Yeast Strains
Strain JJ160 (ydj1::HIS3) (Johnson and Craig 2000) was crossed to the isogenic strain WY26 (sis1::LEU2) (Yan and Craig 1999) to yield JJ1146 (MAT  a trp1-1 ura3-1 leu2-3,112 his3-11,15 ade2-1 can1-100 met2-Δ1 lys2-Δ2 ydj1::HIS3 sis1::LEU2)/pYW17(SIS1-YCp50). Strain JJ261 (MATα ade1-100 Δtrp1 ura3-52 leu2-3,112 ydj1::HIS3 Δ hem1) was constructed by crossing the nonisogenic strains MH1 (MAT  a, ura3-52 leu2-112 his4-519 ade1-100 Δ hem1) (Haldi and Guarente 1989) and the ydj1::HIS3 disruption strain JJ257 (Johnson and Craig 2000).