GRSN Graphical Visualization Quality control graphs are plotted for each sample as it is normalized. For each sample, three M vs. A scatter plots are generated: The first is pre GRSN, and compares the given sample to the virtual reference sample (a pseudo sample with each transcript set to the trimmed mean value for that transcript from all samples in a dataset). The second shows the GRiS transcripts only, both before and after adjustment, and includes the lowess smoothed calibration curve. The third shows the given sample, after adjustment, compared to the virtual reference sample.