Luciferase assay
MEF5-/- and BT-549 cells were transfected with the Spi2.1-containing luciferase reporter plasmid. Forty-eight hours post transfection, lysates were prepared and luciferase activity was measured [24]. The luciferase values (arbitrary units), as measured by a Berthold Luminometer (Berthold, Oak Ridge, TN, USA), were normalized to total protein.