Consistent with the presence of rare (<1%) Trip13Gt/Gt pachytene spermatocytes devoid of persistent DNA repair markers, and testis histology showing some degree of postmeiotic progression (Figure 3G), we observed both diplotene nuclei that lacked autosomal RAD51/DMC1 and γH2AX (Figure S3A–S3D), and also metaphase I spreads with 20 bivalents (Figure S3E–S3F). Since Trip13Gt may not be a complete null, these diplotene and metaphase I spermatocytes might arise by virtue of having sufficient wild-type TRIP13.