PMC:1858683 / 21717-22276
Annnotations
craft-sa-dev
{"project":"craft-sa-dev","denotations":[{"id":"T26928","span":{"begin":61,"end":62},"obj":"HYPH"},{"id":"T26927","span":{"begin":62,"end":67},"obj":"NN"},{"id":"T26926","span":{"begin":56,"end":61},"obj":"NN"},{"id":"T26925","span":{"begin":53,"end":55},"obj":"IN"},{"id":"T26924","span":{"begin":42,"end":52},"obj":"NN"},{"id":"T26923","span":{"begin":33,"end":41},"obj":"NN"},{"id":"T26922","span":{"begin":28,"end":29},"obj":"HYPH"},{"id":"T26921","span":{"begin":29,"end":32},"obj":"NN"},{"id":"T26920","span":{"begin":25,"end":28},"obj":"NN"},{"id":"T26919","span":{"begin":22,"end":24},"obj":"IN"},{"id":"T26918","span":{"begin":13,"end":21},"obj":"NN"},{"id":"T26917","span":{"begin":0,"end":12},"obj":"JJ"},{"id":"T27007","span":{"begin":386,"end":387},"obj":"."},{"id":"T27006","span":{"begin":385,"end":386},"obj":"NN"},{"id":"T27005","span":{"begin":383,"end":385},"obj":"CD"},{"id":"T27004","span":{"begin":377,"end":382},"obj":"IN"},{"id":"T27003","span":{"begin":374,"end":376},"obj":"IN"},{"id":"T27002","span":{"begin":362,"end":365},"obj":"VBD"},{"id":"T27001","span":{"begin":351,"end":361},"obj":"NN"},{"id":"T27000","span":{"begin":342,"end":350},"obj":"NN"},{"id":"T26999","span":{"begin":340,"end":342},"obj":","},{"id":"T26998","span":{"begin":333,"end":340},"obj":"RB"},{"id":"T26997","span":{"begin":331,"end":333},"obj":","},{"id":"T26996","span":{"begin":313,"end":323},"obj":"JJ"},{"id":"T26995","span":{"begin":307,"end":312},"obj":"NN"},{"id":"T26994","span":{"begin":305,"end":307},"obj":","},{"id":"T26993","span":{"begin":292,"end":305},"obj":"NN"},{"id":"T26992","span":{"begin":286,"end":291},"obj":"NN"},{"id":"T26991","span":{"begin":324,"end":331},"obj":"NNS"},{"id":"T26990","span":{"begin":282,"end":285},"obj":"DT"},{"id":"T26989","span":{"begin":366,"end":373},"obj":"VBN"},{"id":"T26988","span":{"begin":279,"end":281},"obj":"IN"},{"id":"T26987","span":{"begin":278,"end":387},"obj":"sentence"},{"id":"T26986","span":{"begin":277,"end":278},"obj":"."},{"id":"T26985","span":{"begin":267,"end":277},"obj":"NN"},{"id":"T26984","span":{"begin":262,"end":263},"obj":"HYPH"},{"id":"T26983","span":{"begin":263,"end":266},"obj":"NN"},{"id":"T26982","span":{"begin":259,"end":262},"obj":"NN"},{"id":"T26981","span":{"begin":256,"end":258},"obj":"IN"},{"id":"T26980","span":{"begin":249,"end":255},"obj":"NNS"},{"id":"T26979","span":{"begin":241,"end":248},"obj":"JJ"},{"id":"T26978","span":{"begin":230,"end":233},"obj":"RB"},{"id":"T26977","span":{"begin":234,"end":240},"obj":"VBD"},{"id":"T26976","span":{"begin":220,"end":221},"obj":"SYM"},{"id":"T26975","span":{"begin":219,"end":220},"obj":"HYPH"},{"id":"T26974","span":{"begin":218,"end":219},"obj":"SYM"},{"id":"T26973","span":{"begin":213,"end":218},"obj":"NN"},{"id":"T26972","span":{"begin":212,"end":213},"obj":"HYPH"},{"id":"T26971","span":{"begin":211,"end":212},"obj":"SYM"},{"id":"T26970","span":{"begin":210,"end":211},"obj":"HYPH"},{"id":"T26969","span":{"begin":209,"end":210},"obj":"SYM"},{"id":"T26968","span":{"begin":204,"end":209},"obj":"NN"},{"id":"T26967","span":{"begin":200,"end":203},"obj":"CC"},{"id":"T26966","span":{"begin":198,"end":199},"obj":"SYM"},{"id":"T26965","span":{"begin":197,"end":198},"obj":"HYPH"},{"id":"T26964","span":{"begin":196,"end":197},"obj":"SYM"},{"id":"T26963","span":{"begin":191,"end":196},"obj":"NN"},{"id":"T26962","span":{"begin":190,"end":191},"obj":"HYPH"},{"id":"T26961","span":{"begin":189,"end":190},"obj":"SYM"},{"id":"T26960","span":{"begin":188,"end":189},"obj":"HYPH"},{"id":"T26959","span":{"begin":187,"end":188},"obj":"SYM"},{"id":"T26958","span":{"begin":182,"end":187},"obj":"NN"},{"id":"T26957","span":{"begin":180,"end":182},"obj":","},{"id":"T26956","span":{"begin":179,"end":180},"obj":"SYM"},{"id":"T26955","span":{"begin":178,"end":179},"obj":"HYPH"},{"id":"T26954","span":{"begin":177,"end":178},"obj":"SYM"},{"id":"T26953","span":{"begin":172,"end":177},"obj":"NN"},{"id":"T26952","span":{"begin":171,"end":172},"obj":"HYPH"},{"id":"T26951","span":{"begin":170,"end":171},"obj":"SYM"},{"id":"T26950","span":{"begin":169,"end":170},"obj":"HYPH"},{"id":"T26949","span":{"begin":168,"end":169},"obj":"SYM"},{"id":"T26948","span":{"begin":222,"end":229},"obj":"NNS"},{"id":"T26947","span":{"begin":163,"end":168},"obj":"NN"},{"id":"T26946","span":{"begin":162,"end":278},"obj":"sentence"},{"id":"T26945","span":{"begin":161,"end":162},"obj":"."},{"id":"T26944","span":{"begin":153,"end":161},"obj":"NN"},{"id":"T26943","span":{"begin":147,"end":152},"obj":"NNP"},{"id":"T26942","span":{"begin":144,"end":146},"obj":"IN"},{"id":"T26941","span":{"begin":129,"end":132},"obj":"VBD"},{"id":"T26940","span":{"begin":133,"end":143},"obj":"VBN"},{"id":"T26939","span":{"begin":114,"end":115},"obj":"HYPH"},{"id":"T26938","span":{"begin":110,"end":114},"obj":"NN"},{"id":"T26937","span":{"begin":115,"end":128},"obj":"NN"},{"id":"T26936","span":{"begin":100,"end":101},"obj":"HYPH"},{"id":"T26935","span":{"begin":101,"end":109},"obj":"JJ"},{"id":"T26934","span":{"begin":91,"end":100},"obj":"NN"},{"id":"T26933","span":{"begin":90,"end":162},"obj":"sentence"},{"id":"T26932","span":{"begin":89,"end":90},"obj":"."},{"id":"T26931","span":{"begin":74,"end":80},"obj":"NN"},{"id":"T26930","span":{"begin":68,"end":73},"obj":"NN"},{"id":"T26929","span":{"begin":81,"end":89},"obj":"NNS"},{"id":"T27042","span":{"begin":558,"end":559},"obj":"."},{"id":"T27041","span":{"begin":548,"end":553},"obj":"RBS"},{"id":"T27040","span":{"begin":554,"end":558},"obj":"CD"},{"id":"T27039","span":{"begin":545,"end":547},"obj":"RB"},{"id":"T27038","span":{"begin":542,"end":544},"obj":"IN"},{"id":"T27037","span":{"begin":530,"end":536},"obj":"NN"},{"id":"T27036","span":{"begin":537,"end":541},"obj":"NN"},{"id":"T27035","span":{"begin":528,"end":529},"obj":"DT"},{"id":"T27034","span":{"begin":519,"end":527},"obj":"VBD"},{"id":"T27033","span":{"begin":500,"end":512},"obj":"JJ"},{"id":"T27032","span":{"begin":513,"end":518},"obj":"NN"},{"id":"T27031","span":{"begin":495,"end":499},"obj":"DT"},{"id":"T27030","span":{"begin":494,"end":559},"obj":"sentence"},{"id":"T27029","span":{"begin":493,"end":494},"obj":"."},{"id":"T27028","span":{"begin":480,"end":485},"obj":"NN"},{"id":"T27027","span":{"begin":486,"end":493},"obj":"NNS"},{"id":"T27026","span":{"begin":474,"end":475},"obj":"HYPH"},{"id":"T27025","span":{"begin":475,"end":479},"obj":"NN"},{"id":"T27024","span":{"begin":470,"end":474},"obj":"JJ"},{"id":"T27023","span":{"begin":462,"end":469},"obj":"IN"},{"id":"T27022","span":{"begin":459,"end":461},"obj":"CC"},{"id":"T27021","span":{"begin":454,"end":458},"obj":"IN"},{"id":"T27020","span":{"begin":452,"end":454},"obj":","},{"id":"T27019","span":{"begin":449,"end":452},"obj":"JJ"},{"id":"T27018","span":{"begin":439,"end":448},"obj":"RB"},{"id":"T27017","span":{"begin":424,"end":434},"obj":"NN"},{"id":"T27016","span":{"begin":419,"end":420},"obj":"HYPH"},{"id":"T27015","span":{"begin":420,"end":423},"obj":"NN"},{"id":"T27014","span":{"begin":416,"end":419},"obj":"NN"},{"id":"T27013","span":{"begin":408,"end":415},"obj":"NNS"},{"id":"T27012","span":{"begin":397,"end":407},"obj":"JJ"},{"id":"T27011","span":{"begin":391,"end":396},"obj":"NN"},{"id":"T27010","span":{"begin":435,"end":438},"obj":"VBD"},{"id":"T27009","span":{"begin":388,"end":390},"obj":"IN"},{"id":"T27008","span":{"begin":387,"end":494},"obj":"sentence"}],"relations":[{"id":"R7770","pred":"amod","subj":"T26917","obj":"T26918"},{"id":"R7771","pred":"prep","subj":"T26919","obj":"T26918"},{"id":"R7772","pred":"compound","subj":"T26920","obj":"T26921"},{"id":"R7773","pred":"compound","subj":"T26921","obj":"T26923"},{"id":"R7774","pred":"punct","subj":"T26922","obj":"T26921"},{"id":"R7775","pred":"compound","subj":"T26923","obj":"T26924"},{"id":"R7776","pred":"pobj","subj":"T26924","obj":"T26919"},{"id":"R7777","pred":"prep","subj":"T26925","obj":"T26924"},{"id":"R7778","pred":"compound","subj":"T26926","obj":"T26927"},{"id":"R7779","pred":"compound","subj":"T26927","obj":"T26929"},{"id":"R7780","pred":"punct","subj":"T26928","obj":"T26927"},{"id":"R7781","pred":"pobj","subj":"T26929","obj":"T26925"},{"id":"R7782","pred":"compound","subj":"T26930","obj":"T26929"},{"id":"R7783","pred":"compound","subj":"T26931","obj":"T26929"},{"id":"R7784","pred":"punct","subj":"T26932","obj":"T26918"},{"id":"R7785","pred":"npadvmod","subj":"T26934","obj":"T26935"},{"id":"R7786","pred":"amod","subj":"T26935","obj":"T26937"},{"id":"R7787","pred":"punct","subj":"T26936","obj":"T26935"},{"id":"R7788","pred":"nsubjpass","subj":"T26937","obj":"T26940"},{"id":"R7789","pred":"compound","subj":"T26938","obj":"T26937"},{"id":"R7790","pred":"punct","subj":"T26939","obj":"T26937"},{"id":"R7791","pred":"auxpass","subj":"T26941","obj":"T26940"},{"id":"R7792","pred":"prep","subj":"T26942","obj":"T26940"},{"id":"R7793","pred":"compound","subj":"T26943","obj":"T26944"},{"id":"R7794","pred":"pobj","subj":"T26944","obj":"T26942"},{"id":"R7795","pred":"punct","subj":"T26945","obj":"T26940"},{"id":"R7796","pred":"nmod","subj":"T26947","obj":"T26948"},{"id":"R7797","pred":"nsubj","subj":"T26948","obj":"T26977"},{"id":"R7798","pred":"punct","subj":"T26949","obj":"T26947"},{"id":"R7799","pred":"punct","subj":"T26950","obj":"T26947"},{"id":"R7800","pred":"punct","subj":"T26951","obj":"T26947"},{"id":"R7801","pred":"punct","subj":"T26952","obj":"T26947"},{"id":"R7802","pred":"appos","subj":"T26953","obj":"T26947"},{"id":"R7803","pred":"punct","subj":"T26954","obj":"T26953"},{"id":"R7804","pred":"punct","subj":"T26955","obj":"T26953"},{"id":"R7805","pred":"punct","subj":"T26956","obj":"T26953"},{"id":"R7806","pred":"punct","subj":"T26957","obj":"T26947"},{"id":"R7807","pred":"conj","subj":"T26958","obj":"T26947"},{"id":"R7808","pred":"punct","subj":"T26959","obj":"T26958"},{"id":"R7809","pred":"punct","subj":"T26960","obj":"T26958"},{"id":"R7810","pred":"punct","subj":"T26961","obj":"T26958"},{"id":"R7811","pred":"punct","subj":"T26962","obj":"T26958"},{"id":"R7812","pred":"appos","subj":"T26963","obj":"T26958"},{"id":"R7813","pred":"punct","subj":"T26964","obj":"T26963"},{"id":"R7814","pred":"punct","subj":"T26965","obj":"T26963"},{"id":"R7815","pred":"punct","subj":"T26966","obj":"T26963"},{"id":"R7816","pred":"cc","subj":"T26967","obj":"T26958"},{"id":"R7817","pred":"conj","subj":"T26968","obj":"T26958"},{"id":"R7818","pred":"punct","subj":"T26969","obj":"T26968"},{"id":"R7819","pred":"punct","subj":"T26970","obj":"T26968"},{"id":"R7820","pred":"punct","subj":"T26971","obj":"T26968"},{"id":"R7821","pred":"punct","subj":"T26972","obj":"T26968"},{"id":"R7822","pred":"appos","subj":"T26973","obj":"T26968"},{"id":"R7823","pred":"punct","subj":"T26974","obj":"T26973"},{"id":"R7824","pred":"punct","subj":"T26975","obj":"T26973"},{"id":"R7825","pred":"punct","subj":"T26976","obj":"T26973"},{"id":"R7826","pred":"appos","subj":"T26978","obj":"T26948"},{"id":"R7827","pred":"amod","subj":"T26979","obj":"T26980"},{"id":"R7828","pred":"dobj","subj":"T26980","obj":"T26977"},{"id":"R7829","pred":"prep","subj":"T26981","obj":"T26980"},{"id":"R7830","pred":"compound","subj":"T26982","obj":"T26983"},{"id":"R7831","pred":"compound","subj":"T26983","obj":"T26985"},{"id":"R7832","pred":"punct","subj":"T26984","obj":"T26983"},{"id":"R7833","pred":"pobj","subj":"T26985","obj":"T26981"},{"id":"R7834","pred":"punct","subj":"T26986","obj":"T26977"},{"id":"R7835","pred":"prep","subj":"T26988","obj":"T26989"},{"id":"R7836","pred":"det","subj":"T26990","obj":"T26991"},{"id":"R7837","pred":"pobj","subj":"T26991","obj":"T26988"},{"id":"R7838","pred":"nmod","subj":"T26992","obj":"T26993"},{"id":"R7839","pred":"nmod","subj":"T26993","obj":"T26991"},{"id":"R7840","pred":"punct","subj":"T26994","obj":"T26991"},{"id":"R7841","pred":"npadvmod","subj":"T26995","obj":"T26996"},{"id":"R7842","pred":"amod","subj":"T26996","obj":"T26991"},{"id":"R7843","pred":"punct","subj":"T26997","obj":"T26989"},{"id":"R7844","pred":"advmod","subj":"T26998","obj":"T26989"},{"id":"R7845","pred":"punct","subj":"T26999","obj":"T26989"},{"id":"R7846","pred":"compound","subj":"T27000","obj":"T27001"},{"id":"R7847","pred":"nsubjpass","subj":"T27001","obj":"T26989"},{"id":"R7848","pred":"auxpass","subj":"T27002","obj":"T26989"},{"id":"R7849","pred":"prep","subj":"T27003","obj":"T26989"},{"id":"R7850","pred":"quantmod","subj":"T27004","obj":"T27005"},{"id":"R7851","pred":"nummod","subj":"T27005","obj":"T27006"},{"id":"R7852","pred":"pobj","subj":"T27006","obj":"T27003"},{"id":"R7853","pred":"punct","subj":"T27007","obj":"T26989"},{"id":"R7854","pred":"prep","subj":"T27009","obj":"T27010"},{"id":"R7855","pred":"npadvmod","subj":"T27011","obj":"T27012"},{"id":"R7856","pred":"amod","subj":"T27012","obj":"T27013"},{"id":"R7857","pred":"pobj","subj":"T27013","obj":"T27009"},{"id":"R7858","pred":"compound","subj":"T27014","obj":"T27015"},{"id":"R7859","pred":"compound","subj":"T27015","obj":"T27017"},{"id":"R7860","pred":"punct","subj":"T27016","obj":"T27015"},{"id":"R7861","pred":"nsubj","subj":"T27017","obj":"T27010"},{"id":"R7862","pred":"advmod","subj":"T27018","obj":"T27019"},{"id":"R7863","pred":"acomp","subj":"T27019","obj":"T27010"},{"id":"R7864","pred":"punct","subj":"T27020","obj":"T27010"},{"id":"R7865","pred":"prep","subj":"T27021","obj":"T27010"},{"id":"R7866","pred":"cc","subj":"T27022","obj":"T27021"},{"id":"R7867","pred":"conj","subj":"T27023","obj":"T27021"},{"id":"R7868","pred":"amod","subj":"T27024","obj":"T27025"},{"id":"R7869","pred":"compound","subj":"T27025","obj":"T27027"},{"id":"R7870","pred":"punct","subj":"T27026","obj":"T27025"},{"id":"R7871","pred":"pobj","subj":"T27027","obj":"T27023"},{"id":"R7872","pred":"compound","subj":"T27028","obj":"T27027"},{"id":"R7873","pred":"punct","subj":"T27029","obj":"T27010"},{"id":"R7874","pred":"det","subj":"T27031","obj":"T27032"},{"id":"R7875","pred":"nsubj","subj":"T27032","obj":"T27034"},{"id":"R7876","pred":"amod","subj":"T27033","obj":"T27032"},{"id":"R7877","pred":"det","subj":"T27035","obj":"T27036"},{"id":"R7878","pred":"dobj","subj":"T27036","obj":"T27034"},{"id":"R7879","pred":"compound","subj":"T27037","obj":"T27036"},{"id":"R7880","pred":"prep","subj":"T27038","obj":"T27036"},{"id":"R7881","pred":"advmod","subj":"T27039","obj":"T27040"},{"id":"R7882","pred":"pobj","subj":"T27040","obj":"T27038"},{"id":"R7883","pred":"advmod","subj":"T27041","obj":"T27040"},{"id":"R7884","pred":"punct","subj":"T27042","obj":"T27034"}],"text":"Quantitative analysis of BAT-gal reporter expression in Pygo1/Pygo2 E18.5 kidney extracts. Transgene-specific beta-galactosidase was quantified by ELISA analysis. Pygo1+/-/Pygo2+/+, Pygo1+/-/Pygo2+/- and Pygo1+/-/Pygo2+/+ kidneys all showed similar levels of BAT-gal expression. In the Pygo2 hetereozygote, Pygo1 homozygous mutants, however, reporter expression was reduced by about 50%. In Pygo2 homozygous mutants BAT-gal expression was uniformly low, with or without wild-type Pygo1 alleles. Each experimental group included a sample size of at least four."}
craft-ca-core-ex-dev
{"project":"craft-ca-core-ex-dev","denotations":[{"id":"T26916","span":{"begin":486,"end":493},"obj":"SO_EXT:0001023"},{"id":"T26915","span":{"begin":480,"end":485},"obj":"PR_EXT:000013523"},{"id":"T26914","span":{"begin":470,"end":479},"obj":"SO_EXT:wild_type_entity_or_quality"},{"id":"T26913","span":{"begin":424,"end":434},"obj":"GO:0010467"},{"id":"T26912","span":{"begin":420,"end":423},"obj":"GO_EXT:0015925"},{"id":"T26911","span":{"begin":408,"end":415},"obj":"SO_EXT:sequence_altered_entity"},{"id":"T26910","span":{"begin":391,"end":396},"obj":"PR_EXT:000013524"},{"id":"T26909","span":{"begin":351,"end":361},"obj":"GO:0010467"},{"id":"T26908","span":{"begin":324,"end":331},"obj":"SO_EXT:sequence_altered_entity"},{"id":"T26907","span":{"begin":307,"end":312},"obj":"PR_EXT:000013523"},{"id":"T26906","span":{"begin":286,"end":291},"obj":"PR_EXT:000013524"},{"id":"T26905","span":{"begin":267,"end":277},"obj":"GO:0010467"},{"id":"T26904","span":{"begin":263,"end":266},"obj":"GO_EXT:0015925"},{"id":"T26903","span":{"begin":222,"end":229},"obj":"UBERON:0002113"},{"id":"T26902","span":{"begin":220,"end":221},"obj":"SO_EXT:normal_or_wild_type_or_present"},{"id":"T26901","span":{"begin":218,"end":219},"obj":"SO_EXT:normal_or_wild_type_or_present"},{"id":"T26900","span":{"begin":213,"end":218},"obj":"PR_EXT:000013524"},{"id":"T26899","span":{"begin":211,"end":212},"obj":"SO_EXT:sequence_nullness_or_absence"},{"id":"T26898","span":{"begin":209,"end":210},"obj":"SO_EXT:normal_or_wild_type_or_present"},{"id":"T26897","span":{"begin":204,"end":209},"obj":"PR_EXT:000013523"},{"id":"T26896","span":{"begin":198,"end":199},"obj":"SO_EXT:sequence_nullness_or_absence"},{"id":"T26895","span":{"begin":196,"end":197},"obj":"SO_EXT:normal_or_wild_type_or_present"},{"id":"T26894","span":{"begin":191,"end":196},"obj":"PR_EXT:000013524"},{"id":"T26893","span":{"begin":189,"end":190},"obj":"SO_EXT:sequence_nullness_or_absence"},{"id":"T26892","span":{"begin":187,"end":188},"obj":"SO_EXT:normal_or_wild_type_or_present"},{"id":"T26891","span":{"begin":182,"end":187},"obj":"PR_EXT:000013523"},{"id":"T26890","span":{"begin":179,"end":180},"obj":"SO_EXT:normal_or_wild_type_or_present"},{"id":"T26889","span":{"begin":177,"end":178},"obj":"SO_EXT:normal_or_wild_type_or_present"},{"id":"T26888","span":{"begin":172,"end":177},"obj":"PR_EXT:000013524"},{"id":"T26887","span":{"begin":170,"end":171},"obj":"SO_EXT:sequence_nullness_or_absence"},{"id":"T26886","span":{"begin":168,"end":169},"obj":"SO_EXT:normal_or_wild_type_or_present"},{"id":"T26885","span":{"begin":163,"end":168},"obj":"PR_EXT:000013523"},{"id":"T26884","span":{"begin":110,"end":128},"obj":"GO_EXT:0004565"},{"id":"T26883","span":{"begin":91,"end":100},"obj":"SO_EXT:0000902"},{"id":"T26882","span":{"begin":74,"end":80},"obj":"UBERON:0002113"},{"id":"T26881","span":{"begin":62,"end":67},"obj":"PR_EXT:000013524"},{"id":"T26880","span":{"begin":56,"end":61},"obj":"PR_EXT:000013523"},{"id":"T26879","span":{"begin":42,"end":52},"obj":"GO:0010467"},{"id":"T26878","span":{"begin":29,"end":32},"obj":"GO_EXT:0015925"}],"text":"Quantitative analysis of BAT-gal reporter expression in Pygo1/Pygo2 E18.5 kidney extracts. Transgene-specific beta-galactosidase was quantified by ELISA analysis. Pygo1+/-/Pygo2+/+, Pygo1+/-/Pygo2+/- and Pygo1+/-/Pygo2+/+ kidneys all showed similar levels of BAT-gal expression. In the Pygo2 hetereozygote, Pygo1 homozygous mutants, however, reporter expression was reduced by about 50%. In Pygo2 homozygous mutants BAT-gal expression was uniformly low, with or without wild-type Pygo1 alleles. Each experimental group included a sample size of at least four."}
craft-ca-core-dev
{"project":"craft-ca-core-dev","denotations":[{"id":"T26870","span":{"begin":267,"end":277},"obj":"GO:0010467"},{"id":"T26869","span":{"begin":222,"end":229},"obj":"UBERON:0002113"},{"id":"T26868","span":{"begin":213,"end":218},"obj":"PR:000013524"},{"id":"T26867","span":{"begin":204,"end":209},"obj":"PR:000013523"},{"id":"T26866","span":{"begin":191,"end":196},"obj":"PR:000013524"},{"id":"T26865","span":{"begin":182,"end":187},"obj":"PR:000013523"},{"id":"T26864","span":{"begin":172,"end":177},"obj":"PR:000013524"},{"id":"T26863","span":{"begin":163,"end":168},"obj":"PR:000013523"},{"id":"T26862","span":{"begin":91,"end":100},"obj":"SO:0000902"},{"id":"T26861","span":{"begin":74,"end":80},"obj":"UBERON:0002113"},{"id":"T26860","span":{"begin":62,"end":67},"obj":"PR:000013524"},{"id":"T26859","span":{"begin":56,"end":61},"obj":"PR:000013523"},{"id":"T26858","span":{"begin":42,"end":52},"obj":"GO:0010467"},{"id":"T26877","span":{"begin":486,"end":493},"obj":"SO:0001023"},{"id":"T26876","span":{"begin":480,"end":485},"obj":"PR:000013523"},{"id":"T26875","span":{"begin":424,"end":434},"obj":"GO:0010467"},{"id":"T26874","span":{"begin":391,"end":396},"obj":"PR:000013524"},{"id":"T26873","span":{"begin":351,"end":361},"obj":"GO:0010467"},{"id":"T26872","span":{"begin":307,"end":312},"obj":"PR:000013523"},{"id":"T26871","span":{"begin":286,"end":291},"obj":"PR:000013524"}],"text":"Quantitative analysis of BAT-gal reporter expression in Pygo1/Pygo2 E18.5 kidney extracts. Transgene-specific beta-galactosidase was quantified by ELISA analysis. Pygo1+/-/Pygo2+/+, Pygo1+/-/Pygo2+/- and Pygo1+/-/Pygo2+/+ kidneys all showed similar levels of BAT-gal expression. In the Pygo2 hetereozygote, Pygo1 homozygous mutants, however, reporter expression was reduced by about 50%. In Pygo2 homozygous mutants BAT-gal expression was uniformly low, with or without wild-type Pygo1 alleles. Each experimental group included a sample size of at least four."}