Supporting Information
Figure S1  Partial Sequences of Genes rpsL, fadE5, and phoP in M. tuberculosis H37Ra and H37Ra Knock-In Strains
Arrows indicate the nucleotide variation at the corresponding SNP site resulting from complementation with the wild-type gene from M. tuberculosis H37Rv.
(212 KB PDF) Click here for additional data file.   Figure S2  Restriction and Hybridization Profiles of M. tuberculosis H37Ra and H37Ra::phoP 
SpeI-digested, pulsed field gel electrophoresis (PFGE) profiles of M. tuberculosis H37Ra and H37Ra::phoP (left panel), and after hybridization with a radiolabeled phoP probe (right panel).
(155 KB PDF) Click here for additional data file.   Figure S3  Colony Morphology of Different M. tuberculosis H37 Strains
(35 KB PDF) Click here for additional data file.   Figure S4  Sizes of Spleens from SCID Mice
SCID mice were intravenously infected with different M. tuberculosis H37 strains (106 bacteria) for 3 wk (A) or 4 wk (B).
(59 KB PDF) Click here for additional data file.   Table S1  Identity and Genomic Position of Mutations in the M. tuberculosis H37Ra Strain Used in This Study
(48 KB DOC) Click here for additional data file.   Table S2  Primers and Probes Used for Quantitative RT-PCR
(22 KB DOC) Click here for additional data file.