Table 2 Out of 206 gene/TF interactions found in the RegulonDB (Salgado et al. 2004) and EcoCyc databases, 44 scored higher than the imposed threshold. TF Gene Final Score predicted sign actual sign 1 ArcA-Phosphorylated yfiD 1.670768591 up up 2 ArgR-L-arginine argG 2.624262246 down down 3 CRP-cAMP ugpQ 2.085693237 up up 4 CRP-cAMP ugpC 1.438960585 up up 5 CRP-cAMP ugpB 1.527292985 up up 6 CRP-cAMP rhaB 2.909109432 up up 7 CRP-cAMP cytR 2.119101207 up up 8 CRP-cAMP fis 1.887330412 up/down up/down 9 FhlA-Formate hyfA 2.509458668 up up 10 Fis relA 1.595459732 up up 11 GntR idnK 1.591861262 down down 12 Lrp livJ 1.380451883 down down 13 MalT-Maltotriose-ATP malZ 1.44543234 up up 14 NarL-Phosphorylated fdhF 2.059069186 up up 15 NtrC-Phosphorylated astC 2.256990592 up up 16 ArgR-L-arginine astC 1.505034 down up 17 CRP-cAMP nagE 1.501987378 up/down up 18 CRP-cAMP rpoS 2.319114034 up/down down 19 OmpR-Phosphorylated nmpC 1.572242248 up down 20 ArcA-Phosphorylated aceE 1.998375233 down down 21 ArcA-Phosphorylated appC 2.066734924 up up 22 CRP-cAMP entC 2.93996178 up up 23 CRP-cAMP fepA 3.821927836 up up 24 CRP-cAMP fumA 3.496049117 up up 25 CRP-cAMP gadB 2.197333426 down down 26 CRP-cAMP galP 2.37334941 up up 27 CRP-cAMP gapA 1.811986697 up up 28 CRP-cAMP ompF 1.315706561 up up 29 FruR acnA 1.633652931 up up 30 FruR glk 2.112996214 down down 31 GadE gadB 1.973757857 up up 32 Hns gadB 1.78680429 down down 33 LexA uvrC 1.590391147 down down 34 MetJ-S-adenosylmethionine metE 3.264214502 down down 35 MetJ-S-adenosylmethionine metR 1.611151228 down down 36 MetR-Homocysteine metE 3.45265202 up up 37 PhoP-Phosphorylated rstA 1.6475787 up up 38 CRP-cAMP prpR 3.147648347 up/down up 39 Fnr fdhF 1.985483486 up/down up 40 CRP-cAMP nagE 1.501987378 up/down up 41 NarP-Phosphorylated fdhFp 1.645007039 up/down up 42 NarP-Phosphorylated fdnG 2.778075473 up/down down 43 Fnr dcuS 1.359810967 down up 44 Fnr purM 1.427076396 up down The p-value (using binary distribution) is found to be less than 1.0e-50.