560-8565	NN	denotes	study
T6149	8566-8570	VB	denotes	were
T6150	8571-8578	VB	denotes	derived
T6151	8579-8583	IN	denotes	from
T6152	8584-8588	CD	denotes	four
T6153	8589-8597	JJ	denotes	separate
T6154	8598-8609	NN	denotes	experiments
T6155	8610-8614	IN	denotes	with
T6156	8615-8622	JJ	denotes	healthy
T6157	8623-8632	NN	denotes	volunteer
T6158	8633-8639	NN	denotes	donors
T6159	8641-8645	DT	denotes	Each
T6160	8646-8651	NN	denotes	assay
T6161	8652-8655	VB	denotes	was
T6162	8656-8665	VB	denotes	performed
T6163	8666-8668	IN	denotes	in
T6164	8669-8678	NN	denotes	duplicate
T6165	8679-8681	CC	denotes	or
T6166	8682-8692	VB	denotes	triplicate
T6167	8693-8698	WRB	denotes	where
T6168	8699-8710	JJ	denotes	appropriate
T6169	8712-8716	NN	denotes	Data
T6170	8717-8719	VB	denotes	is
T6171	8720-8729	VB	denotes	presented
T6172	8730-8732	IN	denotes	as
T6173	8733-8736	DT	denotes	the
T6174	8737-8741	JJ	denotes	mean
T6175	8742-8743	NN	denotes	±
T6176	8744-8747	NN	denotes	SEM
T6177	8749-8760	JJ	denotes	Statistical
T6178	8761-8772	NN	denotes	differences
T6179	8773-8780	IN	denotes	between
T6180	8781-8787	NN	denotes	groups
T6181	8788-8792	VB	denotes	were
T6182	8793-8803	VB	denotes	determined
T6183	8804-8806	IN	denotes	by
T6184	8807-8814	JJ	denotes	one-way
T6185	8815-8823	NN	denotes	analysis
T6186	8824-8826	IN	denotes	of
T6187	8827-8835	NN	denotes	variance
T6188	8836-8837	-LRB-	denotes	(
T6189	8837-8842	NN	denotes	ANOVA
T6190	8842-8843	-RRB-	denotes	)
T6191	8844-8848	IN	denotes	with
T6192	8849-8850	DT	denotes	a
T6193	8851-8861	NNP	denotes	Bonferroni
T6194	8862-8872	NN	denotes	correction
T6195	8874-8885	JJ	denotes	Statistical
T6196	8886-8898	NN	denotes	significance
T6197	8899-8902	VB	denotes	was
T6198	8903-8910	VB	denotes	defined
T6199	8911-8913	IN	denotes	as
T6200	8914-8915	NN	denotes	P
T6201	8916-8917	SYM	denotes	<
T6202	8918-8922	CD	denotes	0.05
T4026	3598-3600	IN	denotes	on
T4048	3747-3749	TO	denotes	to
R2222	T2794	T2810	arg1Of	approved,in
R2223	T2800	T2796	arg1Of	Program,the
R2224	T2800	T2797	arg1Of	Program,Human
R2225	T2800	T2798	arg1Of	Program,Research
R2226	T2800	T2799	arg1Of	Program,Protections
R2227	T2800	T2801	arg1Of	Program,and
R2228	T2801	T2794	arg1Of	and,approved
R2229	T2801	T2795	arg2Of	and,by
R2230	T2806	T2803	arg1Of	Written,Institutional
R2231	T2806	T2804	arg1Of	Written,Review
R2232	T2806	T2805	arg1Of	Written,Board.
R2233	T2807	T2801	arg2Of	consent,and
R2234	T2807	T2802	arg1Of	consent,the
R2235	T2807	T2806	arg1Of	consent,Written
R2236	T2807	T2808	modOf	consent,to
R2237	T2809	T2808	arg1Of	participate,to
R2238	T2812	T2811	arg1Of	study,the
R2239	T2812	T2813	arg1Of	study,was
R2240	T2812	T2814	arg2Of	study,obtained
R2241	T2814	T2813	arg2Of	obtained,was
R2242	T2814	T2815	arg1Of	obtained,from
R2243	T2814	T2818	arg1Of	obtained,prior
R2244	T2817	T2815	arg2Of	volunteers,from
R2245	T2817	T2816	arg1Of	volunteers,all
R2246	T2818	T2819	arg1Of	prior,to
R2247	T2820	T2819	arg2Of	blood,to
R2248	T2822	T2821	arg1Of	Vacutainers,"donation. Heparinized"
R2249	T2822	T2823	arg1Of	Vacutainers,were
R2250	T2822	T2824	arg2Of	Vacutainers,purchased
R2251	T2824	T2810	arg2Of	purchased,in
R2252	T2824	T2813	modOf	purchased,was
R2253	T2824	T2823	arg2Of	purchased,were
R2254	T2824	T2825	arg1Of	purchased,from
R2255	T2827	T2825	arg2Of	Dickinson,from
R2256	T2827	T2826	arg1Of	Dickinson,Becton
R2257	T2827	T2828	arg1Of	Dickinson,(
R2258	T2830	T2828	arg2Of	Jose,(
R2259	T2830	T2829	arg1Of	Jose,San
R2260	T2830	T2831	arg1Of	Jose,","
R2261	T2832	T2831	arg2Of	CA,","
R2262	T2833	T2828	arg3Of	),(
R2263	T2837	T2834	arg1Of	tubes,1.5-mL
R2264	T2837	T2835	arg1Of	tubes,Eppendorf
R2265	T2837	T2836	arg1Of	tubes,centrifuge
R2266	T2837	T2838	arg1Of	tubes,were
R2267	T2837	T2839	arg2Of	tubes,purchased
R2268	T2839	T2838	arg2Of	purchased,were
R2269	T2839	T2840	arg1Of	purchased,from
R2270	T2842	T2840	arg2Of	Scientific,from
R2271	T2842	T2841	arg1Of	Scientific,Fisher
R2272	T2842	T2843	arg1Of	Scientific,(
R2273	T2844	T2843	arg2Of	Pittsburg,(
R2274	T2844	T2845	arg1Of	Pittsburg,","
R2275	T2844	T2846	arg1Of	Pittsburg,PA
R2276	T2847	T2843	arg3Of	),(
R2277	T2851	T2848	arg1Of	solution,Hank’s
R2278	T2851	T2849	arg1Of	solution,balanced
R2279	T2851	T2850	arg1Of	solution,salt
R2280	T2851	T2852	arg1Of	solution,(
R2281	T2851	T2855	arg1Of	solution,and
R2282	T2853	T2852	arg2Of	HBSS,(
R2283	T2854	T2852	arg3Of	),(
R2284	T2855	T2858	arg1Of	and,were
R2285	T2855	T2859	arg2Of	and,obtained
R2286	T2856	T2855	arg2Of	RPMI,and
R2287	T2856	T2857	arg1Of	RPMI,1640
R2288	T2859	T2858	arg2Of	obtained,were
R2289	T2859	T2860	arg1Of	obtained,from
R2290	T2862	T2860	arg2Of	Scientific,from
R2291	T2862	T2861	arg1Of	Scientific,Irvine
R2292	T2862	T2863	arg1Of	Scientific,(
R2293	T2865	T2863	arg2Of	Ana,(
R2294	T2865	T2864	arg1Of	Ana,Santa
R2295	T2865	T2866	arg1Of	Ana,","
R2296	T2867	T2866	arg2Of	CA,","
R2297	T2868	T2863	arg3Of	),(
R2298	T2871	T2869	arg1Of	coli serotype,LPS
R2299	T2871	T2870	arg1Of	coli serotype,from Escherichia
R2300	T2871	T2872	arg1Of	coli serotype,0111
R2301	T2871	T2873	arg1Of	coli serotype,:
R2302	T2871	T2877	arg1Of	coli serotype,were
R2303	T2871	T2878	arg2Of	coli serotype,purchased
R2304	T2874	T2875	arg1Of	B4,and
R2305	T2875	T2873	arg2Of	and,:
R2306	T2876	T2875	arg2Of	PTX,and
R2307	T2878	T2877	arg2Of	purchased,were
R2308	T2878	T2879	arg1Of	purchased,from
R2309	T2880	T2879	arg2Of	Sigma,from
R2310	T2880	T2881	arg1Of	Sigma,(
R2311	T2883	T2881	arg2Of	Louis,(
R2312	T2883	T2882	arg1Of	Louis,St.
R2313	T2883	T2884	arg1Of	Louis,","
R2314	T2885	T2884	arg2Of	MO,","
R2315	T2886	T2881	arg3Of	),(
R2316	T2889	T2887	arg1Of	inhibitor,The
R2317	T2889	T2888	arg1Of	inhibitor,PKA
R2318	T2889	T2890	arg1Of	inhibitor,","
R2319	T2891	T2890	arg2Of	N-,","
R2320	T2891	T2892	arg1Of	N-,[
R2321	T2893	T2892	arg2Of	2-,[
R2322	T2893	T2894	arg1Of	2-,(
R2323	T2893	T2900	arg1Of	2-,ethyl
R2324	T2896	T2895	arg2Of	p-bromocinnamyl,(
R2325	T2897	T2895	arg3Of	),(
R2326	T2898	T2894	arg2Of	amino,(
R2327	T2898	T2895	arg1Of	amino,(
R2328	T2899	T2894	arg3Of	),(
R2329	T2901	T2892	arg3Of	],[
R2330	T2902	T2889	arg1Of	-5-isoguinolinesulfonamide-2HCl,inhibitor
R2331	T2902	T2903	arg1Of	-5-isoguinolinesulfonamide-2HCl,]
R2332	T2902	T2904	arg1Of	-5-isoguinolinesulfonamide-2HCl,(
R2333	T2902	T2908	arg1Of	-5-isoguinolinesulfonamide-2HCl,was
R2334	T2902	T2909	arg2Of	-5-isoguinolinesulfonamide-2HCl,purchased
R2335	T2905	T2904	arg2Of	H89,(
R2336	T2906	T2904	arg3Of	),(
R2337	T2909	T2907	arg1Of	purchased,","
R2338	T2909	T2908	arg2Of	purchased,was
R2339	T2909	T2910	arg1Of	purchased,from
R2340	T2911	T2910	arg2Of	Calbiochem,from
R2341	T2911	T2912	arg1Of	Calbiochem,(
R2342	T2914	T2912	arg2Of	Jolla,(
R2343	T2914	T2913	arg1Of	Jolla,La
R2344	T2914	T2915	arg1Of	Jolla,","
R2345	T2916	T2915	arg2Of	CA,","
R2346	T2917	T2912	arg3Of	),(
R2347	T2918	T2919	arg1Of	Antibodies,for
R2348	T2918	T2945	arg1Of	Antibodies,and
R2349	T2921	T2920	arg2Of	CREB,phosphorylated
R2350	T2921	T2922	arg1Of	CREB,(
R2351	T2921	T2926	arg1Of	CREB,","
R2352	T2923	T2922	arg2Of	serine,(
R2353	T2923	T2924	arg1Of	serine,133
R2354	T2925	T2922	arg3Of	),(
R2355	T2926	T2935	arg1Of	",",","
R2356	T2928	T2926	arg2Of	I-κBα,","
R2357	T2928	T2927	arg2Of	I-κBα,phosphorylated
R2358	T2928	T2929	arg1Of	I-κBα,(
R2359	T2931	T2932	arg1Of	32,and
R2360	T2932	T2929	arg2Of	and,(
R2361	T2932	T2930	arg1Of	and,serines
R2362	T2933	T2932	arg2Of	36,and
R2363	T2934	T2929	arg3Of	),(
R2364	T2935	T2919	arg2Of	",",for
R2365	T2939	T2935	arg2Of	subunit,","
R2366	T2939	T2936	arg2Of	subunit,phosphorylated
R2367	T2939	T2937	arg1Of	subunit,NF-κB
R2368	T2939	T2938	arg1Of	subunit,p65
R2369	T2939	T2940	arg1Of	subunit,(
R2370	T2941	T2940	arg2Of	serine,(
R2371	T2941	T2942	arg1Of	serine,276
R2372	T2943	T2940	arg3Of	),(
R2373	T2945	T2944	arg1Of	and,","
R2374	T2945	T2948	arg1Of	and,were
R2375	T2945	T2949	arg2Of	and,purchased
R2376	T2947	T2945	arg2Of	antibodies,and
R2377	T2947	T2946	arg1Of	antibodies,secondary
R2378	T2949	T2948	arg2Of	purchased,were
R2379	T2949	T2950	arg1Of	purchased,from
R2380	T2949	T2953	arg1Of	purchased,(
R2381	T2952	T2950	arg2Of	Signaling,from
R2382	T2952	T2951	arg1Of	Signaling,Cell
R2383	T2954	T2953	arg2Of	Beverly,(
R2384	T2954	T2955	arg1Of	Beverly,","
R2385	T2954	T2956	arg1Of	Beverly,MA
R2386	T2957	T2953	arg3Of	),(
R2387	T2959	T2960	arg1Of	T500,and
R2388	T2960	T2958	arg1Of	and,". Dextran"
R2389	T2960	T2962	arg1Of	and,were
R2390	T2960	T2963	arg2Of	and,received
R2391	T2961	T2960	arg2Of	Percoll,and
R2392	T2963	T2948	modOf	received,were
R2393	T2963	T2962	arg2Of	received,were
R2394	T2963	T2964	arg1Of	received,from
R2395	T2967	T2964	arg2Of	Biosciences,from
R2396	T2967	T2965	arg1Of	Biosciences,GE
R2397	T2967	T2966	arg1Of	Biosciences,Healthcare/Amersham
R2398	T2967	T2968	arg1Of	Biosciences,(
R2399	T2969	T2968	arg2Of	Piscataway,(
R2400	T2969	T2970	arg1Of	Piscataway,","
R2401	T2969	T2971	arg1Of	Piscataway,NJ
R2402	T2972	T2968	arg3Of	),(
R2403	T2976	T2973	arg1Of	assay,The
R2404	T2976	T2974	arg1Of	assay,enzyme-linked
R2405	T2976	T2975	arg1Of	assay,immunosorbent
R2406	T2976	T2977	arg1Of	assay,(
R2407	T2976	T2980	arg1Of	assay,for
R2408	T2976	T2982	arg1Of	assay,was
R2409	T2976	T2983	arg2Of	assay,obtained
R2410	T2978	T2977	arg2Of	ELISA,(
R2411	T2979	T2977	arg3Of	),(
R2412	T2981	T2980	arg2Of	TNF-α,for
R2413	T2983	T2982	arg2Of	obtained,was
R2414	T2983	T2984	arg1Of	obtained,from
R2415	T2983	T3004	arg1Of	obtained,and
R2416	T2989	T2985	arg1Of	Systems,Quantikine
R2417	T2989	T2986	arg1Of	Systems,R
R2418	T2989	T2987	arg1Of	Systems,&
R2419	T2989	T2988	arg1Of	Systems,D
R2420	T2989	T2990	arg1Of	Systems,(
R2421	T2991	T2990	arg2Of	Minneapolis,(
R2422	T2991	T2992	arg1Of	Minneapolis,","
R2423	T2991	T2993	arg1Of	Minneapolis,MN
R2424	T2994	T2990	arg3Of	),(
R2425	T2995	T2989	arg1Of	". Tris-glycine",Systems
R2426	T2995	T2996	arg1Of	". Tris-glycine",and
R2427	T2996	T3000	arg1Of	and,","
R2428	T2999	T2996	arg2Of	gels,and
R2429	T2999	T2997	arg1Of	gels,DNA
R2430	T2999	T2998	arg1Of	gels,retardation
R2431	T3000	T2984	arg2Of	",",from
R2432	T3002	T3000	arg2Of	membranes,","
R2433	T3002	T3001	arg1Of	membranes,nitrocellulose
R2434	T3004	T3003	arg1Of	and,","
R2435	T3008	T3005	arg1Of	buffers,western
R2436	T3008	T3006	arg1Of	buffers,blot
R2437	T3008	T3007	arg1Of	buffers,running
R2438	T3008	T3009	arg1Of	buffers,were
R2439	T3008	T3010	arg2Of	buffers,obtained
R2440	T3010	T3004	arg2Of	obtained,and
R2441	T3010	T3009	arg2Of	obtained,were
R2442	T3010	T3011	arg1Of	obtained,from
R2443	T3010	T3013	arg1Of	obtained,(
R2444	T3012	T3011	arg2Of	Invitrogen,from
R2445	T3014	T3013	arg2Of	Carlsbad,(
R2446	T3014	T3015	arg1Of	Carlsbad,","
R2447	T3014	T3016	arg1Of	Carlsbad,CA
R2448	T3017	T3013	arg3Of	),(
R2449	T3018	T3019	arg1Of	NE-PER® nuclear,and
R2450	T3020	T3019	arg2Of	cytoplasmic,and
R2451	T3022	T3018	arg1Of	reagents,NE-PER® nuclear
R2452	T3022	T3020	arg1Of	reagents,cytoplasmic
R2453	T3022	T3021	arg1Of	reagents,extraction
R2454	T3022	T3023	arg1Of	reagents,","
R2455	T3023	T3029	arg1Of	",",","
R2456	T3023	T3037	arg1Of	",",and
R2457	T3028	T3023	arg2Of	kit,","
R2458	T3028	T3024	arg1Of	kit,the
R2459	T3028	T3025	arg1Of	kit,BCA
R2460	T3028	T3026	arg1Of	kit,protein
R2461	T3028	T3027	arg1Of	kit,reagent
R2462	T3035	T3029	arg2Of	Kit,","
R2463	T3035	T3030	arg1Of	Kit,the
R2464	T3035	T3031	arg1Of	Kit,Supersignal
R2465	T3035	T3032	arg1Of	Kit,West
R2466	T3035	T3033	arg1Of	Kit,Pico
R2467	T3035	T3034	arg1Of	Kit,Chemiluminescent
R2468	T3037	T3036	arg1Of	and,","
R2469	T3037	T3043	arg1Of	and,were
R2470	T3037	T3044	arg2Of	and,purchased
R2471	T3041	T3039	arg1Of	EMSA,LightShift
R2472	T3041	T3040	arg1Of	EMSA,Chemiluminescent
R2473	T3042	T3037	arg2Of	Kit,and
R2474	T3042	T3038	arg1Of	Kit,the
R2475	T3042	T3041	arg1Of	Kit,EMSA
R2476	T3044	T3043	arg2Of	purchased,were
R2477	T3044	T3045	arg1Of	purchased,from
R2478	T3046	T3045	arg2Of	Pierce,from
R2479	T3046	T3047	arg1Of	Pierce,(
R2480	T3048	T3047	arg2Of	Rockland,(
R2481	T3048	T3049	arg1Of	Rockland,","
R2482	T3050	T3049	arg2Of	IL,","
R2483	T3051	T3047	arg3Of	),(
R2484	T3052	T3053	arg1Of	CREB,and
R2485	T3054	T3053	arg2Of	NF-κB,and
R2486	T3057	T3052	arg1Of	probes,CREB
R2487	T3057	T3054	arg1Of	probes,NF-κB
R2488	T3057	T3055	arg1Of	probes,DNA
R2489	T3057	T3056	arg1Of	probes,oligonucleotide
R2490	T3057	T3058	arg1Of	probes,were
R2491	T3057	T3059	arg2Of	probes,synthesized
R2492	T3059	T3058	arg2Of	synthesized,were
R2493	T3059	T3062	arg1Of	synthesized,(
R2494	T3061	T3059	arg1Of	IDT,synthesized
R2495	T3061	T3060	arg2Of	IDT,by
R2496	T3063	T3062	arg2Of	Coralville,(
R2497	T3063	T3064	arg1Of	Coralville,","
R2498	T3063	T3065	arg1Of	Coralville,IA
R2499	T3066	T3062	arg3Of	),(
R2500	T3068	T3067	arg1Of	membranes,Nylon
R2501	T3068	T3069	arg1Of	membranes,were
R2502	T3068	T3070	arg2Of	membranes,purchased
R2503	T3070	T3069	arg2Of	purchased,were
R2504	T3070	T3071	arg1Of	purchased,from
R2505	T3074	T3071	arg2Of	Sciences,from
R2506	T3074	T3072	arg1Of	Sciences,Roche
R2507	T3074	T3073	arg1Of	Sciences,Applied
R2508	T3074	T3075	arg1Of	Sciences,(
R2509	T3076	T3075	arg2Of	Indianapolis,(
R2510	T3076	T3077	arg1Of	Indianapolis,","
R2511	T3076	T3078	arg1Of	Indianapolis,IN
R2512	T3079	T3075	arg3Of	),(
R2513	T3083	T3080	arg1Of	quantification,Western
R2514	T3083	T3081	arg1Of	quantification,blot
R2515	T3083	T3082	arg1Of	quantification,band
R2516	T3083	T3084	arg1Of	quantification,was
R2517	T3083	T3085	arg2Of	quantification,performed
R2518	T3085	T3084	arg2Of	performed,was
R2519	T3085	T3086	arg1Of	performed,with
R2520	T3090	T3088	arg1Of	Digitizing,UN-SCAN-IT
R2521	T3090	T3089	arg1Of	Digitizing,Gel
R2522	T3091	T3086	arg2Of	software,with
R2523	T3091	T3087	arg1Of	software,the
R2524	T3091	T3090	arg1Of	software,Digitizing
R2525	T3091	T3092	arg1Of	software,(
R2526	T3094	T3093	arg1Of	Scientific,Silk
R2527	T3094	T3095	arg1Of	Scientific,","
R2528	T3095	T3092	arg2Of	",",(
R2529	T3095	T3097	arg1Of	",",","
R2530	T3096	T3095	arg2Of	Orem,","
R2531	T3098	T3097	arg2Of	UT,","
R2532	T3099	T3092	arg3Of	),(
R2910	T3581	T3578	arg1Of	Cell,Peripheral
R2911	T3581	T3579	arg1Of	Cell,Blood
R2912	T3581	T3580	arg1Of	Cell,Mononuclear
R2913	T3582	T3583	arg1Of	Isolation,and
R2914	T3583	T3577	arg1Of	and,Human
R2915	T3583	T3581	arg1Of	and,Cell
R2916	T3584	T3583	arg2Of	Stimulation,and
R2917	T3587	T3585	arg1Of	cells,Human
R2918	T3587	T3586	arg1Of	cells,mononuclear
R2919	T3587	T3588	arg1Of	cells,were
R2920	T3587	T3589	arg2Of	cells,isolated
R2921	T3589	T3588	arg2Of	isolated,were
R2922	T3589	T3590	arg1Of	isolated,from
R2923	T3593	T3590	arg2Of	blood,from
R2924	T3593	T3591	arg1Of	blood,the
R2925	T3593	T3592	arg1Of	blood,peripheral
R2926	T3593	T3594	arg1Of	blood,of
R2927	T3598	T3594	arg2Of	volunteers,of
R2928	T3598	T3595	arg1Of	volunteers,four
R2929	T3598	T3596	arg1Of	volunteers,healthy
R2930	T3598	T3597	arg1Of	volunteers,human
R2931	T3601	T3599	arg1Of	size,The
R2932	T3601	T3600	arg1Of	size,sample
R2933	T3601	T3602	arg1Of	size,was
R2934	T3601	T3603	arg2Of	size,chosen
R2935	T3603	T3602	arg2Of	chosen,was
R2936	T3603	T3604	arg1Of	chosen,from
R2937	T3603	T3616	arg1Of	chosen,and
R2938	T3607	T3606	arg1Of	published,previously
R2939	T3608	T3604	arg2Of	work,from
R2940	T3608	T3605	arg1Of	work,our
R2941	T3608	T3607	arg1Of	work,published
R2942	T3608	T3609	arg1Of	work,","
R2943	T3608	T3610	arg1Of	work,which
R2944	T3608	T3611	arg1Of	work,demonstrated
R2945	T3615	T3611	arg2Of	isolation,demonstrated
R2946	T3615	T3612	arg1Of	isolation,reliable
R2947	T3615	T3613	arg1Of	isolation,results.10
R2948	T3615	T3614	arg1Of	isolation,Cell
R2949	T3619	T3617	arg1Of	experiments,all
R2950	T3619	T3618	arg1Of	experiments,subsequent
R2951	T3619	T3620	arg1Of	experiments,were
R2952	T3619	T3621	arg2Of	experiments,conducted
R2953	T3621	T3616	arg2Of	conducted,and
R2954	T3621	T3620	arg2Of	conducted,were
R2955	T3621	T3622	arg1Of	conducted,under
R2956	T3623	T3624	arg1Of	sterile,and
R2957	T3625	T3624	arg2Of	pyrogen-free,and
R2958	T3626	T3622	arg2Of	conditions,under
R2959	T3626	T3623	arg1Of	conditions,sterile
R2960	T3626	T3625	arg1Of	conditions,pyrogen-free
R2961	T3627	T3628	arg2Of	Blood,collected
R2962	T3627	T3632	arg1Of	Blood,was
R2963	T3627	T3633	arg2Of	Blood,incubated
R2964	T3628	T3629	arg1Of	collected,in
R2965	T3631	T3629	arg2Of	tubes,in
R2966	T3631	T3630	arg1Of	tubes,heparin
R2967	T3633	T3632	arg2Of	incubated,was
R2968	T3633	T3634	arg1Of	incubated,with
R2969	T3633	T3637	arg1Of	incubated,and
R2970	T3636	T3634	arg2Of	T500,with
R2971	T3636	T3635	arg1Of	T500,Dextran
R2972	T3640	T3638	arg1Of	cells,the
R2973	T3640	T3639	arg1Of	cells,red
R2974	T3640	T3641	arg1Of	cells,were
R2975	T3640	T3642	arg2Of	cells,sedimented
R2976	T3642	T3637	arg2Of	sedimented,and
R2977	T3642	T3641	arg2Of	sedimented,were
R2978	T3642	T3643	arg1Of	sedimented,for
R2979	T3645	T3643	arg2Of	minutes,for
R2980	T3645	T3644	arg1Of	minutes,40
R2981	T3645	T3646	arg1Of	minutes,at
R2982	T3648	T3646	arg2Of	temperature,at
R2983	T3648	T3647	arg1Of	temperature,room
R2984	T3651	T3649	arg1Of	serum,The
R2985	T3651	T3650	arg1Of	serum,resultant
R2986	T3651	T3652	arg1Of	serum,was
R2987	T3651	T3654	arg2Of	serum,washed
R2988	T3651	T3658	arg2Of	serum,separated
R2989	T3654	T3655	arg1Of	washed,with
R2990	T3654	T3657	arg1Of	washed,and
R2991	T3656	T3655	arg2Of	HBSS,with
R2992	T3657	T3652	arg2Of	and,was
R2993	T3657	T3653	arg1Of	and,then
R2994	T3658	T3657	arg2Of	separated,and
R2995	T3658	T3663	arg1Of	separated,according
R2996	T3662	T3658	arg1Of	centrifugation,separated
R2997	T3662	T3659	arg2Of	centrifugation,by
R2998	T3662	T3660	arg1Of	centrifugation,Percoll
R2999	T3662	T3661	arg1Of	centrifugation,gradient
R3000	T3664	T3663	arg2Of	to,according
R3001	T3667	T3664	arg2Of	instructions,to
R3002	T3667	T3665	arg1Of	instructions,the
R3003	T3667	T3666	arg1Of	instructions,manufacturer’s
R3004	T3669	T3668	arg1Of	viability,Cell
R3005	T3669	T3670	arg1Of	viability,was
R3006	T3669	T3671	arg2Of	viability,assessed
R3007	T3671	T3670	arg2Of	assessed,was
R3008	T3671	T3677	arg1Of	assessed,","
R3009	T3671	T3678	arg1Of	assessed,with
R3010	T3676	T3671	arg1Of	exclusion,assessed
R3011	T3676	T3672	arg2Of	exclusion,by
R3012	T3676	T3673	arg1Of	exclusion,trypan
R3013	T3676	T3674	arg1Of	exclusion,blue
R3014	T3676	T3675	arg1Of	exclusion,dye
R3015	T3679	T3678	arg2Of	purity,with
R3016	T3679	T3680	arg1Of	purity,of
R3017	T3681	T3682	arg1Of	greater,than
R3018	T3683	T3682	arg2Of	95,than
R3019	T3684	T3680	arg2Of	%,of
R3020	T3684	T3681	arg1Of	%,greater
R3021	T3686	T3685	arg2Of	cells,Isolated
R3022	T3686	T3687	arg1Of	cells,were
R3023	T3686	T3688	arg2Of	cells,resuspended
R3024	T3688	T3687	arg2Of	resuspended,were
R3025	T3688	T3689	arg1Of	resuspended,in
R3026	T3688	T3701	arg1Of	resuspended,at
R3027	T3690	T3689	arg2Of	RPMI,in
R3028	T3690	T3691	arg1Of	RPMI,1640
R3029	T3690	T3692	arg2Of	RPMI,supplemented
R3030	T3692	T3693	arg1Of	supplemented,with
R3031	T3696	T3694	arg1Of	FBS,10
R3032	T3696	T3695	arg1Of	FBS,%
R3033	T3696	T3697	arg1Of	FBS,and
R3034	T3697	T3693	arg2Of	and,with
R3035	T3698	T3699	arg1Of	5,mM
R3036	T3700	T3697	arg2Of	HEPES,and
R3037	T3700	T3698	arg1Of	HEPES,5
R3038	T3703	T3701	arg2Of	concentration,at
R3039	T3703	T3702	arg1Of	concentration,a
R3040	T3703	T3704	arg1Of	concentration,of
R3041	T3707	T3704	arg2Of	107cells/mL,of
R3042	T3707	T3705	arg1Of	107cells/mL,1
R3043	T3707	T3706	arg1Of	107cells/mL,x
R3044	T3710	T3708	arg1Of	experiment,Each
R3045	T3710	T3709	arg1Of	experiment,subsequent
R3046	T3710	T3711	arg2Of	experiment,listed
R3047	T3710	T3713	arg1Of	experiment,was
R3048	T3710	T3714	arg2Of	experiment,conducted
R3049	T3711	T3712	arg1Of	listed,below
R3050	T3714	T3713	arg2Of	conducted,was
R3051	T3714	T3715	arg1Of	conducted,on
R3052	T3716	T3715	arg2Of	samples,on
R3053	T3716	T3717	arg1Of	samples,of
R3054	T3721	T3717	arg2Of	cells,of
R3055	T3721	T3718	arg1Of	cells,5
R3056	T3721	T3719	arg1Of	cells,x
R3057	T3721	T3720	arg1Of	cells,106
R3058	T3721	T3722	arg1Of	cells,per
R3059	T3724	T3722	arg2Of	group,per
R3060	T3724	T3723	arg1Of	group,treatment
R3061	T3726	T3725	arg2Of	cells,Isolated
R3062	T3726	T3727	arg1Of	cells,were
R3063	T3726	T3728	arg2Of	cells,stimulated
R3064	T3728	T3727	arg2Of	stimulated,were
R3065	T3728	T3729	arg1Of	stimulated,with
R3066	T3728	T3732	arg1Of	stimulated,as
R3067	T3731	T3729	arg2Of	HBSS,with
R3068	T3731	T3730	arg1Of	HBSS,either
R3069	T3735	T3732	arg2Of	control,as
R3070	T3735	T3733	arg1Of	control,a
R3071	T3735	T3734	arg1Of	control,negative
R3072	T3735	T3736	arg1Of	control,","
R3073	T3737	T3738	arg1Of	LPS,(
R3074	T3737	T3742	arg1Of	LPS,","
R3075	T3740	T3738	arg2Of	μg/mL,(
R3076	T3740	T3739	arg1Of	μg/mL,1
R3077	T3741	T3738	arg3Of	),(
R3078	T3742	T3749	arg1Of	",",or
R3079	T3743	T3742	arg2Of	PTX,","
R3080	T3743	T3744	arg1Of	PTX,(
R3081	T3746	T3744	arg2Of	mM,(
R3082	T3746	T3745	arg1Of	mM,20
R3083	T3747	T3744	arg3Of	),(
R3084	T3749	T3736	arg2Of	or,","
R3085	T3749	T3748	arg1Of	or,","
R3086	T3749	T3754	arg1Of	or,","
R3087	T3749	T3755	arg1Of	or,at
R3088	T3749	T3759	arg1Of	or,","
R3089	T3749	T3760	arg1Of	or,for
R3090	T3753	T3749	arg2Of	PTX,or
R3091	T3753	T3750	arg1Of	PTX,concomitant
R3092	T3753	T3751	arg1Of	PTX,LPS
R3093	T3753	T3752	arg1Of	PTX,/
R3094	T3758	T3755	arg2Of	concentrations,at
R3095	T3758	T3756	arg1Of	concentrations,the
R3096	T3758	T3757	arg1Of	concentrations,above-mentioned
R3097	T3762	T3760	arg2Of	minutes,for
R3098	T3762	T3761	arg1Of	minutes,30
R3099	T3762	T3763	arg1Of	minutes,at
R3100	T3764	T3763	arg2Of	37ºC,at
R3101	T3765	T3766	arg1Of	Cells,were
R3102	T3765	T3767	arg2Of	Cells,placed
R3103	T3767	T3766	arg2Of	placed,were
R3104	T3767	T3768	arg1Of	placed,on
R3105	T3767	T3777	arg1Of	placed,and
R3106	T3769	T3768	arg2Of	ice,on
R3107	T3769	T3770	arg1Of	ice,for
R3108	T3772	T3770	arg2Of	minutes,for
R3109	T3772	T3771	arg1Of	minutes,10
R3110	T3772	T3774	arg1Of	minutes,stop
R3111	T3774	T3770	arg3Of	stop,for
R3112	T3774	T3773	arg1Of	stop,to
R3113	T3776	T3774	arg2Of	reaction,stop
R3114	T3776	T3775	arg1Of	reaction,the
R3115	T3779	T3778	arg1Of	samples,the
R3116	T3779	T3780	arg1Of	samples,were
R3117	T3779	T3782	arg2Of	samples,stored
R3118	T3782	T3777	arg2Of	stored,and
R3119	T3782	T3780	arg2Of	stored,were
R3120	T3782	T3781	arg1Of	stored,then
R3121	T3782	T3783	arg1Of	stored,at
R3122	T3784	T3783	arg2Of	−70°C,at
R3123	T3784	T3785	arg1Of	−70°C,for
R3124	T3787	T3785	arg2Of	analysis,for
R3125	T3787	T3786	arg1Of	analysis,further
R3126	T3790	T3789	arg1Of	times,stimulation
R3127	T3790	T3791	arg1Of	times,and
R3128	T3791	T3788	arg1Of	and,The
R3129	T3791	T3801	arg1Of	and,were
R3130	T3791	T3802	arg2Of	and,determined
R3131	T3792	T3791	arg2Of	concentrations,and
R3132	T3792	T3793	arg1Of	concentrations,of
R3133	T3794	T3795	arg1Of	LPS,and
R3134	T3795	T3793	arg2Of	and,of
R3135	T3795	T3797	arg2Of	and,utilized
R3136	T3796	T3795	arg2Of	PTX,and
R3137	T3797	T3798	arg1Of	utilized,in
R3138	T3800	T3798	arg2Of	study,in
R3139	T3800	T3799	arg1Of	study,this
R3140	T3802	T3801	arg2Of	determined,were
R3141	T3802	T3803	arg1Of	determined,in
R3142	T3806	T3803	arg2Of	studies,in
R3143	T3806	T3804	arg1Of	studies,previous
R3144	T3806	T3805	arg1Of	studies,pilot
R3145	T3806	T3807	arg2Of	studies,done
R3146	T3807	T3808	arg1Of	done,in
R3147	T3810	T3808	arg2Of	laboratory,in
R3148	T3810	T3809	arg1Of	laboratory,our
R3149	T3810	T3811	arg1Of	laboratory,","
R3150	T3810	T3812	arg1Of	laboratory,which
R3151	T3810	T3813	arg1Of	laboratory,examined
R3152	T3815	T3813	arg2Of	effects,examined
R3153	T3815	T3814	arg1Of	effects,the
R3154	T3815	T3816	arg1Of	effects,of
R3155	T3815	T3821	arg1Of	effects,on
R3156	T3818	T3816	arg2Of	concentrations,of
R3157	T3818	T3817	arg1Of	concentrations,increasing
R3158	T3818	T3819	arg1Of	concentrations,of
R3159	T3820	T3819	arg2Of	PTX,of
R3160	T3824	T3821	arg2Of	production,on
R3161	T3824	T3822	arg1Of	production,LPS-induced
R3162	T3824	T3823	arg1Of	production,TNF-α
R3163	T3825	T3826	arg1Of	LPS,at
R3164	T3825	T3834	arg1Of	LPS,at
R3165	T3825	T3840	arg1Of	LPS,were
R3166	T3828	T3826	arg2Of	concentration,at
R3167	T3828	T3827	arg1Of	concentration,a
R3168	T3828	T3829	arg1Of	concentration,of
R3169	T3831	T3832	arg1Of	μg/mL,and
R3170	T3832	T3829	arg2Of	and,of
R3171	T3832	T3830	arg1Of	and,1
R3172	T3833	T3832	arg2Of	PTX,and
R3173	T3836	T3834	arg2Of	concentration,at
R3174	T3836	T3835	arg1Of	concentration,a
R3175	T3836	T3837	arg1Of	concentration,of
R3176	T3839	T3837	arg2Of	mM,of
R3177	T3839	T3838	arg1Of	mM,20
R3178	T3843	T3840	arg2Of	concentrations,were
R3179	T3843	T3841	arg1Of	concentrations,the
R3180	T3843	T3842	arg1Of	concentrations,minimum
R3181	T3843	T3844	arg1Of	concentrations,necessary
R3182	T3846	T3844	arg2Of	produce,necessary
R3183	T3846	T3845	arg1Of	produce,to
R3184	T3846	T3857	arg1Of	produce,","
R3185	T3846	T3858	arg1Of	produce,respectively
R3186	T3846	T3859	arg1Of	produce,","
R3187	T3846	T3860	arg1Of	produce,in
R3188	T3848	T3849	arg1Of	reproducible,and
R3189	T3850	T3849	arg2Of	reliable,and
R3190	T3851	T3852	arg1Of	up-regulation,and
R3191	T3852	T3846	arg2Of	and,produce
R3192	T3852	T3847	arg1Of	and,a
R3193	T3852	T3848	arg1Of	and,reproducible
R3194	T3852	T3850	arg1Of	and,reliable
R3195	T3852	T3854	arg1Of	and,in
R3196	T3853	T3852	arg2Of	down-regulation,and
R3197	T3856	T3854	arg2Of	production,in
R3198	T3856	T3855	arg1Of	production,TNF-α
R3199	T3862	T3860	arg2Of	assays,in
R3200	T3862	T3861	arg1Of	assays,quantitative
R3201	T3862	T3863	arg1Of	assays,(
R3202	T3864	T3863	arg2Of	data,(
R3203	T3864	T3866	arg2Of	data,shown
R3204	T3866	T3865	arg1Of	shown,not
R3205	T3867	T3863	arg3Of	),(
R3302	T4023	T4024	arg1Of	Effect,of
R3303	T4023	T4026	arg1Of	Effect,on
R3304	T4023	T4030	arg1Of	Effect,by
R3305	T4025	T4024	arg2Of	PTX,of
R3306	T4029	T4026	arg2Of	production,on
R3307	T4029	T4027	arg1Of	production,LPS-induced
R3308	T4029	T4028	arg1Of	production,TNF-α
R3309	T4032	T4030	arg2Of	cells,by
R3310	T4032	T4031	arg1Of	cells,mononuclear
R3311	T4034	T4033	arg1Of	concentration,The
R3323	T4044	T4045	arg1Of	media,of
R3324	T4044	T4063	arg1Of	media,","
R3325	T4044	T4064	arg1Of	media,at
R3326	T4046	T4045	arg2Of	cells,of
R3327	T4046	T4047	arg2Of	cells,exposed
R3328	T4047	T4048	arg1Of	exposed,to
R3329	T4050	T4048	arg2Of	treatments,to
R3330	T4050	T4049	arg1Of	treatments,the
R3331	T4050	T4051	arg2Of	treatments,described
R3332	T4051	T4052	arg1Of	described,above
R3333	T4051	T4053	arg1Of	described,after
R3334	T4051	T4055	arg1Of	described,for
R3335	T4054	T4053	arg2Of	stimulation,after
R3336	T4056	T4055	arg2Of	up,for
R3337	T4056	T4057	arg1Of	up,to
R3338	T4059	T4057	arg2Of	maximum,to
R3339	T4059	T4058	arg1Of	maximum,a
R3340	T4059	T4060	arg1Of	maximum,of
R3341	T4062	T4060	arg2Of	hours,of
R3342	T4062	T4061	arg1Of	hours,18
R3343	T4066	T4064	arg2Of	intervals,at
R3344	T4066	T4065	arg1Of	intervals,3-hour
R3345	T4068	T4067	arg1Of	results,The
R3346	T4068	T4069	arg1Of	results,are
R3347	T4068	T4070	arg2Of	results,expressed
R3348	T4070	T4069	arg2Of	expressed,are
R3349	T4070	T4071	arg1Of	expressed,in
R3350	T4072	T4071	arg2Of	pg/mL,in
R3696	T4475	T4476	arg1Of	SDS-PAGE,and
R3697	T4476	T4478	arg1Of	and,(
R3698	T4477	T4476	arg2Of	Immunoblotting,and
R3699	T4479	T4480	arg1Of	I-κBα,","
R3700	T4480	T4484	arg1Of	",",and
R3701	T4482	T4480	arg2Of	p65,","
R3702	T4482	T4481	arg1Of	p65,NF-κB
R3703	T4484	T4478	arg2Of	and,(
R3704	T4484	T4483	arg1Of	and,","
R3705	T4485	T4484	arg2Of	CREB,and
R3706	T4486	T4478	arg3Of	),(
R3707	T4488	T4487	arg2Of	stimulation,Following
R3708	T4490	T4491	arg1Of	PBMCs,were
R3709	T4490	T4492	arg2Of	PBMCs,washed
R3710	T4490	T4497	arg2Of	PBMCs,centrifuged
R3711	T4490	T4499	arg1Of	PBMCs,collect
R3712	T4492	T4493	arg1Of	washed,with
R3713	T4492	T4496	arg1Of	washed,and
R3714	T4495	T4493	arg2Of	PBS,with
R3715	T4495	T4494	arg1Of	PBS,ice-cold
R3716	T4496	T4487	arg1Of	and,Following
R3717	T4496	T4489	arg1Of	and,","
R3718	T4496	T4491	arg2Of	and,were
R3719	T4497	T4496	arg2Of	centrifuged,and
R3720	T4499	T4497	arg3Of	collect,centrifuged
R3721	T4499	T4498	arg1Of	collect,to
R3722	T4502	T4499	arg2Of	pellet,collect
R3723	T4502	T4500	arg1Of	pellet,a
R3724	T4502	T4501	arg1Of	pellet,cell
R3725	T4504	T4503	arg1Of	pellet,The
R3726	T4504	T4505	arg1Of	pellet,was
R3727	T4504	T4506	arg2Of	pellet,resuspended
R3728	T4506	T4505	arg2Of	resuspended,was
R3729	T4506	T4507	arg1Of	resuspended,in
R3730	T4511	T4507	arg2Of	buffer,in
R3731	T4511	T4508	arg1Of	buffer,ice-cold
R3732	T4511	T4509	arg1Of	buffer,SDS
R3733	T4511	T4510	arg1Of	buffer,sample
R3734	T4511	T4512	arg2Of	buffer,supplemented
R3735	T4512	T4513	arg1Of	supplemented,with
R3736	T4514	T4515	arg1Of	100,mM
R3737	T4516	T4513	arg2Of	dithiothreitol,with
R3738	T4516	T4514	arg1Of	dithiothreitol,100
R3739	T4517	T4518	arg1Of	Cytoplasmic,and
R3740	T4519	T4518	arg2Of	nuclear,and
R3741	T4520	T4517	arg1Of	extracts,Cytoplasmic
R3742	T4520	T4519	arg1Of	extracts,nuclear
R3743	T4520	T4521	arg1Of	extracts,were
R3744	T4520	T4522	arg2Of	extracts,isolated
R3745	T4522	T4521	arg2Of	isolated,were
R3746	T4522	T4523	arg1Of	isolated,with
R3747	T4522	T4536	arg1Of	isolated,according
R3748	T4525	T4526	arg1Of	nuclear,and
R3749	T4527	T4526	arg2Of	cytoplasmic,and
R3750	T4529	T4523	arg2Of	reagents,with
R3751	T4529	T4524	arg1Of	reagents,NE-PER
R3752	T4529	T4525	arg1Of	reagents,nuclear
R3753	T4529	T4527	arg1Of	reagents,cytoplasmic
R3754	T4529	T4528	arg1Of	reagents,extraction
R3755	T4529	T4530	arg1Of	reagents,with
R3756	T4535	T4530	arg2Of	Cocktail,with
R3757	T4535	T4531	arg1Of	Cocktail,1x
R3758	T4535	T4532	arg1Of	Cocktail,Halt
R3759	T4535	T4533	arg1Of	Cocktail,Protease
R3760	T4535	T4534	arg1Of	Cocktail,Inhibitor
R3761	T4537	T4536	arg2Of	to,according
R3762	T4540	T4537	arg2Of	instructions,to
R3763	T4540	T4538	arg1Of	instructions,the
R3764	T4540	T4539	arg1Of	instructions,manufacturer’s
R3765	T4542	T4541	arg1Of	concentrations,Protein
R3766	T4542	T4543	arg1Of	concentrations,were
R3767	T4542	T4544	arg2Of	concentrations,determined
R3768	T4544	T4543	arg2Of	determined,were
R3769	T4544	T4545	arg1Of	determined,with
R3770	T4544	T4554	arg1Of	determined,according
R3771	T4550	T4545	arg2Of	kit,with
R3772	T4550	T4546	arg1Of	kit,the
R3773	T4550	T4547	arg1Of	kit,BCA
R3774	T4550	T4548	arg1Of	kit,protein
R3775	T4550	T4549	arg1Of	kit,reagent
R3776	T4550	T4551	arg1Of	kit,for
R3777	T4553	T4551	arg2Of	sample,for
R3778	T4553	T4552	arg1Of	sample,each
R3779	T4555	T4554	arg2Of	to,according
R3780	T4558	T4555	arg2Of	curve,to
R3781	T4558	T4556	arg1Of	curve,a
R3782	T4558	T4557	arg1Of	curve,standardized
R3783	T4558	T4559	arg1Of	curve,for
R3784	T4560	T4559	arg2Of	albumin,for
R3785	T4562	T4561	arg1Of	lysates,Cell
R3786	T4562	T4563	arg1Of	lysates,were
R3787	T4562	T4564	arg2Of	lysates,collected
R3788	T4564	T4563	arg2Of	collected,were
R3789	T4564	T4565	arg1Of	collected,by
R3790	T4566	T4565	arg2Of	boiling,by
R3791	T4566	T4569	arg1Of	boiling,in
R3792	T4568	T4566	arg2Of	samples,boiling
R3793	T4568	T4567	arg1Of	samples,the
R3794	T4573	T4569	arg2Of	bath,in
R3795	T4573	T4570	arg1Of	bath,a
R3796	T4573	T4571	arg1Of	bath,100ºC
R3797	T4573	T4572	arg1Of	bath,water
R3798	T4573	T4574	arg1Of	bath,for
R3799	T4576	T4574	arg2Of	minutes,for
R3800	T4576	T4575	arg1Of	minutes,5
R3801	T4578	T4577	arg1Of	μg,Ten
R3802	T4578	T4579	arg1Of	μg,of
R3803	T4578	T4581	arg1Of	μg,per
R3804	T4578	T4583	arg1Of	μg,was
R3805	T4578	T4584	arg2Of	μg,separated
R3806	T4578	T4597	arg2Of	μg,transferred
R3807	T4580	T4579	arg2Of	protein,of
R3808	T4582	T4581	arg2Of	sample,per
R3809	T4584	T4589	arg1Of	separated,through
R3810	T4584	T4596	arg1Of	separated,and
R3811	T4588	T4584	arg1Of	electrophoresis,separated
R3812	T4588	T4585	arg2Of	electrophoresis,by
R3813	T4588	T4586	arg1Of	electrophoresis,SDS-polyacrylamide
R3814	T4588	T4587	arg1Of	electrophoresis,gel
R3815	T4590	T4591	arg1Of	8–16,%
R3816	T4595	T4589	arg2Of	gels,through
R3817	T4595	T4590	arg1Of	gels,8–16
R3818	T4595	T4592	arg1Of	gels,tris-glycine
R3819	T4595	T4593	arg1Of	gels,polyacrylamide
R3820	T4595	T4594	arg1Of	gels,gradient
R3821	T4596	T4583	arg2Of	and,was
R3822	T4597	T4596	arg2Of	transferred,and
R3823	T4597	T4598	modOf	transferred,to
R3824	T4599	T4598	arg1Of	nitrocellulose,to
R3825	T4600	T4599	arg2Of	membranes,nitrocellulose
R3826	T4602	T4601	arg1Of	membranes,The
R3827	T4602	T4603	arg1Of	membranes,were
R3828	T4602	T4604	arg2Of	membranes,blocked
R3829	T4604	T4603	arg2Of	blocked,were
R3830	T4604	T4605	arg1Of	blocked,with
R3831	T4606	T4607	arg1Of	5,%
R3832	T4608	T4605	arg2Of	milk,with
R3833	T4608	T4606	arg1Of	milk,5
R3834	T4608	T4609	arg1Of	milk,in
R3835	T4611	T4609	arg2Of	saline/Tween,in
R3836	T4611	T4610	arg1Of	saline/Tween,Tris-buffered
R3837	T4611	T4612	arg1Of	saline/Tween,20
R3838	T4611	T4613	arg1Of	saline/Tween,(
R3839	T4611	T4621	arg1Of	saline/Tween,for
R3840	T4615	T4613	arg2Of	Scientific,(
R3841	T4615	T4614	arg1Of	Scientific,Fischer
R3842	T4615	T4616	arg1Of	Scientific,","
R3843	T4617	T4616	arg2Of	Pittsburgh,","
R3844	T4617	T4618	arg1Of	Pittsburgh,","
R3845	T4619	T4618	arg2Of	PA,","
R3846	T4620	T4613	arg3Of	),(
R3847	T4623	T4621	arg2Of	hour,for
R3848	T4623	T4622	arg1Of	hour,1
R3849	T4625	T4624	arg1Of	extracts,Cytoplasmic
R3850	T4625	T4626	arg1Of	extracts,were
R3851	T4625	T4627	arg2Of	extracts,incubated
R3852	T4627	T4626	arg2Of	incubated,were
R3853	T4627	T4628	arg1Of	incubated,with
R3854	T4627	T4637	arg1Of	incubated,","
R3855	T4627	T4638	arg1Of	incubated,while
R3856	T4631	T4628	arg2Of	antibody,with
R3857	T4631	T4629	arg2Of	antibody,phosphorylated
R3858	T4631	T4630	arg1Of	antibody,I-κBα
R3859	T4631	T4632	arg1Of	antibody,(
R3860	T4633	T4634	arg1Of	1,:
R3861	T4634	T4632	arg2Of	:,(
R3862	T4635	T4634	arg2Of	200,:
R3863	T4636	T4632	arg3Of	),(
R3864	T4640	T4639	arg1Of	extracts,nuclear
R3865	T4640	T4641	arg1Of	extracts,were
R3866	T4640	T4642	arg2Of	extracts,incubated
R3867	T4642	T4638	arg2Of	incubated,while
R3868	T4642	T4641	arg2Of	incubated,were
R3869	T4642	T4643	arg1Of	incubated,with
R3870	T4642	T4664	arg1Of	incubated,at
R3871	T4648	T4644	arg1Of	antibody,either
R3872	T4648	T4645	arg2Of	antibody,phosphorylated
R3873	T4648	T4646	arg1Of	antibody,NF-κB
R3874	T4648	T4647	arg1Of	antibody,p65
R3875	T4648	T4649	arg1Of	antibody,(
R3876	T4648	T4654	arg1Of	antibody,or
R3877	T4650	T4651	arg1Of	1,:
R3878	T4651	T4649	arg2Of	:,(
R3879	T4652	T4651	arg2Of	500,:
R3880	T4653	T4649	arg3Of	),(
R3881	T4654	T4643	arg2Of	or,with
R3882	T4657	T4654	arg2Of	antibody,or
R3883	T4657	T4655	arg2Of	antibody,phosphorylated
R3884	T4657	T4656	arg1Of	antibody,CREB
R3885	T4657	T4658	arg1Of	antibody,(
R3886	T4657	T4663	arg1Of	antibody,overnight
R3887	T4659	T4660	arg1Of	1,:
R3888	T4660	T4658	arg2Of	:,(
R3889	T4661	T4660	arg2Of	500,:
R3890	T4662	T4658	arg3Of	),(
R3891	T4665	T4664	arg2Of	4°C,at
R3892	T4665	T4666	arg1Of	4°C,in
R3893	T4668	T4666	arg2Of	experiments,in
R3894	T4668	T4667	arg1Of	experiments,separate
R3895	T4670	T4669	arg1Of	membranes,The
R3896	T4670	T4671	arg1Of	membranes,were
R3897	T4670	T4672	arg2Of	membranes,washed
R3898	T4670	T4678	arg2Of	membranes,incubated
R3899	T4672	T4673	arg1Of	washed,with
R3900	T4672	T4677	arg1Of	washed,and
R3901	T4675	T4673	arg2Of	saline/Tween,with
R3902	T4675	T4674	arg1Of	saline/Tween,Tris-buffered
R3903	T4675	T4676	arg1Of	saline/Tween,20
R3904	T4677	T4671	arg2Of	and,were
R3905	T4678	T4677	arg2Of	incubated,and
R3906	T4678	T4679	arg1Of	incubated,for
R3907	T4678	T4682	arg1Of	incubated,at
R3908	T4681	T4679	arg2Of	hour,for
R3909	T4681	T4680	arg1Of	hour,1
R3910	T4684	T4682	arg2Of	temperature,at
R3911	T4684	T4683	arg1Of	temperature,room
R3912	T4684	T4685	arg1Of	temperature,with
R3913	T4688	T4685	arg2Of	antibody,with
R3914	T4688	T4686	arg1Of	antibody,the
R3915	T4688	T4687	arg1Of	antibody,secondary
R3916	T4693	T4690	arg1Of	IgG,horseradish
R3917	T4693	T4691	arg1Of	IgG,peroxidase-linked
R3918	T4693	T4692	arg1Of	IgG,anti-rabbit
R3919	T4693	T4694	arg1Of	IgG,diluted
R3920	T4694	T4671	modOf	diluted,were
R3921	T4694	T4689	arg1Of	diluted,","
R3922	T4694	T4696	arg1Of	diluted,:
R3923	T4695	T4694	arg2Of	1,diluted
R3924	T4697	T4694	arg3Of	2000,diluted
R3925	T4697	T4698	arg1Of	2000,in
R3926	T4699	T4698	arg2Of	blocking,in
R3927	T4700	T4699	arg2Of	solution,blocking
R3928	T4703	T4701	arg2Of	washing,After
R3929	T4703	T4702	arg2Of	washing,repeated
R3930	T4703	T4704	arg1Of	washing,of
R3931	T4706	T4704	arg2Of	membrane,of
R3932	T4706	T4705	arg1Of	membrane,the
R3933	T4713	T4708	arg1Of	Kit,the
R3934	T4713	T4709	arg1Of	Kit,Supersignal
R3935	T4713	T4710	arg1Of	Kit,West
R3936	T4713	T4711	arg1Of	Kit,Pico
R3937	T4713	T4712	arg1Of	Kit,Chemiluminescent
R3938	T4713	T4714	arg1Of	Kit,was
R3939	T4713	T4715	arg2Of	Kit,applied
R3940	T4715	T4701	arg1Of	applied,After
R3941	T4715	T4707	arg1Of	applied,","
R3942	T4715	T4714	arg2Of	applied,was
R3943	T4715	T4716	arg1Of	applied,for
R3944	T4718	T4716	arg2Of	detection,for
R3945	T4718	T4717	arg1Of	detection,antibody
R3946	T4718	T4719	arg1Of	detection,per
R3947	T4722	T4719	arg2Of	instructions,per
R3948	T4722	T4720	arg1Of	instructions,the
R3949	T4722	T4721	arg1Of	instructions,manufacturer’s
R3950	T4725	T4723	arg1Of	data,Western
R3951	T4725	T4724	arg1Of	data,blot
R3952	T4725	T4726	arg1Of	data,is
R3953	T4725	T4727	arg2Of	data,presented
R3954	T4727	T4726	arg2Of	presented,is
R3955	T4727	T4728	arg1Of	presented,as
R3956	T4730	T4728	arg2Of	percentage,as
R3957	T4730	T4729	arg1Of	percentage,a
R3958	T4730	T4731	arg1Of	percentage,of
R3959	T4733	T4731	arg2Of	stimulation,of
R3960	T4733	T4732	arg1Of	stimulation,LPS
R3961	T4735	T4734	arg1Of	percentage,The
R3962	T4735	T4736	arg1Of	percentage,of
R3963	T4735	T4739	arg1Of	percentage,was
R3964	T4735	T4740	arg2Of	percentage,calculated
R3965	T4738	T4736	arg2Of	stimulation,of
R3966	T4738	T4737	arg1Of	stimulation,LPS
R3967	T4740	T4739	arg2Of	calculated,was
R3968	T4740	T4741	arg1Of	calculated,by
R3969	T4742	T4741	arg2Of	dividing,by
R3970	T4747	T4742	arg2Of	total,dividing
R3971	T4747	T4743	arg1Of	total,the
R3972	T4747	T4744	arg1Of	total,mean
R3973	T4747	T4745	arg1Of	total,band
R3974	T4747	T4746	arg1Of	total,pixel
R3975	T4747	T4748	arg1Of	total,for
R3976	T4751	T4748	arg2Of	arm,for
R3977	T4751	T4749	arg1Of	arm,each
R3978	T4751	T4750	arg1Of	arm,treatment
R3979	T4751	T4752	arg2Of	arm,divided
R3980	T4757	T4752	arg1Of	total,divided
R3981	T4757	T4753	arg2Of	total,by
R3982	T4757	T4754	arg1Of	total,the
R3983	T4757	T4755	arg1Of	total,mean
R3984	T4757	T4756	arg1Of	total,pixel
R3985	T4757	T4758	arg1Of	total,of
R3986	T4759	T4758	arg2Of	samples,of
R3987	T4759	T4760	arg2Of	samples,stimulated
R3988	T4759	T4764	arg1Of	samples,multiplying
R3989	T4760	T4761	arg1Of	stimulated,with
R3990	T4760	T4763	arg1Of	stimulated,and
R3991	T4762	T4761	arg2Of	LPS,with
R3992	T4764	T4763	arg2Of	multiplying,and
R3993	T4764	T4765	arg1Of	multiplying,by
R3994	T4766	T4765	arg2Of	100,by
R3995	T4770	T4769	arg1Of	stimulation,LPS
R3996	T4770	T4771	arg1Of	stimulation,is
R3997	T4770	T4772	arg2Of	stimulation,reported
R3998	T4772	T4771	arg2Of	reported,is
R3999	T4772	T4773	arg1Of	reported,as
R4000	T4772	T4776	arg1Of	reported,and
R4001	T4775	T4773	arg2Of	%,as
R4002	T4775	T4774	arg1Of	%,100
R4003	T4776	T4767	arg1Of	and,Thus
R4004	T4776	T4768	arg1Of	and,","
R4005	T4777	T4778	arg1Of	each,of
R4006	T4777	T4782	arg1Of	each,is
R4007	T4777	T4783	arg2Of	each,reported
R4008	T4781	T4778	arg2Of	arms,of
R4009	T4781	T4779	arg1Of	arms,the
R4010	T4781	T4780	arg1Of	arms,treatment
R4011	T4783	T4776	arg2Of	reported,and
R4012	T4783	T4782	arg2Of	reported,is
R4013	T4783	T4784	arg1Of	reported,as
R4014	T4786	T4784	arg2Of	percent,as
R4015	T4786	T4785	arg1Of	percent,a
R4016	T4786	T4787	arg1Of	percent,of
R4017	T4789	T4787	arg2Of	stimulation,of
R4018	T4789	T4788	arg1Of	stimulation,LPS
R4358	T5231	T5232	arg1Of	NF-κB,and
R4359	T5233	T5232	arg2Of	CREB,and
R4360	T5237	T5231	arg1Of	Assay,NF-κB
R4361	T5237	T5233	arg1Of	Assay,CREB
R4362	T5237	T5234	arg1Of	Assay,Electrophoretic
R4363	T5237	T5235	arg1Of	Assay,Mobility
R4364	T5237	T5236	arg1Of	Assay,Shift
R4365	T5237	T5238	arg1Of	Assay,(
R4366	T5239	T5238	arg2Of	EMSA,(
R4367	T5240	T5238	arg3Of	),(
R4368	T5245	T5243	arg1Of	EMSA,LightShift
R4369	T5245	T5244	arg1Of	EMSA,Chemiluminescent
R4370	T5246	T5241	arg1Of	Kit,The
R4371	T5246	T5242	arg1Of	Kit,non-radioactive
R4372	T5246	T5245	arg1Of	Kit,EMSA
R4373	T5246	T5247	arg1Of	Kit,was
R4374	T5246	T5248	arg2Of	Kit,used
R4375	T5248	T5247	arg2Of	used,was
R4376	T5248	T5249	modOf	used,to
R4377	T5250	T5249	arg1Of	detect,to
R4378	T5252	T5250	arg2Of	interactions,detect
R4379	T5252	T5251	arg1Of	interactions,DNA-protein
R4380	T5259	T5253	arg1Of	oligonucleotide,The
R4381	T5259	T5254	arg1Of	oligonucleotide,NF-κB
R4382	T5259	T5255	arg1Of	oligonucleotide,3′
R4383	T5259	T5256	arg1Of	oligonucleotide,biotin
R4384	T5259	T5257	arg1Of	oligonucleotide,end-labeled
R4385	T5259	T5258	arg1Of	oligonucleotide,DNA
R4386	T5259	T5260	arg2Of	oligonucleotide,used
R4387	T5259	T5267	arg1Of	oligonucleotide,was
R4388	T5260	T5261	arg1Of	used,as
R4389	T5263	T5261	arg2Of	probe,as
R4390	T5263	T5262	arg1Of	probe,a
R4391	T5263	T5264	arg1Of	probe,for
R4392	T5266	T5264	arg2Of	EMSA,for
R4393	T5266	T5265	arg1Of	EMSA,the
R4394	T5272	T5267	arg2Of	construct,was
R4395	T5272	T5268	arg1Of	construct,a
R4396	T5272	T5269	arg1Of	construct,42-bp
R4397	T5272	T5270	arg1Of	construct,double
R4398	T5272	T5271	arg1Of	construct,stranded
R4399	T5272	T5273	arg1Of	construct,(
R4400	T5272	T5278	arg1Of	construct,containing
R4401	T5276	T5273	arg2Of	3′,(
R4402	T5276	T5274	arg1Of	3′,5′-TTGTTACAA-GGGGACTTTCCGCTGGGGACTTTCCAGGGAGGC
R4403	T5276	T5275	arg1Of	3′,–
R4404	T5277	T5273	arg3Of	),(
R4405	T5284	T5278	arg2Of	sites,containing
R4406	T5284	T5279	arg1Of	sites,two
R4407	T5284	T5280	arg1Of	sites,tandemly
R4408	T5284	T5281	arg2Of	sites,repeated
R4409	T5284	T5282	arg1Of	sites,NF-κB
R4410	T5284	T5283	arg1Of	sites,binding
R4411	T5284	T5285	arg1Of	sites,(
R4412	T5286	T5285	arg2Of	underlined,(
R4413	T5287	T5285	arg3Of	),(
R4414	T5288	T5289	arg1Of	Specificity,was
R4415	T5288	T5290	arg2Of	Specificity,determined
R4416	T5290	T5289	arg2Of	determined,was
R4417	T5294	T5290	arg1Of	assay,determined
R4418	T5294	T5291	arg2Of	assay,by
R4419	T5294	T5292	arg1Of	assay,a
R4420	T5294	T5293	arg1Of	assay,competition
R4421	T5294	T5295	arg1Of	assay,with
R4422	T5297	T5295	arg2Of	addition,with
R4423	T5297	T5296	arg1Of	addition,the
R4424	T5297	T5298	arg1Of	addition,of
R4425	T5300	T5299	arg1Of	molar,200
R4426	T5301	T5298	arg2Of	excess,of
R4427	T5301	T5300	arg1Of	excess,molar
R4428	T5301	T5302	arg1Of	excess,of
R4429	T5305	T5303	arg1Of	stranded,unlabeled
R4430	T5305	T5304	arg1Of	stranded,double
R4431	T5307	T5302	arg2Of	oligonucleotide,of
R4432	T5307	T5305	arg1Of	oligonucleotide,stranded
R4433	T5307	T5306	arg1Of	oligonucleotide,NF-κB
R4434	T5314	T5308	arg1Of	oligonucleotide,The
R4435	T5314	T5309	arg1Of	oligonucleotide,CREB
R4436	T5314	T5310	arg1Of	oligonucleotide,3′
R4437	T5314	T5311	arg1Of	oligonucleotide,biotin
R4438	T5314	T5312	arg1Of	oligonucleotide,end-labeled
R4439	T5314	T5313	arg1Of	oligonucleotide,DNA
R4440	T5314	T5315	arg2Of	oligonucleotide,used
R4441	T5314	T5322	arg1Of	oligonucleotide,was
R4442	T5315	T5316	arg1Of	used,as
R4443	T5318	T5316	arg2Of	probe,as
R4444	T5318	T5317	arg1Of	probe,a
R4445	T5318	T5319	arg1Of	probe,for
R4446	T5321	T5319	arg2Of	EMSA,for
R4447	T5321	T5320	arg1Of	EMSA,the
R4448	T5327	T5322	arg2Of	construct,was
R4449	T5327	T5323	arg1Of	construct,a
R4450	T5327	T5324	arg1Of	construct,23-bp
R4451	T5327	T5325	arg1Of	construct,double
R4452	T5327	T5326	arg1Of	construct,stranded
R4453	T5327	T5328	arg1Of	construct,(
R4454	T5327	T5336	arg1Of	construct,containing
R4455	T5334	T5328	arg2Of	3′,(
R4456	T5334	T5329	arg1Of	3′,5′-TTT
R4457	T5334	T5330	arg1Of	3′,TCG
R4458	T5334	T5331	arg1Of	3′,AGC
R4459	T5334	T5332	arg1Of	3′,TCTGACGTCAGA-GC
R4460	T5334	T5333	arg1Of	3′,–
R4461	T5335	T5328	arg3Of	),(
R4462	T5340	T5336	arg2Of	sequence,containing
R4463	T5340	T5337	arg1Of	sequence,the
R4464	T5340	T5338	arg1Of	sequence,CRE
R4465	T5340	T5339	arg1Of	sequence,consensus
R4466	T5340	T5341	arg1Of	sequence,(
R4467	T5342	T5341	arg2Of	underlined,(
R4468	T5343	T5341	arg3Of	),(
R4469	T5344	T5345	arg1Of	Specificity,was
R4470	T5344	T5346	arg2Of	Specificity,determined
R4471	T5346	T5345	arg2Of	determined,was
R4472	T5350	T5346	arg1Of	assay,determined
R4473	T5350	T5347	arg2Of	assay,by
R4474	T5350	T5348	arg1Of	assay,a
R4475	T5350	T5349	arg1Of	assay,competition
R4476	T5350	T5351	arg1Of	assay,with
R4477	T5353	T5351	arg2Of	addition,with
R4478	T5353	T5352	arg1Of	addition,the
R4479	T5353	T5354	arg1Of	addition,of
R4480	T5356	T5355	arg1Of	molar,200
R4481	T5357	T5354	arg2Of	excess,of
R4482	T5357	T5356	arg1Of	excess,molar
R4483	T5357	T5358	arg1Of	excess,of
R4484	T5361	T5359	arg1Of	stranded,unlabeled
R4485	T5361	T5360	arg1Of	stranded,double
R4486	T5363	T5358	arg2Of	oligonucleotide,of
R4487	T5363	T5361	arg1Of	oligonucleotide,stranded
R4488	T5363	T5362	arg1Of	oligonucleotide,CREB
R4489	T5365	T5364	arg1Of	extracts,Nuclear
R4490	T5365	T5366	arg1Of	extracts,(
R4491	T5365	T5370	arg1Of	extracts,were
R4492	T5365	T5371	arg2Of	extracts,incubated
R4493	T5368	T5366	arg2Of	μg,(
R4494	T5368	T5367	arg1Of	μg,10
R4495	T5369	T5366	arg3Of	),(
R4496	T5371	T5370	arg2Of	incubated,were
R4497	T5371	T5372	arg1Of	incubated,with
R4498	T5373	T5374	arg1Of	5,nM
R4499	T5375	T5373	arg1Of	NF-κB,5
R4500	T5375	T5376	arg1Of	NF-κB,or
R4501	T5376	T5372	arg2Of	or,with
R4502	T5378	T5376	arg2Of	probe,or
R4503	T5378	T5377	arg1Of	probe,CREB
R4504	T5378	T5379	arg1Of	probe,(
R4505	T5380	T5379	arg2Of	NF-kB,(
R4506	T5381	T5379	arg3Of	:,(
R4507	T5384	T5382	arg1Of	buffer,1x
R4508	T5384	T5383	arg1Of	buffer,binding
R4509	T5384	T5385	arg1Of	buffer,","
R4510	T5385	T5389	arg1Of	",",","
R4511	T5386	T5387	arg1Of	50,mM
R4512	T5388	T5385	arg2Of	KCl,","
R4513	T5388	T5386	arg1Of	KCl,50
R4514	T5389	T5393	arg1Of	",",","
R4515	T5390	T5391	arg1Of	1,mM
R4516	T5392	T5389	arg2Of	EDTA,","
R4517	T5392	T5390	arg1Of	EDTA,1
R4518	T5393	T5397	arg1Of	",",","
R4519	T5394	T5395	arg1Of	1,mM
R4520	T5396	T5393	arg2Of	DTT,","
R4521	T5396	T5394	arg1Of	DTT,1
R4522	T5397	T5401	arg1Of	",",","
R4523	T5398	T5399	arg1Of	0.1,%
R4524	T5400	T5397	arg2Of	NP40,","
R4525	T5400	T5398	arg1Of	NP40,0.1
R4526	T5401	T5406	arg1Of	",",and
R4527	T5403	T5402	arg1Of	%,10
R4528	T5404	T5401	arg2Of	glycerol,","
R4529	T5404	T5403	arg1Of	glycerol,%
R4530	T5406	T5405	arg1Of	and,","
R4531	T5410	T5406	arg2Of	dI-dC,and
R4532	T5410	T5407	arg1Of	dI-dC,50
R4533	T5410	T5408	arg1Of	dI-dC,ng/μl
R4534	T5410	T5409	arg1Of	dI-dC,poly
R4535	T5410	T5411	arg1Of	dI-dC,","
R4536	T5412	T5411	arg2Of	CREB,","
R4537	T5412	T5413	arg1Of	CREB,:
R4538	T5416	T5414	arg1Of	buffer,1x
R4539	T5416	T5415	arg1Of	buffer,binding
R4540	T5416	T5417	arg1Of	buffer,","
R4541	T5418	T5419	arg1Of	20,mM
R4542	T5420	T5417	arg2Of	Tris,","
R4543	T5420	T5418	arg1Of	Tris,20
R4544	T5420	T5421	arg1Of	Tris,","
R4545	T5422	T5421	arg2Of	pH,","
R4546	T5422	T5423	arg1Of	pH,7.5
R4547	T5425	T5426	arg1Of	50,mM
R4548	T5427	T5425	arg1Of	KCl,50
R4549	T5427	T5428	arg1Of	KCl,","
R4550	T5428	T5432	arg1Of	",",","
R4551	T5429	T5430	arg1Of	1,mM
R4552	T5431	T5428	arg2Of	EDTA,","
R4553	T5431	T5429	arg1Of	EDTA,1
R4554	T5433	T5434	arg1Of	1,mM
R4555	T5435	T5432	arg2Of	DTT,","
R4556	T5435	T5433	arg1Of	DTT,1
R4557	T5437	T5438	arg1Of	0.10,%
R4558	T5439	T5437	arg1Of	NP40,0.10
R4559	T5439	T5440	arg1Of	NP40,","
R4560	T5440	T5444	arg1Of	",",","
R4561	T5441	T5442	arg1Of	6,%
R4562	T5443	T5440	arg2Of	glycerol,","
R4563	T5443	T5441	arg1Of	glycerol,6
R4564	T5444	T5448	arg1Of	",",and
R4565	T5445	T5446	arg1Of	0.1,mg/mL
R4566	T5447	T5444	arg2Of	BSA,","
R4567	T5447	T5445	arg1Of	BSA,0.1
R4568	T5452	T5448	arg2Of	dI-dC,and
R4569	T5452	T5449	arg1Of	dI-dC,50
R4570	T5452	T5450	arg1Of	dI-dC,ng/μl
R4571	T5452	T5451	arg1Of	dI-dC,poly
R4572	T5457	T5454	arg2Of	electrophoresed,and
R4573	T5457	T5455	arg2Of	electrophoresed,were
R4574	T5457	T5456	arg1Of	electrophoresed,then
R4575	T5457	T5458	arg1Of	electrophoresed,through
R4576	T5460	T5461	arg1Of	6,%
R4577	T5464	T5458	arg2Of	gel,through
R4578	T5464	T5459	arg1Of	gel,a
R4579	T5464	T5460	arg1Of	gel,6
R4580	T5464	T5462	arg1Of	gel,DNA
R4581	T5464	T5463	arg1Of	gel,retardation
R4582	T5464	T5465	arg1Of	gel,at
R4583	T5466	T5465	arg2Of	100V,at
R4584	T5466	T5467	arg1Of	100V,for
R4585	T5469	T5467	arg2Of	minutes,for
R4586	T5469	T5468	arg1Of	minutes,90
R4587	T5471	T5470	arg1Of	gels,The
R4588	T5471	T5472	arg1Of	gels,were
R4589	T5471	T5474	arg2Of	gels,transferred
R4590	T5474	T5472	arg2Of	transferred,were
R4591	T5474	T5473	arg1Of	transferred,electrophoretically
R4592	T5474	T5475	arg1Of	transferred,at
R4593	T5476	T5475	arg2Of	380mA,at
R4594	T5476	T5477	arg1Of	380mA,for
R4595	T5476	T5489	arg1Of	380mA,immediately
R4596	T5477	T5488	arg1Of	for,and
R4597	T5479	T5477	arg2Of	hour,for
R4598	T5479	T5478	arg1Of	hour,1
R4599	T5479	T5480	arg1Of	hour,on
R4600	T5479	T5482	arg1Of	hour,to
R4601	T5481	T5480	arg2Of	ice,on
R4602	T5485	T5484	arg1Of	charged,positively
R4603	T5487	T5482	arg2Of	membrane,to
R4604	T5487	T5483	arg1Of	membrane,a
R4605	T5487	T5485	arg1Of	membrane,charged
R4606	T5487	T5486	arg1Of	membrane,nylon
R4607	T5489	T5488	arg2Of	immediately,and
R4608	T5489	T5490	arg1Of	immediately,cross-linked
R4609	T5490	T5491	arg1Of	cross-linked,for
R4610	T5493	T5491	arg2Of	minutes,for
R4611	T5493	T5492	arg1Of	minutes,15
R4612	T5493	T5494	arg1Of	minutes,with
R4613	T5497	T5494	arg2Of	transilluminator,with
R4614	T5497	T5495	arg1Of	transilluminator,a
R4615	T5497	T5496	arg1Of	transilluminator,UV
R4616	T5497	T5498	arg2Of	transilluminator,equipped
R4617	T5498	T5499	arg1Of	equipped,with
R4618	T5501	T5502	arg1Of	312,nm
R4619	T5503	T5499	arg2Of	bulb,with
R4620	T5503	T5500	arg1Of	bulb,a
R4621	T5503	T5501	arg1Of	bulb,312
R4622	T5506	T5504	arg1Of	conjugate,Streptavidin-horseradish
R4623	T5506	T5505	arg1Of	conjugate,peroxidase
R4624	T5506	T5507	arg1Of	conjugate,and
R4625	T5507	T5512	arg1Of	and,were
R4626	T5507	T5513	arg2Of	and,used
R4627	T5511	T5507	arg2Of	Substrate,and
R4628	T5511	T5508	arg1Of	Substrate,the
R4629	T5511	T5509	arg1Of	Substrate,LightShift
R4630	T5511	T5510	arg1Of	Substrate,Chemiluminescent
R4631	T5513	T5512	arg2Of	used,were
R4632	T5515	T5513	arg3Of	detect,used
R4633	T5515	T5514	arg1Of	detect,to
R4634	T5519	T5515	arg2Of	DNA,detect
R4635	T5519	T5516	arg1Of	DNA,the
R4636	T5519	T5517	arg1Of	DNA,biotin
R4637	T5519	T5518	arg1Of	DNA,end-labeled
R4638	T5522	T5520	arg1Of	membranes,The
R4639	T5522	T5521	arg1Of	membranes,nylon
R4640	T5522	T5523	arg1Of	membranes,were
R4641	T5522	T5524	arg2Of	membranes,exposed
R4642	T5524	T5523	arg2Of	exposed,were
R4643	T5524	T5525	arg1Of	exposed,to
R4644	T5524	T5528	arg1Of	exposed,for
R4645	T5527	T5525	arg2Of	film,to
R4646	T5527	T5526	arg1Of	film,x-ray
R4647	T5530	T5528	arg2Of	minutes,for
R4648	T5530	T5529	arg1Of	minutes,1–3
R4649	T5530	T5531	arg1Of	minutes,for
R4650	T5532	T5531	arg2Of	detection,for
R4831	T5799	T5800	arg1Of	Role,of
R4832	T5799	T5802	arg1Of	Role,on
R4833	T5801	T5800	arg2Of	PKA,of
R4834	T5807	T5802	arg2Of	production,on
R4835	T5807	T5803	arg1Of	production,LPS-induced
R4836	T5807	T5804	arg1Of	production,mononuclear
R4837	T5807	T5805	arg1Of	production,cell
R4838	T5807	T5806	arg1Of	production,TNF-α
R4839	T5809	T5808	arg1Of	evaluate,To
R4840	T5809	T5842	arg1Of	evaluate,was
R4841	T5809	T5843	arg2Of	evaluate,utilized
R4842	T5811	T5810	arg1Of	role,the
R4843	T5811	T5812	arg1Of	role,of
R4844	T5811	T5814	arg1Of	role,on
R4845	T5811	T5817	arg1Of	role,in
R4846	T5811	T5820	arg1Of	role,and
R4847	T5813	T5812	arg2Of	PKA,of
R4848	T5816	T5814	arg2Of	production,on
R4849	T5816	T5815	arg1Of	production,TNF-α
R4850	T5819	T5817	arg2Of	cells,in
R4851	T5819	T5818	arg1Of	cells,mononuclear
R4852	T5820	T5809	arg2Of	and,evaluate
R4853	T5822	T5820	arg2Of	involvement,and
R4854	T5822	T5821	arg1Of	involvement,its
R4855	T5822	T5823	arg1Of	involvement,in
R4856	T5825	T5823	arg2Of	attenuation,in
R4857	T5825	T5824	arg1Of	attenuation,the
R4858	T5825	T5826	arg1Of	attenuation,of
R4859	T5829	T5826	arg2Of	production,of
R4860	T5829	T5827	arg1Of	production,LPS-induced
R4861	T5829	T5828	arg1Of	production,TNF-α
R4862	T5829	T5830	arg2Of	production,observed
R4863	T5830	T5831	arg1Of	observed,following
R4864	T5833	T5831	arg2Of	treatment,following
R4865	T5833	T5832	arg1Of	treatment,PTX
R4866	T5833	T5834	arg1Of	treatment,","
R4867	T5833	T5836	arg1Of	treatment,","
R4868	T5835	T5834	arg2Of	H89,","
R4869	T5840	T5836	arg2Of	inhibitor,","
R4870	T5840	T5837	arg1Of	inhibitor,a
R4871	T5840	T5838	arg1Of	inhibitor,specific
R4872	T5840	T5839	arg1Of	inhibitor,PKA
R4873	T5843	T5841	arg1Of	utilized,","
R4874	T5843	T5842	arg2Of	utilized,was
R4875	T5846	T5844	arg2Of	cells,Isolated
R4876	T5846	T5845	arg1Of	cells,mononuclear
R4877	T5846	T5847	arg1Of	cells,were
R4878	T5846	T5848	arg2Of	cells,incubated
R4879	T5848	T5847	arg2Of	incubated,were
R4880	T5848	T5849	arg1Of	incubated,according
R4881	T5850	T5849	arg2Of	to,according
R4882	T5853	T5850	arg2Of	groups,to
R4883	T5853	T5851	arg1Of	groups,the
R4884	T5853	T5852	arg1Of	groups,treatment
R4885	T5853	T5854	arg2Of	groups,described
R4886	T5854	T5855	arg1Of	described,above
R4887	T5854	T5856	arg1Of	described,in
R4888	T5858	T5859	arg1Of	presence,and
R4889	T5859	T5856	arg2Of	and,in
R4890	T5859	T5857	arg1Of	and,the
R4891	T5859	T5861	arg1Of	and,of
R4892	T5859	T5863	arg1Of	and,with
R4893	T5860	T5859	arg2Of	absence,and
R4894	T5862	T5861	arg2Of	pretreatment,of
R4895	T5864	T5863	arg2Of	H89,with
R4896	T5864	T5865	arg1Of	H89,(
R4897	T5864	T5869	arg1Of	H89,for
R4898	T5867	T5865	arg2Of	μM,(
R4899	T5867	T5866	arg1Of	μM,10
R4900	T5868	T5865	arg3Of	),(
R4901	T5871	T5869	arg2Of	hour,for
R4902	T5871	T5870	arg1Of	hour,1
R4903	T5871	T5872	arg1Of	hour,at
R4904	T5873	T5872	arg2Of	37ºC,at
R4905	T5875	T5874	arg1Of	dose,The
R4906	T5875	T5876	arg1Of	dose,of
R4907	T5875	T5878	arg1Of	dose,was
R4908	T5875	T5879	arg2Of	dose,chosen
R4909	T5877	T5876	arg2Of	H89,of
R4910	T5879	T5878	arg2Of	chosen,was
R4911	T5879	T5880	modOf	chosen,based
R4912	T5879	T5881	arg1Of	chosen,on
R4913	T5883	T5881	arg2Of	work,on
R4914	T5883	T5882	arg1Of	work,previous
R4915	T5883	T5884	arg1Of	work,which
R4916	T5883	T5885	arg1Of	work,demonstrated
R4917	T5886	T5887	arg1Of	specific,and
R4918	T5888	T5887	arg2Of	complete,and
R4919	T5889	T5885	arg2Of	inhibition,demonstrated
R4920	T5889	T5886	arg1Of	inhibition,specific
R4921	T5889	T5888	arg1Of	inhibition,complete
R4922	T5889	T5890	arg1Of	inhibition,of
R4923	T5891	T5890	arg2Of	PKA,of
R4924	T5894	T5892	arg2Of	concentration.10,at
R4925	T5894	T5893	arg1Of	concentration.10,this
R4926	T5894	T5895	arg1Of	concentration.10,Since
R4927	T5897	T5896	arg1Of	activity,the
R4928	T5897	T5898	arg1Of	activity,of
R4929	T5897	T5901	arg1Of	activity,is
R4930	T5897	T5903	arg1Of	activity,specific
R4931	T5897	T5905	arg1Of	activity,consistent
R4932	T5900	T5898	arg2Of	inhibitor,of
R4933	T5900	T5899	arg1Of	inhibitor,this
R4934	T5901	T5895	arg2Of	is,Since
R4935	T5903	T5904	arg1Of	specific,and
R4936	T5904	T5901	arg2Of	and,is
R4937	T5904	T5902	arg1Of	and,both
R4938	T5904	T5906	arg1Of	and,at
R4939	T5905	T5904	arg2Of	consistent,and
R4940	T5909	T5906	arg2Of	dose,at
R4941	T5909	T5907	arg1Of	dose,this
R4942	T5909	T5908	arg2Of	dose,specified
R4943	T5911	T5912	arg1Of	we,did
R4944	T5911	T5914	arg1Of	we,include
R4945	T5914	T5885	arg3Of	include,demonstrated
R4946	T5914	T5892	arg1Of	include,at
R4947	T5914	T5910	arg1Of	include,","
R4948	T5914	T5912	arg2Of	include,did
R4949	T5914	T5913	arg1Of	include,not
R4950	T5915	T5914	arg2Of	experiments,include
R4951	T5915	T5916	arg1Of	experiments,with
R4952	T5919	T5916	arg2Of	inhibitors,with
R4953	T5919	T5917	arg1Of	inhibitors,additional
R4954	T5919	T5918	arg1Of	inhibitors,PKA
R4955	T5919	T5920	arg1Of	inhibitors,with
R4956	T5922	T5920	arg2Of	set,with
R4957	T5922	T5921	arg1Of	set,this
R4958	T5922	T5923	arg1Of	set,of
R4959	T5924	T5923	arg2Of	experiments,of
R4960	T5927	T5925	arg1Of	concentration,The
R4961	T5927	T5926	arg1Of	concentration,TNF-α
R4962	T5927	T5928	arg1Of	concentration,in
R4963	T5927	T5931	arg1Of	concentration,was
R4964	T5927	T5932	arg2Of	concentration,measured
R4965	T5930	T5928	arg2Of	supernatant,in
R4966	T5930	T5929	arg1Of	supernatant,the
R4967	T5932	T5931	arg2Of	measured,was
R4968	T5932	T5933	arg1Of	measured,quantitatively
R4969	T5935	T5932	arg1Of	ELISA,measured
R4970	T5935	T5934	arg2Of	ELISA,by
R4971	T5937	T5936	arg1Of	results,The
R4972	T5937	T5938	arg1Of	results,are
R4973	T5937	T5939	arg2Of	results,expressed
R4974	T5939	T5938	arg2Of	expressed,are
R4975	T5939	T5940	arg1Of	expressed,in
R4976	T5941	T5940	arg2Of	pg/mL,in
R5103	T6141	T6140	arg1Of	Analysis,Statistical
R5104	T6144	T6142	arg1Of	results,The
R5105	T6144	T6143	arg1Of	results,experimental
R5106	T6144	T6145	arg2Of	results,obtained
R5107	T6144	T6149	arg1Of	results,were
R5108	T6144	T6150	arg2Of	results,derived
R5109	T6145	T6146	arg1Of	obtained,in
R5110	T6148	T6146	arg2Of	study,in
R5111	T6148	T6147	arg1Of	study,this
R5112	T6150	T6149	arg2Of	derived,were
R5113	T6150	T6151	arg1Of	derived,from
R5114	T6154	T6151	arg2Of	experiments,from
R5115	T6154	T6152	arg1Of	experiments,four
R5116	T6154	T6153	arg1Of	experiments,separate
R5117	T6154	T6155	arg1Of	experiments,with
R5118	T6158	T6155	arg2Of	donors,with
R5119	T6158	T6156	arg1Of	donors,healthy
R5120	T6158	T6157	arg1Of	donors,volunteer
R5121	T6160	T6159	arg1Of	assay,Each
R5122	T6160	T6161	arg1Of	assay,was
R5123	T6160	T6162	arg2Of	assay,performed
R5124	T6160	T6166	arg1Of	assay,triplicate
R5125	T6162	T6161	arg2Of	performed,was
R5126	T6162	T6163	arg1Of	performed,in
R5127	T6162	T6165	arg1Of	performed,or
R5128	T6164	T6163	arg2Of	duplicate,in
R5129	T6166	T6165	arg2Of	triplicate,or
R5130	T6166	T6167	arg1Of	triplicate,where
R5131	T6168	T6167	arg2Of	appropriate,where
R5132	T6169	T6170	arg1Of	Data,is
R5133	T6169	T6171	arg2Of	Data,presented
R5134	T6171	T6170	arg2Of	presented,is
R5135	T6171	T6172	arg1Of	presented,as
R5136	T6176	T6172	arg2Of	SEM,as
R5137	T6176	T6173	arg1Of	SEM,the
R5138	T6176	T6174	arg1Of	SEM,mean
R5139	T6176	T6175	arg1Of	SEM,±
R5140	T6178	T6177	arg1Of	differences,Statistical
R5141	T6178	T6179	arg1Of	differences,between
R5142	T6178	T6181	arg1Of	differences,were
R5143	T6178	T6182	arg2Of	differences,determined
R5144	T6180	T6179	arg2Of	groups,between
R5145	T6182	T6181	arg2Of	determined,were
R5146	T6185	T6182	arg1Of	analysis,determined
R5147	T6185	T6183	arg2Of	analysis,by
R5148	T6185	T6184	arg1Of	analysis,one-way
R5149	T6185	T6186	arg1Of	analysis,of
R5150	T6185	T6191	arg1Of	analysis,with
R5151	T6187	T6186	arg2Of	variance,of
R5152	T6187	T6188	arg1Of	variance,(
R5153	T6189	T6188	arg2Of	ANOVA,(
R5154	T6190	T6188	arg3Of	),(
R5155	T6194	T6191	arg2Of	correction,with
R5156	T6194	T6192	arg1Of	correction,a
R5157	T6194	T6193	arg1Of	correction,Bonferroni
R5158	T6196	T6195	arg1Of	significance,Statistical
R5159	T6196	T6197	arg1Of	significance,was
R5160	T6196	T6198	arg2Of	significance,defined
R5161	T6198	T6197	arg2Of	defined,was
R5162	T6198	T6199	arg1Of	defined,as
R5163	T6202	T6199	arg2Of	0.05,as
R5164	T6202	T6200	arg1Of	0.05,P
R5165	T6202	T6201	arg1Of	0.05,<
R3312	T4034	T4035	arg1Of	concentration,of
R3313	T4034	T4037	arg1Of	concentration,was
R3314	T4034	T4039	arg2Of	concentration,determined
R3315	T4036	T4035	arg2Of	TNF-α,of
R3316	T4039	T4037	arg2Of	determined,was
R3317	T4039	T4038	arg1Of	determined,quantitatively
R3318	T4041	T4039	arg1Of	ELISA,determined
R3319	T4041	T4040	arg2Of	ELISA,by
R3320	T4041	T4042	arg1Of	ELISA,in
R3321	T4044	T4042	arg2Of	media,in
R3322	T4044	T4043	arg1Of	media,the

bionlp-st-ge-2016-spacy-parsed

Id	Subject	Object	Predicate	Lexical cue
T2467	37-45	VBN	denotes	approved
T2468	46-48	IN	denotes	by
T2469	49-52	DT	denotes	the
T2470	53-58	NNP	denotes	Human
T2471	59-67	NNP	denotes	Research
T2472	68-79	NNPS	denotes	Protections
T2473	80-87	NNP	denotes	Program
T2474	88-91	CC	denotes	and
T2475	92-95	DT	denotes	the
T2476	96-109	JJ	denotes	Institutional
T2477	110-116	NNP	denotes	Review
T2478	117-122	NNP	denotes	Board
T2479	122-123	.	denotes	.
T2480	124-131	VBN	denotes	Written
T2481	132-139	NN	denotes	consent
T2482	140-142	TO	denotes	to
T2483	143-154	VB	denotes	participate
T2484	155-157	IN	denotes	in
T2485	158-161	DT	denotes	the
T2486	162-167	NN	denotes	study
T2487	168-171	VBD	denotes	was
T2488	172-180	VBN	denotes	obtained
T2489	181-185	IN	denotes	from
T2490	186-189	DT	denotes	all
T2491	190-200	NNS	denotes	volunteers
T2492	201-206	RB	denotes	prior
T2493	207-209	TO	denotes	to
T2494	210-215	NN	denotes	blood
T2495	216-237	VBN	denotes	donation. Heparinized
T2496	238-249	NNS	denotes	Vacutainers
T2497	250-254	VBD	denotes	were
T2498	255-264	VBN	denotes	purchased
T2499	265-269	IN	denotes	from
T2500	270-276	NNP	denotes	Becton
T2501	277-286	NNP	denotes	Dickinson
T2502	287-288	-LRB-	denotes	(
T2503	288-291	NNP	denotes	San
T2504	292-296	NNP	denotes	Jose
T2505	296-297	,	denotes	,
T2506	298-300	NNP	denotes	CA
T2507	300-301	-RRB-	denotes	)
T2508	301-302	.	denotes	.
T2509	303-309	CD	denotes	1.5-mL
T2510	310-319	NNP	denotes	Eppendorf
T2511	320-330	NN	denotes	centrifuge
T2512	331-336	NNS	denotes	tubes
T2513	337-341	VBD	denotes	were
T2514	342-351	VBN	denotes	purchased
T2515	352-356	IN	denotes	from
T2516	357-363	NNP	denotes	Fisher
T2517	364-374	NNP	denotes	Scientific
T2518	375-376	-LRB-	denotes	(
T2519	376-385	NNP	denotes	Pittsburg
T2520	385-386	,	denotes	,
T2521	387-389	NNP	denotes	PA
T2522	389-390	-RRB-	denotes	)
T2523	390-391	.	denotes	.
T2524	392-396	NNP	denotes	Hank
T2525	399-407	JJ	denotes	balanced
T2526	408-412	NN	denotes	salt
T2527	413-421	NN	denotes	solution
T2528	422-423	-LRB-	denotes	(
T2529	423-427	NNP	denotes	HBSS
T2530	427-428	-RRB-	denotes	)
T2531	429-432	CC	denotes	and
T2532	433-437	NNP	denotes	RPMI
T2533	438-442	CD	denotes	1640
T2534	443-447	VBD	denotes	were
T2535	448-456	VBN	denotes	obtained
T2536	457-461	IN	denotes	from
T2537	462-468	NNP	denotes	Irvine
T2538	469-479	NNP	denotes	Scientific
T2539	480-481	-LRB-	denotes	(
T2540	481-486	NNP	denotes	Santa
T2541	487-490	NNP	denotes	Ana
T2542	490-491	,	denotes	,
T2543	492-494	NNP	denotes	CA
T2544	494-495	-RRB-	denotes	)
T2545	495-496	.	denotes	.
T2546	497-500	NNP	denotes	LPS
T2547	501-517	NNP	denotes	from Escherichia
T2548	518-531	NN	denotes	coli serotype
T2549	532-536	CD	denotes	0111
T2550	536-537	:	denotes	:
T2551	537-539	NN	denotes	B4
T2552	540-543	CC	denotes	and
T2553	544-547	NNP	denotes	PTX
T2554	548-552	VBD	denotes	were
T2555	553-562	VBN	denotes	purchased
T2556	563-567	IN	denotes	from
T2557	568-573	NNP	denotes	Sigma
T2558	574-575	-LRB-	denotes	(
T2559	575-578	NNP	denotes	St.
T2560	579-584	NNP	denotes	Louis
T2561	584-585	,	denotes	,
T2562	586-588	NNP	denotes	MO
T2563	588-589	-RRB-	denotes	)
T2564	589-590	.	denotes	.
T2565	591-594	DT	denotes	The
T2566	595-598	NNP	denotes	PKA
T2567	599-608	NN	denotes	inhibitor
T2568	608-609	,	denotes	,
T2569	610-611	NNP	denotes	N
T2570	611-612	:	denotes	-
T2571	612-613	NN	denotes	[
T2572	613-614	LS	denotes	2
T2573	614-615	:	denotes	-
T2574	615-616	-LRB-	denotes	(
T2575	616-617	-LRB-	denotes	(
T2576	617-632	NN	denotes	p-bromocinnamyl
T2577	632-633	-RRB-	denotes	)
T2578	633-638	JJ	denotes	amino
T2579	638-639	-RRB-	denotes	)
T2580	639-644	FW	denotes	ethyl
T2581	644-645	NNP	denotes	]
T2582	645-647	SYM	denotes	-5
T2583	647-648	:	denotes	-
T2584	648-676	NN	denotes	isoguinolinesulfonamide-2HCl
T2585	676-677	NNP	denotes	]
T2586	678-679	-LRB-	denotes	(
T2587	679-682	NNP	denotes	H89
T2588	682-683	-RRB-	denotes	)
T2589	683-684	,	denotes	,
T2590	685-688	VBD	denotes	was
T2591	689-698	VBN	denotes	purchased
T2592	699-703	IN	denotes	from
T2593	704-714	NNP	denotes	Calbiochem
T2594	715-716	-LRB-	denotes	(
T2595	716-718	NNP	denotes	La
T2596	719-724	NNP	denotes	Jolla
T2597	724-725	,	denotes	,
T2598	726-728	NNP	denotes	CA
T2599	728-729	-RRB-	denotes	)
T2600	729-730	.	denotes	.
T2601	731-741	NNS	denotes	Antibodies
T2602	742-745	IN	denotes	for
T2603	746-760	VBN	denotes	phosphorylated
T2604	761-765	NNP	denotes	CREB
T2605	766-767	-LRB-	denotes	(
T2606	767-773	NN	denotes	serine
T2607	774-777	CD	denotes	133
T2608	777-778	-RRB-	denotes	)
T2609	778-779	,	denotes	,
T2610	780-794	VBN	denotes	phosphorylated
T2611	795-800	NNP	denotes	I-κBα
T2612	801-802	-LRB-	denotes	(
T2613	802-809	NNS	denotes	serines
T2614	810-812	CD	denotes	32
T2615	813-816	CC	denotes	and
T2616	817-819	CD	denotes	36
T2617	819-820	-RRB-	denotes	)
T2618	820-821	,	denotes	,
T2619	822-836	VBN	denotes	phosphorylated
T2620	837-842	NNP	denotes	NF-κB
T2621	843-846	CD	denotes	p65
T2622	847-854	NN	denotes	subunit
T2623	855-856	-LRB-	denotes	(
T2624	856-862	NN	denotes	serine
T2625	863-866	CD	denotes	276
T2626	866-867	-RRB-	denotes	)
T2627	867-868	,	denotes	,
T2628	869-872	CC	denotes	and
T2629	873-882	JJ	denotes	secondary
T2630	883-893	NNS	denotes	antibodies
T2631	894-898	VBD	denotes	were
T2632	899-908	VBN	denotes	purchased
T2633	909-913	IN	denotes	from
T2634	914-918	NNP	denotes	Cell
T2635	919-928	NNP	denotes	Signaling
T2636	929-930	-LRB-	denotes	(
T2637	930-937	NNP	denotes	Beverly
T2638	937-938	,	denotes	,
T2639	939-941	NNP	denotes	MA
T2640	941-942	-RRB-	denotes	)
T2641	942-943	.	denotes	.
T2642	944-951	JJ	denotes	Dextran
T2643	952-956	CD	denotes	T500
T2644	957-960	CC	denotes	and
T2645	961-968	NNP	denotes	Percoll
T2646	969-973	VBD	denotes	were
T2647	974-982	VBN	denotes	received
T2648	983-987	IN	denotes	from
T2649	988-990	NNP	denotes	GE
T2650	991-1010	NNP	denotes	Healthcare/Amersham
T2651	1011-1022	NNP	denotes	Biosciences
T2652	1023-1024	-LRB-	denotes	(
T2653	1024-1034	NNP	denotes	Piscataway
T2654	1034-1035	,	denotes	,
T2655	1036-1038	NNP	denotes	NJ
T2656	1038-1039	-RRB-	denotes	)
T2657	1039-1040	.	denotes	.
T2658	1041-1044	DT	denotes	The
T2659	1045-1058	JJ	denotes	enzyme-linked
T2660	1059-1072	JJ	denotes	immunosorbent
T2661	1073-1078	NN	denotes	assay
T2662	1079-1080	-LRB-	denotes	(
T2663	1080-1085	NNP	denotes	ELISA
T2664	1085-1086	-RRB-	denotes	)
T2665	1087-1090	IN	denotes	for
T2666	1091-1096	NNP	denotes	TNF-α
T2667	1097-1100	VBD	denotes	was
T2668	1101-1109	VBN	denotes	obtained
T2669	1110-1114	IN	denotes	from
T2670	1115-1125	NNP	denotes	Quantikine
T2671	1126-1129	NNP	denotes	R&D
T2672	1130-1137	NNP	denotes	Systems
T2673	1138-1139	-LRB-	denotes	(
T2674	1139-1150	NNP	denotes	Minneapolis
T2675	1150-1151	,	denotes	,
T2676	1152-1154	NNP	denotes	MN
T2677	1154-1155	-RRB-	denotes	)
T2678	1155-1156	.	denotes	.
T2679	1157-1169	CD	denotes	Tris-glycine
T2680	1170-1173	CC	denotes	and
T2681	1174-1177	NNP	denotes	DNA
T2682	1178-1189	NN	denotes	retardation
T2683	1190-1194	NNS	denotes	gels
T2684	1194-1195	,	denotes	,
T2685	1196-1210	JJ	denotes	nitrocellulose
T2686	1211-1220	NNS	denotes	membranes
T2687	1220-1221	,	denotes	,
T2688	1222-1225	CC	denotes	and
T2689	1226-1233	JJ	denotes	western
T2690	1234-1238	NN	denotes	blot
T2691	1239-1246	VBG	denotes	running
T2692	1247-1254	NNS	denotes	buffers
T2693	1255-1259	VBD	denotes	were
T2694	1260-1268	VBN	denotes	obtained
T2695	1269-1273	IN	denotes	from
T2696	1274-1284	NNP	denotes	Invitrogen
T2697	1285-1286	-LRB-	denotes	(
T2698	1286-1294	NNP	denotes	Carlsbad
T2699	1294-1295	,	denotes	,
T2700	1296-1298	NNP	denotes	CA
T2701	1298-1299	-RRB-	denotes	)
T2702	1299-1300	.	denotes	.
T2703	1301-1307	NNP	denotes	NE-PER
T2704	1307-1308	NNP	denotes	®
T2705	1309-1316	JJ	denotes	nuclear
T2706	1317-1320	CC	denotes	and
T2707	1321-1332	JJ	denotes	cytoplasmic
T2708	1333-1343	NN	denotes	extraction
T2709	1344-1352	NNS	denotes	reagents
T2710	1352-1353	,	denotes	,
T2711	1354-1357	DT	denotes	the
T2712	1358-1361	NNP	denotes	BCA
T2713	1362-1369	NN	denotes	protein
T2714	1370-1377	NN	denotes	reagent
T2715	1378-1381	NN	denotes	kit
T2716	1381-1382	,	denotes	,
T2717	1383-1386	DT	denotes	the
T2718	1387-1398	NNP	denotes	Supersignal
T2719	1399-1403	NNP	denotes	West
T2720	1404-1408	NNP	denotes	Pico
T2721	1409-1425	NNP	denotes	Chemiluminescent
T2722	1426-1429	NNP	denotes	Kit
T2723	1429-1430	,	denotes	,
T2724	1431-1434	CC	denotes	and
T2725	1435-1438	DT	denotes	the
T2726	1439-1449	NNP	denotes	LightShift
T2727	1450-1466	NNP	denotes	Chemiluminescent
T2728	1467-1471	NNP	denotes	EMSA
T2729	1472-1475	NN	denotes	Kit
T2730	1476-1480	VBD	denotes	were
T2731	1481-1490	VBN	denotes	purchased
T2732	1491-1495	IN	denotes	from
T2733	1496-1502	NNP	denotes	Pierce
T2734	1503-1504	-LRB-	denotes	(
T2735	1504-1512	NNP	denotes	Rockland
T2736	1512-1513	,	denotes	,
T2737	1514-1516	NNP	denotes	IL
T2738	1516-1517	-RRB-	denotes	)
T2739	1517-1518	.	denotes	.
T2740	1519-1523	NNP	denotes	CREB
T2741	1524-1527	CC	denotes	and
T2742	1528-1533	NNP	denotes	NF-κB
T2743	1534-1537	NNP	denotes	DNA
T2744	1538-1553	NN	denotes	oligonucleotide
T2745	1554-1560	NNS	denotes	probes
T2746	1561-1565	VBD	denotes	were
T2747	1566-1577	VBN	denotes	synthesized
T2748	1578-1580	IN	denotes	by
T2749	1581-1584	NNP	denotes	IDT
T2750	1585-1586	-LRB-	denotes	(
T2751	1586-1596	NNP	denotes	Coralville
T2752	1596-1597	,	denotes	,
T2753	1598-1600	NNP	denotes	IA
T2754	1600-1601	-RRB-	denotes	)
T2755	1601-1602	.	denotes	.
T2756	1603-1608	NNP	denotes	Nylon
T2757	1609-1618	NNS	denotes	membranes
T2758	1619-1623	VBD	denotes	were
T2759	1624-1633	VBN	denotes	purchased
T2760	1634-1638	IN	denotes	from
T2761	1639-1644	NNP	denotes	Roche
T2762	1645-1652	NNP	denotes	Applied
T2763	1653-1661	NNP	denotes	Sciences
T2764	1662-1663	-LRB-	denotes	(
T2765	1663-1675	NNP	denotes	Indianapolis
T2766	1675-1676	,	denotes	,
T2767	1677-1679	IN	denotes	IN
T2768	1679-1680	-RRB-	denotes	)
T2769	1680-1681	.	denotes	.
T2770	1682-1689	JJ	denotes	Western
T2771	1690-1694	NN	denotes	blot
T2772	1695-1699	NN	denotes	band
T2773	1700-1714	NN	denotes	quantification
T2774	1715-1718	VBD	denotes	was
T2775	1719-1728	VBN	denotes	performed
T2776	1729-1733	IN	denotes	with
T2777	1734-1737	DT	denotes	the
T2778	1738-1748	NNP	denotes	UN-SCAN-IT
T2779	1749-1752	NNP	denotes	Gel
T2780	1753-1763	NNP	denotes	Digitizing
T2781	1764-1772	NN	denotes	software
T2782	1773-1774	-LRB-	denotes	(
T2783	1774-1778	NNP	denotes	Silk
T2784	1779-1789	NNP	denotes	Scientific
T2785	1789-1790	,	denotes	,
T2786	1791-1795	NNP	denotes	Orem
T2787	1795-1796	,	denotes	,
T2788	1797-1799	NNP	denotes	UT
T2789	1799-1800	-RRB-	denotes	)
T2790	1800-1801	.	denotes	.
T3267	1803-1808	NNP	denotes	Human
T3268	1809-1819	NNP	denotes	Peripheral
T3269	1820-1825	NNP	denotes	Blood
T3270	1826-1837	NNP	denotes	Mononuclear
T3271	1838-1842	NNP	denotes	Cell
T3272	1843-1852	NNP	denotes	Isolation
T3273	1853-1856	CC	denotes	and
T3274	1857-1868	NNP	denotes	Stimulation
T3275	1869-1874	NNP	denotes	Human
T3276	1875-1886	NN	denotes	mononuclear
T3277	1887-1892	NNS	denotes	cells
T3278	1893-1897	VBD	denotes	were
T3279	1898-1906	VBN	denotes	isolated
T3280	1907-1911	IN	denotes	from
T3281	1912-1915	DT	denotes	the
T3282	1916-1926	JJ	denotes	peripheral
T3283	1927-1932	NN	denotes	blood
T3284	1933-1935	IN	denotes	of
T3285	1936-1940	CD	denotes	four
T3286	1941-1948	JJ	denotes	healthy
T3287	1949-1954	JJ	denotes	human
T3288	1955-1965	NNS	denotes	volunteers
T3289	1965-1966	.	denotes	.
T3290	1967-1970	DT	denotes	The
T3291	1971-1977	NN	denotes	sample
T3292	1978-1982	NN	denotes	size
T3293	1983-1986	VBD	denotes	was
T3294	1987-1993	VBN	denotes	chosen
T3295	1994-1998	IN	denotes	from
T3296	1999-2002	PRP$	denotes	our
T3297	2003-2013	RB	denotes	previously
T3298	2014-2023	VBN	denotes	published
T3299	2024-2028	NN	denotes	work
T3300	2028-2029	,	denotes	,
T3301	2030-2035	WDT	denotes	which
T3302	2036-2048	VBD	denotes	demonstrated
T3303	2049-2057	JJ	denotes	reliable
T3304	2058-2065	NNS	denotes	results
T3305	2065-2068	CD	denotes	.10
T3306	2069-2073	NNP	denotes	Cell
T3307	2074-2083	NN	denotes	isolation
T3308	2084-2087	CC	denotes	and
T3309	2088-2091	DT	denotes	all
T3310	2092-2102	JJ	denotes	subsequent
T3311	2103-2114	NNS	denotes	experiments
T3312	2115-2119	VBD	denotes	were
T3313	2120-2129	VBN	denotes	conducted
T3314	2130-2135	IN	denotes	under
T3315	2136-2143	JJ	denotes	sterile
T3316	2144-2147	CC	denotes	and
T3317	2148-2160	JJ	denotes	pyrogen-free
T3318	2161-2171	NNS	denotes	conditions
T3319	2171-2172	.	denotes	.
T3320	2173-2178	NNP	denotes	Blood
T3321	2179-2188	VBN	denotes	collected
T3322	2189-2191	IN	denotes	in
T3323	2192-2199	NN	denotes	heparin
T3324	2200-2205	NNS	denotes	tubes
T3325	2206-2209	VBD	denotes	was
T3326	2210-2219	VBN	denotes	incubated
T3327	2220-2224	IN	denotes	with
T3328	2225-2232	NNP	denotes	Dextran
T3329	2233-2237	CD	denotes	T500
T3330	2238-2241	CC	denotes	and
T3331	2242-2245	DT	denotes	the
T3332	2246-2249	JJ	denotes	red
T3333	2250-2255	NNS	denotes	cells
T3334	2256-2260	VBD	denotes	were
T3335	2261-2271	VBN	denotes	sedimented
T3336	2272-2275	IN	denotes	for
T3337	2276-2278	CD	denotes	40
T3338	2279-2286	NNS	denotes	minutes
T3339	2287-2289	IN	denotes	at
T3340	2290-2294	NN	denotes	room
T3341	2295-2306	NN	denotes	temperature
T3342	2306-2307	.	denotes	.
T3343	2308-2311	DT	denotes	The
T3344	2312-2321	JJ	denotes	resultant
T3345	2322-2327	NN	denotes	serum
T3346	2328-2331	VBD	denotes	was
T3347	2332-2336	RB	denotes	then
T3348	2337-2343	VBN	denotes	washed
T3349	2344-2348	IN	denotes	with
T3350	2349-2353	NNP	denotes	HBSS
T3351	2354-2357	CC	denotes	and
T3352	2358-2367	VBN	denotes	separated
T3353	2368-2370	IN	denotes	by
T3354	2371-2378	NNP	denotes	Percoll
T3355	2379-2387	NN	denotes	gradient
T3356	2388-2402	NN	denotes	centrifugation
T3357	2403-2412	VBG	denotes	according
T3358	2413-2415	TO	denotes	to
T3359	2416-2419	DT	denotes	the
T3360	2420-2432	NN	denotes	manufacturer
T3361	2435-2447	NNS	denotes	instructions
T3362	2447-2448	.	denotes	.
T3363	2449-2453	NNP	denotes	Cell
T3364	2454-2463	NN	denotes	viability
T3365	2464-2467	VBD	denotes	was
T3366	2468-2476	VBN	denotes	assessed
T3367	2477-2479	IN	denotes	by
T3368	2480-2486	NN	denotes	trypan
T3369	2487-2491	JJ	denotes	blue
T3370	2492-2495	NN	denotes	dye
T3371	2496-2505	NN	denotes	exclusion
T3372	2505-2506	,	denotes	,
T3373	2507-2511	IN	denotes	with
T3374	2512-2518	NN	denotes	purity
T3375	2519-2521	IN	denotes	of
T3376	2522-2529	JJR	denotes	greater
T3377	2530-2534	IN	denotes	than
T3378	2535-2537	CD	denotes	95
T3379	2537-2538	NN	denotes	%
T3380	2538-2539	.	denotes	.
T3381	2540-2548	VBN	denotes	Isolated
T3382	2549-2554	NNS	denotes	cells
T3383	2555-2559	VBD	denotes	were
T3384	2560-2571	VBN	denotes	resuspended
T3385	2572-2574	IN	denotes	in
T3386	2575-2579	NNP	denotes	RPMI
T3387	2580-2584	CD	denotes	1640
T3388	2585-2597	VBN	denotes	supplemented
T3389	2598-2602	IN	denotes	with
T3390	2603-2605	CD	denotes	10
T3391	2605-2606	NN	denotes	%
T3392	2607-2610	NNP	denotes	FBS
T3393	2611-2614	CC	denotes	and
T3394	2615-2616	CD	denotes	5
T3395	2617-2619	NNP	denotes	mM
T3396	2620-2625	NNP	denotes	HEPES
T3397	2626-2628	IN	denotes	at
T3398	2629-2630	DT	denotes	a
T3399	2631-2644	NN	denotes	concentration
T3400	2645-2647	IN	denotes	of
T3401	2648-2649	CD	denotes	1
T3402	2650-2651	LS	denotes	x
T3403	2652-2663	NNP	denotes	107cells/mL
T3404	2663-2664	.	denotes	.
T3405	2665-2669	DT	denotes	Each
T3406	2670-2680	JJ	denotes	subsequent
T3407	2681-2691	NN	denotes	experiment
T3408	2692-2698	VBN	denotes	listed
T3409	2699-2704	RB	denotes	below
T3410	2705-2708	VBD	denotes	was
T3411	2709-2718	VBN	denotes	conducted
T3412	2719-2721	IN	denotes	on
T3413	2722-2729	NNS	denotes	samples
T3414	2730-2732	IN	denotes	of
T3415	2733-2734	CD	denotes	5
T3416	2735-2736	LS	denotes	x
T3417	2737-2740	CD	denotes	106
T3418	2741-2746	NNS	denotes	cells
T3419	2747-2750	IN	denotes	per
T3420	2751-2760	NN	denotes	treatment
T3421	2761-2766	NN	denotes	group
T3422	2766-2767	.	denotes	.
T3423	2768-2776	VBN	denotes	Isolated
T3424	2777-2782	NNS	denotes	cells
T3425	2783-2787	VBD	denotes	were
T3426	2788-2798	VBN	denotes	stimulated
T3427	2799-2803	IN	denotes	with
T3428	2804-2810	DT	denotes	either
T3429	2811-2815	NNP	denotes	HBSS
T3430	2816-2818	IN	denotes	as
T3431	2819-2820	DT	denotes	a
T3432	2821-2829	JJ	denotes	negative
T3433	2830-2837	NN	denotes	control
T3434	2837-2838	,	denotes	,
T3435	2839-2842	NNP	denotes	LPS
T3436	2843-2844	-LRB-	denotes	(
T3437	2844-2845	CD	denotes	1
T3438	2846-2848	NN	denotes	μg
T3439	2848-2849	NN	denotes	/
T3440	2849-2851	NNP	denotes	mL
T3441	2851-2852	-RRB-	denotes	)
T3442	2852-2853	,	denotes	,
T3443	2854-2857	NNP	denotes	PTX
T3444	2858-2859	-LRB-	denotes	(
T3445	2859-2861	CD	denotes	20
T3446	2862-2864	NNP	denotes	mM
T3447	2864-2865	-RRB-	denotes	)
T3448	2865-2866	,	denotes	,
T3449	2867-2869	CC	denotes	or
T3450	2870-2881	JJ	denotes	concomitant
T3451	2882-2885	NNP	denotes	LPS
T3452	2886-2887	NN	denotes	/
T3453	2888-2891	NNP	denotes	PTX
T3454	2891-2892	,	denotes	,
T3455	2893-2895	IN	denotes	at
T3456	2896-2899	DT	denotes	the
T3457	2900-2915	JJ	denotes	above-mentioned
T3458	2916-2930	NNS	denotes	concentrations
T3459	2930-2931	,	denotes	,
T3460	2932-2935	IN	denotes	for
T3461	2936-2938	CD	denotes	30
T3462	2939-2946	NNS	denotes	minutes
T3463	2947-2949	IN	denotes	at
T3464	2950-2954	NNP	denotes	37ºC
T3465	2954-2955	.	denotes	.
T3466	2956-2961	NNS	denotes	Cells
T3467	2962-2966	VBD	denotes	were
T3468	2967-2973	VBN	denotes	placed
T3469	2974-2976	IN	denotes	on
T3470	2977-2980	NN	denotes	ice
T3471	2981-2984	IN	denotes	for
T3472	2985-2987	CD	denotes	10
T3473	2988-2995	NNS	denotes	minutes
T3474	2996-2998	TO	denotes	to
T3475	2999-3003	VB	denotes	stop
T3476	3004-3007	DT	denotes	the
T3477	3008-3016	NN	denotes	reaction
T3478	3017-3020	CC	denotes	and
T3479	3021-3024	DT	denotes	the
T3480	3025-3032	NNS	denotes	samples
T3481	3033-3037	VBD	denotes	were
T3482	3038-3042	RB	denotes	then
T3483	3043-3049	VBN	denotes	stored
T3484	3050-3052	IN	denotes	at
T3485	3053-3054	CD	denotes	−
T3486	3054-3056	CD	denotes	70
T3487	3056-3057	NN	denotes	°
T3488	3057-3058	NNP	denotes	C
T3489	3059-3062	IN	denotes	for
T3490	3063-3070	JJ	denotes	further
T3491	3071-3079	NN	denotes	analysis
T3492	3079-3080	.	denotes	.
T3493	3081-3084	DT	denotes	The
T3494	3085-3096	NN	denotes	stimulation
T3495	3097-3102	NNS	denotes	times
T3496	3103-3106	CC	denotes	and
T3497	3107-3121	NNS	denotes	concentrations
T3498	3122-3124	IN	denotes	of
T3499	3125-3128	NNP	denotes	LPS
T3500	3129-3132	CC	denotes	and
T3501	3133-3136	NNP	denotes	PTX
T3502	3137-3145	VBD	denotes	utilized
T3503	3146-3148	IN	denotes	in
T3504	3149-3153	DT	denotes	this
T3505	3154-3159	NN	denotes	study
T3506	3160-3164	VBD	denotes	were
T3507	3165-3175	VBN	denotes	determined
T3508	3176-3178	IN	denotes	in
T3509	3179-3187	JJ	denotes	previous
T3510	3188-3193	NN	denotes	pilot
T3511	3194-3201	NNS	denotes	studies
T3512	3202-3206	VBN	denotes	done
T3513	3207-3209	IN	denotes	in
T3514	3210-3213	PRP$	denotes	our
T3515	3214-3224	NN	denotes	laboratory
T3516	3224-3225	,	denotes	,
T3517	3226-3231	WDT	denotes	which
T3518	3232-3240	VBD	denotes	examined
T3519	3241-3244	DT	denotes	the
T3520	3245-3252	NNS	denotes	effects
T3521	3253-3255	IN	denotes	of
T3522	3256-3266	VBG	denotes	increasing
T3523	3267-3281	NNS	denotes	concentrations
T3524	3282-3284	IN	denotes	of
T3525	3285-3288	NNP	denotes	PTX
T3526	3289-3291	IN	denotes	on
T3527	3292-3303	JJ	denotes	LPS-induced
T3528	3304-3309	JJ	denotes	TNF-α
T3529	3310-3320	NN	denotes	production
T3530	3320-3321	.	denotes	.
T3531	3322-3325	NNP	denotes	LPS
T3532	3326-3328	IN	denotes	at
T3533	3329-3330	DT	denotes	a
T3534	3331-3344	NN	denotes	concentration
T3535	3345-3347	IN	denotes	of
T3536	3348-3349	CD	denotes	1
T3537	3350-3352	NN	denotes	μg
T3538	3352-3353	NN	denotes	/
T3539	3353-3355	NNP	denotes	mL
T3540	3356-3359	CC	denotes	and
T3541	3360-3363	NNP	denotes	PTX
T3542	3364-3366	IN	denotes	at
T3543	3367-3368	DT	denotes	a
T3544	3369-3382	NN	denotes	concentration
T3545	3383-3385	IN	denotes	of
T3546	3386-3388	CD	denotes	20
T3547	3389-3391	NN	denotes	mM
T3548	3392-3396	VBD	denotes	were
T3549	3397-3400	DT	denotes	the
T3550	3401-3408	JJ	denotes	minimum
T3551	3409-3423	NNS	denotes	concentrations
T3552	3424-3433	JJ	denotes	necessary
T3553	3434-3436	TO	denotes	to
T3554	3437-3444	VB	denotes	produce
T3555	3445-3446	DT	denotes	a
T3556	3447-3459	JJ	denotes	reproducible
T3557	3460-3463	CC	denotes	and
T3558	3464-3472	JJ	denotes	reliable
T3559	3473-3486	NN	denotes	up-regulation
T3560	3487-3490	CC	denotes	and
T3561	3491-3506	NN	denotes	down-regulation
T3562	3507-3509	IN	denotes	in
T3563	3510-3515	JJ	denotes	TNF-α
T3564	3516-3526	NN	denotes	production
T3565	3526-3527	,	denotes	,
T3566	3528-3540	RB	denotes	respectively
T3567	3540-3541	,	denotes	,
T3568	3542-3544	IN	denotes	in
T3569	3545-3557	JJ	denotes	quantitative
T3570	3558-3564	NNS	denotes	assays
T3571	3565-3566	-LRB-	denotes	(
T3572	3566-3570	NNS	denotes	data
T3573	3571-3574	RB	denotes	not
T3574	3575-3580	VBN	denotes	shown
T3575	3580-3581	-RRB-	denotes	)
T3576	3581-3582	.	denotes	.
T3971	3584-3590	NN	denotes	Effect
T3972	3591-3593	IN	denotes	of
T3973	3594-3597	NNP	denotes	PTX
T3974	3598-3600	IN	denotes	on
T3975	3601-3612	JJ	denotes	LPS-induced
T3976	3613-3618	JJ	denotes	TNF-α
T3977	3619-3629	NN	denotes	production
T3978	3630-3632	IN	denotes	by
T3979	3633-3644	NN	denotes	mononuclear
T3980	3645-3650	NNS	denotes	cells
T3981	3651-3654	DT	denotes	The
T3982	3655-3668	NN	denotes	concentration
T3983	3669-3671	IN	denotes	of
T3984	3672-3677	NNP	denotes	TNF-α
T3985	3678-3681	VBD	denotes	was
T3986	3682-3696	RB	denotes	quantitatively
T3987	3697-3707	VBN	denotes	determined
T3988	3708-3710	IN	denotes	by
T3989	3711-3716	NNP	denotes	ELISA
T3990	3717-3719	IN	denotes	in
T3991	3720-3723	DT	denotes	the
T3992	3724-3729	NNS	denotes	media
T3993	3730-3732	IN	denotes	of
T3994	3733-3738	NNS	denotes	cells
T3995	3739-3746	VBN	denotes	exposed
T3996	3747-3749	TO	denotes	to
T3997	3750-3753	DT	denotes	the
T3998	3754-3764	NNS	denotes	treatments
T3999	3765-3774	VBN	denotes	described
T4000	3775-3780	IN	denotes	above
T4001	3781-3786	IN	denotes	after
T4002	3787-3798	NN	denotes	stimulation
T4003	3799-3802	IN	denotes	for
T4004	3803-3805	RB	denotes	up
T4005	3806-3808	TO	denotes	to
T4006	3809-3810	DT	denotes	a
T4007	3811-3818	NN	denotes	maximum
T4008	3819-3821	IN	denotes	of
T4009	3822-3824	CD	denotes	18
T4010	3825-3830	NNS	denotes	hours
T4011	3830-3831	,	denotes	,
T4012	3832-3834	IN	denotes	at
T4013	3835-3841	JJ	denotes	3-hour
T4014	3842-3851	NNS	denotes	intervals
T4015	3851-3852	.	denotes	.
T4016	3853-3856	DT	denotes	The
T4017	3857-3864	NNS	denotes	results
T4018	3865-3868	VBP	denotes	are
T4019	3869-3878	VBN	denotes	expressed
T4020	3879-3881	IN	denotes	in
T4021	3882-3887	NNP	denotes	pg/mL
T4022	3887-3888	.	denotes	.
T4152	3890-3898	NNP	denotes	SDS-PAGE
T4153	3899-3902	CC	denotes	and
T4154	3903-3917	NNP	denotes	Immunoblotting
T4155	3918-3919	-LRB-	denotes	(
T4156	3919-3924	NNP	denotes	I-κBα
T4157	3924-3925	,	denotes	,
T4158	3926-3931	NNP	denotes	NF-κB
T4159	3932-3935	CD	denotes	p65
T4160	3935-3936	,	denotes	,
T4161	3937-3940	CC	denotes	and
T4162	3941-3945	NNP	denotes	CREB
T4163	3945-3946	-RRB-	denotes	)
T4164	3947-3956	VBG	denotes	Following
T4165	3957-3968	NN	denotes	stimulation
T4166	3968-3969	,	denotes	,
T4167	3970-3975	NNS	denotes	PBMCs
T4168	3976-3980	VBD	denotes	were
T4169	3981-3987	VBN	denotes	washed
T4170	3988-3992	IN	denotes	with
T4171	3993-4001	JJ	denotes	ice-cold
T4172	4002-4005	NNP	denotes	PBS
T4173	4006-4009	CC	denotes	and
T4174	4010-4021	VBD	denotes	centrifuged
T4175	4022-4024	TO	denotes	to
T4176	4025-4032	VB	denotes	collect
T4177	4033-4034	DT	denotes	a
T4178	4035-4039	NN	denotes	cell
T4179	4040-4046	NN	denotes	pellet
T4180	4046-4047	.	denotes	.
T4181	4048-4051	DT	denotes	The
T4182	4052-4058	NN	denotes	pellet
T4183	4059-4062	VBD	denotes	was
T4184	4063-4074	VBN	denotes	resuspended
T4185	4075-4077	IN	denotes	in
T4186	4078-4086	JJ	denotes	ice-cold
T4187	4087-4090	NNP	denotes	SDS
T4188	4091-4097	NN	denotes	sample
T4189	4098-4104	NN	denotes	buffer
T4190	4105-4117	VBN	denotes	supplemented
T4191	4118-4122	IN	denotes	with
T4192	4123-4126	CD	denotes	100
T4193	4127-4129	NNP	denotes	mM
T4194	4130-4144	NN	denotes	dithiothreitol
T4195	4144-4145	.	denotes	.
T4196	4146-4157	NNP	denotes	Cytoplasmic
T4197	4158-4161	CC	denotes	and
T4198	4162-4169	JJ	denotes	nuclear
T4199	4170-4178	NNS	denotes	extracts
T4200	4179-4183	VBD	denotes	were
T4201	4184-4192	VBN	denotes	isolated
T4202	4193-4197	IN	denotes	with
T4203	4198-4204	NNP	denotes	NE-PER
T4204	4205-4212	JJ	denotes	nuclear
T4205	4213-4216	CC	denotes	and
T4206	4217-4228	JJ	denotes	cytoplasmic
T4207	4229-4239	NN	denotes	extraction
T4208	4240-4248	VBZ	denotes	reagents
T4209	4249-4253	IN	denotes	with
T4210	4254-4256	CD	denotes	1x
T4211	4257-4261	NNP	denotes	Halt
T4212	4262-4270	NNP	denotes	Protease
T4213	4271-4280	NNP	denotes	Inhibitor
T4214	4281-4289	NNP	denotes	Cocktail
T4215	4290-4299	VBG	denotes	according
T4216	4300-4302	TO	denotes	to
T4217	4303-4306	DT	denotes	the
T4218	4307-4319	NN	denotes	manufacturer
T4219	4322-4334	NNS	denotes	instructions
T4220	4334-4335	.	denotes	.
T4221	4336-4343	NN	denotes	Protein
T4222	4344-4358	NNS	denotes	concentrations
T4223	4359-4363	VBD	denotes	were
T4224	4364-4374	VBN	denotes	determined
T4225	4375-4379	IN	denotes	with
T4226	4380-4383	DT	denotes	the
T4227	4384-4387	NNP	denotes	BCA
T4228	4388-4395	NN	denotes	protein
T4229	4396-4403	NN	denotes	reagent
T4230	4404-4407	NN	denotes	kit
T4231	4408-4411	IN	denotes	for
T4232	4412-4416	DT	denotes	each
T4233	4417-4423	NN	denotes	sample
T4234	4424-4433	VBG	denotes	according
T4235	4434-4436	TO	denotes	to
T4236	4437-4438	DT	denotes	a
T4237	4439-4451	JJ	denotes	standardized
T4238	4452-4457	NN	denotes	curve
T4239	4458-4461	IN	denotes	for
T4240	4462-4469	NN	denotes	albumin
T4241	4469-4470	.	denotes	.
T4242	4471-4475	NN	denotes	Cell
T4243	4476-4483	NNS	denotes	lysates
T4244	4484-4488	VBD	denotes	were
T4245	4489-4498	VBN	denotes	collected
T4246	4499-4501	IN	denotes	by
T4247	4502-4509	VBG	denotes	boiling
T4248	4510-4513	DT	denotes	the
T4249	4514-4521	NNS	denotes	samples
T4250	4522-4524	IN	denotes	in
T4251	4525-4526	DT	denotes	a
T4252	4527-4532	JJ	denotes	100ºC
T4253	4533-4538	NN	denotes	water
T4254	4539-4543	NN	denotes	bath
T4255	4544-4547	IN	denotes	for
T4256	4548-4549	CD	denotes	5
T4257	4550-4557	NNS	denotes	minutes
T4258	4557-4558	.	denotes	.
T4259	4559-4562	CD	denotes	Ten
T4260	4563-4565	NN	denotes	μg
T4261	4566-4568	IN	denotes	of
T4262	4569-4576	NN	denotes	protein
T4263	4577-4580	IN	denotes	per
T4264	4581-4587	NN	denotes	sample
T4265	4588-4591	VBD	denotes	was
T4266	4592-4601	VBN	denotes	separated
T4267	4602-4604	IN	denotes	by
T4268	4605-4623	JJ	denotes	SDS-polyacrylamide
T4269	4624-4627	NN	denotes	gel
T4270	4628-4643	NN	denotes	electrophoresis
T4271	4644-4651	IN	denotes	through
T4272	4652-4653	CD	denotes	8
T4273	4654-4656	CD	denotes	16
T4274	4656-4657	NN	denotes	%
T4275	4658-4670	JJ	denotes	tris-glycine
T4276	4671-4685	NN	denotes	polyacrylamide
T4277	4686-4694	NN	denotes	gradient
T4278	4695-4699	NNS	denotes	gels
T4279	4700-4703	CC	denotes	and
T4280	4704-4715	VBN	denotes	transferred
T4281	4716-4718	TO	denotes	to
T4282	4719-4733	JJ	denotes	nitrocellulose
T4283	4734-4743	NNS	denotes	membranes
T4284	4743-4744	.	denotes	.
T4285	4745-4748	DT	denotes	The
T4286	4749-4758	NNS	denotes	membranes
T4287	4759-4763	VBD	denotes	were
T4288	4764-4771	VBN	denotes	blocked
T4289	4772-4776	IN	denotes	with
T4290	4777-4778	CD	denotes	5
T4291	4778-4779	NN	denotes	%
T4292	4780-4784	NN	denotes	milk
T4293	4785-4787	IN	denotes	in
T4294	4788-4801	JJ	denotes	Tris-buffered
T4295	4802-4814	NNP	denotes	saline/Tween
T4296	4815-4817	CD	denotes	20
T4297	4818-4819	-LRB-	denotes	(
T4298	4819-4826	NNP	denotes	Fischer
T4299	4827-4837	NNP	denotes	Scientific
T4300	4837-4838	,	denotes	,
T4301	4839-4849	NNP	denotes	Pittsburgh
T4302	4849-4850	,	denotes	,
T4303	4851-4853	NNP	denotes	PA
T4304	4853-4854	-RRB-	denotes	)
T4305	4855-4858	IN	denotes	for
T4306	4859-4860	CD	denotes	1
T4307	4861-4865	NN	denotes	hour
T4308	4865-4866	.	denotes	.
T4309	4867-4878	JJ	denotes	Cytoplasmic
T4310	4879-4887	NNS	denotes	extracts
T4311	4888-4892	VBD	denotes	were
T4312	4893-4902	VBN	denotes	incubated
T4313	4903-4907	IN	denotes	with
T4314	4908-4922	JJ	denotes	phosphorylated
T4315	4923-4928	JJ	denotes	I-κBα
T4316	4929-4937	NN	denotes	antibody
T4317	4938-4939	-LRB-	denotes	(
T4318	4939-4944	CD	denotes	1:200
T4319	4944-4945	-RRB-	denotes	)
T4320	4945-4946	,	denotes	,
T4321	4947-4952	IN	denotes	while
T4322	4953-4960	JJ	denotes	nuclear
T4323	4961-4969	NNS	denotes	extracts
T4324	4970-4974	VBD	denotes	were
T4325	4975-4984	VBN	denotes	incubated
T4326	4985-4989	IN	denotes	with
T4327	4990-4996	DT	denotes	either
T4328	4997-5011	VBN	denotes	phosphorylated
T4329	5012-5017	NNP	denotes	NF-κB
T4330	5018-5021	CD	denotes	p65
T4331	5022-5030	NN	denotes	antibody
T4332	5031-5032	-LRB-	denotes	(
T4333	5032-5037	CD	denotes	1:500
T4334	5037-5038	-RRB-	denotes	)
T4335	5039-5041	CC	denotes	or
T4336	5042-5056	VBN	denotes	phosphorylated
T4337	5057-5061	NNP	denotes	CREB
T4338	5062-5070	NN	denotes	antibody
T4339	5071-5072	-LRB-	denotes	(
T4340	5072-5077	CD	denotes	1:500
T4341	5077-5078	-RRB-	denotes	)
T4342	5079-5088	RB	denotes	overnight
T4343	5089-5091	IN	denotes	at
T4344	5092-5093	CD	denotes	4
T4345	5093-5094	CD	denotes	°
T4346	5094-5095	NNP	denotes	C
T4347	5096-5098	IN	denotes	in
T4348	5099-5107	JJ	denotes	separate
T4349	5108-5119	NNS	denotes	experiments
T4350	5119-5120	.	denotes	.
T4351	5121-5124	DT	denotes	The
T4352	5125-5134	NNS	denotes	membranes
T4353	5135-5139	VBD	denotes	were
T4354	5140-5146	VBN	denotes	washed
T4355	5147-5151	IN	denotes	with
T4356	5152-5165	JJ	denotes	Tris-buffered
T4357	5166-5178	NNP	denotes	saline/Tween
T4358	5179-5181	CD	denotes	20
T4359	5182-5185	CC	denotes	and
T4360	5186-5195	VBD	denotes	incubated
T4361	5196-5199	IN	denotes	for
T4362	5200-5201	CD	denotes	1
T4363	5202-5206	NN	denotes	hour
T4364	5207-5209	IN	denotes	at
T4365	5210-5214	NN	denotes	room
T4366	5215-5226	NN	denotes	temperature
T4367	5227-5231	IN	denotes	with
T4368	5232-5235	DT	denotes	the
T4369	5236-5245	JJ	denotes	secondary
T4370	5246-5254	NN	denotes	antibody
T4371	5254-5255	,	denotes	,
T4372	5256-5267	JJ	denotes	horseradish
T4373	5268-5285	JJ	denotes	peroxidase-linked
T4374	5286-5297	JJ	denotes	anti-rabbit
T4375	5298-5301	NNP	denotes	IgG
T4376	5302-5309	VBD	denotes	diluted
T4377	5310-5316	CD	denotes	1:2000
T4378	5317-5319	IN	denotes	in
T4379	5320-5328	VBG	denotes	blocking
T4380	5329-5337	NN	denotes	solution
T4381	5337-5338	.	denotes	.
T4382	5339-5344	IN	denotes	After
T4383	5345-5353	VBN	denotes	repeated
T4384	5354-5361	VBG	denotes	washing
T4385	5362-5364	IN	denotes	of
T4386	5365-5368	DT	denotes	the
T4387	5369-5377	NN	denotes	membrane
T4388	5377-5378	,	denotes	,
T4389	5379-5382	DT	denotes	the
T4390	5383-5394	NNP	denotes	Supersignal
T4391	5395-5399	NNP	denotes	West
T4392	5400-5404	NNP	denotes	Pico
T4393	5405-5421	NNP	denotes	Chemiluminescent
T4394	5422-5425	NNP	denotes	Kit
T4395	5426-5429	VBD	denotes	was
T4396	5430-5437	VBN	denotes	applied
T4397	5438-5441	IN	denotes	for
T4398	5442-5450	NN	denotes	antibody
T4399	5451-5460	NN	denotes	detection
T4400	5461-5464	IN	denotes	per
T4401	5465-5468	DT	denotes	the
T4402	5469-5481	NN	denotes	manufacturer
T4403	5484-5496	NNS	denotes	instructions
T4404	5496-5497	.	denotes	.
T4405	5498-5505	JJ	denotes	Western
T4406	5506-5510	NN	denotes	blot
T4407	5511-5515	NNS	denotes	data
T4408	5516-5518	VBZ	denotes	is
T4409	5519-5528	VBN	denotes	presented
T4410	5529-5531	IN	denotes	as
T4411	5532-5533	DT	denotes	a
T4412	5534-5544	NN	denotes	percentage
T4413	5545-5547	IN	denotes	of
T4414	5548-5551	NNP	denotes	LPS
T4415	5552-5563	NN	denotes	stimulation
T4416	5563-5564	.	denotes	.
T4417	5565-5568	DT	denotes	The
T4418	5569-5579	NN	denotes	percentage
T4419	5580-5582	IN	denotes	of
T4420	5583-5586	NNP	denotes	LPS
T4421	5587-5598	NN	denotes	stimulation
T4422	5599-5602	VBD	denotes	was
T4423	5603-5613	VBN	denotes	calculated
T4424	5614-5616	IN	denotes	by
T4425	5617-5625	VBG	denotes	dividing
T4426	5626-5629	DT	denotes	the
T4427	5630-5634	JJ	denotes	mean
T4428	5635-5639	NN	denotes	band
T4429	5640-5645	NN	denotes	pixel
T4430	5646-5651	NN	denotes	total
T4431	5652-5655	IN	denotes	for
T4432	5656-5660	DT	denotes	each
T4433	5661-5670	NN	denotes	treatment
T4434	5671-5674	NN	denotes	arm
T4435	5675-5682	VBN	denotes	divided
T4436	5683-5685	IN	denotes	by
T4437	5686-5689	DT	denotes	the
T4438	5690-5694	JJ	denotes	mean
T4439	5695-5700	NN	denotes	pixel
T4440	5701-5706	NN	denotes	total
T4441	5707-5709	IN	denotes	of
T4442	5710-5717	NNS	denotes	samples
T4443	5718-5728	VBN	denotes	stimulated
T4444	5729-5733	IN	denotes	with
T4445	5734-5737	NNP	denotes	LPS
T4446	5738-5741	CC	denotes	and
T4447	5742-5753	VBG	denotes	multiplying
T4448	5754-5756	IN	denotes	by
T4449	5757-5760	CD	denotes	100
T4450	5760-5761	.	denotes	.
T4451	5762-5766	RB	denotes	Thus
T4452	5766-5767	,	denotes	,
T4453	5768-5771	NNP	denotes	LPS
T4454	5772-5783	NN	denotes	stimulation
T4455	5784-5786	VBZ	denotes	is
T4456	5787-5795	VBN	denotes	reported
T4457	5796-5798	IN	denotes	as
T4458	5799-5802	CD	denotes	100
T4459	5802-5803	NN	denotes	%
T4460	5804-5807	CC	denotes	and
T4461	5808-5812	DT	denotes	each
T4462	5813-5815	IN	denotes	of
T4463	5816-5819	DT	denotes	the
T4464	5820-5829	NN	denotes	treatment
T4465	5830-5834	NNS	denotes	arms
T4466	5835-5837	VBZ	denotes	is
T4467	5838-5846	VBN	denotes	reported
T4468	5847-5849	IN	denotes	as
T4469	5850-5851	DT	denotes	a
T4470	5852-5859	NN	denotes	percent
T4471	5860-5862	IN	denotes	of
T4472	5863-5866	NNP	denotes	LPS
T4473	5867-5878	NN	denotes	stimulation
T4474	5878-5879	.	denotes	.
T4907	5881-5886	NN	denotes	NF-κB
T4908	5887-5890	CC	denotes	and
T4909	5891-5895	NNP	denotes	CREB
T4910	5896-5911	NNP	denotes	Electrophoretic
T4911	5912-5920	NNP	denotes	Mobility
T4912	5921-5926	NNP	denotes	Shift
T4913	5927-5932	NNP	denotes	Assay
T4914	5933-5934	-LRB-	denotes	(
T4915	5934-5938	NNP	denotes	EMSA
T4916	5938-5939	-RRB-	denotes	)
T4917	5940-5943	DT	denotes	The
T4918	5944-5959	JJ	denotes	non-radioactive
T4919	5960-5970	NNP	denotes	LightShift
T4920	5971-5987	NNP	denotes	Chemiluminescent
T4921	5988-5992	NNP	denotes	EMSA
T4922	5993-5996	NN	denotes	Kit
T4923	5997-6000	VBD	denotes	was
T4924	6001-6005	VBN	denotes	used
T4925	6006-6008	TO	denotes	to
T4926	6009-6015	VB	denotes	detect
T4927	6016-6027	JJ	denotes	DNA-protein
T4928	6028-6040	NNS	denotes	interactions
T4929	6040-6041	.	denotes	.
T4930	6042-6045	DT	denotes	The
T4931	6046-6051	NN	denotes	NF-κB
T4932	6052-6053	CD	denotes	3
T4933	6053-6054	NN	denotes	′
T4934	6055-6061	NN	denotes	biotin
T4935	6062-6073	VBD	denotes	end-labeled
T4936	6074-6077	NNP	denotes	DNA
T4937	6078-6093	NN	denotes	oligonucleotide
T4938	6094-6098	VBD	denotes	used
T4939	6099-6101	IN	denotes	as
T4940	6102-6103	DT	denotes	a
T4941	6104-6109	NN	denotes	probe
T4942	6110-6113	IN	denotes	for
T4943	6114-6117	DT	denotes	the
T4944	6118-6122	NNP	denotes	EMSA
T4945	6123-6126	VBD	denotes	was
T4946	6127-6128	DT	denotes	a
T4947	6129-6134	JJ	denotes	42-bp
T4948	6135-6141	JJ	denotes	double
T4949	6142-6150	VBN	denotes	stranded
T4950	6151-6160	VBP	denotes	construct
T4951	6161-6162	-LRB-	denotes	(
T4952	6162-6163	CD	denotes	5
T4953	6163-6164	SYM	denotes	′
T4954	6164-6165	:	denotes	-
T4955	6165-6208	NN	denotes	TTGTTACAA-GGGGACTTTCCGCTGGGGACTTTCCAGGGAGGC
T4956	6211-6212	CD	denotes	3
T4957	6212-6213	NN	denotes	′
T4958	6213-6214	-RRB-	denotes	)
T4959	6215-6225	VBG	denotes	containing
T4960	6226-6229	CD	denotes	two
T4961	6230-6238	JJ	denotes	tandemly
T4962	6239-6247	VBN	denotes	repeated
T4963	6248-6253	NN	denotes	NF-κB
T4964	6254-6261	JJ	denotes	binding
T4965	6262-6267	NNS	denotes	sites
T4966	6268-6269	-LRB-	denotes	(
T4967	6269-6279	VBN	denotes	underlined
T4968	6279-6280	-RRB-	denotes	)
T4969	6280-6281	.	denotes	.
T4970	6282-6293	NN	denotes	Specificity
T4971	6294-6297	VBD	denotes	was
T4972	6298-6308	VBN	denotes	determined
T4973	6309-6311	IN	denotes	by
T4974	6312-6313	DT	denotes	a
T4975	6314-6325	NN	denotes	competition
T4976	6326-6331	NN	denotes	assay
T4977	6332-6336	IN	denotes	with
T4978	6337-6340	DT	denotes	the
T4979	6341-6349	NN	denotes	addition
T4980	6350-6352	IN	denotes	of
T4981	6353-6356	CD	denotes	200
T4982	6357-6362	JJ	denotes	molar
T4983	6363-6369	NN	denotes	excess
T4984	6370-6372	IN	denotes	of
T4985	6373-6382	JJ	denotes	unlabeled
T4986	6383-6389	JJ	denotes	double
T4987	6390-6398	VBN	denotes	stranded
T4988	6399-6404	NN	denotes	NF-κB
T4989	6405-6420	NN	denotes	oligonucleotide
T4990	6420-6421	.	denotes	.
T4991	6422-6425	DT	denotes	The
T4992	6426-6430	NN	denotes	CREB
T4993	6431-6432	CD	denotes	3
T4994	6432-6433	NN	denotes	′
T4995	6434-6440	NN	denotes	biotin
T4996	6441-6452	VBD	denotes	end-labeled
T4997	6453-6456	NNP	denotes	DNA
T4998	6457-6472	NN	denotes	oligonucleotide
T4999	6473-6477	VBD	denotes	used
T5000	6478-6480	IN	denotes	as
T5001	6481-6482	DT	denotes	a
T5002	6483-6488	NN	denotes	probe
T5003	6489-6492	IN	denotes	for
T5004	6493-6496	DT	denotes	the
T5005	6497-6501	NNP	denotes	EMSA
T5006	6502-6505	VBD	denotes	was
T5007	6506-6507	DT	denotes	a
T5008	6508-6513	JJ	denotes	23-bp
T5009	6514-6520	JJ	denotes	double
T5010	6521-6529	VBN	denotes	stranded
T5011	6530-6539	VBP	denotes	construct
T5012	6540-6541	-LRB-	denotes	(
T5013	6541-6542	CD	denotes	5
T5014	6542-6543	SYM	denotes	′
T5015	6543-6544	:	denotes	-
T5016	6544-6547	NNP	denotes	TTT
T5017	6548-6551	NNP	denotes	TCG
T5018	6552-6555	NNP	denotes	AGC
T5019	6556-6571	NNP	denotes	TCTGACGTCAGA-GC
T5020	6574-6575	CD	denotes	3
T5021	6575-6576	NN	denotes	′
T5022	6576-6577	-RRB-	denotes	)
T5023	6578-6588	VBG	denotes	containing
T5024	6589-6592	DT	denotes	the
T5025	6593-6596	NNP	denotes	CRE
T5026	6597-6606	NN	denotes	consensus
T5027	6607-6615	NN	denotes	sequence
T5028	6616-6617	-LRB-	denotes	(
T5029	6617-6627	VBN	denotes	underlined
T5030	6627-6628	-RRB-	denotes	)
T5031	6628-6629	.	denotes	.
T5032	6630-6641	NN	denotes	Specificity
T5033	6642-6645	VBD	denotes	was
T5034	6646-6656	VBN	denotes	determined
T5035	6657-6659	IN	denotes	by
T5036	6660-6661	DT	denotes	a
T5037	6662-6673	NN	denotes	competition
T5038	6674-6679	NN	denotes	assay
T5039	6680-6684	IN	denotes	with
T5040	6685-6688	DT	denotes	the
T5041	6689-6697	NN	denotes	addition
T5042	6698-6700	IN	denotes	of
T5043	6701-6704	CD	denotes	200
T5044	6705-6710	JJ	denotes	molar
T5045	6711-6717	NN	denotes	excess
T5046	6718-6720	IN	denotes	of
T5047	6721-6730	JJ	denotes	unlabeled
T5048	6731-6737	JJ	denotes	double
T5049	6738-6746	VBN	denotes	stranded
T5050	6747-6751	NNP	denotes	CREB
T5051	6752-6767	NN	denotes	oligonucleotide
T5052	6767-6768	.	denotes	.
T5053	6769-6776	NNP	denotes	Nuclear
T5054	6777-6785	NNS	denotes	extracts
T5055	6786-6787	-LRB-	denotes	(
T5056	6787-6789	CD	denotes	10
T5057	6790-6792	NN	denotes	μg
T5058	6792-6793	-RRB-	denotes	)
T5059	6794-6798	VBD	denotes	were
T5060	6799-6808	VBN	denotes	incubated
T5061	6809-6813	IN	denotes	with
T5062	6814-6815	CD	denotes	5
T5063	6816-6818	NNP	denotes	nM
T5064	6819-6824	NNP	denotes	NF-κB
T5065	6825-6827	CC	denotes	or
T5066	6828-6832	NNP	denotes	CREB
T5067	6833-6838	NN	denotes	probe
T5068	6839-6840	-LRB-	denotes	(
T5069	6840-6845	NN	denotes	NF-kB
T5070	6845-6846	:	denotes	:
T5071	6847-6849	CD	denotes	1x
T5072	6850-6857	JJ	denotes	binding
T5073	6858-6864	NN	denotes	buffer
T5074	6864-6865	,	denotes	,
T5075	6866-6868	CD	denotes	50
T5076	6869-6871	NNP	denotes	mM
T5077	6872-6875	NNP	denotes	KCl
T5078	6875-6876	,	denotes	,
T5079	6877-6878	CD	denotes	1
T5080	6879-6881	NNP	denotes	mM
T5081	6882-6886	NNP	denotes	EDTA
T5082	6886-6887	,	denotes	,
T5083	6888-6889	CD	denotes	1
T5084	6890-6892	NNP	denotes	mM
T5085	6893-6896	NNP	denotes	DTT
T5086	6896-6897	,	denotes	,
T5087	6898-6901	CD	denotes	0.1
T5088	6901-6902	NN	denotes	%
T5089	6903-6907	CD	denotes	NP40
T5090	6907-6908	,	denotes	,
T5091	6909-6911	CD	denotes	10
T5092	6911-6912	NN	denotes	%
T5093	6913-6921	NN	denotes	glycerol
T5094	6921-6922	,	denotes	,
T5095	6923-6926	CC	denotes	and
T5096	6927-6929	CD	denotes	50
T5097	6930-6932	NN	denotes	ng
T5098	6932-6933	NN	denotes	/
T5099	6933-6935	NN	denotes	μl
T5100	6936-6940	NN	denotes	poly
T5101	6941-6946	NN	denotes	dI-dC
T5102	6946-6947	,	denotes	,
T5103	6948-6952	NNP	denotes	CREB
T5104	6952-6953	:	denotes	:
T5105	6954-6956	JJ	denotes	1x
T5106	6957-6964	JJ	denotes	binding
T5107	6965-6971	NN	denotes	buffer
T5108	6971-6972	,	denotes	,
T5109	6973-6975	CD	denotes	20
T5110	6976-6978	NNP	denotes	mM
T5111	6979-6983	NNP	denotes	Tris
T5112	6983-6984	,	denotes	,
T5113	6985-6987	NNP	denotes	pH
T5114	6988-6991	CD	denotes	7.5
T5115	6991-6992	,	denotes	,
T5116	6993-6995	CD	denotes	50
T5117	6996-6998	NNP	denotes	mM
T5118	6999-7002	NNP	denotes	KCl
T5119	7002-7003	,	denotes	,
T5120	7004-7005	CD	denotes	1
T5121	7006-7008	NNP	denotes	mM
T5122	7009-7013	NNP	denotes	EDTA
T5123	7013-7014	,	denotes	,
T5124	7015-7016	CD	denotes	1
T5125	7017-7019	NNP	denotes	mM
T5126	7020-7023	NNP	denotes	DTT
T5127	7023-7024	,	denotes	,
T5128	7025-7029	CD	denotes	0.10
T5129	7029-7030	NN	denotes	%
T5130	7031-7035	CD	denotes	NP40
T5131	7035-7036	,	denotes	,
T5132	7037-7038	CD	denotes	6
T5133	7038-7039	NN	denotes	%
T5134	7040-7048	NN	denotes	glycerol
T5135	7048-7049	,	denotes	,
T5136	7050-7053	CD	denotes	0.1
T5137	7054-7059	NNP	denotes	mg/mL
T5138	7060-7063	NNP	denotes	BSA
T5139	7064-7067	CC	denotes	and
T5140	7068-7070	CD	denotes	50
T5141	7071-7073	NN	denotes	ng
T5142	7073-7074	NN	denotes	/
T5143	7074-7076	NN	denotes	μl
T5144	7077-7081	NN	denotes	poly
T5145	7082-7087	NN	denotes	dI-dC
T5146	7087-7088	-RRB-	denotes	)
T5147	7089-7092	CC	denotes	and
T5148	7093-7097	VBD	denotes	were
T5149	7098-7102	RB	denotes	then
T5150	7103-7118	VBN	denotes	electrophoresed
T5151	7119-7126	IN	denotes	through
T5152	7127-7128	DT	denotes	a
T5153	7129-7130	CD	denotes	6
T5154	7130-7131	NN	denotes	%
T5155	7132-7135	NN	denotes	DNA
T5156	7136-7147	NN	denotes	retardation
T5157	7148-7151	NN	denotes	gel
T5158	7152-7154	IN	denotes	at
T5159	7155-7159	CD	denotes	100V
T5160	7160-7163	IN	denotes	for
T5161	7164-7166	CD	denotes	90
T5162	7167-7174	NNS	denotes	minutes
T5163	7174-7175	.	denotes	.
T5164	7176-7179	DT	denotes	The
T5165	7180-7184	NNS	denotes	gels
T5166	7185-7189	VBD	denotes	were
T5167	7190-7209	RB	denotes	electrophoretically
T5168	7210-7221	VBN	denotes	transferred
T5169	7222-7224	IN	denotes	at
T5170	7225-7230	NNP	denotes	380mA
T5171	7231-7234	IN	denotes	for
T5172	7235-7236	CD	denotes	1
T5173	7237-7241	NN	denotes	hour
T5174	7242-7244	IN	denotes	on
T5175	7245-7248	NN	denotes	ice
T5176	7249-7251	TO	denotes	to
T5177	7252-7253	DT	denotes	a
T5178	7254-7264	RB	denotes	positively
T5179	7265-7272	VBN	denotes	charged
T5180	7273-7278	NN	denotes	nylon
T5181	7279-7287	NN	denotes	membrane
T5182	7288-7291	CC	denotes	and
T5183	7292-7303	RB	denotes	immediately
T5184	7304-7316	JJ	denotes	cross-linked
T5185	7317-7320	IN	denotes	for
T5186	7321-7323	CD	denotes	15
T5187	7324-7331	NNS	denotes	minutes
T5188	7332-7336	IN	denotes	with
T5189	7337-7338	DT	denotes	a
T5190	7339-7341	NNP	denotes	UV
T5191	7342-7358	NN	denotes	transilluminator
T5192	7359-7367	VBN	denotes	equipped
T5193	7368-7372	IN	denotes	with
T5194	7373-7374	DT	denotes	a
T5195	7375-7378	CD	denotes	312
T5196	7379-7381	NN	denotes	nm
T5197	7382-7386	NN	denotes	bulb
T5198	7386-7387	.	denotes	.
T5199	7388-7412	JJ	denotes	Streptavidin-horseradish
T5200	7413-7423	NN	denotes	peroxidase
T5201	7424-7433	NN	denotes	conjugate
T5202	7434-7437	CC	denotes	and
T5203	7438-7441	DT	denotes	the
T5204	7442-7452	NNP	denotes	LightShift
T5205	7453-7469	NNP	denotes	Chemiluminescent
T5206	7470-7479	NNP	denotes	Substrate
T5207	7480-7484	VBD	denotes	were
T5208	7485-7489	VBN	denotes	used
T5209	7490-7492	TO	denotes	to
T5210	7493-7499	VB	denotes	detect
T5211	7500-7503	DT	denotes	the
T5212	7504-7510	NN	denotes	biotin
T5213	7511-7522	VBD	denotes	end-labeled
T5214	7523-7526	NNP	denotes	DNA
T5215	7526-7527	.	denotes	.
T5216	7528-7531	DT	denotes	The
T5217	7532-7537	NN	denotes	nylon
T5218	7538-7547	NNS	denotes	membranes
T5219	7548-7552	VBD	denotes	were
T5220	7553-7560	VBN	denotes	exposed
T5221	7561-7563	TO	denotes	to
T5222	7564-7569	VB	denotes	x-ray
T5223	7570-7574	NN	denotes	film
T5224	7575-7578	IN	denotes	for
T5225	7579-7580	CD	denotes	1
T5226	7581-7582	CD	denotes	3
T5227	7583-7590	NNS	denotes	minutes
T5228	7591-7594	IN	denotes	for
T5229	7595-7604	NN	denotes	detection
T5230	7604-7605	.	denotes	.
T5650	7607-7611	NN	denotes	Role
T5651	7612-7614	IN	denotes	of
T5652	7615-7618	NNP	denotes	PKA
T5653	7619-7621	IN	denotes	on
T5654	7622-7633	JJ	denotes	LPS-induced
T5655	7634-7645	NN	denotes	mononuclear
T5656	7646-7650	NN	denotes	cell
T5657	7651-7656	JJ	denotes	TNF-α
T5658	7657-7667	NN	denotes	production
T5659	7668-7670	TO	denotes	To
T5660	7671-7679	VB	denotes	evaluate
T5661	7680-7683	DT	denotes	the
T5662	7684-7688	NN	denotes	role
T5663	7689-7691	IN	denotes	of
T5664	7692-7695	NNP	denotes	PKA
T5665	7696-7698	IN	denotes	on
T5666	7699-7704	JJ	denotes	TNF-α
T5667	7705-7715	NN	denotes	production
T5668	7716-7718	IN	denotes	in
T5669	7719-7730	NN	denotes	mononuclear
T5670	7731-7736	NNS	denotes	cells
T5671	7737-7740	CC	denotes	and
T5672	7741-7744	PRP$	denotes	its
T5673	7745-7756	NN	denotes	involvement
T5674	7757-7759	IN	denotes	in
T5675	7760-7763	DT	denotes	the
T5676	7764-7775	NN	denotes	attenuation
T5677	7776-7778	IN	denotes	of
T5678	7779-7790	JJ	denotes	LPS-induced
T5679	7791-7796	JJ	denotes	TNF-α
T5680	7797-7807	NN	denotes	production
T5681	7808-7816	VBD	denotes	observed
T5682	7817-7826	VBG	denotes	following
T5683	7827-7830	NNP	denotes	PTX
T5684	7831-7840	NN	denotes	treatment
T5685	7840-7841	,	denotes	,
T5686	7842-7845	NNP	denotes	H89
T5687	7845-7846	,	denotes	,
T5688	7847-7848	DT	denotes	a
T5689	7849-7857	JJ	denotes	specific
T5690	7858-7861	NNP	denotes	PKA
T5691	7862-7871	NN	denotes	inhibitor
T5692	7871-7872	,	denotes	,
T5693	7873-7876	VBD	denotes	was
T5694	7877-7885	VBN	denotes	utilized
T5695	7885-7886	.	denotes	.
T5696	7887-7895	VBN	denotes	Isolated
T5697	7896-7907	NN	denotes	mononuclear
T5698	7908-7913	NNS	denotes	cells
T5699	7914-7918	VBD	denotes	were
T5700	7919-7928	VBN	denotes	incubated
T5701	7929-7938	VBG	denotes	according
T5702	7939-7941	TO	denotes	to
T5703	7942-7945	DT	denotes	the
T5704	7946-7955	NN	denotes	treatment
T5705	7956-7962	NNS	denotes	groups
T5706	7963-7972	VBN	denotes	described
T5707	7973-7978	IN	denotes	above
T5708	7979-7981	IN	denotes	in
T5709	7982-7985	DT	denotes	the
T5710	7986-7994	NN	denotes	presence
T5711	7995-7998	CC	denotes	and
T5712	7999-8006	NN	denotes	absence
T5713	8007-8009	IN	denotes	of
T5714	8010-8022	NN	denotes	pretreatment
T5715	8023-8027	IN	denotes	with
T5716	8028-8031	NNP	denotes	H89
T5717	8032-8033	-LRB-	denotes	(
T5718	8033-8035	CD	denotes	10
T5719	8036-8038	NNP	denotes	μM
T5720	8038-8039	-RRB-	denotes	)
T5721	8040-8043	IN	denotes	for
T5722	8044-8045	CD	denotes	1
T5723	8046-8050	NN	denotes	hour
T5724	8051-8053	IN	denotes	at
T5725	8054-8058	NNP	denotes	37ºC
T5726	8058-8059	.	denotes	.
T5727	8060-8063	DT	denotes	The
T5728	8064-8068	NN	denotes	dose
T5729	8069-8071	IN	denotes	of
T5730	8072-8075	NNP	denotes	H89
T5731	8076-8079	VBD	denotes	was
T5732	8080-8086	VBN	denotes	chosen
T5733	8087-8092	VBN	denotes	based
T5734	8093-8095	IN	denotes	on
T5735	8096-8104	JJ	denotes	previous
T5736	8105-8109	NN	denotes	work
T5737	8110-8115	WDT	denotes	which
T5738	8116-8128	VBD	denotes	demonstrated
T5739	8129-8137	JJ	denotes	specific
T5740	8138-8141	CC	denotes	and
T5741	8142-8150	JJ	denotes	complete
T5742	8151-8161	NN	denotes	inhibition
T5743	8162-8164	IN	denotes	of
T5744	8165-8168	NNP	denotes	PKA
T5745	8169-8171	IN	denotes	at
T5746	8172-8176	DT	denotes	this
T5747	8177-8190	NN	denotes	concentration
T5748	8190-8193	CD	denotes	.10
T5749	8194-8199	IN	denotes	Since
T5750	8200-8203	DT	denotes	the
T5751	8204-8212	NN	denotes	activity
T5752	8213-8215	IN	denotes	of
T5753	8216-8220	DT	denotes	this
T5754	8221-8230	NN	denotes	inhibitor
T5755	8231-8233	VBZ	denotes	is
T5756	8234-8238	DT	denotes	both
T5757	8239-8247	JJ	denotes	specific
T5758	8248-8251	CC	denotes	and
T5759	8252-8262	JJ	denotes	consistent
T5760	8263-8265	IN	denotes	at
T5761	8266-8270	DT	denotes	this
T5762	8271-8280	VBN	denotes	specified
T5763	8281-8285	NN	denotes	dose
T5764	8285-8286	,	denotes	,
T5765	8287-8289	PRP	denotes	we
T5766	8290-8293	VBD	denotes	did
T5767	8294-8297	RB	denotes	not
T5768	8298-8305	VB	denotes	include
T5769	8306-8317	NNS	denotes	experiments
T5770	8318-8322	IN	denotes	with
T5771	8323-8333	JJ	denotes	additional
T5772	8334-8337	NNP	denotes	PKA
T5773	8338-8348	NNS	denotes	inhibitors
T5774	8349-8353	IN	denotes	with
T5775	8354-8358	DT	denotes	this
T5776	8359-8362	NN	denotes	set
T5777	8363-8365	IN	denotes	of
T5778	8366-8377	NNS	denotes	experiments
T5779	8377-8378	.	denotes	.
T5780	8379-8382	DT	denotes	The
T5781	8383-8388	JJ	denotes	TNF-α
T5782	8389-8402	NN	denotes	concentration
T5783	8403-8405	IN	denotes	in
T5784	8406-8409	DT	denotes	the
T5785	8410-8421	NN	denotes	supernatant
T5786	8422-8425	VBD	denotes	was
T5787	8426-8434	VBN	denotes	measured
T5788	8435-8449	RB	denotes	quantitatively
T5789	8450-8452	IN	denotes	by
T5790	8453-8458	NNP	denotes	ELISA
T5791	8458-8459	.	denotes	.
T5792	8460-8463	DT	denotes	The
T5793	8464-8471	NNS	denotes	results
T5794	8472-8475	VBP	denotes	are
T5795	8476-8485	VBN	denotes	expressed
T5796	8486-8488	IN	denotes	in
T5797	8489-8494	NNP	denotes	pg/mL
T5798	8494-8495	.	denotes	.
T6072	8497-8508	NNP	denotes	Statistical
T6073	8509-8517	NNP	denotes	Analysis
T6074	8518-8521	NNP	denotes	The
T6075	8522-8534	JJ	denotes	experimental
T6076	8535-8542	NNS	denotes	results
T6077	8543-8551	VBN	denotes	obtained
T6078	8552-8554	IN	denotes	in
T6079	8555-8559	DT	denotes	this
T6080	8560-8565	NN	denotes	study
T6081	8566-8570	VBD	denotes	were
T6082	8571-8578	VBN	denotes	derived
T6083	8579-8583	IN	denotes	from
T6084	8584-8588	CD	denotes	four
T6085	8589-8597	JJ	denotes	separate
T6086	8598-8609	NNS	denotes	experiments
T6087	8610-8614	IN	denotes	with
T6088	8615-8622	JJ	denotes	healthy
T6089	8623-8632	NN	denotes	volunteer
T6090	8633-8639	NNS	denotes	donors
T6091	8639-8640	.	denotes	.
T6092	8641-8645	DT	denotes	Each
T6093	8646-8651	NN	denotes	assay
T6094	8652-8655	VBD	denotes	was
T6095	8656-8665	VBN	denotes	performed
T6096	8666-8668	IN	denotes	in
T6097	8669-8678	VBP	denotes	duplicate
T6098	8679-8681	CC	denotes	or
T6099	8682-8692	VBP	denotes	triplicate
T6100	8693-8698	WRB	denotes	where
T6101	8699-8710	JJ	denotes	appropriate
T6102	8710-8711	.	denotes	.
T6103	8712-8716	NNP	denotes	Data
T6104	8717-8719	VBZ	denotes	is
T6105	8720-8729	VBN	denotes	presented
T6106	8730-8732	IN	denotes	as
T6107	8733-8736	DT	denotes	the
T6108	8737-8741	JJ	denotes	mean
T6109	8742-8743	NN	denotes	±
T6110	8744-8747	NNP	denotes	SEM
T6111	8747-8748	.	denotes	.
T6112	8749-8760	NNP	denotes	Statistical
T6113	8761-8772	NNS	denotes	differences
T6114	8773-8780	IN	denotes	between
T6115	8781-8787	NNS	denotes	groups
T6116	8788-8792	VBD	denotes	were
T6117	8793-8803	VBN	denotes	determined
T6118	8804-8806	IN	denotes	by
T6119	8807-8814	JJ	denotes	one-way
T6120	8815-8823	NN	denotes	analysis
T6121	8824-8826	IN	denotes	of
T6122	8827-8835	NN	denotes	variance
T6123	8836-8837	-LRB-	denotes	(
T6124	8837-8842	NNP	denotes	ANOVA
T6125	8842-8843	-RRB-	denotes	)
T6126	8844-8848	IN	denotes	with
T6127	8849-8850	DT	denotes	a
T6128	8851-8861	NNP	denotes	Bonferroni
T6129	8862-8872	NN	denotes	correction
T6130	8872-8873	.	denotes	.
T6131	8874-8885	NNP	denotes	Statistical
T6132	8886-8898	NN	denotes	significance
T6133	8899-8902	VBD	denotes	was
T6134	8903-8910	VBN	denotes	defined
T6135	8911-8913	IN	denotes	as
T6136	8914-8915	NNP	denotes	P
T6137	8916-8917	CD	denotes	<
T6138	8918-8922	CD	denotes	0.05
T6139	8922-8923	.	denotes	.
R1894	T2467	T2467	ROOT	approved,approved
R1895	T2468	T2467	agent	by,approved
R1896	T2469	T2473	det	the,Program
R1897	T2470	T2472	compound	Human,Protections
R1898	T2471	T2472	compound	Research,Protections
R1899	T2472	T2473	compound	Protections,Program
R1900	T2473	T2468	pobj	Program,by
R1901	T2474	T2473	cc	and,Program
R1902	T2475	T2478	det	the,Board
R1903	T2476	T2478	compound	Institutional,Board
R1904	T2477	T2478	compound	Review,Board
R1905	T2478	T2473	conj	Board,Program
R1906	T2479	T2467	punct	.,approved
R1907	T2480	T2481	amod	Written,consent
R1908	T2481	T2488	nsubjpass	consent,obtained
R1909	T2482	T2483	aux	to,participate
R1910	T2483	T2481	relcl	participate,consent
R1911	T2484	T2483	prep	in,participate
R1912	T2485	T2486	det	the,study
R1913	T2486	T2484	pobj	study,in
R1914	T2487	T2488	auxpass	was,obtained
R1915	T2488	T2488	ROOT	obtained,obtained
R1916	T2489	T2488	prep	from,obtained
R1917	T2490	T2491	det	all,volunteers
R1918	T2491	T2489	pobj	volunteers,from
R1919	T2492	T2488	advmod	prior,obtained
R1920	T2493	T2492	prep	to,prior
R1921	T2494	T2493	pobj	blood,to
R1922	T2495	T2496	amod	"donation. Heparinized",Vacutainers
R1923	T2496	T2493	pobj	Vacutainers,to
R1924	T2497	T2498	auxpass	were,purchased
R1925	T2498	T2488	conj	purchased,obtained
R1926	T2499	T2498	prep	from,purchased
R1927	T2500	T2501	compound	Becton,Dickinson
R1928	T2501	T2499	pobj	Dickinson,from
R1929	T2502	T2501	punct	(,Dickinson
R1930	T2503	T2504	compound	San,Jose
R1931	T2504	T2501	appos	Jose,Dickinson
R1932	T2505	T2504	punct	",",Jose
R1933	T2506	T2501	conj	CA,Dickinson
R1934	T2507	T2488	punct	),obtained
R1935	T2508	T2488	punct	.,obtained
R1936	T2509	T2512	nummod	1.5-mL,tubes
R1937	T2510	T2512	compound	Eppendorf,tubes
R1938	T2511	T2512	compound	centrifuge,tubes
R1939	T2512	T2514	nsubjpass	tubes,purchased
R1940	T2513	T2514	auxpass	were,purchased
R1941	T2514	T2514	ROOT	purchased,purchased
R1942	T2515	T2514	prep	from,purchased
R1943	T2516	T2517	compound	Fisher,Scientific
R1944	T2517	T2515	pobj	Scientific,from
R1945	T2518	T2517	punct	(,Scientific
R1946	T2519	T2517	appos	Pittsburg,Scientific
R1947	T2520	T2519	punct	",",Pittsburg
R1948	T2521	T2517	conj	PA,Scientific
R1949	T2522	T2514	punct	),purchased
R1950	T2523	T2514	punct	.,purchased
R1951	T2524	T2527	poss	Hank,solution
R1952	T2525	T2527	amod	balanced,solution
R1953	T2526	T2527	compound	salt,solution
R1954	T2527	T2535	nsubjpass	solution,obtained
R1955	T2528	T2529	punct	(,HBSS
R1956	T2529	T2527	appos	HBSS,solution
R1957	T2530	T2529	punct	),HBSS
R1958	T2531	T2527	cc	and,solution
R1959	T2532	T2533	compound	RPMI,1640
R1960	T2533	T2535	nsubjpass	1640,obtained
R1961	T2534	T2535	auxpass	were,obtained
R1962	T2535	T2535	ROOT	obtained,obtained
R1963	T2536	T2535	prep	from,obtained
R1964	T2537	T2538	compound	Irvine,Scientific
R1965	T2538	T2536	pobj	Scientific,from
R1966	T2539	T2538	punct	(,Scientific
R1967	T2540	T2541	compound	Santa,Ana
R1968	T2541	T2538	appos	Ana,Scientific
R1969	T2542	T2541	punct	",",Ana
R1970	T2543	T2538	conj	CA,Scientific
R1971	T2544	T2535	punct	),obtained
R1972	T2545	T2535	punct	.,obtained
R1973	T2546	T2548	compound	LPS,coli serotype
R1974	T2547	T2548	compound	from Escherichia,coli serotype
R1975	T2548	T2555	nsubjpass	coli serotype,purchased
R1976	T2549	T2548	nummod	0111,coli serotype
R1977	T2550	T2548	punct	:,coli serotype
R1978	T2551	T2548	appos	B4,coli serotype
R1979	T2552	T2551	cc	and,B4
R1980	T2553	T2551	conj	PTX,B4
R1981	T2554	T2555	auxpass	were,purchased
R1982	T2555	T2555	ROOT	purchased,purchased
R1983	T2556	T2555	prep	from,purchased
R1984	T2557	T2556	pobj	Sigma,from
R1985	T2558	T2557	punct	(,Sigma
R1986	T2559	T2560	compound	St.,Louis
R1987	T2560	T2557	appos	Louis,Sigma
R1988	T2561	T2557	punct	",",Sigma
R1989	T2562	T2557	appos	MO,Sigma
R1990	T2563	T2557	punct	),Sigma
R1991	T2564	T2555	punct	.,purchased
R1992	T2565	T2567	det	The,inhibitor
R1993	T2566	T2567	compound	PKA,inhibitor
R1994	T2567	T2591	nsubjpass	inhibitor,purchased
R1995	T2568	T2567	punct	",",inhibitor
R1996	T2569	T2571	compound	N,[
R1997	T2570	T2571	punct	-,[
R1998	T2571	T2581	compound	[,]
R1999	T2572	T2576	meta	2,p-bromocinnamyl
R2000	T2573	T2576	punct	-,p-bromocinnamyl
R2001	T2574	T2576	punct	(,p-bromocinnamyl
R2002	T2575	T2576	punct	(,p-bromocinnamyl
R2003	T2576	T2581	nmod	p-bromocinnamyl,]
R2004	T2577	T2576	punct	),p-bromocinnamyl
R2005	T2578	T2576	amod	amino,p-bromocinnamyl
R2006	T2579	T2576	punct	),p-bromocinnamyl
R2007	T2580	T2581	compound	ethyl,]
R2008	T2581	T2567	appos	],inhibitor
R2009	T2582	T2584	punct	-5,isoguinolinesulfonamide-2HCl
R2010	T2583	T2584	punct	-,isoguinolinesulfonamide-2HCl
R2011	T2584	T2585	compound	isoguinolinesulfonamide-2HCl,]
R2012	T2585	T2581	appos	],]
R2013	T2586	T2587	punct	(,H89
R2014	T2587	T2585	appos	H89,]
R2015	T2588	T2585	punct	),]
R2016	T2589	T2591	punct	",",purchased
R2017	T2590	T2591	auxpass	was,purchased
R2018	T2591	T2591	ROOT	purchased,purchased
R2019	T2592	T2591	prep	from,purchased
R2020	T2593	T2592	pobj	Calbiochem,from
R2021	T2594	T2593	punct	(,Calbiochem
R2022	T2595	T2596	compound	La,Jolla
R2023	T2596	T2593	appos	Jolla,Calbiochem
R2024	T2597	T2596	punct	",",Jolla
R2025	T2598	T2596	appos	CA,Jolla
R2026	T2599	T2593	punct	),Calbiochem
R2027	T2600	T2591	punct	.,purchased
R2028	T2601	T2632	nsubjpass	Antibodies,purchased
R2029	T2602	T2601	prep	for,Antibodies
R2030	T2603	T2604	compound	phosphorylated,CREB
R2031	T2604	T2602	pobj	CREB,for
R2032	T2605	T2606	punct	(,serine
R2033	T2606	T2604	appos	serine,CREB
R2034	T2607	T2606	nummod	133,serine
R2035	T2608	T2606	punct	),serine
R2036	T2609	T2604	punct	",",CREB
R2037	T2610	T2611	compound	phosphorylated,I-κBα
R2038	T2611	T2604	conj	I-κBα,CREB
R2039	T2612	T2613	punct	(,serines
R2040	T2613	T2611	parataxis	serines,I-κBα
R2041	T2614	T2613	nummod	32,serines
R2042	T2615	T2614	cc	and,32
R2043	T2616	T2614	conj	36,32
R2044	T2617	T2613	punct	),serines
R2045	T2618	T2632	punct	",",purchased
R2046	T2619	T2620	compound	phosphorylated,NF-κB
R2047	T2620	T2632	nsubjpass	NF-κB,purchased
R2048	T2621	T2622	nummod	p65,subunit
R2049	T2622	T2620	appos	subunit,NF-κB
R2050	T2623	T2624	punct	(,serine
R2051	T2624	T2622	appos	serine,subunit
R2052	T2625	T2624	nummod	276,serine
R2053	T2626	T2624	punct	),serine
R2054	T2627	T2624	punct	",",serine
R2055	T2628	T2624	cc	and,serine
R2056	T2629	T2630	amod	secondary,antibodies
R2057	T2630	T2624	conj	antibodies,serine
R2058	T2631	T2632	auxpass	were,purchased
R2059	T2632	T2632	ROOT	purchased,purchased
R2060	T2633	T2632	prep	from,purchased
R2061	T2634	T2635	compound	Cell,Signaling
R2062	T2635	T2633	pobj	Signaling,from
R2063	T2636	T2637	punct	(,Beverly
R2064	T2637	T2635	appos	Beverly,Signaling
R2065	T2638	T2637	punct	",",Beverly
R2066	T2639	T2637	appos	MA,Beverly
R2067	T2640	T2637	punct	),Beverly
R2068	T2641	T2632	punct	.,purchased
R2069	T2642	T2642	ROOT	Dextran,Dextran
R2070	T2643	T2642	nummod	T500,Dextran
R2071	T2644	T2647	cc	and,received
R2072	T2645	T2647	nsubjpass	Percoll,received
R2073	T2646	T2647	auxpass	were,received
R2074	T2647	T2647	ROOT	received,received
R2075	T2648	T2647	prep	from,received
R2076	T2649	T2651	compound	GE,Biosciences
R2077	T2650	T2651	compound	Healthcare/Amersham,Biosciences
R2078	T2651	T2648	pobj	Biosciences,from
R2079	T2652	T2651	punct	(,Biosciences
R2080	T2653	T2651	npadvmod	Piscataway,Biosciences
R2081	T2654	T2653	punct	",",Piscataway
R2082	T2655	T2653	appos	NJ,Piscataway
R2083	T2656	T2653	punct	),Piscataway
R2084	T2657	T2647	punct	.,received
R2085	T2658	T2661	det	The,assay
R2086	T2659	T2661	nmod	enzyme-linked,assay
R2087	T2660	T2661	compound	immunosorbent,assay
R2088	T2661	T2668	nsubjpass	assay,obtained
R2089	T2662	T2661	punct	(,assay
R2090	T2663	T2661	appos	ELISA,assay
R2091	T2664	T2661	punct	),assay
R2092	T2665	T2668	prep	for,obtained
R2093	T2666	T2665	pobj	TNF-α,for
R2094	T2667	T2668	auxpass	was,obtained
R2095	T2668	T2668	ROOT	obtained,obtained
R2096	T2669	T2668	prep	from,obtained
R2097	T2670	T2672	compound	Quantikine,Systems
R2098	T2671	T2672	compound	R&D,Systems
R2099	T2672	T2669	pobj	Systems,from
R2100	T2673	T2672	punct	(,Systems
R2101	T2674	T2672	npadvmod	Minneapolis,Systems
R2102	T2675	T2674	punct	",",Minneapolis
R2103	T2676	T2674	appos	MN,Minneapolis
R2104	T2677	T2674	punct	),Minneapolis
R2105	T2678	T2668	punct	.,obtained
R2106	T2679	T2683	nummod	Tris-glycine,gels
R2107	T2680	T2679	cc	and,Tris-glycine
R2108	T2681	T2679	conj	DNA,Tris-glycine
R2109	T2682	T2683	compound	retardation,gels
R2110	T2683	T2694	nsubjpass	gels,obtained
R2111	T2684	T2686	punct	",",membranes
R2112	T2685	T2686	amod	nitrocellulose,membranes
R2113	T2686	T2683	conj	membranes,gels
R2114	T2687	T2686	punct	",",membranes
R2115	T2688	T2686	cc	and,membranes
R2116	T2689	T2690	amod	western,blot
R2117	T2690	T2692	compound	blot,buffers
R2118	T2691	T2692	compound	running,buffers
R2119	T2692	T2686	conj	buffers,membranes
R2120	T2693	T2694	auxpass	were,obtained
R2121	T2694	T2694	ROOT	obtained,obtained
R2122	T2695	T2694	prep	from,obtained
R2123	T2696	T2695	pobj	Invitrogen,from
R2124	T2697	T2696	punct	(,Invitrogen
R2125	T2698	T2696	appos	Carlsbad,Invitrogen
R2126	T2699	T2698	punct	",",Carlsbad
R2127	T2700	T2698	conj	CA,Carlsbad
R2128	T2701	T2696	punct	),Invitrogen
R2129	T2702	T2694	punct	.,obtained
R2130	T2703	T2704	compound	NE-PER,®
R2131	T2704	T2709	nmod	®,reagents
R2132	T2705	T2709	amod	nuclear,reagents
R2133	T2706	T2705	cc	and,nuclear
R2134	T2707	T2705	conj	cytoplasmic,nuclear
R2135	T2708	T2709	compound	extraction,reagents
R2136	T2709	T2731	nsubjpass	reagents,purchased
R2137	T2710	T2715	punct	",",kit
R2138	T2711	T2713	det	the,protein
R2139	T2712	T2713	compound	BCA,protein
R2140	T2713	T2715	compound	protein,kit
R2141	T2714	T2715	compound	reagent,kit
R2142	T2715	T2731	nsubjpass	kit,purchased
R2143	T2716	T2715	punct	",",kit
R2144	T2717	T2722	det	the,Kit
R2145	T2718	T2722	compound	Supersignal,Kit
R2146	T2719	T2720	compound	West,Pico
R2147	T2720	T2722	compound	Pico,Kit
R2148	T2721	T2722	compound	Chemiluminescent,Kit
R2149	T2722	T2715	conj	Kit,kit
R2150	T2723	T2722	punct	",",Kit
R2151	T2724	T2722	cc	and,Kit
R2152	T2725	T2729	det	the,Kit
R2153	T2726	T2728	compound	LightShift,EMSA
R2154	T2727	T2728	compound	Chemiluminescent,EMSA
R2155	T2728	T2729	compound	EMSA,Kit
R2156	T2729	T2722	conj	Kit,Kit
R2157	T2730	T2731	auxpass	were,purchased
R2158	T2731	T2731	ROOT	purchased,purchased
R2159	T2732	T2731	prep	from,purchased
R2161	T2734	T2735	punct	(,Rockland
R2162	T2735	T2733	appos	Rockland,Pierce
R2163	T2736	T2733	punct	",",Pierce
R2164	T2737	T2731	prep	IL,purchased
R2165	T2738	T2731	punct	),purchased
R2166	T2739	T2731	punct	.,purchased
R2167	T2740	T2745	nmod	CREB,probes
R2168	T2741	T2740	cc	and,CREB
R2169	T2742	T2740	conj	NF-κB,CREB
R2170	T2743	T2744	compound	DNA,oligonucleotide
R2171	T2744	T2745	compound	oligonucleotide,probes
R2172	T2745	T2747	nsubjpass	probes,synthesized
R2173	T2746	T2747	auxpass	were,synthesized
R2174	T2747	T2747	ROOT	synthesized,synthesized
R2175	T2748	T2747	agent	by,synthesized
R2176	T2749	T2748	pobj	IDT,by
R2177	T2750	T2751	punct	(,Coralville
R2178	T2751	T2749	appos	Coralville,IDT
R2179	T2752	T2751	punct	",",Coralville
R2180	T2753	T2751	appos	IA,Coralville
R2181	T2754	T2751	punct	),Coralville
R2182	T2755	T2747	punct	.,synthesized
R2183	T2756	T2757	compound	Nylon,membranes
R2184	T2757	T2759	nsubjpass	membranes,purchased
R2185	T2758	T2759	auxpass	were,purchased
R2186	T2759	T2759	ROOT	purchased,purchased
R2187	T2760	T2759	prep	from,purchased
R2188	T2761	T2763	compound	Roche,Sciences
R2189	T2762	T2763	compound	Applied,Sciences
R2190	T2763	T2760	pobj	Sciences,from
R2191	T2764	T2763	punct	(,Sciences
R2192	T2765	T2763	appos	Indianapolis,Sciences
R2193	T2766	T2763	punct	",",Sciences
R2194	T2767	T2759	prep	IN,purchased
R2195	T2768	T2767	punct	),IN
R2196	T2769	T2759	punct	.,purchased
R2197	T2770	T2773	amod	Western,quantification
R2198	T2771	T2772	compound	blot,band
R2199	T2772	T2773	compound	band,quantification
R2200	T2773	T2775	nsubjpass	quantification,performed
R2201	T2774	T2775	auxpass	was,performed
R2202	T2775	T2775	ROOT	performed,performed
R2203	T2776	T2775	prep	with,performed
R2204	T2777	T2781	det	the,software
R2205	T2778	T2780	compound	UN-SCAN-IT,Digitizing
R2206	T2779	T2780	compound	Gel,Digitizing
R2207	T2780	T2781	compound	Digitizing,software
R2208	T2781	T2776	pobj	software,with
R2209	T2782	T2784	punct	(,Scientific
R2210	T2783	T2784	compound	Silk,Scientific
R2211	T2784	T2781	appos	Scientific,software
R2212	T2785	T2784	punct	",",Scientific
R2213	T2786	T2784	appos	Orem,Scientific
R2214	T2787	T2786	punct	",",Orem
R2215	T2788	T2786	appos	UT,Orem
R2216	T2789	T2786	punct	),Orem
R2217	T2790	T2775	punct	.,performed
R2600	T3267	T3268	compound	Human,Peripheral
R2601	T3268	T3269	compound	Peripheral,Blood
R2602	T3269	T3272	compound	Blood,Isolation
R2603	T3270	T3272	compound	Mononuclear,Isolation
R2604	T3271	T3272	compound	Cell,Isolation
R2605	T3272	T3279	nsubjpass	Isolation,isolated
R2606	T3273	T3272	cc	and,Isolation
R2607	T3274	T3275	compound	Stimulation,Human
R2608	T3275	T3277	compound	Human,cells
R2609	T3276	T3277	compound	mononuclear,cells
R2610	T3277	T3272	conj	cells,Isolation
R2611	T3278	T3279	auxpass	were,isolated
R2612	T3279	T3279	ROOT	isolated,isolated
R2613	T3280	T3279	prep	from,isolated
R2614	T3281	T3283	det	the,blood
R2615	T3282	T3283	compound	peripheral,blood
R2616	T3283	T3280	pobj	blood,from
R2617	T3284	T3283	prep	of,blood
R2618	T3285	T3288	nummod	four,volunteers
R2619	T3286	T3288	amod	healthy,volunteers
R2620	T3287	T3288	amod	human,volunteers
R2621	T3288	T3284	pobj	volunteers,of
R2622	T3289	T3279	punct	.,isolated
R2623	T3290	T3292	det	The,size
R2624	T3291	T3292	compound	sample,size
R2625	T3292	T3294	nsubjpass	size,chosen
R2626	T3293	T3294	auxpass	was,chosen
R2627	T3294	T3294	ROOT	chosen,chosen
R2628	T3295	T3294	prep	from,chosen
R2629	T3296	T3299	poss	our,work
R2630	T3297	T3298	advmod	previously,published
R2631	T3298	T3299	amod	published,work
R2632	T3299	T3295	pobj	work,from
R2633	T3300	T3299	punct	",",work
R2634	T3301	T3302	nsubj	which,demonstrated
R2635	T3302	T3299	relcl	demonstrated,work
R2636	T3303	T3304	amod	reliable,results
R2637	T3304	T3302	dobj	results,demonstrated
R2638	T3305	T3307	nummod	.10,isolation
R2639	T3306	T3307	compound	Cell,isolation
R2640	T3307	T3313	nsubjpass	isolation,conducted
R2641	T3308	T3307	cc	and,isolation
R2642	T3309	T3311	det	all,experiments
R2643	T3310	T3311	amod	subsequent,experiments
R2644	T3311	T3313	nsubjpass	experiments,conducted
R2645	T3312	T3313	auxpass	were,conducted
R2646	T3313	T3294	advcl	conducted,chosen
R2647	T3314	T3313	prep	under,conducted
R2648	T3315	T3318	amod	sterile,conditions
R2649	T3316	T3315	cc	and,sterile
R2650	T3317	T3315	conj	pyrogen-free,sterile
R2651	T3318	T3314	pobj	conditions,under
R2652	T3319	T3313	punct	.,conducted
R2653	T3320	T3321	nsubj	Blood,collected
R2654	T3321	T3321	ROOT	collected,collected
R2655	T3322	T3321	prep	in,collected
R2656	T3323	T3324	compound	heparin,tubes
R2657	T3324	T3322	pobj	tubes,in
R2658	T3325	T3326	auxpass	was,incubated
R2659	T3326	T3321	conj	incubated,collected
R2660	T3327	T3335	mark	with,sedimented
R2661	T3328	T3335	nsubjpass	Dextran,sedimented
R2662	T3329	T3328	nummod	T500,Dextran
R2663	T3330	T3328	cc	and,Dextran
R2664	T3331	T3333	det	the,cells
R2665	T3332	T3333	amod	red,cells
R2666	T3333	T3328	conj	cells,Dextran
R2667	T3334	T3335	auxpass	were,sedimented
R2668	T3335	T3326	advcl	sedimented,incubated
R2669	T3336	T3335	prep	for,sedimented
R2670	T3337	T3338	nummod	40,minutes
R2671	T3338	T3336	pobj	minutes,for
R2672	T3339	T3338	prep	at,minutes
R2673	T3340	T3341	compound	room,temperature
R2674	T3341	T3339	pobj	temperature,at
R2675	T3342	T3321	punct	.,collected
R2676	T3343	T3345	det	The,serum
R2677	T3344	T3345	amod	resultant,serum
R2678	T3345	T3348	nsubjpass	serum,washed
R2679	T3346	T3348	auxpass	was,washed
R2680	T3347	T3348	advmod	then,washed
R2681	T3348	T3348	ROOT	washed,washed
R2682	T3349	T3348	prep	with,washed
R2683	T3350	T3349	pobj	HBSS,with
R2684	T3351	T3348	cc	and,washed
R2685	T3352	T3348	conj	separated,washed
R2686	T3353	T3352	agent	by,separated
R2687	T3354	T3356	nmod	Percoll,centrifugation
R2688	T3355	T3356	compound	gradient,centrifugation
R2689	T3356	T3353	pobj	centrifugation,by
R2690	T3357	T3348	prep	according,washed
R2691	T3358	T3357	prep	to,according
R2692	T3359	T3360	det	the,manufacturer
R2693	T3360	T3361	poss	manufacturer,instructions
R2694	T3361	T3358	pobj	instructions,to
R2695	T3362	T3348	punct	.,washed
R2696	T3363	T3364	compound	Cell,viability
R2697	T3364	T3366	nsubjpass	viability,assessed
R2698	T3365	T3366	auxpass	was,assessed
R2699	T3366	T3366	ROOT	assessed,assessed
R2700	T3367	T3366	agent	by,assessed
R2701	T3368	T3371	compound	trypan,exclusion
R2702	T3369	T3370	amod	blue,dye
R2703	T3370	T3371	compound	dye,exclusion
R2704	T3371	T3367	pobj	exclusion,by
R2705	T3372	T3371	punct	",",exclusion
R2706	T3373	T3366	prep	with,assessed
R2707	T3374	T3373	pobj	purity,with
R2708	T3375	T3374	prep	of,purity
R2709	T3376	T3378	amod	greater,95
R2710	T3377	T3378	quantmod	than,95
R2711	T3378	T3379	nummod	95,%
R2712	T3379	T3375	pobj	%,of
R2713	T3380	T3366	punct	.,assessed
R2714	T3381	T3382	amod	Isolated,cells
R2715	T3382	T3384	nsubjpass	cells,resuspended
R2716	T3383	T3384	auxpass	were,resuspended
R2717	T3384	T3384	ROOT	resuspended,resuspended
R2718	T3385	T3384	prep	in,resuspended
R2719	T3386	T3388	nsubj	RPMI,supplemented
R2720	T3387	T3386	nummod	1640,RPMI
R2721	T3388	T3384	conj	supplemented,resuspended
R2722	T3389	T3388	prep	with,supplemented
R2723	T3390	T3391	nummod	10,%
R2724	T3391	T3392	compound	%,FBS
R2725	T3392	T3389	pobj	FBS,with
R2726	T3393	T3392	cc	and,FBS
R2727	T3394	T3396	nummod	5,HEPES
R2728	T3395	T3396	compound	mM,HEPES
R2729	T3396	T3392	conj	HEPES,FBS
R2730	T3397	T3388	prep	at,supplemented
R2731	T3398	T3399	det	a,concentration
R2732	T3399	T3397	pobj	concentration,at
R2733	T3400	T3399	prep	of,concentration
R2734	T3401	T3403	nummod	1,107cells/mL
R2735	T3402	T3403	compound	x,107cells/mL
R2736	T3403	T3400	pobj	107cells/mL,of
R2737	T3404	T3388	punct	.,supplemented
R2738	T3405	T3407	det	Each,experiment
R2739	T3406	T3407	amod	subsequent,experiment
R2740	T3407	T3408	nsubj	experiment,listed
R2741	T3408	T3408	ROOT	listed,listed
R2742	T3409	T3408	advmod	below,listed
R2743	T3410	T3411	auxpass	was,conducted
R2744	T3411	T3408	dep	conducted,listed
R2745	T3412	T3411	prep	on,conducted
R2746	T3413	T3412	pobj	samples,on
R2747	T3414	T3413	prep	of,samples
R2748	T3415	T3418	nummod	5,cells
R2749	T3416	T3418	nmod	x,cells
R2750	T3417	T3418	nummod	106,cells
R2751	T3418	T3414	pobj	cells,of
R2752	T3419	T3418	prep	per,cells
R2753	T3420	T3421	compound	treatment,group
R2754	T3421	T3419	pobj	group,per
R2755	T3422	T3408	punct	.,listed
R2756	T3423	T3424	amod	Isolated,cells
R2757	T3424	T3426	nsubjpass	cells,stimulated
R2758	T3425	T3426	auxpass	were,stimulated
R2759	T3426	T3426	ROOT	stimulated,stimulated
R2760	T3427	T3426	prep	with,stimulated
R2761	T3428	T3429	det	either,HBSS
R2762	T3429	T3427	pobj	HBSS,with
R2763	T3430	T3429	prep	as,HBSS
R2764	T3431	T3433	det	a,control
R2765	T3432	T3433	amod	negative,control
R2766	T3433	T3430	pobj	control,as
R2767	T3434	T3433	punct	",",control
R2768	T3435	T3433	appos	LPS,control
R2769	T3436	T3440	punct	(,mL
R2770	T3437	T3438	nummod	1,μg
R2771	T3438	T3440	compound	μg,mL
R2772	T3439	T3440	compound	/,mL
R2773	T3440	T3435	appos	mL,LPS
R2774	T3441	T3440	punct	),mL
R2775	T3442	T3435	punct	",",LPS
R2776	T3443	T3454	csubj	PTX,","
R2777	T3444	T3446	punct	(,mM
R2778	T3445	T3446	nummod	20,mM
R2779	T3446	T3443	parataxis	mM,PTX
R2780	T3447	T3446	punct	),mM
R2781	T3448	T3446	punct	",",mM
R2782	T3449	T3443	cc	or,PTX
R2783	T3450	T3452	amod	concomitant,/
R2784	T3451	T3452	compound	LPS,/
R2785	T3452	T3453	compound	/,PTX
R2786	T3453	T3443	conj	PTX,PTX
R2787	T3454	T3435	punct	",",LPS
R2788	T3455	T3433	prep	at,control
R2789	T3456	T3458	det	the,concentrations
R2790	T3457	T3458	amod	above-mentioned,concentrations
R2791	T3458	T3455	pobj	concentrations,at
R2792	T3459	T3426	punct	",",stimulated
R2793	T3460	T3426	prep	for,stimulated
R2794	T3461	T3462	nummod	30,minutes
R2795	T3462	T3460	pobj	minutes,for
R2796	T3463	T3462	prep	at,minutes
R2797	T3464	T3463	pobj	37ºC,at
R2798	T3465	T3426	punct	.,stimulated
R2799	T3466	T3468	nsubjpass	Cells,placed
R2800	T3467	T3468	auxpass	were,placed
R2801	T3468	T3468	ROOT	placed,placed
R2802	T3469	T3468	prep	on,placed
R2803	T3470	T3469	pobj	ice,on
R2804	T3471	T3468	prep	for,placed
R2805	T3472	T3473	nummod	10,minutes
R2806	T3473	T3471	pobj	minutes,for
R2807	T3474	T3475	aux	to,stop
R2808	T3475	T3468	advcl	stop,placed
R2809	T3476	T3477	det	the,reaction
R2810	T3477	T3475	dobj	reaction,stop
R2811	T3478	T3477	cc	and,reaction
R2812	T3479	T3480	det	the,samples
R2813	T3480	T3483	nsubjpass	samples,stored
R2814	T3481	T3483	auxpass	were,stored
R2815	T3482	T3483	advmod	then,stored
R2816	T3483	T3468	conj	stored,placed
R2817	T3484	T3483	prep	at,stored
R2818	T3485	T3488	nummod	−,C
R2819	T3486	T3487	nummod	70,°
R2820	T3487	T3488	compound	°,C
R2821	T3488	T3484	pobj	C,at
R2822	T3489	T3483	prep	for,stored
R2823	T3490	T3491	amod	further,analysis
R2824	T3491	T3489	pobj	analysis,for
R2825	T3492	T3483	punct	.,stored
R2826	T3493	T3495	det	The,times
R2827	T3494	T3495	compound	stimulation,times
R2828	T3495	T3502	nsubj	times,utilized
R2829	T3496	T3495	cc	and,times
R2830	T3497	T3495	conj	concentrations,times
R2831	T3498	T3497	prep	of,concentrations
R2832	T3499	T3498	pobj	LPS,of
R2833	T3500	T3499	cc	and,LPS
R2834	T3501	T3499	conj	PTX,LPS
R2835	T3502	T3502	ROOT	utilized,utilized
R2836	T3503	T3502	prep	in,utilized
R2837	T3504	T3505	det	this,study
R2838	T3505	T3503	pobj	study,in
R2839	T3506	T3507	auxpass	were,determined
R2840	T3507	T3502	conj	determined,utilized
R2841	T3508	T3507	prep	in,determined
R2842	T3509	T3511	amod	previous,studies
R2843	T3510	T3511	compound	pilot,studies
R2844	T3511	T3508	pobj	studies,in
R2845	T3512	T3511	acl	done,studies
R2846	T3513	T3512	prep	in,done
R2847	T3514	T3515	poss	our,laboratory
R2848	T3515	T3513	pobj	laboratory,in
R2849	T3516	T3515	punct	",",laboratory
R2850	T3517	T3518	nsubj	which,examined
R2851	T3518	T3511	relcl	examined,studies
R2852	T3519	T3520	det	the,effects
R2853	T3520	T3518	dobj	effects,examined
R2854	T3521	T3520	prep	of,effects
R2855	T3522	T3521	pcomp	increasing,of
R2856	T3523	T3522	dobj	concentrations,increasing
R2857	T3524	T3523	prep	of,concentrations
R2858	T3525	T3524	pobj	PTX,of
R2859	T3526	T3523	prep	on,concentrations
R2860	T3527	T3529	amod	LPS-induced,production
R2861	T3528	T3529	compound	TNF-α,production
R2862	T3529	T3526	pobj	production,on
R2863	T3530	T3502	punct	.,utilized
R2864	T3531	T3548	nsubj	LPS,were
R2865	T3532	T3531	prep	at,LPS
R2866	T3533	T3534	det	a,concentration
R2867	T3534	T3532	pobj	concentration,at
R2868	T3535	T3534	prep	of,concentration
R2869	T3536	T3537	nummod	1,μg
R2870	T3537	T3539	compound	μg,mL
R2871	T3538	T3539	compound	/,mL
R2872	T3539	T3535	pobj	mL,of
R2873	T3540	T3539	cc	and,mL
R2874	T3541	T3539	conj	PTX,mL
R2875	T3542	T3534	prep	at,concentration
R2876	T3543	T3544	det	a,concentration
R2877	T3544	T3542	pobj	concentration,at
R2878	T3545	T3544	prep	of,concentration
R2879	T3546	T3547	nummod	20,mM
R2880	T3547	T3545	pobj	mM,of
R2881	T3548	T3548	ROOT	were,were
R2882	T3549	T3551	det	the,concentrations
R2883	T3550	T3551	amod	minimum,concentrations
R2884	T3551	T3548	attr	concentrations,were
R2885	T3552	T3551	amod	necessary,concentrations
R2886	T3553	T3554	aux	to,produce
R2887	T3554	T3552	xcomp	produce,necessary
R2888	T3555	T3559	det	a,up-regulation
R2889	T3556	T3559	amod	reproducible,up-regulation
R2890	T3557	T3556	cc	and,reproducible
R2891	T3558	T3556	conj	reliable,reproducible
R2892	T3559	T3554	dobj	up-regulation,produce
R2893	T3560	T3559	cc	and,up-regulation
R2894	T3561	T3559	conj	down-regulation,up-regulation
R2895	T3562	T3559	prep	in,up-regulation
R2896	T3563	T3564	compound	TNF-α,production
R2897	T3564	T3562	pobj	production,in
R2898	T3565	T3559	punct	",",up-regulation
R2899	T3566	T3554	advmod	respectively,produce
R2900	T3567	T3554	punct	",",produce
R2901	T3568	T3554	prep	in,produce
R2902	T3569	T3570	amod	quantitative,assays
R2903	T3570	T3568	pobj	assays,in
R2904	T3571	T3574	punct	(,shown
R2905	T3572	T3574	nsubjpass	data,shown
R2906	T3573	T3574	neg	not,shown
R2907	T3574	T3548	parataxis	shown,were
R2908	T3575	T3574	punct	),shown
R2909	T3576	T3548	punct	.,were
R3250	T3971	T3971	ROOT	Effect,Effect
R3251	T3972	T3971	prep	of,Effect
R3252	T3973	T3972	pobj	PTX,of
R3253	T3974	T3971	prep	on,Effect
R3254	T3975	T3977	amod	LPS-induced,production
R3255	T3976	T3977	compound	TNF-α,production
R3256	T3977	T3974	pobj	production,on
R3257	T3978	T3977	prep	by,production
R3258	T3979	T3980	compound	mononuclear,cells
R3259	T3980	T3978	pobj	cells,by
R3260	T3981	T3982	det	The,concentration
R3261	T3982	T3987	nsubjpass	concentration,determined
R3262	T3983	T3982	prep	of,concentration
R3263	T3984	T3983	pobj	TNF-α,of
R3264	T3985	T3987	auxpass	was,determined
R3265	T3986	T3987	advmod	quantitatively,determined
R3266	T3987	T3987	ROOT	determined,determined
R3267	T3988	T3987	agent	by,determined
R3268	T3989	T3988	pobj	ELISA,by
R3269	T3990	T3987	prep	in,determined
R3270	T3991	T3992	det	the,media
R3271	T3992	T3990	pobj	media,in
R3272	T3993	T3992	prep	of,media
R3273	T3994	T3993	pobj	cells,of
R3274	T3995	T3994	acl	exposed,cells
R3275	T3996	T3995	prep	to,exposed
R3276	T3997	T3998	det	the,treatments
R3277	T3998	T3996	pobj	treatments,to
R3278	T3999	T3998	acl	described,treatments
R3279	T4000	T3999	advmod	above,described
R3280	T4001	T3999	prep	after,described
R3281	T4002	T4001	pobj	stimulation,after
R3282	T4003	T3999	prep	for,described
R3283	T4004	T4007	nmod	up,maximum
R3284	T4005	T4007	quantmod	to,maximum
R3285	T4006	T4007	det	a,maximum
R3286	T4007	T4003	pobj	maximum,for
R3287	T4008	T4007	prep	of,maximum
R3288	T4009	T4010	nummod	18,hours
R3289	T4010	T4008	pobj	hours,of
R3290	T4011	T3999	punct	",",described
R3291	T4012	T3999	prep	at,described
R3292	T4013	T4014	amod	3-hour,intervals
R3293	T4014	T4012	pobj	intervals,at
R3294	T4015	T3987	punct	.,determined
R3295	T4016	T4017	det	The,results
R3296	T4017	T4019	nsubjpass	results,expressed
R3297	T4018	T4019	auxpass	are,expressed
R3298	T4019	T4019	ROOT	expressed,expressed
R3299	T4020	T4019	prep	in,expressed
R3300	T4021	T4020	pobj	pg/mL,in
R3301	T4022	T4019	punct	.,expressed
R3373	T4152	T4152	ROOT	SDS-PAGE,SDS-PAGE
R3374	T4153	T4152	cc	and,SDS-PAGE
R3375	T4154	T4152	conj	Immunoblotting,SDS-PAGE
R3376	T4155	T4156	punct	(,I-κBα
R3377	T4156	T4152	conj	I-κBα,SDS-PAGE
R3378	T4157	T4156	punct	",",I-κBα
R3379	T4158	T4156	conj	NF-κB,I-κBα
R3380	T4159	T4156	appos	p65,I-κBα
R3381	T4160	T4156	punct	",",I-κBα
R3382	T4161	T4156	cc	and,I-κBα
R3383	T4162	T4156	conj	CREB,I-κBα
R3384	T4163	T4156	punct	),I-κBα
R3385	T4164	T4169	prep	Following,washed
R3386	T4165	T4164	pobj	stimulation,Following
R3387	T4166	T4169	punct	",",washed
R3388	T4167	T4169	nsubjpass	PBMCs,washed
R3389	T4168	T4169	auxpass	were,washed
R3390	T4169	T4169	ROOT	washed,washed
R3391	T4170	T4169	prep	with,washed
R3392	T4171	T4172	amod	ice-cold,PBS
R3393	T4172	T4170	pobj	PBS,with
R3394	T4173	T4169	cc	and,washed
R3395	T4174	T4169	conj	centrifuged,washed
R3396	T4175	T4176	aux	to,collect
R3397	T4176	T4174	advcl	collect,centrifuged
R3398	T4177	T4179	det	a,pellet
R3399	T4178	T4179	compound	cell,pellet
R3400	T4179	T4176	dobj	pellet,collect
R3401	T4180	T4169	punct	.,washed
R3402	T4181	T4182	det	The,pellet
R3403	T4182	T4184	nsubjpass	pellet,resuspended
R3404	T4183	T4184	auxpass	was,resuspended
R3405	T4184	T4184	ROOT	resuspended,resuspended
R3406	T4185	T4184	prep	in,resuspended
R3407	T4186	T4189	amod	ice-cold,buffer
R3408	T4187	T4189	compound	SDS,buffer
R3409	T4188	T4189	compound	sample,buffer
R3410	T4189	T4185	pobj	buffer,in
R3411	T4190	T4184	conj	supplemented,resuspended
R3412	T4191	T4190	prep	with,supplemented
R3413	T4192	T4194	nummod	100,dithiothreitol
R3414	T4193	T4194	compound	mM,dithiothreitol
R3415	T4194	T4191	pobj	dithiothreitol,with
R3416	T4195	T4184	punct	.,resuspended
R3417	T4196	T4200	nsubj	Cytoplasmic,were
R3418	T4197	T4196	cc	and,Cytoplasmic
R3419	T4198	T4199	amod	nuclear,extracts
R3420	T4199	T4196	conj	extracts,Cytoplasmic
R3421	T4200	T4200	ROOT	were,were
R3422	T4201	T4200	acomp	isolated,were
R3423	T4202	T4201	prep	with,isolated
R3424	T4203	T4208	compound	NE-PER,reagents
R3425	T4204	T4207	amod	nuclear,extraction
R3426	T4205	T4204	cc	and,nuclear
R3427	T4206	T4204	conj	cytoplasmic,nuclear
R3428	T4207	T4208	compound	extraction,reagents
R3429	T4208	T4202	pobj	reagents,with
R3430	T4209	T4208	prep	with,reagents
R3431	T4210	T4214	nummod	1x,Cocktail
R3432	T4211	T4214	compound	Halt,Cocktail
R3433	T4212	T4214	compound	Protease,Cocktail
R3434	T4213	T4214	compound	Inhibitor,Cocktail
R3435	T4214	T4215	nsubj	Cocktail,according
R3436	T4215	T4200	prep	according,were
R3437	T4216	T4215	prep	to,according
R3438	T4217	T4218	det	the,manufacturer
R3439	T4218	T4219	poss	manufacturer,instructions
R3440	T4219	T4216	pobj	instructions,to
R3441	T4220	T4200	punct	.,were
R3442	T4221	T4222	compound	Protein,concentrations
R3443	T4222	T4224	nsubjpass	concentrations,determined
R3444	T4223	T4224	auxpass	were,determined
R3445	T4224	T4224	ROOT	determined,determined
R3446	T4225	T4224	prep	with,determined
R3447	T4226	T4228	det	the,protein
R3448	T4227	T4228	compound	BCA,protein
R3449	T4228	T4230	nmod	protein,kit
R3450	T4229	T4230	compound	reagent,kit
R3451	T4230	T4225	pobj	kit,with
R3452	T4231	T4230	prep	for,kit
R3453	T4232	T4233	det	each,sample
R3454	T4233	T4231	pobj	sample,for
R3455	T4234	T4224	prep	according,determined
R3456	T4235	T4234	prep	to,according
R3457	T4236	T4238	det	a,curve
R3458	T4237	T4238	amod	standardized,curve
R3459	T4238	T4235	pobj	curve,to
R3460	T4239	T4238	prep	for,curve
R3461	T4240	T4239	pobj	albumin,for
R3462	T4241	T4224	punct	.,determined
R3463	T4242	T4243	compound	Cell,lysates
R3464	T4243	T4245	nsubjpass	lysates,collected
R3465	T4244	T4245	auxpass	were,collected
R3466	T4245	T4245	ROOT	collected,collected
R3467	T4246	T4245	prep	by,collected
R3468	T4247	T4246	pcomp	boiling,by
R3469	T4248	T4249	det	the,samples
R3470	T4249	T4247	dobj	samples,boiling
R3471	T4250	T4247	prep	in,boiling
R3472	T4251	T4254	det	a,bath
R3473	T4252	T4254	amod	100ºC,bath
R3474	T4253	T4254	compound	water,bath
R3475	T4254	T4250	pobj	bath,in
R3476	T4255	T4247	prep	for,boiling
R3477	T4256	T4257	nummod	5,minutes
R3478	T4257	T4255	pobj	minutes,for
R3479	T4258	T4245	punct	.,collected
R3480	T4259	T4260	compound	Ten,μg
R3481	T4260	T4266	nsubjpass	μg,separated
R3482	T4261	T4260	prep	of,μg
R3483	T4262	T4261	pobj	protein,of
R3484	T4263	T4262	prep	per,protein
R3485	T4264	T4263	pobj	sample,per
R3486	T4265	T4266	auxpass	was,separated
R3487	T4266	T4266	ROOT	separated,separated
R3488	T4267	T4266	agent	by,separated
R3489	T4268	T4270	amod	SDS-polyacrylamide,electrophoresis
R3490	T4269	T4270	compound	gel,electrophoresis
R3491	T4270	T4267	pobj	electrophoresis,by
R3492	T4271	T4266	prep	through,separated
R3493	T4272	T4271	pobj	8,through
R3494	T4273	T4274	nummod	16,%
R3495	T4274	T4278	nmod	%,gels
R3496	T4275	T4278	amod	tris-glycine,gels
R3497	T4276	T4278	compound	polyacrylamide,gels
R3498	T4277	T4278	compound	gradient,gels
R3499	T4278	T4272	appos	gels,8
R3500	T4279	T4266	cc	and,separated
R3501	T4280	T4266	conj	transferred,separated
R3502	T4281	T4280	prep	to,transferred
R3503	T4282	T4283	amod	nitrocellulose,membranes
R3504	T4283	T4281	pobj	membranes,to
R3505	T4284	T4266	punct	.,separated
R3506	T4285	T4286	det	The,membranes
R3507	T4286	T4288	nsubjpass	membranes,blocked
R3508	T4287	T4288	auxpass	were,blocked
R3509	T4288	T4288	ROOT	blocked,blocked
R3510	T4289	T4288	prep	with,blocked
R3511	T4290	T4291	nummod	5,%
R3512	T4291	T4292	compound	%,milk
R3513	T4292	T4289	pobj	milk,with
R3514	T4293	T4292	prep	in,milk
R3515	T4294	T4295	compound	Tris-buffered,saline/Tween
R3516	T4295	T4293	pobj	saline/Tween,in
R3517	T4296	T4295	nummod	20,saline/Tween
R3518	T4297	T4303	punct	(,PA
R3519	T4298	T4299	compound	Fischer,Scientific
R3520	T4299	T4303	dep	Scientific,PA
R3521	T4300	T4299	punct	",",Scientific
R3522	T4301	T4299	appos	Pittsburgh,Scientific
R3523	T4302	T4301	punct	",",Pittsburgh
R3524	T4303	T4303	ROOT	PA,PA
R3525	T4304	T4303	punct	),PA
R3526	T4305	T4303	prep	for,PA
R3527	T4306	T4307	nummod	1,hour
R3528	T4307	T4305	pobj	hour,for
R3529	T4308	T4303	punct	.,PA
R3530	T4309	T4310	amod	Cytoplasmic,extracts
R3531	T4310	T4312	nsubjpass	extracts,incubated
R3532	T4311	T4312	auxpass	were,incubated
R3533	T4312	T4312	ROOT	incubated,incubated
R3534	T4313	T4312	prep	with,incubated
R3535	T4314	T4316	amod	phosphorylated,antibody
R3536	T4315	T4316	amod	I-κBα,antibody
R3537	T4316	T4313	pobj	antibody,with
R3538	T4317	T4316	punct	(,antibody
R3539	T4318	T4316	appos	1:200,antibody
R3540	T4319	T4316	punct	),antibody
R3541	T4320	T4312	punct	",",incubated
R3542	T4321	T4325	mark	while,incubated
R3543	T4322	T4323	amod	nuclear,extracts
R3544	T4323	T4325	nsubjpass	extracts,incubated
R3545	T4324	T4325	auxpass	were,incubated
R3546	T4325	T4312	advcl	incubated,incubated
R3547	T4326	T4325	prep	with,incubated
R3548	T4327	T4331	det	either,antibody
R3549	T4328	T4331	amod	phosphorylated,antibody
R3550	T4329	T4331	nmod	NF-κB,antibody
R3551	T4330	T4331	nummod	p65,antibody
R3552	T4331	T4326	pobj	antibody,with
R3553	T4332	T4331	punct	(,antibody
R3554	T4333	T4331	appos	1:500,antibody
R3555	T4334	T4331	punct	),antibody
R3556	T4335	T4325	cc	or,incubated
R3557	T4336	T4338	amod	phosphorylated,antibody
R3558	T4337	T4338	compound	CREB,antibody
R3559	T4338	T4346	nmod	antibody,C
R3560	T4339	T4338	punct	(,antibody
R3561	T4340	T4338	appos	1:500,antibody
R3562	T4341	T4338	punct	),antibody
R3563	T4342	T4346	amod	overnight,C
R3564	T4343	T4342	prep	at,overnight
R3565	T4344	T4345	nummod	4,°
R3566	T4345	T4343	pobj	°,at
R3567	T4346	T4325	conj	C,incubated
R3568	T4347	T4346	prep	in,C
R3569	T4348	T4349	amod	separate,experiments
R3570	T4349	T4347	pobj	experiments,in
R3571	T4350	T4312	punct	.,incubated
R3572	T4351	T4352	det	The,membranes
R3573	T4352	T4354	nsubjpass	membranes,washed
R3574	T4353	T4354	auxpass	were,washed
R3575	T4354	T4354	ROOT	washed,washed
R3576	T4355	T4354	prep	with,washed
R3577	T4356	T4357	compound	Tris-buffered,saline/Tween
R3578	T4357	T4355	pobj	saline/Tween,with
R3579	T4358	T4357	nummod	20,saline/Tween
R3580	T4359	T4354	cc	and,washed
R3581	T4360	T4354	conj	incubated,washed
R3582	T4361	T4360	prep	for,incubated
R3583	T4362	T4363	nummod	1,hour
R3584	T4363	T4361	pobj	hour,for
R3585	T4364	T4360	prep	at,incubated
R3586	T4365	T4366	compound	room,temperature
R3587	T4366	T4364	pobj	temperature,at
R3588	T4367	T4360	prep	with,incubated
R3589	T4368	T4370	det	the,antibody
R3590	T4369	T4370	amod	secondary,antibody
R3591	T4370	T4367	pobj	antibody,with
R3592	T4371	T4376	punct	",",diluted
R3593	T4372	T4375	nmod	horseradish,IgG
R3594	T4373	T4375	amod	peroxidase-linked,IgG
R3595	T4374	T4375	compound	anti-rabbit,IgG
R3596	T4375	T4376	nsubj	IgG,diluted
R3597	T4376	T4354	conj	diluted,washed
R3598	T4377	T4376	dobj	1:2000,diluted
R3599	T4378	T4376	prep	in,diluted
R3600	T4379	T4378	pcomp	blocking,in
R3601	T4380	T4379	dobj	solution,blocking
R3602	T4381	T4376	punct	.,diluted
R3603	T4382	T4396	prep	After,applied
R3604	T4383	T4384	amod	repeated,washing
R3605	T4384	T4382	pobj	washing,After
R3606	T4385	T4384	prep	of,washing
R3607	T4386	T4387	det	the,membrane
R3608	T4387	T4385	pobj	membrane,of
R3609	T4388	T4396	punct	",",applied
R3610	T4389	T4394	det	the,Kit
R3611	T4390	T4394	compound	Supersignal,Kit
R3612	T4391	T4392	compound	West,Pico
R3613	T4392	T4394	compound	Pico,Kit
R3614	T4393	T4394	compound	Chemiluminescent,Kit
R3615	T4394	T4396	nsubjpass	Kit,applied
R3616	T4395	T4396	auxpass	was,applied
R3617	T4396	T4396	ROOT	applied,applied
R3618	T4397	T4396	prep	for,applied
R3619	T4398	T4399	compound	antibody,detection
R3620	T4399	T4397	pobj	detection,for
R3621	T4400	T4399	prep	per,detection
R3622	T4401	T4402	det	the,manufacturer
R3623	T4402	T4403	poss	manufacturer,instructions
R3624	T4403	T4400	pobj	instructions,per
R3625	T4404	T4396	punct	.,applied
R3626	T4405	T4407	amod	Western,data
R3627	T4406	T4407	compound	blot,data
R3628	T4407	T4409	nsubjpass	data,presented
R3629	T4408	T4409	auxpass	is,presented
R3630	T4409	T4409	ROOT	presented,presented
R3631	T4410	T4409	prep	as,presented
R3632	T4411	T4412	det	a,percentage
R3633	T4412	T4410	pobj	percentage,as
R3634	T4413	T4412	prep	of,percentage
R3635	T4414	T4415	compound	LPS,stimulation
R3636	T4415	T4413	pobj	stimulation,of
R3637	T4416	T4409	punct	.,presented
R3638	T4417	T4418	det	The,percentage
R3639	T4418	T4423	nsubjpass	percentage,calculated
R3640	T4419	T4418	prep	of,percentage
R3641	T4420	T4421	compound	LPS,stimulation
R3642	T4421	T4419	pobj	stimulation,of
R3643	T4422	T4423	auxpass	was,calculated
R3644	T4423	T4423	ROOT	calculated,calculated
R3645	T4424	T4423	prep	by,calculated
R3646	T4425	T4424	pcomp	dividing,by
R3647	T4426	T4430	det	the,total
R3648	T4427	T4430	amod	mean,total
R3649	T4428	T4429	compound	band,pixel
R3650	T4429	T4430	compound	pixel,total
R3651	T4430	T4425	dobj	total,dividing
R3652	T4431	T4430	prep	for,total
R3653	T4432	T4434	det	each,arm
R3654	T4433	T4434	compound	treatment,arm
R3655	T4434	T4431	pobj	arm,for
R3656	T4435	T4430	acl	divided,total
R3657	T4436	T4435	agent	by,divided
R3658	T4437	T4440	det	the,total
R3659	T4438	T4440	amod	mean,total
R3660	T4439	T4440	amod	pixel,total
R3661	T4440	T4436	pobj	total,by
R3662	T4441	T4440	prep	of,total
R3663	T4442	T4441	pobj	samples,of
R3664	T4443	T4442	acl	stimulated,samples
R3665	T4444	T4443	prep	with,stimulated
R3666	T4445	T4444	pobj	LPS,with
R3667	T4446	T4443	cc	and,stimulated
R3668	T4447	T4425	advcl	multiplying,dividing
R3669	T4448	T4447	prep	by,multiplying
R3670	T4449	T4448	pobj	100,by
R3671	T4450	T4423	punct	.,calculated
R3672	T4451	T4456	advmod	Thus,reported
R3673	T4452	T4456	punct	",",reported
R3674	T4453	T4454	compound	LPS,stimulation
R3675	T4454	T4456	nsubjpass	stimulation,reported
R3676	T4455	T4456	auxpass	is,reported
R3677	T4456	T4456	ROOT	reported,reported
R3678	T4457	T4456	prep	as,reported
R3679	T4458	T4459	nummod	100,%
R3680	T4459	T4457	pobj	%,as
R3681	T4460	T4456	cc	and,reported
R3682	T4461	T4467	nsubjpass	each,reported
R3683	T4462	T4461	prep	of,each
R3684	T4463	T4465	det	the,arms
R3685	T4464	T4465	compound	treatment,arms
R3686	T4465	T4462	pobj	arms,of
R3687	T4466	T4467	auxpass	is,reported
R3688	T4467	T4456	conj	reported,reported
R3689	T4468	T4467	prep	as,reported
R3690	T4469	T4470	det	a,percent
R3691	T4470	T4468	pobj	percent,as
R3692	T4471	T4470	prep	of,percent
R3693	T4472	T4473	compound	LPS,stimulation
R3694	T4473	T4471	pobj	stimulation,of
R3695	T4474	T4467	punct	.,reported
R4034	T4907	T4907	ROOT	NF-κB,NF-κB
R4035	T4908	T4907	cc	and,NF-κB
R4036	T4909	T4913	compound	CREB,Assay
R4037	T4910	T4913	compound	Electrophoretic,Assay
R4038	T4911	T4912	compound	Mobility,Shift
R4039	T4912	T4913	compound	Shift,Assay
R4040	T4913	T4907	conj	Assay,NF-κB
R4041	T4914	T4915	punct	(,EMSA
R4042	T4915	T4913	appos	EMSA,Assay
R4043	T4916	T4913	punct	),Assay
R4044	T4917	T4922	det	The,Kit
R4045	T4918	T4922	amod	non-radioactive,Kit
R4046	T4919	T4921	compound	LightShift,EMSA
R4047	T4920	T4921	compound	Chemiluminescent,EMSA
R4048	T4921	T4922	compound	EMSA,Kit
R4049	T4922	T4924	nsubjpass	Kit,used
R4050	T4923	T4924	auxpass	was,used
R4051	T4924	T4924	ROOT	used,used
R4052	T4925	T4926	aux	to,detect
R4053	T4926	T4924	xcomp	detect,used
R4054	T4927	T4928	amod	DNA-protein,interactions
R4055	T4928	T4926	dobj	interactions,detect
R4056	T4929	T4924	punct	.,used
R4057	T4930	T4934	det	The,biotin
R4058	T4931	T4934	nmod	NF-κB,biotin
R4059	T4932	T4933	nummod	3,′
R4060	T4933	T4934	compound	′,biotin
R4061	T4934	T4935	nsubj	biotin,end-labeled
R4062	T4935	T4935	ROOT	end-labeled,end-labeled
R4063	T4936	T4937	compound	DNA,oligonucleotide
R4064	T4937	T4945	nsubj	oligonucleotide,was
R4065	T4938	T4937	acl	used,oligonucleotide
R4066	T4939	T4938	prep	as,used
R4067	T4940	T4941	det	a,probe
R4068	T4941	T4939	pobj	probe,as
R4069	T4942	T4941	prep	for,probe
R4070	T4943	T4944	det	the,EMSA
R4071	T4944	T4942	pobj	EMSA,for
R4072	T4945	T4935	ccomp	was,end-labeled
R4073	T4946	T4950	det	a,construct
R4074	T4947	T4949	amod	42-bp,stranded
R4075	T4948	T4949	amod	double,stranded
R4076	T4949	T4950	amod	stranded,construct
R4077	T4950	T4945	attr	construct,was
R4078	T4951	T4955	punct	(,TTGTTACAA-GGGGACTTTCCGCTGGGGACTTTCCAGGGAGGC
R4079	T4952	T4955	nummod	5,TTGTTACAA-GGGGACTTTCCGCTGGGGACTTTCCAGGGAGGC
R4080	T4953	T4955	punct	′,TTGTTACAA-GGGGACTTTCCGCTGGGGACTTTCCAGGGAGGC
R4081	T4954	T4955	punct	-,TTGTTACAA-GGGGACTTTCCGCTGGGGACTTTCCAGGGAGGC
R4082	T4955	T4959	meta	TTGTTACAA-GGGGACTTTCCGCTGGGGACTTTCCAGGGAGGC,containing
R4083	T4956	T4957	nummod	3,′
R4084	T4957	T4955	appos	′,TTGTTACAA-GGGGACTTTCCGCTGGGGACTTTCCAGGGAGGC
R4085	T4958	T4955	punct	),TTGTTACAA-GGGGACTTTCCGCTGGGGACTTTCCAGGGAGGC
R4086	T4959	T4950	acl	containing,construct
R4087	T4960	T4965	nummod	two,sites
R4088	T4961	T4965	amod	tandemly,sites
R4089	T4962	T4963	amod	repeated,NF-κB
R4090	T4963	T4965	amod	NF-κB,sites
R4091	T4964	T4965	amod	binding,sites
R4092	T4965	T4959	dobj	sites,containing
R4093	T4966	T4965	punct	(,sites
R4094	T4967	T4965	acl	underlined,sites
R4095	T4968	T4965	punct	),sites
R4096	T4969	T4945	punct	.,was
R4097	T4970	T4972	nsubjpass	Specificity,determined
R4098	T4971	T4972	auxpass	was,determined
R4099	T4972	T4972	ROOT	determined,determined
R4100	T4973	T4972	agent	by,determined
R4101	T4974	T4976	det	a,assay
R4102	T4975	T4976	compound	competition,assay
R4103	T4976	T4973	pobj	assay,by
R4104	T4977	T4976	prep	with,assay
R4105	T4978	T4979	det	the,addition
R4106	T4979	T4977	pobj	addition,with
R4107	T4980	T4979	prep	of,addition
R4108	T4981	T4983	nummod	200,excess
R4109	T4982	T4983	amod	molar,excess
R4110	T4983	T4980	pobj	excess,of
R4111	T4984	T4983	prep	of,excess
R4112	T4985	T4989	amod	unlabeled,oligonucleotide
R4113	T4986	T4989	amod	double,oligonucleotide
R4114	T4987	T4989	amod	stranded,oligonucleotide
R4115	T4988	T4989	compound	NF-κB,oligonucleotide
R4116	T4989	T4984	pobj	oligonucleotide,of
R4117	T4990	T4972	punct	.,determined
R4118	T4991	T4995	det	The,biotin
R4119	T4992	T4995	nmod	CREB,biotin
R4120	T4993	T4994	nummod	3,′
R4121	T4994	T4995	compound	′,biotin
R4122	T4995	T4996	nsubj	biotin,end-labeled
R4123	T4996	T4996	ROOT	end-labeled,end-labeled
R4124	T4997	T4998	compound	DNA,oligonucleotide
R4125	T4998	T4999	nsubj	oligonucleotide,used
R4126	T4999	T4996	conj	used,end-labeled
R4127	T5000	T4999	prep	as,used
R4128	T5001	T5002	det	a,probe
R4129	T5002	T5000	pobj	probe,as
R4130	T5003	T5002	prep	for,probe
R4131	T5004	T5005	det	the,EMSA
R4132	T5005	T5003	pobj	EMSA,for
R4133	T5006	T4996	conj	was,end-labeled
R4134	T5007	T5011	det	a,construct
R4135	T5008	T5010	amod	23-bp,stranded
R4136	T5009	T5010	amod	double,stranded
R4137	T5010	T5011	amod	stranded,construct
R4138	T5011	T5006	attr	construct,was
R4139	T5012	T5019	punct	(,TCTGACGTCAGA-GC
R4140	T5013	T5019	nummod	5,TCTGACGTCAGA-GC
R4141	T5014	T5019	punct	′,TCTGACGTCAGA-GC
R4142	T5015	T5019	punct	-,TCTGACGTCAGA-GC
R4143	T5016	T5019	compound	TTT,TCTGACGTCAGA-GC
R4144	T5017	T5019	compound	TCG,TCTGACGTCAGA-GC
R4145	T5018	T5019	compound	AGC,TCTGACGTCAGA-GC
R4146	T5019	T5011	appos	TCTGACGTCAGA-GC,construct
R4147	T5020	T5021	nummod	3,′
R4148	T5021	T5019	appos	′,TCTGACGTCAGA-GC
R4149	T5022	T5021	punct	),′
R4150	T5023	T5019	acl	containing,TCTGACGTCAGA-GC
R4151	T5024	T5027	det	the,sequence
R4152	T5025	T5027	compound	CRE,sequence
R4153	T5026	T5027	compound	consensus,sequence
R4154	T5027	T5023	dobj	sequence,containing
R4155	T5028	T5029	punct	(,underlined
R4156	T5029	T5019	parataxis	underlined,TCTGACGTCAGA-GC
R4157	T5030	T5029	punct	),underlined
R4158	T5031	T4996	punct	.,end-labeled
R4159	T5032	T5034	nsubjpass	Specificity,determined
R4160	T5033	T5034	auxpass	was,determined
R4161	T5034	T5034	ROOT	determined,determined
R4162	T5035	T5034	agent	by,determined
R4163	T5036	T5038	det	a,assay
R4164	T5037	T5038	compound	competition,assay
R4165	T5038	T5035	pobj	assay,by
R4166	T5039	T5038	prep	with,assay
R4167	T5040	T5041	det	the,addition
R4168	T5041	T5039	pobj	addition,with
R4169	T5042	T5041	prep	of,addition
R4170	T5043	T5045	nummod	200,excess
R4171	T5044	T5045	amod	molar,excess
R4172	T5045	T5042	pobj	excess,of
R4173	T5046	T5045	prep	of,excess
R4174	T5047	T5048	amod	unlabeled,double
R4175	T5048	T5051	amod	double,oligonucleotide
R4176	T5049	T5051	amod	stranded,oligonucleotide
R4177	T5050	T5051	compound	CREB,oligonucleotide
R4178	T5051	T5046	pobj	oligonucleotide,of
R4179	T5052	T5034	punct	.,determined
R4180	T5053	T5054	compound	Nuclear,extracts
R4181	T5054	T5060	nsubjpass	extracts,incubated
R4182	T5055	T5054	punct	(,extracts
R4183	T5056	T5057	nummod	10,μg
R4184	T5057	T5054	appos	μg,extracts
R4185	T5058	T5054	punct	),extracts
R4186	T5059	T5060	auxpass	were,incubated
R4187	T5060	T5060	ROOT	incubated,incubated
R4188	T5061	T5060	prep	with,incubated
R4189	T5062	T5064	nummod	5,NF-κB
R4190	T5063	T5064	compound	nM,NF-κB
R4191	T5064	T5061	pobj	NF-κB,with
R4192	T5065	T5064	cc	or,NF-κB
R4193	T5066	T5067	compound	CREB,probe
R4194	T5067	T5064	conj	probe,NF-κB
R4195	T5068	T5150	punct	(,electrophoresed
R4196	T5069	T5150	nsubjpass	NF-kB,electrophoresed
R4197	T5070	T5069	punct	:,NF-kB
R4198	T5071	T5073	nummod	1x,buffer
R4199	T5072	T5073	amod	binding,buffer
R4200	T5073	T5069	appos	buffer,NF-kB
R4201	T5074	T5073	punct	",",buffer
R4202	T5075	T5077	nummod	50,KCl
R4203	T5076	T5077	compound	mM,KCl
R4204	T5077	T5073	appos	KCl,buffer
R4205	T5078	T5077	punct	",",KCl
R4206	T5079	T5081	nummod	1,EDTA
R4207	T5080	T5081	compound	mM,EDTA
R4208	T5081	T5077	appos	EDTA,KCl
R4209	T5082	T5081	punct	",",EDTA
R4210	T5083	T5085	nummod	1,DTT
R4211	T5084	T5085	compound	mM,DTT
R4212	T5085	T5081	appos	DTT,EDTA
R4213	T5086	T5085	punct	",",DTT
R4214	T5087	T5088	nummod	0.1,%
R4215	T5088	T5085	appos	%,DTT
R4216	T5089	T5093	nummod	NP40,glycerol
R4217	T5090	T5093	punct	",",glycerol
R4218	T5091	T5092	nummod	10,%
R4219	T5092	T5093	compound	%,glycerol
R4220	T5093	T5085	conj	glycerol,DTT
R4221	T5094	T5093	punct	",",glycerol
R4222	T5095	T5093	cc	and,glycerol
R4223	T5096	T5097	nummod	50,ng
R4224	T5097	T5101	amod	ng,dI-dC
R4225	T5098	T5101	nmod	/,dI-dC
R4226	T5099	T5101	compound	μl,dI-dC
R4227	T5100	T5101	compound	poly,dI-dC
R4228	T5101	T5093	conj	dI-dC,glycerol
R4229	T5102	T5101	punct	",",dI-dC
R4230	T5103	T5111	nmod	CREB,Tris
R4231	T5104	T5107	punct	:,buffer
R4232	T5105	T5107	amod	1x,buffer
R4233	T5106	T5107	amod	binding,buffer
R4234	T5107	T5111	nmod	buffer,Tris
R4235	T5108	T5111	punct	",",Tris
R4236	T5109	T5111	nummod	20,Tris
R4237	T5110	T5111	compound	mM,Tris
R4238	T5111	T5101	appos	Tris,dI-dC
R4239	T5112	T5111	punct	",",Tris
R4240	T5113	T5111	conj	pH,Tris
R4241	T5114	T5113	nummod	7.5,pH
R4242	T5115	T5113	punct	",",pH
R4243	T5116	T5118	nummod	50,KCl
R4244	T5117	T5118	compound	mM,KCl
R4245	T5118	T5113	conj	KCl,pH
R4246	T5119	T5118	punct	",",KCl
R4247	T5120	T5122	nummod	1,EDTA
R4248	T5121	T5122	compound	mM,EDTA
R4249	T5122	T5118	conj	EDTA,KCl
R4250	T5123	T5122	punct	",",EDTA
R4251	T5124	T5126	nummod	1,DTT
R4252	T5125	T5126	compound	mM,DTT
R4253	T5126	T5122	conj	DTT,EDTA
R4254	T5127	T5126	punct	",",DTT
R4255	T5128	T5129	nummod	0.10,%
R4256	T5129	T5126	appos	%,DTT
R4257	T5130	T5134	nummod	NP40,glycerol
R4258	T5131	T5130	punct	",",NP40
R4259	T5132	T5133	nummod	6,%
R4260	T5133	T5134	compound	%,glycerol
R4261	T5134	T5126	conj	glycerol,DTT
R4262	T5135	T5134	punct	",",glycerol
R4263	T5136	T5138	nummod	0.1,BSA
R4264	T5137	T5138	compound	mg/mL,BSA
R4265	T5138	T5134	conj	BSA,glycerol
R4266	T5139	T5138	cc	and,BSA
R4267	T5140	T5141	nummod	50,ng
R4268	T5141	T5145	nmod	ng,dI-dC
R4269	T5142	T5145	nmod	/,dI-dC
R4270	T5143	T5145	nmod	μl,dI-dC
R4271	T5144	T5145	compound	poly,dI-dC
R4272	T5145	T5138	conj	dI-dC,BSA
R4273	T5146	T5145	punct	),dI-dC
R4274	T5147	T5101	cc	and,dI-dC
R4275	T5148	T5150	auxpass	were,electrophoresed
R4276	T5149	T5150	advmod	then,electrophoresed
R4277	T5150	T5150	ROOT	electrophoresed,electrophoresed
R4278	T5151	T5150	prep	through,electrophoresed
R4279	T5152	T5157	det	a,gel
R4280	T5153	T5154	nummod	6,%
R4281	T5154	T5157	compound	%,gel
R4282	T5155	T5156	compound	DNA,retardation
R4283	T5156	T5157	compound	retardation,gel
R4284	T5157	T5151	pobj	gel,through
R4285	T5158	T5157	prep	at,gel
R4286	T5159	T5158	pobj	100V,at
R4287	T5160	T5150	prep	for,electrophoresed
R4288	T5161	T5162	nummod	90,minutes
R4289	T5162	T5160	pobj	minutes,for
R4290	T5163	T5150	punct	.,electrophoresed
R4291	T5164	T5165	det	The,gels
R4292	T5165	T5168	nsubjpass	gels,transferred
R4293	T5166	T5168	auxpass	were,transferred
R4294	T5167	T5168	advmod	electrophoretically,transferred
R4295	T5168	T5168	ROOT	transferred,transferred
R4296	T5169	T5168	prep	at,transferred
R4297	T5170	T5169	pobj	380mA,at
R4298	T5171	T5168	prep	for,transferred
R4299	T5172	T5173	nummod	1,hour
R4300	T5173	T5171	pobj	hour,for
R4301	T5174	T5173	prep	on,hour
R4302	T5175	T5174	pobj	ice,on
R4303	T5176	T5168	prep	to,transferred
R4304	T5177	T5181	det	a,membrane
R4305	T5178	T5179	advmod	positively,charged
R4306	T5179	T5181	amod	charged,membrane
R4307	T5180	T5181	compound	nylon,membrane
R4308	T5181	T5176	pobj	membrane,to
R4309	T5182	T5181	cc	and,membrane
R4310	T5183	T5184	advmod	immediately,cross-linked
R4311	T5184	T5181	conj	cross-linked,membrane
R4312	T5185	T5184	prep	for,cross-linked
R4313	T5186	T5187	nummod	15,minutes
R4314	T5187	T5185	pobj	minutes,for
R4315	T5188	T5187	prep	with,minutes
R4316	T5189	T5191	det	a,transilluminator
R4317	T5190	T5191	compound	UV,transilluminator
R4318	T5191	T5188	pobj	transilluminator,with
R4319	T5192	T5187	acl	equipped,minutes
R4320	T5193	T5192	prep	with,equipped
R4321	T5194	T5197	det	a,bulb
R4322	T5195	T5197	nummod	312,bulb
R4323	T5196	T5197	compound	nm,bulb
R4324	T5197	T5193	pobj	bulb,with
R4325	T5198	T5168	punct	.,transferred
R4326	T5199	T5201	amod	Streptavidin-horseradish,conjugate
R4327	T5200	T5201	compound	peroxidase,conjugate
R4328	T5201	T5208	nsubjpass	conjugate,used
R4329	T5202	T5201	cc	and,conjugate
R4330	T5203	T5206	det	the,Substrate
R4331	T5204	T5206	compound	LightShift,Substrate
R4332	T5205	T5206	compound	Chemiluminescent,Substrate
R4333	T5206	T5201	conj	Substrate,conjugate
R4334	T5207	T5208	auxpass	were,used
R4335	T5208	T5208	ROOT	used,used
R4336	T5209	T5210	aux	to,detect
R4337	T5210	T5208	xcomp	detect,used
R4338	T5211	T5212	det	the,biotin
R4339	T5212	T5210	dobj	biotin,detect
R4340	T5213	T5214	compound	end-labeled,DNA
R4341	T5214	T5208	npadvmod	DNA,used
R4342	T5215	T5208	punct	.,used
R4343	T5216	T5218	det	The,membranes
R4344	T5217	T5218	compound	nylon,membranes
R4345	T5218	T5220	nsubjpass	membranes,exposed
R4346	T5219	T5220	auxpass	were,exposed
R4347	T5220	T5220	ROOT	exposed,exposed
R4348	T5221	T5222	aux	to,x-ray
R4349	T5222	T5220	advcl	x-ray,exposed
R4350	T5223	T5222	dobj	film,x-ray
R4351	T5224	T5222	prep	for,x-ray
R4352	T5225	T5226	quantmod	1,3
R4353	T5226	T5227	nummod	3,minutes
R4354	T5227	T5224	pobj	minutes,for
R4355	T5228	T5227	prep	for,minutes
R4356	T5229	T5228	pobj	detection,for
R4357	T5230	T5220	punct	.,exposed
R4682	T5650	T5694	nsubjpass	Role,utilized
R4683	T5651	T5650	prep	of,Role
R4684	T5652	T5651	pobj	PKA,of
R4685	T5653	T5650	prep	on,Role
R4686	T5654	T5656	amod	LPS-induced,cell
R4687	T5655	T5656	compound	mononuclear,cell
R4688	T5656	T5653	pobj	cell,on
R4689	T5657	T5658	amod	TNF-α,production
R4690	T5658	T5660	dative	production,evaluate
R4691	T5659	T5660	aux	To,evaluate
R4692	T5660	T5650	advcl	evaluate,Role
R4693	T5661	T5662	det	the,role
R4694	T5662	T5660	dobj	role,evaluate
R4695	T5663	T5662	prep	of,role
R4696	T5664	T5663	pobj	PKA,of
R4697	T5665	T5662	prep	on,role
R4698	T5666	T5667	amod	TNF-α,production
R4699	T5667	T5665	pobj	production,on
R4700	T5668	T5667	prep	in,production
R4701	T5669	T5670	amod	mononuclear,cells
R4702	T5670	T5668	pobj	cells,in
R4703	T5671	T5670	cc	and,cells
R4704	T5672	T5673	poss	its,involvement
R4705	T5673	T5662	conj	involvement,role
R4706	T5674	T5673	prep	in,involvement
R4707	T5675	T5676	det	the,attenuation
R4708	T5676	T5674	pobj	attenuation,in
R4709	T5677	T5676	prep	of,attenuation
R4710	T5678	T5680	amod	LPS-induced,production
R4711	T5679	T5680	compound	TNF-α,production
R4712	T5680	T5677	pobj	production,of
R4713	T5681	T5694	ccomp	observed,utilized
R4714	T5682	T5681	prep	following,observed
R4715	T5683	T5684	compound	PTX,treatment
R4716	T5684	T5682	pobj	treatment,following
R4717	T5685	T5684	punct	",",treatment
R4718	T5686	T5694	nsubjpass	H89,utilized
R4719	T5687	T5686	punct	",",H89
R4720	T5688	T5691	det	a,inhibitor
R4721	T5689	T5691	amod	specific,inhibitor
R4722	T5690	T5691	compound	PKA,inhibitor
R4723	T5691	T5686	appos	inhibitor,H89
R4724	T5692	T5694	punct	",",utilized
R4725	T5693	T5694	auxpass	was,utilized
R4726	T5694	T5694	ROOT	utilized,utilized
R4727	T5695	T5694	punct	.,utilized
R4728	T5696	T5698	amod	Isolated,cells
R4729	T5697	T5698	compound	mononuclear,cells
R4730	T5698	T5700	nsubjpass	cells,incubated
R4731	T5699	T5700	auxpass	were,incubated
R4732	T5700	T5700	ROOT	incubated,incubated
R4733	T5701	T5700	prep	according,incubated
R4734	T5702	T5701	prep	to,according
R4735	T5703	T5705	det	the,groups
R4736	T5704	T5705	compound	treatment,groups
R4737	T5705	T5702	pobj	groups,to
R4738	T5706	T5705	acl	described,groups
R4739	T5707	T5706	advmod	above,described
R4740	T5708	T5706	prep	in,described
R4741	T5709	T5710	det	the,presence
R4742	T5710	T5708	pobj	presence,in
R4743	T5711	T5710	cc	and,presence
R4744	T5712	T5710	conj	absence,presence
R4745	T5713	T5712	prep	of,absence
R4746	T5714	T5713	pobj	pretreatment,of
R4747	T5715	T5706	prep	with,described
R4748	T5716	T5715	pobj	H89,with
R4749	T5717	T5719	punct	(,μM
R4750	T5718	T5719	nummod	10,μM
R4751	T5719	T5716	appos	μM,H89
R4752	T5720	T5701	punct	),according
R4753	T5721	T5700	prep	for,incubated
R4754	T5722	T5723	nummod	1,hour
R4755	T5723	T5721	pobj	hour,for
R4756	T5724	T5700	prep	at,incubated
R4757	T5725	T5724	pobj	37ºC,at
R4758	T5726	T5700	punct	.,incubated
R4759	T5727	T5728	det	The,dose
R4760	T5728	T5732	nsubjpass	dose,chosen
R4761	T5729	T5728	prep	of,dose
R4762	T5730	T5729	pobj	H89,of
R4763	T5731	T5732	auxpass	was,chosen
R4764	T5732	T5732	ROOT	chosen,chosen
R4765	T5733	T5732	prep	based,chosen
R4766	T5734	T5733	prep	on,based
R4767	T5735	T5736	amod	previous,work
R4768	T5736	T5734	pobj	work,on
R4769	T5737	T5738	nsubj	which,demonstrated
R4770	T5738	T5736	relcl	demonstrated,work
R4771	T5739	T5742	amod	specific,inhibition
R4772	T5740	T5739	cc	and,specific
R4773	T5741	T5739	conj	complete,specific
R4774	T5742	T5738	dobj	inhibition,demonstrated
R4775	T5743	T5742	prep	of,inhibition
R4776	T5744	T5743	pobj	PKA,of
R4777	T5745	T5738	prep	at,demonstrated
R4778	T5746	T5747	det	this,concentration
R4779	T5747	T5745	pobj	concentration,at
R4780	T5748	T5747	nummod	.10,concentration
R4781	T5749	T5755	mark	Since,is
R4782	T5750	T5751	det	the,activity
R4783	T5751	T5749	pobj	activity,Since
R4784	T5752	T5751	prep	of,activity
R4785	T5753	T5754	det	this,inhibitor
R4786	T5754	T5752	pobj	inhibitor,of
R4787	T5755	T5768	advcl	is,include
R4788	T5756	T5757	preconj	both,specific
R4789	T5757	T5755	acomp	specific,is
R4790	T5758	T5757	cc	and,specific
R4791	T5759	T5757	conj	consistent,specific
R4792	T5760	T5759	prep	at,consistent
R4793	T5761	T5763	det	this,dose
R4794	T5762	T5763	amod	specified,dose
R4795	T5763	T5760	pobj	dose,at
R4796	T5764	T5768	punct	",",include
R4797	T5765	T5768	nsubj	we,include
R4798	T5766	T5768	aux	did,include
R4799	T5767	T5768	neg	not,include
R4800	T5768	T5732	advcl	include,chosen
R4801	T5769	T5768	dobj	experiments,include
R4802	T5770	T5769	prep	with,experiments
R4803	T5771	T5773	amod	additional,inhibitors
R4804	T5772	T5773	compound	PKA,inhibitors
R4805	T5773	T5770	pobj	inhibitors,with
R4806	T5774	T5769	prep	with,experiments
R4807	T5775	T5776	det	this,set
R4808	T5776	T5774	pobj	set,with
R4809	T5777	T5776	prep	of,set
R4810	T5778	T5777	pobj	experiments,of
R4811	T5779	T5768	punct	.,include
R4812	T5780	T5782	det	The,concentration
R4813	T5781	T5782	amod	TNF-α,concentration
R4814	T5782	T5787	nsubjpass	concentration,measured
R4815	T5783	T5782	prep	in,concentration
R4816	T5784	T5785	det	the,supernatant
R4817	T5785	T5783	pobj	supernatant,in
R4818	T5786	T5787	auxpass	was,measured
R4819	T5787	T5787	ROOT	measured,measured
R4820	T5788	T5787	advmod	quantitatively,measured
R4821	T5789	T5787	agent	by,measured
R4822	T5790	T5789	pobj	ELISA,by
R4823	T5791	T5787	punct	.,measured
R4824	T5792	T5793	det	The,results
R4825	T5793	T5795	nsubjpass	results,expressed
R4826	T5794	T5795	auxpass	are,expressed
R4827	T5795	T5795	ROOT	expressed,expressed
R4828	T5796	T5795	prep	in,expressed
R4829	T5797	T5796	pobj	pg/mL,in
R4830	T5798	T5795	punct	.,expressed
R5035	T6072	T6073	compound	Statistical,Analysis
R5036	T6073	T6082	nsubjpass	Analysis,derived
R5037	T6074	T6076	det	The,results
R5038	T6075	T6076	amod	experimental,results
R5039	T6076	T6082	nsubjpass	results,derived
R5040	T6077	T6076	acl	obtained,results
R5041	T6078	T6077	prep	in,obtained
R5042	T6079	T6080	det	this,study
R5043	T6080	T6078	pobj	study,in
R5044	T6081	T6082	auxpass	were,derived
R5045	T6082	T6082	ROOT	derived,derived
R5046	T6083	T6082	prep	from,derived
R5047	T6084	T6086	nummod	four,experiments
R5048	T6085	T6086	amod	separate,experiments
R5049	T6086	T6083	pobj	experiments,from
R5050	T6087	T6086	prep	with,experiments
R5051	T6088	T6090	amod	healthy,donors
R5052	T6089	T6090	compound	volunteer,donors
R5053	T6090	T6087	pobj	donors,with
R5054	T6091	T6082	punct	.,derived
R5055	T6092	T6093	det	Each,assay
R5056	T6093	T6095	nsubjpass	assay,performed
R5057	T6094	T6095	auxpass	was,performed
R5058	T6095	T6095	ROOT	performed,performed
R5059	T6096	T6095	prep	in,performed
R5060	T6097	T6096	pobj	duplicate,in
R5061	T6098	T6097	cc	or,duplicate
R5062	T6099	T6097	conj	triplicate,duplicate
R5063	T6100	T6101	advmod	where,appropriate
R5064	T6101	T6099	advcl	appropriate,triplicate
R5065	T6102	T6095	punct	.,performed
R5066	T6103	T6105	nsubjpass	Data,presented
R5067	T6104	T6105	auxpass	is,presented
R5068	T6105	T6105	ROOT	presented,presented
R5069	T6106	T6105	prep	as,presented
R5070	T6107	T6110	det	the,SEM
R5071	T6108	T6110	amod	mean,SEM
R5072	T6109	T6110	compound	±,SEM
R5073	T6110	T6106	pobj	SEM,as
R5074	T6111	T6105	punct	.,presented
R5075	T6112	T6113	compound	Statistical,differences
R5076	T6113	T6117	nsubjpass	differences,determined
R5077	T6114	T6113	prep	between,differences
R5078	T6115	T6114	pobj	groups,between
R5079	T6116	T6117	auxpass	were,determined
R5080	T6117	T6117	ROOT	determined,determined
R5081	T6118	T6117	agent	by,determined
R5082	T6119	T6120	amod	one-way,analysis
R5083	T6120	T6118	pobj	analysis,by
R5084	T6121	T6120	prep	of,analysis
R5085	T6122	T6121	pobj	variance,of
R5086	T6123	T6124	punct	(,ANOVA
R5087	T6124	T6122	appos	ANOVA,variance
R5088	T6125	T6124	punct	),ANOVA
R5089	T6126	T6120	prep	with,analysis
R5090	T6127	T6129	det	a,correction
R5091	T6128	T6129	compound	Bonferroni,correction
R5092	T6129	T6126	pobj	correction,with
R5093	T6130	T6117	punct	.,determined
R5094	T6131	T6132	compound	Statistical,significance
R5095	T6132	T6134	nsubjpass	significance,defined
R5096	T6133	T6134	auxpass	was,defined
R5097	T6134	T6134	ROOT	defined,defined
R5098	T6135	T6134	prep	as,defined
R5099	T6136	T6135	pobj	P,as
R5100	T6137	T6136	nummod	<,P
R5101	T6138	T6136	nummod	0.05,P
R5102	T6139	T6134	punct	.,defined
R2160	T2733	T2732	pobj	Pierce,from

UBERON-AE

Id	Subject	Object	Predicate	Lexical cue
T2438	210-215	http://purl.obolibrary.org/obo/UBERON_0000178	denotes	blood
T2439	331-336	http://purl.obolibrary.org/obo/UBERON_0000025	denotes	tubes
T2440	1774-1778	http://purl.obolibrary.org/obo/UBERON_0012245	denotes	Silk
T3244	1820-1825	http://purl.obolibrary.org/obo/UBERON_0000178	denotes	Blood
T3245	1927-1932	http://purl.obolibrary.org/obo/UBERON_0000178	denotes	blood
T3246	2200-2205	http://purl.obolibrary.org/obo/UBERON_0000025	denotes	tubes
T3247	2322-2327	http://purl.obolibrary.org/obo/UBERON_0001977	denotes	serum
T4128	4780-4784	http://purl.obolibrary.org/obo/UBERON_0001913	denotes	milk
T4129	5671-5674	http://purl.obolibrary.org/obo/UBERON_0001460	denotes	arm
T4130	5830-5834	http://purl.obolibrary.org/obo/UBERON_0001460	denotes	arms

GO-BP

Id	Subject	Object	Predicate	Lexical cue
T3127	298-300	http://purl.obolibrary.org/obo/GO_0033968	denotes	CA
T3128	492-494	http://purl.obolibrary.org/obo/GO_0033968	denotes	CA
T3129	726-728	http://purl.obolibrary.org/obo/GO_0033968	denotes	CA
T3130	1296-1298	http://purl.obolibrary.org/obo/GO_0033968	denotes	CA
T3131	595-598	http://purl.obolibrary.org/obo/GO_0004691	denotes	PKA
T3884	3304-3320	http://purl.obolibrary.org/obo/GO_0032680	denotes	TNF-α production
T3885	3496-3526	http://purl.obolibrary.org/obo/GO_0032680	denotes	regulation in TNF-α production
T3886	3304-3320	http://purl.obolibrary.org/obo/GO_0032640	denotes	TNF-α production
T3887	3510-3526	http://purl.obolibrary.org/obo/GO_0032640	denotes	TNF-α production
T3888	3476-3486	http://purl.obolibrary.org/obo/GO_0065007	denotes	regulation
T3889	3496-3506	http://purl.obolibrary.org/obo/GO_0065007	denotes	regulation
T3890	3496-3515	http://purl.obolibrary.org/obo/GO_0042534	denotes	regulation in TNF-α
T3891	3496-3515	http://purl.obolibrary.org/obo/GO_1904467	denotes	regulation in TNF-α
T3892	3496-3526	http://purl.obolibrary.org/obo/GO_0032720	denotes	regulation in TNF-α production
T3893	3496-3526	http://purl.obolibrary.org/obo/GO_0032760	denotes	regulation in TNF-α production
T4080	3613-3629	http://purl.obolibrary.org/obo/GO_0032680	denotes	TNF-α production
T4081	3613-3629	http://purl.obolibrary.org/obo/GO_0032640	denotes	TNF-α production
T5960	7615-7618	http://purl.obolibrary.org/obo/GO_0004691	denotes	PKA
T5961	7692-7695	http://purl.obolibrary.org/obo/GO_0004691	denotes	PKA
T5962	7858-7861	http://purl.obolibrary.org/obo/GO_0004691	denotes	PKA
T5963	8165-8168	http://purl.obolibrary.org/obo/GO_0004691	denotes	PKA
T5964	8334-8337	http://purl.obolibrary.org/obo/GO_0004691	denotes	PKA
T5965	7651-7667	http://purl.obolibrary.org/obo/GO_0032680	denotes	TNF-α production
T5966	7699-7715	http://purl.obolibrary.org/obo/GO_0032680	denotes	TNF-α production
T5967	7791-7807	http://purl.obolibrary.org/obo/GO_0032680	denotes	TNF-α production
T5968	7651-7667	http://purl.obolibrary.org/obo/GO_0032640	denotes	TNF-α production
T5969	7699-7715	http://purl.obolibrary.org/obo/GO_0032640	denotes	TNF-α production
T5970	7791-7807	http://purl.obolibrary.org/obo/GO_0032640	denotes	TNF-α production

GO-MF

Id	Subject	Object	Predicate	Lexical cue
T3144	298-300	http://purl.obolibrary.org/obo/GO_0033968	denotes	CA
T3145	492-494	http://purl.obolibrary.org/obo/GO_0033968	denotes	CA
T3146	726-728	http://purl.obolibrary.org/obo/GO_0033968	denotes	CA
T3147	1296-1298	http://purl.obolibrary.org/obo/GO_0033968	denotes	CA
T3148	595-598	http://purl.obolibrary.org/obo/GO_0004691	denotes	PKA
T3149	883-893	http://purl.obolibrary.org/obo/GO_0003823	denotes	antibodies
T4827	4929-4937	http://purl.obolibrary.org/obo/GO_0003823	denotes	antibody
T4828	5022-5030	http://purl.obolibrary.org/obo/GO_0003823	denotes	antibody
T4829	5062-5070	http://purl.obolibrary.org/obo/GO_0003823	denotes	antibody
T4830	5246-5254	http://purl.obolibrary.org/obo/GO_0003823	denotes	antibody
T4831	5442-5450	http://purl.obolibrary.org/obo/GO_0003823	denotes	antibody
T5578	6248-6261	http://purl.obolibrary.org/obo/GO_0051059	denotes	NF-κB binding
T5579	6254-6261	http://purl.obolibrary.org/obo/GO_0005488	denotes	binding
T5580	6850-6857	http://purl.obolibrary.org/obo/GO_0005488	denotes	binding
T5581	6957-6964	http://purl.obolibrary.org/obo/GO_0005488	denotes	binding
T5971	7615-7618	http://purl.obolibrary.org/obo/GO_0004691	denotes	PKA
T5972	7692-7695	http://purl.obolibrary.org/obo/GO_0004691	denotes	PKA
T5973	7858-7861	http://purl.obolibrary.org/obo/GO_0004691	denotes	PKA
T5974	8165-8168	http://purl.obolibrary.org/obo/GO_0004691	denotes	PKA
T5975	8334-8337	http://purl.obolibrary.org/obo/GO_0004691	denotes	PKA

GO-CC

Id	Subject	Object	Predicate	Lexical cue
T3153	914-918	http://purl.obolibrary.org/obo/GO_0005623	denotes	Cell
T3154	1211-1220	http://purl.obolibrary.org/obo/GO_0016020	denotes	membranes
T3155	1609-1618	http://purl.obolibrary.org/obo/GO_0016020	denotes	membranes
T3901	1838-1842	http://purl.obolibrary.org/obo/GO_0005623	denotes	Cell
T3902	2069-2073	http://purl.obolibrary.org/obo/GO_0005623	denotes	Cell
T3903	2449-2453	http://purl.obolibrary.org/obo/GO_0005623	denotes	Cell
T3904	2549-2554	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T3905	2741-2746	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T3906	2777-2782	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T4086	3645-3650	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T4087	3733-3738	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T4832	4035-4039	http://purl.obolibrary.org/obo/GO_0005623	denotes	cell
T4833	4471-4475	http://purl.obolibrary.org/obo/GO_0005623	denotes	Cell
T4834	4734-4743	http://purl.obolibrary.org/obo/GO_0016020	denotes	membranes
T4835	4749-4758	http://purl.obolibrary.org/obo/GO_0016020	denotes	membranes
T4836	5125-5134	http://purl.obolibrary.org/obo/GO_0016020	denotes	membranes
T4837	5369-5377	http://purl.obolibrary.org/obo/GO_0016020	denotes	membrane
T4838	4929-4937	http://purl.obolibrary.org/obo/GO_0019815	denotes	antibody
T4839	5022-5030	http://purl.obolibrary.org/obo/GO_0019815	denotes	antibody
T4840	5062-5070	http://purl.obolibrary.org/obo/GO_0019815	denotes	antibody
T4841	5246-5254	http://purl.obolibrary.org/obo/GO_0019815	denotes	antibody
T4842	5442-5450	http://purl.obolibrary.org/obo/GO_0019815	denotes	antibody
T4843	4929-4937	http://purl.obolibrary.org/obo/GO_0042571	denotes	antibody
T4844	5022-5030	http://purl.obolibrary.org/obo/GO_0042571	denotes	antibody
T4845	5062-5070	http://purl.obolibrary.org/obo/GO_0042571	denotes	antibody
T4846	5246-5254	http://purl.obolibrary.org/obo/GO_0042571	denotes	antibody
T4847	5442-5450	http://purl.obolibrary.org/obo/GO_0042571	denotes	antibody
T5582	7279-7287	http://purl.obolibrary.org/obo/GO_0016020	denotes	membrane
T5583	7538-7547	http://purl.obolibrary.org/obo/GO_0016020	denotes	membranes
T5978	7858-7861	http://purl.obolibrary.org/obo/GO_0005952	denotes	PKA
T5979	8165-8168	http://purl.obolibrary.org/obo/GO_0005952	denotes	PKA
T5980	8334-8337	http://purl.obolibrary.org/obo/GO_0005952	denotes	PKA
T5981	7646-7650	http://purl.obolibrary.org/obo/GO_0005623	denotes	cell
T5982	7731-7736	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T5983	7908-7913	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T5976	7615-7618	http://purl.obolibrary.org/obo/GO_0005952	denotes	PKA
T5977	7692-7695	http://purl.obolibrary.org/obo/GO_0005952	denotes	PKA
T3150	595-598	http://purl.obolibrary.org/obo/GO_0005952	denotes	PKA
T3151	883-893	http://purl.obolibrary.org/obo/GO_0019815	denotes	antibodies
T3152	883-893	http://purl.obolibrary.org/obo/GO_0042571	denotes	antibodies

sentences

Id	Subject	Object	Predicate	Lexical cue
T2454	303-391	Sentence	denotes	1.5-mL Eppendorf centrifuge tubes were purchased from Fisher Scientific (Pittsburg, PA).
T2455	392-496	Sentence	denotes	Hank’s balanced salt solution (HBSS) and RPMI 1640 were obtained from Irvine Scientific (Santa Ana, CA).
T2456	497-590	Sentence	denotes	LPS from Escherichia coli serotype 0111:B4 and PTX were purchased from Sigma (St. Louis, MO).
T2457	591-730	Sentence	denotes	The PKA inhibitor, N-[2-((p-bromocinnamyl)amino)ethyl]-5-isoguinolinesulfonamide-2HCl] (H89), was purchased from Calbiochem (La Jolla, CA).
T2458	731-1040	Sentence	denotes	Antibodies for phosphorylated CREB (serine 133), phosphorylated I-κBα (serines 32 and 36), phosphorylated NF-κB p65 subunit (serine 276), and secondary antibodies were purchased from Cell Signaling (Beverly, MA). Dextran T500 and Percoll were received from GE Healthcare/Amersham Biosciences (Piscataway, NJ).
T2459	1041-1300	Sentence	denotes	The enzyme-linked immunosorbent assay (ELISA) for TNF-α was obtained from Quantikine R&D Systems (Minneapolis, MN). Tris-glycine and DNA retardation gels, nitrocellulose membranes, and western blot running buffers were obtained from Invitrogen (Carlsbad, CA).
T2460	1301-1518	Sentence	denotes	NE-PER® nuclear and cytoplasmic extraction reagents, the BCA protein reagent kit, the Supersignal West Pico Chemiluminescent Kit, and the LightShift Chemiluminescent EMSA Kit were purchased from Pierce (Rockland, IL).
T2461	1519-1602	Sentence	denotes	CREB and NF-κB DNA oligonucleotide probes were synthesized by IDT (Coralville, IA).
T2462	1603-1681	Sentence	denotes	Nylon membranes were purchased from Roche Applied Sciences (Indianapolis, IN).
T2463	1682-1801	Sentence	denotes	Western blot band quantification was performed with the UN-SCAN-IT Gel Digitizing software (Silk Scientific, Orem, UT).
T3255	1803-1868	Sentence	denotes	Human Peripheral Blood Mononuclear Cell Isolation and Stimulation
T3256	1869-1966	Sentence	denotes	Human mononuclear cells were isolated from the peripheral blood of four healthy human volunteers.
T3257	1967-2172	Sentence	denotes	The sample size was chosen from our previously published work, which demonstrated reliable results.10 Cell isolation and all subsequent experiments were conducted under sterile and pyrogen-free conditions.
T3258	2173-2307	Sentence	denotes	Blood collected in heparin tubes was incubated with Dextran T500 and the red cells were sedimented for 40 minutes at room temperature.
T3259	2308-2448	Sentence	denotes	The resultant serum was then washed with HBSS and separated by Percoll gradient centrifugation according to the manufacturer’s instructions.
T3260	2449-2539	Sentence	denotes	Cell viability was assessed by trypan blue dye exclusion, with purity of greater than 95%.
T3261	2540-2664	Sentence	denotes	Isolated cells were resuspended in RPMI 1640 supplemented with 10% FBS and 5 mM HEPES at a concentration of 1 x 107cells/mL.
T3262	2665-2767	Sentence	denotes	Each subsequent experiment listed below was conducted on samples of 5 x 106 cells per treatment group.
T3263	2768-2955	Sentence	denotes	Isolated cells were stimulated with either HBSS as a negative control, LPS (1 μg/mL), PTX (20 mM), or concomitant LPS / PTX, at the above-mentioned concentrations, for 30 minutes at 37ºC.
T3264	2956-3080	Sentence	denotes	Cells were placed on ice for 10 minutes to stop the reaction and the samples were then stored at −70°C for further analysis.
T3265	3081-3321	Sentence	denotes	The stimulation times and concentrations of LPS and PTX utilized in this study were determined in previous pilot studies done in our laboratory, which examined the effects of increasing concentrations of PTX on LPS-induced TNF-α production.
T3266	3322-3582	Sentence	denotes	LPS at a concentration of 1 μg/mL and PTX at a concentration of 20 mM were the minimum concentrations necessary to produce a reproducible and reliable up-regulation and down-regulation in TNF-α production, respectively, in quantitative assays (data not shown).
T3968	3584-3650	Sentence	denotes	Effect of PTX on LPS-induced TNF-α production by mononuclear cells
T3969	3651-3852	Sentence	denotes	The concentration of TNF-α was quantitatively determined by ELISA in the media of cells exposed to the treatments described above after stimulation for up to a maximum of 18 hours, at 3-hour intervals.
T3970	3853-3888	Sentence	denotes	The results are expressed in pg/mL.
T4138	3890-3946	Sentence	denotes	SDS-PAGE and Immunoblotting (I-κBα, NF-κB p65, and CREB)
T4139	3947-4047	Sentence	denotes	Following stimulation, PBMCs were washed with ice-cold PBS and centrifuged to collect a cell pellet.
T4140	4048-4145	Sentence	denotes	The pellet was resuspended in ice-cold SDS sample buffer supplemented with 100 mM dithiothreitol.
T4141	4146-4335	Sentence	denotes	Cytoplasmic and nuclear extracts were isolated with NE-PER nuclear and cytoplasmic extraction reagents with 1x Halt Protease Inhibitor Cocktail according to the manufacturer’s instructions.
T4142	4336-4470	Sentence	denotes	Protein concentrations were determined with the BCA protein reagent kit for each sample according to a standardized curve for albumin.
T4143	4471-4558	Sentence	denotes	Cell lysates were collected by boiling the samples in a 100ºC water bath for 5 minutes.
T4144	4559-4744	Sentence	denotes	Ten μg of protein per sample was separated by SDS-polyacrylamide gel electrophoresis through 8–16% tris-glycine polyacrylamide gradient gels and transferred to nitrocellulose membranes.
T4145	4745-4866	Sentence	denotes	The membranes were blocked with 5% milk in Tris-buffered saline/Tween 20 (Fischer Scientific, Pittsburgh, PA) for 1 hour.
T4146	4867-5120	Sentence	denotes	Cytoplasmic extracts were incubated with phosphorylated I-κBα antibody (1:200), while nuclear extracts were incubated with either phosphorylated NF-κB p65 antibody (1:500) or phosphorylated CREB antibody (1:500) overnight at 4°C in separate experiments.
T4147	5121-5338	Sentence	denotes	The membranes were washed with Tris-buffered saline/Tween 20 and incubated for 1 hour at room temperature with the secondary antibody, horseradish peroxidase-linked anti-rabbit IgG diluted 1:2000 in blocking solution.
T4148	5339-5497	Sentence	denotes	After repeated washing of the membrane, the Supersignal West Pico Chemiluminescent Kit was applied for antibody detection per the manufacturer’s instructions.
T4149	5498-5564	Sentence	denotes	Western blot data is presented as a percentage of LPS stimulation.
T4150	5565-5761	Sentence	denotes	The percentage of LPS stimulation was calculated by dividing the mean band pixel total for each treatment arm divided by the mean pixel total of samples stimulated with LPS and multiplying by 100.
T4151	5762-5879	Sentence	denotes	Thus, LPS stimulation is reported as 100% and each of the treatment arms is reported as a percent of LPS stimulation.
T4895	5881-5939	Sentence	denotes	NF-κB and CREB Electrophoretic Mobility Shift Assay (EMSA)
T4896	5940-6041	Sentence	denotes	The non-radioactive LightShift Chemiluminescent EMSA Kit was used to detect DNA-protein interactions.
T4897	6042-6281	Sentence	denotes	The NF-κB 3′ biotin end-labeled DNA oligonucleotide used as a probe for the EMSA was a 42-bp double stranded construct (5′-TTGTTACAA-GGGGACTTTCCGCTGGGGACTTTCCAGGGAGGC – 3′) containing two tandemly repeated NF-κB binding sites (underlined).
T4898	6282-6421	Sentence	denotes	Specificity was determined by a competition assay with the addition of 200 molar excess of unlabeled double stranded NF-κB oligonucleotide.
T4899	6422-6629	Sentence	denotes	The CREB 3′ biotin end-labeled DNA oligonucleotide used as a probe for the EMSA was a 23-bp double stranded construct (5′-TTT TCG AGC TCTGACGTCAGA-GC – 3′) containing the CRE consensus sequence (underlined).
T4900	6630-6768	Sentence	denotes	Specificity was determined by a competition assay with the addition of 200 molar excess of unlabeled double stranded CREB oligonucleotide.
T4901	6769-6846	Sentence	denotes	Nuclear extracts (10 μg) were incubated with 5 nM NF-κB or CREB probe (NF-kB:
T4902	6847-6953	Sentence	denotes	1x binding buffer, 50 mM KCl, 1 mM EDTA, 1 mM DTT, 0.1% NP40, 10% glycerol, and 50 ng/μl poly dI-dC, CREB:
T4903	6954-7175	Sentence	denotes	1x binding buffer, 20 mM Tris, pH 7.5, 50 mM KCl, 1 mM EDTA, 1 mM DTT, 0.10% NP40, 6% glycerol, 0.1 mg/mL BSA and 50 ng/μl poly dI-dC) and were then electrophoresed through a 6% DNA retardation gel at 100V for 90 minutes.
T4904	7176-7387	Sentence	denotes	The gels were electrophoretically transferred at 380mA for 1 hour on ice to a positively charged nylon membrane and immediately cross-linked for 15 minutes with a UV transilluminator equipped with a 312 nm bulb.
T4905	7388-7527	Sentence	denotes	Streptavidin-horseradish peroxidase conjugate and the LightShift Chemiluminescent Substrate were used to detect the biotin end-labeled DNA.
T4906	7528-7605	Sentence	denotes	The nylon membranes were exposed to x-ray film for 1–3 minutes for detection.
T5643	7607-7667	Sentence	denotes	Role of PKA on LPS-induced mononuclear cell TNF-α production
T5644	7668-7886	Sentence	denotes	To evaluate the role of PKA on TNF-α production in mononuclear cells and its involvement in the attenuation of LPS-induced TNF-α production observed following PTX treatment, H89, a specific PKA inhibitor, was utilized.
T5645	7887-8059	Sentence	denotes	Isolated mononuclear cells were incubated according to the treatment groups described above in the presence and absence of pretreatment with H89 (10 μM) for 1 hour at 37ºC.
T5646	8060-8378	Sentence	denotes	The dose of H89 was chosen based on previous work which demonstrated specific and complete inhibition of PKA at this concentration.10 Since the activity of this inhibitor is both specific and consistent at this specified dose, we did not include experiments with additional PKA inhibitors with this set of experiments.
T5647	8379-8459	Sentence	denotes	The TNF-α concentration in the supernatant was measured quantitatively by ELISA.
T5648	8460-8495	Sentence	denotes	The results are expressed in pg/mL.
T6066	8497-8517	Sentence	denotes	Statistical Analysis
T6067	8518-8640	Sentence	denotes	The experimental results obtained in this study were derived from four separate experiments with healthy volunteer donors.
T6068	8641-8711	Sentence	denotes	Each assay was performed in duplicate or triplicate where appropriate.
T6069	8712-8748	Sentence	denotes	Data is presented as the mean ± SEM.
T6070	8749-8873	Sentence	denotes	Statistical differences between groups were determined by one-way analysis of variance (ANOVA) with a Bonferroni correction.
T6071	8874-8923	Sentence	denotes	Statistical significance was defined as P < 0.05.
T36	0-21	Sentence	denotes	MATERIALS AND METHODS
T37	22-123	Sentence	denotes	This study was approved by the Human Research Protections Program and the Institutional Review Board.
T38	124-225	Sentence	denotes	Written consent to participate in the study was obtained from all volunteers prior to blood donation.
T39	226-302	Sentence	denotes	Heparinized Vacutainers were purchased from Becton Dickinson (San Jose, CA).
T40	303-391	Sentence	denotes	1.5-mL Eppendorf centrifuge tubes were purchased from Fisher Scientific (Pittsburg, PA).
T41	392-496	Sentence	denotes	Hank’s balanced salt solution (HBSS) and RPMI 1640 were obtained from Irvine Scientific (Santa Ana, CA).
T42	497-590	Sentence	denotes	LPS from Escherichia coli serotype 0111:B4 and PTX were purchased from Sigma (St. Louis, MO).
T43	591-730	Sentence	denotes	The PKA inhibitor, N-[2-((p-bromocinnamyl)amino)ethyl]-5-isoguinolinesulfonamide-2HCl] (H89), was purchased from Calbiochem (La Jolla, CA).
T44	731-943	Sentence	denotes	Antibodies for phosphorylated CREB (serine 133), phosphorylated I-κBα (serines 32 and 36), phosphorylated NF-κB p65 subunit (serine 276), and secondary antibodies were purchased from Cell Signaling (Beverly, MA).
T45	944-1040	Sentence	denotes	Dextran T500 and Percoll were received from GE Healthcare/Amersham Biosciences (Piscataway, NJ).
T46	1041-1156	Sentence	denotes	The enzyme-linked immunosorbent assay (ELISA) for TNF-α was obtained from Quantikine R&D Systems (Minneapolis, MN).
T47	1157-1300	Sentence	denotes	Tris-glycine and DNA retardation gels, nitrocellulose membranes, and western blot running buffers were obtained from Invitrogen (Carlsbad, CA).
T48	1301-1518	Sentence	denotes	NE-PER® nuclear and cytoplasmic extraction reagents, the BCA protein reagent kit, the Supersignal West Pico Chemiluminescent Kit, and the LightShift Chemiluminescent EMSA Kit were purchased from Pierce (Rockland, IL).
T49	1519-1602	Sentence	denotes	CREB and NF-κB DNA oligonucleotide probes were synthesized by IDT (Coralville, IA).
T50	1603-1681	Sentence	denotes	Nylon membranes were purchased from Roche Applied Sciences (Indianapolis, IN).
T51	1682-1801	Sentence	denotes	Western blot band quantification was performed with the UN-SCAN-IT Gel Digitizing software (Silk Scientific, Orem, UT).
T52	1803-1868	Sentence	denotes	Human Peripheral Blood Mononuclear Cell Isolation and Stimulation
T53	1869-1966	Sentence	denotes	Human mononuclear cells were isolated from the peripheral blood of four healthy human volunteers.
T54	1967-2172	Sentence	denotes	The sample size was chosen from our previously published work, which demonstrated reliable results.10 Cell isolation and all subsequent experiments were conducted under sterile and pyrogen-free conditions.
T55	2173-2307	Sentence	denotes	Blood collected in heparin tubes was incubated with Dextran T500 and the red cells were sedimented for 40 minutes at room temperature.
T56	2308-2448	Sentence	denotes	The resultant serum was then washed with HBSS and separated by Percoll gradient centrifugation according to the manufacturer’s instructions.
T57	2449-2539	Sentence	denotes	Cell viability was assessed by trypan blue dye exclusion, with purity of greater than 95%.
T58	2540-2664	Sentence	denotes	Isolated cells were resuspended in RPMI 1640 supplemented with 10% FBS and 5 mM HEPES at a concentration of 1 x 107cells/mL.
T59	2665-2767	Sentence	denotes	Each subsequent experiment listed below was conducted on samples of 5 x 106 cells per treatment group.
T60	2768-2955	Sentence	denotes	Isolated cells were stimulated with either HBSS as a negative control, LPS (1 μg/mL), PTX (20 mM), or concomitant LPS / PTX, at the above-mentioned concentrations, for 30 minutes at 37ºC.
T61	2956-3080	Sentence	denotes	Cells were placed on ice for 10 minutes to stop the reaction and the samples were then stored at −70°C for further analysis.
T62	3081-3321	Sentence	denotes	The stimulation times and concentrations of LPS and PTX utilized in this study were determined in previous pilot studies done in our laboratory, which examined the effects of increasing concentrations of PTX on LPS-induced TNF-α production.
T63	3322-3582	Sentence	denotes	LPS at a concentration of 1 μg/mL and PTX at a concentration of 20 mM were the minimum concentrations necessary to produce a reproducible and reliable up-regulation and down-regulation in TNF-α production, respectively, in quantitative assays (data not shown).
T64	3584-3650	Sentence	denotes	Effect of PTX on LPS-induced TNF-α production by mononuclear cells
T65	3651-3852	Sentence	denotes	The concentration of TNF-α was quantitatively determined by ELISA in the media of cells exposed to the treatments described above after stimulation for up to a maximum of 18 hours, at 3-hour intervals.
T66	3853-3888	Sentence	denotes	The results are expressed in pg/mL.
T67	3890-3946	Sentence	denotes	SDS-PAGE and Immunoblotting (I-κBα, NF-κB p65, and CREB)
T68	3947-4047	Sentence	denotes	Following stimulation, PBMCs were washed with ice-cold PBS and centrifuged to collect a cell pellet.
T69	4048-4145	Sentence	denotes	The pellet was resuspended in ice-cold SDS sample buffer supplemented with 100 mM dithiothreitol.
T70	4146-4335	Sentence	denotes	Cytoplasmic and nuclear extracts were isolated with NE-PER nuclear and cytoplasmic extraction reagents with 1x Halt Protease Inhibitor Cocktail according to the manufacturer’s instructions.
T71	4336-4470	Sentence	denotes	Protein concentrations were determined with the BCA protein reagent kit for each sample according to a standardized curve for albumin.
T72	4471-4558	Sentence	denotes	Cell lysates were collected by boiling the samples in a 100ºC water bath for 5 minutes.
T73	4559-4744	Sentence	denotes	Ten μg of protein per sample was separated by SDS-polyacrylamide gel electrophoresis through 8–16% tris-glycine polyacrylamide gradient gels and transferred to nitrocellulose membranes.
T74	4745-4866	Sentence	denotes	The membranes were blocked with 5% milk in Tris-buffered saline/Tween 20 (Fischer Scientific, Pittsburgh, PA) for 1 hour.
T75	4867-5120	Sentence	denotes	Cytoplasmic extracts were incubated with phosphorylated I-κBα antibody (1:200), while nuclear extracts were incubated with either phosphorylated NF-κB p65 antibody (1:500) or phosphorylated CREB antibody (1:500) overnight at 4°C in separate experiments.
T76	5121-5338	Sentence	denotes	The membranes were washed with Tris-buffered saline/Tween 20 and incubated for 1 hour at room temperature with the secondary antibody, horseradish peroxidase-linked anti-rabbit IgG diluted 1:2000 in blocking solution.
T77	5339-5497	Sentence	denotes	After repeated washing of the membrane, the Supersignal West Pico Chemiluminescent Kit was applied for antibody detection per the manufacturer’s instructions.
T78	5498-5564	Sentence	denotes	Western blot data is presented as a percentage of LPS stimulation.
T79	5565-5761	Sentence	denotes	The percentage of LPS stimulation was calculated by dividing the mean band pixel total for each treatment arm divided by the mean pixel total of samples stimulated with LPS and multiplying by 100.
T80	5762-5879	Sentence	denotes	Thus, LPS stimulation is reported as 100% and each of the treatment arms is reported as a percent of LPS stimulation.
T81	5881-5939	Sentence	denotes	NF-κB and CREB Electrophoretic Mobility Shift Assay (EMSA)
T82	5940-6041	Sentence	denotes	The non-radioactive LightShift Chemiluminescent EMSA Kit was used to detect DNA-protein interactions.
T83	6042-6281	Sentence	denotes	The NF-κB 3′ biotin end-labeled DNA oligonucleotide used as a probe for the EMSA was a 42-bp double stranded construct (5′-TTGTTACAA-GGGGACTTTCCGCTGGGGACTTTCCAGGGAGGC – 3′) containing two tandemly repeated NF-κB binding sites (underlined).
T84	6282-6421	Sentence	denotes	Specificity was determined by a competition assay with the addition of 200 molar excess of unlabeled double stranded NF-κB oligonucleotide.
T85	6422-6629	Sentence	denotes	The CREB 3′ biotin end-labeled DNA oligonucleotide used as a probe for the EMSA was a 23-bp double stranded construct (5′-TTT TCG AGC TCTGACGTCAGA-GC – 3′) containing the CRE consensus sequence (underlined).
T86	6630-6768	Sentence	denotes	Specificity was determined by a competition assay with the addition of 200 molar excess of unlabeled double stranded CREB oligonucleotide.
T87	6769-6846	Sentence	denotes	Nuclear extracts (10 μg) were incubated with 5 nM NF-κB or CREB probe (NF-kB:
T88	6847-6953	Sentence	denotes	1x binding buffer, 50 mM KCl, 1 mM EDTA, 1 mM DTT, 0.1% NP40, 10% glycerol, and 50 ng/μl poly dI-dC, CREB:
T89	6954-7175	Sentence	denotes	1x binding buffer, 20 mM Tris, pH 7.5, 50 mM KCl, 1 mM EDTA, 1 mM DTT, 0.10% NP40, 6% glycerol, 0.1 mg/mL BSA and 50 ng/μl poly dI-dC) and were then electrophoresed through a 6% DNA retardation gel at 100V for 90 minutes.
T90	7176-7387	Sentence	denotes	The gels were electrophoretically transferred at 380mA for 1 hour on ice to a positively charged nylon membrane and immediately cross-linked for 15 minutes with a UV transilluminator equipped with a 312 nm bulb.
T91	7388-7527	Sentence	denotes	Streptavidin-horseradish peroxidase conjugate and the LightShift Chemiluminescent Substrate were used to detect the biotin end-labeled DNA.
T92	7528-7605	Sentence	denotes	The nylon membranes were exposed to x-ray film for 1–3 minutes for detection.
T93	7607-7667	Sentence	denotes	Role of PKA on LPS-induced mononuclear cell TNF-α production
T94	7668-7886	Sentence	denotes	To evaluate the role of PKA on TNF-α production in mononuclear cells and its involvement in the attenuation of LPS-induced TNF-α production observed following PTX treatment, H89, a specific PKA inhibitor, was utilized.
T95	7887-8059	Sentence	denotes	Isolated mononuclear cells were incubated according to the treatment groups described above in the presence and absence of pretreatment with H89 (10 μM) for 1 hour at 37ºC.
T96	8060-8378	Sentence	denotes	The dose of H89 was chosen based on previous work which demonstrated specific and complete inhibition of PKA at this concentration.10 Since the activity of this inhibitor is both specific and consistent at this specified dose, we did not include experiments with additional PKA inhibitors with this set of experiments.
T97	8379-8459	Sentence	denotes	The TNF-α concentration in the supernatant was measured quantitatively by ELISA.
T98	8460-8495	Sentence	denotes	The results are expressed in pg/mL.
T99	8497-8517	Sentence	denotes	Statistical Analysis
T100	8518-8640	Sentence	denotes	The experimental results obtained in this study were derived from four separate experiments with healthy volunteer donors.
T101	8641-8711	Sentence	denotes	Each assay was performed in duplicate or triplicate where appropriate.
T102	8712-8748	Sentence	denotes	Data is presented as the mean ± SEM.
T103	8749-8873	Sentence	denotes	Statistical differences between groups were determined by one-way analysis of variance (ANOVA) with a Bonferroni correction.
T104	8874-8923	Sentence	denotes	Statistical significance was defined as P < 0.05.

events-check-again

Id	Subject	Object	Predicate	Lexical cue
T3205	746-760	Phosphorylation	denotes	phosphorylated
T3206	761-765	Protein	denotes	CREB
T3207	767-777	Entity	denotes	serine 133
T3208	780-794	Phosphorylation	denotes	phosphorylated
T3209	780-794	Phosphorylation	denotes	phosphorylated
T3210	795-800	Protein	denotes	I-κBα
T3211	802-812	Entity	denotes	serines 32
T3212	817-819	Entity	denotes	36
T3213	822-836	Phosphorylation	denotes	phosphorylated
T3214	843-846	Protein	denotes	p65
T3215	856-866	Entity	denotes	serine 276
T3216	1091-1096	Protein	denotes	TNF-α
T3941	3296-3303	Positive_regulation	denotes	induced
T3942	3304-3309	Protein	denotes	TNF-α
T3943	3310-3320	Gene_expression	denotes	production
T3944	3473-3486	Positive_regulation	denotes	up-regulation
T3945	3491-3506	Negative_regulation	denotes	down-regulation
T3946	3510-3515	Protein	denotes	TNF-α
T3947	3516-3526	Gene_expression	denotes	production
T4106	3605-3612	Positive_regulation	denotes	induced
T4107	3613-3618	Protein	denotes	TNF-α
T4108	3619-3629	Gene_expression	denotes	production
T4109	3672-3677	Protein	denotes	TNF-α
T4881	3919-3924	Protein	denotes	I-κBα
T4882	3932-3935	Protein	denotes	p65
T4883	3941-3945	Protein	denotes	CREB
T4884	4908-4922	Phosphorylation	denotes	phosphorylated
T4885	4923-4928	Protein	denotes	I-κBα
T4886	4997-5011	Phosphorylation	denotes	phosphorylated
T4887	5018-5021	Protein	denotes	p65
T6040	7607-7611	Regulation	denotes	Role
T6041	7626-7633	Positive_regulation	denotes	induced
T6042	7651-7656	Protein	denotes	TNF-α
T6043	7657-7667	Gene_expression	denotes	production
T6044	7684-7688	Regulation	denotes	role
T6045	7699-7704	Protein	denotes	TNF-α
T6046	7705-7715	Gene_expression	denotes	production
T6047	7745-7756	Regulation	denotes	involvement
T6048	7764-7775	Negative_regulation	denotes	attenuation
T6049	7783-7790	Positive_regulation	denotes	induced
T6050	7791-7796	Protein	denotes	TNF-α
T6051	7797-7807	Gene_expression	denotes	production
T6052	8383-8388	Protein	denotes	TNF-α
R2569	T3207	T3205	themeOf	serine 133,phosphorylated
R2570	T3207	T3206	partOf	serine 133,CREB
R2571	T3211	T3209	themeOf	serines 32,phosphorylated
R2572	T3211	T3210	partOf	serines 32,I-κBα
R2573	T3212	T3208	themeOf	36,phosphorylated
R2574	T3212	T3210	partOf	36,I-κBα
R2575	T3215	T3213	themeOf	serine 276,phosphorylated
R2576	T3215	T3214	partOf	serine 276,p65
R3234	T3942	T3943	themeOf	TNF-α,production
R3235	T3943	T3941	themeOf	production,induced
R3236	T3946	T3947	themeOf	TNF-α,production
R3237	T3947	T3944	themeOf	production,up-regulation
R3238	T3947	T3945	themeOf	production,down-regulation
R3363	T4107	T4108	themeOf	TNF-α,production
R3364	T4108	T4106	themeOf	production,induced
R4030	T4885	T4884	themeOf	I-κBα,phosphorylated
R4031	T4887	T4886	themeOf	p65,phosphorylated
R5017	T6041	T6040	themeOf	induced,Role
R5018	T6042	T6043	themeOf	TNF-α,production
R5019	T6043	T6041	themeOf	production,induced
R5020	T6045	T6046	themeOf	TNF-α,production
R5021	T6046	T6044	themeOf	production,role
R5022	T6048	T6047	themeOf	attenuation,involvement
R5023	T6049	T6048	themeOf	induced,attenuation
R5024	T6050	T6051	themeOf	TNF-α,production
R5025	T6051	T6049	themeOf	production,induced

bionlp-st-ge-2016-reference-tees

Id	Subject	Object	Predicate	Lexical cue
T3156	544-547	Protein	denotes	PTX
T3157	595-598	Protein	denotes	PKA
T3158	761-765	Protein	denotes	CREB
T3159	780-800	Protein	denotes	phosphorylated I-κBα
T3160	837-842	Protein	denotes	NF-κB
T3161	843-854	Protein	denotes	p65 subunit
T3162	746-760	Phosphorylation	denotes	phosphorylated
T3163	822-836	Phosphorylation	denotes	phosphorylated
T3164	822-836	Phosphorylation	denotes	phosphorylated
T3165	1091-1096	Protein	denotes	TNF-α
T3166	1358-1369	Protein	denotes	BCA protein
T3167	1519-1523	Protein	denotes	CREB
T3168	1528-1553	Protein	denotes	NF-κB DNA oligonucleotide
T3169	1566-1577	Gene_expression	denotes	synthesized
T3170	1566-1577	Gene_expression	denotes	synthesized
T3907	2575-2579	Protein	denotes	RPMI
T3908	2888-2891	Protein	denotes	PTX
T3909	3285-3288	Protein	denotes	PTX
T3910	3304-3309	Protein	denotes	TNF-α
T3911	3310-3320	Gene_expression	denotes	production
T3912	3245-3252	Regulation	denotes	effects
T3913	3360-3363	Protein	denotes	PTX
T3914	3510-3515	Protein	denotes	TNF-α
T3915	3516-3526	Gene_expression	denotes	production
T3916	3473-3486	Positive_regulation	denotes	up-regulation
T3917	3491-3506	Negative_regulation	denotes	down-regulation
T3918	3491-3506	Negative_regulation	denotes	down-regulation
T3919	3473-3486	Positive_regulation	denotes	up-regulation
T3920	3473-3486	Positive_regulation	denotes	up-regulation
T4088	3594-3597	Protein	denotes	PTX
T4089	3613-3618	Protein	denotes	TNF-α
T4090	3619-3629	Gene_expression	denotes	production
T4091	3584-3590	Regulation	denotes	Effect
T4092	3601-3612	Positive_regulation	denotes	LPS-induced
T4093	3672-3677	Protein	denotes	TNF-α
T4848	3926-3935	Protein	denotes	NF-κB p65
T4849	3941-3945	Protein	denotes	CREB
T4850	4254-4280	Protein	denotes	1x Halt Protease Inhibitor
T4851	4384-4395	Protein	denotes	BCA protein
T4852	4462-4469	Protein	denotes	albumin
T4853	4923-4937	Protein	denotes	I-κBα antibody
T4854	5012-5030	Protein	denotes	NF-κB p65 antibody
T4855	5057-5061	Protein	denotes	CREB
T4856	4908-4922	Phosphorylation	denotes	phosphorylated
T4857	5042-5056	Phosphorylation	denotes	phosphorylated
T4858	5042-5056	Phosphorylation	denotes	phosphorylated
T4859	5256-5301	Protein	denotes	horseradish peroxidase-linked anti-rabbit IgG
T5584	5881-5886	Protein	denotes	NF-κB
T5585	5891-5895	Protein	denotes	CREB
T5586	6046-6053	Protein	denotes	NF-κB 3
T5587	6055-6061	Protein	denotes	biotin
T5588	6248-6253	Protein	denotes	NF-κB
T5589	6399-6420	Protein	denotes	NF-κB oligonucleotide
T5590	6426-6440	Protein	denotes	CREB 3′ biotin
T5591	6747-6751	Protein	denotes	CREB
T5592	6819-6824	Protein	denotes	NF-κB
T5593	6828-6832	Protein	denotes	CREB
T5594	6941-6946	Protein	denotes	dI-dC
T5595	6948-6956	Protein	denotes	CREB: 1x
T5596	7388-7423	Protein	denotes	Streptavidin-horseradish peroxidase
T5597	7504-7510	Protein	denotes	biotin
T5984	7615-7618	Protein	denotes	PKA
T5985	7651-7656	Protein	denotes	TNF-α
T5986	7657-7667	Gene_expression	denotes	production
T5987	7607-7611	Regulation	denotes	Role
T5988	7622-7633	Positive_regulation	denotes	LPS-induced
T5989	7692-7695	Protein	denotes	PKA
T5990	7699-7704	Protein	denotes	TNF-α
T5991	7791-7796	Protein	denotes	TNF-α
T5992	7858-7861	Protein	denotes	PKA
T5993	7705-7715	Gene_expression	denotes	production
T5994	7797-7807	Gene_expression	denotes	production
T5995	7862-7871	Negative_regulation	denotes	inhibitor
T5996	7684-7688	Regulation	denotes	role
T5997	7764-7775	Negative_regulation	denotes	attenuation
T5998	7745-7756	Regulation	denotes	involvement
T5999	8165-8168	Protein	denotes	PKA
T6000	8334-8337	Protein	denotes	PKA
T6001	8151-8161	Negative_regulation	denotes	inhibition
T6002	8338-8348	Negative_regulation	denotes	inhibitors
T6003	8383-8388	Protein	denotes	TNF-α
R2541	T3158	T3162	themeOf	CREB,phosphorylated
R2542	T3160	T3163	themeOf	NF-κB,phosphorylated
R2543	T3161	T3164	themeOf	p65 subunit,phosphorylated
R2544	T3167	T3169	themeOf	CREB,synthesized
R2545	T3168	T3170	themeOf	NF-κB DNA oligonucleotide,synthesized
R3211	T3909	T3912	causeOf	PTX,effects
R3212	T3910	T3911	themeOf	TNF-α,production
R3213	T3911	T3912	themeOf	production,effects
R3214	T3914	T3915	themeOf	TNF-α,production
R3215	T3915	T3916	themeOf	production,up-regulation
R3216	T3915	T3918	themeOf	production,down-regulation
R3217	T3916	T3917	themeOf	up-regulation,down-regulation
R3218	T3917	T3919	themeOf	down-regulation,up-regulation
R3219	T3918	T3920	themeOf	down-regulation,up-regulation
R3353	T4088	T4091	causeOf	PTX,Effect
R3354	T4089	T4090	themeOf	TNF-α,production
R3355	T4090	T4091	themeOf	production,Effect
R3356	T4090	T4092	themeOf	production,LPS-induced
R4021	T4853	T4856	themeOf	I-κBα antibody,phosphorylated
R4022	T4854	T4857	themeOf	NF-κB p65 antibody,phosphorylated
R4023	T4855	T4858	themeOf	CREB,phosphorylated
R4980	T5984	T5987	causeOf	PKA,Role
R4981	T5985	T5986	themeOf	TNF-α,production
R4982	T5986	T5987	themeOf	production,Role
R4983	T5986	T5988	themeOf	production,LPS-induced
R4984	T5989	T5996	causeOf	PKA,role
R4985	T5990	T5993	themeOf	TNF-α,production
R4986	T5991	T5994	themeOf	TNF-α,production
R4987	T5992	T5995	themeOf	PKA,inhibitor
R4988	T5993	T5996	themeOf	production,role
R4989	T5994	T5997	themeOf	production,attenuation
R4990	T5997	T5998	themeOf	attenuation,involvement
R4991	T5999	T6001	themeOf	PKA,inhibition
R4992	T6000	T6002	themeOf	PKA,inhibitors

bionlp-st-ge-2016-uniprot

Id	Subject	Object	Predicate	Lexical cue
T3100	761-765	Q8TEY5	denotes	CREB
T3101	761-765	Q02930	denotes	CREB
T3102	761-765	P18848	denotes	CREB
T3103	761-765	P16220	denotes	CREB
T3104	761-765	O43889	denotes	CREB
T3105	761-765	P15336	denotes	CREB
T3106	843-846	Q04206	denotes	p65
T3107	843-846	P21579	denotes	p65
T3108	1091-1094	P01375	denotes	TNF
T3109	1091-1096	P01375	denotes	TNF-α
T3110	1519-1523	P15336	denotes	CREB
T3111	1519-1523	P18848	denotes	CREB
T3112	1519-1523	P16220	denotes	CREB
T3113	1519-1523	Q02930	denotes	CREB
T3114	1519-1523	Q8TEY5	denotes	CREB
T3115	1519-1523	O43889	denotes	CREB
T3868	3304-3307	P01375	denotes	TNF
T3869	3304-3309	P01375	denotes	TNF-α
T3870	3510-3513	P01375	denotes	TNF
T3871	3510-3515	P01375	denotes	TNF-α
T4073	3613-3616	P01375	denotes	TNF
T4074	3613-3618	P01375	denotes	TNF-α
T4075	3672-3675	P01375	denotes	TNF
T4076	3672-3677	P01375	denotes	TNF-α
T4790	3932-3935	Q04206	denotes	p65
T4791	3932-3935	P21579	denotes	p65
T4792	3941-3945	P15336	denotes	CREB
T4793	3941-3945	P16220	denotes	CREB
T4794	3941-3945	P18848	denotes	CREB
T4795	3941-3945	O43889	denotes	CREB
T4796	3941-3945	Q02930	denotes	CREB
T4797	3941-3945	Q8TEY5	denotes	CREB
T4798	5018-5021	Q04206	denotes	p65
T4799	5018-5021	P21579	denotes	p65
T4800	5057-5061	P15336	denotes	CREB
T4801	5057-5061	P18848	denotes	CREB
T4802	5057-5061	P16220	denotes	CREB
T4803	5057-5061	Q02930	denotes	CREB
T4804	5057-5061	Q8TEY5	denotes	CREB
T4805	5057-5061	O43889	denotes	CREB
T5533	5891-5895	O43889	denotes	CREB
T5534	5891-5895	Q8TEY5	denotes	CREB
T5535	5891-5895	Q02930	denotes	CREB
T5536	5891-5895	P18848	denotes	CREB
T5537	5891-5895	P16220	denotes	CREB
T5538	5891-5895	P15336	denotes	CREB
T5539	6426-6430	O43889	denotes	CREB
T5540	6426-6430	Q8TEY5	denotes	CREB
T5541	6426-6430	Q02930	denotes	CREB
T5542	6426-6430	P18848	denotes	CREB
T5543	6426-6430	P15336	denotes	CREB
T5544	6426-6430	P16220	denotes	CREB
T5545	6426-6432	O43889	denotes	CREB 3
T5546	6593-6596	P06956	denotes	CRE
T5547	6747-6751	Q02930	denotes	CREB
T5548	6747-6751	P18848	denotes	CREB
T5549	6747-6751	Q8TEY5	denotes	CREB
T5550	6747-6751	P15336	denotes	CREB
T5551	6747-6751	P16220	denotes	CREB
T5552	6747-6751	O43889	denotes	CREB
T5553	6828-6832	Q02930	denotes	CREB
T5554	6828-6832	P18848	denotes	CREB
T5555	6828-6832	P16220	denotes	CREB
T5556	6828-6832	P15336	denotes	CREB
T5557	6828-6832	O43889	denotes	CREB
T5558	6828-6832	Q8TEY5	denotes	CREB
T5559	6948-6952	Q8TEY5	denotes	CREB
T5560	6948-6952	P18848	denotes	CREB
T5561	6948-6952	P16220	denotes	CREB
T5562	6948-6952	P15336	denotes	CREB
T5563	6948-6952	Q02930	denotes	CREB
T5564	6948-6952	O43889	denotes	CREB
T5565	6985-6987	P0A7Z4	denotes	pH
T5942	7651-7654	P01375	denotes	TNF
T5943	7651-7656	P01375	denotes	TNF-α
T5944	7699-7702	P01375	denotes	TNF
T5945	7699-7704	P01375	denotes	TNF-α
T5946	7791-7794	P01375	denotes	TNF
T5947	7791-7796	P01375	denotes	TNF-α
T5948	8383-8386	P01375	denotes	TNF
T5949	8383-8388	P01375	denotes	TNF-α

test2

Id	Subject	Object	Predicate	Lexical cue
T2427	746-760	Phosphorylation	denotes	phosphorylated
T2428	761-765	Protein	denotes	CREB
T2429	767-777	Entity	denotes	serine 133
T2430	780-794	Phosphorylation	denotes	phosphorylated
T2431	795-800	Protein	denotes	I-κBα
T2432	802-812	Entity	denotes	serines 32
T2433	817-819	Entity	denotes	36
T2434	822-836	Phosphorylation	denotes	phosphorylated
T2435	843-846	Protein	denotes	p65
T2436	863-866	Entity	denotes	276
T2437	1091-1096	Protein	denotes	TNF-α
T3236	3245-3252	Regulation	denotes	effects
T3237	3296-3303	Positive_regulation	denotes	induced
T3238	3304-3309	Protein	denotes	TNF-α
T3239	3310-3320	Gene_expression	denotes	production
T3240	3473-3486	Positive_regulation	denotes	up-regulation
T3241	3491-3506	Negative_regulation	denotes	down-regulation
T3242	3510-3515	Protein	denotes	TNF-α
T3243	3516-3526	Gene_expression	denotes	production
T3959	3584-3590	Regulation	denotes	Effect
T3960	3605-3612	Positive_regulation	denotes	induced
T3961	3613-3618	Protein	denotes	TNF-α
T3962	3619-3629	Gene_expression	denotes	production
T3963	3672-3677	Protein	denotes	TNF-α
T4121	3919-3924	Protein	denotes	I-κBα
T4122	3932-3935	Protein	denotes	p65
T4123	3941-3945	Protein	denotes	CREB
T4124	4908-4922	Phosphorylation	denotes	phosphorylated
T4125	4923-4928	Protein	denotes	I-κBα
T4126	4997-5011	Phosphorylation	denotes	phosphorylated
T4127	5018-5021	Protein	denotes	p65
T5611	7626-7633	Positive_regulation	denotes	induced
T5612	7651-7656	Protein	denotes	TNF-α
T5613	7657-7667	Gene_expression	denotes	production
T5614	7684-7688	Regulation	denotes	role
T5615	7699-7704	Protein	denotes	TNF-α
T5616	7705-7715	Gene_expression	denotes	production
T5617	7783-7790	Positive_regulation	denotes	induced
T5618	7791-7796	Protein	denotes	TNF-α
T5619	7797-7807	Gene_expression	denotes	production
T5620	7817-7826	Positive_regulation	denotes	following
T5621	8383-8388	Protein	denotes	TNF-α
R1877	T2429	T2428	partOf	serine 133,CREB
R1878	T2429	T2427	themeOf	serine 133,phosphorylated
R1879	T2432	T2430	themeOf	serines 32,phosphorylated
R1880	T2432	T2431	partOf	serines 32,I-κBα
R1881	T2433	T2431	partOf	36,I-κBα
R1882	T2433	T2430	themeOf	36,phosphorylated
R2590	T3238	T3239	themeOf	TNF-α,production
R2591	T3239	T3237	themeOf	production,induced
R2592	T3242	T3243	themeOf	TNF-α,production
R2593	T3243	T3241	themeOf	production,down-regulation
R2594	T3243	T3240	themeOf	production,up-regulation
R3246	T3961	T3962	themeOf	TNF-α,production
R3247	T3962	T3960	themeOf	production,induced
R3369	T4125	T4124	themeOf	I-κBα,phosphorylated
R3370	T4127	T4126	themeOf	p65,phosphorylated
R4660	T5612	T5613	themeOf	TNF-α,production
R4661	T5613	T5611	themeOf	production,induced
R4662	T5615	T5616	themeOf	TNF-α,production
R4663	T5617	T5620	themeOf	induced,following
R4664	T5618	T5619	themeOf	TNF-α,production
R4665	T5619	T5620	themeOf	production,following
R4666	T5619	T5617	themeOf	production,induced

PMC:2664230 / 4921-13845 JSONTXT

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PMC:2664230 / 4921-13845 JSON TXT