A peptide corresponding to amino acids 25–40 of TRIP13, VLQRSGSTAKKEDIK, was conjugated to KLH and used to immunize chickens (done by Sigma Genosys, http://www.sigmaaldrich.com). Polyclonal IgY was isolated from eggs with the Eggcellent Chicken IgY Purification kit (Pierce, http://www.piercenet.com). IgY antibodies were then affinity purified using the immunizing synthetic peptide.