Histology of Mutant Gonads
All are hematoxylin/eosin-stained paraffin sections. Testes are from 6-wk-old males, except as indicated below.
(A) Wild-type 25-d-old ovary.
(B) Trip13Gt /Gt 25-d-old ovary, showing dysgenesis from an absence of oocytes.
(C) Trip13Gt/+ 2-d-old control ovary. Arrows point to oocytes in newly forming follicles.
(D) Trip13Gt/Gt 2-d-old ovary, dysgenic due to lack of oocytes. Magnification is the same as its littermate in “C.”
(E) Wild-type testis.
(F) Trip13Gt/Gt testis with uniform pachytene arrest.
(G) Trip13Gt/Gt 3-mo-old testis with some postmeiotic spermatids (arrows).
(H) Spo11−/- testis. A tubule with spermatocytes at leptotene/zygotene transition is labeled ZP, and tubules with apoptotic spermatocytes are marked with an asterisk. The specimen was taken from a littermate of that in (I).
(I) Spo11 −/− Trip13Gt/Gt testis. Labeling is the same as in (H). The inset contains a tubule with leptotene-zygotene spermatocytes.
(J) Mei1 −/− Trip13Gt/+ testis. The specimen was taken from a littermate of that in (K).
(K) Mei1 −/− Trip13Gt/Gt testis.
(L) Rec8Mei8/Rec8Mei8 Trip13Gt/+ testis. The Rec8Mei8 allele was described [39]. The specimen was taken from a littermate of that in (M).
(M) Rec8Mei8/Rec8Mei8 Trip13Gt/Gt testis.
(N) Dmc1 −/− Trip13Gt/Gt testis.
(O) Spo11 −/− Trip13Gt/+ 25-d-old ovary. The specimen was taken from a littermate of that in (P).
(P) Spo11 −/− Trip13Gt/Gt 25-d-old ovary.
(Q) Mei1 −/− Trip13Gt/+ 25-d-old ovary. The specimen was taken from a littermate of that in (R).
(R) Mei1 −/− Trip13Gt/Gt 25-d-old ovary.
(S) Rec8Mei8/Rec8Mei8 Trip13Gt/+ 25-d-old ovary. The specimen was taken from a littermate of that in (T).
(T) Rec8Mei8/Rec8Mei8 Trip13Gt/Gt 25-d-old ovary.