Trip13 was amplified from samples of Clontech's Mouse Multiple Tissue cDNA Panel I (http://www.clontech.com), using the following primers: 5′-GCACCATTGCACTTCACATC-3′ (TRP3-6F) and 5′-TGACCATCAGACTGTCGAGC-3′ (TRP3-6R). These primers correspond to exons 3 and 6, respectively, and amplify a 330-bp cDNA product. The cDNAs in this panel are equalized to allow quantitative analysis by RT-PCR.