After we performed the calculations we found 206 more gene/TF interactions in the RegulonDB and EcoCyc databases that were not included in the training set. 44 out of 206 regulatory interactions were predicted by our methodology. Out of 44 interactions, the nature of regulation was correctly predicted for 33 of them. Regulation type couldn't be obtained for 7 interactions. Regulation nature was incorrectly predicted for the remaining 4 interactions (Table 2). We obtained the p-value for predicting at least 44 out of 206 gene/TF interactions to be less than 1.0e-50 (expected proportion = 3.5e-04, number observed = 44, sample size = 3694).