Phylogenetic analyses.
TRIP13 orthologs were identified by BLASTP searches of Genbank and other sources providing gene models such as Ensembl. The selected orthologs can be found in Table S1. Amino acid alignments were done with Clustal W, using the default settings with and without removing the regions outside of the AAA-ATPase central domain. The trees were constructed by using the neighbor-joining method with Poisson correction. The reliability of internal branches was assessed by using 500 bootstrap replicates, and sites with gaps were ignored in this analysis. Neighbor-joining searches were conducted by using the computer program MEGA3 [66].