Table 4  Validation of gene expression changes in the E18.5 Pygo1/Pygo2 null kidneys normalized to E18.5 wild-type kidneys.
Gene symbol  Microarray fold change  QPCR fold change (SD)  Validation
Aldh1a7  - 2.2  1.1 (0.11)  No
Col8a1  2.1  2.9 (0.78)  Yes
Csrp1  - 7.1  1.2 (0.02)  No
Klk5  2.8  5.8 (0.74)  Yes
Pygo2  - 3.9  - 3.7 (0.04)  Yes
Picalm  3.1  2.2 (0.24)  Yes
Ren2  2.2  6.1 (0.36)  Yes
Tia1  6.6 and 2.3  Amplification not detected in wild-type samples  Yes