Id |
Subject |
Object |
Predicate |
Lexical cue |
T7971 |
8-10 |
PRP |
denotes |
We |
T7972 |
11-15 |
VBD |
denotes |
used |
T7973 |
16-20 |
NN |
denotes |
gene |
T7974 |
21-30 |
NN |
denotes |
targeting |
T7975 |
31-33 |
IN |
denotes |
in |
T7976 |
34-36 |
NN |
denotes |
ES |
T7977 |
37-42 |
NNS |
denotes |
cells |
T7978 |
43-45 |
TO |
denotes |
to |
T7979 |
46-52 |
VB |
denotes |
delete |
T7980 |
53-57 |
NN |
denotes |
exon |
T7981 |
58-59 |
CD |
denotes |
3 |
T7982 |
60-62 |
IN |
denotes |
in |
T7983 |
63-66 |
DT |
denotes |
the |
T7984 |
74-78 |
NN |
denotes |
gene |
T7985 |
67-73 |
NN |
denotes |
Chaf1a |
T7986 |
78-80 |
, |
denotes |
, |
T7987 |
80-85 |
WDT |
denotes |
which |
T7988 |
86-93 |
VBZ |
denotes |
encodes |
T7989 |
94-101 |
NN |
denotes |
p150CAF |
T7990 |
101-102 |
HYPH |
denotes |
- |
T7991 |
102-103 |
CD |
denotes |
1 |
T7992 |
104-105 |
-LRB- |
denotes |
( |
T7993 |
112-114 |
NN |
denotes |
1A |
T7994 |
105-111 |
NN |
denotes |
Figure |
T7995 |
114-115 |
-RRB- |
denotes |
) |
T7996 |
115-116 |
. |
denotes |
. |
T7997 |
116-254 |
sentence |
denotes |
Chaf1a +/− mice were born at a Mendelian frequency, were of normal size and weight, displayed no obvious abnormalities, and were fertile. |
T7998 |
117-123 |
NN |
denotes |
Chaf1a |
T7999 |
128-132 |
NNS |
denotes |
mice |
T8000 |
124-125 |
SYM |
denotes |
+ |
T8001 |
125-126 |
HYPH |
denotes |
/ |
T8002 |
126-127 |
SYM |
denotes |
− |
T8003 |
138-142 |
VBN |
denotes |
born |
T8004 |
133-137 |
VBD |
denotes |
were |
T8005 |
143-145 |
IN |
denotes |
at |
T8006 |
146-147 |
DT |
denotes |
a |
T8007 |
158-167 |
NN |
denotes |
frequency |
T8008 |
148-157 |
JJ |
denotes |
Mendelian |
T8009 |
167-169 |
, |
denotes |
, |
T8010 |
169-173 |
VBD |
denotes |
were |
T8011 |
174-176 |
IN |
denotes |
of |
T8012 |
177-183 |
JJ |
denotes |
normal |
T8013 |
184-188 |
NN |
denotes |
size |
T8014 |
189-192 |
CC |
denotes |
and |
T8015 |
193-199 |
NN |
denotes |
weight |
T8016 |
199-201 |
, |
denotes |
, |
T8017 |
201-210 |
VBD |
denotes |
displayed |
T8018 |
211-213 |
DT |
denotes |
no |
T8019 |
222-235 |
NNS |
denotes |
abnormalities |
T8020 |
214-221 |
JJ |
denotes |
obvious |
T8021 |
235-237 |
, |
denotes |
, |
T8022 |
237-240 |
CC |
denotes |
and |
T8023 |
241-245 |
VBD |
denotes |
were |
T8024 |
246-253 |
JJ |
denotes |
fertile |
T8025 |
253-254 |
. |
denotes |
. |
T8026 |
254-332 |
sentence |
denotes |
Crossing heterozygous mice failed to generate viable newborn Chaf1a −/− mice. |
T8027 |
255-263 |
VBG |
denotes |
Crossing |
T8028 |
282-288 |
VBD |
denotes |
failed |
T8029 |
264-276 |
JJ |
denotes |
heterozygous |
T8030 |
277-281 |
NNS |
denotes |
mice |
T8031 |
289-291 |
TO |
denotes |
to |
T8032 |
292-300 |
VB |
denotes |
generate |
T8033 |
301-307 |
JJ |
denotes |
viable |
T8034 |
327-331 |
NNS |
denotes |
mice |
T8035 |
308-315 |
JJ |
denotes |
newborn |
T8036 |
316-322 |
NN |
denotes |
Chaf1a |
T8037 |
323-324 |
SYM |
denotes |
− |
T8038 |
324-325 |
HYPH |
denotes |
/ |
T8039 |
325-326 |
SYM |
denotes |
− |
T8040 |
331-332 |
. |
denotes |
. |
T8041 |
332-432 |
sentence |
denotes |
Furthermore, no homozygous Chaf1a −/− embryos were detected at any of the post-implantation stages. |
T8042 |
333-344 |
RB |
denotes |
Furthermore |
T8043 |
384-392 |
VBN |
denotes |
detected |
T8044 |
344-346 |
, |
denotes |
, |
T8045 |
346-348 |
DT |
denotes |
no |
T8046 |
371-378 |
NNS |
denotes |
embryos |
T8047 |
349-359 |
JJ |
denotes |
homozygous |
T8048 |
360-366 |
NN |
denotes |
Chaf1a |
T8049 |
367-368 |
SYM |
denotes |
− |
T8050 |
368-369 |
HYPH |
denotes |
/ |
T8051 |
369-370 |
SYM |
denotes |
− |
T8052 |
379-383 |
VBD |
denotes |
were |
T8053 |
393-395 |
IN |
denotes |
at |
T8054 |
396-399 |
DT |
denotes |
any |
T8055 |
400-402 |
IN |
denotes |
of |
T8056 |
403-406 |
DT |
denotes |
the |
T8057 |
425-431 |
NNS |
denotes |
stages |
T8058 |
407-424 |
JJ |
denotes |
post-implantation |
T8059 |
431-432 |
. |
denotes |
. |
T8060 |
432-534 |
sentence |
denotes |
However, we could detect Chaf1a −/− embryos at embryonic day 4 (E4) using a PCR strategy (Figure 1B). |
T8061 |
433-440 |
RB |
denotes |
However |
T8062 |
451-457 |
VB |
denotes |
detect |
T8063 |
440-442 |
, |
denotes |
, |
T8064 |
442-444 |
PRP |
denotes |
we |
T8065 |
445-450 |
MD |
denotes |
could |
T8066 |
458-464 |
NN |
denotes |
Chaf1a |
T8067 |
469-476 |
NNS |
denotes |
embryos |
T8068 |
465-466 |
SYM |
denotes |
− |
T8069 |
466-467 |
HYPH |
denotes |
/ |
T8070 |
467-468 |
SYM |
denotes |
− |
T8071 |
477-479 |
IN |
denotes |
at |
T8072 |
480-489 |
JJ |
denotes |
embryonic |
T8073 |
490-493 |
NN |
denotes |
day |
T8074 |
494-495 |
CD |
denotes |
4 |
T8075 |
496-497 |
-LRB- |
denotes |
( |
T8076 |
497-499 |
NN |
denotes |
E4 |
T8077 |
499-500 |
-RRB- |
denotes |
) |
T8078 |
501-506 |
VBG |
denotes |
using |
T8079 |
507-508 |
DT |
denotes |
a |
T8080 |
513-521 |
NN |
denotes |
strategy |
T8081 |
509-512 |
NN |
denotes |
PCR |
T8082 |
522-523 |
-LRB- |
denotes |
( |
T8083 |
530-532 |
NN |
denotes |
1B |
T8084 |
523-529 |
NN |
denotes |
Figure |
T8085 |
532-533 |
-RRB- |
denotes |
) |
T8086 |
533-534 |
. |
denotes |
. |
T8087 |
534-715 |
sentence |
denotes |
They represent only 10% of E4 embryos obtained from Chaf1a +/− mice intercrosses, suggesting that more than half of the homozygous mutant embryos had degenerated before this stage. |
T8088 |
535-539 |
PRP |
denotes |
They |
T8089 |
540-549 |
VBP |
denotes |
represent |
T8090 |
550-554 |
RB |
denotes |
only |
T8091 |
555-557 |
CD |
denotes |
10 |
T8092 |
557-558 |
NN |
denotes |
% |
T8093 |
559-561 |
IN |
denotes |
of |
T8094 |
562-564 |
NN |
denotes |
E4 |
T8095 |
565-572 |
NNS |
denotes |
embryos |
T8096 |
573-581 |
VBN |
denotes |
obtained |
T8097 |
582-586 |
IN |
denotes |
from |
T8098 |
587-593 |
NN |
denotes |
Chaf1a |
T8099 |
603-615 |
NNS |
denotes |
intercrosses |
T8100 |
594-595 |
SYM |
denotes |
+ |
T8101 |
595-596 |
HYPH |
denotes |
/ |
T8102 |
596-597 |
SYM |
denotes |
− |
T8103 |
598-602 |
NNS |
denotes |
mice |
T8104 |
615-617 |
, |
denotes |
, |
T8105 |
617-627 |
VBG |
denotes |
suggesting |
T8106 |
628-632 |
IN |
denotes |
that |
T8107 |
685-696 |
VBN |
denotes |
degenerated |
T8108 |
633-637 |
JJR |
denotes |
more |
T8109 |
643-647 |
NN |
denotes |
half |
T8110 |
638-642 |
IN |
denotes |
than |
T8111 |
648-650 |
IN |
denotes |
of |
T8112 |
651-654 |
DT |
denotes |
the |
T8113 |
673-680 |
NNS |
denotes |
embryos |
T8114 |
655-665 |
JJ |
denotes |
homozygous |
T8115 |
666-672 |
NN |
denotes |
mutant |
T8116 |
681-684 |
VBD |
denotes |
had |
T8117 |
697-703 |
IN |
denotes |
before |
T8118 |
704-708 |
DT |
denotes |
this |
T8119 |
709-714 |
NN |
denotes |
stage |
T8120 |
714-715 |
. |
denotes |
. |
T8121 |
715-881 |
sentence |
denotes |
Moreover, mutant embryos contained only eight to 16 cells at the E4 stage, instead of 32 cells observed for wild-type or heterozygous blastocysts (Figure 1C and 1D). |
T8122 |
716-724 |
RB |
denotes |
Moreover |
T8123 |
741-750 |
VBD |
denotes |
contained |
T8124 |
724-726 |
, |
denotes |
, |
T8125 |
726-732 |
NN |
denotes |
mutant |
T8126 |
733-740 |
NNS |
denotes |
embryos |
T8127 |
751-755 |
RB |
denotes |
only |
T8128 |
765-767 |
CD |
denotes |
16 |
T8129 |
756-761 |
CD |
denotes |
eight |
T8130 |
762-764 |
IN |
denotes |
to |
T8131 |
768-773 |
NNS |
denotes |
cells |
T8132 |
774-776 |
IN |
denotes |
at |
T8133 |
777-780 |
DT |
denotes |
the |
T8134 |
784-789 |
NN |
denotes |
stage |
T8135 |
781-783 |
NN |
denotes |
E4 |
T8136 |
789-791 |
, |
denotes |
, |
T8137 |
791-798 |
RB |
denotes |
instead |
T8138 |
799-801 |
IN |
denotes |
of |
T8139 |
802-804 |
CD |
denotes |
32 |
T8140 |
805-810 |
NNS |
denotes |
cells |
T8141 |
811-819 |
VBN |
denotes |
observed |
T8142 |
820-823 |
IN |
denotes |
for |
T8143 |
824-828 |
JJ |
denotes |
wild |
T8144 |
829-833 |
NN |
denotes |
type |
T8145 |
828-829 |
HYPH |
denotes |
- |
T8146 |
850-861 |
NNS |
denotes |
blastocysts |
T8147 |
834-836 |
CC |
denotes |
or |
T8148 |
837-849 |
JJ |
denotes |
heterozygous |
T8149 |
862-863 |
-LRB- |
denotes |
( |
T8150 |
870-872 |
NN |
denotes |
1C |
T8151 |
863-869 |
NN |
denotes |
Figure |
T8152 |
873-876 |
CC |
denotes |
and |
T8153 |
877-879 |
NN |
denotes |
1D |
T8154 |
879-880 |
-RRB- |
denotes |
) |
T8155 |
880-881 |
. |
denotes |
. |
T8156 |
881-1012 |
sentence |
denotes |
Further analysis by light microscopy using immunofluorescence (IF) allowed us to compare wild-type and p150CAF-1-depleted embryos. |
T8157 |
882-889 |
JJ |
denotes |
Further |
T8158 |
890-898 |
NN |
denotes |
analysis |
T8159 |
949-956 |
VBD |
denotes |
allowed |
T8160 |
899-901 |
IN |
denotes |
by |
T8161 |
902-907 |
NN |
denotes |
light |
T8162 |
908-918 |
NN |
denotes |
microscopy |
T8163 |
919-924 |
VBG |
denotes |
using |
T8164 |
925-943 |
NN |
denotes |
immunofluorescence |
T8165 |
944-945 |
-LRB- |
denotes |
( |
T8166 |
945-947 |
NN |
denotes |
IF |
T8167 |
947-948 |
-RRB- |
denotes |
) |
T8168 |
957-959 |
PRP |
denotes |
us |
T8169 |
963-970 |
VB |
denotes |
compare |
T8170 |
960-962 |
TO |
denotes |
to |
T8171 |
971-975 |
JJ |
denotes |
wild |
T8172 |
976-980 |
NN |
denotes |
type |
T8173 |
975-976 |
HYPH |
denotes |
- |
T8174 |
1004-1011 |
NNS |
denotes |
embryos |
T8175 |
981-984 |
CC |
denotes |
and |
T8176 |
985-992 |
NN |
denotes |
p150CAF |
T8177 |
995-1003 |
VBN |
denotes |
depleted |
T8178 |
992-993 |
HYPH |
denotes |
- |
T8179 |
993-994 |
CD |
denotes |
1 |
T8180 |
994-995 |
HYPH |
denotes |
- |
T8181 |
1011-1012 |
. |
denotes |
. |
T8182 |
1012-1209 |
sentence |
denotes |
A loss of p150CAF-1 staining could not be detected before the eight-cell stage (unpublished data) suggesting that maternally contributed protein is present in the embryo up to the four-cell stage. |
T8183 |
1013-1014 |
DT |
denotes |
A |
T8184 |
1015-1019 |
NN |
denotes |
loss |
T8185 |
1055-1063 |
VBN |
denotes |
detected |
T8186 |
1020-1022 |
IN |
denotes |
of |
T8187 |
1023-1030 |
NN |
denotes |
p150CAF |
T8188 |
1033-1041 |
NN |
denotes |
staining |
T8189 |
1030-1031 |
HYPH |
denotes |
- |
T8190 |
1031-1032 |
CD |
denotes |
1 |
T8191 |
1042-1047 |
MD |
denotes |
could |
T8192 |
1048-1051 |
RB |
denotes |
not |
T8193 |
1052-1054 |
VB |
denotes |
be |
T8194 |
1064-1070 |
IN |
denotes |
before |
T8195 |
1071-1074 |
DT |
denotes |
the |
T8196 |
1086-1091 |
NN |
denotes |
stage |
T8197 |
1075-1080 |
CD |
denotes |
eight |
T8198 |
1081-1085 |
NN |
denotes |
cell |
T8199 |
1080-1081 |
HYPH |
denotes |
- |
T8200 |
1092-1093 |
-LRB- |
denotes |
( |
T8201 |
1105-1109 |
NNS |
denotes |
data |
T8202 |
1093-1104 |
JJ |
denotes |
unpublished |
T8203 |
1109-1110 |
-RRB- |
denotes |
) |
T8204 |
1111-1121 |
VBG |
denotes |
suggesting |
T8205 |
1122-1126 |
IN |
denotes |
that |
T8206 |
1158-1160 |
VBZ |
denotes |
is |
T8207 |
1127-1137 |
RB |
denotes |
maternally |
T8208 |
1138-1149 |
VBN |
denotes |
contributed |
T8209 |
1150-1157 |
NN |
denotes |
protein |
T8210 |
1161-1168 |
JJ |
denotes |
present |
T8211 |
1169-1171 |
IN |
denotes |
in |
T8212 |
1172-1175 |
DT |
denotes |
the |
T8213 |
1176-1182 |
NN |
denotes |
embryo |
T8214 |
1183-1185 |
IN |
denotes |
up |
T8215 |
1186-1188 |
IN |
denotes |
to |
T8216 |
1189-1192 |
DT |
denotes |
the |
T8217 |
1203-1208 |
NN |
denotes |
stage |
T8218 |
1193-1197 |
CD |
denotes |
four |
T8219 |
1198-1202 |
NN |
denotes |
cell |
T8220 |
1197-1198 |
HYPH |
denotes |
- |
T8221 |
1208-1209 |
. |
denotes |
. |
T8222 |
1209-1341 |
sentence |
denotes |
Thus, depletion of p150CAF-1 protein from this stage allows a maximum of two additional cell divisions before developmental arrest. |
T8223 |
1210-1214 |
RB |
denotes |
Thus |
T8224 |
1263-1269 |
VBZ |
denotes |
allows |
T8225 |
1214-1216 |
, |
denotes |
, |
T8226 |
1216-1225 |
NN |
denotes |
depletion |
T8227 |
1226-1228 |
IN |
denotes |
of |
T8228 |
1229-1236 |
NN |
denotes |
p150CAF |
T8229 |
1239-1246 |
NN |
denotes |
protein |
T8230 |
1236-1237 |
HYPH |
denotes |
- |
T8231 |
1237-1238 |
CD |
denotes |
1 |
T8232 |
1247-1251 |
IN |
denotes |
from |
T8233 |
1252-1256 |
DT |
denotes |
this |
T8234 |
1257-1262 |
NN |
denotes |
stage |
T8235 |
1270-1271 |
DT |
denotes |
a |
T8236 |
1272-1279 |
NN |
denotes |
maximum |
T8237 |
1280-1282 |
IN |
denotes |
of |
T8238 |
1283-1286 |
CD |
denotes |
two |
T8239 |
1303-1312 |
NNS |
denotes |
divisions |
T8240 |
1287-1297 |
JJ |
denotes |
additional |
T8241 |
1298-1302 |
NN |
denotes |
cell |
T8242 |
1313-1319 |
IN |
denotes |
before |
T8243 |
1320-1333 |
JJ |
denotes |
developmental |
T8244 |
1334-1340 |
NN |
denotes |
arrest |
T8245 |
1340-1341 |
. |
denotes |
. |
T8246 |
1341-4028 |
sentence |
denotes |
Figure 1 Loss of p150CAF-1 Function Leads to Early Developmental Arrest and Alteration of Heterochromatin Organization
(A) Generation of Chaf1a +/− mice by homologous recombination in ES cells. (Top) Scheme of the p150CAF1 protein indicating the interacting domains (ID) for HP1 and p60CAF-1, and the acidic domain (AD). (Middle) Structure of the murine Chaf1a gene. Blocks and lines represent exons and introns, respectively. A star indicates the translation initiation site. Below is shown the Chaf1a targeting vector, which includes genomic DNA homology regions (5 HR and 3 HR), the diphtheria toxin (DT), and neomycin selection genes. Recombined (Rec) ES cells and mice were identified by Southern blot using ScaI (S) and the indicated probe.
(B) Breeding strategy used for the production of E4 Chaf1a −/− embryos. Genotyping was performed by PCR using the three oligonucleotide primers indicated in (A) (1, 2, and 3). An example of the result of a genotyping experiment is shown.
(C) Immunodetection of p150CAF-1 (green) and HP1α (red) in E4 embryos derived from Chaf1a +/− intercrosses. Because Chaf1a +/+ and Chaf1a +/− embryos both stain positively for the presence of p150CAF-1, they are both designated as wild-type. p150CAF-1 expression, which indicates ongoing S phase, can be detected in most cells within the wild-type blastocyst (upper panel). The lower panel shows a 12-cell embryo labeling negatively for p150CAF-1. Only nonspecific background labeling can be observed. In each panel, the right-hand image shows the merge between the HP1α fluorescence and DAPI-stained DNA in blue. Pink color indicates the association of HP1 with DAPI-dense material. The bottom of each panel shows the magnification of a nucleus selected from the above embryo (white square). The arrowhead indicates the typical heterochromatin foci revealed by DAPI and HP1α staining in wild-type embryos. These foci are not visible in Chaf1a −/− embryos. DAPI and HP1α staining are diffuse within the nucleus of p150CAF-1-depleted embryos, with enrichment at the nuclear periphery, revealing abnormal heterochromatin organization. Scale bars represent 10 μm.
(E) Wild-type embryos isolated at embryonic day 2 (E2, two cells), E2.5 (four cells), E3 (eight cells), E3.5 (16 cells), and E4 (32 cells) are shown. Heterochromatin was monitored by DAPI staining (upper panel) and HP1 immunolabeling (in red, lower panel).
(F) Magnification of a nucleus (DAPI-stained) representative of each stage. The right-hand panel shows the nucleus of an ES cell. Scale bar represents 10 μm. Strikingly, we found that the nuclear organization of heterochromatin appeared abnormal in the nuclei of Chaf1a −/− embryos. |
T8247 |
3904-3914 |
RB |
denotes |
Strikingly |
T8248 |
3919-3924 |
VBD |
denotes |
found |
T8249 |
3914-3916 |
, |
denotes |
, |
T8250 |
3916-3918 |
PRP |
denotes |
we |
T8251 |
3925-3929 |
IN |
denotes |
that |
T8252 |
3974-3982 |
VBD |
denotes |
appeared |
T8253 |
3930-3933 |
DT |
denotes |
the |
T8254 |
3942-3954 |
NN |
denotes |
organization |
T8255 |
3934-3941 |
JJ |
denotes |
nuclear |
T8256 |
3955-3957 |
IN |
denotes |
of |
T8257 |
3958-3973 |
NN |
denotes |
heterochromatin |
T8258 |
3983-3991 |
JJ |
denotes |
abnormal |
T8259 |
3992-3994 |
IN |
denotes |
in |
T8260 |
3995-3998 |
DT |
denotes |
the |
T8261 |
3999-4005 |
NNS |
denotes |
nuclei |
T8262 |
4006-4008 |
IN |
denotes |
of |
T8263 |
4009-4015 |
NN |
denotes |
Chaf1a |
T8264 |
4020-4027 |
NNS |
denotes |
embryos |
T8265 |
4016-4017 |
SYM |
denotes |
− |
T8266 |
4017-4018 |
HYPH |
denotes |
/ |
T8267 |
4018-4019 |
SYM |
denotes |
− |
T8268 |
4027-4028 |
. |
denotes |
. |
T8269 |
4028-4225 |
sentence |
denotes |
Pericentric heterochromatin, the major component of constitutive heterochromatin, can easily be visualized in interphase nuclei by the fluorochrome DAPI and by immunostaining with HP1α [16,17–19]. |
T8270 |
4029-4040 |
JJ |
denotes |
Pericentric |
T8271 |
4041-4056 |
NN |
denotes |
heterochromatin |
T8272 |
4125-4135 |
VBN |
denotes |
visualized |
T8273 |
4056-4058 |
, |
denotes |
, |
T8274 |
4058-4061 |
DT |
denotes |
the |
T8275 |
4068-4077 |
NN |
denotes |
component |
T8276 |
4062-4067 |
JJ |
denotes |
major |
T8277 |
4078-4080 |
IN |
denotes |
of |
T8278 |
4081-4093 |
JJ |
denotes |
constitutive |
T8279 |
4094-4109 |
NN |
denotes |
heterochromatin |
T8280 |
4109-4111 |
, |
denotes |
, |
T8281 |
4111-4114 |
MD |
denotes |
can |
T8282 |
4115-4121 |
RB |
denotes |
easily |
T8283 |
4122-4124 |
VB |
denotes |
be |
T8284 |
4136-4138 |
IN |
denotes |
in |
T8285 |
4139-4149 |
NN |
denotes |
interphase |
T8286 |
4150-4156 |
NNS |
denotes |
nuclei |
T8287 |
4157-4159 |
IN |
denotes |
by |
T8288 |
4160-4163 |
DT |
denotes |
the |
T8289 |
4177-4181 |
NN |
denotes |
DAPI |
T8290 |
4164-4176 |
NN |
denotes |
fluorochrome |
T8291 |
4182-4185 |
CC |
denotes |
and |
T8292 |
4186-4188 |
IN |
denotes |
by |
T8293 |
4189-4203 |
VBG |
denotes |
immunostaining |
T8294 |
4204-4208 |
IN |
denotes |
with |
T8295 |
4209-4213 |
NN |
denotes |
HP1α |
T8296 |
4214-4215 |
-LRB- |
denotes |
[ |
T8297 |
4215-4217 |
CD |
denotes |
16 |
T8298 |
4217-4218 |
, |
denotes |
, |
T8299 |
4218-4220 |
CD |
denotes |
17 |
T8300 |
4220-4221 |
SYM |
denotes |
– |
T8301 |
4221-4223 |
CD |
denotes |
19 |
T8302 |
4223-4224 |
-RRB- |
denotes |
] |
T8303 |
4224-4225 |
. |
denotes |
. |
T8304 |
4225-4454 |
sentence |
denotes |
In wild-type blastocysts and in mouse somatic cells, pericentric heterochromatin domains cluster together and form higher-order chromatin structures called chromocenters [20], which are visualized as DAPI-dense foci (Figure 1C). |
T8305 |
4226-4228 |
IN |
denotes |
In |
T8306 |
4315-4322 |
VBP |
denotes |
cluster |
T8307 |
4229-4233 |
JJ |
denotes |
wild |
T8308 |
4234-4238 |
NN |
denotes |
type |
T8309 |
4233-4234 |
HYPH |
denotes |
- |
T8310 |
4239-4250 |
NNS |
denotes |
blastocysts |
T8311 |
4251-4254 |
CC |
denotes |
and |
T8312 |
4255-4257 |
IN |
denotes |
in |
T8313 |
4258-4263 |
NN |
denotes |
mouse |
T8314 |
4272-4277 |
NNS |
denotes |
cells |
T8315 |
4264-4271 |
JJ |
denotes |
somatic |
T8316 |
4277-4279 |
, |
denotes |
, |
T8317 |
4279-4290 |
JJ |
denotes |
pericentric |
T8318 |
4307-4314 |
NNS |
denotes |
domains |
T8319 |
4291-4306 |
NN |
denotes |
heterochromatin |
T8320 |
4323-4331 |
RB |
denotes |
together |
T8321 |
4332-4335 |
CC |
denotes |
and |
T8322 |
4336-4340 |
VBP |
denotes |
form |
T8323 |
4341-4347 |
JJR |
denotes |
higher |
T8324 |
4348-4353 |
NN |
denotes |
order |
T8325 |
4347-4348 |
HYPH |
denotes |
- |
T8326 |
4364-4374 |
NNS |
denotes |
structures |
T8327 |
4354-4363 |
NN |
denotes |
chromatin |
T8328 |
4375-4381 |
VBN |
denotes |
called |
T8329 |
4382-4395 |
NNS |
denotes |
chromocenters |
T8330 |
4396-4397 |
-LRB- |
denotes |
[ |
T8331 |
4397-4399 |
CD |
denotes |
20 |
T8332 |
4399-4400 |
-RRB- |
denotes |
] |
T8333 |
4400-4402 |
, |
denotes |
, |
T8334 |
4402-4407 |
WDT |
denotes |
which |
T8335 |
4412-4422 |
VBN |
denotes |
visualized |
T8336 |
4408-4411 |
VBP |
denotes |
are |
T8337 |
4423-4425 |
IN |
denotes |
as |
T8338 |
4426-4430 |
NN |
denotes |
DAPI |
T8339 |
4431-4436 |
JJ |
denotes |
dense |
T8340 |
4430-4431 |
HYPH |
denotes |
- |
T8341 |
4437-4441 |
NNS |
denotes |
foci |
T8342 |
4442-4443 |
-LRB- |
denotes |
( |
T8343 |
4450-4452 |
NN |
denotes |
1C |
T8344 |
4443-4449 |
NN |
denotes |
Figure |
T8345 |
4452-4453 |
-RRB- |
denotes |
) |
T8346 |
4453-4454 |
. |
denotes |
. |
T8347 |
4454-4524 |
sentence |
denotes |
These structures were not detected in Chaf1a −/− embryos (Figure 1D). |
T8348 |
4455-4460 |
DT |
denotes |
These |
T8349 |
4461-4471 |
NNS |
denotes |
structures |
T8350 |
4481-4489 |
VBN |
denotes |
detected |
T8351 |
4472-4476 |
VBD |
denotes |
were |
T8352 |
4477-4480 |
RB |
denotes |
not |
T8353 |
4490-4492 |
IN |
denotes |
in |
T8354 |
4493-4499 |
NN |
denotes |
Chaf1a |
T8355 |
4504-4511 |
NNS |
denotes |
embryos |
T8356 |
4500-4501 |
SYM |
denotes |
− |
T8357 |
4501-4502 |
HYPH |
denotes |
/ |
T8358 |
4502-4503 |
SYM |
denotes |
− |
T8359 |
4512-4513 |
-LRB- |
denotes |
( |
T8360 |
4520-4522 |
NN |
denotes |
1D |
T8361 |
4513-4519 |
NN |
denotes |
Figure |
T8362 |
4522-4523 |
-RRB- |
denotes |
) |
T8363 |
4523-4524 |
. |
denotes |
. |
T8364 |
4524-4679 |
sentence |
denotes |
Since chromatin architecture and higher-order structures are important for genome function [21], we decided to characterize this phenotype in more detail. |
T8365 |
4525-4530 |
IN |
denotes |
Since |
T8366 |
4582-4585 |
VBP |
denotes |
are |
T8367 |
4531-4540 |
NN |
denotes |
chromatin |
T8368 |
4541-4553 |
NN |
denotes |
architecture |
T8369 |
4554-4557 |
CC |
denotes |
and |
T8370 |
4558-4564 |
JJR |
denotes |
higher |
T8371 |
4565-4570 |
NN |
denotes |
order |
T8372 |
4564-4565 |
HYPH |
denotes |
- |
T8373 |
4571-4581 |
NNS |
denotes |
structures |
T8374 |
4625-4632 |
VBD |
denotes |
decided |
T8375 |
4586-4595 |
JJ |
denotes |
important |
T8376 |
4596-4599 |
IN |
denotes |
for |
T8377 |
4600-4606 |
NN |
denotes |
genome |
T8378 |
4607-4615 |
NN |
denotes |
function |
T8379 |
4616-4617 |
-LRB- |
denotes |
[ |
T8380 |
4617-4619 |
CD |
denotes |
21 |
T8381 |
4619-4620 |
-RRB- |
denotes |
] |
T8382 |
4620-4622 |
, |
denotes |
, |
T8383 |
4622-4624 |
PRP |
denotes |
we |
T8384 |
4633-4635 |
TO |
denotes |
to |
T8385 |
4636-4648 |
VB |
denotes |
characterize |
T8386 |
4649-4653 |
DT |
denotes |
this |
T8387 |
4654-4663 |
NN |
denotes |
phenotype |
T8388 |
4664-4666 |
IN |
denotes |
in |
T8389 |
4667-4671 |
JJR |
denotes |
more |
T8390 |
4672-4678 |
NN |
denotes |
detail |
T8391 |
4678-4679 |
. |
denotes |
. |
T8392 |
4679-4796 |
sentence |
denotes |
For this purpose, we examined the status of heterochromatin organization between the two-cell and blastocyst stages. |
T8393 |
4680-4683 |
IN |
denotes |
For |
T8394 |
4701-4709 |
VBD |
denotes |
examined |
T8395 |
4684-4688 |
DT |
denotes |
this |
T8396 |
4689-4696 |
NN |
denotes |
purpose |
T8397 |
4696-4698 |
, |
denotes |
, |
T8398 |
4698-4700 |
PRP |
denotes |
we |
T8399 |
4710-4713 |
DT |
denotes |
the |
T8400 |
4714-4720 |
NN |
denotes |
status |
T8401 |
4721-4723 |
IN |
denotes |
of |
T8402 |
4724-4739 |
NN |
denotes |
heterochromatin |
T8403 |
4740-4752 |
NN |
denotes |
organization |
T8404 |
4753-4760 |
IN |
denotes |
between |
T8405 |
4761-4764 |
DT |
denotes |
the |
T8406 |
4789-4795 |
NNS |
denotes |
stages |
T8407 |
4765-4768 |
CD |
denotes |
two |
T8408 |
4769-4773 |
NN |
denotes |
cell |
T8409 |
4768-4769 |
HYPH |
denotes |
- |
T8410 |
4774-4777 |
CC |
denotes |
and |
T8411 |
4778-4788 |
NN |
denotes |
blastocyst |
T8412 |
4795-4796 |
. |
denotes |
. |
T8413 |
4796-4955 |
sentence |
denotes |
In two-cell stage wild-type embryos, DAPI staining is diffuse and fibrillar, with regions of higher density around the nucleolar precursor bodies (Figure 1E). |
T8414 |
4797-4799 |
IN |
denotes |
In |
T8415 |
4848-4850 |
VBZ |
denotes |
is |
T8416 |
4800-4803 |
CD |
denotes |
two |
T8417 |
4804-4808 |
NN |
denotes |
cell |
T8418 |
4803-4804 |
HYPH |
denotes |
- |
T8419 |
4809-4814 |
NN |
denotes |
stage |
T8420 |
4825-4832 |
NNS |
denotes |
embryos |
T8421 |
4815-4819 |
JJ |
denotes |
wild |
T8422 |
4820-4824 |
NN |
denotes |
type |
T8423 |
4819-4820 |
HYPH |
denotes |
- |
T8424 |
4832-4834 |
, |
denotes |
, |
T8425 |
4834-4838 |
NN |
denotes |
DAPI |
T8426 |
4839-4847 |
NN |
denotes |
staining |
T8427 |
4851-4858 |
JJ |
denotes |
diffuse |
T8428 |
4859-4862 |
CC |
denotes |
and |
T8429 |
4863-4872 |
JJ |
denotes |
fibrillar |
T8430 |
4872-4874 |
, |
denotes |
, |
T8431 |
4874-4878 |
IN |
denotes |
with |
T8432 |
4879-4886 |
NNS |
denotes |
regions |
T8433 |
4887-4889 |
IN |
denotes |
of |
T8434 |
4890-4896 |
JJR |
denotes |
higher |
T8435 |
4897-4904 |
NN |
denotes |
density |
T8436 |
4905-4911 |
IN |
denotes |
around |
T8437 |
4912-4915 |
DT |
denotes |
the |
T8438 |
4936-4942 |
NNS |
denotes |
bodies |
T8439 |
4916-4925 |
JJ |
denotes |
nucleolar |
T8440 |
4926-4935 |
NN |
denotes |
precursor |
T8441 |
4943-4944 |
-LRB- |
denotes |
( |
T8442 |
4951-4953 |
NN |
denotes |
1E |
T8443 |
4944-4950 |
NN |
denotes |
Figure |
T8444 |
4953-4954 |
-RRB- |
denotes |
) |
T8445 |
4954-4955 |
. |
denotes |
. |
T8446 |
4955-5093 |
sentence |
denotes |
Heterochromatin domains are progressively assembled into DAPI-dense foci between the four-cell and 32-cell blastocyst stages (Figure 1F). |
T8447 |
4956-4971 |
NN |
denotes |
Heterochromatin |
T8448 |
4972-4979 |
NNS |
denotes |
domains |
T8449 |
4998-5007 |
VBN |
denotes |
assembled |
T8450 |
4980-4983 |
VBP |
denotes |
are |
T8451 |
4984-4997 |
RB |
denotes |
progressively |
T8452 |
5008-5012 |
IN |
denotes |
into |
T8453 |
5013-5017 |
NN |
denotes |
DAPI |
T8454 |
5018-5023 |
JJ |
denotes |
dense |
T8455 |
5017-5018 |
HYPH |
denotes |
- |
T8456 |
5024-5028 |
NNS |
denotes |
foci |
T8457 |
5029-5036 |
IN |
denotes |
between |
T8458 |
5037-5040 |
DT |
denotes |
the |
T8459 |
5074-5080 |
NNS |
denotes |
stages |
T8460 |
5041-5045 |
CD |
denotes |
four |
T8461 |
5046-5050 |
NN |
denotes |
cell |
T8462 |
5045-5046 |
HYPH |
denotes |
- |
T8463 |
5051-5054 |
CC |
denotes |
and |
T8464 |
5055-5057 |
CD |
denotes |
32 |
T8465 |
5058-5062 |
NN |
denotes |
cell |
T8466 |
5057-5058 |
HYPH |
denotes |
- |
T8467 |
5063-5073 |
NN |
denotes |
blastocyst |
T8468 |
5081-5082 |
-LRB- |
denotes |
( |
T8469 |
5089-5091 |
NN |
denotes |
1F |
T8470 |
5082-5088 |
NN |
denotes |
Figure |
T8471 |
5091-5092 |
-RRB- |
denotes |
) |
T8472 |
5092-5093 |
. |
denotes |
. |
T8473 |
5093-5228 |
sentence |
denotes |
Localization of HP1α confirmed that the structures visualized by DAPI staining correspond to constitutive heterochromatin (Figure 1E). |
T8474 |
5094-5106 |
NN |
denotes |
Localization |
T8475 |
5115-5124 |
VBD |
denotes |
confirmed |
T8476 |
5107-5109 |
IN |
denotes |
of |
T8477 |
5110-5114 |
NN |
denotes |
HP1α |
T8478 |
5125-5129 |
IN |
denotes |
that |
T8479 |
5173-5183 |
VBP |
denotes |
correspond |
T8480 |
5130-5133 |
DT |
denotes |
the |
T8481 |
5134-5144 |
NNS |
denotes |
structures |
T8482 |
5145-5155 |
VBN |
denotes |
visualized |
T8483 |
5156-5158 |
IN |
denotes |
by |
T8484 |
5159-5163 |
NN |
denotes |
DAPI |
T8485 |
5164-5172 |
NN |
denotes |
staining |
T8486 |
5184-5186 |
IN |
denotes |
to |
T8487 |
5187-5199 |
JJ |
denotes |
constitutive |
T8488 |
5200-5215 |
NN |
denotes |
heterochromatin |
T8489 |
5216-5217 |
-LRB- |
denotes |
( |
T8490 |
5224-5226 |
NN |
denotes |
1E |
T8491 |
5217-5223 |
NN |
denotes |
Figure |
T8492 |
5226-5227 |
-RRB- |
denotes |
) |
T8493 |
5227-5228 |
. |
denotes |
. |
T8494 |
5228-5384 |
sentence |
denotes |
Thus, the nuclear organization of heterochromatin is dramatically modified during pre-implantation development, between the two-cell and blastocyst stages. |
T8495 |
5229-5233 |
RB |
denotes |
Thus |
T8496 |
5295-5303 |
VBN |
denotes |
modified |
T8497 |
5233-5235 |
, |
denotes |
, |
T8498 |
5235-5238 |
DT |
denotes |
the |
T8499 |
5247-5259 |
NN |
denotes |
organization |
T8500 |
5239-5246 |
JJ |
denotes |
nuclear |
T8501 |
5260-5262 |
IN |
denotes |
of |
T8502 |
5263-5278 |
NN |
denotes |
heterochromatin |
T8503 |
5279-5281 |
VBZ |
denotes |
is |
T8504 |
5282-5294 |
RB |
denotes |
dramatically |
T8505 |
5304-5310 |
IN |
denotes |
during |
T8506 |
5311-5327 |
JJ |
denotes |
pre-implantation |
T8507 |
5328-5339 |
NN |
denotes |
development |
T8508 |
5339-5341 |
, |
denotes |
, |
T8509 |
5341-5348 |
IN |
denotes |
between |
T8510 |
5349-5352 |
DT |
denotes |
the |
T8511 |
5377-5383 |
NNS |
denotes |
stages |
T8512 |
5353-5356 |
CD |
denotes |
two |
T8513 |
5357-5361 |
NN |
denotes |
cell |
T8514 |
5356-5357 |
HYPH |
denotes |
- |
T8515 |
5362-5365 |
CC |
denotes |
and |
T8516 |
5366-5376 |
NN |
denotes |
blastocyst |
T8517 |
5383-5384 |
. |
denotes |
. |
T8518 |
5384-5519 |
sentence |
denotes |
In Chaf1a −/− E4 embryos, which are arrested between the eight- and 16-cell stage, DAPI-dense foci were barely detectable (Figure 1D). |
T8519 |
5385-5387 |
IN |
denotes |
In |
T8520 |
5484-5488 |
VBD |
denotes |
were |
T8521 |
5388-5394 |
NN |
denotes |
Chaf1a |
T8522 |
5402-5409 |
NNS |
denotes |
embryos |
T8523 |
5395-5396 |
SYM |
denotes |
− |
T8524 |
5396-5397 |
HYPH |
denotes |
/ |
T8525 |
5397-5398 |
SYM |
denotes |
− |
T8526 |
5399-5401 |
NN |
denotes |
E4 |
T8527 |
5409-5411 |
, |
denotes |
, |
T8528 |
5411-5416 |
WDT |
denotes |
which |
T8529 |
5421-5429 |
VBN |
denotes |
arrested |
T8530 |
5417-5420 |
VBP |
denotes |
are |
T8531 |
5430-5437 |
IN |
denotes |
between |
T8532 |
5438-5441 |
DT |
denotes |
the |
T8533 |
5461-5466 |
NN |
denotes |
stage |
T8534 |
5442-5447 |
CD |
denotes |
eight |
T8535 |
5456-5460 |
NN |
denotes |
cell |
T8536 |
5447-5448 |
HYPH |
denotes |
- |
T8537 |
5449-5452 |
CC |
denotes |
and |
T8539 |
5455-5456 |
HYPH |
denotes |
- |
T8540 |
5466-5468 |
, |
denotes |
, |
T8541 |
5468-5472 |
NN |
denotes |
DAPI |
T8542 |
5473-5478 |
JJ |
denotes |
dense |
T8543 |
5472-5473 |
HYPH |
denotes |
- |
T8544 |
5479-5483 |
NNS |
denotes |
foci |
T8545 |
5489-5495 |
RB |
denotes |
barely |
T8546 |
5496-5506 |
JJ |
denotes |
detectable |
T8547 |
5507-5508 |
-LRB- |
denotes |
( |
T8548 |
5515-5517 |
NN |
denotes |
1D |
T8549 |
5508-5514 |
NN |
denotes |
Figure |
T8550 |
5517-5518 |
-RRB- |
denotes |
) |
T8551 |
5518-5519 |
. |
denotes |
. |
T8552 |
5519-5673 |
sentence |
denotes |
Instead, DAPI staining was diffuse within the nucleus, with regions of higher density around the nucleoli and at the periphery of the nuclei (Figure 1D). |
T8553 |
5520-5527 |
RB |
denotes |
Instead |
T8554 |
5543-5546 |
VBD |
denotes |
was |
T8555 |
5527-5529 |
, |
denotes |
, |
T8556 |
5529-5533 |
NN |
denotes |
DAPI |
T8557 |
5534-5542 |
NN |
denotes |
staining |
T8558 |
5547-5554 |
JJ |
denotes |
diffuse |
T8559 |
5555-5561 |
IN |
denotes |
within |
T8560 |
5562-5565 |
DT |
denotes |
the |
T8561 |
5566-5573 |
NN |
denotes |
nucleus |
T8562 |
5573-5575 |
, |
denotes |
, |
T8563 |
5575-5579 |
IN |
denotes |
with |
T8564 |
5580-5587 |
NNS |
denotes |
regions |
T8565 |
5588-5590 |
IN |
denotes |
of |
T8566 |
5591-5597 |
JJR |
denotes |
higher |
T8567 |
5598-5605 |
NN |
denotes |
density |
T8568 |
5606-5612 |
IN |
denotes |
around |
T8569 |
5613-5616 |
DT |
denotes |
the |
T8570 |
5617-5625 |
NNS |
denotes |
nucleoli |
T8571 |
5626-5629 |
CC |
denotes |
and |
T8572 |
5630-5632 |
IN |
denotes |
at |
T8573 |
5633-5636 |
DT |
denotes |
the |
T8574 |
5637-5646 |
NN |
denotes |
periphery |
T8575 |
5647-5649 |
IN |
denotes |
of |
T8576 |
5650-5653 |
DT |
denotes |
the |
T8577 |
5654-5660 |
NNS |
denotes |
nuclei |
T8578 |
5661-5662 |
-LRB- |
denotes |
( |
T8579 |
5669-5671 |
NN |
denotes |
1D |
T8580 |
5662-5668 |
NN |
denotes |
Figure |
T8581 |
5671-5672 |
-RRB- |
denotes |
) |
T8582 |
5672-5673 |
. |
denotes |
. |
T8583 |
5673-5824 |
sentence |
denotes |
Localization of HP1α showed a diffuse pattern similar to the DAPI, with some enrichment at the nuclear periphery, and around the nucleoli (Figure 1D). |
T8584 |
5674-5686 |
NN |
denotes |
Localization |
T8585 |
5695-5701 |
VBD |
denotes |
showed |
T8586 |
5687-5689 |
IN |
denotes |
of |
T8587 |
5690-5694 |
NN |
denotes |
HP1α |
T8588 |
5702-5703 |
DT |
denotes |
a |
T8589 |
5712-5719 |
NN |
denotes |
pattern |
T8590 |
5704-5711 |
JJ |
denotes |
diffuse |
T8591 |
5720-5727 |
JJ |
denotes |
similar |
T8592 |
5728-5730 |
IN |
denotes |
to |
T8593 |
5731-5734 |
DT |
denotes |
the |
T8594 |
5735-5739 |
NN |
denotes |
DAPI |
T8595 |
5739-5741 |
, |
denotes |
, |
T8596 |
5741-5745 |
IN |
denotes |
with |
T8597 |
5746-5750 |
DT |
denotes |
some |
T8598 |
5751-5761 |
NN |
denotes |
enrichment |
T8599 |
5762-5764 |
IN |
denotes |
at |
T8600 |
5765-5768 |
DT |
denotes |
the |
T8601 |
5777-5786 |
NN |
denotes |
periphery |
T8602 |
5769-5776 |
JJ |
denotes |
nuclear |
T8603 |
5786-5788 |
, |
denotes |
, |
T8604 |
5788-5791 |
CC |
denotes |
and |
T8605 |
5792-5798 |
IN |
denotes |
around |
T8606 |
5799-5802 |
DT |
denotes |
the |
T8607 |
5803-5811 |
NNS |
denotes |
nucleoli |
T8608 |
5812-5813 |
-LRB- |
denotes |
( |
T8609 |
5820-5822 |
NN |
denotes |
1D |
T8610 |
5813-5819 |
NN |
denotes |
Figure |
T8611 |
5822-5823 |
-RRB- |
denotes |
) |
T8612 |
5823-5824 |
. |
denotes |
. |
T8613 |
5824-6022 |
sentence |
denotes |
This abnormal organization of heterochromatin in Chaf1a −/− embryos is reminiscent of the heterochromatin organization found in two- to four-cell stage wild-type embryos (compare Figure 1D and 1F). |
T8614 |
5825-5829 |
DT |
denotes |
This |
T8615 |
5839-5851 |
NN |
denotes |
organization |
T8616 |
5830-5838 |
JJ |
denotes |
abnormal |
T8617 |
5893-5895 |
VBZ |
denotes |
is |
T8618 |
5852-5854 |
IN |
denotes |
of |
T8619 |
5855-5870 |
NN |
denotes |
heterochromatin |
T8620 |
5871-5873 |
IN |
denotes |
in |
T8621 |
5874-5880 |
NN |
denotes |
Chaf1a |
T8622 |
5885-5892 |
NNS |
denotes |
embryos |
T8623 |
5881-5882 |
SYM |
denotes |
− |
T8624 |
5882-5883 |
HYPH |
denotes |
/ |
T8625 |
5883-5884 |
SYM |
denotes |
− |
T8626 |
5896-5907 |
JJ |
denotes |
reminiscent |
T8627 |
5908-5910 |
IN |
denotes |
of |
T8628 |
5911-5914 |
DT |
denotes |
the |
T8629 |
5931-5943 |
NN |
denotes |
organization |
T8630 |
5915-5930 |
NN |
denotes |
heterochromatin |
T8631 |
5944-5949 |
VBN |
denotes |
found |
T8632 |
5950-5952 |
IN |
denotes |
in |
T8633 |
5953-5956 |
CD |
denotes |
two |
T8634 |
5961-5965 |
CD |
denotes |
four |
T8635 |
5956-5957 |
HYPH |
denotes |
- |
T8636 |
5958-5960 |
IN |
denotes |
to |
T8637 |
5966-5970 |
NN |
denotes |
cell |
T8638 |
5965-5966 |
HYPH |
denotes |
- |
T8639 |
5971-5976 |
NN |
denotes |
stage |
T8640 |
5987-5994 |
NNS |
denotes |
embryos |
T8641 |
5977-5981 |
JJ |
denotes |
wild |
T8642 |
5982-5986 |
NN |
denotes |
type |
T8643 |
5981-5982 |
HYPH |
denotes |
- |
T8644 |
5995-5996 |
-LRB- |
denotes |
( |
T8645 |
5996-6003 |
VB |
denotes |
compare |
T8646 |
6004-6010 |
NN |
denotes |
Figure |
T8647 |
6011-6013 |
NN |
denotes |
1D |
T8648 |
6014-6017 |
CC |
denotes |
and |
T8649 |
6018-6020 |
NN |
denotes |
1F |
T8650 |
6020-6021 |
-RRB- |
denotes |
) |
T8651 |
6021-6022 |
. |
denotes |
. |
T8652 |
6022-6226 |
sentence |
denotes |
These data evidence a key role for p150CAF-1 during pre-implantation development, and reveal that this protein is required for the proper 3-D organization of heterochromatin within embryonic cell nuclei. |
T8653 |
6023-6028 |
DT |
denotes |
These |
T8654 |
6029-6033 |
NNS |
denotes |
data |
T8655 |
6034-6042 |
VBP |
denotes |
evidence |
T8656 |
6043-6044 |
DT |
denotes |
a |
T8657 |
6049-6053 |
NN |
denotes |
role |
T8658 |
6045-6048 |
JJ |
denotes |
key |
T8659 |
6054-6057 |
IN |
denotes |
for |
T8660 |
6058-6065 |
NN |
denotes |
p150CAF |
T8661 |
6065-6066 |
HYPH |
denotes |
- |
T8662 |
6066-6067 |
CD |
denotes |
1 |
T8663 |
6068-6074 |
IN |
denotes |
during |
T8664 |
6075-6091 |
JJ |
denotes |
pre-implantation |
T8665 |
6092-6103 |
NN |
denotes |
development |
T8666 |
6103-6105 |
, |
denotes |
, |
T8667 |
6105-6108 |
CC |
denotes |
and |
T8668 |
6109-6115 |
VBP |
denotes |
reveal |
T8669 |
6116-6120 |
IN |
denotes |
that |
T8670 |
6137-6145 |
VBN |
denotes |
required |
T8671 |
6121-6125 |
DT |
denotes |
this |
T8672 |
6126-6133 |
NN |
denotes |
protein |
T8673 |
6134-6136 |
VBZ |
denotes |
is |
T8674 |
6146-6149 |
IN |
denotes |
for |
T8675 |
6150-6153 |
DT |
denotes |
the |
T8676 |
6165-6177 |
NN |
denotes |
organization |
T8677 |
6154-6160 |
JJ |
denotes |
proper |
T8678 |
6161-6162 |
CD |
denotes |
3 |
T8679 |
6163-6164 |
NN |
denotes |
D |
T8680 |
6162-6163 |
HYPH |
denotes |
- |
T8681 |
6178-6180 |
IN |
denotes |
of |
T8682 |
6181-6196 |
NN |
denotes |
heterochromatin |
T8683 |
6197-6203 |
IN |
denotes |
within |
T8684 |
6204-6213 |
JJ |
denotes |
embryonic |
T8685 |
6219-6225 |
NNS |
denotes |
nuclei |
T8686 |
6214-6218 |
NN |
denotes |
cell |
T8687 |
6225-6226 |
. |
denotes |
. |
T8688 |
6226-6375 |
sentence |
denotes |
Next, we wondered whether a similar requirement for CAF-1 could also be observed in ES cells, which are derived from the blastocyst inner cell mass. |
T8689 |
6227-6231 |
RB |
denotes |
Next |
T8690 |
6236-6244 |
VBD |
denotes |
wondered |
T8691 |
6231-6233 |
, |
denotes |
, |
T8692 |
6233-6235 |
PRP |
denotes |
we |
T8693 |
6245-6252 |
IN |
denotes |
whether |
T8694 |
6299-6307 |
VBN |
denotes |
observed |
T8695 |
6253-6254 |
DT |
denotes |
a |
T8696 |
6263-6274 |
NN |
denotes |
requirement |
T8697 |
6255-6262 |
JJ |
denotes |
similar |
T8698 |
6275-6278 |
IN |
denotes |
for |
T8699 |
6279-6282 |
NN |
denotes |
CAF |
T8700 |
6282-6283 |
HYPH |
denotes |
- |
T8701 |
6283-6284 |
CD |
denotes |
1 |
T8702 |
6285-6290 |
MD |
denotes |
could |
T8703 |
6291-6295 |
RB |
denotes |
also |
T8704 |
6296-6298 |
VB |
denotes |
be |
T8705 |
6308-6310 |
IN |
denotes |
in |
T8706 |
6311-6313 |
NN |
denotes |
ES |
T8707 |
6314-6319 |
NNS |
denotes |
cells |
T8708 |
6319-6321 |
, |
denotes |
, |
T8709 |
6321-6326 |
WDT |
denotes |
which |
T8710 |
6331-6338 |
VBN |
denotes |
derived |
T8711 |
6327-6330 |
VBP |
denotes |
are |
T8712 |
6339-6343 |
IN |
denotes |
from |
T8713 |
6344-6347 |
DT |
denotes |
the |
T8714 |
6370-6374 |
NN |
denotes |
mass |
T8715 |
6348-6358 |
NN |
denotes |
blastocyst |
T8716 |
6359-6364 |
JJ |
denotes |
inner |
T8717 |
6365-6369 |
NN |
denotes |
cell |
T8718 |
6374-6375 |
. |
denotes |
. |
T8719 |
6375-6551 |
sentence |
denotes |
Given the early developmental arrest observed in Chaf1a −/− embryos, such cells could not be derived directly from null embryos, and we thus used an RNAi strategy (Figure 2A). |
T8720 |
6376-6381 |
VBN |
denotes |
Given |
T8721 |
6469-6476 |
VBN |
denotes |
derived |
T8722 |
6382-6385 |
DT |
denotes |
the |
T8723 |
6406-6412 |
NN |
denotes |
arrest |
T8724 |
6386-6391 |
JJ |
denotes |
early |
T8725 |
6392-6405 |
JJ |
denotes |
developmental |
T8726 |
6413-6421 |
VBN |
denotes |
observed |
T8727 |
6422-6424 |
IN |
denotes |
in |
T8728 |
6425-6431 |
NN |
denotes |
Chaf1a |
T8729 |
6436-6443 |
NNS |
denotes |
embryos |
T8730 |
6432-6433 |
SYM |
denotes |
− |
T8731 |
6433-6434 |
HYPH |
denotes |
/ |
T8732 |
6434-6435 |
SYM |
denotes |
− |
T8733 |
6443-6445 |
, |
denotes |
, |
T8734 |
6445-6449 |
JJ |
denotes |
such |
T8735 |
6450-6455 |
NNS |
denotes |
cells |
T8736 |
6456-6461 |
MD |
denotes |
could |
T8737 |
6462-6465 |
RB |
denotes |
not |
T8738 |
6466-6468 |
VB |
denotes |
be |
T8739 |
6477-6485 |
RB |
denotes |
directly |
T8740 |
6486-6490 |
IN |
denotes |
from |
T8741 |
6491-6495 |
JJ |
denotes |
null |
T8742 |
6496-6503 |
NNS |
denotes |
embryos |
T8743 |
6503-6505 |
, |
denotes |
, |
T8744 |
6505-6508 |
CC |
denotes |
and |
T8745 |
6509-6511 |
PRP |
denotes |
we |
T8746 |
6517-6521 |
VBD |
denotes |
used |
T8747 |
6512-6516 |
RB |
denotes |
thus |
T8748 |
6522-6524 |
DT |
denotes |
an |
T8749 |
6530-6538 |
NN |
denotes |
strategy |
T8750 |
6525-6529 |
NN |
denotes |
RNAi |
T8751 |
6539-6540 |
-LRB- |
denotes |
( |
T8752 |
6547-6549 |
NN |
denotes |
2A |
T8753 |
6540-6546 |
NN |
denotes |
Figure |
T8754 |
6549-6550 |
-RRB- |
denotes |
) |
T8755 |
6550-6551 |
. |
denotes |
. |
T8756 |
6551-6631 |
sentence |
denotes |
p150CAF-1 knockdown in ES cells was quantified by Western blot analysis and IF. |
T8757 |
6552-6559 |
NN |
denotes |
p150CAF |
T8758 |
6562-6571 |
NN |
denotes |
knockdown |
T8759 |
6559-6560 |
HYPH |
denotes |
- |
T8760 |
6560-6561 |
CD |
denotes |
1 |
T8761 |
6588-6598 |
VBN |
denotes |
quantified |
T8762 |
6572-6574 |
IN |
denotes |
in |
T8763 |
6575-6577 |
NN |
denotes |
ES |
T8764 |
6578-6583 |
NNS |
denotes |
cells |
T8765 |
6584-6587 |
VBD |
denotes |
was |
T8766 |
6599-6601 |
IN |
denotes |
by |
T8767 |
6602-6609 |
NNP |
denotes |
Western |
T8768 |
6610-6614 |
NN |
denotes |
blot |
T8769 |
6615-6623 |
NN |
denotes |
analysis |
T8770 |
6624-6627 |
CC |
denotes |
and |
T8771 |
6628-6630 |
NN |
denotes |
IF |
T8772 |
6630-6631 |
. |
denotes |
. |
T8773 |
6631-6644 |
sentence |
denotes |
(Figure S1). |
T8774 |
6632-6633 |
-LRB- |
denotes |
( |
T8775 |
6640-6642 |
NN |
denotes |
S1 |
T8776 |
6633-6639 |
NN |
denotes |
Figure |
T8777 |
6642-6643 |
-RRB- |
denotes |
) |
T8778 |
6643-6644 |
. |
denotes |
. |
T8779 |
6644-6748 |
sentence |
denotes |
p150CAF-1 RNAi-depleted ES cells displayed a phenotype very similar to the cells of Chaf1a −/− E4 mice. |
T8780 |
6645-6652 |
NN |
denotes |
p150CAF |
T8781 |
6672-6677 |
NNS |
denotes |
cells |
T8782 |
6652-6653 |
HYPH |
denotes |
- |
T8783 |
6653-6654 |
CD |
denotes |
1 |
T8784 |
6655-6659 |
NN |
denotes |
RNAi |
T8785 |
6660-6668 |
VBN |
denotes |
depleted |
T8786 |
6659-6660 |
HYPH |
denotes |
- |
T8787 |
6669-6671 |
NN |
denotes |
ES |
T8788 |
6678-6687 |
VBD |
denotes |
displayed |
T8789 |
6688-6689 |
DT |
denotes |
a |
T8790 |
6690-6699 |
NN |
denotes |
phenotype |
T8791 |
6700-6704 |
RB |
denotes |
very |
T8792 |
6705-6712 |
JJ |
denotes |
similar |
T8793 |
6713-6715 |
IN |
denotes |
to |
T8794 |
6716-6719 |
DT |
denotes |
the |
T8795 |
6720-6725 |
NNS |
denotes |
cells |
T8796 |
6726-6728 |
IN |
denotes |
of |
T8797 |
6729-6735 |
NN |
denotes |
Chaf1a |
T8798 |
6743-6747 |
NNS |
denotes |
mice |
T8799 |
6736-6737 |
SYM |
denotes |
− |
T8800 |
6737-6738 |
HYPH |
denotes |
/ |
T8801 |
6738-6739 |
SYM |
denotes |
− |
T8802 |
6740-6742 |
NN |
denotes |
E4 |
T8803 |
6747-6748 |
. |
denotes |
. |
T8804 |
6748-6891 |
sentence |
denotes |
DAPI-dense foci were lost, and we observed diffuse HP1α and DAPI staining around the nucleoli and at the periphery of the nucleus (Figure 2B). |
T8805 |
6749-6753 |
NN |
denotes |
DAPI |
T8806 |
6754-6759 |
JJ |
denotes |
dense |
T8807 |
6753-6754 |
HYPH |
denotes |
- |
T8808 |
6760-6764 |
NNS |
denotes |
foci |
T8809 |
6770-6774 |
VBN |
denotes |
lost |
T8810 |
6765-6769 |
VBD |
denotes |
were |
T8811 |
6774-6776 |
, |
denotes |
, |
T8812 |
6776-6779 |
CC |
denotes |
and |
T8813 |
6780-6782 |
PRP |
denotes |
we |
T8814 |
6783-6791 |
VBD |
denotes |
observed |
T8815 |
6792-6799 |
JJ |
denotes |
diffuse |
T8816 |
6814-6822 |
NN |
denotes |
staining |
T8817 |
6800-6804 |
NN |
denotes |
HP1α |
T8818 |
6805-6808 |
CC |
denotes |
and |
T8819 |
6809-6813 |
NN |
denotes |
DAPI |
T8820 |
6823-6829 |
IN |
denotes |
around |
T8821 |
6830-6833 |
DT |
denotes |
the |
T8822 |
6834-6842 |
NNS |
denotes |
nucleoli |
T8823 |
6843-6846 |
CC |
denotes |
and |
T8824 |
6847-6849 |
IN |
denotes |
at |
T8825 |
6850-6853 |
DT |
denotes |
the |
T8826 |
6854-6863 |
NN |
denotes |
periphery |
T8827 |
6864-6866 |
IN |
denotes |
of |
T8828 |
6867-6870 |
DT |
denotes |
the |
T8829 |
6871-6878 |
NN |
denotes |
nucleus |
T8830 |
6879-6880 |
-LRB- |
denotes |
( |
T8831 |
6887-6889 |
NN |
denotes |
2B |
T8832 |
6880-6886 |
NN |
denotes |
Figure |
T8833 |
6889-6890 |
-RRB- |
denotes |
) |
T8834 |
6890-6891 |
. |
denotes |
. |
T8835 |
6891-7049 |
sentence |
denotes |
This result indicates that p150CAF-1 is essential for nuclear organization of heterochromatin in ES cells in a similar way to early pre-implantation embryos. |
T8836 |
6892-6896 |
DT |
denotes |
This |
T8837 |
6897-6903 |
NN |
denotes |
result |
T8838 |
6904-6913 |
VBZ |
denotes |
indicates |
T8839 |
6914-6918 |
IN |
denotes |
that |
T8840 |
6929-6931 |
VBZ |
denotes |
is |
T8841 |
6919-6926 |
NN |
denotes |
p150CAF |
T8842 |
6926-6927 |
HYPH |
denotes |
- |
T8843 |
6927-6928 |
CD |
denotes |
1 |
T8844 |
6932-6941 |
JJ |
denotes |
essential |
T8845 |
6942-6945 |
IN |
denotes |
for |
T8846 |
6946-6953 |
JJ |
denotes |
nuclear |
T8847 |
6954-6966 |
NN |
denotes |
organization |
T8848 |
6967-6969 |
IN |
denotes |
of |
T8849 |
6970-6985 |
NN |
denotes |
heterochromatin |
T8850 |
6986-6988 |
IN |
denotes |
in |
T8851 |
6989-6991 |
NN |
denotes |
ES |
T8852 |
6992-6997 |
NNS |
denotes |
cells |
T8853 |
6998-7000 |
IN |
denotes |
in |
T8854 |
7001-7002 |
DT |
denotes |
a |
T8855 |
7011-7014 |
NN |
denotes |
way |
T8856 |
7003-7010 |
JJ |
denotes |
similar |
T8857 |
7015-7017 |
IN |
denotes |
to |
T8858 |
7018-7023 |
JJ |
denotes |
early |
T8859 |
7041-7048 |
NNS |
denotes |
embryos |
T8860 |
7024-7040 |
JJ |
denotes |
pre-implantation |
T8861 |
7048-7049 |
. |
denotes |
. |
T8862 |
7049-7222 |
sentence |
denotes |
Surprisingly, we did not observe this severe alteration in heterochromatin organization in primary mouse embryonic fibroblasts (MEFs) following p150CAF-1 depletion by RNAi. |
T8863 |
7050-7062 |
RB |
denotes |
Surprisingly |
T8864 |
7075-7082 |
VB |
denotes |
observe |
T8865 |
7062-7064 |
, |
denotes |
, |
T8866 |
7064-7066 |
PRP |
denotes |
we |
T8867 |
7067-7070 |
VBD |
denotes |
did |
T8868 |
7071-7074 |
RB |
denotes |
not |
T8869 |
7083-7087 |
DT |
denotes |
this |
T8870 |
7095-7105 |
NN |
denotes |
alteration |
T8871 |
7088-7094 |
JJ |
denotes |
severe |
T8872 |
7106-7108 |
IN |
denotes |
in |
T8873 |
7109-7124 |
NN |
denotes |
heterochromatin |
T8874 |
7125-7137 |
NN |
denotes |
organization |
T8875 |
7138-7140 |
IN |
denotes |
in |
T8876 |
7141-7148 |
JJ |
denotes |
primary |
T8877 |
7165-7176 |
NNS |
denotes |
fibroblasts |
T8878 |
7149-7154 |
NN |
denotes |
mouse |
T8879 |
7155-7164 |
JJ |
denotes |
embryonic |
T8880 |
7177-7178 |
-LRB- |
denotes |
( |
T8881 |
7178-7182 |
NNS |
denotes |
MEFs |
T8882 |
7182-7183 |
-RRB- |
denotes |
) |
T8883 |
7184-7193 |
VBG |
denotes |
following |
T8884 |
7194-7201 |
NN |
denotes |
p150CAF |
T8885 |
7204-7213 |
NN |
denotes |
depletion |
T8886 |
7201-7202 |
HYPH |
denotes |
- |
T8887 |
7202-7203 |
CD |
denotes |
1 |
T8888 |
7214-7216 |
IN |
denotes |
by |
T8889 |
7217-7221 |
NN |
denotes |
RNAi |
T8890 |
7221-7222 |
. |
denotes |
. |
T8891 |
7222-7405 |
sentence |
denotes |
p150CAF-1 depletion in MEFs resulted in a strong inhibition of cell proliferation (unpublished data), but did not alter the clustering of heterochromatin domains (Figures 2C and S2). |
T8892 |
7223-7230 |
NN |
denotes |
p150CAF |
T8893 |
7233-7242 |
NN |
denotes |
depletion |
T8894 |
7230-7231 |
HYPH |
denotes |
- |
T8895 |
7231-7232 |
CD |
denotes |
1 |
T8896 |
7251-7259 |
VBD |
denotes |
resulted |
T8897 |
7243-7245 |
IN |
denotes |
in |
T8898 |
7246-7250 |
NNS |
denotes |
MEFs |
T8899 |
7260-7262 |
IN |
denotes |
in |
T8900 |
7263-7264 |
DT |
denotes |
a |
T8901 |
7272-7282 |
NN |
denotes |
inhibition |
T8902 |
7265-7271 |
JJ |
denotes |
strong |
T8903 |
7283-7285 |
IN |
denotes |
of |
T8904 |
7286-7290 |
NN |
denotes |
cell |
T8905 |
7291-7304 |
NN |
denotes |
proliferation |
T8906 |
7305-7306 |
-LRB- |
denotes |
( |
T8907 |
7318-7322 |
NNS |
denotes |
data |
T8908 |
7306-7317 |
JJ |
denotes |
unpublished |
T8909 |
7322-7323 |
-RRB- |
denotes |
) |
T8910 |
7323-7325 |
, |
denotes |
, |
T8911 |
7325-7328 |
CC |
denotes |
but |
T8912 |
7329-7332 |
VBD |
denotes |
did |
T8913 |
7337-7342 |
VB |
denotes |
alter |
T8914 |
7333-7336 |
RB |
denotes |
not |
T8915 |
7343-7346 |
DT |
denotes |
the |
T8916 |
7347-7357 |
NN |
denotes |
clustering |
T8917 |
7358-7360 |
IN |
denotes |
of |
T8918 |
7361-7376 |
NN |
denotes |
heterochromatin |
T8919 |
7377-7384 |
NNS |
denotes |
domains |
T8920 |
7385-7386 |
-LRB- |
denotes |
( |
T8921 |
7394-7396 |
NN |
denotes |
2C |
T8922 |
7386-7393 |
NNS |
denotes |
Figures |
T8923 |
7397-7400 |
CC |
denotes |
and |
T8924 |
7401-7403 |
NN |
denotes |
S2 |
T8925 |
7403-7404 |
-RRB- |
denotes |
) |
T8926 |
7404-7405 |
. |
denotes |
. |
T8927 |
7405-7499 |
sentence |
denotes |
Similarly, p150CAF-1 depletion in 3T3 cells did not affect heterochromatin organization [16]. |
T8928 |
7406-7415 |
RB |
denotes |
Similarly |
T8929 |
7458-7464 |
VB |
denotes |
affect |
T8930 |
7415-7417 |
, |
denotes |
, |
T8931 |
7417-7424 |
NN |
denotes |
p150CAF |
T8932 |
7427-7436 |
NN |
denotes |
depletion |
T8933 |
7424-7425 |
HYPH |
denotes |
- |
T8934 |
7425-7426 |
CD |
denotes |
1 |
T8935 |
7437-7439 |
IN |
denotes |
in |
T8936 |
7440-7443 |
NN |
denotes |
3T3 |
T8937 |
7444-7449 |
NNS |
denotes |
cells |
T8938 |
7450-7453 |
VBD |
denotes |
did |
T8939 |
7454-7457 |
RB |
denotes |
not |
T8940 |
7465-7480 |
NN |
denotes |
heterochromatin |
T8941 |
7481-7493 |
NN |
denotes |
organization |
T8942 |
7494-7495 |
-LRB- |
denotes |
[ |
T8943 |
7495-7497 |
CD |
denotes |
16 |
T8944 |
7497-7498 |
-RRB- |
denotes |
] |
T8945 |
7498-7499 |
. |
denotes |
. |
T8946 |
7499-7589 |
sentence |
denotes |
These observations reveal a specific function for CAF-1 in the early embryo and ES cells. |
T8947 |
7500-7505 |
DT |
denotes |
These |
T8948 |
7506-7518 |
NNS |
denotes |
observations |
T8949 |
7519-7525 |
VBP |
denotes |
reveal |
T8950 |
7526-7527 |
DT |
denotes |
a |
T8951 |
7537-7545 |
NN |
denotes |
function |
T8952 |
7528-7536 |
JJ |
denotes |
specific |
T8953 |
7546-7549 |
IN |
denotes |
for |
T8954 |
7550-7553 |
NN |
denotes |
CAF |
T8955 |
7553-7554 |
HYPH |
denotes |
- |
T8956 |
7554-7555 |
CD |
denotes |
1 |
T8957 |
7556-7558 |
IN |
denotes |
in |
T8958 |
7559-7562 |
DT |
denotes |
the |
T8959 |
7569-7575 |
NN |
denotes |
embryo |
T8960 |
7563-7568 |
JJ |
denotes |
early |
T8961 |
7576-7579 |
CC |
denotes |
and |
T8962 |
7580-7582 |
NN |
denotes |
ES |
T8963 |
7583-7588 |
NNS |
denotes |
cells |
T8964 |
7588-7589 |
. |
denotes |
. |
T8965 |
7589-9072 |
sentence |
denotes |
Figure 2 Depletion of p150CAF-1 in ES Cells Results in a Severe Alteration of Heterochromatin Organization
(A) Strategy used to deplete p150CAF-1 by RNAi in ES cells. The siRNA expression vector includes a puromycin selection cassette (Puro), the mouse H1 promoter, and the siRNA encoding sequence. ES cells were kept under puromycin selection during 48 h following transfection.
(B) Abnormal heterochromatin organization in p150CAF-1-depleted ES cells. Immunodetection of p150CAF-1 (green) and HP1α (red) in ES cells transfected with control (cont) and p150CAF-1 siRNA. siRNA expression results in efficient p150CAF-1 depletion. The right-hand image shows the merge between HP1α fluorescence and DAPI-stained DNA in blue. Scale bar = 10 μm.
(C) Heterochromatin organization is not altered in p150CAF-1-depleted MEFs. Immunodetection of p150CAF-1 (green) and HP1α (red) in MEFs transfected with control (cont) or p150CAF-1 siRNA. The right-hand image shows the merge between HP1α fluorescence and DAPI-stained DNA in blue.
(D) PML bodies are not altered in p150CAF-1-depleted ES cells. Immunodetection of PML (red) in ES cells transfected with control (cont) or p150CAF-1 siRNA. The right-hand image shows the merge between PML fluorescence and DAPI-stained DNA in blue. We also wondered whether loss of p150CAF-1 in ES cells specifically affects the heterochromatin nuclear subcompartment, or whether it might result in a more global loss of nuclear organization and architecture. |
T8966 |
8862-8864 |
PRP |
denotes |
We |
T8967 |
8870-8878 |
VBD |
denotes |
wondered |
T8968 |
8865-8869 |
RB |
denotes |
also |
T8969 |
8879-8886 |
IN |
denotes |
whether |
T8970 |
8930-8937 |
VBZ |
denotes |
affects |
T8971 |
8887-8891 |
NN |
denotes |
loss |
T8972 |
8892-8894 |
IN |
denotes |
of |
T8973 |
8895-8902 |
NN |
denotes |
p150CAF |
T8974 |
8902-8903 |
HYPH |
denotes |
- |
T8975 |
8903-8904 |
CD |
denotes |
1 |
T8976 |
8905-8907 |
IN |
denotes |
in |
T8977 |
8908-8910 |
NN |
denotes |
ES |
T8978 |
8911-8916 |
NNS |
denotes |
cells |
T8979 |
8917-8929 |
RB |
denotes |
specifically |
T8980 |
8938-8941 |
DT |
denotes |
the |
T8981 |
8966-8980 |
NN |
denotes |
subcompartment |
T8982 |
8942-8957 |
NN |
denotes |
heterochromatin |
T8983 |
8958-8965 |
JJ |
denotes |
nuclear |
T8984 |
8980-8982 |
, |
denotes |
, |
T8985 |
8982-8984 |
CC |
denotes |
or |
T8986 |
8985-8992 |
IN |
denotes |
whether |
T8987 |
9002-9008 |
VB |
denotes |
result |
T8988 |
8993-8995 |
PRP |
denotes |
it |
T8989 |
8996-9001 |
MD |
denotes |
might |
T8990 |
9009-9011 |
IN |
denotes |
in |
T8991 |
9012-9013 |
DT |
denotes |
a |
T8992 |
9026-9030 |
NN |
denotes |
loss |
T8993 |
9014-9018 |
RBR |
denotes |
more |
T8994 |
9019-9025 |
JJ |
denotes |
global |
T8995 |
9031-9033 |
IN |
denotes |
of |
T8996 |
9034-9041 |
JJ |
denotes |
nuclear |
T8997 |
9042-9054 |
NN |
denotes |
organization |
T8998 |
9055-9058 |
CC |
denotes |
and |
T8999 |
9059-9071 |
NN |
denotes |
architecture |
T9000 |
9071-9072 |
. |
denotes |
. |
T9001 |
9072-9251 |
sentence |
denotes |
We used a specific antibody against the promyelocytic leukemia (PML) protein to study the fate of the PML nuclear bodies, which are well-characterized subnuclear structures [22]. |
T9002 |
9073-9075 |
PRP |
denotes |
We |
T9003 |
9076-9080 |
VBD |
denotes |
used |
T9004 |
9081-9082 |
DT |
denotes |
a |
T9005 |
9092-9100 |
NN |
denotes |
antibody |
T9006 |
9083-9091 |
JJ |
denotes |
specific |
T9007 |
9101-9108 |
IN |
denotes |
against |
T9008 |
9109-9112 |
DT |
denotes |
the |
T9009 |
9142-9149 |
NN |
denotes |
protein |
T9010 |
9113-9126 |
JJ |
denotes |
promyelocytic |
T9011 |
9127-9135 |
NN |
denotes |
leukemia |
T9012 |
9136-9137 |
-LRB- |
denotes |
( |
T9013 |
9137-9140 |
NN |
denotes |
PML |
T9014 |
9140-9141 |
-RRB- |
denotes |
) |
T9015 |
9150-9152 |
TO |
denotes |
to |
T9016 |
9153-9158 |
VB |
denotes |
study |
T9017 |
9159-9162 |
DT |
denotes |
the |
T9018 |
9163-9167 |
NN |
denotes |
fate |
T9019 |
9168-9170 |
IN |
denotes |
of |
T9020 |
9171-9174 |
DT |
denotes |
the |
T9021 |
9187-9193 |
NNS |
denotes |
bodies |
T9022 |
9175-9178 |
NN |
denotes |
PML |
T9023 |
9179-9186 |
JJ |
denotes |
nuclear |
T9024 |
9193-9195 |
, |
denotes |
, |
T9025 |
9195-9200 |
WDT |
denotes |
which |
T9026 |
9201-9204 |
VBP |
denotes |
are |
T9027 |
9205-9209 |
RB |
denotes |
well |
T9028 |
9210-9223 |
VBN |
denotes |
characterized |
T9029 |
9209-9210 |
HYPH |
denotes |
- |
T9030 |
9235-9245 |
NNS |
denotes |
structures |
T9031 |
9224-9234 |
JJ |
denotes |
subnuclear |
T9032 |
9246-9247 |
-LRB- |
denotes |
[ |
T9033 |
9247-9249 |
CD |
denotes |
22 |
T9034 |
9249-9250 |
-RRB- |
denotes |
] |
T9035 |
9250-9251 |
. |
denotes |
. |
T9036 |
9251-9412 |
sentence |
denotes |
We could not detect any significant difference in the distribution and aspect of PML nuclear bodies between control and p150CAF-1-depleted ES cells (Figure 2D). |
T9037 |
9252-9254 |
PRP |
denotes |
We |
T9038 |
9265-9271 |
VB |
denotes |
detect |
T9039 |
9255-9260 |
MD |
denotes |
could |
T9040 |
9261-9264 |
RB |
denotes |
not |
T9041 |
9272-9275 |
DT |
denotes |
any |
T9042 |
9288-9298 |
NN |
denotes |
difference |
T9043 |
9276-9287 |
JJ |
denotes |
significant |
T9044 |
9299-9301 |
IN |
denotes |
in |
T9045 |
9302-9305 |
DT |
denotes |
the |
T9046 |
9306-9318 |
NN |
denotes |
distribution |
T9047 |
9319-9322 |
CC |
denotes |
and |
T9048 |
9323-9329 |
NN |
denotes |
aspect |
T9049 |
9330-9332 |
IN |
denotes |
of |
T9050 |
9333-9336 |
NN |
denotes |
PML |
T9051 |
9345-9351 |
NNS |
denotes |
bodies |
T9052 |
9337-9344 |
JJ |
denotes |
nuclear |
T9053 |
9352-9359 |
IN |
denotes |
between |
T9054 |
9360-9367 |
NN |
denotes |
control |
T9055 |
9394-9399 |
NNS |
denotes |
cells |
T9056 |
9368-9371 |
CC |
denotes |
and |
T9057 |
9372-9379 |
NN |
denotes |
p150CAF |
T9058 |
9382-9390 |
VBN |
denotes |
depleted |
T9059 |
9379-9380 |
HYPH |
denotes |
- |
T9060 |
9380-9381 |
CD |
denotes |
1 |
T9061 |
9381-9382 |
HYPH |
denotes |
- |
T9062 |
9391-9393 |
NN |
denotes |
ES |
T9063 |
9400-9401 |
-LRB- |
denotes |
( |
T9064 |
9408-9410 |
NN |
denotes |
2D |
T9065 |
9401-9407 |
NN |
denotes |
Figure |
T9066 |
9410-9411 |
-RRB- |
denotes |
) |
T9067 |
9411-9412 |
. |
denotes |
. |
T9068 |
9412-9512 |
sentence |
denotes |
These data show that nuclear architecture is not globally altered following CAF-1 loss-of-function. |
T9069 |
9413-9418 |
DT |
denotes |
These |
T9070 |
9419-9423 |
NNS |
denotes |
data |
T9071 |
9424-9428 |
VBP |
denotes |
show |
T9072 |
9429-9433 |
IN |
denotes |
that |
T9073 |
9471-9478 |
VBN |
denotes |
altered |
T9074 |
9434-9441 |
JJ |
denotes |
nuclear |
T9075 |
9442-9454 |
NN |
denotes |
architecture |
T9076 |
9455-9457 |
VBZ |
denotes |
is |
T9077 |
9458-9461 |
RB |
denotes |
not |
T9078 |
9462-9470 |
RB |
denotes |
globally |
T9079 |
9479-9488 |
VBG |
denotes |
following |
T9080 |
9489-9492 |
NN |
denotes |
CAF |
T9081 |
9492-9493 |
HYPH |
denotes |
- |
T9082 |
9493-9494 |
CD |
denotes |
1 |
T9083 |
9495-9499 |
NN |
denotes |
loss |
T9084 |
9499-9500 |
HYPH |
denotes |
- |
T9085 |
9500-9502 |
IN |
denotes |
of |
T9086 |
9502-9503 |
HYPH |
denotes |
- |
T9087 |
9503-9511 |
NN |
denotes |
function |
T9088 |
9511-9512 |
. |
denotes |
. |
T9089 |
9512-9687 |
sentence |
denotes |
In addition, Western blot analysis revealed that CAF-1 depletion did not result in altered levels of chromatin architectural proteins such as histone H3 and HP1α (Figure S1). |
T9090 |
9513-9515 |
IN |
denotes |
In |
T9091 |
9548-9556 |
VBD |
denotes |
revealed |
T9092 |
9516-9524 |
NN |
denotes |
addition |
T9093 |
9524-9526 |
, |
denotes |
, |
T9094 |
9526-9533 |
NNP |
denotes |
Western |
T9095 |
9534-9538 |
NN |
denotes |
blot |
T9096 |
9539-9547 |
NN |
denotes |
analysis |
T9097 |
9557-9561 |
IN |
denotes |
that |
T9098 |
9586-9592 |
VB |
denotes |
result |
T9099 |
9562-9565 |
NN |
denotes |
CAF |
T9100 |
9568-9577 |
NN |
denotes |
depletion |
T9101 |
9565-9566 |
HYPH |
denotes |
- |
T9102 |
9566-9567 |
CD |
denotes |
1 |
T9103 |
9578-9581 |
VBD |
denotes |
did |
T9104 |
9582-9585 |
RB |
denotes |
not |
T9105 |
9593-9595 |
IN |
denotes |
in |
T9106 |
9596-9603 |
VBN |
denotes |
altered |
T9107 |
9604-9610 |
NNS |
denotes |
levels |
T9108 |
9611-9613 |
IN |
denotes |
of |
T9109 |
9614-9623 |
NN |
denotes |
chromatin |
T9110 |
9638-9646 |
NN |
denotes |
proteins |
T9111 |
9624-9637 |
JJ |
denotes |
architectural |
T9112 |
9647-9651 |
JJ |
denotes |
such |
T9113 |
9652-9654 |
IN |
denotes |
as |
T9114 |
9655-9662 |
NN |
denotes |
histone |
T9115 |
9663-9665 |
NN |
denotes |
H3 |
T9116 |
9666-9669 |
CC |
denotes |
and |
T9117 |
9670-9674 |
NN |
denotes |
HP1α |
T9118 |
9675-9676 |
-LRB- |
denotes |
( |
T9119 |
9683-9685 |
NN |
denotes |
S1 |
T9120 |
9676-9682 |
NN |
denotes |
Figure |
T9121 |
9685-9686 |
-RRB- |
denotes |
) |
T9122 |
9686-9687 |
. |
denotes |
. |
T9123 |
9687-9832 |
sentence |
denotes |
Thus, CAF-1 is specifically required in early embryos and in ES cells for the proper organization of the heterochromatin subnuclear compartment. |
T9124 |
9688-9692 |
RB |
denotes |
Thus |
T9125 |
9716-9724 |
VBN |
denotes |
required |
T9126 |
9692-9694 |
, |
denotes |
, |
T9127 |
9694-9697 |
NN |
denotes |
CAF |
T9128 |
9697-9698 |
HYPH |
denotes |
- |
T9129 |
9698-9699 |
CD |
denotes |
1 |
T9130 |
9700-9702 |
VBZ |
denotes |
is |
T9131 |
9703-9715 |
RB |
denotes |
specifically |
T9132 |
9725-9727 |
IN |
denotes |
in |
T9133 |
9728-9733 |
JJ |
denotes |
early |
T9134 |
9734-9741 |
NNS |
denotes |
embryos |
T9135 |
9742-9745 |
CC |
denotes |
and |
T9136 |
9746-9748 |
IN |
denotes |
in |
T9137 |
9749-9751 |
NN |
denotes |
ES |
T9138 |
9752-9757 |
NNS |
denotes |
cells |
T9139 |
9758-9761 |
IN |
denotes |
for |
T9140 |
9762-9765 |
DT |
denotes |
the |
T9141 |
9773-9785 |
NN |
denotes |
organization |
T9142 |
9766-9772 |
JJ |
denotes |
proper |
T9143 |
9786-9788 |
IN |
denotes |
of |
T9144 |
9789-9792 |
DT |
denotes |
the |
T9145 |
9820-9831 |
NN |
denotes |
compartment |
T9146 |
9793-9808 |
NN |
denotes |
heterochromatin |
T9147 |
9809-9819 |
JJ |
denotes |
subnuclear |
T9148 |
9831-9832 |
. |
denotes |
. |
T9149 |
9832-9958 |
sentence |
denotes |
Previous studies performed in mammalian cell lines showed that CAF-1 activity is required for S phase progression [16,23,24]. |
T9150 |
9833-9841 |
JJ |
denotes |
Previous |
T9151 |
9842-9849 |
NNS |
denotes |
studies |
T9152 |
9884-9890 |
VBD |
denotes |
showed |
T9153 |
9850-9859 |
VBN |
denotes |
performed |
T9154 |
9860-9862 |
IN |
denotes |
in |
T9155 |
9863-9872 |
JJ |
denotes |
mammalian |
T9156 |
9878-9883 |
NNS |
denotes |
lines |
T9157 |
9873-9877 |
NN |
denotes |
cell |
T9158 |
9891-9895 |
IN |
denotes |
that |
T9159 |
9914-9922 |
VBN |
denotes |
required |
T9160 |
9896-9899 |
NN |
denotes |
CAF |
T9161 |
9902-9910 |
NN |
denotes |
activity |
T9162 |
9899-9900 |
HYPH |
denotes |
- |
T9163 |
9900-9901 |
CD |
denotes |
1 |
T9164 |
9911-9913 |
VBZ |
denotes |
is |
T9165 |
9923-9926 |
IN |
denotes |
for |
T9166 |
9927-9928 |
NN |
denotes |
S |
T9167 |
9929-9934 |
NN |
denotes |
phase |
T9168 |
9935-9946 |
NN |
denotes |
progression |
T9169 |
9947-9948 |
-LRB- |
denotes |
[ |
T9170 |
9954-9956 |
CD |
denotes |
24 |
T9171 |
9948-9950 |
CD |
denotes |
16 |
T9172 |
9950-9951 |
, |
denotes |
, |
T9173 |
9951-9953 |
CD |
denotes |
23 |
T9174 |
9953-9954 |
, |
denotes |
, |
T9175 |
9956-9957 |
-RRB- |
denotes |
] |
T9176 |
9957-9958 |
. |
denotes |
. |
T9177 |
9958-10334 |
sentence |
denotes |
We show here that 3 d after transfection of the RNAi vector, p150CAF-1-depleted cells (identified by a complete absence of CAF-1 IF signal) appear still active for replication, as revealed by incorporation of the thymidine analog bromodeoxyuridine (BrdU) (Figure 3A), the PCNA (proliferating cell nuclear antigen) pattern (Figure 3B), and flow cytometry analysis (Figure 3C). |
T9178 |
9959-9961 |
PRP |
denotes |
We |
T9179 |
9962-9966 |
VBP |
denotes |
show |
T9180 |
9967-9971 |
RB |
denotes |
here |
T9181 |
9972-9976 |
IN |
denotes |
that |
T9182 |
10099-10105 |
VBP |
denotes |
appear |
T9183 |
9977-9978 |
CD |
denotes |
3 |
T9184 |
9979-9980 |
NN |
denotes |
d |
T9185 |
9981-9986 |
IN |
denotes |
after |
T9186 |
9987-9999 |
NN |
denotes |
transfection |
T9187 |
10000-10002 |
IN |
denotes |
of |
T9188 |
10003-10006 |
DT |
denotes |
the |
T9189 |
10012-10018 |
NN |
denotes |
vector |
T9190 |
10007-10011 |
NN |
denotes |
RNAi |
T9191 |
10018-10020 |
, |
denotes |
, |
T9192 |
10020-10027 |
NN |
denotes |
p150CAF |
T9193 |
10030-10038 |
VBN |
denotes |
depleted |
T9194 |
10027-10028 |
HYPH |
denotes |
- |
T9195 |
10028-10029 |
CD |
denotes |
1 |
T9196 |
10029-10030 |
HYPH |
denotes |
- |
T9197 |
10039-10044 |
NNS |
denotes |
cells |
T9198 |
10045-10046 |
-LRB- |
denotes |
( |
T9199 |
10046-10056 |
VBN |
denotes |
identified |
T9200 |
10057-10059 |
IN |
denotes |
by |
T9201 |
10060-10061 |
DT |
denotes |
a |
T9202 |
10071-10078 |
NN |
denotes |
absence |
T9203 |
10062-10070 |
JJ |
denotes |
complete |
T9204 |
10079-10081 |
IN |
denotes |
of |
T9205 |
10082-10085 |
NN |
denotes |
CAF |
T9206 |
10091-10097 |
NN |
denotes |
signal |
T9207 |
10085-10086 |
HYPH |
denotes |
- |
T9208 |
10086-10087 |
CD |
denotes |
1 |
T9209 |
10088-10090 |
NN |
denotes |
IF |
T9210 |
10097-10098 |
-RRB- |
denotes |
) |
T9211 |
10106-10111 |
RB |
denotes |
still |
T9212 |
10112-10118 |
JJ |
denotes |
active |
T9213 |
10119-10122 |
IN |
denotes |
for |
T9214 |
10123-10134 |
NN |
denotes |
replication |
T9215 |
10134-10136 |
, |
denotes |
, |
T9216 |
10136-10138 |
IN |
denotes |
as |
T9217 |
10139-10147 |
VBN |
denotes |
revealed |
T9218 |
10148-10150 |
IN |
denotes |
by |
T9219 |
10151-10164 |
NN |
denotes |
incorporation |
T9220 |
10165-10167 |
IN |
denotes |
of |
T9221 |
10168-10171 |
DT |
denotes |
the |
T9222 |
10182-10188 |
NN |
denotes |
analog |
T9223 |
10172-10181 |
NN |
denotes |
thymidine |
T9224 |
10189-10206 |
NN |
denotes |
bromodeoxyuridine |
T9225 |
10207-10208 |
-LRB- |
denotes |
( |
T9226 |
10208-10212 |
NN |
denotes |
BrdU |
T9227 |
10212-10213 |
-RRB- |
denotes |
) |
T9228 |
10214-10215 |
-LRB- |
denotes |
( |
T9229 |
10222-10224 |
NN |
denotes |
3A |
T9230 |
10215-10221 |
NN |
denotes |
Figure |
T9231 |
10224-10225 |
-RRB- |
denotes |
) |
T9232 |
10225-10227 |
, |
denotes |
, |
T9233 |
10227-10230 |
DT |
denotes |
the |
T9234 |
10273-10280 |
NN |
denotes |
pattern |
T9235 |
10231-10235 |
NN |
denotes |
PCNA |
T9236 |
10236-10237 |
-LRB- |
denotes |
( |
T9237 |
10237-10250 |
VBG |
denotes |
proliferating |
T9238 |
10264-10271 |
NN |
denotes |
antigen |
T9239 |
10251-10255 |
NN |
denotes |
cell |
T9240 |
10256-10263 |
JJ |
denotes |
nuclear |
T9241 |
10271-10272 |
-RRB- |
denotes |
) |
T9242 |
10281-10282 |
-LRB- |
denotes |
( |
T9243 |
10289-10291 |
NN |
denotes |
3B |
T9244 |
10282-10288 |
NN |
denotes |
Figure |
T9245 |
10291-10292 |
-RRB- |
denotes |
) |
T9246 |
10292-10294 |
, |
denotes |
, |
T9247 |
10294-10297 |
CC |
denotes |
and |
T9248 |
10298-10302 |
NN |
denotes |
flow |
T9249 |
10313-10321 |
NN |
denotes |
analysis |
T9250 |
10303-10312 |
NN |
denotes |
cytometry |
T9251 |
10322-10323 |
-LRB- |
denotes |
( |
T9252 |
10330-10332 |
NN |
denotes |
3C |
T9253 |
10323-10329 |
NN |
denotes |
Figure |
T9254 |
10332-10333 |
-RRB- |
denotes |
) |
T9255 |
10333-10334 |
. |
denotes |
. |
T9256 |
10334-10443 |
sentence |
denotes |
Hence, in these cells, DNA replication persists despite the drastic changes in heterochromatin organization. |
T9257 |
10335-10340 |
RB |
denotes |
Hence |
T9258 |
10374-10382 |
VBZ |
denotes |
persists |
T9259 |
10340-10342 |
, |
denotes |
, |
T9260 |
10342-10344 |
IN |
denotes |
in |
T9261 |
10345-10350 |
DT |
denotes |
these |
T9262 |
10351-10356 |
NNS |
denotes |
cells |
T9263 |
10356-10358 |
, |
denotes |
, |
T9264 |
10358-10361 |
NN |
denotes |
DNA |
T9265 |
10362-10373 |
NN |
denotes |
replication |
T9266 |
10383-10390 |
IN |
denotes |
despite |
T9267 |
10391-10394 |
DT |
denotes |
the |
T9268 |
10403-10410 |
NNS |
denotes |
changes |
T9269 |
10395-10402 |
JJ |
denotes |
drastic |
T9270 |
10411-10413 |
IN |
denotes |
in |
T9271 |
10414-10429 |
NN |
denotes |
heterochromatin |
T9272 |
10430-10442 |
NN |
denotes |
organization |
T9273 |
10442-10443 |
. |
denotes |
. |
T9274 |
10443-10590 |
sentence |
denotes |
However, 24 h later, ES cells cease to proliferate and die, revealing that p150CAF-1 is required for an essential cellular process, as in embryos. |
T9275 |
10444-10451 |
RB |
denotes |
However |
T9276 |
10474-10479 |
VBP |
denotes |
cease |
T9277 |
10451-10453 |
, |
denotes |
, |
T9278 |
10453-10455 |
CD |
denotes |
24 |
T9279 |
10456-10457 |
NN |
denotes |
h |
T9280 |
10458-10463 |
RB |
denotes |
later |
T9281 |
10463-10465 |
, |
denotes |
, |
T9282 |
10465-10467 |
NN |
denotes |
ES |
T9283 |
10468-10473 |
NNS |
denotes |
cells |
T9284 |
10480-10482 |
TO |
denotes |
to |
T9285 |
10483-10494 |
VB |
denotes |
proliferate |
T9286 |
10495-10498 |
CC |
denotes |
and |
T9287 |
10499-10502 |
VB |
denotes |
die |
T9288 |
10502-10504 |
, |
denotes |
, |
T9289 |
10504-10513 |
VBG |
denotes |
revealing |
T9290 |
10514-10518 |
IN |
denotes |
that |
T9291 |
10532-10540 |
VBN |
denotes |
required |
T9292 |
10519-10526 |
NN |
denotes |
p150CAF |
T9293 |
10526-10527 |
HYPH |
denotes |
- |
T9294 |
10527-10528 |
CD |
denotes |
1 |
T9295 |
10529-10531 |
VBZ |
denotes |
is |
T9296 |
10541-10544 |
IN |
denotes |
for |
T9297 |
10545-10547 |
DT |
denotes |
an |
T9298 |
10567-10574 |
NN |
denotes |
process |
T9299 |
10548-10557 |
JJ |
denotes |
essential |
T9300 |
10558-10566 |
JJ |
denotes |
cellular |
T9301 |
10574-10576 |
, |
denotes |
, |
T9302 |
10576-10578 |
IN |
denotes |
as |
T9303 |
10579-10581 |
IN |
denotes |
in |
T9304 |
10582-10589 |
NNS |
denotes |
embryos |
T9305 |
10589-10590 |
. |
denotes |
. |
T9306 |
10590-10802 |
sentence |
denotes |
Altogether, our data demonstrate that loss of p150CAF-1 function in ES cells and early embryos alters first the organization of heterochromatin in the nucleus and, in a subsequent step, cell cycle and viability. |
T9307 |
10591-10601 |
RB |
denotes |
Altogether |
T9308 |
10612-10623 |
VBP |
denotes |
demonstrate |
T9309 |
10601-10603 |
, |
denotes |
, |
T9310 |
10603-10606 |
PRP$ |
denotes |
our |
T9311 |
10607-10611 |
NNS |
denotes |
data |
T9312 |
10624-10628 |
IN |
denotes |
that |
T9313 |
10686-10692 |
VBZ |
denotes |
alters |
T9314 |
10629-10633 |
NN |
denotes |
loss |
T9315 |
10634-10636 |
IN |
denotes |
of |
T9316 |
10637-10644 |
NN |
denotes |
p150CAF |
T9317 |
10647-10655 |
NN |
denotes |
function |
T9318 |
10644-10645 |
HYPH |
denotes |
- |
T9319 |
10645-10646 |
CD |
denotes |
1 |
T9320 |
10656-10658 |
IN |
denotes |
in |
T9321 |
10659-10661 |
NN |
denotes |
ES |
T9322 |
10662-10667 |
NNS |
denotes |
cells |
T9323 |
10668-10671 |
CC |
denotes |
and |
T9324 |
10672-10677 |
JJ |
denotes |
early |
T9325 |
10678-10685 |
NNS |
denotes |
embryos |
T9326 |
10693-10698 |
RB |
denotes |
first |
T9327 |
10699-10702 |
DT |
denotes |
the |
T9328 |
10703-10715 |
NN |
denotes |
organization |
T9329 |
10716-10718 |
IN |
denotes |
of |
T9330 |
10719-10734 |
NN |
denotes |
heterochromatin |
T9331 |
10735-10737 |
IN |
denotes |
in |
T9332 |
10738-10741 |
DT |
denotes |
the |
T9333 |
10742-10749 |
NN |
denotes |
nucleus |
T9334 |
10750-10753 |
CC |
denotes |
and |
T9335 |
10753-10755 |
, |
denotes |
, |
T9336 |
10755-10757 |
IN |
denotes |
in |
T9337 |
10782-10787 |
NN |
denotes |
cycle |
T9338 |
10758-10759 |
DT |
denotes |
a |
T9339 |
10771-10775 |
NN |
denotes |
step |
T9340 |
10760-10770 |
JJ |
denotes |
subsequent |
T9341 |
10775-10777 |
, |
denotes |
, |
T9342 |
10777-10781 |
NN |
denotes |
cell |
T9343 |
10788-10791 |
CC |
denotes |
and |
T9344 |
10792-10801 |
NN |
denotes |
viability |
T9345 |
10801-10802 |
. |
denotes |
. |
T9346 |
10802-12372 |
sentence |
denotes |
Figure 3 p150CAF-1 Depletion and Loss of Heterochromatin Organization Are Compatible with Active DNA Replication in ES Cells
(A) ES cells were transfected with p150CAF-1 or control siRNA vectors. After 3 d under puromycin selection, cells were pulse-labeled for 10 min with BrdU and immediately analyzed by IF. No significant difference in BrdU incorporation could be detected between control and p150CAF-1-depleted cells. The right-hand image shows the merge between the p150CAF-1 and BrdU fluorescence. Scale bar = 10 μm.
(B) Immunodetection of PCNA (red) revealed no significant difference in the formation of replication foci between control and p150CAF-1-depleted ES cells. Immunodetection of p150CAF-1 is shown in green and the merging of p150CAF-1 and PCNA appears in yellow.
(C) Flow cytometry analysis showed a similar cell-cycle profile for control and p150CAF-1-depleted ES cells. Results are presented as the percentage of cells in each phase of the cell cycle (G1, S, G2/M), as defined by BrdU incorporation and DNA content. Data presented are the mean of three independent experiments; error bars indicate the standard deviation. All experiments were performed 3 d after transfection of the siRNA vectors. In order to better characterize the defects in heterochromatin organization, we performed two-color DNA fluorescence in situ hybridization (FISH) experiments to reveal the spatial distribution of pericentric and centric chromosomal regions, which in mouse are mainly composed of large blocks of major and minor satellite repeats, respectively [20]. |
T9347 |
12024-12026 |
IN |
denotes |
In |
T9348 |
12104-12113 |
VBD |
denotes |
performed |
T9349 |
12027-12032 |
NN |
denotes |
order |
T9350 |
12033-12035 |
TO |
denotes |
to |
T9351 |
12043-12055 |
VB |
denotes |
characterize |
T9352 |
12036-12042 |
RBR |
denotes |
better |
T9353 |
12056-12059 |
DT |
denotes |
the |
T9354 |
12060-12067 |
NNS |
denotes |
defects |
T9355 |
12068-12070 |
IN |
denotes |
in |
T9356 |
12071-12086 |
NN |
denotes |
heterochromatin |
T9357 |
12087-12099 |
NN |
denotes |
organization |
T9358 |
12099-12101 |
, |
denotes |
, |
T9359 |
12101-12103 |
PRP |
denotes |
we |
T9360 |
12114-12117 |
CD |
denotes |
two |
T9361 |
12118-12123 |
NN |
denotes |
color |
T9362 |
12117-12118 |
HYPH |
denotes |
- |
T9363 |
12170-12181 |
NNS |
denotes |
experiments |
T9364 |
12124-12127 |
NN |
denotes |
DNA |
T9365 |
12128-12140 |
NN |
denotes |
fluorescence |
T9366 |
12149-12162 |
NN |
denotes |
hybridization |
T9367 |
12141-12143 |
FW |
denotes |
in |
T9368 |
12144-12148 |
FW |
denotes |
situ |
T9369 |
12163-12164 |
-LRB- |
denotes |
( |
T9370 |
12164-12168 |
NN |
denotes |
FISH |
T9371 |
12168-12169 |
-RRB- |
denotes |
) |
T9372 |
12182-12184 |
TO |
denotes |
to |
T9373 |
12185-12191 |
VB |
denotes |
reveal |
T9374 |
12192-12195 |
DT |
denotes |
the |
T9375 |
12204-12216 |
NN |
denotes |
distribution |
T9376 |
12196-12203 |
JJ |
denotes |
spatial |
T9377 |
12217-12219 |
IN |
denotes |
of |
T9378 |
12220-12231 |
JJ |
denotes |
pericentric |
T9379 |
12256-12263 |
NNS |
denotes |
regions |
T9380 |
12232-12235 |
CC |
denotes |
and |
T9381 |
12236-12243 |
JJ |
denotes |
centric |
T9382 |
12244-12255 |
JJ |
denotes |
chromosomal |
T9383 |
12263-12265 |
, |
denotes |
, |
T9384 |
12265-12270 |
WDT |
denotes |
which |
T9385 |
12291-12299 |
VBN |
denotes |
composed |
T9386 |
12271-12273 |
IN |
denotes |
in |
T9387 |
12274-12279 |
NN |
denotes |
mouse |
T9388 |
12280-12283 |
VBP |
denotes |
are |
T9389 |
12284-12290 |
RB |
denotes |
mainly |
T9390 |
12300-12302 |
IN |
denotes |
of |
T9391 |
12303-12308 |
JJ |
denotes |
large |
T9392 |
12309-12315 |
NNS |
denotes |
blocks |
T9393 |
12316-12318 |
IN |
denotes |
of |
T9394 |
12319-12324 |
JJ |
denotes |
major |
T9395 |
12345-12352 |
NNS |
denotes |
repeats |
T9396 |
12325-12328 |
CC |
denotes |
and |
T9397 |
12329-12334 |
JJ |
denotes |
minor |
T9398 |
12335-12344 |
NN |
denotes |
satellite |
T9399 |
12352-12354 |
, |
denotes |
, |
T9400 |
12354-12366 |
RB |
denotes |
respectively |
T9401 |
12367-12368 |
-LRB- |
denotes |
[ |
T9402 |
12368-12370 |
CD |
denotes |
20 |
T9403 |
12370-12371 |
-RRB- |
denotes |
] |
T9404 |
12371-12372 |
. |
denotes |
. |
T9405 |
12372-12550 |
sentence |
denotes |
Pericentric domains from different chromosomes form clusters, which are revealed by FISH as large spots that coincide with DAPI-dense foci in the interphase nucleus (Figure 4A). |
T9406 |
12373-12384 |
JJ |
denotes |
Pericentric |
T9407 |
12385-12392 |
NNS |
denotes |
domains |
T9408 |
12420-12424 |
VBP |
denotes |
form |
T9409 |
12393-12397 |
IN |
denotes |
from |
T9410 |
12398-12407 |
JJ |
denotes |
different |
T9411 |
12408-12419 |
NNS |
denotes |
chromosomes |
T9412 |
12425-12433 |
NNS |
denotes |
clusters |
T9413 |
12433-12435 |
, |
denotes |
, |
T9414 |
12435-12440 |
WDT |
denotes |
which |
T9415 |
12445-12453 |
VBN |
denotes |
revealed |
T9416 |
12441-12444 |
VBP |
denotes |
are |
T9417 |
12454-12456 |
IN |
denotes |
by |
T9418 |
12457-12461 |
NN |
denotes |
FISH |
T9419 |
12462-12464 |
IN |
denotes |
as |
T9420 |
12465-12470 |
JJ |
denotes |
large |
T9421 |
12471-12476 |
NNS |
denotes |
spots |
T9422 |
12477-12481 |
WDT |
denotes |
that |
T9423 |
12482-12490 |
VBP |
denotes |
coincide |
T9424 |
12491-12495 |
IN |
denotes |
with |
T9425 |
12496-12500 |
NN |
denotes |
DAPI |
T9426 |
12501-12506 |
JJ |
denotes |
dense |
T9427 |
12500-12501 |
HYPH |
denotes |
- |
T9428 |
12507-12511 |
NNS |
denotes |
foci |
T9429 |
12512-12514 |
IN |
denotes |
in |
T9430 |
12515-12518 |
DT |
denotes |
the |
T9431 |
12530-12537 |
NN |
denotes |
nucleus |
T9432 |
12519-12529 |
NN |
denotes |
interphase |
T9433 |
12538-12539 |
-LRB- |
denotes |
( |
T9434 |
12546-12548 |
NN |
denotes |
4A |
T9435 |
12539-12545 |
NN |
denotes |
Figure |
T9436 |
12548-12549 |
-RRB- |
denotes |
) |
T9437 |
12549-12550 |
. |
denotes |
. |
T9438 |
12550-12649 |
sentence |
denotes |
At the periphery of each pericentric domain, centric regions form individual entities (Figure 4A). |
T9439 |
12551-12553 |
IN |
denotes |
At |
T9440 |
12612-12616 |
VBP |
denotes |
form |
T9441 |
12554-12557 |
DT |
denotes |
the |
T9442 |
12558-12567 |
NN |
denotes |
periphery |
T9443 |
12568-12570 |
IN |
denotes |
of |
T9444 |
12571-12575 |
DT |
denotes |
each |
T9445 |
12588-12594 |
NN |
denotes |
domain |
T9446 |
12576-12587 |
JJ |
denotes |
pericentric |
T9447 |
12594-12596 |
, |
denotes |
, |
T9448 |
12596-12603 |
JJ |
denotes |
centric |
T9449 |
12604-12611 |
NNS |
denotes |
regions |
T9450 |
12617-12627 |
JJ |
denotes |
individual |
T9451 |
12628-12636 |
NNS |
denotes |
entities |
T9452 |
12637-12638 |
-LRB- |
denotes |
( |
T9453 |
12645-12647 |
NN |
denotes |
4A |
T9454 |
12638-12644 |
NN |
denotes |
Figure |
T9455 |
12647-12648 |
-RRB- |
denotes |
) |
T9456 |
12648-12649 |
. |
denotes |
. |
T9457 |
12649-12818 |
sentence |
denotes |
In p150CAF-1 RNAi-depleted cells, we observed a disruption of pericentric heterochromatin clusters that coincides with the disappearance of DAPI-dense foci (Figure 4B). |
T9458 |
12650-12652 |
IN |
denotes |
In |
T9459 |
12687-12695 |
VBD |
denotes |
observed |
T9460 |
12653-12660 |
NN |
denotes |
p150CAF |
T9461 |
12677-12682 |
NNS |
denotes |
cells |
T9462 |
12660-12661 |
HYPH |
denotes |
- |
T9463 |
12661-12662 |
CD |
denotes |
1 |
T9464 |
12663-12667 |
NN |
denotes |
RNAi |
T9465 |
12668-12676 |
VBN |
denotes |
depleted |
T9466 |
12667-12668 |
HYPH |
denotes |
- |
T9467 |
12682-12684 |
, |
denotes |
, |
T9468 |
12684-12686 |
PRP |
denotes |
we |
T9469 |
12696-12697 |
DT |
denotes |
a |
T9470 |
12698-12708 |
NN |
denotes |
disruption |
T9471 |
12709-12711 |
IN |
denotes |
of |
T9472 |
12712-12723 |
JJ |
denotes |
pericentric |
T9473 |
12740-12748 |
NNS |
denotes |
clusters |
T9474 |
12724-12739 |
NN |
denotes |
heterochromatin |
T9475 |
12749-12753 |
WDT |
denotes |
that |
T9476 |
12754-12763 |
VBZ |
denotes |
coincides |
T9477 |
12764-12768 |
IN |
denotes |
with |
T9478 |
12769-12772 |
DT |
denotes |
the |
T9479 |
12773-12786 |
NN |
denotes |
disappearance |
T9480 |
12787-12789 |
IN |
denotes |
of |
T9481 |
12790-12794 |
NN |
denotes |
DAPI |
T9482 |
12795-12800 |
JJ |
denotes |
dense |
T9483 |
12794-12795 |
HYPH |
denotes |
- |
T9484 |
12801-12805 |
NNS |
denotes |
foci |
T9485 |
12806-12807 |
-LRB- |
denotes |
( |
T9486 |
12814-12816 |
NN |
denotes |
4B |
T9487 |
12807-12813 |
NN |
denotes |
Figure |
T9488 |
12816-12817 |
-RRB- |
denotes |
) |
T9489 |
12817-12818 |
. |
denotes |
. |
T9490 |
12818-13006 |
sentence |
denotes |
Individual pericentric domains from single chromosomes are now found either isolated or aggregated in a pattern less dense than the regular clusters observed in control cells (Figure 4B). |
T9491 |
12819-12829 |
JJ |
denotes |
Individual |
T9492 |
12842-12849 |
NNS |
denotes |
domains |
T9493 |
12830-12841 |
JJ |
denotes |
pericentric |
T9494 |
12882-12887 |
VBN |
denotes |
found |
T9495 |
12850-12854 |
IN |
denotes |
from |
T9496 |
12855-12861 |
JJ |
denotes |
single |
T9497 |
12862-12873 |
NNS |
denotes |
chromosomes |
T9498 |
12874-12877 |
VBP |
denotes |
are |
T9499 |
12878-12881 |
RB |
denotes |
now |
T9500 |
12888-12894 |
CC |
denotes |
either |
T9501 |
12895-12903 |
JJ |
denotes |
isolated |
T9502 |
12904-12906 |
CC |
denotes |
or |
T9503 |
12907-12917 |
JJ |
denotes |
aggregated |
T9504 |
12918-12920 |
IN |
denotes |
in |
T9505 |
12921-12922 |
DT |
denotes |
a |
T9506 |
12923-12930 |
NN |
denotes |
pattern |
T9507 |
12931-12935 |
RBR |
denotes |
less |
T9508 |
12936-12941 |
JJ |
denotes |
dense |
T9509 |
12942-12946 |
IN |
denotes |
than |
T9510 |
12947-12950 |
DT |
denotes |
the |
T9511 |
12959-12967 |
NNS |
denotes |
clusters |
T9512 |
12951-12958 |
JJ |
denotes |
regular |
T9513 |
12968-12976 |
VBN |
denotes |
observed |
T9514 |
12977-12979 |
IN |
denotes |
in |
T9515 |
12980-12987 |
NN |
denotes |
control |
T9516 |
12988-12993 |
NNS |
denotes |
cells |
T9517 |
12994-12995 |
-LRB- |
denotes |
( |
T9518 |
13002-13004 |
NN |
denotes |
4B |
T9519 |
12995-13001 |
NN |
denotes |
Figure |
T9520 |
13004-13005 |
-RRB- |
denotes |
) |
T9521 |
13005-13006 |
. |
denotes |
. |
T9522 |
13006-13160 |
sentence |
denotes |
Such aggregates, which are often found at the nuclear periphery, are also revealed by DAPI staining as a typical signature of p150CAF-1 loss-of-function. |
T9523 |
13007-13011 |
JJ |
denotes |
Such |
T9524 |
13012-13022 |
NNS |
denotes |
aggregates |
T9525 |
13081-13089 |
VBN |
denotes |
revealed |
T9526 |
13022-13024 |
, |
denotes |
, |
T9527 |
13024-13029 |
WDT |
denotes |
which |
T9528 |
13040-13045 |
VBN |
denotes |
found |
T9529 |
13030-13033 |
VBP |
denotes |
are |
T9530 |
13034-13039 |
RB |
denotes |
often |
T9531 |
13046-13048 |
IN |
denotes |
at |
T9532 |
13049-13052 |
DT |
denotes |
the |
T9533 |
13061-13070 |
NN |
denotes |
periphery |
T9534 |
13053-13060 |
JJ |
denotes |
nuclear |
T9535 |
13070-13072 |
, |
denotes |
, |
T9536 |
13072-13075 |
VBP |
denotes |
are |
T9537 |
13076-13080 |
RB |
denotes |
also |
T9538 |
13090-13092 |
IN |
denotes |
by |
T9539 |
13093-13097 |
NN |
denotes |
DAPI |
T9540 |
13098-13106 |
NN |
denotes |
staining |
T9541 |
13107-13109 |
IN |
denotes |
as |
T9542 |
13110-13111 |
DT |
denotes |
a |
T9543 |
13120-13129 |
NN |
denotes |
signature |
T9544 |
13112-13119 |
JJ |
denotes |
typical |
T9545 |
13130-13132 |
IN |
denotes |
of |
T9546 |
13133-13140 |
NN |
denotes |
p150CAF |
T9547 |
13140-13141 |
HYPH |
denotes |
- |
T9548 |
13141-13142 |
CD |
denotes |
1 |
T9549 |
13143-13147 |
NN |
denotes |
loss |
T9550 |
13147-13148 |
HYPH |
denotes |
- |
T9551 |
13148-13150 |
IN |
denotes |
of |
T9552 |
13150-13151 |
HYPH |
denotes |
- |
T9553 |
13151-13159 |
NN |
denotes |
function |
T9554 |
13159-13160 |
. |
denotes |
. |
T9555 |
13160-13467 |
sentence |
denotes |
Quantification of fluorescence along a line randomly drawn across the nucleus revealed lower fluorescence intensity and a broader distribution of DAPI and major satellite hybridization signals in p150CAF-1-depleted cells (Figure 4C and 4D), indicating decondensation of pericentric heterochromatin domains. |
T9556 |
13161-13175 |
NN |
denotes |
Quantification |
T9557 |
13239-13247 |
VBD |
denotes |
revealed |
T9558 |
13176-13178 |
IN |
denotes |
of |
T9559 |
13179-13191 |
NN |
denotes |
fluorescence |
T9560 |
13192-13197 |
IN |
denotes |
along |
T9561 |
13198-13199 |
DT |
denotes |
a |
T9562 |
13200-13204 |
NN |
denotes |
line |
T9563 |
13205-13213 |
RB |
denotes |
randomly |
T9564 |
13214-13219 |
VBN |
denotes |
drawn |
T9565 |
13220-13226 |
IN |
denotes |
across |
T9566 |
13227-13230 |
DT |
denotes |
the |
T9567 |
13231-13238 |
NN |
denotes |
nucleus |
T9568 |
13248-13253 |
JJR |
denotes |
lower |
T9569 |
13267-13276 |
NN |
denotes |
intensity |
T9570 |
13254-13266 |
NN |
denotes |
fluorescence |
T9571 |
13277-13280 |
CC |
denotes |
and |
T9572 |
13281-13282 |
DT |
denotes |
a |
T9573 |
13291-13303 |
NN |
denotes |
distribution |
T9574 |
13283-13290 |
JJR |
denotes |
broader |
T9575 |
13304-13306 |
IN |
denotes |
of |
T9576 |
13307-13311 |
NN |
denotes |
DAPI |
T9577 |
13312-13315 |
CC |
denotes |
and |
T9578 |
13316-13321 |
JJ |
denotes |
major |
T9579 |
13346-13353 |
NNS |
denotes |
signals |
T9580 |
13322-13331 |
NN |
denotes |
satellite |
T9581 |
13332-13345 |
NN |
denotes |
hybridization |
T9582 |
13354-13356 |
IN |
denotes |
in |
T9583 |
13357-13364 |
NN |
denotes |
p150CAF |
T9584 |
13367-13375 |
VBN |
denotes |
depleted |
T9585 |
13364-13365 |
HYPH |
denotes |
- |
T9586 |
13365-13366 |
CD |
denotes |
1 |
T9587 |
13366-13367 |
HYPH |
denotes |
- |
T9588 |
13376-13381 |
NNS |
denotes |
cells |
T9589 |
13382-13383 |
-LRB- |
denotes |
( |
T9590 |
13390-13392 |
NN |
denotes |
4C |
T9591 |
13383-13389 |
NN |
denotes |
Figure |
T9592 |
13393-13396 |
CC |
denotes |
and |
T9593 |
13397-13399 |
NN |
denotes |
4D |
T9594 |
13399-13400 |
-RRB- |
denotes |
) |
T9595 |
13400-13402 |
, |
denotes |
, |
T9596 |
13402-13412 |
VBG |
denotes |
indicating |
T9597 |
13413-13427 |
NN |
denotes |
decondensation |
T9598 |
13428-13430 |
IN |
denotes |
of |
T9599 |
13431-13442 |
JJ |
denotes |
pericentric |
T9600 |
13459-13466 |
NNS |
denotes |
domains |
T9601 |
13443-13458 |
NN |
denotes |
heterochromatin |
T9602 |
13466-13467 |
. |
denotes |
. |
T9603 |
13467-13634 |
sentence |
denotes |
In contrast, centric domains showed an intensity and shape similar to control cells, suggesting that they remain unaffected by p150CAF-1 depletion (Figure 4C and 4D). |
T9604 |
13468-13470 |
IN |
denotes |
In |
T9605 |
13497-13503 |
VBD |
denotes |
showed |
T9606 |
13471-13479 |
NN |
denotes |
contrast |
T9607 |
13479-13481 |
, |
denotes |
, |
T9608 |
13481-13488 |
JJ |
denotes |
centric |
T9609 |
13489-13496 |
NNS |
denotes |
domains |
T9610 |
13504-13506 |
DT |
denotes |
an |
T9611 |
13507-13516 |
NN |
denotes |
intensity |
T9612 |
13517-13520 |
CC |
denotes |
and |
T9613 |
13521-13526 |
NN |
denotes |
shape |
T9614 |
13527-13534 |
JJ |
denotes |
similar |
T9615 |
13535-13537 |
IN |
denotes |
to |
T9616 |
13538-13545 |
NN |
denotes |
control |
T9617 |
13546-13551 |
NNS |
denotes |
cells |
T9618 |
13551-13553 |
, |
denotes |
, |
T9619 |
13553-13563 |
VBG |
denotes |
suggesting |
T9620 |
13564-13568 |
IN |
denotes |
that |
T9621 |
13574-13580 |
VBP |
denotes |
remain |
T9622 |
13569-13573 |
PRP |
denotes |
they |
T9623 |
13581-13591 |
JJ |
denotes |
unaffected |
T9624 |
13592-13594 |
IN |
denotes |
by |
T9625 |
13595-13602 |
NN |
denotes |
p150CAF |
T9626 |
13605-13614 |
NN |
denotes |
depletion |
T9627 |
13602-13603 |
HYPH |
denotes |
- |
T9628 |
13603-13604 |
CD |
denotes |
1 |
T9629 |
13615-13616 |
-LRB- |
denotes |
( |
T9630 |
13623-13625 |
NN |
denotes |
4C |
T9631 |
13616-13622 |
NN |
denotes |
Figure |
T9632 |
13626-13629 |
CC |
denotes |
and |
T9633 |
13630-13632 |
NN |
denotes |
4D |
T9634 |
13632-13633 |
-RRB- |
denotes |
) |
T9635 |
13633-13634 |
. |
denotes |
. |
T9636 |
13634-13778 |
sentence |
denotes |
In conclusion, these results show that p150CAF-1 is required for the proper condensation and clustering of pericentric heterochromatin domains. |
T9637 |
13635-13637 |
IN |
denotes |
In |
T9638 |
13664-13668 |
VBP |
denotes |
show |
T9639 |
13638-13648 |
NN |
denotes |
conclusion |
T9640 |
13648-13650 |
, |
denotes |
, |
T9641 |
13650-13655 |
DT |
denotes |
these |
T9642 |
13656-13663 |
NNS |
denotes |
results |
T9643 |
13669-13673 |
IN |
denotes |
that |
T9644 |
13687-13695 |
VBN |
denotes |
required |
T9645 |
13674-13681 |
NN |
denotes |
p150CAF |
T9646 |
13681-13682 |
HYPH |
denotes |
- |
T9647 |
13682-13683 |
CD |
denotes |
1 |
T9648 |
13684-13686 |
VBZ |
denotes |
is |
T9649 |
13696-13699 |
IN |
denotes |
for |
T9650 |
13700-13703 |
DT |
denotes |
the |
T9651 |
13711-13723 |
NN |
denotes |
condensation |
T9652 |
13704-13710 |
JJ |
denotes |
proper |
T9653 |
13724-13727 |
CC |
denotes |
and |
T9654 |
13728-13738 |
NN |
denotes |
clustering |
T9655 |
13739-13741 |
IN |
denotes |
of |
T9656 |
13742-13753 |
JJ |
denotes |
pericentric |
T9657 |
13770-13777 |
NNS |
denotes |
domains |
T9658 |
13754-13769 |
NN |
denotes |
heterochromatin |
T9659 |
13777-13778 |
. |
denotes |
. |
T9660 |
13778-15733 |
sentence |
denotes |
Figure 4 Depletion of p150CAF-1 Leads to Loss of Clustering, Altered Localization, and Decondensation of Pericentric Heterochromatin Domains
Distribution of pericentric (red) and centric (green) domains was analyzed in the interphase nuclei of mouse ES cells by DNA FISH, using major satellite (pSAT) [47] and minor satellite (pMR150) [48] DNA probes, respectively. (A) In ES cells expressing control (cont) siRNA, pericentric regions from several chromosomes associate in clusters (red). These chromocenters form foci as revealed by DAPI staining (left-hand image), while centric regions (green) remain independent entities at the periphery of these domains. The right-hand image shows the merge between the pericentric and centric FISH signals.
(B) The organization of pericentric domains was altered in cells expressing p150CAF-1 siRNA. Instead of forming well-defined chromocenters, pericentric domains were found either isolated or associated in heterogeneous aggregates of various sizes, often at the nuclear periphery. Scale bar = 10 μm.
(C) Control ES cells. Fluorescence was quantified along a line randomly drawn across the nucleus in the merged image and data were plotted. One can distinguish clear peaks corresponding to chromocenters (red) and the condensed minor satellites (green).
(D) ES cells expressing p150CAF-1 siRNA. p150CAF-1 depletion led to a lower fluorescence intensity and a broader distribution of signals corresponding to DAPI (blue) and major satellite hybridization (red, plot) while the organization of the minor satellites remained unaffected. Insets in the right-hand images show a typical chromocenter in control cells (C) and a disrupted chromocenter in p150CAF-1-depleted cells (D). Given the known role of CAF-1 in the deposition of histone H3.1 and H4 associated with DNA synthesis [11,12], we wondered whether this defect in higher-order chromatin organization could reflect an aberrant nucleosomal organization. |
T9661 |
15501-15506 |
VBN |
denotes |
Given |
T9662 |
15614-15622 |
VBD |
denotes |
wondered |
T9663 |
15507-15510 |
DT |
denotes |
the |
T9664 |
15517-15521 |
NN |
denotes |
role |
T9665 |
15511-15516 |
VBN |
denotes |
known |
T9666 |
15522-15524 |
IN |
denotes |
of |
T9667 |
15525-15528 |
NN |
denotes |
CAF |
T9668 |
15528-15529 |
HYPH |
denotes |
- |
T9669 |
15529-15530 |
CD |
denotes |
1 |
T9670 |
15531-15533 |
IN |
denotes |
in |
T9671 |
15534-15537 |
DT |
denotes |
the |
T9672 |
15538-15548 |
NN |
denotes |
deposition |
T9673 |
15549-15551 |
IN |
denotes |
of |
T9674 |
15552-15559 |
NN |
denotes |
histone |
T9675 |
15560-15564 |
NN |
denotes |
H3.1 |
T9676 |
15565-15568 |
CC |
denotes |
and |
T9677 |
15569-15571 |
NN |
denotes |
H4 |
T9678 |
15572-15582 |
VBN |
denotes |
associated |
T9679 |
15583-15587 |
IN |
denotes |
with |
T9680 |
15588-15591 |
NN |
denotes |
DNA |
T9681 |
15592-15601 |
NN |
denotes |
synthesis |
T9682 |
15602-15603 |
-LRB- |
denotes |
[ |
T9683 |
15606-15608 |
CD |
denotes |
12 |
T9684 |
15603-15605 |
CD |
denotes |
11 |
T9685 |
15605-15606 |
, |
denotes |
, |
T9686 |
15608-15609 |
-RRB- |
denotes |
] |
T9687 |
15609-15611 |
, |
denotes |
, |
T9688 |
15611-15613 |
PRP |
denotes |
we |
T9689 |
15623-15630 |
IN |
denotes |
whether |
T9690 |
15688-15695 |
VB |
denotes |
reflect |
T9691 |
15631-15635 |
DT |
denotes |
this |
T9692 |
15636-15642 |
NN |
denotes |
defect |
T9693 |
15643-15645 |
IN |
denotes |
in |
T9694 |
15646-15652 |
JJR |
denotes |
higher |
T9695 |
15653-15658 |
NN |
denotes |
order |
T9696 |
15652-15653 |
HYPH |
denotes |
- |
T9697 |
15669-15681 |
NN |
denotes |
organization |
T9698 |
15659-15668 |
NN |
denotes |
chromatin |
T9699 |
15682-15687 |
MD |
denotes |
could |
T9700 |
15696-15698 |
DT |
denotes |
an |
T9701 |
15720-15732 |
NN |
denotes |
organization |
T9702 |
15699-15707 |
JJ |
denotes |
aberrant |
T9703 |
15708-15719 |
JJ |
denotes |
nucleosomal |
T9704 |
15732-15733 |
. |
denotes |
. |
T9705 |
15733-15960 |
sentence |
denotes |
Using DNase I and micrococcal nuclease (MNase) assays, we could not observe any significant difference between control and p150CAF-1-depleted ES cells at the level of bulk genome chromatin or at pericentric repeats (Figure 5). |
T9706 |
15734-15739 |
VBG |
denotes |
Using |
T9707 |
15802-15809 |
VB |
denotes |
observe |
T9708 |
15740-15745 |
NN |
denotes |
DNase |
T9709 |
15746-15747 |
CD |
denotes |
I |
T9710 |
15748-15751 |
CC |
denotes |
and |
T9711 |
15752-15763 |
JJ |
denotes |
micrococcal |
T9712 |
15764-15772 |
NN |
denotes |
nuclease |
T9713 |
15781-15787 |
NNS |
denotes |
assays |
T9714 |
15773-15774 |
-LRB- |
denotes |
( |
T9715 |
15774-15779 |
NN |
denotes |
MNase |
T9716 |
15779-15780 |
-RRB- |
denotes |
) |
T9717 |
15787-15789 |
, |
denotes |
, |
T9718 |
15789-15791 |
PRP |
denotes |
we |
T9719 |
15792-15797 |
MD |
denotes |
could |
T9720 |
15798-15801 |
RB |
denotes |
not |
T9721 |
15810-15813 |
DT |
denotes |
any |
T9722 |
15826-15836 |
NN |
denotes |
difference |
T9723 |
15814-15825 |
JJ |
denotes |
significant |
T9724 |
15837-15844 |
IN |
denotes |
between |
T9725 |
15845-15852 |
NN |
denotes |
control |
T9726 |
15879-15884 |
NNS |
denotes |
cells |
T9727 |
15853-15856 |
CC |
denotes |
and |
T9728 |
15857-15864 |
NN |
denotes |
p150CAF |
T9729 |
15867-15875 |
VBN |
denotes |
depleted |
T9730 |
15864-15865 |
HYPH |
denotes |
- |
T9731 |
15865-15866 |
CD |
denotes |
1 |
T9732 |
15866-15867 |
HYPH |
denotes |
- |
T9733 |
15876-15878 |
NN |
denotes |
ES |
T9734 |
15885-15887 |
IN |
denotes |
at |
T9735 |
15888-15891 |
DT |
denotes |
the |
T9736 |
15892-15897 |
NN |
denotes |
level |
T9737 |
15898-15900 |
IN |
denotes |
of |
T9738 |
15901-15905 |
NN |
denotes |
bulk |
T9739 |
15913-15922 |
NN |
denotes |
chromatin |
T9740 |
15906-15912 |
NN |
denotes |
genome |
T9741 |
15923-15925 |
CC |
denotes |
or |
T9742 |
15926-15928 |
IN |
denotes |
at |
T9743 |
15929-15940 |
JJ |
denotes |
pericentric |
T9744 |
15941-15948 |
NNS |
denotes |
repeats |
T9745 |
15949-15950 |
-LRB- |
denotes |
( |
T9746 |
15950-15956 |
NN |
denotes |
Figure |
T9747 |
15957-15958 |
CD |
denotes |
5 |
T9748 |
15958-15959 |
-RRB- |
denotes |
) |
T9749 |
15959-15960 |
. |
denotes |
. |
T9750 |
15960-16143 |
sentence |
denotes |
In a second series of experiments, we compared the association of histone H3 with chromatin in nuclei isolated from cells transfected with control and p150CAF-1 RNAi plasmid vectors. |
T9751 |
15961-15963 |
IN |
denotes |
In |
T9752 |
15999-16007 |
VBD |
denotes |
compared |
T9753 |
15964-15965 |
DT |
denotes |
a |
T9754 |
15973-15979 |
NN |
denotes |
series |
T9755 |
15966-15972 |
JJ |
denotes |
second |
T9756 |
15980-15982 |
IN |
denotes |
of |
T9757 |
15983-15994 |
NNS |
denotes |
experiments |
T9758 |
15994-15996 |
, |
denotes |
, |
T9759 |
15996-15998 |
PRP |
denotes |
we |
T9760 |
16008-16011 |
DT |
denotes |
the |
T9761 |
16012-16023 |
NN |
denotes |
association |
T9762 |
16024-16026 |
IN |
denotes |
of |
T9763 |
16027-16034 |
NN |
denotes |
histone |
T9764 |
16035-16037 |
NN |
denotes |
H3 |
T9765 |
16038-16042 |
IN |
denotes |
with |
T9766 |
16043-16052 |
NN |
denotes |
chromatin |
T9767 |
16053-16055 |
IN |
denotes |
in |
T9768 |
16056-16062 |
NNS |
denotes |
nuclei |
T9769 |
16063-16071 |
VBN |
denotes |
isolated |
T9770 |
16072-16076 |
IN |
denotes |
from |
T9771 |
16077-16082 |
NNS |
denotes |
cells |
T9772 |
16083-16094 |
VBN |
denotes |
transfected |
T9773 |
16095-16099 |
IN |
denotes |
with |
T9774 |
16100-16107 |
NN |
denotes |
control |
T9775 |
16135-16142 |
NNS |
denotes |
vectors |
T9776 |
16108-16111 |
CC |
denotes |
and |
T9777 |
16112-16119 |
NN |
denotes |
p150CAF |
T9778 |
16119-16120 |
HYPH |
denotes |
- |
T9779 |
16120-16121 |
CD |
denotes |
1 |
T9780 |
16122-16126 |
NN |
denotes |
RNAi |
T9781 |
16127-16134 |
NN |
denotes |
plasmid |
T9782 |
16142-16143 |
. |
denotes |
. |
T9783 |
16143-16249 |
sentence |
denotes |
Nuclei were incubated in buffers with different salt concentrations ranging from 100 mM NaCl to 1 M NaCl. |
T9784 |
16144-16150 |
NNS |
denotes |
Nuclei |
T9785 |
16156-16165 |
VBN |
denotes |
incubated |
T9786 |
16151-16155 |
VBD |
denotes |
were |
T9787 |
16166-16168 |
IN |
denotes |
in |
T9788 |
16169-16176 |
NNS |
denotes |
buffers |
T9789 |
16177-16181 |
IN |
denotes |
with |
T9790 |
16182-16191 |
JJ |
denotes |
different |
T9791 |
16197-16211 |
NNS |
denotes |
concentrations |
T9792 |
16192-16196 |
NN |
denotes |
salt |
T9793 |
16212-16219 |
VBG |
denotes |
ranging |
T9794 |
16220-16224 |
IN |
denotes |
from |
T9795 |
16225-16228 |
CD |
denotes |
100 |
T9796 |
16229-16231 |
NN |
denotes |
mM |
T9797 |
16232-16236 |
NN |
denotes |
NaCl |
T9798 |
16237-16239 |
IN |
denotes |
to |
T9799 |
16240-16241 |
CD |
denotes |
1 |
T9800 |
16242-16243 |
NN |
denotes |
M |
T9801 |
16244-16248 |
NN |
denotes |
NaCl |
T9802 |
16248-16249 |
. |
denotes |
. |
T9803 |
16249-16414 |
sentence |
denotes |
In all conditions, the amount of histone H3 remaining associated with chromatin was indistinguishable in control and p150CAF-1-depleted ES cells (unpublished data). |
T9804 |
16250-16252 |
IN |
denotes |
In |
T9805 |
16330-16333 |
VBD |
denotes |
was |
T9806 |
16253-16256 |
DT |
denotes |
all |
T9807 |
16257-16267 |
NNS |
denotes |
conditions |
T9808 |
16267-16269 |
, |
denotes |
, |
T9809 |
16269-16272 |
DT |
denotes |
the |
T9810 |
16273-16279 |
NN |
denotes |
amount |
T9811 |
16280-16282 |
IN |
denotes |
of |
T9812 |
16283-16290 |
NN |
denotes |
histone |
T9813 |
16291-16293 |
NN |
denotes |
H3 |
T9814 |
16294-16303 |
VBG |
denotes |
remaining |
T9815 |
16304-16314 |
VBN |
denotes |
associated |
T9816 |
16315-16319 |
IN |
denotes |
with |
T9817 |
16320-16329 |
NN |
denotes |
chromatin |
T9818 |
16334-16351 |
JJ |
denotes |
indistinguishable |
T9819 |
16352-16354 |
IN |
denotes |
in |
T9820 |
16355-16362 |
NN |
denotes |
control |
T9821 |
16389-16394 |
NNS |
denotes |
cells |
T9822 |
16363-16366 |
CC |
denotes |
and |
T9823 |
16367-16374 |
NN |
denotes |
p150CAF |
T9824 |
16377-16385 |
VBN |
denotes |
depleted |
T9825 |
16374-16375 |
HYPH |
denotes |
- |
T9826 |
16375-16376 |
CD |
denotes |
1 |
T9827 |
16376-16377 |
HYPH |
denotes |
- |
T9828 |
16386-16388 |
NN |
denotes |
ES |
T9829 |
16395-16396 |
-LRB- |
denotes |
( |
T9830 |
16408-16412 |
NNS |
denotes |
data |
T9831 |
16396-16407 |
JJ |
denotes |
unpublished |
T9832 |
16412-16413 |
-RRB- |
denotes |
) |
T9833 |
16413-16414 |
. |
denotes |
. |
T9834 |
16414-16580 |
sentence |
denotes |
Therefore, the loss of clustering and decondensation of pericentric heterochromatin are unlikely to be the consequence of severe defects in nucleosomal organization. |
T9835 |
16415-16424 |
RB |
denotes |
Therefore |
T9836 |
16499-16502 |
VBP |
denotes |
are |
T9837 |
16424-16426 |
, |
denotes |
, |
T9838 |
16426-16429 |
DT |
denotes |
the |
T9839 |
16430-16434 |
NN |
denotes |
loss |
T9840 |
16435-16437 |
IN |
denotes |
of |
T9841 |
16438-16448 |
NN |
denotes |
clustering |
T9842 |
16449-16452 |
CC |
denotes |
and |
T9843 |
16453-16467 |
NN |
denotes |
decondensation |
T9844 |
16468-16470 |
IN |
denotes |
of |
T9845 |
16471-16482 |
JJ |
denotes |
pericentric |
T9846 |
16483-16498 |
NN |
denotes |
heterochromatin |
T9847 |
16503-16511 |
JJ |
denotes |
unlikely |
T9848 |
16512-16514 |
TO |
denotes |
to |
T9849 |
16515-16517 |
VB |
denotes |
be |
T9850 |
16518-16521 |
DT |
denotes |
the |
T9851 |
16522-16533 |
NN |
denotes |
consequence |
T9852 |
16534-16536 |
IN |
denotes |
of |
T9853 |
16537-16543 |
JJ |
denotes |
severe |
T9854 |
16544-16551 |
NNS |
denotes |
defects |
T9855 |
16552-16554 |
IN |
denotes |
in |
T9856 |
16555-16566 |
JJ |
denotes |
nucleosomal |
T9857 |
16567-16579 |
NN |
denotes |
organization |
T9858 |
16579-16580 |
. |
denotes |
. |
T9859 |
16580-17252 |
sentence |
denotes |
Figure 5 Nucleosomal Organization Is Not Altered in p150CAF-1-Depleted ES Cells
Nuclei were prepared from ES cells transfected with control or p150CAF-1 siRNA vector. Nuclei were digested with increasing amounts of DNase I or MNase. (A) After digestion with the indicated nucleases, total DNA was prepared and run onto an agarose gel which was stained with ethidium bromide to reveal bulk genomic DNA.
(B) The DNA was blotted onto a nylon membrane, which was then hybridized with the α-32P-labeled pSAT major satellite repeat probe [47]. Our findings showed that the nuclear localization of HP1α is severely altered in Chaf1a −/− embryos and p150CAF-1-depleted ES cells. |
T9860 |
17120-17123 |
PRP$ |
denotes |
Our |
T9861 |
17124-17132 |
NNS |
denotes |
findings |
T9862 |
17133-17139 |
VBD |
denotes |
showed |
T9863 |
17140-17144 |
IN |
denotes |
that |
T9864 |
17190-17197 |
VBN |
denotes |
altered |
T9865 |
17145-17148 |
DT |
denotes |
the |
T9866 |
17157-17169 |
NN |
denotes |
localization |
T9867 |
17149-17156 |
JJ |
denotes |
nuclear |
T9868 |
17170-17172 |
IN |
denotes |
of |
T9869 |
17173-17177 |
NN |
denotes |
HP1α |
T9870 |
17178-17180 |
VBZ |
denotes |
is |
T9871 |
17181-17189 |
RB |
denotes |
severely |
T9872 |
17198-17200 |
IN |
denotes |
in |
T9873 |
17201-17207 |
NN |
denotes |
Chaf1a |
T9874 |
17212-17219 |
NNS |
denotes |
embryos |
T9875 |
17208-17209 |
SYM |
denotes |
− |
T9876 |
17209-17210 |
HYPH |
denotes |
/ |
T9877 |
17210-17211 |
SYM |
denotes |
− |
T9878 |
17220-17223 |
CC |
denotes |
and |
T9879 |
17224-17231 |
NN |
denotes |
p150CAF |
T9880 |
17234-17242 |
VBN |
denotes |
depleted |
T9881 |
17231-17232 |
HYPH |
denotes |
- |
T9882 |
17232-17233 |
CD |
denotes |
1 |
T9883 |
17233-17234 |
HYPH |
denotes |
- |
T9884 |
17246-17251 |
NNS |
denotes |
cells |
T9885 |
17243-17245 |
NN |
denotes |
ES |
T9886 |
17251-17252 |
. |
denotes |
. |
T9887 |
17252-17373 |
sentence |
denotes |
We therefore examined the status of other epigenetic marks previously shown to characterize pericentric heterochromatin. |
T9888 |
17253-17255 |
PRP |
denotes |
We |
T9889 |
17266-17274 |
VBD |
denotes |
examined |
T9890 |
17256-17265 |
RB |
denotes |
therefore |
T9891 |
17275-17278 |
DT |
denotes |
the |
T9892 |
17279-17285 |
NN |
denotes |
status |
T9893 |
17286-17288 |
IN |
denotes |
of |
T9894 |
17289-17294 |
JJ |
denotes |
other |
T9895 |
17306-17311 |
NNS |
denotes |
marks |
T9896 |
17295-17305 |
JJ |
denotes |
epigenetic |
T9897 |
17312-17322 |
RB |
denotes |
previously |
T9898 |
17323-17328 |
VBN |
denotes |
shown |
T9899 |
17329-17331 |
TO |
denotes |
to |
T9900 |
17332-17344 |
VB |
denotes |
characterize |
T9901 |
17345-17356 |
JJ |
denotes |
pericentric |
T9902 |
17357-17372 |
NN |
denotes |
heterochromatin |
T9903 |
17372-17373 |
. |
denotes |
. |
T9904 |
17373-17534 |
sentence |
denotes |
We first tested DNA CpG methylation [25] at major satellite repeats and found no significant difference between control and p150CAF-1-depleted cells (Figure 6). |
T9905 |
17374-17376 |
PRP |
denotes |
We |
T9906 |
17383-17389 |
VBD |
denotes |
tested |
T9907 |
17377-17382 |
RB |
denotes |
first |
T9908 |
17390-17393 |
NN |
denotes |
DNA |
T9909 |
17398-17409 |
NN |
denotes |
methylation |
T9910 |
17394-17397 |
NN |
denotes |
CpG |
T9911 |
17410-17411 |
-LRB- |
denotes |
[ |
T9912 |
17411-17413 |
CD |
denotes |
25 |
T9913 |
17413-17414 |
-RRB- |
denotes |
] |
T9914 |
17415-17417 |
IN |
denotes |
at |
T9915 |
17418-17423 |
JJ |
denotes |
major |
T9916 |
17434-17441 |
NNS |
denotes |
repeats |
T9917 |
17424-17433 |
NN |
denotes |
satellite |
T9918 |
17442-17445 |
CC |
denotes |
and |
T9919 |
17446-17451 |
VBD |
denotes |
found |
T9920 |
17452-17454 |
DT |
denotes |
no |
T9921 |
17467-17477 |
NN |
denotes |
difference |
T9922 |
17455-17466 |
JJ |
denotes |
significant |
T9923 |
17478-17485 |
IN |
denotes |
between |
T9924 |
17486-17493 |
NN |
denotes |
control |
T9925 |
17517-17522 |
NNS |
denotes |
cells |
T9926 |
17494-17497 |
CC |
denotes |
and |
T9927 |
17498-17505 |
NN |
denotes |
p150CAF |
T9928 |
17508-17516 |
VBN |
denotes |
depleted |
T9929 |
17505-17506 |
HYPH |
denotes |
- |
T9930 |
17506-17507 |
CD |
denotes |
1 |
T9931 |
17507-17508 |
HYPH |
denotes |
- |
T9932 |
17523-17524 |
-LRB- |
denotes |
( |
T9933 |
17524-17530 |
NN |
denotes |
Figure |
T9934 |
17531-17532 |
CD |
denotes |
6 |
T9935 |
17532-17533 |
-RRB- |
denotes |
) |
T9936 |
17533-17534 |
. |
denotes |
. |
T9937 |
17534-17681 |
sentence |
denotes |
As the LTM7 ES cell line that we used in our RNAi experiments is a female (XX) cell line, DNA methylation is globally reduced in these cells [26]. |
T9938 |
17535-17537 |
IN |
denotes |
As |
T9939 |
17597-17599 |
VBZ |
denotes |
is |
T9940 |
17538-17541 |
DT |
denotes |
the |
T9941 |
17555-17559 |
NN |
denotes |
line |
T9942 |
17542-17546 |
NN |
denotes |
LTM7 |
T9943 |
17547-17549 |
NN |
denotes |
ES |
T9944 |
17550-17554 |
NN |
denotes |
cell |
T9945 |
17560-17564 |
WDT |
denotes |
that |
T9946 |
17568-17572 |
VBD |
denotes |
used |
T9947 |
17565-17567 |
PRP |
denotes |
we |
T9948 |
17573-17575 |
IN |
denotes |
in |
T9949 |
17576-17579 |
PRP$ |
denotes |
our |
T9950 |
17585-17596 |
NNS |
denotes |
experiments |
T9951 |
17580-17584 |
NN |
denotes |
RNAi |
T9952 |
17653-17660 |
VBN |
denotes |
reduced |
T9953 |
17600-17601 |
DT |
denotes |
a |
T9954 |
17619-17623 |
NN |
denotes |
line |
T9955 |
17602-17608 |
JJ |
denotes |
female |
T9956 |
17609-17610 |
-LRB- |
denotes |
( |
T9957 |
17610-17612 |
NN |
denotes |
XX |
T9958 |
17612-17613 |
-RRB- |
denotes |
) |
T9959 |
17614-17618 |
NN |
denotes |
cell |
T9960 |
17623-17625 |
, |
denotes |
, |
T9961 |
17625-17628 |
NN |
denotes |
DNA |
T9962 |
17629-17640 |
NN |
denotes |
methylation |
T9963 |
17641-17643 |
VBZ |
denotes |
is |
T9964 |
17644-17652 |
RB |
denotes |
globally |
T9965 |
17661-17663 |
IN |
denotes |
in |
T9966 |
17664-17669 |
DT |
denotes |
these |
T9967 |
17670-17675 |
NNS |
denotes |
cells |
T9968 |
17676-17677 |
-LRB- |
denotes |
[ |
T9969 |
17677-17679 |
CD |
denotes |
26 |
T9970 |
17679-17680 |
-RRB- |
denotes |
] |
T9971 |
17680-17681 |
. |
denotes |
. |
T9972 |
17681-18087 |
sentence |
denotes |
To rule out the possibility that hypomethylation of pericentric repeats might contribute to the destabilization of heterochromatin domains in p150CAF-1-depleted ES cells, we tested our RNAi plasmid vector in a XY ES cell line, and confirmed that loss of p150CAF-1 leads to disruption of heterochromatin organization independently of the degree of DNA methylation at pericentric repeats (unpublished data). |
T9973 |
17682-17684 |
TO |
denotes |
To |
T9974 |
17685-17689 |
VB |
denotes |
rule |
T9975 |
17856-17862 |
VBD |
denotes |
tested |
T9976 |
17690-17693 |
RP |
denotes |
out |
T9977 |
17694-17697 |
DT |
denotes |
the |
T9978 |
17698-17709 |
NN |
denotes |
possibility |
T9979 |
17710-17714 |
IN |
denotes |
that |
T9980 |
17760-17770 |
VB |
denotes |
contribute |
T9981 |
17715-17730 |
NN |
denotes |
hypomethylation |
T9982 |
17731-17733 |
IN |
denotes |
of |
T9983 |
17734-17745 |
JJ |
denotes |
pericentric |
T9984 |
17746-17753 |
NNS |
denotes |
repeats |
T9985 |
17754-17759 |
MD |
denotes |
might |
T9986 |
17771-17773 |
IN |
denotes |
to |
T9987 |
17774-17777 |
DT |
denotes |
the |
T9988 |
17778-17793 |
NN |
denotes |
destabilization |
T9989 |
17794-17796 |
IN |
denotes |
of |
T9990 |
17797-17812 |
NN |
denotes |
heterochromatin |
T9991 |
17813-17820 |
NNS |
denotes |
domains |
T9992 |
17821-17823 |
IN |
denotes |
in |
T9993 |
17824-17831 |
NN |
denotes |
p150CAF |
T9994 |
17834-17842 |
VBN |
denotes |
depleted |
T9995 |
17831-17832 |
HYPH |
denotes |
- |
T9996 |
17832-17833 |
CD |
denotes |
1 |
T9997 |
17833-17834 |
HYPH |
denotes |
- |
T9998 |
17846-17851 |
NNS |
denotes |
cells |
T9999 |
17843-17845 |
NN |
denotes |
ES |
T10000 |
17851-17853 |
, |
denotes |
, |
T10001 |
17853-17855 |
PRP |
denotes |
we |
T10002 |
17863-17866 |
PRP$ |
denotes |
our |
T10003 |
17880-17886 |
NN |
denotes |
vector |
T10004 |
17867-17871 |
NN |
denotes |
RNAi |
T10005 |
17872-17879 |
NN |
denotes |
plasmid |
T10006 |
17887-17889 |
IN |
denotes |
in |
T10007 |
17890-17891 |
DT |
denotes |
a |
T10008 |
17903-17907 |
NN |
denotes |
line |
T10009 |
17892-17894 |
NN |
denotes |
XY |
T10010 |
17895-17897 |
NN |
denotes |
ES |
T10011 |
17898-17902 |
NN |
denotes |
cell |
T10012 |
17907-17909 |
, |
denotes |
, |
T10013 |
17909-17912 |
CC |
denotes |
and |
T10014 |
17913-17922 |
VBD |
denotes |
confirmed |
T10015 |
17923-17927 |
IN |
denotes |
that |
T10016 |
17946-17951 |
VBZ |
denotes |
leads |
T10017 |
17928-17932 |
NN |
denotes |
loss |
T10018 |
17933-17935 |
IN |
denotes |
of |
T10019 |
17936-17943 |
NN |
denotes |
p150CAF |
T10020 |
17943-17944 |
HYPH |
denotes |
- |
T10021 |
17944-17945 |
CD |
denotes |
1 |
T10022 |
17952-17954 |
IN |
denotes |
to |
T10023 |
17955-17965 |
NN |
denotes |
disruption |
T10024 |
17966-17968 |
IN |
denotes |
of |
T10025 |
17969-17984 |
NN |
denotes |
heterochromatin |
T10026 |
17985-17997 |
NN |
denotes |
organization |
T10027 |
17998-18011 |
RB |
denotes |
independently |
T10028 |
18012-18014 |
IN |
denotes |
of |
T10029 |
18015-18018 |
DT |
denotes |
the |
T10030 |
18019-18025 |
NN |
denotes |
degree |
T10031 |
18026-18028 |
IN |
denotes |
of |
T10032 |
18029-18032 |
NN |
denotes |
DNA |
T10033 |
18033-18044 |
NN |
denotes |
methylation |
T10034 |
18045-18047 |
IN |
denotes |
at |
T10035 |
18048-18059 |
JJ |
denotes |
pericentric |
T10036 |
18060-18067 |
NNS |
denotes |
repeats |
T10037 |
18068-18069 |
-LRB- |
denotes |
( |
T10038 |
18081-18085 |
NNS |
denotes |
data |
T10039 |
18069-18080 |
JJ |
denotes |
unpublished |
T10040 |
18085-18086 |
-RRB- |
denotes |
) |
T10041 |
18086-18087 |
. |
denotes |
. |
T10042 |
18087-18221 |
sentence |
denotes |
We next analyzed specific histone modifications [27] in chromatin immunoprecipitation (ChIP) experiments using native chromatin [28]. |
T10043 |
18088-18090 |
PRP |
denotes |
We |
T10044 |
18096-18104 |
VBD |
denotes |
analyzed |
T10045 |
18091-18095 |
RB |
denotes |
next |
T10046 |
18105-18113 |
JJ |
denotes |
specific |
T10047 |
18122-18135 |
NNS |
denotes |
modifications |
T10048 |
18114-18121 |
NN |
denotes |
histone |
T10049 |
18136-18137 |
-LRB- |
denotes |
[ |
T10050 |
18137-18139 |
CD |
denotes |
27 |
T10051 |
18139-18140 |
-RRB- |
denotes |
] |
T10052 |
18141-18143 |
IN |
denotes |
in |
T10053 |
18144-18153 |
NN |
denotes |
chromatin |
T10054 |
18154-18173 |
NN |
denotes |
immunoprecipitation |
T10055 |
18181-18192 |
NNS |
denotes |
experiments |
T10056 |
18174-18175 |
-LRB- |
denotes |
( |
T10057 |
18175-18179 |
NN |
denotes |
ChIP |
T10058 |
18179-18180 |
-RRB- |
denotes |
) |
T10059 |
18193-18198 |
VBG |
denotes |
using |
T10060 |
18199-18205 |
JJ |
denotes |
native |
T10061 |
18206-18215 |
NN |
denotes |
chromatin |
T10062 |
18216-18217 |
-LRB- |
denotes |
[ |
T10063 |
18217-18219 |
CD |
denotes |
28 |
T10064 |
18219-18220 |
-RRB- |
denotes |
] |
T10065 |
18220-18221 |
. |
denotes |
. |
T10066 |
18221-18388 |
sentence |
denotes |
We found that pericentric DNA was immunoprecipitated with a 2-fold lower efficiency with H4K20me3 [29,30] antibodies following p150CAF-1 depletion (Figure 7A and 7B). |
T10067 |
18222-18224 |
PRP |
denotes |
We |
T10068 |
18225-18230 |
VBD |
denotes |
found |
T10069 |
18231-18235 |
IN |
denotes |
that |
T10070 |
18256-18274 |
VBN |
denotes |
immunoprecipitated |
T10071 |
18236-18247 |
JJ |
denotes |
pericentric |
T10072 |
18248-18251 |
NN |
denotes |
DNA |
T10073 |
18252-18255 |
VBD |
denotes |
was |
T10074 |
18275-18279 |
IN |
denotes |
with |
T10075 |
18280-18281 |
DT |
denotes |
a |
T10076 |
18295-18305 |
NN |
denotes |
efficiency |
T10077 |
18282-18283 |
CD |
denotes |
2 |
T10078 |
18289-18294 |
JJR |
denotes |
lower |
T10079 |
18283-18284 |
HYPH |
denotes |
- |
T10080 |
18284-18288 |
RB |
denotes |
fold |
T10081 |
18306-18310 |
IN |
denotes |
with |
T10082 |
18311-18319 |
NN |
denotes |
H4K20me3 |
T10083 |
18328-18338 |
NNS |
denotes |
antibodies |
T10084 |
18320-18321 |
-LRB- |
denotes |
[ |
T10085 |
18324-18326 |
CD |
denotes |
30 |
T10086 |
18321-18323 |
CD |
denotes |
29 |
T10087 |
18323-18324 |
, |
denotes |
, |
T10088 |
18326-18327 |
-RRB- |
denotes |
] |
T10089 |
18339-18348 |
VBG |
denotes |
following |
T10090 |
18349-18356 |
NN |
denotes |
p150CAF |
T10091 |
18359-18368 |
NN |
denotes |
depletion |
T10092 |
18356-18357 |
HYPH |
denotes |
- |
T10093 |
18357-18358 |
CD |
denotes |
1 |
T10094 |
18369-18370 |
-LRB- |
denotes |
( |
T10095 |
18377-18379 |
NN |
denotes |
7A |
T10096 |
18370-18376 |
NN |
denotes |
Figure |
T10097 |
18380-18383 |
CC |
denotes |
and |
T10098 |
18384-18386 |
NN |
denotes |
7B |
T10099 |
18386-18387 |
-RRB- |
denotes |
) |
T10100 |
18387-18388 |
. |
denotes |
. |
T10101 |
18388-18585 |
sentence |
denotes |
Immunoprecipitation of minor satellite repeats in the same experiment was less affected (Figure 7B), showing that loss of p150CAF-1 function mainly affects H4K20me3 at pericentric heterochromatin. |
T10102 |
18389-18408 |
NN |
denotes |
Immunoprecipitation |
T10103 |
18468-18476 |
VBN |
denotes |
affected |
T10104 |
18409-18411 |
IN |
denotes |
of |
T10105 |
18412-18417 |
JJ |
denotes |
minor |
T10106 |
18428-18435 |
NNS |
denotes |
repeats |
T10107 |
18418-18427 |
NN |
denotes |
satellite |
T10108 |
18436-18438 |
IN |
denotes |
in |
T10109 |
18439-18442 |
DT |
denotes |
the |
T10110 |
18448-18458 |
NN |
denotes |
experiment |
T10111 |
18443-18447 |
JJ |
denotes |
same |
T10112 |
18459-18462 |
VBD |
denotes |
was |
T10113 |
18463-18467 |
RBR |
denotes |
less |
T10114 |
18477-18478 |
-LRB- |
denotes |
( |
T10115 |
18485-18487 |
NN |
denotes |
7B |
T10116 |
18478-18484 |
NN |
denotes |
Figure |
T10117 |
18487-18488 |
-RRB- |
denotes |
) |
T10118 |
18488-18490 |
, |
denotes |
, |
T10119 |
18490-18497 |
VBG |
denotes |
showing |
T10120 |
18498-18502 |
IN |
denotes |
that |
T10121 |
18537-18544 |
VBZ |
denotes |
affects |
T10122 |
18503-18507 |
NN |
denotes |
loss |
T10123 |
18508-18510 |
IN |
denotes |
of |
T10124 |
18511-18518 |
NN |
denotes |
p150CAF |
T10125 |
18521-18529 |
NN |
denotes |
function |
T10126 |
18518-18519 |
HYPH |
denotes |
- |
T10127 |
18519-18520 |
CD |
denotes |
1 |
T10128 |
18530-18536 |
RB |
denotes |
mainly |
T10129 |
18545-18553 |
NN |
denotes |
H4K20me3 |
T10130 |
18554-18556 |
IN |
denotes |
at |
T10131 |
18557-18568 |
JJ |
denotes |
pericentric |
T10132 |
18569-18584 |
NN |
denotes |
heterochromatin |
T10133 |
18584-18585 |
. |
denotes |
. |
T10134 |
18585-18804 |
sentence |
denotes |
Moreover, no significant difference in immunoprecipitation efficiency was detected at intracisternal A particle (IAP) elements (Figure 7B), which are noncentromeric repeated DNA elements also enriched in H4K20me3 [31]. |
T10135 |
18586-18594 |
RB |
denotes |
Moreover |
T10136 |
18660-18668 |
VBN |
denotes |
detected |
T10137 |
18594-18596 |
, |
denotes |
, |
T10138 |
18596-18598 |
DT |
denotes |
no |
T10139 |
18611-18621 |
NN |
denotes |
difference |
T10140 |
18599-18610 |
JJ |
denotes |
significant |
T10141 |
18622-18624 |
IN |
denotes |
in |
T10142 |
18625-18644 |
NN |
denotes |
immunoprecipitation |
T10143 |
18645-18655 |
NN |
denotes |
efficiency |
T10144 |
18656-18659 |
VBD |
denotes |
was |
T10145 |
18669-18671 |
IN |
denotes |
at |
T10146 |
18672-18686 |
JJ |
denotes |
intracisternal |
T10147 |
18689-18697 |
NN |
denotes |
particle |
T10148 |
18687-18688 |
NN |
denotes |
A |
T10149 |
18704-18712 |
NNS |
denotes |
elements |
T10150 |
18698-18699 |
-LRB- |
denotes |
( |
T10151 |
18699-18702 |
NN |
denotes |
IAP |
T10152 |
18702-18703 |
-RRB- |
denotes |
) |
T10153 |
18713-18714 |
-LRB- |
denotes |
( |
T10154 |
18721-18723 |
NN |
denotes |
7B |
T10155 |
18714-18720 |
NN |
denotes |
Figure |
T10156 |
18723-18724 |
-RRB- |
denotes |
) |
T10157 |
18724-18726 |
, |
denotes |
, |
T10158 |
18726-18731 |
WDT |
denotes |
which |
T10159 |
18732-18735 |
VBP |
denotes |
are |
T10160 |
18736-18750 |
JJ |
denotes |
noncentromeric |
T10161 |
18764-18772 |
NNS |
denotes |
elements |
T10162 |
18751-18759 |
VBN |
denotes |
repeated |
T10163 |
18760-18763 |
NN |
denotes |
DNA |
T10164 |
18773-18777 |
RB |
denotes |
also |
T10165 |
18778-18786 |
VBN |
denotes |
enriched |
T10166 |
18787-18789 |
IN |
denotes |
in |
T10167 |
18790-18798 |
NN |
denotes |
H4K20me3 |
T10168 |
18799-18800 |
-LRB- |
denotes |
[ |
T10169 |
18800-18802 |
CD |
denotes |
31 |
T10170 |
18802-18803 |
-RRB- |
denotes |
] |
T10171 |
18803-18804 |
. |
denotes |
. |
T10172 |
18804-18945 |
sentence |
denotes |
This result shows that p150CAF-1 contributes to normal levels of H4K20me3 at pericentric heterochromatin, but not necessarily at other loci. |
T10173 |
18805-18809 |
DT |
denotes |
This |
T10174 |
18810-18816 |
NN |
denotes |
result |
T10175 |
18817-18822 |
VBZ |
denotes |
shows |
T10176 |
18823-18827 |
IN |
denotes |
that |
T10177 |
18838-18849 |
VBZ |
denotes |
contributes |
T10178 |
18828-18835 |
NN |
denotes |
p150CAF |
T10179 |
18835-18836 |
HYPH |
denotes |
- |
T10180 |
18836-18837 |
CD |
denotes |
1 |
T10181 |
18850-18852 |
IN |
denotes |
to |
T10182 |
18853-18859 |
JJ |
denotes |
normal |
T10183 |
18860-18866 |
NNS |
denotes |
levels |
T10184 |
18867-18869 |
IN |
denotes |
of |
T10185 |
18870-18878 |
NN |
denotes |
H4K20me3 |
T10186 |
18879-18881 |
IN |
denotes |
at |
T10187 |
18882-18893 |
JJ |
denotes |
pericentric |
T10188 |
18894-18909 |
NN |
denotes |
heterochromatin |
T10189 |
18909-18911 |
, |
denotes |
, |
T10190 |
18911-18914 |
CC |
denotes |
but |
T10191 |
18915-18918 |
RB |
denotes |
not |
T10192 |
18919-18930 |
RB |
denotes |
necessarily |
T10193 |
18931-18933 |
IN |
denotes |
at |
T10194 |
18934-18939 |
JJ |
denotes |
other |
T10195 |
18940-18944 |
NNS |
denotes |
loci |
T10196 |
18944-18945 |
. |
denotes |
. |
T10197 |
18945-19087 |
sentence |
denotes |
The H3K9me3 mark [32] was also significantly reduced in p150CAF-1-depleted cells, though to a lesser extent than H4K20me3 (Figure 7A and 7B). |
T10198 |
18946-18949 |
DT |
denotes |
The |
T10199 |
18958-18962 |
NN |
denotes |
mark |
T10200 |
18950-18957 |
NN |
denotes |
H3K9me3 |
T10201 |
18991-18998 |
VBN |
denotes |
reduced |
T10202 |
18963-18964 |
-LRB- |
denotes |
[ |
T10203 |
18964-18966 |
CD |
denotes |
32 |
T10204 |
18966-18967 |
-RRB- |
denotes |
] |
T10205 |
18968-18971 |
VBD |
denotes |
was |
T10206 |
18972-18976 |
RB |
denotes |
also |
T10207 |
18977-18990 |
RB |
denotes |
significantly |
T10208 |
18999-19001 |
IN |
denotes |
in |
T10209 |
19002-19009 |
NN |
denotes |
p150CAF |
T10210 |
19012-19020 |
VBN |
denotes |
depleted |
T10211 |
19009-19010 |
HYPH |
denotes |
- |
T10212 |
19010-19011 |
CD |
denotes |
1 |
T10213 |
19011-19012 |
HYPH |
denotes |
- |
T10214 |
19021-19026 |
NNS |
denotes |
cells |
T10215 |
19026-19028 |
, |
denotes |
, |
T10216 |
19028-19034 |
IN |
denotes |
though |
T10217 |
19035-19037 |
IN |
denotes |
to |
T10218 |
19038-19039 |
DT |
denotes |
a |
T10219 |
19047-19053 |
NN |
denotes |
extent |
T10220 |
19040-19046 |
JJR |
denotes |
lesser |
T10221 |
19054-19058 |
IN |
denotes |
than |
T10222 |
19059-19067 |
NN |
denotes |
H4K20me3 |
T10223 |
19068-19069 |
-LRB- |
denotes |
( |
T10224 |
19076-19078 |
NN |
denotes |
7A |
T10225 |
19069-19075 |
NN |
denotes |
Figure |
T10226 |
19079-19082 |
CC |
denotes |
and |
T10227 |
19083-19085 |
NN |
denotes |
7B |
T10228 |
19085-19086 |
-RRB- |
denotes |
) |
T10229 |
19086-19087 |
. |
denotes |
. |
T10230 |
19087-20985 |
sentence |
denotes |
Figure 6 DNA CpG Methylation at Pericentric Heterochromatin Is Not Altered in p150CAF-1-Depleted ES Cells
Total DNA was isolated from cells transfected with a control or the p150CAF-1 siRNA vector and digested with MaeII, whose recognition sequence is present in major satellite repeats. Digested DNA was run onto an agarose gel and analyzed by Southern blotting using the pSAT major satellite probe [47].
Figure 7 Alteration of Epigenetic Marking at Pericentric Heterochromatin in p150CAF-1-Depleted Cells
(A) p150CAF-1 depletion leads to reduced H4K20me3 and H3K9me3 at pericentric heterochromatin. Enrichment of histone marks at major satellite repeats was determined by ChIP from control (cont) and p150CAF-1 (p150) siRNA-expressing ES cells. DNA prepared from the input and the antibody-bound fraction were run onto an agarose gel and analyzed by Southern blot with the pSAT major satellite repeat probe [47].
(B) Hybridization signals were quantified using an Instant Imager. After autoradiography, the membrane was stripped and rehybridized with a minor satellite probe [49]. After quantification, the membrane was stripped and rehybridized with an IAP LTR probe [50]. Results are presented as the amount of DNA immunoprecipitated from p150CAF-1-depleted ES cells divided by the DNA obtained from control cells. The figure shows the mean value and standard deviation of three independent ChIP experiments.
(C and D) H3K9me3 and H4K20me3 fluorescence patterns are severely altered in p150CAF-1-depleted ES cells. Immunodetection of H3K9me3 (C, green), H4K20me3 (D, green), and HP1α (red) in control and p150CAF-1 siRNA-expressing ES cells. Merging of HP1α with H3K9me3 (C) and H4K20me3 (D) is shown in yellow. Scale bars represent 10 μm. In wild-type cells, most of H4K20me3 and H3K9me3 signals are present at the level of DAPI-dense domains when visualized by IF [29] (Figures 7C and 7D). |
T10231 |
20834-20836 |
IN |
denotes |
In |
T10232 |
20891-20894 |
VBP |
denotes |
are |
T10233 |
20837-20841 |
JJ |
denotes |
wild |
T10234 |
20842-20846 |
NN |
denotes |
type |
T10235 |
20841-20842 |
HYPH |
denotes |
- |
T10236 |
20847-20852 |
NNS |
denotes |
cells |
T10237 |
20852-20854 |
, |
denotes |
, |
T10238 |
20854-20858 |
JJS |
denotes |
most |
T10239 |
20859-20861 |
IN |
denotes |
of |
T10240 |
20862-20870 |
NN |
denotes |
H4K20me3 |
T10241 |
20883-20890 |
NNS |
denotes |
signals |
T10242 |
20871-20874 |
CC |
denotes |
and |
T10243 |
20875-20882 |
NN |
denotes |
H3K9me3 |
T10244 |
20895-20902 |
JJ |
denotes |
present |
T10245 |
20903-20905 |
IN |
denotes |
at |
T10246 |
20906-20909 |
DT |
denotes |
the |
T10247 |
20910-20915 |
NN |
denotes |
level |
T10248 |
20916-20918 |
IN |
denotes |
of |
T10249 |
20919-20923 |
NN |
denotes |
DAPI |
T10250 |
20924-20929 |
JJ |
denotes |
dense |
T10251 |
20923-20924 |
HYPH |
denotes |
- |
T10252 |
20930-20937 |
NNS |
denotes |
domains |
T10253 |
20938-20942 |
WRB |
denotes |
when |
T10254 |
20943-20953 |
VBN |
denotes |
visualized |
T10255 |
20954-20956 |
IN |
denotes |
by |
T10256 |
20957-20959 |
NN |
denotes |
IF |
T10257 |
20960-20961 |
-LRB- |
denotes |
[ |
T10258 |
20961-20963 |
CD |
denotes |
29 |
T10259 |
20963-20964 |
-RRB- |
denotes |
] |
T10260 |
20965-20966 |
-LRB- |
denotes |
( |
T10261 |
20974-20976 |
NN |
denotes |
7C |
T10262 |
20966-20973 |
NNS |
denotes |
Figures |
T10263 |
20977-20980 |
CC |
denotes |
and |
T10264 |
20981-20983 |
NN |
denotes |
7D |
T10265 |
20983-20984 |
-RRB- |
denotes |
) |
T10266 |
20984-20985 |
. |
denotes |
. |
T10267 |
20985-21103 |
sentence |
denotes |
H3K9me3 was severely perturbed in p150CAF-1-depleted cells, displaying a diffuse pattern similar to HP1α (Figure 7C). |
T10268 |
20986-20993 |
NN |
denotes |
H3K9me3 |
T10269 |
21007-21016 |
VBN |
denotes |
perturbed |
T10270 |
20994-20997 |
VBD |
denotes |
was |
T10271 |
20998-21006 |
RB |
denotes |
severely |
T10272 |
21017-21019 |
IN |
denotes |
in |
T10273 |
21020-21027 |
NN |
denotes |
p150CAF |
T10274 |
21030-21038 |
VBN |
denotes |
depleted |
T10275 |
21027-21028 |
HYPH |
denotes |
- |
T10276 |
21028-21029 |
CD |
denotes |
1 |
T10277 |
21029-21030 |
HYPH |
denotes |
- |
T10278 |
21039-21044 |
NNS |
denotes |
cells |
T10279 |
21044-21046 |
, |
denotes |
, |
T10280 |
21046-21056 |
VBG |
denotes |
displaying |
T10281 |
21057-21058 |
DT |
denotes |
a |
T10282 |
21067-21074 |
NN |
denotes |
pattern |
T10283 |
21059-21066 |
JJ |
denotes |
diffuse |
T10284 |
21075-21082 |
JJ |
denotes |
similar |
T10285 |
21083-21085 |
IN |
denotes |
to |
T10286 |
21086-21090 |
NN |
denotes |
HP1α |
T10287 |
21091-21092 |
-LRB- |
denotes |
( |
T10288 |
21099-21101 |
NN |
denotes |
7C |
T10289 |
21092-21098 |
NN |
denotes |
Figure |
T10290 |
21101-21102 |
-RRB- |
denotes |
) |
T10291 |
21102-21103 |
. |
denotes |
. |
T10292 |
21103-21271 |
sentence |
denotes |
The H4K20me3 pattern was even more severely altered, showing both a loss of the typical dots revealed in control cells and a reduction in signal intensity (Figure 7D). |
T10293 |
21104-21107 |
DT |
denotes |
The |
T10294 |
21117-21124 |
NN |
denotes |
pattern |
T10295 |
21108-21116 |
NN |
denotes |
H4K20me3 |
T10296 |
21148-21155 |
VBN |
denotes |
altered |
T10297 |
21125-21128 |
VBD |
denotes |
was |
T10298 |
21129-21133 |
RB |
denotes |
even |
T10299 |
21134-21138 |
RBR |
denotes |
more |
T10300 |
21139-21147 |
RB |
denotes |
severely |
T10301 |
21155-21157 |
, |
denotes |
, |
T10302 |
21157-21164 |
VBG |
denotes |
showing |
T10303 |
21165-21169 |
CC |
denotes |
both |
T10304 |
21172-21176 |
NN |
denotes |
loss |
T10305 |
21170-21171 |
DT |
denotes |
a |
T10306 |
21177-21179 |
IN |
denotes |
of |
T10307 |
21180-21183 |
DT |
denotes |
the |
T10308 |
21192-21196 |
NNS |
denotes |
dots |
T10309 |
21184-21191 |
JJ |
denotes |
typical |
T10310 |
21197-21205 |
VBN |
denotes |
revealed |
T10311 |
21206-21208 |
IN |
denotes |
in |
T10312 |
21209-21216 |
NN |
denotes |
control |
T10313 |
21217-21222 |
NNS |
denotes |
cells |
T10314 |
21223-21226 |
CC |
denotes |
and |
T10315 |
21227-21228 |
DT |
denotes |
a |
T10316 |
21229-21238 |
NN |
denotes |
reduction |
T10317 |
21239-21241 |
IN |
denotes |
in |
T10318 |
21242-21248 |
NN |
denotes |
signal |
T10319 |
21249-21258 |
NN |
denotes |
intensity |
T10320 |
21259-21260 |
-LRB- |
denotes |
( |
T10321 |
21267-21269 |
NN |
denotes |
7D |
T10322 |
21260-21266 |
NN |
denotes |
Figure |
T10323 |
21269-21270 |
-RRB- |
denotes |
) |
T10324 |
21270-21271 |
. |
denotes |
. |
T10325 |
21271-21397 |
sentence |
denotes |
Consistent with our ChIP experiments, residual H4K20me3 epitopes were detected at the level of relocated DAPI-dense material. |
T10326 |
21272-21282 |
JJ |
denotes |
Consistent |
T10327 |
21342-21350 |
VBN |
denotes |
detected |
T10328 |
21283-21287 |
IN |
denotes |
with |
T10329 |
21288-21291 |
PRP$ |
denotes |
our |
T10330 |
21297-21308 |
NNS |
denotes |
experiments |
T10331 |
21292-21296 |
NN |
denotes |
ChIP |
T10332 |
21308-21310 |
, |
denotes |
, |
T10333 |
21310-21318 |
JJ |
denotes |
residual |
T10334 |
21328-21336 |
NNS |
denotes |
epitopes |
T10335 |
21319-21327 |
NN |
denotes |
H4K20me3 |
T10336 |
21337-21341 |
VBD |
denotes |
were |
T10337 |
21351-21353 |
IN |
denotes |
at |
T10338 |
21354-21357 |
DT |
denotes |
the |
T10339 |
21358-21363 |
NN |
denotes |
level |
T10340 |
21364-21366 |
IN |
denotes |
of |
T10341 |
21367-21376 |
VBN |
denotes |
relocated |
T10342 |
21388-21396 |
NN |
denotes |
material |
T10343 |
21377-21381 |
NN |
denotes |
DAPI |
T10344 |
21382-21387 |
JJ |
denotes |
dense |
T10345 |
21381-21382 |
HYPH |
denotes |
- |
T10346 |
21396-21397 |
. |
denotes |
. |
T10347 |
21397-21574 |
sentence |
denotes |
Altogether, our results show that depletion of CAF-1 in ES cells leads to a simultaneous disruption of heterochromatin 3-D organization and an alteration in epigenetic marking. |
T10348 |
21398-21408 |
RB |
denotes |
Altogether |
T10349 |
21422-21426 |
VBP |
denotes |
show |
T10350 |
21408-21410 |
, |
denotes |
, |
T10351 |
21410-21413 |
PRP$ |
denotes |
our |
T10352 |
21414-21421 |
NNS |
denotes |
results |
T10353 |
21427-21431 |
IN |
denotes |
that |
T10354 |
21463-21468 |
VBZ |
denotes |
leads |
T10355 |
21432-21441 |
NN |
denotes |
depletion |
T10356 |
21442-21444 |
IN |
denotes |
of |
T10357 |
21445-21448 |
NN |
denotes |
CAF |
T10358 |
21448-21449 |
HYPH |
denotes |
- |
T10359 |
21449-21450 |
CD |
denotes |
1 |
T10360 |
21451-21453 |
IN |
denotes |
in |
T10361 |
21454-21456 |
NN |
denotes |
ES |
T10362 |
21457-21462 |
NNS |
denotes |
cells |
T10363 |
21469-21471 |
IN |
denotes |
to |
T10364 |
21472-21473 |
DT |
denotes |
a |
T10365 |
21487-21497 |
NN |
denotes |
disruption |
T10366 |
21474-21486 |
JJ |
denotes |
simultaneous |
T10367 |
21498-21500 |
IN |
denotes |
of |
T10368 |
21501-21516 |
NN |
denotes |
heterochromatin |
T10369 |
21521-21533 |
NN |
denotes |
organization |
T10370 |
21517-21518 |
CD |
denotes |
3 |
T10371 |
21519-21520 |
NN |
denotes |
D |
T10372 |
21518-21519 |
HYPH |
denotes |
- |
T10373 |
21534-21537 |
CC |
denotes |
and |
T10374 |
21538-21540 |
DT |
denotes |
an |
T10375 |
21541-21551 |
NN |
denotes |
alteration |
T10376 |
21552-21554 |
IN |
denotes |
in |
T10377 |
21555-21565 |
JJ |
denotes |
epigenetic |
T10378 |
21566-21573 |
NN |
denotes |
marking |
T10379 |
21573-21574 |
. |
denotes |
. |
T8538 |
5453-5455 |
CD |
denotes |
16 |
R795 |
T7971 |
T7972 |
nsubj |
We,used |
R805 |
T7973 |
T7974 |
compound |
gene,targeting |
R806 |
T7974 |
T7972 |
dobj |
targeting,used |
R807 |
T7975 |
T7972 |
prep |
in,used |
R808 |
T7976 |
T7977 |
compound |
ES,cells |
R809 |
T7977 |
T7975 |
pobj |
cells,in |
R810 |
T7978 |
T7979 |
aux |
to,delete |
R811 |
T8011 |
T8010 |
prep |
of,were |
R812 |
T7979 |
T7972 |
advcl |
delete,used |
R813 |
T8012 |
T8013 |
amod |
normal,size |
R814 |
T8013 |
T8011 |
pobj |
size,of |
R815 |
T8014 |
T8013 |
cc |
and,size |
R816 |
T8015 |
T8013 |
conj |
weight,size |
R817 |
T7980 |
T7979 |
dobj |
exon,delete |
R818 |
T8016 |
T8010 |
punct |
", ",were |
R819 |
T8017 |
T8010 |
conj |
displayed,were |
R820 |
T8018 |
T8019 |
det |
no,abnormalities |
R821 |
T8019 |
T8017 |
dobj |
abnormalities,displayed |
R822 |
T7981 |
T7980 |
nummod |
3,exon |
R823 |
T8020 |
T8019 |
amod |
obvious,abnormalities |
R824 |
T7982 |
T7979 |
prep |
in,delete |
R825 |
T8021 |
T8017 |
punct |
", ",displayed |
R826 |
T8022 |
T8017 |
cc |
and,displayed |
R827 |
T8023 |
T8017 |
conj |
were,displayed |
R828 |
T8024 |
T8023 |
acomp |
fertile,were |
R829 |
T8025 |
T8003 |
punct |
.,born |
R830 |
T7983 |
T7984 |
det |
the,gene |
R831 |
T8027 |
T8028 |
csubj |
Crossing,failed |
R832 |
T8029 |
T8030 |
amod |
heterozygous,mice |
R833 |
T8030 |
T8027 |
dobj |
mice,Crossing |
R834 |
T8031 |
T8032 |
aux |
to,generate |
R835 |
T8032 |
T8028 |
xcomp |
generate,failed |
R836 |
T8033 |
T8034 |
amod |
viable,mice |
R837 |
T8034 |
T8032 |
dobj |
mice,generate |
R838 |
T8035 |
T8034 |
amod |
newborn,mice |
R839 |
T8036 |
T8034 |
nmod |
Chaf1a,mice |
R840 |
T7984 |
T7982 |
pobj |
gene,in |
R841 |
T8037 |
T8036 |
punct |
−,Chaf1a |
R842 |
T8038 |
T8036 |
punct |
/,Chaf1a |
R843 |
T8039 |
T8036 |
punct |
−,Chaf1a |
R844 |
T8040 |
T8028 |
punct |
.,failed |
R845 |
T7985 |
T7984 |
compound |
Chaf1a,gene |
R846 |
T8042 |
T8043 |
advmod |
Furthermore,detected |
R847 |
T7986 |
T7984 |
punct |
", ",gene |
R848 |
T8044 |
T8043 |
punct |
", ",detected |
R849 |
T8045 |
T8046 |
det |
no,embryos |
R850 |
T8046 |
T8043 |
nsubjpass |
embryos,detected |
R851 |
T8047 |
T8046 |
amod |
homozygous,embryos |
R852 |
T7987 |
T7988 |
dep |
which,encodes |
R853 |
T8048 |
T8046 |
nmod |
Chaf1a,embryos |
R854 |
T8049 |
T8048 |
punct |
−,Chaf1a |
R855 |
T8050 |
T8048 |
punct |
/,Chaf1a |
R856 |
T7988 |
T7984 |
relcl |
encodes,gene |
R857 |
T8051 |
T8048 |
punct |
−,Chaf1a |
R858 |
T8052 |
T8043 |
auxpass |
were,detected |
R859 |
T8053 |
T8043 |
prep |
at,detected |
R860 |
T7989 |
T7988 |
dobj |
p150CAF,encodes |
R861 |
T8054 |
T8053 |
pobj |
any,at |
R862 |
T8055 |
T8054 |
prep |
of,any |
R863 |
T8056 |
T8057 |
det |
the,stages |
R864 |
T7990 |
T7989 |
punct |
-,p150CAF |
R865 |
T8057 |
T8055 |
pobj |
stages,of |
R866 |
T8058 |
T8057 |
amod |
post-implantation,stages |
R867 |
T8059 |
T8043 |
punct |
.,detected |
R868 |
T7991 |
T7989 |
nummod |
1,p150CAF |
R869 |
T8061 |
T8062 |
advmod |
However,detect |
R870 |
T7992 |
T7993 |
punct |
(,1A |
R871 |
T8063 |
T8062 |
punct |
", ",detect |
R872 |
T8064 |
T8062 |
nsubj |
we,detect |
R873 |
T8065 |
T8062 |
aux |
could,detect |
R874 |
T7993 |
T7972 |
parataxis |
1A,used |
R875 |
T8066 |
T8067 |
nmod |
Chaf1a,embryos |
R876 |
T8067 |
T8062 |
dobj |
embryos,detect |
R877 |
T8068 |
T8066 |
punct |
−,Chaf1a |
R878 |
T7994 |
T7993 |
compound |
Figure,1A |
R879 |
T8069 |
T8066 |
punct |
/,Chaf1a |
R880 |
T8070 |
T8066 |
punct |
−,Chaf1a |
R881 |
T8071 |
T8062 |
prep |
at,detect |
R882 |
T8072 |
T8073 |
amod |
embryonic,day |
R883 |
T8073 |
T8071 |
pobj |
day,at |
R884 |
T8074 |
T8073 |
nummod |
4,day |
R885 |
T8075 |
T8073 |
punct |
(,day |
R886 |
T7995 |
T7993 |
punct |
),1A |
R887 |
T8076 |
T8073 |
appos |
E4,day |
R888 |
T7996 |
T7972 |
punct |
.,used |
R889 |
T8077 |
T8062 |
punct |
),detect |
R890 |
T8078 |
T8062 |
advcl |
using,detect |
R891 |
T7998 |
T7999 |
nmod |
Chaf1a,mice |
R892 |
T8079 |
T8080 |
det |
a,strategy |
R893 |
T8080 |
T8078 |
dobj |
strategy,using |
R894 |
T8081 |
T8080 |
compound |
PCR,strategy |
R895 |
T8082 |
T8083 |
punct |
(,1B |
R896 |
T7999 |
T8003 |
nsubjpass |
mice,born |
R897 |
T8083 |
T8062 |
parataxis |
1B,detect |
R898 |
T8084 |
T8083 |
compound |
Figure,1B |
R899 |
T8085 |
T8083 |
punct |
),1B |
R900 |
T8000 |
T7998 |
punct |
+,Chaf1a |
R901 |
T8086 |
T8062 |
punct |
.,detect |
R902 |
T8088 |
T8089 |
nsubj |
They,represent |
R903 |
T8001 |
T7998 |
punct |
/,Chaf1a |
R904 |
T8090 |
T8091 |
advmod |
only,10 |
R905 |
T8091 |
T8092 |
nummod |
10,% |
R906 |
T8002 |
T7998 |
punct |
−,Chaf1a |
R907 |
T8092 |
T8089 |
dobj |
%,represent |
R908 |
T8093 |
T8092 |
prep |
of,% |
R909 |
T8094 |
T8095 |
compound |
E4,embryos |
R910 |
T8004 |
T8003 |
auxpass |
were,born |
R911 |
T8095 |
T8093 |
pobj |
embryos,of |
R912 |
T8096 |
T8092 |
acl |
obtained,% |
R913 |
T8097 |
T8096 |
prep |
from,obtained |
R914 |
T8098 |
T8099 |
nmod |
Chaf1a,intercrosses |
R915 |
T8005 |
T8003 |
prep |
at,born |
R916 |
T8099 |
T8097 |
pobj |
intercrosses,from |
R917 |
T8100 |
T8098 |
punct |
+,Chaf1a |
R918 |
T8101 |
T8098 |
punct |
/,Chaf1a |
R919 |
T8006 |
T8007 |
det |
a,frequency |
R920 |
T8102 |
T8098 |
punct |
−,Chaf1a |
R921 |
T8103 |
T8099 |
compound |
mice,intercrosses |
R922 |
T8104 |
T8089 |
punct |
", ",represent |
R923 |
T8105 |
T8089 |
advcl |
suggesting,represent |
R924 |
T8007 |
T8005 |
pobj |
frequency,at |
R925 |
T8106 |
T8107 |
mark |
that,degenerated |
R926 |
T8107 |
T8105 |
ccomp |
degenerated,suggesting |
R927 |
T8008 |
T8007 |
amod |
Mendelian,frequency |
R928 |
T8108 |
T8109 |
amod |
more,half |
R929 |
T8109 |
T8107 |
nsubj |
half,degenerated |
R930 |
T8110 |
T8109 |
quantmod |
than,half |
R931 |
T8111 |
T8109 |
prep |
of,half |
R932 |
T8009 |
T8003 |
punct |
", ",born |
R933 |
T8112 |
T8113 |
det |
the,embryos |
R934 |
T8113 |
T8111 |
pobj |
embryos,of |
R935 |
T8114 |
T8115 |
amod |
homozygous,mutant |
R936 |
T8115 |
T8113 |
compound |
mutant,embryos |
R937 |
T8116 |
T8107 |
aux |
had,degenerated |
R938 |
T8010 |
T8003 |
conj |
were,born |
R939 |
T8223 |
T8224 |
advmod |
Thus,allows |
R940 |
T8117 |
T8107 |
prep |
before,degenerated |
R941 |
T8118 |
T8119 |
det |
this,stage |
R942 |
T8119 |
T8117 |
pobj |
stage,before |
R943 |
T8120 |
T8089 |
punct |
.,represent |
R944 |
T8225 |
T8224 |
punct |
", ",allows |
R945 |
T8122 |
T8123 |
advmod |
Moreover,contained |
R946 |
T8124 |
T8123 |
punct |
", ",contained |
R947 |
T8125 |
T8126 |
compound |
mutant,embryos |
R948 |
T8126 |
T8123 |
nsubj |
embryos,contained |
R949 |
T8226 |
T8224 |
nsubj |
depletion,allows |
R950 |
T8127 |
T8128 |
advmod |
only,16 |
R951 |
T8128 |
T8131 |
nummod |
16,cells |
R952 |
T8129 |
T8128 |
quantmod |
eight,16 |
R953 |
T8227 |
T8226 |
prep |
of,depletion |
R954 |
T8130 |
T8128 |
quantmod |
to,16 |
R955 |
T8131 |
T8123 |
dobj |
cells,contained |
R956 |
T8132 |
T8123 |
prep |
at,contained |
R957 |
T8228 |
T8229 |
nmod |
p150CAF,protein |
R958 |
T8133 |
T8134 |
det |
the,stage |
R959 |
T8134 |
T8132 |
pobj |
stage,at |
R960 |
T8135 |
T8134 |
compound |
E4,stage |
R961 |
T8229 |
T8227 |
pobj |
protein,of |
R962 |
T8136 |
T8123 |
punct |
", ",contained |
R963 |
T8137 |
T8138 |
advmod |
instead,of |
R964 |
T8138 |
T8123 |
prep |
of,contained |
R965 |
T8230 |
T8228 |
punct |
-,p150CAF |
R966 |
T8139 |
T8140 |
nummod |
32,cells |
R967 |
T8140 |
T8138 |
pobj |
cells,of |
R968 |
T8141 |
T8140 |
acl |
observed,cells |
R969 |
T8231 |
T8228 |
nummod |
1,p150CAF |
R970 |
T8142 |
T8141 |
prep |
for,observed |
R971 |
T8143 |
T8144 |
amod |
wild,type |
R972 |
T8144 |
T8146 |
nmod |
type,blastocysts |
R973 |
T8232 |
T8226 |
prep |
from,depletion |
R974 |
T8145 |
T8144 |
punct |
-,type |
R975 |
T8146 |
T8142 |
pobj |
blastocysts,for |
R976 |
T8147 |
T8144 |
cc |
or,type |
R977 |
T8233 |
T8234 |
det |
this,stage |
R978 |
T8148 |
T8144 |
conj |
heterozygous,type |
R979 |
T8234 |
T8232 |
pobj |
stage,from |
R980 |
T8149 |
T8150 |
punct |
(,1C |
R981 |
T8150 |
T8123 |
parataxis |
1C,contained |
R982 |
T8151 |
T8150 |
compound |
Figure,1C |
R983 |
T8152 |
T8150 |
cc |
and,1C |
R984 |
T8235 |
T8236 |
det |
a,maximum |
R985 |
T8153 |
T8150 |
conj |
1D,1C |
R986 |
T8154 |
T8150 |
punct |
),1C |
R987 |
T8155 |
T8123 |
punct |
.,contained |
R988 |
T8157 |
T8158 |
amod |
Further,analysis |
R989 |
T8158 |
T8159 |
nsubj |
analysis,allowed |
R990 |
T8236 |
T8224 |
dobj |
maximum,allows |
R991 |
T8160 |
T8158 |
prep |
by,analysis |
R992 |
T8161 |
T8162 |
compound |
light,microscopy |
R993 |
T8237 |
T8236 |
prep |
of,maximum |
R994 |
T8162 |
T8160 |
pobj |
microscopy,by |
R995 |
T8238 |
T8239 |
nummod |
two,divisions |
R996 |
T8163 |
T8162 |
acl |
using,microscopy |
R997 |
T8164 |
T8163 |
dobj |
immunofluorescence,using |
R998 |
T8239 |
T8237 |
pobj |
divisions,of |
R999 |
T8165 |
T8166 |
punct |
(,IF |
R1000 |
T8166 |
T8164 |
parataxis |
IF,immunofluorescence |
R1001 |
T8167 |
T8166 |
punct |
),IF |
R1002 |
T8240 |
T8239 |
amod |
additional,divisions |
R1003 |
T8168 |
T8169 |
nsubj |
us,compare |
R1004 |
T8169 |
T8159 |
ccomp |
compare,allowed |
R1005 |
T8170 |
T8169 |
aux |
to,compare |
R1006 |
T8241 |
T8239 |
compound |
cell,divisions |
R1007 |
T8171 |
T8172 |
amod |
wild,type |
R1008 |
T8172 |
T8174 |
nmod |
type,embryos |
R1009 |
T8173 |
T8172 |
punct |
-,type |
R1010 |
T8242 |
T8224 |
prep |
before,allows |
R1011 |
T8174 |
T8169 |
dobj |
embryos,compare |
R1012 |
T8175 |
T8172 |
cc |
and,type |
R1013 |
T8176 |
T8177 |
npadvmod |
p150CAF,depleted |
R1014 |
T8177 |
T8172 |
conj |
depleted,type |
R1015 |
T8243 |
T8244 |
amod |
developmental,arrest |
R1016 |
T8244 |
T8242 |
pobj |
arrest,before |
R1017 |
T8178 |
T8176 |
punct |
-,p150CAF |
R1018 |
T8245 |
T8224 |
punct |
.,allows |
R1019 |
T8179 |
T8176 |
nummod |
1,p150CAF |
R1020 |
T8180 |
T8177 |
punct |
-,depleted |
R1021 |
T8247 |
T8248 |
advmod |
Strikingly,found |
R1022 |
T8181 |
T8159 |
punct |
.,allowed |
R1023 |
T8183 |
T8184 |
det |
A,loss |
R1024 |
T8184 |
T8185 |
nsubjpass |
loss,detected |
R1025 |
T8249 |
T8248 |
punct |
", ",found |
R1026 |
T8186 |
T8184 |
prep |
of,loss |
R1027 |
T8187 |
T8188 |
nmod |
p150CAF,staining |
R1028 |
T8188 |
T8186 |
pobj |
staining,of |
R1029 |
T8189 |
T8187 |
punct |
-,p150CAF |
R1030 |
T8250 |
T8248 |
nsubj |
we,found |
R1031 |
T8190 |
T8187 |
nummod |
1,p150CAF |
R1032 |
T8191 |
T8185 |
aux |
could,detected |
R1033 |
T8251 |
T8252 |
mark |
that,appeared |
R1034 |
T8192 |
T8185 |
neg |
not,detected |
R1035 |
T8193 |
T8185 |
auxpass |
be,detected |
R1036 |
T8194 |
T8185 |
prep |
before,detected |
R1037 |
T8195 |
T8196 |
det |
the,stage |
R1038 |
T8252 |
T8248 |
ccomp |
appeared,found |
R1039 |
T8196 |
T8194 |
pobj |
stage,before |
R1040 |
T8197 |
T8198 |
nummod |
eight,cell |
R1041 |
T8198 |
T8196 |
compound |
cell,stage |
R1042 |
T8199 |
T8198 |
punct |
-,cell |
R1043 |
T8200 |
T8201 |
punct |
(,data |
R1044 |
T8201 |
T8185 |
meta |
data,detected |
R1045 |
T8253 |
T8254 |
det |
the,organization |
R1046 |
T8202 |
T8201 |
amod |
unpublished,data |
R1047 |
T8203 |
T8201 |
punct |
),data |
R1048 |
T8204 |
T8185 |
advcl |
suggesting,detected |
R1049 |
T8254 |
T8252 |
nsubj |
organization,appeared |
R1050 |
T8205 |
T8206 |
mark |
that,is |
R1051 |
T8206 |
T8204 |
ccomp |
is,suggesting |
R1052 |
T8207 |
T8208 |
advmod |
maternally,contributed |
R1053 |
T8255 |
T8254 |
amod |
nuclear,organization |
R1054 |
T8208 |
T8209 |
amod |
contributed,protein |
R1055 |
T8209 |
T8206 |
nsubj |
protein,is |
R1056 |
T8210 |
T8206 |
acomp |
present,is |
R1057 |
T8256 |
T8254 |
prep |
of,organization |
R1058 |
T8211 |
T8206 |
prep |
in,is |
R1059 |
T8212 |
T8213 |
det |
the,embryo |
R1060 |
T8213 |
T8211 |
pobj |
embryo,in |
R1061 |
T8257 |
T8256 |
pobj |
heterochromatin,of |
R1062 |
T8214 |
T8206 |
prep |
up,is |
R1063 |
T8215 |
T8214 |
prep |
to,up |
R1064 |
T8216 |
T8217 |
det |
the,stage |
R1065 |
T8258 |
T8252 |
oprd |
abnormal,appeared |
R1066 |
T8217 |
T8215 |
pobj |
stage,to |
R1067 |
T8218 |
T8219 |
nummod |
four,cell |
R1068 |
T8219 |
T8217 |
compound |
cell,stage |
R1069 |
T8259 |
T8252 |
prep |
in,appeared |
R1070 |
T8220 |
T8219 |
punct |
-,cell |
R1071 |
T8221 |
T8185 |
punct |
.,detected |
R1072 |
T8260 |
T8261 |
det |
the,nuclei |
R1073 |
T8261 |
T8259 |
pobj |
nuclei,in |
R1074 |
T8262 |
T8261 |
prep |
of,nuclei |
R1075 |
T8328 |
T8326 |
acl |
called,structures |
R1076 |
T8329 |
T8328 |
oprd |
chromocenters,called |
R1077 |
T8263 |
T8264 |
nmod |
Chaf1a,embryos |
R1078 |
T8330 |
T8331 |
punct |
[,20 |
R1079 |
T8331 |
T8328 |
parataxis |
20,called |
R1080 |
T8332 |
T8331 |
punct |
],20 |
R1081 |
T8264 |
T8262 |
pobj |
embryos,of |
R1082 |
T8333 |
T8326 |
punct |
", ",structures |
R1083 |
T8334 |
T8335 |
dep |
which,visualized |
R1084 |
T8265 |
T8263 |
punct |
−,Chaf1a |
R1085 |
T8335 |
T8326 |
relcl |
visualized,structures |
R1086 |
T8266 |
T8263 |
punct |
/,Chaf1a |
R1087 |
T8336 |
T8335 |
auxpass |
are,visualized |
R1088 |
T8267 |
T8263 |
punct |
−,Chaf1a |
R1089 |
T8337 |
T8335 |
prep |
as,visualized |
R1090 |
T8338 |
T8339 |
npadvmod |
DAPI,dense |
R1091 |
T8339 |
T8341 |
amod |
dense,foci |
R1092 |
T8340 |
T8339 |
punct |
-,dense |
R1093 |
T8341 |
T8337 |
pobj |
foci,as |
R1094 |
T8342 |
T8343 |
punct |
(,1C |
R1095 |
T8268 |
T8248 |
punct |
.,found |
R1096 |
T8343 |
T8322 |
parataxis |
1C,form |
R1097 |
T8344 |
T8343 |
compound |
Figure,1C |
R1098 |
T8345 |
T8343 |
punct |
),1C |
R1099 |
T8270 |
T8271 |
amod |
Pericentric,heterochromatin |
R1100 |
T8346 |
T8306 |
punct |
.,cluster |
R1101 |
T8348 |
T8349 |
det |
These,structures |
R1102 |
T8349 |
T8350 |
nsubjpass |
structures,detected |
R1103 |
T8271 |
T8272 |
nsubjpass |
heterochromatin,visualized |
R1104 |
T8351 |
T8350 |
auxpass |
were,detected |
R1105 |
T8352 |
T8350 |
neg |
not,detected |
R1106 |
T8273 |
T8271 |
punct |
", ",heterochromatin |
R1107 |
T8353 |
T8350 |
prep |
in,detected |
R1108 |
T8354 |
T8355 |
nmod |
Chaf1a,embryos |
R1109 |
T8355 |
T8353 |
pobj |
embryos,in |
R1110 |
T8356 |
T8354 |
punct |
−,Chaf1a |
R1111 |
T8274 |
T8275 |
det |
the,component |
R1112 |
T8357 |
T8354 |
punct |
/,Chaf1a |
R1113 |
T8358 |
T8354 |
punct |
−,Chaf1a |
R1114 |
T8359 |
T8360 |
punct |
(,1D |
R1115 |
T8275 |
T8271 |
appos |
component,heterochromatin |
R1116 |
T8360 |
T8350 |
parataxis |
1D,detected |
R1117 |
T8361 |
T8360 |
compound |
Figure,1D |
R1118 |
T8362 |
T8360 |
punct |
),1D |
R1119 |
T8276 |
T8275 |
amod |
major,component |
R1120 |
T8363 |
T8350 |
punct |
.,detected |
R1121 |
T8365 |
T8366 |
mark |
Since,are |
R1122 |
T8277 |
T8275 |
prep |
of,component |
R1123 |
T8366 |
T8374 |
advcl |
are,decided |
R1124 |
T8367 |
T8368 |
compound |
chromatin,architecture |
R1125 |
T8368 |
T8366 |
nsubj |
architecture,are |
R1126 |
T8278 |
T8279 |
amod |
constitutive,heterochromatin |
R1127 |
T8369 |
T8368 |
cc |
and,architecture |
R1128 |
T8370 |
T8371 |
amod |
higher,order |
R1129 |
T8371 |
T8373 |
compound |
order,structures |
R1130 |
T8372 |
T8371 |
punct |
-,order |
R1131 |
T8279 |
T8277 |
pobj |
heterochromatin,of |
R1132 |
T8373 |
T8368 |
conj |
structures,architecture |
R1133 |
T8280 |
T8272 |
punct |
", ",visualized |
R1134 |
T8375 |
T8366 |
acomp |
important,are |
R1135 |
T8376 |
T8375 |
prep |
for,important |
R1136 |
T8377 |
T8378 |
compound |
genome,function |
R1137 |
T8281 |
T8272 |
aux |
can,visualized |
R1138 |
T8378 |
T8376 |
pobj |
function,for |
R1139 |
T8379 |
T8380 |
punct |
[,21 |
R1140 |
T8282 |
T8272 |
advmod |
easily,visualized |
R1141 |
T8380 |
T8366 |
parataxis |
21,are |
R1142 |
T8381 |
T8380 |
punct |
],21 |
R1143 |
T8382 |
T8374 |
punct |
", ",decided |
R1144 |
T8383 |
T8374 |
nsubj |
we,decided |
R1145 |
T8384 |
T8385 |
aux |
to,characterize |
R1146 |
T8385 |
T8374 |
xcomp |
characterize,decided |
R1147 |
T8283 |
T8272 |
auxpass |
be,visualized |
R1148 |
T8386 |
T8387 |
det |
this,phenotype |
R1149 |
T8387 |
T8385 |
dobj |
phenotype,characterize |
R1150 |
T8388 |
T8385 |
prep |
in,characterize |
R1151 |
T8284 |
T8272 |
prep |
in,visualized |
R1152 |
T8389 |
T8390 |
amod |
more,detail |
R1153 |
T8390 |
T8388 |
pobj |
detail,in |
R1154 |
T8391 |
T8374 |
punct |
.,decided |
R1155 |
T8285 |
T8286 |
compound |
interphase,nuclei |
R1156 |
T8393 |
T8394 |
prep |
For,examined |
R1157 |
T8286 |
T8284 |
pobj |
nuclei,in |
R1158 |
T8395 |
T8396 |
det |
this,purpose |
R1159 |
T8396 |
T8393 |
pobj |
purpose,For |
R1160 |
T8397 |
T8394 |
punct |
", ",examined |
R1161 |
T8287 |
T8272 |
prep |
by,visualized |
R1162 |
T8398 |
T8394 |
nsubj |
we,examined |
R1163 |
T8399 |
T8400 |
det |
the,status |
R1164 |
T8400 |
T8394 |
dobj |
status,examined |
R1165 |
T8288 |
T8289 |
det |
the,DAPI |
R1166 |
T8401 |
T8400 |
prep |
of,status |
R1167 |
T8402 |
T8403 |
compound |
heterochromatin,organization |
R1168 |
T8403 |
T8401 |
pobj |
organization,of |
R1169 |
T8289 |
T8287 |
pobj |
DAPI,by |
R1170 |
T8404 |
T8394 |
prep |
between,examined |
R1171 |
T8405 |
T8406 |
det |
the,stages |
R1172 |
T8406 |
T8404 |
pobj |
stages,between |
R1173 |
T8407 |
T8408 |
nummod |
two,cell |
R1174 |
T8290 |
T8289 |
compound |
fluorochrome,DAPI |
R1175 |
T8408 |
T8406 |
nmod |
cell,stages |
R1176 |
T8409 |
T8408 |
punct |
-,cell |
R1177 |
T8410 |
T8408 |
cc |
and,cell |
R1178 |
T8291 |
T8287 |
cc |
and,by |
R1179 |
T8411 |
T8408 |
conj |
blastocyst,cell |
R1180 |
T8412 |
T8394 |
punct |
.,examined |
R1181 |
T8292 |
T8287 |
conj |
by,by |
R1182 |
T8414 |
T8415 |
prep |
In,is |
R1183 |
T8293 |
T8292 |
pcomp |
immunostaining,by |
R1184 |
T8416 |
T8417 |
nummod |
two,cell |
R1185 |
T8417 |
T8419 |
nmod |
cell,stage |
R1186 |
T8294 |
T8293 |
prep |
with,immunostaining |
R1187 |
T8418 |
T8417 |
punct |
-,cell |
R1188 |
T8419 |
T8420 |
nmod |
stage,embryos |
R1189 |
T8420 |
T8414 |
pobj |
embryos,In |
R1190 |
T8295 |
T8294 |
pobj |
HP1α,with |
R1191 |
T8421 |
T8422 |
amod |
wild,type |
R1192 |
T8422 |
T8420 |
compound |
type,embryos |
R1193 |
T8423 |
T8422 |
punct |
-,type |
R1194 |
T8296 |
T8297 |
punct |
[,16 |
R1195 |
T8424 |
T8415 |
punct |
", ",is |
R1196 |
T8425 |
T8426 |
compound |
DAPI,staining |
R1197 |
T8426 |
T8415 |
nsubj |
staining,is |
R1198 |
T8297 |
T8272 |
parataxis |
16,visualized |
R1199 |
T8427 |
T8415 |
acomp |
diffuse,is |
R1200 |
T8428 |
T8427 |
cc |
and,diffuse |
R1201 |
T8429 |
T8427 |
conj |
fibrillar,diffuse |
R1202 |
T8430 |
T8415 |
punct |
", ",is |
R1203 |
T8298 |
T8297 |
punct |
",",16 |
R1204 |
T8431 |
T8415 |
prep |
with,is |
R1205 |
T8432 |
T8431 |
pobj |
regions,with |
R1206 |
T8299 |
T8297 |
appos |
17,16 |
R1207 |
T8300 |
T8301 |
punct |
–,19 |
R1208 |
T8301 |
T8299 |
prep |
19,17 |
R1209 |
T8302 |
T8297 |
punct |
],16 |
R1210 |
T8433 |
T8432 |
prep |
of,regions |
R1211 |
T8303 |
T8272 |
punct |
.,visualized |
R1212 |
T8434 |
T8435 |
amod |
higher,density |
R1213 |
T8435 |
T8433 |
pobj |
density,of |
R1214 |
T8436 |
T8432 |
prep |
around,regions |
R1215 |
T8305 |
T8306 |
prep |
In,cluster |
R1216 |
T8437 |
T8438 |
det |
the,bodies |
R1217 |
T8438 |
T8436 |
pobj |
bodies,around |
R1218 |
T8439 |
T8440 |
amod |
nucleolar,precursor |
R1219 |
T8307 |
T8308 |
amod |
wild,type |
R1220 |
T8440 |
T8438 |
compound |
precursor,bodies |
R1221 |
T8441 |
T8442 |
punct |
(,1E |
R1222 |
T8442 |
T8415 |
parataxis |
1E,is |
R1223 |
T8443 |
T8442 |
compound |
Figure,1E |
R1224 |
T8444 |
T8442 |
punct |
),1E |
R1225 |
T8308 |
T8310 |
compound |
type,blastocysts |
R1226 |
T8445 |
T8415 |
punct |
.,is |
R1227 |
T8447 |
T8448 |
compound |
Heterochromatin,domains |
R1228 |
T8309 |
T8308 |
punct |
-,type |
R1229 |
T8448 |
T8449 |
nsubjpass |
domains,assembled |
R1230 |
T8310 |
T8305 |
pobj |
blastocysts,In |
R1231 |
T8450 |
T8449 |
auxpass |
are,assembled |
R1232 |
T8451 |
T8449 |
advmod |
progressively,assembled |
R1233 |
T8452 |
T8449 |
prep |
into,assembled |
R1234 |
T8311 |
T8305 |
cc |
and,In |
R1235 |
T8453 |
T8454 |
npadvmod |
DAPI,dense |
R1236 |
T8454 |
T8456 |
amod |
dense,foci |
R1237 |
T8455 |
T8454 |
punct |
-,dense |
R1238 |
T8456 |
T8452 |
pobj |
foci,into |
R1239 |
T8312 |
T8305 |
conj |
in,In |
R1240 |
T8457 |
T8449 |
prep |
between,assembled |
R1241 |
T8458 |
T8459 |
det |
the,stages |
R1242 |
T8313 |
T8314 |
nmod |
mouse,cells |
R1243 |
T8459 |
T8457 |
pobj |
stages,between |
R1244 |
T8460 |
T8461 |
nummod |
four,cell |
R1245 |
T8461 |
T8459 |
nmod |
cell,stages |
R1246 |
T8462 |
T8461 |
punct |
-,cell |
R1247 |
T8463 |
T8461 |
cc |
and,cell |
R1248 |
T8464 |
T8465 |
nummod |
32,cell |
R1249 |
T8314 |
T8312 |
pobj |
cells,in |
R1250 |
T8465 |
T8461 |
conj |
cell,cell |
R1251 |
T8466 |
T8465 |
punct |
-,cell |
R1252 |
T8467 |
T8459 |
compound |
blastocyst,stages |
R1253 |
T8468 |
T8469 |
punct |
(,1F |
R1254 |
T8315 |
T8314 |
amod |
somatic,cells |
R1255 |
T8469 |
T8449 |
parataxis |
1F,assembled |
R1256 |
T8470 |
T8469 |
compound |
Figure,1F |
R1257 |
T8471 |
T8469 |
punct |
),1F |
R1258 |
T8472 |
T8449 |
punct |
.,assembled |
R1259 |
T8316 |
T8306 |
punct |
", ",cluster |
R1260 |
T8474 |
T8475 |
nsubj |
Localization,confirmed |
R1261 |
T8317 |
T8318 |
amod |
pericentric,domains |
R1262 |
T8476 |
T8474 |
prep |
of,Localization |
R1263 |
T8477 |
T8476 |
pobj |
HP1α,of |
R1264 |
T8318 |
T8306 |
nsubj |
domains,cluster |
R1265 |
T8478 |
T8479 |
mark |
that,correspond |
R1266 |
T8479 |
T8475 |
ccomp |
correspond,confirmed |
R1267 |
T8480 |
T8481 |
det |
the,structures |
R1268 |
T8319 |
T8318 |
compound |
heterochromatin,domains |
R1269 |
T8481 |
T8479 |
nsubj |
structures,correspond |
R1270 |
T8482 |
T8481 |
acl |
visualized,structures |
R1271 |
T8483 |
T8482 |
prep |
by,visualized |
R1272 |
T8320 |
T8306 |
advmod |
together,cluster |
R1273 |
T8484 |
T8485 |
compound |
DAPI,staining |
R1274 |
T8485 |
T8483 |
pobj |
staining,by |
R1275 |
T8486 |
T8479 |
prep |
to,correspond |
R1276 |
T8321 |
T8306 |
cc |
and,cluster |
R1277 |
T8487 |
T8488 |
amod |
constitutive,heterochromatin |
R1278 |
T8488 |
T8486 |
pobj |
heterochromatin,to |
R1279 |
T8489 |
T8490 |
punct |
(,1E |
R1280 |
T8322 |
T8306 |
conj |
form,cluster |
R1281 |
T8490 |
T8475 |
parataxis |
1E,confirmed |
R1282 |
T8491 |
T8490 |
compound |
Figure,1E |
R1283 |
T8492 |
T8490 |
punct |
),1E |
R1284 |
T8323 |
T8324 |
amod |
higher,order |
R1285 |
T8493 |
T8475 |
punct |
.,confirmed |
R1286 |
T8495 |
T8496 |
advmod |
Thus,modified |
R1287 |
T8324 |
T8326 |
compound |
order,structures |
R1288 |
T8497 |
T8496 |
punct |
", ",modified |
R1289 |
T8498 |
T8499 |
det |
the,organization |
R1290 |
T8325 |
T8324 |
punct |
-,order |
R1291 |
T8499 |
T8496 |
nsubjpass |
organization,modified |
R1292 |
T8500 |
T8499 |
amod |
nuclear,organization |
R1293 |
T8501 |
T8499 |
prep |
of,organization |
R1294 |
T8326 |
T8322 |
dobj |
structures,form |
R1295 |
T8502 |
T8501 |
pobj |
heterochromatin,of |
R1296 |
T8503 |
T8496 |
auxpass |
is,modified |
R1297 |
T8504 |
T8496 |
advmod |
dramatically,modified |
R1298 |
T8505 |
T8496 |
prep |
during,modified |
R1299 |
T8506 |
T8507 |
amod |
pre-implantation,development |
R1300 |
T8507 |
T8505 |
pobj |
development,during |
R1301 |
T8327 |
T8326 |
compound |
chromatin,structures |
R1302 |
T8508 |
T8496 |
punct |
", ",modified |
R1303 |
T8509 |
T8496 |
prep |
between,modified |
R1304 |
T8510 |
T8511 |
det |
the,stages |
R1305 |
T8538 |
T8534 |
conj |
16,eight |
R1306 |
T8511 |
T8509 |
pobj |
stages,between |
R1307 |
T8512 |
T8513 |
nummod |
two,cell |
R1308 |
T8513 |
T8511 |
nmod |
cell,stages |
R1309 |
T8514 |
T8513 |
punct |
-,cell |
R1310 |
T8515 |
T8513 |
cc |
and,cell |
R1311 |
T8539 |
T8535 |
punct |
-,cell |
R1312 |
T8516 |
T8513 |
conj |
blastocyst,cell |
R1313 |
T8517 |
T8496 |
punct |
.,modified |
R1314 |
T8540 |
T8520 |
punct |
", ",were |
R1315 |
T8519 |
T8520 |
prep |
In,were |
R1316 |
T8521 |
T8522 |
nmod |
Chaf1a,embryos |
R1317 |
T8541 |
T8542 |
npadvmod |
DAPI,dense |
R1318 |
T8522 |
T8519 |
pobj |
embryos,In |
R1319 |
T8523 |
T8521 |
punct |
−,Chaf1a |
R1320 |
T8542 |
T8544 |
amod |
dense,foci |
R1321 |
T8524 |
T8521 |
punct |
/,Chaf1a |
R1322 |
T8525 |
T8521 |
punct |
−,Chaf1a |
R1323 |
T8526 |
T8522 |
compound |
E4,embryos |
R1324 |
T8543 |
T8542 |
punct |
-,dense |
R1325 |
T8527 |
T8522 |
punct |
", ",embryos |
R1326 |
T8528 |
T8529 |
dep |
which,arrested |
R1327 |
T8529 |
T8522 |
relcl |
arrested,embryos |
R1328 |
T8544 |
T8520 |
nsubj |
foci,were |
R1329 |
T8530 |
T8529 |
auxpass |
are,arrested |
R1330 |
T8531 |
T8529 |
prep |
between,arrested |
R1331 |
T8532 |
T8533 |
det |
the,stage |
R1332 |
T8545 |
T8546 |
advmod |
barely,detectable |
R1333 |
T8533 |
T8531 |
pobj |
stage,between |
R1334 |
T8534 |
T8535 |
nummod |
eight,cell |
R1335 |
T8535 |
T8533 |
compound |
cell,stage |
R1336 |
T8546 |
T8520 |
acomp |
detectable,were |
R1337 |
T8536 |
T8534 |
punct |
-,eight |
R1338 |
T8537 |
T8534 |
cc |
and,eight |
R1339 |
T8547 |
T8548 |
punct |
(,1D |
R1340 |
T8548 |
T8520 |
parataxis |
1D,were |
R1341 |
T8549 |
T8548 |
compound |
Figure,1D |
R1342 |
T8550 |
T8548 |
punct |
),1D |
R1343 |
T8643 |
T8642 |
punct |
-,type |
R1344 |
T8551 |
T8520 |
punct |
.,were |
R1345 |
T8644 |
T8645 |
punct |
(,compare |
R1346 |
T8645 |
T8617 |
parataxis |
compare,is |
R1347 |
T8553 |
T8554 |
advmod |
Instead,was |
R1348 |
T8646 |
T8647 |
compound |
Figure,1D |
R1349 |
T8647 |
T8645 |
dobj |
1D,compare |
R1350 |
T8648 |
T8647 |
cc |
and,1D |
R1351 |
T8649 |
T8647 |
conj |
1F,1D |
R1352 |
T8650 |
T8645 |
punct |
),compare |
R1353 |
T8651 |
T8617 |
punct |
.,is |
R1354 |
T8653 |
T8654 |
det |
These,data |
R1355 |
T8654 |
T8655 |
nsubj |
data,evidence |
R1356 |
T8656 |
T8657 |
det |
a,role |
R1357 |
T8657 |
T8655 |
dobj |
role,evidence |
R1358 |
T8555 |
T8554 |
punct |
", ",was |
R1359 |
T8658 |
T8657 |
amod |
key,role |
R1360 |
T8659 |
T8657 |
prep |
for,role |
R1361 |
T8660 |
T8659 |
pobj |
p150CAF,for |
R1362 |
T8661 |
T8660 |
punct |
-,p150CAF |
R1363 |
T8556 |
T8557 |
compound |
DAPI,staining |
R1364 |
T8662 |
T8660 |
nummod |
1,p150CAF |
R1365 |
T8663 |
T8655 |
prep |
during,evidence |
R1366 |
T8664 |
T8665 |
amod |
pre-implantation,development |
R1367 |
T8665 |
T8663 |
pobj |
development,during |
R1368 |
T8557 |
T8554 |
nsubj |
staining,was |
R1369 |
T8666 |
T8655 |
punct |
", ",evidence |
R1370 |
T8667 |
T8655 |
cc |
and,evidence |
R1371 |
T8668 |
T8655 |
conj |
reveal,evidence |
R1372 |
T8558 |
T8554 |
acomp |
diffuse,was |
R1373 |
T8669 |
T8670 |
mark |
that,required |
R1374 |
T8670 |
T8668 |
ccomp |
required,reveal |
R1375 |
T8671 |
T8672 |
det |
this,protein |
R1376 |
T8559 |
T8554 |
prep |
within,was |
R1377 |
T8672 |
T8670 |
nsubjpass |
protein,required |
R1378 |
T8673 |
T8670 |
auxpass |
is,required |
R1379 |
T8674 |
T8670 |
prep |
for,required |
R1380 |
T8675 |
T8676 |
det |
the,organization |
R1381 |
T8560 |
T8561 |
det |
the,nucleus |
R1382 |
T8676 |
T8674 |
pobj |
organization,for |
R1383 |
T8677 |
T8676 |
amod |
proper,organization |
R1384 |
T8678 |
T8679 |
nummod |
3,D |
R1385 |
T8561 |
T8559 |
pobj |
nucleus,within |
R1386 |
T8679 |
T8676 |
compound |
D,organization |
R1387 |
T8680 |
T8679 |
punct |
-,D |
R1388 |
T8681 |
T8676 |
prep |
of,organization |
R1389 |
T8562 |
T8554 |
punct |
", ",was |
R1390 |
T8682 |
T8681 |
pobj |
heterochromatin,of |
R1391 |
T8563 |
T8554 |
prep |
with,was |
R1392 |
T8683 |
T8670 |
prep |
within,required |
R1393 |
T8564 |
T8563 |
pobj |
regions,with |
R1394 |
T8684 |
T8685 |
amod |
embryonic,nuclei |
R1395 |
T8685 |
T8683 |
pobj |
nuclei,within |
R1396 |
T8686 |
T8685 |
compound |
cell,nuclei |
R1397 |
T8565 |
T8564 |
prep |
of,regions |
R1398 |
T8687 |
T8655 |
punct |
.,evidence |
R1399 |
T8689 |
T8690 |
advmod |
Next,wondered |
R1400 |
T8566 |
T8567 |
amod |
higher,density |
R1401 |
T8691 |
T8690 |
punct |
", ",wondered |
R1402 |
T8692 |
T8690 |
nsubj |
we,wondered |
R1403 |
T8693 |
T8694 |
mark |
whether,observed |
R1404 |
T8694 |
T8690 |
ccomp |
observed,wondered |
R1405 |
T8695 |
T8696 |
det |
a,requirement |
R1406 |
T8696 |
T8694 |
nsubjpass |
requirement,observed |
R1407 |
T8567 |
T8565 |
pobj |
density,of |
R1408 |
T8697 |
T8696 |
amod |
similar,requirement |
R1409 |
T8698 |
T8696 |
prep |
for,requirement |
R1410 |
T8568 |
T8567 |
prep |
around,density |
R1411 |
T8699 |
T8698 |
pobj |
CAF,for |
R1412 |
T8700 |
T8699 |
punct |
-,CAF |
R1413 |
T8701 |
T8699 |
nummod |
1,CAF |
R1414 |
T8702 |
T8694 |
aux |
could,observed |
R1415 |
T8569 |
T8570 |
det |
the,nucleoli |
R1416 |
T8703 |
T8694 |
advmod |
also,observed |
R1417 |
T8704 |
T8694 |
auxpass |
be,observed |
R1418 |
T8705 |
T8694 |
prep |
in,observed |
R1419 |
T8570 |
T8568 |
pobj |
nucleoli,around |
R1420 |
T8706 |
T8707 |
compound |
ES,cells |
R1421 |
T8707 |
T8705 |
pobj |
cells,in |
R1422 |
T8708 |
T8707 |
punct |
", ",cells |
R1423 |
T8571 |
T8568 |
cc |
and,around |
R1424 |
T8709 |
T8710 |
dep |
which,derived |
R1425 |
T8710 |
T8707 |
relcl |
derived,cells |
R1426 |
T8711 |
T8710 |
auxpass |
are,derived |
R1427 |
T8712 |
T8710 |
prep |
from,derived |
R1428 |
T8572 |
T8568 |
conj |
at,around |
R1429 |
T8713 |
T8714 |
det |
the,mass |
R1430 |
T8714 |
T8712 |
pobj |
mass,from |
R1431 |
T8715 |
T8714 |
nmod |
blastocyst,mass |
R1432 |
T8716 |
T8717 |
amod |
inner,cell |
R1433 |
T8573 |
T8574 |
det |
the,periphery |
R1434 |
T8717 |
T8714 |
compound |
cell,mass |
R1435 |
T8718 |
T8690 |
punct |
.,wondered |
R1436 |
T8574 |
T8572 |
pobj |
periphery,at |
R1437 |
T8720 |
T8721 |
prep |
Given,derived |
R1438 |
T8722 |
T8723 |
det |
the,arrest |
R1439 |
T8575 |
T8574 |
prep |
of,periphery |
R1440 |
T8723 |
T8720 |
pobj |
arrest,Given |
R1441 |
T8724 |
T8723 |
amod |
early,arrest |
R1442 |
T8725 |
T8723 |
amod |
developmental,arrest |
R1443 |
T8576 |
T8577 |
det |
the,nuclei |
R1444 |
T8726 |
T8723 |
acl |
observed,arrest |
R1445 |
T8727 |
T8726 |
prep |
in,observed |
R1446 |
T8728 |
T8729 |
nmod |
Chaf1a,embryos |
R1447 |
T8577 |
T8575 |
pobj |
nuclei,of |
R1448 |
T8729 |
T8727 |
pobj |
embryos,in |
R1449 |
T8730 |
T8728 |
punct |
−,Chaf1a |
R1450 |
T8731 |
T8728 |
punct |
/,Chaf1a |
R1451 |
T8578 |
T8579 |
punct |
(,1D |
R1452 |
T8732 |
T8728 |
punct |
−,Chaf1a |
R1453 |
T8733 |
T8721 |
punct |
", ",derived |
R1454 |
T8734 |
T8735 |
amod |
such,cells |
R1455 |
T8735 |
T8721 |
nsubjpass |
cells,derived |
R1456 |
T8736 |
T8721 |
aux |
could,derived |
R1457 |
T8737 |
T8721 |
neg |
not,derived |
R1458 |
T8738 |
T8721 |
auxpass |
be,derived |
R1459 |
T8579 |
T8554 |
parataxis |
1D,was |
R1460 |
T8739 |
T8721 |
advmod |
directly,derived |
R1461 |
T8740 |
T8721 |
prep |
from,derived |
R1462 |
T8741 |
T8742 |
amod |
null,embryos |
R1463 |
T8580 |
T8579 |
compound |
Figure,1D |
R1464 |
T8742 |
T8740 |
pobj |
embryos,from |
R1465 |
T8743 |
T8721 |
punct |
", ",derived |
R1466 |
T8744 |
T8721 |
cc |
and,derived |
R1467 |
T8581 |
T8579 |
punct |
),1D |
R1468 |
T8745 |
T8746 |
nsubj |
we,used |
R1469 |
T8746 |
T8721 |
conj |
used,derived |
R1470 |
T8747 |
T8746 |
advmod |
thus,used |
R1471 |
T8748 |
T8749 |
det |
an,strategy |
R1472 |
T8582 |
T8554 |
punct |
.,was |
R1473 |
T8584 |
T8585 |
nsubj |
Localization,showed |
R1474 |
T8586 |
T8584 |
prep |
of,Localization |
R1475 |
T8749 |
T8746 |
dobj |
strategy,used |
R1476 |
T8750 |
T8749 |
compound |
RNAi,strategy |
R1477 |
T8751 |
T8752 |
punct |
(,2A |
R1478 |
T8587 |
T8586 |
pobj |
HP1α,of |
R1479 |
T8752 |
T8746 |
parataxis |
2A,used |
R1480 |
T8753 |
T8752 |
compound |
Figure,2A |
R1481 |
T8754 |
T8752 |
punct |
),2A |
R1482 |
T8755 |
T8721 |
punct |
.,derived |
R1483 |
T8588 |
T8589 |
det |
a,pattern |
R1484 |
T8757 |
T8758 |
nmod |
p150CAF,knockdown |
R1485 |
T8758 |
T8761 |
nsubjpass |
knockdown,quantified |
R1486 |
T8589 |
T8585 |
dobj |
pattern,showed |
R1487 |
T8759 |
T8757 |
punct |
-,p150CAF |
R1488 |
T8760 |
T8757 |
nummod |
1,p150CAF |
R1489 |
T8590 |
T8589 |
amod |
diffuse,pattern |
R1490 |
T8762 |
T8758 |
prep |
in,knockdown |
R1491 |
T8763 |
T8764 |
compound |
ES,cells |
R1492 |
T8764 |
T8762 |
pobj |
cells,in |
R1493 |
T8591 |
T8589 |
amod |
similar,pattern |
R1494 |
T8765 |
T8761 |
auxpass |
was,quantified |
R1495 |
T8766 |
T8761 |
prep |
by,quantified |
R1496 |
T8592 |
T8591 |
prep |
to,similar |
R1497 |
T8767 |
T8768 |
compound |
Western,blot |
R1498 |
T8768 |
T8769 |
compound |
blot,analysis |
R1499 |
T8769 |
T8766 |
pobj |
analysis,by |
R1500 |
T8593 |
T8594 |
det |
the,DAPI |
R1501 |
T8594 |
T8592 |
pobj |
DAPI,to |
R1502 |
T8770 |
T8769 |
cc |
and,analysis |
R1503 |
T8595 |
T8589 |
punct |
", ",pattern |
R1504 |
T8771 |
T8769 |
conj |
IF,analysis |
R1505 |
T8772 |
T8761 |
punct |
.,quantified |
R1506 |
T8774 |
T8775 |
punct |
(,S1 |
R1507 |
T8776 |
T8775 |
compound |
Figure,S1 |
R1508 |
T8777 |
T8775 |
punct |
),S1 |
R1509 |
T8596 |
T8589 |
prep |
with,pattern |
R1510 |
T8778 |
T8775 |
punct |
.,S1 |
R1511 |
T8780 |
T8781 |
nmod |
p150CAF,cells |
R1512 |
T8597 |
T8598 |
det |
some,enrichment |
R1513 |
T8781 |
T8788 |
nsubj |
cells,displayed |
R1514 |
T8782 |
T8780 |
punct |
-,p150CAF |
R1515 |
T8598 |
T8596 |
pobj |
enrichment,with |
R1516 |
T8783 |
T8780 |
nummod |
1,p150CAF |
R1517 |
T8784 |
T8785 |
npadvmod |
RNAi,depleted |
R1518 |
T8785 |
T8781 |
amod |
depleted,cells |
R1519 |
T8599 |
T8598 |
prep |
at,enrichment |
R1520 |
T8786 |
T8785 |
punct |
-,depleted |
R1521 |
T8787 |
T8781 |
compound |
ES,cells |
R1522 |
T8600 |
T8601 |
det |
the,periphery |
R1523 |
T8789 |
T8790 |
det |
a,phenotype |
R1524 |
T8790 |
T8788 |
dobj |
phenotype,displayed |
R1525 |
T8791 |
T8792 |
advmod |
very,similar |
R1526 |
T8792 |
T8790 |
amod |
similar,phenotype |
R1527 |
T8601 |
T8599 |
pobj |
periphery,at |
R1528 |
T8793 |
T8792 |
prep |
to,similar |
R1529 |
T8794 |
T8795 |
det |
the,cells |
R1530 |
T8795 |
T8793 |
pobj |
cells,to |
R1531 |
T8602 |
T8601 |
amod |
nuclear,periphery |
R1532 |
T8796 |
T8795 |
prep |
of,cells |
R1533 |
T8797 |
T8798 |
nmod |
Chaf1a,mice |
R1534 |
T8798 |
T8796 |
pobj |
mice,of |
R1535 |
T8799 |
T8797 |
punct |
−,Chaf1a |
R1536 |
T8603 |
T8599 |
punct |
", ",at |
R1537 |
T8800 |
T8797 |
punct |
/,Chaf1a |
R1538 |
T8801 |
T8797 |
punct |
−,Chaf1a |
R1539 |
T8802 |
T8798 |
compound |
E4,mice |
R1540 |
T8604 |
T8599 |
cc |
and,at |
R1541 |
T8803 |
T8788 |
punct |
.,displayed |
R1542 |
T8805 |
T8806 |
npadvmod |
DAPI,dense |
R1543 |
T8605 |
T8599 |
conj |
around,at |
R1544 |
T8806 |
T8808 |
amod |
dense,foci |
R1545 |
T8807 |
T8806 |
punct |
-,dense |
R1546 |
T8808 |
T8809 |
nsubjpass |
foci,lost |
R1547 |
T8606 |
T8607 |
det |
the,nucleoli |
R1548 |
T8810 |
T8809 |
auxpass |
were,lost |
R1549 |
T8811 |
T8809 |
punct |
", ",lost |
R1550 |
T8812 |
T8809 |
cc |
and,lost |
R1551 |
T8607 |
T8605 |
pobj |
nucleoli,around |
R1552 |
T8813 |
T8814 |
nsubj |
we,observed |
R1553 |
T8814 |
T8809 |
conj |
observed,lost |
R1554 |
T8815 |
T8816 |
amod |
diffuse,staining |
R1555 |
T8608 |
T8609 |
punct |
(,1D |
R1556 |
T8816 |
T8814 |
dobj |
staining,observed |
R1557 |
T8817 |
T8816 |
nmod |
HP1α,staining |
R1558 |
T8818 |
T8817 |
cc |
and,HP1α |
R1559 |
T8819 |
T8817 |
conj |
DAPI,HP1α |
R1560 |
T8820 |
T8814 |
prep |
around,observed |
R1561 |
T8821 |
T8822 |
det |
the,nucleoli |
R1562 |
T8822 |
T8820 |
pobj |
nucleoli,around |
R1563 |
T8609 |
T8585 |
parataxis |
1D,showed |
R1564 |
T8823 |
T8820 |
cc |
and,around |
R1565 |
T8824 |
T8820 |
conj |
at,around |
R1566 |
T8610 |
T8609 |
compound |
Figure,1D |
R1567 |
T8825 |
T8826 |
det |
the,periphery |
R1568 |
T8826 |
T8824 |
pobj |
periphery,at |
R1569 |
T8827 |
T8826 |
prep |
of,periphery |
R1570 |
T8828 |
T8829 |
det |
the,nucleus |
R1571 |
T8829 |
T8827 |
pobj |
nucleus,of |
R1572 |
T8611 |
T8609 |
punct |
),1D |
R1573 |
T8830 |
T8831 |
punct |
(,2B |
R1574 |
T8831 |
T8814 |
parataxis |
2B,observed |
R1575 |
T8832 |
T8831 |
compound |
Figure,2B |
R1576 |
T8612 |
T8585 |
punct |
.,showed |
R1577 |
T8833 |
T8831 |
punct |
),2B |
R1578 |
T8834 |
T8814 |
punct |
.,observed |
R1579 |
T8614 |
T8615 |
det |
This,organization |
R1580 |
T8836 |
T8837 |
det |
This,result |
R1581 |
T8837 |
T8838 |
nsubj |
result,indicates |
R1582 |
T8839 |
T8840 |
mark |
that,is |
R1583 |
T8615 |
T8617 |
nsubj |
organization,is |
R1584 |
T8840 |
T8838 |
ccomp |
is,indicates |
R1585 |
T8841 |
T8840 |
nsubj |
p150CAF,is |
R1586 |
T8842 |
T8841 |
punct |
-,p150CAF |
R1587 |
T8616 |
T8615 |
amod |
abnormal,organization |
R1588 |
T8843 |
T8841 |
nummod |
1,p150CAF |
R1589 |
T8844 |
T8840 |
acomp |
essential,is |
R1590 |
T8845 |
T8844 |
prep |
for,essential |
R1591 |
T8618 |
T8615 |
prep |
of,organization |
R1592 |
T8846 |
T8847 |
amod |
nuclear,organization |
R1593 |
T8847 |
T8845 |
pobj |
organization,for |
R1594 |
T8848 |
T8847 |
prep |
of,organization |
R1595 |
T8849 |
T8848 |
pobj |
heterochromatin,of |
R1596 |
T8619 |
T8618 |
pobj |
heterochromatin,of |
R1597 |
T8850 |
T8849 |
prep |
in,heterochromatin |
R1598 |
T8851 |
T8852 |
compound |
ES,cells |
R1599 |
T8852 |
T8850 |
pobj |
cells,in |
R1600 |
T8620 |
T8615 |
prep |
in,organization |
R1601 |
T8853 |
T8847 |
prep |
in,organization |
R1602 |
T8621 |
T8622 |
nmod |
Chaf1a,embryos |
R1603 |
T8622 |
T8620 |
pobj |
embryos,in |
R1604 |
T8854 |
T8855 |
det |
a,way |
R1605 |
T8623 |
T8621 |
punct |
−,Chaf1a |
R1606 |
T8855 |
T8853 |
pobj |
way,in |
R1607 |
T8856 |
T8855 |
amod |
similar,way |
R1608 |
T8857 |
T8855 |
prep |
to,way |
R1609 |
T8624 |
T8621 |
punct |
/,Chaf1a |
R1610 |
T8858 |
T8859 |
amod |
early,embryos |
R1611 |
T8859 |
T8857 |
pobj |
embryos,to |
R1612 |
T8860 |
T8859 |
amod |
pre-implantation,embryos |
R1613 |
T8861 |
T8838 |
punct |
.,indicates |
R1614 |
T8625 |
T8621 |
punct |
−,Chaf1a |
R1615 |
T8863 |
T8864 |
advmod |
Surprisingly,observe |
R1616 |
T8626 |
T8617 |
acomp |
reminiscent,is |
R1617 |
T8865 |
T8864 |
punct |
", ",observe |
R1618 |
T8866 |
T8864 |
nsubj |
we,observe |
R1619 |
T8867 |
T8864 |
aux |
did,observe |
R1620 |
T8868 |
T8864 |
neg |
not,observe |
R1621 |
T8627 |
T8626 |
prep |
of,reminiscent |
R1622 |
T8869 |
T8870 |
det |
this,alteration |
R1623 |
T8628 |
T8629 |
det |
the,organization |
R1624 |
T8870 |
T8864 |
dobj |
alteration,observe |
R1625 |
T8871 |
T8870 |
amod |
severe,alteration |
R1626 |
T8872 |
T8870 |
prep |
in,alteration |
R1627 |
T8873 |
T8874 |
compound |
heterochromatin,organization |
R1628 |
T8629 |
T8627 |
pobj |
organization,of |
R1629 |
T8874 |
T8872 |
pobj |
organization,in |
R1630 |
T8875 |
T8864 |
prep |
in,observe |
R1631 |
T8876 |
T8877 |
amod |
primary,fibroblasts |
R1632 |
T8630 |
T8629 |
compound |
heterochromatin,organization |
R1633 |
T8877 |
T8875 |
pobj |
fibroblasts,in |
R1634 |
T8878 |
T8877 |
nmod |
mouse,fibroblasts |
R1635 |
T8879 |
T8877 |
amod |
embryonic,fibroblasts |
R1636 |
T8880 |
T8877 |
punct |
(,fibroblasts |
R1637 |
T8631 |
T8629 |
acl |
found,organization |
R1638 |
T8881 |
T8877 |
appos |
MEFs,fibroblasts |
R1639 |
T8882 |
T8864 |
punct |
),observe |
R1640 |
T8883 |
T8864 |
prep |
following,observe |
R1641 |
T8632 |
T8631 |
prep |
in,found |
R1642 |
T8884 |
T8885 |
nmod |
p150CAF,depletion |
R1643 |
T8885 |
T8883 |
pobj |
depletion,following |
R1644 |
T8886 |
T8884 |
punct |
-,p150CAF |
R1645 |
T8633 |
T8634 |
quantmod |
two,four |
R1646 |
T8887 |
T8884 |
nummod |
1,p150CAF |
R1647 |
T8888 |
T8885 |
prep |
by,depletion |
R1648 |
T8889 |
T8888 |
pobj |
RNAi,by |
R1649 |
T8634 |
T8637 |
nummod |
four,cell |
R1650 |
T8890 |
T8864 |
punct |
.,observe |
R1651 |
T8892 |
T8893 |
nmod |
p150CAF,depletion |
R1652 |
T8635 |
T8634 |
punct |
-,four |
R1653 |
T8893 |
T8896 |
nsubj |
depletion,resulted |
R1654 |
T8894 |
T8892 |
punct |
-,p150CAF |
R1655 |
T8895 |
T8892 |
nummod |
1,p150CAF |
R1656 |
T8636 |
T8634 |
quantmod |
to,four |
R1657 |
T8897 |
T8893 |
prep |
in,depletion |
R1658 |
T8898 |
T8897 |
pobj |
MEFs,in |
R1659 |
T8637 |
T8639 |
nmod |
cell,stage |
R1660 |
T8899 |
T8896 |
prep |
in,resulted |
R1661 |
T8900 |
T8901 |
det |
a,inhibition |
R1662 |
T8901 |
T8899 |
pobj |
inhibition,in |
R1663 |
T8902 |
T8901 |
amod |
strong,inhibition |
R1664 |
T8903 |
T8901 |
prep |
of,inhibition |
R1665 |
T8904 |
T8905 |
compound |
cell,proliferation |
R1666 |
T8905 |
T8903 |
pobj |
proliferation,of |
R1667 |
T8638 |
T8637 |
punct |
-,cell |
R1668 |
T8906 |
T8907 |
punct |
(,data |
R1669 |
T8907 |
T8896 |
meta |
data,resulted |
R1670 |
T8908 |
T8907 |
amod |
unpublished,data |
R1671 |
T8909 |
T8907 |
punct |
),data |
R1672 |
T8639 |
T8640 |
nmod |
stage,embryos |
R1673 |
T8910 |
T8896 |
punct |
", ",resulted |
R1674 |
T8911 |
T8896 |
cc |
but,resulted |
R1675 |
T8912 |
T8913 |
aux |
did,alter |
R1676 |
T8913 |
T8896 |
conj |
alter,resulted |
R1677 |
T8640 |
T8632 |
pobj |
embryos,in |
R1678 |
T8914 |
T8913 |
neg |
not,alter |
R1679 |
T8915 |
T8916 |
det |
the,clustering |
R1680 |
T8916 |
T8913 |
dobj |
clustering,alter |
R1681 |
T8641 |
T8642 |
amod |
wild,type |
R1682 |
T8917 |
T8916 |
prep |
of,clustering |
R1683 |
T8918 |
T8919 |
compound |
heterochromatin,domains |
R1684 |
T8919 |
T8917 |
pobj |
domains,of |
R1685 |
T8642 |
T8640 |
compound |
type,embryos |
R1686 |
T8920 |
T8921 |
punct |
(,2C |
R1687 |
T8921 |
T8913 |
parataxis |
2C,alter |
R1688 |
T8922 |
T8921 |
compound |
Figures,2C |
R1689 |
T8959 |
T8957 |
pobj |
embryo,in |
R1690 |
T8923 |
T8921 |
cc |
and,2C |
R1691 |
T8924 |
T8921 |
conj |
S2,2C |
R1692 |
T8925 |
T8921 |
punct |
),2C |
R1693 |
T8926 |
T8896 |
punct |
.,resulted |
R1694 |
T8928 |
T8929 |
advmod |
Similarly,affect |
R1695 |
T8960 |
T8959 |
amod |
early,embryo |
R1696 |
T8930 |
T8929 |
punct |
", ",affect |
R1697 |
T8931 |
T8932 |
nmod |
p150CAF,depletion |
R1698 |
T8961 |
T8959 |
cc |
and,embryo |
R1699 |
T8932 |
T8929 |
nsubj |
depletion,affect |
R1700 |
T8933 |
T8931 |
punct |
-,p150CAF |
R1701 |
T8962 |
T8963 |
compound |
ES,cells |
R1702 |
T8934 |
T8931 |
nummod |
1,p150CAF |
R1703 |
T8935 |
T8932 |
prep |
in,depletion |
R1704 |
T8936 |
T8937 |
compound |
3T3,cells |
R1705 |
T8937 |
T8935 |
pobj |
cells,in |
R1706 |
T8963 |
T8959 |
conj |
cells,embryo |
R1707 |
T8938 |
T8929 |
aux |
did,affect |
R1708 |
T8939 |
T8929 |
neg |
not,affect |
R1709 |
T8940 |
T8941 |
compound |
heterochromatin,organization |
R1710 |
T8964 |
T8949 |
punct |
.,reveal |
R1711 |
T8941 |
T8929 |
dobj |
organization,affect |
R1712 |
T8942 |
T8943 |
punct |
[,16 |
R1713 |
T8943 |
T8929 |
parataxis |
16,affect |
R1714 |
T8944 |
T8943 |
punct |
],16 |
R1715 |
T8945 |
T8929 |
punct |
.,affect |
R1716 |
T8966 |
T8967 |
nsubj |
We,wondered |
R1717 |
T8947 |
T8948 |
det |
These,observations |
R1718 |
T8948 |
T8949 |
nsubj |
observations,reveal |
R1719 |
T8950 |
T8951 |
det |
a,function |
R1720 |
T8968 |
T8967 |
advmod |
also,wondered |
R1721 |
T8951 |
T8949 |
dobj |
function,reveal |
R1722 |
T8952 |
T8951 |
amod |
specific,function |
R1723 |
T8953 |
T8951 |
prep |
for,function |
R1724 |
T8954 |
T8953 |
pobj |
CAF,for |
R1725 |
T8955 |
T8954 |
punct |
-,CAF |
R1726 |
T8956 |
T8954 |
nummod |
1,CAF |
R1727 |
T8969 |
T8970 |
mark |
whether,affects |
R1728 |
T8957 |
T8951 |
prep |
in,function |
R1729 |
T8958 |
T8959 |
det |
the,embryo |
R1730 |
T8970 |
T8967 |
advcl |
affects,wondered |
R1731 |
T8971 |
T8970 |
nsubj |
loss,affects |
R1732 |
T8972 |
T8971 |
prep |
of,loss |
R1733 |
T8973 |
T8972 |
pobj |
p150CAF,of |
R1734 |
T9065 |
T9064 |
compound |
Figure,2D |
R1735 |
T8974 |
T8973 |
punct |
-,p150CAF |
R1736 |
T9066 |
T9064 |
punct |
),2D |
R1737 |
T9067 |
T9038 |
punct |
.,detect |
R1738 |
T8975 |
T8973 |
nummod |
1,p150CAF |
R1739 |
T9069 |
T9070 |
det |
These,data |
R1740 |
T9070 |
T9071 |
nsubj |
data,show |
R1741 |
T8976 |
T8971 |
prep |
in,loss |
R1742 |
T9072 |
T9073 |
mark |
that,altered |
R1743 |
T9073 |
T9071 |
ccomp |
altered,show |
R1744 |
T8977 |
T8978 |
compound |
ES,cells |
R1745 |
T9074 |
T9075 |
amod |
nuclear,architecture |
R1746 |
T9075 |
T9073 |
nsubjpass |
architecture,altered |
R1747 |
T9076 |
T9073 |
auxpass |
is,altered |
R1748 |
T8978 |
T8976 |
pobj |
cells,in |
R1749 |
T9077 |
T9073 |
neg |
not,altered |
R1750 |
T9078 |
T9073 |
advmod |
globally,altered |
R1751 |
T8979 |
T8970 |
advmod |
specifically,affects |
R1752 |
T9079 |
T9073 |
prep |
following,altered |
R1753 |
T9080 |
T9079 |
pobj |
CAF,following |
R1754 |
T9081 |
T9080 |
punct |
-,CAF |
R1755 |
T8980 |
T8981 |
det |
the,subcompartment |
R1756 |
T9082 |
T9080 |
nummod |
1,CAF |
R1757 |
T9083 |
T9080 |
appos |
loss,CAF |
R1758 |
T9084 |
T9083 |
punct |
-,loss |
R1759 |
T8981 |
T8970 |
dobj |
subcompartment,affects |
R1760 |
T9085 |
T9083 |
prep |
of,loss |
R1761 |
T9086 |
T9085 |
punct |
-,of |
R1762 |
T9087 |
T9085 |
pobj |
function,of |
R1763 |
T8982 |
T8981 |
nmod |
heterochromatin,subcompartment |
R1764 |
T9088 |
T9071 |
punct |
.,show |
R1765 |
T9090 |
T9091 |
prep |
In,revealed |
R1766 |
T9092 |
T9090 |
pobj |
addition,In |
R1767 |
T9093 |
T9091 |
punct |
", ",revealed |
R1768 |
T9094 |
T9095 |
compound |
Western,blot |
R1769 |
T8983 |
T8981 |
amod |
nuclear,subcompartment |
R1770 |
T9095 |
T9096 |
compound |
blot,analysis |
R1771 |
T9096 |
T9091 |
nsubj |
analysis,revealed |
R1772 |
T9097 |
T9098 |
mark |
that,result |
R1773 |
T8984 |
T8970 |
punct |
", ",affects |
R1774 |
T9098 |
T9091 |
ccomp |
result,revealed |
R1775 |
T9099 |
T9100 |
nmod |
CAF,depletion |
R1776 |
T9100 |
T9098 |
nsubj |
depletion,result |
R1777 |
T8985 |
T8970 |
cc |
or,affects |
R1778 |
T9101 |
T9099 |
punct |
-,CAF |
R1779 |
T9102 |
T9099 |
nummod |
1,CAF |
R1780 |
T9103 |
T9098 |
aux |
did,result |
R1781 |
T8986 |
T8987 |
mark |
whether,result |
R1782 |
T9104 |
T9098 |
neg |
not,result |
R1783 |
T9105 |
T9098 |
prep |
in,result |
R1784 |
T9106 |
T9107 |
amod |
altered,levels |
R1785 |
T8987 |
T8970 |
conj |
result,affects |
R1786 |
T9107 |
T9105 |
pobj |
levels,in |
R1787 |
T9108 |
T9107 |
prep |
of,levels |
R1788 |
T9109 |
T9110 |
nmod |
chromatin,proteins |
R1789 |
T8988 |
T8987 |
nsubj |
it,result |
R1790 |
T9110 |
T9108 |
pobj |
proteins,of |
R1791 |
T9111 |
T9110 |
amod |
architectural,proteins |
R1792 |
T9112 |
T9113 |
amod |
such,as |
R1793 |
T9113 |
T9110 |
prep |
as,proteins |
R1794 |
T8989 |
T8987 |
aux |
might,result |
R1795 |
T9114 |
T9115 |
compound |
histone,H3 |
R1796 |
T9115 |
T9113 |
pobj |
H3,as |
R1797 |
T9116 |
T9115 |
cc |
and,H3 |
R1798 |
T8990 |
T8987 |
prep |
in,result |
R1799 |
T9117 |
T9115 |
conj |
HP1α,H3 |
R1800 |
T9118 |
T9119 |
punct |
(,S1 |
R1801 |
T8991 |
T8992 |
det |
a,loss |
R1802 |
T9119 |
T9091 |
parataxis |
S1,revealed |
R1803 |
T8992 |
T8990 |
pobj |
loss,in |
R1804 |
T9120 |
T9119 |
compound |
Figure,S1 |
R1805 |
T9121 |
T9119 |
punct |
),S1 |
R1806 |
T9122 |
T9091 |
punct |
.,revealed |
R1807 |
T8993 |
T8994 |
advmod |
more,global |
R1808 |
T9124 |
T9125 |
advmod |
Thus,required |
R1809 |
T8994 |
T8992 |
amod |
global,loss |
R1810 |
T9126 |
T9125 |
punct |
", ",required |
R1811 |
T9127 |
T9125 |
nsubjpass |
CAF,required |
R1812 |
T8995 |
T8992 |
prep |
of,loss |
R1813 |
T9128 |
T9127 |
punct |
-,CAF |
R1814 |
T9129 |
T9127 |
nummod |
1,CAF |
R1815 |
T9130 |
T9125 |
auxpass |
is,required |
R1816 |
T8996 |
T8997 |
amod |
nuclear,organization |
R1817 |
T9131 |
T9125 |
advmod |
specifically,required |
R1818 |
T9132 |
T9125 |
prep |
in,required |
R1819 |
T9133 |
T9134 |
amod |
early,embryos |
R1820 |
T9134 |
T9132 |
pobj |
embryos,in |
R1821 |
T9135 |
T9132 |
cc |
and,in |
R1822 |
T9136 |
T9132 |
conj |
in,in |
R1823 |
T8997 |
T8995 |
pobj |
organization,of |
R1824 |
T9137 |
T9138 |
compound |
ES,cells |
R1825 |
T9138 |
T9136 |
pobj |
cells,in |
R1826 |
T9139 |
T9125 |
prep |
for,required |
R1827 |
T8998 |
T8997 |
cc |
and,organization |
R1828 |
T9140 |
T9141 |
det |
the,organization |
R1829 |
T9141 |
T9139 |
pobj |
organization,for |
R1830 |
T9142 |
T9141 |
amod |
proper,organization |
R1831 |
T8999 |
T8997 |
conj |
architecture,organization |
R1832 |
T9143 |
T9141 |
prep |
of,organization |
R1833 |
T9144 |
T9145 |
det |
the,compartment |
R1834 |
T9145 |
T9143 |
pobj |
compartment,of |
R1835 |
T9000 |
T8967 |
punct |
.,wondered |
R1836 |
T9146 |
T9145 |
nmod |
heterochromatin,compartment |
R1837 |
T9147 |
T9145 |
amod |
subnuclear,compartment |
R1838 |
T9148 |
T9125 |
punct |
.,required |
R1839 |
T9002 |
T9003 |
nsubj |
We,used |
R1840 |
T9150 |
T9151 |
amod |
Previous,studies |
R1841 |
T9151 |
T9152 |
nsubj |
studies,showed |
R1842 |
T9004 |
T9005 |
det |
a,antibody |
R1843 |
T9153 |
T9151 |
acl |
performed,studies |
R1844 |
T9154 |
T9153 |
prep |
in,performed |
R1845 |
T9155 |
T9156 |
amod |
mammalian,lines |
R1846 |
T9156 |
T9154 |
pobj |
lines,in |
R1847 |
T9157 |
T9156 |
compound |
cell,lines |
R1848 |
T9005 |
T9003 |
dobj |
antibody,used |
R1849 |
T9158 |
T9159 |
mark |
that,required |
R1850 |
T9159 |
T9152 |
ccomp |
required,showed |
R1851 |
T9160 |
T9161 |
nmod |
CAF,activity |
R1852 |
T9161 |
T9159 |
nsubjpass |
activity,required |
R1853 |
T9006 |
T9005 |
amod |
specific,antibody |
R1854 |
T9162 |
T9160 |
punct |
-,CAF |
R1855 |
T9163 |
T9160 |
nummod |
1,CAF |
R1856 |
T9164 |
T9159 |
auxpass |
is,required |
R1857 |
T9007 |
T9005 |
prep |
against,antibody |
R1858 |
T9165 |
T9159 |
prep |
for,required |
R1859 |
T9166 |
T9167 |
compound |
S,phase |
R1860 |
T9008 |
T9009 |
det |
the,protein |
R1861 |
T9167 |
T9168 |
compound |
phase,progression |
R1862 |
T9168 |
T9165 |
pobj |
progression,for |
R1863 |
T9169 |
T9170 |
punct |
[,24 |
R1864 |
T9009 |
T9007 |
pobj |
protein,against |
R1865 |
T9170 |
T9152 |
parataxis |
24,showed |
R1866 |
T9010 |
T9011 |
amod |
promyelocytic,leukemia |
R1867 |
T9011 |
T9009 |
nmod |
leukemia,protein |
R1868 |
T9171 |
T9170 |
nummod |
16,24 |
R1869 |
T9172 |
T9170 |
punct |
",",24 |
R1870 |
T9173 |
T9170 |
nummod |
23,24 |
R1871 |
T9174 |
T9170 |
punct |
",",24 |
R1872 |
T9175 |
T9170 |
punct |
],24 |
R1873 |
T9012 |
T9011 |
punct |
(,leukemia |
R1874 |
T9176 |
T9152 |
punct |
.,showed |
R1875 |
T9178 |
T9179 |
nsubj |
We,show |
R1876 |
T9013 |
T9011 |
appos |
PML,leukemia |
R1877 |
T9180 |
T9179 |
advmod |
here,show |
R1878 |
T9181 |
T9182 |
mark |
that,appear |
R1879 |
T9014 |
T9009 |
punct |
),protein |
R1880 |
T9015 |
T9016 |
aux |
to,study |
R1881 |
T9182 |
T9179 |
ccomp |
appear,show |
R1882 |
T9183 |
T9184 |
nummod |
3,d |
R1883 |
T9184 |
T9185 |
npadvmod |
d,after |
R1884 |
T9016 |
T9003 |
advcl |
study,used |
R1885 |
T9185 |
T9182 |
prep |
after,appear |
R1886 |
T9186 |
T9185 |
pobj |
transfection,after |
R1887 |
T9017 |
T9018 |
det |
the,fate |
R1888 |
T9187 |
T9186 |
prep |
of,transfection |
R1889 |
T9188 |
T9189 |
det |
the,vector |
R1890 |
T9189 |
T9187 |
pobj |
vector,of |
R1891 |
T9018 |
T9016 |
dobj |
fate,study |
R1892 |
T9190 |
T9189 |
compound |
RNAi,vector |
R1893 |
T9191 |
T9182 |
punct |
", ",appear |
R1894 |
T9192 |
T9193 |
npadvmod |
p150CAF,depleted |
R1895 |
T9019 |
T9018 |
prep |
of,fate |
R1896 |
T9193 |
T9197 |
amod |
depleted,cells |
R1897 |
T9194 |
T9192 |
punct |
-,p150CAF |
R1898 |
T9195 |
T9192 |
nummod |
1,p150CAF |
R1899 |
T9020 |
T9021 |
det |
the,bodies |
R1900 |
T9196 |
T9193 |
punct |
-,depleted |
R1901 |
T9197 |
T9182 |
nsubj |
cells,appear |
R1902 |
T9198 |
T9197 |
punct |
(,cells |
R1903 |
T9021 |
T9019 |
pobj |
bodies,of |
R1904 |
T9199 |
T9197 |
acl |
identified,cells |
R1905 |
T9200 |
T9199 |
agent |
by,identified |
R1906 |
T9201 |
T9202 |
det |
a,absence |
R1907 |
T9022 |
T9021 |
nmod |
PML,bodies |
R1908 |
T9202 |
T9200 |
pobj |
absence,by |
R1909 |
T9203 |
T9202 |
amod |
complete,absence |
R1910 |
T9204 |
T9202 |
prep |
of,absence |
R1911 |
T9023 |
T9021 |
amod |
nuclear,bodies |
R1912 |
T9205 |
T9206 |
nmod |
CAF,signal |
R1913 |
T9206 |
T9204 |
pobj |
signal,of |
R1914 |
T9207 |
T9205 |
punct |
-,CAF |
R1915 |
T9024 |
T9021 |
punct |
", ",bodies |
R1916 |
T9208 |
T9205 |
nummod |
1,CAF |
R1917 |
T9209 |
T9206 |
compound |
IF,signal |
R1918 |
T9210 |
T9197 |
punct |
),cells |
R1919 |
T9025 |
T9026 |
dep |
which,are |
R1920 |
T9211 |
T9212 |
advmod |
still,active |
R1921 |
T9212 |
T9182 |
oprd |
active,appear |
R1922 |
T9213 |
T9212 |
prep |
for,active |
R1923 |
T9214 |
T9213 |
pobj |
replication,for |
R1924 |
T9215 |
T9182 |
punct |
", ",appear |
R1925 |
T9216 |
T9217 |
mark |
as,revealed |
R1926 |
T9026 |
T9021 |
relcl |
are,bodies |
R1927 |
T9217 |
T9182 |
advcl |
revealed,appear |
R1928 |
T9218 |
T9217 |
agent |
by,revealed |
R1929 |
T9219 |
T9218 |
pobj |
incorporation,by |
R1930 |
T9027 |
T9028 |
advmod |
well,characterized |
R1931 |
T9220 |
T9219 |
prep |
of,incorporation |
R1932 |
T9221 |
T9222 |
det |
the,analog |
R1933 |
T9028 |
T9030 |
amod |
characterized,structures |
R1934 |
T9222 |
T9220 |
pobj |
analog,of |
R1935 |
T9223 |
T9222 |
compound |
thymidine,analog |
R1936 |
T9224 |
T9222 |
appos |
bromodeoxyuridine,analog |
R1937 |
T9225 |
T9224 |
punct |
(,bromodeoxyuridine |
R1938 |
T9029 |
T9028 |
punct |
-,characterized |
R1939 |
T9226 |
T9224 |
appos |
BrdU,bromodeoxyuridine |
R1940 |
T9227 |
T9219 |
punct |
),incorporation |
R1941 |
T9228 |
T9229 |
punct |
(,3A |
R1942 |
T9030 |
T9026 |
attr |
structures,are |
R1943 |
T9229 |
T9219 |
parataxis |
3A,incorporation |
R1944 |
T9230 |
T9229 |
compound |
Figure,3A |
R1945 |
T9031 |
T9030 |
amod |
subnuclear,structures |
R1946 |
T9231 |
T9229 |
punct |
),3A |
R1947 |
T9232 |
T9219 |
punct |
", ",incorporation |
R1948 |
T9233 |
T9234 |
det |
the,pattern |
R1949 |
T9032 |
T9033 |
punct |
[,22 |
R1950 |
T9234 |
T9219 |
conj |
pattern,incorporation |
R1951 |
T9235 |
T9234 |
nmod |
PCNA,pattern |
R1952 |
T9236 |
T9235 |
punct |
(,PCNA |
R1953 |
T9033 |
T9003 |
parataxis |
22,used |
R1954 |
T9237 |
T9238 |
amod |
proliferating,antigen |
R1955 |
T9238 |
T9235 |
appos |
antigen,PCNA |
R1956 |
T9239 |
T9238 |
nmod |
cell,antigen |
R1957 |
T9034 |
T9033 |
punct |
],22 |
R1958 |
T9240 |
T9238 |
amod |
nuclear,antigen |
R1959 |
T9241 |
T9234 |
punct |
),pattern |
R1960 |
T9242 |
T9243 |
punct |
(,3B |
R1961 |
T9035 |
T9003 |
punct |
.,used |
R1962 |
T9243 |
T9234 |
parataxis |
3B,pattern |
R1963 |
T9244 |
T9243 |
compound |
Figure,3B |
R1964 |
T9245 |
T9243 |
punct |
),3B |
R1965 |
T9246 |
T9234 |
punct |
", ",pattern |
R1966 |
T9037 |
T9038 |
nsubj |
We,detect |
R1967 |
T9247 |
T9234 |
cc |
and,pattern |
R1968 |
T9248 |
T9249 |
compound |
flow,analysis |
R1969 |
T9249 |
T9234 |
conj |
analysis,pattern |
R1970 |
T9250 |
T9249 |
compound |
cytometry,analysis |
R1971 |
T9039 |
T9038 |
aux |
could,detect |
R1972 |
T9251 |
T9252 |
punct |
(,3C |
R1973 |
T9252 |
T9249 |
parataxis |
3C,analysis |
R1974 |
T9253 |
T9252 |
compound |
Figure,3C |
R1975 |
T9254 |
T9252 |
punct |
),3C |
R1976 |
T9255 |
T9179 |
punct |
.,show |
R1977 |
T9257 |
T9258 |
advmod |
Hence,persists |
R1978 |
T9259 |
T9258 |
punct |
", ",persists |
R1979 |
T9040 |
T9038 |
neg |
not,detect |
R1980 |
T9260 |
T9258 |
prep |
in,persists |
R1981 |
T9261 |
T9262 |
det |
these,cells |
R1982 |
T9262 |
T9260 |
pobj |
cells,in |
R1983 |
T9263 |
T9258 |
punct |
", ",persists |
R1984 |
T9041 |
T9042 |
det |
any,difference |
R1985 |
T9264 |
T9265 |
compound |
DNA,replication |
R1986 |
T9265 |
T9258 |
nsubj |
replication,persists |
R1987 |
T9266 |
T9258 |
prep |
despite,persists |
R1988 |
T9042 |
T9038 |
dobj |
difference,detect |
R1989 |
T9267 |
T9268 |
det |
the,changes |
R1990 |
T9268 |
T9266 |
pobj |
changes,despite |
R1991 |
T9269 |
T9268 |
amod |
drastic,changes |
R1992 |
T9043 |
T9042 |
amod |
significant,difference |
R1993 |
T9270 |
T9268 |
prep |
in,changes |
R1994 |
T9271 |
T9272 |
compound |
heterochromatin,organization |
R1995 |
T9272 |
T9270 |
pobj |
organization,in |
R1996 |
T9044 |
T9042 |
prep |
in,difference |
R1997 |
T9273 |
T9258 |
punct |
.,persists |
R1998 |
T9275 |
T9276 |
advmod |
However,cease |
R1999 |
T9045 |
T9046 |
det |
the,distribution |
R2000 |
T9046 |
T9044 |
pobj |
distribution,in |
R2001 |
T9047 |
T9046 |
cc |
and,distribution |
R2002 |
T9277 |
T9276 |
punct |
", ",cease |
R2003 |
T9048 |
T9046 |
conj |
aspect,distribution |
R2004 |
T9278 |
T9279 |
nummod |
24,h |
R2005 |
T9279 |
T9280 |
npadvmod |
h,later |
R2006 |
T9280 |
T9276 |
advmod |
later,cease |
R2007 |
T9049 |
T9046 |
prep |
of,distribution |
R2008 |
T9281 |
T9276 |
punct |
", ",cease |
R2009 |
T9282 |
T9283 |
compound |
ES,cells |
R2010 |
T9283 |
T9276 |
nsubj |
cells,cease |
R2011 |
T9284 |
T9285 |
aux |
to,proliferate |
R2012 |
T9050 |
T9051 |
nmod |
PML,bodies |
R2013 |
T9285 |
T9276 |
xcomp |
proliferate,cease |
R2014 |
T9286 |
T9276 |
cc |
and,cease |
R2015 |
T9051 |
T9049 |
pobj |
bodies,of |
R2016 |
T9287 |
T9276 |
conj |
die,cease |
R2017 |
T9288 |
T9276 |
punct |
", ",cease |
R2018 |
T9289 |
T9276 |
advcl |
revealing,cease |
R2019 |
T9290 |
T9291 |
mark |
that,required |
R2020 |
T9052 |
T9051 |
amod |
nuclear,bodies |
R2021 |
T9053 |
T9051 |
prep |
between,bodies |
R2022 |
T9291 |
T9289 |
ccomp |
required,revealing |
R2023 |
T9292 |
T9291 |
nsubjpass |
p150CAF,required |
R2024 |
T9054 |
T9055 |
nmod |
control,cells |
R2025 |
T9293 |
T9292 |
punct |
-,p150CAF |
R2026 |
T9294 |
T9292 |
nummod |
1,p150CAF |
R2027 |
T9295 |
T9291 |
auxpass |
is,required |
R2028 |
T9296 |
T9291 |
prep |
for,required |
R2029 |
T9297 |
T9298 |
det |
an,process |
R2030 |
T9298 |
T9296 |
pobj |
process,for |
R2031 |
T9299 |
T9298 |
amod |
essential,process |
R2032 |
T9055 |
T9053 |
pobj |
cells,between |
R2033 |
T9300 |
T9298 |
amod |
cellular,process |
R2034 |
T9301 |
T9291 |
punct |
", ",required |
R2035 |
T9302 |
T9291 |
prep |
as,required |
R2036 |
T9056 |
T9054 |
cc |
and,control |
R2037 |
T9303 |
T9302 |
prep |
in,as |
R2038 |
T9304 |
T9303 |
pobj |
embryos,in |
R2039 |
T9305 |
T9276 |
punct |
.,cease |
R2040 |
T9057 |
T9058 |
npadvmod |
p150CAF,depleted |
R2041 |
T9307 |
T9308 |
advmod |
Altogether,demonstrate |
R2042 |
T9058 |
T9054 |
conj |
depleted,control |
R2043 |
T9309 |
T9308 |
punct |
", ",demonstrate |
R2044 |
T9310 |
T9311 |
poss |
our,data |
R2045 |
T9059 |
T9057 |
punct |
-,p150CAF |
R2046 |
T9311 |
T9308 |
nsubj |
data,demonstrate |
R2047 |
T9312 |
T9313 |
mark |
that,alters |
R2048 |
T9313 |
T9308 |
ccomp |
alters,demonstrate |
R2049 |
T9060 |
T9057 |
nummod |
1,p150CAF |
R2050 |
T9314 |
T9313 |
nsubj |
loss,alters |
R2051 |
T9315 |
T9314 |
prep |
of,loss |
R2052 |
T9316 |
T9317 |
nmod |
p150CAF,function |
R2053 |
T9061 |
T9058 |
punct |
-,depleted |
R2054 |
T9317 |
T9315 |
pobj |
function,of |
R2055 |
T9318 |
T9316 |
punct |
-,p150CAF |
R2056 |
T9319 |
T9316 |
nummod |
1,p150CAF |
R2057 |
T9062 |
T9055 |
compound |
ES,cells |
R2058 |
T9320 |
T9314 |
prep |
in,loss |
R2059 |
T9321 |
T9322 |
compound |
ES,cells |
R2060 |
T9322 |
T9320 |
pobj |
cells,in |
R2061 |
T9063 |
T9064 |
punct |
(,2D |
R2062 |
T9323 |
T9322 |
cc |
and,cells |
R2063 |
T9324 |
T9325 |
amod |
early,embryos |
R2064 |
T9325 |
T9322 |
conj |
embryos,cells |
R2065 |
T9064 |
T9038 |
parataxis |
2D,detect |
R2066 |
T9326 |
T9313 |
advmod |
first,alters |
R2067 |
T9327 |
T9328 |
det |
the,organization |
R2068 |
T9328 |
T9313 |
dobj |
organization,alters |
R2069 |
T9382 |
T9379 |
amod |
chromosomal,regions |
R2070 |
T9329 |
T9328 |
prep |
of,organization |
R2071 |
T9330 |
T9329 |
pobj |
heterochromatin,of |
R2072 |
T9331 |
T9328 |
prep |
in,organization |
R2073 |
T9332 |
T9333 |
det |
the,nucleus |
R2074 |
T9333 |
T9331 |
pobj |
nucleus,in |
R2075 |
T9334 |
T9313 |
cc |
and,alters |
R2076 |
T9383 |
T9379 |
punct |
", ",regions |
R2077 |
T9335 |
T9313 |
punct |
", ",alters |
R2078 |
T9336 |
T9337 |
prep |
in,cycle |
R2079 |
T9337 |
T9313 |
conj |
cycle,alters |
R2080 |
T9338 |
T9339 |
det |
a,step |
R2081 |
T9339 |
T9336 |
pobj |
step,in |
R2082 |
T9340 |
T9339 |
amod |
subsequent,step |
R2083 |
T9341 |
T9337 |
punct |
", ",cycle |
R2084 |
T9342 |
T9337 |
compound |
cell,cycle |
R2085 |
T9384 |
T9385 |
dep |
which,composed |
R2086 |
T9343 |
T9337 |
cc |
and,cycle |
R2087 |
T9344 |
T9337 |
conj |
viability,cycle |
R2088 |
T9345 |
T9308 |
punct |
.,demonstrate |
R2089 |
T9385 |
T9379 |
relcl |
composed,regions |
R2090 |
T9347 |
T9348 |
prep |
In,performed |
R2091 |
T9349 |
T9347 |
pobj |
order,In |
R2092 |
T9350 |
T9351 |
aux |
to,characterize |
R2093 |
T9386 |
T9385 |
prep |
in,composed |
R2094 |
T9351 |
T9349 |
acl |
characterize,order |
R2095 |
T9352 |
T9351 |
advmod |
better,characterize |
R2096 |
T9353 |
T9354 |
det |
the,defects |
R2097 |
T9387 |
T9386 |
pobj |
mouse,in |
R2098 |
T9354 |
T9351 |
dobj |
defects,characterize |
R2099 |
T9355 |
T9354 |
prep |
in,defects |
R2100 |
T9388 |
T9385 |
auxpass |
are,composed |
R2101 |
T9389 |
T9385 |
advmod |
mainly,composed |
R2102 |
T9356 |
T9357 |
compound |
heterochromatin,organization |
R2103 |
T9390 |
T9385 |
prep |
of,composed |
R2104 |
T9357 |
T9355 |
pobj |
organization,in |
R2105 |
T9358 |
T9348 |
punct |
", ",performed |
R2106 |
T9359 |
T9348 |
nsubj |
we,performed |
R2107 |
T9391 |
T9392 |
amod |
large,blocks |
R2108 |
T9360 |
T9361 |
nummod |
two,color |
R2109 |
T9361 |
T9363 |
nmod |
color,experiments |
R2110 |
T9362 |
T9361 |
punct |
-,color |
R2111 |
T9392 |
T9390 |
pobj |
blocks,of |
R2112 |
T9363 |
T9348 |
dobj |
experiments,performed |
R2113 |
T9364 |
T9363 |
nmod |
DNA,experiments |
R2114 |
T9365 |
T9366 |
nmod |
fluorescence,hybridization |
R2115 |
T9393 |
T9392 |
prep |
of,blocks |
R2116 |
T9366 |
T9363 |
nmod |
hybridization,experiments |
R2117 |
T9367 |
T9368 |
advmod |
in,situ |
R2118 |
T9368 |
T9366 |
amod |
situ,hybridization |
R2119 |
T9369 |
T9366 |
punct |
(,hybridization |
R2120 |
T9394 |
T9395 |
amod |
major,repeats |
R2121 |
T9370 |
T9366 |
appos |
FISH,hybridization |
R2122 |
T9371 |
T9363 |
punct |
),experiments |
R2123 |
T9372 |
T9373 |
aux |
to,reveal |
R2124 |
T9395 |
T9393 |
pobj |
repeats,of |
R2125 |
T9373 |
T9348 |
advcl |
reveal,performed |
R2126 |
T9374 |
T9375 |
det |
the,distribution |
R2127 |
T9396 |
T9394 |
cc |
and,major |
R2128 |
T9375 |
T9373 |
dobj |
distribution,reveal |
R2129 |
T9376 |
T9375 |
amod |
spatial,distribution |
R2130 |
T9377 |
T9375 |
prep |
of,distribution |
R2131 |
T9378 |
T9379 |
amod |
pericentric,regions |
R2132 |
T9397 |
T9394 |
conj |
minor,major |
R2133 |
T9379 |
T9377 |
pobj |
regions,of |
R2134 |
T9380 |
T9378 |
cc |
and,pericentric |
R2135 |
T9381 |
T9378 |
conj |
centric,pericentric |
R2136 |
T9398 |
T9395 |
compound |
satellite,repeats |
R2137 |
T9399 |
T9385 |
punct |
", ",composed |
R2138 |
T9400 |
T9385 |
advmod |
respectively,composed |
R2139 |
T9401 |
T9402 |
punct |
[,20 |
R2140 |
T9488 |
T9486 |
punct |
),4B |
R2141 |
T9489 |
T9459 |
punct |
.,observed |
R2142 |
T9402 |
T9348 |
parataxis |
20,performed |
R2143 |
T9491 |
T9492 |
amod |
Individual,domains |
R2144 |
T9403 |
T9402 |
punct |
],20 |
R2145 |
T9492 |
T9494 |
nsubjpass |
domains,found |
R2146 |
T9493 |
T9492 |
amod |
pericentric,domains |
R2147 |
T9404 |
T9348 |
punct |
.,performed |
R2148 |
T9495 |
T9492 |
prep |
from,domains |
R2149 |
T9496 |
T9497 |
amod |
single,chromosomes |
R2150 |
T9497 |
T9495 |
pobj |
chromosomes,from |
R2151 |
T9498 |
T9494 |
auxpass |
are,found |
R2152 |
T9406 |
T9407 |
amod |
Pericentric,domains |
R2153 |
T9499 |
T9494 |
advmod |
now,found |
R2154 |
T9500 |
T9501 |
preconj |
either,isolated |
R2155 |
T9501 |
T9494 |
oprd |
isolated,found |
R2156 |
T9502 |
T9501 |
cc |
or,isolated |
R2157 |
T9407 |
T9408 |
nsubj |
domains,form |
R2158 |
T9503 |
T9501 |
conj |
aggregated,isolated |
R2159 |
T9504 |
T9501 |
prep |
in,isolated |
R2160 |
T9409 |
T9407 |
prep |
from,domains |
R2161 |
T9505 |
T9506 |
det |
a,pattern |
R2162 |
T9506 |
T9504 |
pobj |
pattern,in |
R2163 |
T9507 |
T9508 |
advmod |
less,dense |
R2164 |
T9508 |
T9506 |
amod |
dense,pattern |
R2165 |
T9410 |
T9411 |
amod |
different,chromosomes |
R2166 |
T9509 |
T9508 |
prep |
than,dense |
R2167 |
T9510 |
T9511 |
det |
the,clusters |
R2168 |
T9511 |
T9509 |
pobj |
clusters,than |
R2169 |
T9411 |
T9409 |
pobj |
chromosomes,from |
R2170 |
T9512 |
T9511 |
amod |
regular,clusters |
R2171 |
T9513 |
T9511 |
acl |
observed,clusters |
R2172 |
T9514 |
T9513 |
prep |
in,observed |
R2173 |
T9412 |
T9408 |
dobj |
clusters,form |
R2174 |
T9515 |
T9516 |
compound |
control,cells |
R2175 |
T9516 |
T9514 |
pobj |
cells,in |
R2176 |
T9517 |
T9518 |
punct |
(,4B |
R2177 |
T9413 |
T9412 |
punct |
", ",clusters |
R2178 |
T9518 |
T9494 |
parataxis |
4B,found |
R2179 |
T9519 |
T9518 |
compound |
Figure,4B |
R2180 |
T9520 |
T9518 |
punct |
),4B |
R2181 |
T9521 |
T9494 |
punct |
.,found |
R2182 |
T9523 |
T9524 |
amod |
Such,aggregates |
R2183 |
T9414 |
T9415 |
dep |
which,revealed |
R2184 |
T9524 |
T9525 |
nsubjpass |
aggregates,revealed |
R2185 |
T9526 |
T9524 |
punct |
", ",aggregates |
R2186 |
T9415 |
T9412 |
relcl |
revealed,clusters |
R2187 |
T9527 |
T9528 |
dep |
which,found |
R2188 |
T9528 |
T9524 |
relcl |
found,aggregates |
R2189 |
T9529 |
T9528 |
auxpass |
are,found |
R2190 |
T9416 |
T9415 |
auxpass |
are,revealed |
R2191 |
T9530 |
T9528 |
advmod |
often,found |
R2192 |
T9531 |
T9528 |
prep |
at,found |
R2193 |
T9532 |
T9533 |
det |
the,periphery |
R2194 |
T9417 |
T9415 |
prep |
by,revealed |
R2195 |
T9533 |
T9531 |
pobj |
periphery,at |
R2196 |
T9534 |
T9533 |
amod |
nuclear,periphery |
R2197 |
T9535 |
T9525 |
punct |
", ",revealed |
R2198 |
T9418 |
T9417 |
pobj |
FISH,by |
R2199 |
T9536 |
T9525 |
auxpass |
are,revealed |
R2200 |
T9537 |
T9525 |
advmod |
also,revealed |
R2201 |
T9538 |
T9525 |
prep |
by,revealed |
R2202 |
T9419 |
T9415 |
prep |
as,revealed |
R2203 |
T9539 |
T9540 |
compound |
DAPI,staining |
R2204 |
T9540 |
T9538 |
pobj |
staining,by |
R2205 |
T9541 |
T9525 |
prep |
as,revealed |
R2206 |
T9420 |
T9421 |
amod |
large,spots |
R2207 |
T9542 |
T9543 |
det |
a,signature |
R2208 |
T9543 |
T9541 |
pobj |
signature,as |
R2209 |
T9544 |
T9543 |
amod |
typical,signature |
R2210 |
T9421 |
T9419 |
pobj |
spots,as |
R2211 |
T9545 |
T9543 |
prep |
of,signature |
R2212 |
T9546 |
T9545 |
pobj |
p150CAF,of |
R2213 |
T9547 |
T9546 |
punct |
-,p150CAF |
R2214 |
T9422 |
T9423 |
dep |
that,coincide |
R2215 |
T9548 |
T9546 |
nummod |
1,p150CAF |
R2216 |
T9423 |
T9421 |
relcl |
coincide,spots |
R2217 |
T9549 |
T9546 |
appos |
loss,p150CAF |
R2218 |
T9550 |
T9549 |
punct |
-,loss |
R2219 |
T9424 |
T9423 |
prep |
with,coincide |
R2220 |
T9551 |
T9549 |
prep |
of,loss |
R2221 |
T9552 |
T9551 |
punct |
-,of |
R2222 |
T9425 |
T9426 |
npadvmod |
DAPI,dense |
R2223 |
T9553 |
T9551 |
pobj |
function,of |
R2224 |
T9554 |
T9525 |
punct |
.,revealed |
R2225 |
T9556 |
T9557 |
nsubj |
Quantification,revealed |
R2226 |
T9426 |
T9428 |
amod |
dense,foci |
R2227 |
T9558 |
T9556 |
prep |
of,Quantification |
R2228 |
T9559 |
T9558 |
pobj |
fluorescence,of |
R2229 |
T9427 |
T9426 |
punct |
-,dense |
R2230 |
T9560 |
T9556 |
prep |
along,Quantification |
R2231 |
T9561 |
T9562 |
det |
a,line |
R2232 |
T9428 |
T9424 |
pobj |
foci,with |
R2233 |
T9562 |
T9560 |
pobj |
line,along |
R2234 |
T9563 |
T9564 |
advmod |
randomly,drawn |
R2235 |
T9564 |
T9562 |
acl |
drawn,line |
R2236 |
T9565 |
T9564 |
prep |
across,drawn |
R2237 |
T9566 |
T9567 |
det |
the,nucleus |
R2238 |
T9567 |
T9565 |
pobj |
nucleus,across |
R2239 |
T9568 |
T9569 |
amod |
lower,intensity |
R2240 |
T9429 |
T9423 |
prep |
in,coincide |
R2241 |
T9569 |
T9557 |
dobj |
intensity,revealed |
R2242 |
T9570 |
T9569 |
compound |
fluorescence,intensity |
R2243 |
T9571 |
T9569 |
cc |
and,intensity |
R2244 |
T9430 |
T9431 |
det |
the,nucleus |
R2245 |
T9572 |
T9573 |
det |
a,distribution |
R2246 |
T9573 |
T9569 |
conj |
distribution,intensity |
R2247 |
T9574 |
T9573 |
amod |
broader,distribution |
R2248 |
T9431 |
T9429 |
pobj |
nucleus,in |
R2249 |
T9575 |
T9573 |
prep |
of,distribution |
R2250 |
T9576 |
T9575 |
pobj |
DAPI,of |
R2251 |
T9577 |
T9576 |
cc |
and,DAPI |
R2252 |
T9578 |
T9579 |
amod |
major,signals |
R2253 |
T9432 |
T9431 |
compound |
interphase,nucleus |
R2254 |
T9579 |
T9576 |
conj |
signals,DAPI |
R2255 |
T9580 |
T9579 |
compound |
satellite,signals |
R2256 |
T9433 |
T9434 |
punct |
(,4A |
R2257 |
T9581 |
T9579 |
compound |
hybridization,signals |
R2258 |
T9582 |
T9557 |
prep |
in,revealed |
R2259 |
T9583 |
T9584 |
npadvmod |
p150CAF,depleted |
R2260 |
T9584 |
T9588 |
amod |
depleted,cells |
R2261 |
T9434 |
T9423 |
parataxis |
4A,coincide |
R2262 |
T9585 |
T9583 |
punct |
-,p150CAF |
R2263 |
T9586 |
T9583 |
nummod |
1,p150CAF |
R2264 |
T9587 |
T9584 |
punct |
-,depleted |
R2265 |
T9435 |
T9434 |
compound |
Figure,4A |
R2266 |
T9588 |
T9582 |
pobj |
cells,in |
R2267 |
T9589 |
T9590 |
punct |
(,4C |
R2268 |
T9590 |
T9557 |
parataxis |
4C,revealed |
R2269 |
T9436 |
T9434 |
punct |
),4A |
R2270 |
T9591 |
T9590 |
compound |
Figure,4C |
R2271 |
T9592 |
T9590 |
cc |
and,4C |
R2272 |
T9593 |
T9590 |
conj |
4D,4C |
R2273 |
T9437 |
T9408 |
punct |
.,form |
R2274 |
T9439 |
T9440 |
prep |
At,form |
R2275 |
T9594 |
T9590 |
punct |
),4C |
R2276 |
T9441 |
T9442 |
det |
the,periphery |
R2277 |
T9595 |
T9557 |
punct |
", ",revealed |
R2278 |
T9596 |
T9557 |
advcl |
indicating,revealed |
R2279 |
T9597 |
T9596 |
dobj |
decondensation,indicating |
R2280 |
T9598 |
T9597 |
prep |
of,decondensation |
R2281 |
T9442 |
T9439 |
pobj |
periphery,At |
R2282 |
T9599 |
T9600 |
amod |
pericentric,domains |
R2283 |
T9600 |
T9598 |
pobj |
domains,of |
R2284 |
T9601 |
T9600 |
compound |
heterochromatin,domains |
R2285 |
T9602 |
T9557 |
punct |
.,revealed |
R2286 |
T9604 |
T9605 |
prep |
In,showed |
R2287 |
T9443 |
T9442 |
prep |
of,periphery |
R2288 |
T9606 |
T9604 |
pobj |
contrast,In |
R2289 |
T9607 |
T9605 |
punct |
", ",showed |
R2290 |
T9608 |
T9609 |
amod |
centric,domains |
R2291 |
T9444 |
T9445 |
det |
each,domain |
R2292 |
T9609 |
T9605 |
nsubj |
domains,showed |
R2293 |
T9610 |
T9611 |
det |
an,intensity |
R2294 |
T9445 |
T9443 |
pobj |
domain,of |
R2295 |
T9611 |
T9605 |
dobj |
intensity,showed |
R2296 |
T9612 |
T9611 |
cc |
and,intensity |
R2297 |
T9613 |
T9611 |
conj |
shape,intensity |
R2298 |
T9446 |
T9445 |
amod |
pericentric,domain |
R2299 |
T9614 |
T9611 |
amod |
similar,intensity |
R2300 |
T9615 |
T9614 |
prep |
to,similar |
R2301 |
T9616 |
T9617 |
compound |
control,cells |
R2302 |
T9447 |
T9440 |
punct |
", ",form |
R2303 |
T9617 |
T9615 |
pobj |
cells,to |
R2304 |
T9618 |
T9605 |
punct |
", ",showed |
R2305 |
T9619 |
T9605 |
advcl |
suggesting,showed |
R2306 |
T9448 |
T9449 |
amod |
centric,regions |
R2307 |
T9620 |
T9621 |
mark |
that,remain |
R2308 |
T9621 |
T9619 |
ccomp |
remain,suggesting |
R2309 |
T9622 |
T9621 |
nsubj |
they,remain |
R2310 |
T9449 |
T9440 |
nsubj |
regions,form |
R2311 |
T9623 |
T9621 |
acomp |
unaffected,remain |
R2312 |
T9624 |
T9623 |
prep |
by,unaffected |
R2313 |
T9625 |
T9626 |
nmod |
p150CAF,depletion |
R2314 |
T9450 |
T9451 |
amod |
individual,entities |
R2315 |
T9626 |
T9624 |
pobj |
depletion,by |
R2316 |
T9627 |
T9625 |
punct |
-,p150CAF |
R2317 |
T9628 |
T9625 |
nummod |
1,p150CAF |
R2318 |
T9451 |
T9440 |
dobj |
entities,form |
R2319 |
T9629 |
T9630 |
punct |
(,4C |
R2320 |
T9630 |
T9605 |
parataxis |
4C,showed |
R2321 |
T9631 |
T9630 |
compound |
Figure,4C |
R2322 |
T9452 |
T9453 |
punct |
(,4A |
R2323 |
T9632 |
T9630 |
cc |
and,4C |
R2324 |
T9453 |
T9440 |
parataxis |
4A,form |
R2325 |
T9633 |
T9630 |
conj |
4D,4C |
R2326 |
T9634 |
T9630 |
punct |
),4C |
R2327 |
T9635 |
T9605 |
punct |
.,showed |
R2328 |
T9454 |
T9453 |
compound |
Figure,4A |
R2329 |
T9637 |
T9638 |
prep |
In,show |
R2330 |
T9455 |
T9453 |
punct |
),4A |
R2331 |
T9639 |
T9637 |
pobj |
conclusion,In |
R2332 |
T9640 |
T9638 |
punct |
", ",show |
R2333 |
T9641 |
T9642 |
det |
these,results |
R2334 |
T9642 |
T9638 |
nsubj |
results,show |
R2335 |
T9456 |
T9440 |
punct |
.,form |
R2336 |
T9643 |
T9644 |
mark |
that,required |
R2337 |
T9644 |
T9638 |
ccomp |
required,show |
R2338 |
T9645 |
T9644 |
nsubjpass |
p150CAF,required |
R2339 |
T9646 |
T9645 |
punct |
-,p150CAF |
R2340 |
T9647 |
T9645 |
nummod |
1,p150CAF |
R2341 |
T9458 |
T9459 |
prep |
In,observed |
R2342 |
T9648 |
T9644 |
auxpass |
is,required |
R2343 |
T9649 |
T9644 |
prep |
for,required |
R2344 |
T9650 |
T9651 |
det |
the,condensation |
R2345 |
T9651 |
T9649 |
pobj |
condensation,for |
R2346 |
T9652 |
T9651 |
amod |
proper,condensation |
R2347 |
T9460 |
T9461 |
nmod |
p150CAF,cells |
R2348 |
T9653 |
T9651 |
cc |
and,condensation |
R2349 |
T9654 |
T9651 |
conj |
clustering,condensation |
R2350 |
T9655 |
T9651 |
prep |
of,condensation |
R2351 |
T9656 |
T9657 |
amod |
pericentric,domains |
R2352 |
T9461 |
T9458 |
pobj |
cells,In |
R2353 |
T9657 |
T9655 |
pobj |
domains,of |
R2354 |
T9658 |
T9657 |
compound |
heterochromatin,domains |
R2355 |
T9462 |
T9460 |
punct |
-,p150CAF |
R2356 |
T9659 |
T9638 |
punct |
.,show |
R2357 |
T9661 |
T9662 |
prep |
Given,wondered |
R2358 |
T9463 |
T9460 |
nummod |
1,p150CAF |
R2359 |
T9663 |
T9664 |
det |
the,role |
R2360 |
T9664 |
T9661 |
pobj |
role,Given |
R2361 |
T9464 |
T9465 |
npadvmod |
RNAi,depleted |
R2362 |
T9665 |
T9664 |
amod |
known,role |
R2363 |
T9666 |
T9664 |
prep |
of,role |
R2364 |
T9667 |
T9666 |
pobj |
CAF,of |
R2365 |
T9465 |
T9461 |
amod |
depleted,cells |
R2366 |
T9668 |
T9667 |
punct |
-,CAF |
R2367 |
T9669 |
T9667 |
nummod |
1,CAF |
R2368 |
T9670 |
T9664 |
prep |
in,role |
R2369 |
T9466 |
T9465 |
punct |
-,depleted |
R2370 |
T9671 |
T9672 |
det |
the,deposition |
R2371 |
T9672 |
T9670 |
pobj |
deposition,in |
R2372 |
T9673 |
T9672 |
prep |
of,deposition |
R2373 |
T9674 |
T9675 |
compound |
histone,H3.1 |
R2374 |
T9467 |
T9459 |
punct |
", ",observed |
R2375 |
T9675 |
T9673 |
pobj |
H3.1,of |
R2376 |
T9676 |
T9675 |
cc |
and,H3.1 |
R2377 |
T9677 |
T9675 |
conj |
H4,H3.1 |
R2378 |
T9468 |
T9459 |
nsubj |
we,observed |
R2379 |
T9678 |
T9675 |
acl |
associated,H3.1 |
R2380 |
T9679 |
T9678 |
prep |
with,associated |
R2381 |
T9680 |
T9681 |
compound |
DNA,synthesis |
R2382 |
T9469 |
T9470 |
det |
a,disruption |
R2383 |
T9681 |
T9679 |
pobj |
synthesis,with |
R2384 |
T9682 |
T9683 |
punct |
[,12 |
R2385 |
T9683 |
T9664 |
parataxis |
12,role |
R2386 |
T9470 |
T9459 |
dobj |
disruption,observed |
R2387 |
T9684 |
T9683 |
nummod |
11,12 |
R2388 |
T9685 |
T9683 |
punct |
",",12 |
R2389 |
T9686 |
T9683 |
punct |
],12 |
R2390 |
T9687 |
T9662 |
punct |
", ",wondered |
R2391 |
T9688 |
T9662 |
nsubj |
we,wondered |
R2392 |
T9689 |
T9690 |
mark |
whether,reflect |
R2393 |
T9690 |
T9662 |
ccomp |
reflect,wondered |
R2394 |
T9691 |
T9692 |
det |
this,defect |
R2395 |
T9471 |
T9470 |
prep |
of,disruption |
R2396 |
T9692 |
T9690 |
nsubj |
defect,reflect |
R2397 |
T9693 |
T9692 |
prep |
in,defect |
R2398 |
T9694 |
T9695 |
amod |
higher,order |
R2399 |
T9472 |
T9473 |
amod |
pericentric,clusters |
R2400 |
T9695 |
T9697 |
compound |
order,organization |
R2401 |
T9696 |
T9695 |
punct |
-,order |
R2402 |
T9697 |
T9693 |
pobj |
organization,in |
R2403 |
T9698 |
T9697 |
compound |
chromatin,organization |
R2404 |
T9473 |
T9471 |
pobj |
clusters,of |
R2405 |
T9699 |
T9690 |
aux |
could,reflect |
R2406 |
T9474 |
T9473 |
compound |
heterochromatin,clusters |
R2407 |
T9475 |
T9476 |
dep |
that,coincides |
R2408 |
T9476 |
T9470 |
relcl |
coincides,disruption |
R2409 |
T9477 |
T9476 |
prep |
with,coincides |
R2410 |
T9700 |
T9701 |
det |
an,organization |
R2411 |
T9701 |
T9690 |
dobj |
organization,reflect |
R2412 |
T9478 |
T9479 |
det |
the,disappearance |
R2413 |
T9702 |
T9701 |
amod |
aberrant,organization |
R2414 |
T9703 |
T9701 |
amod |
nucleosomal,organization |
R2415 |
T9704 |
T9662 |
punct |
.,wondered |
R2416 |
T9479 |
T9477 |
pobj |
disappearance,with |
R2417 |
T9706 |
T9707 |
advcl |
Using,observe |
R2418 |
T9480 |
T9479 |
prep |
of,disappearance |
R2419 |
T9708 |
T9706 |
dobj |
DNase,Using |
R2420 |
T9709 |
T9708 |
nummod |
I,DNase |
R2421 |
T9481 |
T9482 |
npadvmod |
DAPI,dense |
R2422 |
T9710 |
T9708 |
cc |
and,DNase |
R2423 |
T9711 |
T9712 |
amod |
micrococcal,nuclease |
R2424 |
T9712 |
T9713 |
nmod |
nuclease,assays |
R2425 |
T9482 |
T9484 |
amod |
dense,foci |
R2426 |
T9713 |
T9708 |
conj |
assays,DNase |
R2427 |
T9714 |
T9712 |
punct |
(,nuclease |
R2428 |
T9715 |
T9712 |
appos |
MNase,nuclease |
R2429 |
T9483 |
T9482 |
punct |
-,dense |
R2430 |
T9716 |
T9713 |
punct |
),assays |
R2431 |
T9717 |
T9707 |
punct |
", ",observe |
R2432 |
T9718 |
T9707 |
nsubj |
we,observe |
R2433 |
T9484 |
T9480 |
pobj |
foci,of |
R2434 |
T9719 |
T9707 |
aux |
could,observe |
R2435 |
T9485 |
T9486 |
punct |
(,4B |
R2436 |
T9720 |
T9707 |
neg |
not,observe |
R2437 |
T9486 |
T9459 |
parataxis |
4B,observed |
R2438 |
T9721 |
T9722 |
det |
any,difference |
R2439 |
T9722 |
T9707 |
dobj |
difference,observe |
R2440 |
T9723 |
T9722 |
amod |
significant,difference |
R2441 |
T9724 |
T9722 |
prep |
between,difference |
R2442 |
T9725 |
T9726 |
nmod |
control,cells |
R2443 |
T9487 |
T9486 |
compound |
Figure,4B |
R2444 |
T9726 |
T9724 |
pobj |
cells,between |
R2445 |
T9727 |
T9725 |
cc |
and,control |
R2446 |
T9728 |
T9729 |
npadvmod |
p150CAF,depleted |
R2447 |
T9729 |
T9725 |
conj |
depleted,control |
R2448 |
T9806 |
T9807 |
det |
all,conditions |
R2449 |
T9730 |
T9728 |
punct |
-,p150CAF |
R2450 |
T9731 |
T9728 |
nummod |
1,p150CAF |
R2451 |
T9732 |
T9729 |
punct |
-,depleted |
R2452 |
T9733 |
T9726 |
compound |
ES,cells |
R2453 |
T9734 |
T9707 |
prep |
at,observe |
R2454 |
T9735 |
T9736 |
det |
the,level |
R2455 |
T9807 |
T9804 |
pobj |
conditions,In |
R2456 |
T9736 |
T9734 |
pobj |
level,at |
R2457 |
T9737 |
T9736 |
prep |
of,level |
R2458 |
T9738 |
T9739 |
compound |
bulk,chromatin |
R2459 |
T9739 |
T9737 |
pobj |
chromatin,of |
R2460 |
T9808 |
T9805 |
punct |
", ",was |
R2461 |
T9740 |
T9739 |
compound |
genome,chromatin |
R2462 |
T9741 |
T9734 |
cc |
or,at |
R2463 |
T9809 |
T9810 |
det |
the,amount |
R2464 |
T9742 |
T9734 |
conj |
at,at |
R2465 |
T9810 |
T9805 |
nsubj |
amount,was |
R2466 |
T9743 |
T9744 |
amod |
pericentric,repeats |
R2467 |
T9744 |
T9742 |
pobj |
repeats,at |
R2468 |
T9811 |
T9810 |
prep |
of,amount |
R2469 |
T9745 |
T9746 |
punct |
(,Figure |
R2470 |
T9746 |
T9707 |
parataxis |
Figure,observe |
R2471 |
T9747 |
T9746 |
nummod |
5,Figure |
R2472 |
T9748 |
T9746 |
punct |
),Figure |
R2473 |
T9812 |
T9813 |
compound |
histone,H3 |
R2474 |
T9749 |
T9707 |
punct |
.,observe |
R2475 |
T9751 |
T9752 |
prep |
In,compared |
R2476 |
T9813 |
T9811 |
pobj |
H3,of |
R2477 |
T9753 |
T9754 |
det |
a,series |
R2478 |
T9814 |
T9810 |
acl |
remaining,amount |
R2479 |
T9754 |
T9751 |
pobj |
series,In |
R2480 |
T9755 |
T9754 |
amod |
second,series |
R2481 |
T9756 |
T9754 |
prep |
of,series |
R2482 |
T9815 |
T9810 |
acl |
associated,amount |
R2483 |
T9757 |
T9756 |
pobj |
experiments,of |
R2484 |
T9758 |
T9752 |
punct |
", ",compared |
R2485 |
T9759 |
T9752 |
nsubj |
we,compared |
R2486 |
T9816 |
T9815 |
prep |
with,associated |
R2487 |
T9760 |
T9761 |
det |
the,association |
R2488 |
T9761 |
T9752 |
dobj |
association,compared |
R2489 |
T9762 |
T9761 |
prep |
of,association |
R2490 |
T9763 |
T9764 |
compound |
histone,H3 |
R2491 |
T9764 |
T9762 |
pobj |
H3,of |
R2492 |
T9817 |
T9816 |
pobj |
chromatin,with |
R2493 |
T9765 |
T9752 |
prep |
with,compared |
R2494 |
T9766 |
T9765 |
pobj |
chromatin,with |
R2495 |
T9767 |
T9752 |
prep |
in,compared |
R2496 |
T9768 |
T9767 |
pobj |
nuclei,in |
R2497 |
T9769 |
T9768 |
acl |
isolated,nuclei |
R2498 |
T9770 |
T9769 |
prep |
from,isolated |
R2499 |
T9771 |
T9770 |
pobj |
cells,from |
R2500 |
T9772 |
T9771 |
acl |
transfected,cells |
R2501 |
T9818 |
T9805 |
acomp |
indistinguishable,was |
R2502 |
T9773 |
T9772 |
prep |
with,transfected |
R2503 |
T9774 |
T9775 |
nmod |
control,vectors |
R2504 |
T9775 |
T9773 |
pobj |
vectors,with |
R2505 |
T9776 |
T9774 |
cc |
and,control |
R2506 |
T9819 |
T9805 |
prep |
in,was |
R2507 |
T9777 |
T9774 |
conj |
p150CAF,control |
R2508 |
T9778 |
T9777 |
punct |
-,p150CAF |
R2509 |
T9779 |
T9777 |
nummod |
1,p150CAF |
R2510 |
T9820 |
T9821 |
nmod |
control,cells |
R2511 |
T9780 |
T9775 |
compound |
RNAi,vectors |
R2512 |
T9781 |
T9775 |
compound |
plasmid,vectors |
R2513 |
T9782 |
T9752 |
punct |
.,compared |
R2514 |
T9821 |
T9819 |
pobj |
cells,in |
R2515 |
T9784 |
T9785 |
nsubjpass |
Nuclei,incubated |
R2516 |
T9786 |
T9785 |
auxpass |
were,incubated |
R2517 |
T9822 |
T9820 |
cc |
and,control |
R2518 |
T9787 |
T9785 |
prep |
in,incubated |
R2519 |
T9788 |
T9787 |
pobj |
buffers,in |
R2520 |
T9823 |
T9824 |
npadvmod |
p150CAF,depleted |
R2521 |
T9789 |
T9788 |
prep |
with,buffers |
R2522 |
T9790 |
T9791 |
amod |
different,concentrations |
R2523 |
T9791 |
T9789 |
pobj |
concentrations,with |
R2524 |
T9824 |
T9820 |
conj |
depleted,control |
R2525 |
T9792 |
T9791 |
compound |
salt,concentrations |
R2526 |
T9793 |
T9791 |
acl |
ranging,concentrations |
R2527 |
T9794 |
T9793 |
prep |
from,ranging |
R2528 |
T9795 |
T9796 |
nummod |
100,mM |
R2529 |
T9825 |
T9823 |
punct |
-,p150CAF |
R2530 |
T9796 |
T9797 |
compound |
mM,NaCl |
R2531 |
T9797 |
T9794 |
pobj |
NaCl,from |
R2532 |
T9798 |
T9794 |
prep |
to,from |
R2533 |
T9826 |
T9823 |
nummod |
1,p150CAF |
R2534 |
T9799 |
T9800 |
nummod |
1,M |
R2535 |
T9800 |
T9801 |
compound |
M,NaCl |
R2536 |
T9801 |
T9798 |
pobj |
NaCl,to |
R2537 |
T9802 |
T9785 |
punct |
.,incubated |
R2538 |
T9827 |
T9824 |
punct |
-,depleted |
R2539 |
T9804 |
T9805 |
prep |
In,was |
R2540 |
T9828 |
T9821 |
compound |
ES,cells |
R2541 |
T9829 |
T9830 |
punct |
(,data |
R2542 |
T9830 |
T9805 |
meta |
data,was |
R2543 |
T9831 |
T9830 |
amod |
unpublished,data |
R2544 |
T9912 |
T9909 |
parataxis |
25,methylation |
R2545 |
T9913 |
T9912 |
punct |
],25 |
R2546 |
T9914 |
T9906 |
prep |
at,tested |
R2547 |
T9915 |
T9916 |
amod |
major,repeats |
R2548 |
T9832 |
T9830 |
punct |
),data |
R2549 |
T9916 |
T9914 |
pobj |
repeats,at |
R2550 |
T9917 |
T9916 |
compound |
satellite,repeats |
R2551 |
T9918 |
T9906 |
cc |
and,tested |
R2552 |
T9919 |
T9906 |
conj |
found,tested |
R2553 |
T9833 |
T9805 |
punct |
.,was |
R2554 |
T9920 |
T9921 |
det |
no,difference |
R2555 |
T9921 |
T9919 |
dobj |
difference,found |
R2556 |
T9922 |
T9921 |
amod |
significant,difference |
R2557 |
T9923 |
T9921 |
prep |
between,difference |
R2558 |
T9835 |
T9836 |
advmod |
Therefore,are |
R2559 |
T9924 |
T9925 |
nmod |
control,cells |
R2560 |
T9925 |
T9923 |
pobj |
cells,between |
R2561 |
T9926 |
T9924 |
cc |
and,control |
R2562 |
T9927 |
T9928 |
npadvmod |
p150CAF,depleted |
R2563 |
T9928 |
T9924 |
conj |
depleted,control |
R2564 |
T9837 |
T9836 |
punct |
", ",are |
R2565 |
T9929 |
T9927 |
punct |
-,p150CAF |
R2566 |
T9930 |
T9927 |
nummod |
1,p150CAF |
R2567 |
T9931 |
T9928 |
punct |
-,depleted |
R2568 |
T9932 |
T9933 |
punct |
(,Figure |
R2569 |
T9838 |
T9839 |
det |
the,loss |
R2570 |
T9933 |
T9919 |
parataxis |
Figure,found |
R2571 |
T9934 |
T9933 |
nummod |
6,Figure |
R2572 |
T9935 |
T9933 |
punct |
),Figure |
R2573 |
T9839 |
T9836 |
nsubj |
loss,are |
R2574 |
T9936 |
T9906 |
punct |
.,tested |
R2575 |
T9938 |
T9939 |
mark |
As,is |
R2576 |
T9840 |
T9839 |
prep |
of,loss |
R2577 |
T9939 |
T9952 |
advcl |
is,reduced |
R2578 |
T9940 |
T9941 |
det |
the,line |
R2579 |
T9941 |
T9939 |
nsubj |
line,is |
R2580 |
T9841 |
T9840 |
pobj |
clustering,of |
R2581 |
T9942 |
T9941 |
compound |
LTM7,line |
R2582 |
T9943 |
T9941 |
compound |
ES,line |
R2583 |
T9944 |
T9941 |
compound |
cell,line |
R2584 |
T9842 |
T9839 |
cc |
and,loss |
R2585 |
T9945 |
T9946 |
dep |
that,used |
R2586 |
T9946 |
T9941 |
relcl |
used,line |
R2587 |
T9947 |
T9946 |
nsubj |
we,used |
R2588 |
T9948 |
T9946 |
prep |
in,used |
R2589 |
T9843 |
T9839 |
conj |
decondensation,loss |
R2590 |
T9949 |
T9950 |
poss |
our,experiments |
R2591 |
T9950 |
T9948 |
pobj |
experiments,in |
R2592 |
T9951 |
T9950 |
compound |
RNAi,experiments |
R2593 |
T9844 |
T9843 |
prep |
of,decondensation |
R2594 |
T9953 |
T9954 |
det |
a,line |
R2595 |
T9954 |
T9939 |
attr |
line,is |
R2596 |
T9845 |
T9846 |
amod |
pericentric,heterochromatin |
R2597 |
T9955 |
T9954 |
amod |
female,line |
R2598 |
T9956 |
T9957 |
punct |
(,XX |
R2599 |
T9957 |
T9955 |
parataxis |
XX,female |
R2600 |
T9958 |
T9957 |
punct |
),XX |
R2601 |
T9959 |
T9954 |
compound |
cell,line |
R2602 |
T9960 |
T9952 |
punct |
", ",reduced |
R2603 |
T9961 |
T9962 |
compound |
DNA,methylation |
R2604 |
T9962 |
T9952 |
nsubjpass |
methylation,reduced |
R2605 |
T9846 |
T9844 |
pobj |
heterochromatin,of |
R2606 |
T9963 |
T9952 |
auxpass |
is,reduced |
R2607 |
T9964 |
T9952 |
advmod |
globally,reduced |
R2608 |
T9965 |
T9952 |
prep |
in,reduced |
R2609 |
T9847 |
T9836 |
acomp |
unlikely,are |
R2610 |
T9966 |
T9967 |
det |
these,cells |
R2611 |
T9967 |
T9965 |
pobj |
cells,in |
R2612 |
T9968 |
T9969 |
punct |
[,26 |
R2613 |
T9969 |
T9952 |
parataxis |
26,reduced |
R2614 |
T9848 |
T9849 |
aux |
to,be |
R2615 |
T9970 |
T9969 |
punct |
],26 |
R2616 |
T9971 |
T9952 |
punct |
.,reduced |
R2617 |
T9849 |
T9847 |
xcomp |
be,unlikely |
R2618 |
T9973 |
T9974 |
aux |
To,rule |
R2619 |
T9974 |
T9975 |
advcl |
rule,tested |
R2620 |
T9976 |
T9974 |
prt |
out,rule |
R2621 |
T9977 |
T9978 |
det |
the,possibility |
R2622 |
T9978 |
T9974 |
dobj |
possibility,rule |
R2623 |
T9979 |
T9980 |
mark |
that,contribute |
R2624 |
T9850 |
T9851 |
det |
the,consequence |
R2625 |
T9851 |
T9849 |
attr |
consequence,be |
R2626 |
T9980 |
T9978 |
acl |
contribute,possibility |
R2627 |
T9981 |
T9980 |
nsubj |
hypomethylation,contribute |
R2628 |
T9982 |
T9981 |
prep |
of,hypomethylation |
R2629 |
T9983 |
T9984 |
amod |
pericentric,repeats |
R2630 |
T9852 |
T9851 |
prep |
of,consequence |
R2631 |
T9984 |
T9982 |
pobj |
repeats,of |
R2632 |
T9985 |
T9980 |
aux |
might,contribute |
R2633 |
T9986 |
T9980 |
prep |
to,contribute |
R2634 |
T9987 |
T9988 |
det |
the,destabilization |
R2635 |
T9853 |
T9854 |
amod |
severe,defects |
R2636 |
T9988 |
T9986 |
pobj |
destabilization,to |
R2637 |
T9989 |
T9988 |
prep |
of,destabilization |
R2638 |
T9990 |
T9991 |
compound |
heterochromatin,domains |
R2639 |
T9854 |
T9852 |
pobj |
defects,of |
R2640 |
T9991 |
T9989 |
pobj |
domains,of |
R2641 |
T9992 |
T9980 |
prep |
in,contribute |
R2642 |
T9993 |
T9994 |
npadvmod |
p150CAF,depleted |
R2643 |
T9994 |
T9998 |
amod |
depleted,cells |
R2644 |
T9995 |
T9993 |
punct |
-,p150CAF |
R2645 |
T9855 |
T9854 |
prep |
in,defects |
R2646 |
T9996 |
T9993 |
nummod |
1,p150CAF |
R2647 |
T9997 |
T9994 |
punct |
-,depleted |
R2648 |
T9998 |
T9992 |
pobj |
cells,in |
R2649 |
T9999 |
T9998 |
compound |
ES,cells |
R2650 |
T10000 |
T9975 |
punct |
", ",tested |
R2651 |
T10001 |
T9975 |
nsubj |
we,tested |
R2652 |
T10002 |
T10003 |
poss |
our,vector |
R2653 |
T10003 |
T9975 |
dobj |
vector,tested |
R2654 |
T9856 |
T9857 |
amod |
nucleosomal,organization |
R2655 |
T10004 |
T10003 |
compound |
RNAi,vector |
R2656 |
T10005 |
T10003 |
compound |
plasmid,vector |
R2657 |
T10006 |
T9975 |
prep |
in,tested |
R2658 |
T10007 |
T10008 |
det |
a,line |
R2659 |
T9857 |
T9855 |
pobj |
organization,in |
R2660 |
T10008 |
T10006 |
pobj |
line,in |
R2661 |
T10009 |
T10008 |
compound |
XY,line |
R2662 |
T10010 |
T10008 |
compound |
ES,line |
R2663 |
T9858 |
T9836 |
punct |
.,are |
R2664 |
T10011 |
T10008 |
compound |
cell,line |
R2665 |
T10012 |
T9975 |
punct |
", ",tested |
R2666 |
T10013 |
T9975 |
cc |
and,tested |
R2667 |
T9860 |
T9861 |
poss |
Our,findings |
R2668 |
T10014 |
T9975 |
conj |
confirmed,tested |
R2669 |
T10015 |
T10016 |
mark |
that,leads |
R2670 |
T10016 |
T10014 |
ccomp |
leads,confirmed |
R2671 |
T9861 |
T9862 |
nsubj |
findings,showed |
R2672 |
T9863 |
T9864 |
mark |
that,altered |
R2673 |
T10017 |
T10016 |
nsubj |
loss,leads |
R2674 |
T10018 |
T10017 |
prep |
of,loss |
R2675 |
T10019 |
T10018 |
pobj |
p150CAF,of |
R2676 |
T9864 |
T9862 |
ccomp |
altered,showed |
R2677 |
T10020 |
T10019 |
punct |
-,p150CAF |
R2678 |
T10021 |
T10019 |
nummod |
1,p150CAF |
R2679 |
T10022 |
T10016 |
prep |
to,leads |
R2680 |
T10023 |
T10022 |
pobj |
disruption,to |
R2681 |
T9865 |
T9866 |
det |
the,localization |
R2682 |
T10024 |
T10023 |
prep |
of,disruption |
R2683 |
T10025 |
T10026 |
compound |
heterochromatin,organization |
R2684 |
T10026 |
T10024 |
pobj |
organization,of |
R2685 |
T9866 |
T9864 |
nsubjpass |
localization,altered |
R2686 |
T10027 |
T10016 |
advmod |
independently,leads |
R2687 |
T10028 |
T10027 |
prep |
of,independently |
R2688 |
T10029 |
T10030 |
det |
the,degree |
R2689 |
T9867 |
T9866 |
amod |
nuclear,localization |
R2690 |
T10030 |
T10028 |
pobj |
degree,of |
R2691 |
T10031 |
T10030 |
prep |
of,degree |
R2692 |
T10032 |
T10033 |
compound |
DNA,methylation |
R2693 |
T10033 |
T10031 |
pobj |
methylation,of |
R2694 |
T10034 |
T10030 |
prep |
at,degree |
R2695 |
T9868 |
T9866 |
prep |
of,localization |
R2696 |
T10035 |
T10036 |
amod |
pericentric,repeats |
R2697 |
T10036 |
T10034 |
pobj |
repeats,at |
R2698 |
T10037 |
T10038 |
punct |
(,data |
R2699 |
T10038 |
T10014 |
meta |
data,confirmed |
R2700 |
T10039 |
T10038 |
amod |
unpublished,data |
R2701 |
T10040 |
T10038 |
punct |
),data |
R2702 |
T10041 |
T9975 |
punct |
.,tested |
R2703 |
T10043 |
T10044 |
nsubj |
We,analyzed |
R2704 |
T9869 |
T9868 |
pobj |
HP1α,of |
R2705 |
T10045 |
T10044 |
advmod |
next,analyzed |
R2706 |
T10046 |
T10047 |
amod |
specific,modifications |
R2707 |
T10047 |
T10044 |
dobj |
modifications,analyzed |
R2708 |
T10048 |
T10047 |
compound |
histone,modifications |
R2709 |
T10049 |
T10050 |
punct |
[,27 |
R2710 |
T9870 |
T9864 |
auxpass |
is,altered |
R2711 |
T10050 |
T10047 |
parataxis |
27,modifications |
R2712 |
T10051 |
T10050 |
punct |
],27 |
R2713 |
T10052 |
T10044 |
prep |
in,analyzed |
R2714 |
T9871 |
T9864 |
advmod |
severely,altered |
R2715 |
T10053 |
T10054 |
nmod |
chromatin,immunoprecipitation |
R2716 |
T10054 |
T10055 |
nmod |
immunoprecipitation,experiments |
R2717 |
T10055 |
T10052 |
pobj |
experiments,in |
R2718 |
T9872 |
T9864 |
prep |
in,altered |
R2719 |
T10056 |
T10054 |
punct |
(,immunoprecipitation |
R2720 |
T10057 |
T10054 |
appos |
ChIP,immunoprecipitation |
R2721 |
T10058 |
T10055 |
punct |
),experiments |
R2722 |
T10059 |
T10055 |
acl |
using,experiments |
R2723 |
T9873 |
T9874 |
nmod |
Chaf1a,embryos |
R2724 |
T10060 |
T10061 |
amod |
native,chromatin |
R2725 |
T10061 |
T10059 |
dobj |
chromatin,using |
R2726 |
T9874 |
T9872 |
pobj |
embryos,in |
R2727 |
T10062 |
T10063 |
punct |
[,28 |
R2728 |
T10063 |
T10044 |
parataxis |
28,analyzed |
R2729 |
T9875 |
T9873 |
punct |
−,Chaf1a |
R2730 |
T10064 |
T10063 |
punct |
],28 |
R2731 |
T9876 |
T9873 |
punct |
/,Chaf1a |
R2732 |
T10065 |
T10044 |
punct |
.,analyzed |
R2733 |
T10067 |
T10068 |
nsubj |
We,found |
R2734 |
T9877 |
T9873 |
punct |
−,Chaf1a |
R2735 |
T10069 |
T10070 |
mark |
that,immunoprecipitated |
R2736 |
T10070 |
T10068 |
ccomp |
immunoprecipitated,found |
R2737 |
T9878 |
T9874 |
cc |
and,embryos |
R2738 |
T10071 |
T10072 |
amod |
pericentric,DNA |
R2739 |
T10072 |
T10070 |
nsubjpass |
DNA,immunoprecipitated |
R2740 |
T10073 |
T10070 |
auxpass |
was,immunoprecipitated |
R2741 |
T10074 |
T10070 |
prep |
with,immunoprecipitated |
R2742 |
T9879 |
T9880 |
npadvmod |
p150CAF,depleted |
R2743 |
T10075 |
T10076 |
det |
a,efficiency |
R2744 |
T10076 |
T10074 |
pobj |
efficiency,with |
R2745 |
T9880 |
T9884 |
amod |
depleted,cells |
R2746 |
T10077 |
T10078 |
npadvmod |
2,lower |
R2747 |
T10078 |
T10076 |
amod |
lower,efficiency |
R2748 |
T10079 |
T10077 |
punct |
-,2 |
R2749 |
T10080 |
T10077 |
advmod |
fold,2 |
R2750 |
T9881 |
T9879 |
punct |
-,p150CAF |
R2751 |
T10081 |
T10070 |
prep |
with,immunoprecipitated |
R2752 |
T10082 |
T10083 |
nmod |
H4K20me3,antibodies |
R2753 |
T10083 |
T10081 |
pobj |
antibodies,with |
R2754 |
T10084 |
T10085 |
punct |
[,30 |
R2755 |
T10085 |
T10082 |
parataxis |
30,H4K20me3 |
R2756 |
T10086 |
T10085 |
nummod |
29,30 |
R2757 |
T10087 |
T10085 |
punct |
",",30 |
R2758 |
T9882 |
T9879 |
nummod |
1,p150CAF |
R2759 |
T10088 |
T10085 |
punct |
],30 |
R2760 |
T10089 |
T10070 |
prep |
following,immunoprecipitated |
R2761 |
T10090 |
T10091 |
nmod |
p150CAF,depletion |
R2762 |
T9883 |
T9880 |
punct |
-,depleted |
R2763 |
T10091 |
T10089 |
pobj |
depletion,following |
R2764 |
T10092 |
T10090 |
punct |
-,p150CAF |
R2765 |
T10093 |
T10090 |
nummod |
1,p150CAF |
R2766 |
T9884 |
T9874 |
conj |
cells,embryos |
R2767 |
T10094 |
T10095 |
punct |
(,7A |
R2768 |
T10095 |
T10068 |
parataxis |
7A,found |
R2769 |
T10096 |
T10095 |
compound |
Figure,7A |
R2770 |
T10097 |
T10095 |
cc |
and,7A |
R2771 |
T9885 |
T9884 |
compound |
ES,cells |
R2772 |
T10098 |
T10095 |
conj |
7B,7A |
R2773 |
T10099 |
T10095 |
punct |
),7A |
R2774 |
T10100 |
T10068 |
punct |
.,found |
R2775 |
T9886 |
T9862 |
punct |
.,showed |
R2776 |
T10102 |
T10103 |
nsubjpass |
Immunoprecipitation,affected |
R2777 |
T10104 |
T10102 |
prep |
of,Immunoprecipitation |
R2778 |
T9888 |
T9889 |
nsubj |
We,examined |
R2779 |
T10105 |
T10106 |
amod |
minor,repeats |
R2780 |
T10106 |
T10104 |
pobj |
repeats,of |
R2781 |
T10107 |
T10106 |
compound |
satellite,repeats |
R2782 |
T10108 |
T10102 |
prep |
in,Immunoprecipitation |
R2783 |
T10109 |
T10110 |
det |
the,experiment |
R2784 |
T9890 |
T9889 |
advmod |
therefore,examined |
R2785 |
T10110 |
T10108 |
pobj |
experiment,in |
R2786 |
T10111 |
T10110 |
amod |
same,experiment |
R2787 |
T10112 |
T10103 |
auxpass |
was,affected |
R2788 |
T10113 |
T10103 |
advmod |
less,affected |
R2789 |
T10114 |
T10115 |
punct |
(,7B |
R2790 |
T9891 |
T9892 |
det |
the,status |
R2791 |
T10115 |
T10103 |
parataxis |
7B,affected |
R2792 |
T10116 |
T10115 |
compound |
Figure,7B |
R2793 |
T10117 |
T10115 |
punct |
),7B |
R2794 |
T9892 |
T9889 |
dobj |
status,examined |
R2795 |
T10118 |
T10103 |
punct |
", ",affected |
R2796 |
T10119 |
T10103 |
advcl |
showing,affected |
R2797 |
T10120 |
T10121 |
mark |
that,affects |
R2798 |
T9893 |
T9892 |
prep |
of,status |
R2799 |
T10121 |
T10119 |
ccomp |
affects,showing |
R2800 |
T9894 |
T9895 |
amod |
other,marks |
R2801 |
T9895 |
T9893 |
pobj |
marks,of |
R2802 |
T9896 |
T9895 |
amod |
epigenetic,marks |
R2803 |
T10122 |
T10121 |
nsubj |
loss,affects |
R2804 |
T10123 |
T10122 |
prep |
of,loss |
R2805 |
T10124 |
T10125 |
nmod |
p150CAF,function |
R2806 |
T10125 |
T10123 |
pobj |
function,of |
R2807 |
T10126 |
T10124 |
punct |
-,p150CAF |
R2808 |
T9897 |
T9898 |
advmod |
previously,shown |
R2809 |
T10127 |
T10124 |
nummod |
1,p150CAF |
R2810 |
T10128 |
T10121 |
advmod |
mainly,affects |
R2811 |
T10129 |
T10121 |
dobj |
H4K20me3,affects |
R2812 |
T10130 |
T10129 |
prep |
at,H4K20me3 |
R2813 |
T9898 |
T9895 |
acl |
shown,marks |
R2814 |
T10131 |
T10132 |
amod |
pericentric,heterochromatin |
R2815 |
T9899 |
T9900 |
aux |
to,characterize |
R2816 |
T10132 |
T10130 |
pobj |
heterochromatin,at |
R2817 |
T9900 |
T9898 |
xcomp |
characterize,shown |
R2818 |
T10133 |
T10103 |
punct |
.,affected |
R2819 |
T9901 |
T9902 |
amod |
pericentric,heterochromatin |
R2820 |
T10135 |
T10136 |
advmod |
Moreover,detected |
R2821 |
T9902 |
T9900 |
dobj |
heterochromatin,characterize |
R2822 |
T10137 |
T10136 |
punct |
", ",detected |
R2823 |
T10138 |
T10139 |
det |
no,difference |
R2824 |
T9903 |
T9889 |
punct |
.,examined |
R2825 |
T10139 |
T10136 |
nsubjpass |
difference,detected |
R2826 |
T10140 |
T10139 |
amod |
significant,difference |
R2827 |
T10141 |
T10139 |
prep |
in,difference |
R2828 |
T9905 |
T9906 |
nsubj |
We,tested |
R2829 |
T10142 |
T10143 |
compound |
immunoprecipitation,efficiency |
R2830 |
T10143 |
T10141 |
pobj |
efficiency,in |
R2831 |
T10144 |
T10136 |
auxpass |
was,detected |
R2832 |
T10145 |
T10136 |
prep |
at,detected |
R2833 |
T9907 |
T9906 |
advmod |
first,tested |
R2834 |
T10146 |
T10147 |
amod |
intracisternal,particle |
R2835 |
T10147 |
T10149 |
nmod |
particle,elements |
R2836 |
T10148 |
T10147 |
nmod |
A,particle |
R2837 |
T9908 |
T9909 |
compound |
DNA,methylation |
R2838 |
T10149 |
T10145 |
pobj |
elements,at |
R2839 |
T10150 |
T10147 |
punct |
(,particle |
R2840 |
T9909 |
T9906 |
dobj |
methylation,tested |
R2841 |
T10151 |
T10147 |
appos |
IAP,particle |
R2842 |
T10152 |
T10149 |
punct |
),elements |
R2843 |
T10153 |
T10154 |
punct |
(,7B |
R2844 |
T9910 |
T9909 |
compound |
CpG,methylation |
R2845 |
T10154 |
T10149 |
parataxis |
7B,elements |
R2846 |
T10155 |
T10154 |
compound |
Figure,7B |
R2847 |
T10156 |
T10154 |
punct |
),7B |
R2848 |
T9911 |
T9912 |
punct |
[,25 |
R2849 |
T10157 |
T10149 |
punct |
", ",elements |
R2850 |
T10158 |
T10159 |
dep |
which,are |
R2851 |
T10159 |
T10149 |
relcl |
are,elements |
R2852 |
T10227 |
T10224 |
conj |
7B,7A |
R2853 |
T10160 |
T10161 |
amod |
noncentromeric,elements |
R2854 |
T10161 |
T10159 |
attr |
elements,are |
R2855 |
T10162 |
T10161 |
amod |
repeated,elements |
R2856 |
T10163 |
T10161 |
compound |
DNA,elements |
R2857 |
T10164 |
T10165 |
advmod |
also,enriched |
R2858 |
T10165 |
T10161 |
acl |
enriched,elements |
R2859 |
T10166 |
T10165 |
prep |
in,enriched |
R2860 |
T10167 |
T10166 |
pobj |
H4K20me3,in |
R2861 |
T10168 |
T10169 |
punct |
[,31 |
R2862 |
T10228 |
T10224 |
punct |
),7A |
R2863 |
T10169 |
T10136 |
parataxis |
31,detected |
R2864 |
T10170 |
T10169 |
punct |
],31 |
R2865 |
T10171 |
T10136 |
punct |
.,detected |
R2866 |
T10229 |
T10201 |
punct |
.,reduced |
R2867 |
T10173 |
T10174 |
det |
This,result |
R2868 |
T10174 |
T10175 |
nsubj |
result,shows |
R2869 |
T10231 |
T10232 |
prep |
In,are |
R2870 |
T10176 |
T10177 |
mark |
that,contributes |
R2871 |
T10177 |
T10175 |
ccomp |
contributes,shows |
R2872 |
T10178 |
T10177 |
nsubj |
p150CAF,contributes |
R2873 |
T10179 |
T10178 |
punct |
-,p150CAF |
R2874 |
T10233 |
T10234 |
amod |
wild,type |
R2875 |
T10180 |
T10178 |
nummod |
1,p150CAF |
R2876 |
T10181 |
T10177 |
prep |
to,contributes |
R2877 |
T10182 |
T10183 |
amod |
normal,levels |
R2878 |
T10183 |
T10181 |
pobj |
levels,to |
R2879 |
T10234 |
T10236 |
compound |
type,cells |
R2880 |
T10184 |
T10183 |
prep |
of,levels |
R2881 |
T10185 |
T10184 |
pobj |
H4K20me3,of |
R2882 |
T10186 |
T10177 |
prep |
at,contributes |
R2883 |
T10235 |
T10234 |
punct |
-,type |
R2884 |
T10187 |
T10188 |
amod |
pericentric,heterochromatin |
R2885 |
T10188 |
T10186 |
pobj |
heterochromatin,at |
R2886 |
T10189 |
T10177 |
punct |
", ",contributes |
R2887 |
T10190 |
T10177 |
cc |
but,contributes |
R2888 |
T10236 |
T10231 |
pobj |
cells,In |
R2889 |
T10191 |
T10192 |
neg |
not,necessarily |
R2890 |
T10192 |
T10177 |
conj |
necessarily,contributes |
R2891 |
T10237 |
T10232 |
punct |
", ",are |
R2892 |
T10193 |
T10192 |
prep |
at,necessarily |
R2893 |
T10194 |
T10195 |
amod |
other,loci |
R2894 |
T10195 |
T10193 |
pobj |
loci,at |
R2895 |
T10238 |
T10232 |
nsubj |
most,are |
R2896 |
T10196 |
T10175 |
punct |
.,shows |
R2897 |
T10198 |
T10199 |
det |
The,mark |
R2898 |
T10199 |
T10201 |
nsubjpass |
mark,reduced |
R2899 |
T10200 |
T10199 |
compound |
H3K9me3,mark |
R2900 |
T10239 |
T10238 |
prep |
of,most |
R2901 |
T10202 |
T10203 |
punct |
[,32 |
R2902 |
T10203 |
T10199 |
parataxis |
32,mark |
R2903 |
T10240 |
T10241 |
nmod |
H4K20me3,signals |
R2904 |
T10204 |
T10203 |
punct |
],32 |
R2905 |
T10205 |
T10201 |
auxpass |
was,reduced |
R2906 |
T10206 |
T10201 |
advmod |
also,reduced |
R2907 |
T10241 |
T10239 |
pobj |
signals,of |
R2908 |
T10207 |
T10201 |
advmod |
significantly,reduced |
R2909 |
T10208 |
T10201 |
prep |
in,reduced |
R2910 |
T10209 |
T10210 |
npadvmod |
p150CAF,depleted |
R2911 |
T10210 |
T10214 |
amod |
depleted,cells |
R2912 |
T10211 |
T10209 |
punct |
-,p150CAF |
R2913 |
T10212 |
T10209 |
nummod |
1,p150CAF |
R2914 |
T10213 |
T10210 |
punct |
-,depleted |
R2915 |
T10242 |
T10240 |
cc |
and,H4K20me3 |
R2916 |
T10214 |
T10208 |
pobj |
cells,in |
R2917 |
T10215 |
T10201 |
punct |
", ",reduced |
R2918 |
T10216 |
T10217 |
mark |
though,to |
R2919 |
T10217 |
T10201 |
advcl |
to,reduced |
R2920 |
T10243 |
T10240 |
conj |
H3K9me3,H4K20me3 |
R2921 |
T10218 |
T10219 |
det |
a,extent |
R2922 |
T10219 |
T10217 |
pobj |
extent,to |
R2923 |
T10220 |
T10219 |
amod |
lesser,extent |
R2924 |
T10244 |
T10232 |
acomp |
present,are |
R2925 |
T10221 |
T10219 |
prep |
than,extent |
R2926 |
T10222 |
T10221 |
pobj |
H4K20me3,than |
R2927 |
T10223 |
T10224 |
punct |
(,7A |
R2928 |
T10245 |
T10232 |
prep |
at,are |
R2929 |
T10224 |
T10201 |
parataxis |
7A,reduced |
R2930 |
T10225 |
T10224 |
compound |
Figure,7A |
R2931 |
T10226 |
T10224 |
cc |
and,7A |
R2932 |
T10246 |
T10247 |
det |
the,level |
R2933 |
T10247 |
T10245 |
pobj |
level,at |
R2934 |
T10248 |
T10247 |
prep |
of,level |
R2935 |
T10249 |
T10250 |
npadvmod |
DAPI,dense |
R2937 |
T10250 |
T10252 |
amod |
dense,domains |
R2939 |
T10251 |
T10250 |
punct |
-,dense |
R2943 |
T10252 |
T10248 |
pobj |
domains,of |
R2946 |
T10253 |
T10254 |
advmod |
when,visualized |
R2950 |
T10254 |
T10232 |
advcl |
visualized,are |
R2955 |
T10255 |
T10254 |
prep |
by,visualized |
R2958 |
T10256 |
T10255 |
pobj |
IF,by |
R2965 |
T10257 |
T10258 |
punct |
[,29 |
R2969 |
T10258 |
T10254 |
parataxis |
29,visualized |
R2972 |
T10361 |
T10362 |
compound |
ES,cells |
R2973 |
T10362 |
T10360 |
pobj |
cells,in |
R2974 |
T10259 |
T10258 |
punct |
],29 |
R2975 |
T10363 |
T10354 |
prep |
to,leads |
R2976 |
T10364 |
T10365 |
det |
a,disruption |
R2977 |
T10365 |
T10363 |
pobj |
disruption,to |
R2978 |
T10260 |
T10261 |
punct |
(,7C |
R2979 |
T10366 |
T10365 |
amod |
simultaneous,disruption |
R2980 |
T10367 |
T10365 |
prep |
of,disruption |
R2981 |
T10368 |
T10369 |
nmod |
heterochromatin,organization |
R2982 |
T10261 |
T10232 |
parataxis |
7C,are |
R2983 |
T10369 |
T10367 |
pobj |
organization,of |
R2984 |
T10370 |
T10371 |
nummod |
3,D |
R2985 |
T10371 |
T10369 |
compound |
D,organization |
R2986 |
T10262 |
T10261 |
compound |
Figures,7C |
R2987 |
T10372 |
T10371 |
punct |
-,D |
R2988 |
T10373 |
T10365 |
cc |
and,disruption |
R2989 |
T10374 |
T10375 |
det |
an,alteration |
R2990 |
T10375 |
T10365 |
conj |
alteration,disruption |
R2991 |
T10263 |
T10261 |
cc |
and,7C |
R2992 |
T10376 |
T10375 |
prep |
in,alteration |
R2993 |
T10377 |
T10378 |
amod |
epigenetic,marking |
R2994 |
T10264 |
T10261 |
conj |
7D,7C |
R2995 |
T10378 |
T10376 |
pobj |
marking,in |
R2996 |
T10379 |
T10349 |
punct |
.,show |
R2997 |
T10265 |
T10261 |
punct |
),7C |
R2998 |
T10266 |
T10232 |
punct |
.,are |
R2999 |
T10268 |
T10269 |
nsubjpass |
H3K9me3,perturbed |
R3000 |
T10270 |
T10269 |
auxpass |
was,perturbed |
R3001 |
T10271 |
T10269 |
advmod |
severely,perturbed |
R3002 |
T10272 |
T10269 |
prep |
in,perturbed |
R3003 |
T10273 |
T10274 |
npadvmod |
p150CAF,depleted |
R3004 |
T10274 |
T10278 |
amod |
depleted,cells |
R3005 |
T10275 |
T10273 |
punct |
-,p150CAF |
R3006 |
T10276 |
T10273 |
nummod |
1,p150CAF |
R3007 |
T10277 |
T10274 |
punct |
-,depleted |
R3008 |
T10278 |
T10272 |
pobj |
cells,in |
R3009 |
T10279 |
T10269 |
punct |
", ",perturbed |
R3010 |
T10280 |
T10269 |
advcl |
displaying,perturbed |
R3011 |
T10281 |
T10282 |
det |
a,pattern |
R3012 |
T10282 |
T10280 |
dobj |
pattern,displaying |
R3013 |
T10283 |
T10282 |
amod |
diffuse,pattern |
R3014 |
T10284 |
T10282 |
amod |
similar,pattern |
R3015 |
T10285 |
T10284 |
prep |
to,similar |
R3016 |
T10286 |
T10285 |
pobj |
HP1α,to |
R3017 |
T10287 |
T10288 |
punct |
(,7C |
R3018 |
T10288 |
T10269 |
parataxis |
7C,perturbed |
R3019 |
T10289 |
T10288 |
compound |
Figure,7C |
R3020 |
T10290 |
T10288 |
punct |
),7C |
R3021 |
T10291 |
T10269 |
punct |
.,perturbed |
R3022 |
T10293 |
T10294 |
det |
The,pattern |
R3023 |
T10294 |
T10296 |
nsubjpass |
pattern,altered |
R3024 |
T10295 |
T10294 |
compound |
H4K20me3,pattern |
R3025 |
T10297 |
T10296 |
auxpass |
was,altered |
R3026 |
T10298 |
T10299 |
advmod |
even,more |
R3027 |
T10299 |
T10300 |
advmod |
more,severely |
R3028 |
T10300 |
T10296 |
advmod |
severely,altered |
R3029 |
T10301 |
T10296 |
punct |
", ",altered |
R3030 |
T10302 |
T10296 |
advcl |
showing,altered |
R3031 |
T10303 |
T10304 |
preconj |
both,loss |
R3032 |
T10304 |
T10302 |
dobj |
loss,showing |
R3033 |
T10305 |
T10304 |
det |
a,loss |
R3034 |
T10306 |
T10304 |
prep |
of,loss |
R3035 |
T10307 |
T10308 |
det |
the,dots |
R3036 |
T10308 |
T10306 |
pobj |
dots,of |
R3037 |
T10309 |
T10308 |
amod |
typical,dots |
R3038 |
T10310 |
T10308 |
acl |
revealed,dots |
R3039 |
T10311 |
T10310 |
prep |
in,revealed |
R3040 |
T10312 |
T10313 |
compound |
control,cells |
R3041 |
T10313 |
T10311 |
pobj |
cells,in |
R3042 |
T10314 |
T10304 |
cc |
and,loss |
R3043 |
T10315 |
T10316 |
det |
a,reduction |
R3044 |
T10316 |
T10304 |
conj |
reduction,loss |
R3045 |
T10317 |
T10316 |
prep |
in,reduction |
R3046 |
T10318 |
T10319 |
compound |
signal,intensity |
R3047 |
T10319 |
T10317 |
pobj |
intensity,in |
R3048 |
T10320 |
T10321 |
punct |
(,7D |
R3049 |
T10321 |
T10296 |
parataxis |
7D,altered |
R3050 |
T10322 |
T10321 |
compound |
Figure,7D |
R3051 |
T10323 |
T10321 |
punct |
),7D |
R3052 |
T10324 |
T10296 |
punct |
.,altered |
R3053 |
T10326 |
T10327 |
advcl |
Consistent,detected |
R3054 |
T10328 |
T10326 |
prep |
with,Consistent |
R3055 |
T10329 |
T10330 |
poss |
our,experiments |
R3056 |
T10330 |
T10328 |
pobj |
experiments,with |
R3057 |
T10331 |
T10330 |
compound |
ChIP,experiments |
R2936 |
T10332 |
T10327 |
punct |
", ",detected |
R2938 |
T10333 |
T10334 |
amod |
residual,epitopes |
R2940 |
T10334 |
T10327 |
nsubjpass |
epitopes,detected |
R2941 |
T10335 |
T10334 |
compound |
H4K20me3,epitopes |
R2942 |
T10336 |
T10327 |
auxpass |
were,detected |
R2944 |
T10337 |
T10327 |
prep |
at,detected |
R2945 |
T10338 |
T10339 |
det |
the,level |
R2947 |
T10339 |
T10337 |
pobj |
level,at |
R2948 |
T10340 |
T10339 |
prep |
of,level |
R2949 |
T10341 |
T10342 |
amod |
relocated,material |
R2951 |
T10342 |
T10340 |
pobj |
material,of |
R2952 |
T10343 |
T10344 |
npadvmod |
DAPI,dense |
R2953 |
T10344 |
T10342 |
amod |
dense,material |
R2954 |
T10345 |
T10344 |
punct |
-,dense |
R2956 |
T10346 |
T10327 |
punct |
.,detected |
R2957 |
T10348 |
T10349 |
advmod |
Altogether,show |
R2959 |
T10350 |
T10349 |
punct |
", ",show |
R2960 |
T10351 |
T10352 |
poss |
our,results |
R2961 |
T10352 |
T10349 |
nsubj |
results,show |
R2962 |
T10353 |
T10354 |
mark |
that,leads |
R2963 |
T10354 |
T10349 |
ccomp |
leads,show |
R2964 |
T10355 |
T10354 |
nsubj |
depletion,leads |
R2966 |
T10356 |
T10355 |
prep |
of,depletion |
R2967 |
T10357 |
T10356 |
pobj |
CAF,of |
R2968 |
T10358 |
T10357 |
punct |
-,CAF |
R2970 |
T10359 |
T10357 |
nummod |
1,CAF |
R2971 |
T10360 |
T10355 |
prep |
in,depletion |