The suggested TRN includes 3694 new gene/TF interactions. If the training TRN is a random sampling of the actual TRN, then, for a sufficiently large training TRN, it is expected to exhibit the basic functional properties of the actual TRN. The suggested TRN is denser than the training TRN. However, as illustrated in Fig. 10, probability distributions for the number of gene/TF interactions per gene for both the training and suggested TRNs show a high degree of similarity. Clearly, our training set is vastly incomplete. Not only we don't have any regulatory information for over 3,000 genes, but we likely know only a fraction of the number of TFs regulating those 984 genes for which at least one regulating TF is known. Therefore, the true E. coli TRN is likely to be denser, as predicted here.