PMC:1630711 / 5821-27395 JSON TXT

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Id	Subject	Object	Predicate	Lexical cue
T7971	8-10	PRP	denotes	We
T7972	11-15	VBD	denotes	used
T7973	16-20	NN	denotes	gene
T7974	21-30	NN	denotes	targeting
T7975	31-33	IN	denotes	in
T7976	34-36	NN	denotes	ES
T7977	37-42	NNS	denotes	cells
T7978	43-45	TO	denotes	to
T7979	46-52	VB	denotes	delete
T7980	53-57	NN	denotes	exon
T7981	58-59	CD	denotes	3
T7982	60-62	IN	denotes	in
T7983	63-66	DT	denotes	the
T7984	74-78	NN	denotes	gene
T7985	67-73	NN	denotes	Chaf1a
T7986	78-80	,	denotes	,
T7987	80-85	WDT	denotes	which
T7988	86-93	VBZ	denotes	encodes
T7989	94-101	NN	denotes	p150CAF
T7990	101-102	HYPH	denotes	-
T7991	102-103	CD	denotes	1
T7992	104-105	-LRB-	denotes	(
T7993	112-114	NN	denotes	1A
T7994	105-111	NN	denotes	Figure
T7995	114-115	-RRB-	denotes	)
T7996	115-116	.	denotes	.
T7997	116-254	sentence	denotes	Chaf1a +/− mice were born at a Mendelian frequency, were of normal size and weight, displayed no obvious abnormalities, and were fertile.
T7998	117-123	NN	denotes	Chaf1a
T7999	128-132	NNS	denotes	mice
T8000	124-125	SYM	denotes	+
T8001	125-126	HYPH	denotes	/
T8002	126-127	SYM	denotes	−
T8003	138-142	VBN	denotes	born
T8004	133-137	VBD	denotes	were
T8005	143-145	IN	denotes	at
T8006	146-147	DT	denotes	a
T8007	158-167	NN	denotes	frequency
T8008	148-157	JJ	denotes	Mendelian
T8009	167-169	,	denotes	,
T8010	169-173	VBD	denotes	were
T8011	174-176	IN	denotes	of
T8012	177-183	JJ	denotes	normal
T8013	184-188	NN	denotes	size
T8014	189-192	CC	denotes	and
T8015	193-199	NN	denotes	weight
T8016	199-201	,	denotes	,
T8017	201-210	VBD	denotes	displayed
T8018	211-213	DT	denotes	no
T8019	222-235	NNS	denotes	abnormalities
T8020	214-221	JJ	denotes	obvious
T8021	235-237	,	denotes	,
T8022	237-240	CC	denotes	and
T8023	241-245	VBD	denotes	were
T8024	246-253	JJ	denotes	fertile
T8025	253-254	.	denotes	.
T8026	254-332	sentence	denotes	Crossing heterozygous mice failed to generate viable newborn Chaf1a −/− mice.
T8027	255-263	VBG	denotes	Crossing
T8028	282-288	VBD	denotes	failed
T8029	264-276	JJ	denotes	heterozygous
T8030	277-281	NNS	denotes	mice
T8031	289-291	TO	denotes	to
T8032	292-300	VB	denotes	generate
T8033	301-307	JJ	denotes	viable
T8034	327-331	NNS	denotes	mice
T8035	308-315	JJ	denotes	newborn
T8036	316-322	NN	denotes	Chaf1a
T8037	323-324	SYM	denotes	−
T8038	324-325	HYPH	denotes	/
T8039	325-326	SYM	denotes	−
T8040	331-332	.	denotes	.
T8041	332-432	sentence	denotes	Furthermore, no homozygous Chaf1a −/− embryos were detected at any of the post-implantation stages.
T8042	333-344	RB	denotes	Furthermore
T8043	384-392	VBN	denotes	detected
T8044	344-346	,	denotes	,
T8045	346-348	DT	denotes	no
T8046	371-378	NNS	denotes	embryos
T8047	349-359	JJ	denotes	homozygous
T8048	360-366	NN	denotes	Chaf1a
T8049	367-368	SYM	denotes	−
T8050	368-369	HYPH	denotes	/
T8051	369-370	SYM	denotes	−
T8052	379-383	VBD	denotes	were
T8053	393-395	IN	denotes	at
T8054	396-399	DT	denotes	any
T8055	400-402	IN	denotes	of
T8056	403-406	DT	denotes	the
T8057	425-431	NNS	denotes	stages
T8058	407-424	JJ	denotes	post-implantation
T8059	431-432	.	denotes	.
T8060	432-534	sentence	denotes	However, we could detect Chaf1a −/− embryos at embryonic day 4 (E4) using a PCR strategy (Figure 1B).
T8061	433-440	RB	denotes	However
T8062	451-457	VB	denotes	detect
T8063	440-442	,	denotes	,
T8064	442-444	PRP	denotes	we
T8065	445-450	MD	denotes	could
T8066	458-464	NN	denotes	Chaf1a
T8067	469-476	NNS	denotes	embryos
T8068	465-466	SYM	denotes	−
T8069	466-467	HYPH	denotes	/
T8070	467-468	SYM	denotes	−
T8071	477-479	IN	denotes	at
T8072	480-489	JJ	denotes	embryonic
T8073	490-493	NN	denotes	day
T8074	494-495	CD	denotes	4
T8075	496-497	-LRB-	denotes	(
T8076	497-499	NN	denotes	E4
T8077	499-500	-RRB-	denotes	)
T8078	501-506	VBG	denotes	using
T8079	507-508	DT	denotes	a
T8080	513-521	NN	denotes	strategy
T8081	509-512	NN	denotes	PCR
T8082	522-523	-LRB-	denotes	(
T8083	530-532	NN	denotes	1B
T8084	523-529	NN	denotes	Figure
T8085	532-533	-RRB-	denotes	)
T8086	533-534	.	denotes	.
T8087	534-715	sentence	denotes	They represent only 10% of E4 embryos obtained from Chaf1a +/− mice intercrosses, suggesting that more than half of the homozygous mutant embryos had degenerated before this stage.
T8088	535-539	PRP	denotes	They
T8089	540-549	VBP	denotes	represent
T8090	550-554	RB	denotes	only
T8091	555-557	CD	denotes	10
T8092	557-558	NN	denotes	%
T8093	559-561	IN	denotes	of
T8094	562-564	NN	denotes	E4
T8095	565-572	NNS	denotes	embryos
T8096	573-581	VBN	denotes	obtained
T8097	582-586	IN	denotes	from
T8098	587-593	NN	denotes	Chaf1a
T8099	603-615	NNS	denotes	intercrosses
T8100	594-595	SYM	denotes	+
T8101	595-596	HYPH	denotes	/
T8102	596-597	SYM	denotes	−
T8103	598-602	NNS	denotes	mice
T8104	615-617	,	denotes	,
T8105	617-627	VBG	denotes	suggesting
T8106	628-632	IN	denotes	that
T8107	685-696	VBN	denotes	degenerated
T8108	633-637	JJR	denotes	more
T8109	643-647	NN	denotes	half
T8110	638-642	IN	denotes	than
T8111	648-650	IN	denotes	of
T8112	651-654	DT	denotes	the
T8113	673-680	NNS	denotes	embryos
T8114	655-665	JJ	denotes	homozygous
T8115	666-672	NN	denotes	mutant
T8116	681-684	VBD	denotes	had
T8117	697-703	IN	denotes	before
T8118	704-708	DT	denotes	this
T8119	709-714	NN	denotes	stage
T8120	714-715	.	denotes	.
T8121	715-881	sentence	denotes	Moreover, mutant embryos contained only eight to 16 cells at the E4 stage, instead of 32 cells observed for wild-type or heterozygous blastocysts (Figure 1C and 1D).
T8122	716-724	RB	denotes	Moreover
T8123	741-750	VBD	denotes	contained
T8124	724-726	,	denotes	,
T8125	726-732	NN	denotes	mutant
T8126	733-740	NNS	denotes	embryos
T8127	751-755	RB	denotes	only
T8128	765-767	CD	denotes	16
T8129	756-761	CD	denotes	eight
T8130	762-764	IN	denotes	to
T8131	768-773	NNS	denotes	cells
T8132	774-776	IN	denotes	at
T8133	777-780	DT	denotes	the
T8134	784-789	NN	denotes	stage
T8135	781-783	NN	denotes	E4
T8136	789-791	,	denotes	,
T8137	791-798	RB	denotes	instead
T8138	799-801	IN	denotes	of
T8139	802-804	CD	denotes	32
T8140	805-810	NNS	denotes	cells
T8141	811-819	VBN	denotes	observed
T8142	820-823	IN	denotes	for
T8143	824-828	JJ	denotes	wild
T8144	829-833	NN	denotes	type
T8145	828-829	HYPH	denotes	-
T8146	850-861	NNS	denotes	blastocysts
T8147	834-836	CC	denotes	or
T8148	837-849	JJ	denotes	heterozygous
T8149	862-863	-LRB-	denotes	(
T8150	870-872	NN	denotes	1C
T8151	863-869	NN	denotes	Figure
T8152	873-876	CC	denotes	and
T8153	877-879	NN	denotes	1D
T8154	879-880	-RRB-	denotes	)
T8155	880-881	.	denotes	.
T8156	881-1012	sentence	denotes	Further analysis by light microscopy using immunofluorescence (IF) allowed us to compare wild-type and p150CAF-1-depleted embryos.
T8157	882-889	JJ	denotes	Further
T8158	890-898	NN	denotes	analysis
T8159	949-956	VBD	denotes	allowed
T8160	899-901	IN	denotes	by
T8161	902-907	NN	denotes	light
T8162	908-918	NN	denotes	microscopy
T8163	919-924	VBG	denotes	using
T8164	925-943	NN	denotes	immunofluorescence
T8165	944-945	-LRB-	denotes	(
T8166	945-947	NN	denotes	IF
T8167	947-948	-RRB-	denotes	)
T8168	957-959	PRP	denotes	us
T8169	963-970	VB	denotes	compare
T8170	960-962	TO	denotes	to
T8171	971-975	JJ	denotes	wild
T8172	976-980	NN	denotes	type
T8173	975-976	HYPH	denotes	-
T8174	1004-1011	NNS	denotes	embryos
T8175	981-984	CC	denotes	and
T8176	985-992	NN	denotes	p150CAF
T8177	995-1003	VBN	denotes	depleted
T8178	992-993	HYPH	denotes	-
T8179	993-994	CD	denotes	1
T8180	994-995	HYPH	denotes	-
T8181	1011-1012	.	denotes	.
T8182	1012-1209	sentence	denotes	A loss of p150CAF-1 staining could not be detected before the eight-cell stage (unpublished data) suggesting that maternally contributed protein is present in the embryo up to the four-cell stage.
T8183	1013-1014	DT	denotes	A
T8184	1015-1019	NN	denotes	loss
T8185	1055-1063	VBN	denotes	detected
T8186	1020-1022	IN	denotes	of
T8187	1023-1030	NN	denotes	p150CAF
T8188	1033-1041	NN	denotes	staining
T8189	1030-1031	HYPH	denotes	-
T8190	1031-1032	CD	denotes	1
T8191	1042-1047	MD	denotes	could
T8192	1048-1051	RB	denotes	not
T8193	1052-1054	VB	denotes	be
T8194	1064-1070	IN	denotes	before
T8195	1071-1074	DT	denotes	the
T8196	1086-1091	NN	denotes	stage
T8197	1075-1080	CD	denotes	eight
T8198	1081-1085	NN	denotes	cell
T8199	1080-1081	HYPH	denotes	-
T8200	1092-1093	-LRB-	denotes	(
T8201	1105-1109	NNS	denotes	data
T8202	1093-1104	JJ	denotes	unpublished
T8203	1109-1110	-RRB-	denotes	)
T8204	1111-1121	VBG	denotes	suggesting
T8205	1122-1126	IN	denotes	that
T8206	1158-1160	VBZ	denotes	is
T8207	1127-1137	RB	denotes	maternally
T8208	1138-1149	VBN	denotes	contributed
T8209	1150-1157	NN	denotes	protein
T8210	1161-1168	JJ	denotes	present
T8211	1169-1171	IN	denotes	in
T8212	1172-1175	DT	denotes	the
T8213	1176-1182	NN	denotes	embryo
T8214	1183-1185	IN	denotes	up
T8215	1186-1188	IN	denotes	to
T8216	1189-1192	DT	denotes	the
T8217	1203-1208	NN	denotes	stage
T8218	1193-1197	CD	denotes	four
T8219	1198-1202	NN	denotes	cell
T8220	1197-1198	HYPH	denotes	-
T8221	1208-1209	.	denotes	.
T8222	1209-1341	sentence	denotes	Thus, depletion of p150CAF-1 protein from this stage allows a maximum of two additional cell divisions before developmental arrest.
T8223	1210-1214	RB	denotes	Thus
T8224	1263-1269	VBZ	denotes	allows
T8225	1214-1216	,	denotes	,
T8226	1216-1225	NN	denotes	depletion
T8227	1226-1228	IN	denotes	of
T8228	1229-1236	NN	denotes	p150CAF
T8229	1239-1246	NN	denotes	protein
T8230	1236-1237	HYPH	denotes	-
T8231	1237-1238	CD	denotes	1
T8232	1247-1251	IN	denotes	from
T8233	1252-1256	DT	denotes	this
T8234	1257-1262	NN	denotes	stage
T8235	1270-1271	DT	denotes	a
T8236	1272-1279	NN	denotes	maximum
T8237	1280-1282	IN	denotes	of
T8238	1283-1286	CD	denotes	two
T8239	1303-1312	NNS	denotes	divisions
T8240	1287-1297	JJ	denotes	additional
T8241	1298-1302	NN	denotes	cell
T8242	1313-1319	IN	denotes	before
T8243	1320-1333	JJ	denotes	developmental
T8244	1334-1340	NN	denotes	arrest
T8245	1340-1341	.	denotes	.
T8246	1341-4028	sentence	denotes	Figure 1 Loss of p150CAF-1 Function Leads to Early Developmental Arrest and Alteration of Heterochromatin Organization (A) Generation of Chaf1a +/− mice by homologous recombination in ES cells. (Top) Scheme of the p150CAF1 protein indicating the interacting domains (ID) for HP1 and p60CAF-1, and the acidic domain (AD). (Middle) Structure of the murine Chaf1a gene. Blocks and lines represent exons and introns, respectively. A star indicates the translation initiation site. Below is shown the Chaf1a targeting vector, which includes genomic DNA homology regions (5 HR and 3 HR), the diphtheria toxin (DT), and neomycin selection genes. Recombined (Rec) ES cells and mice were identified by Southern blot using ScaI (S) and the indicated probe. (B) Breeding strategy used for the production of E4 Chaf1a −/− embryos. Genotyping was performed by PCR using the three oligonucleotide primers indicated in (A) (1, 2, and 3). An example of the result of a genotyping experiment is shown. (C) Immunodetection of p150CAF-1 (green) and HP1α (red) in E4 embryos derived from Chaf1a +/− intercrosses. Because Chaf1a +/+ and Chaf1a +/− embryos both stain positively for the presence of p150CAF-1, they are both designated as wild-type. p150CAF-1 expression, which indicates ongoing S phase, can be detected in most cells within the wild-type blastocyst (upper panel). The lower panel shows a 12-cell embryo labeling negatively for p150CAF-1. Only nonspecific background labeling can be observed. In each panel, the right-hand image shows the merge between the HP1α fluorescence and DAPI-stained DNA in blue. Pink color indicates the association of HP1 with DAPI-dense material. The bottom of each panel shows the magnification of a nucleus selected from the above embryo (white square). The arrowhead indicates the typical heterochromatin foci revealed by DAPI and HP1α staining in wild-type embryos. These foci are not visible in Chaf1a −/− embryos. DAPI and HP1α staining are diffuse within the nucleus of p150CAF-1-depleted embryos, with enrichment at the nuclear periphery, revealing abnormal heterochromatin organization. Scale bars represent 10 μm. (E) Wild-type embryos isolated at embryonic day 2 (E2, two cells), E2.5 (four cells), E3 (eight cells), E3.5 (16 cells), and E4 (32 cells) are shown. Heterochromatin was monitored by DAPI staining (upper panel) and HP1 immunolabeling (in red, lower panel). (F) Magnification of a nucleus (DAPI-stained) representative of each stage. The right-hand panel shows the nucleus of an ES cell. Scale bar represents 10 μm. Strikingly, we found that the nuclear organization of heterochromatin appeared abnormal in the nuclei of Chaf1a −/− embryos.
T8247	3904-3914	RB	denotes	Strikingly
T8248	3919-3924	VBD	denotes	found
T8249	3914-3916	,	denotes	,
T8250	3916-3918	PRP	denotes	we
T8251	3925-3929	IN	denotes	that
T8252	3974-3982	VBD	denotes	appeared
T8253	3930-3933	DT	denotes	the
T8254	3942-3954	NN	denotes	organization
T8255	3934-3941	JJ	denotes	nuclear
T8256	3955-3957	IN	denotes	of
T8257	3958-3973	NN	denotes	heterochromatin
T8258	3983-3991	JJ	denotes	abnormal
T8259	3992-3994	IN	denotes	in
T8260	3995-3998	DT	denotes	the
T8261	3999-4005	NNS	denotes	nuclei
T8262	4006-4008	IN	denotes	of
T8263	4009-4015	NN	denotes	Chaf1a
T8264	4020-4027	NNS	denotes	embryos
T8265	4016-4017	SYM	denotes	−
T8266	4017-4018	HYPH	denotes	/
T8267	4018-4019	SYM	denotes	−
T8268	4027-4028	.	denotes	.
T8269	4028-4225	sentence	denotes	Pericentric heterochromatin, the major component of constitutive heterochromatin, can easily be visualized in interphase nuclei by the fluorochrome DAPI and by immunostaining with HP1α [16,17–19].
T8270	4029-4040	JJ	denotes	Pericentric
T8271	4041-4056	NN	denotes	heterochromatin
T8272	4125-4135	VBN	denotes	visualized
T8273	4056-4058	,	denotes	,
T8274	4058-4061	DT	denotes	the
T8275	4068-4077	NN	denotes	component
T8276	4062-4067	JJ	denotes	major
T8277	4078-4080	IN	denotes	of
T8278	4081-4093	JJ	denotes	constitutive
T8279	4094-4109	NN	denotes	heterochromatin
T8280	4109-4111	,	denotes	,
T8281	4111-4114	MD	denotes	can
T8282	4115-4121	RB	denotes	easily
T8283	4122-4124	VB	denotes	be
T8284	4136-4138	IN	denotes	in
T8285	4139-4149	NN	denotes	interphase
T8286	4150-4156	NNS	denotes	nuclei
T8287	4157-4159	IN	denotes	by
T8288	4160-4163	DT	denotes	the
T8289	4177-4181	NN	denotes	DAPI
T8290	4164-4176	NN	denotes	fluorochrome
T8291	4182-4185	CC	denotes	and
T8292	4186-4188	IN	denotes	by
T8293	4189-4203	VBG	denotes	immunostaining
T8294	4204-4208	IN	denotes	with
T8295	4209-4213	NN	denotes	HP1α
T8296	4214-4215	-LRB-	denotes	[
T8297	4215-4217	CD	denotes	16
T8298	4217-4218	,	denotes	,
T8299	4218-4220	CD	denotes	17
T8300	4220-4221	SYM	denotes	–
T8301	4221-4223	CD	denotes	19
T8302	4223-4224	-RRB-	denotes	]
T8303	4224-4225	.	denotes	.
T8304	4225-4454	sentence	denotes	In wild-type blastocysts and in mouse somatic cells, pericentric heterochromatin domains cluster together and form higher-order chromatin structures called chromocenters [20], which are visualized as DAPI-dense foci (Figure 1C).
T8305	4226-4228	IN	denotes	In
T8306	4315-4322	VBP	denotes	cluster
T8307	4229-4233	JJ	denotes	wild
T8308	4234-4238	NN	denotes	type
T8309	4233-4234	HYPH	denotes	-
T8310	4239-4250	NNS	denotes	blastocysts
T8311	4251-4254	CC	denotes	and
T8312	4255-4257	IN	denotes	in
T8313	4258-4263	NN	denotes	mouse
T8314	4272-4277	NNS	denotes	cells
T8315	4264-4271	JJ	denotes	somatic
T8316	4277-4279	,	denotes	,
T8317	4279-4290	JJ	denotes	pericentric
T8318	4307-4314	NNS	denotes	domains
T8319	4291-4306	NN	denotes	heterochromatin
T8320	4323-4331	RB	denotes	together
T8321	4332-4335	CC	denotes	and
T8322	4336-4340	VBP	denotes	form
T8323	4341-4347	JJR	denotes	higher
T8324	4348-4353	NN	denotes	order
T8325	4347-4348	HYPH	denotes	-
T8326	4364-4374	NNS	denotes	structures
T8327	4354-4363	NN	denotes	chromatin
T8328	4375-4381	VBN	denotes	called
T8329	4382-4395	NNS	denotes	chromocenters
T8330	4396-4397	-LRB-	denotes	[
T8331	4397-4399	CD	denotes	20
T8332	4399-4400	-RRB-	denotes	]
T8333	4400-4402	,	denotes	,
T8334	4402-4407	WDT	denotes	which
T8335	4412-4422	VBN	denotes	visualized
T8336	4408-4411	VBP	denotes	are
T8337	4423-4425	IN	denotes	as
T8338	4426-4430	NN	denotes	DAPI
T8339	4431-4436	JJ	denotes	dense
T8340	4430-4431	HYPH	denotes	-
T8341	4437-4441	NNS	denotes	foci
T8342	4442-4443	-LRB-	denotes	(
T8343	4450-4452	NN	denotes	1C
T8344	4443-4449	NN	denotes	Figure
T8345	4452-4453	-RRB-	denotes	)
T8346	4453-4454	.	denotes	.
T8347	4454-4524	sentence	denotes	These structures were not detected in Chaf1a −/− embryos (Figure 1D).
T8348	4455-4460	DT	denotes	These
T8349	4461-4471	NNS	denotes	structures
T8350	4481-4489	VBN	denotes	detected
T8351	4472-4476	VBD	denotes	were
T8352	4477-4480	RB	denotes	not
T8353	4490-4492	IN	denotes	in
T8354	4493-4499	NN	denotes	Chaf1a
T8355	4504-4511	NNS	denotes	embryos
T8356	4500-4501	SYM	denotes	−
T8357	4501-4502	HYPH	denotes	/
T8358	4502-4503	SYM	denotes	−
T8359	4512-4513	-LRB-	denotes	(
T8360	4520-4522	NN	denotes	1D
T8361	4513-4519	NN	denotes	Figure
T8362	4522-4523	-RRB-	denotes	)
T8363	4523-4524	.	denotes	.
T8364	4524-4679	sentence	denotes	Since chromatin architecture and higher-order structures are important for genome function [21], we decided to characterize this phenotype in more detail.
T8365	4525-4530	IN	denotes	Since
T8366	4582-4585	VBP	denotes	are
T8367	4531-4540	NN	denotes	chromatin
T8368	4541-4553	NN	denotes	architecture
T8369	4554-4557	CC	denotes	and
T8370	4558-4564	JJR	denotes	higher
T8371	4565-4570	NN	denotes	order
T8372	4564-4565	HYPH	denotes	-
T8373	4571-4581	NNS	denotes	structures
T8374	4625-4632	VBD	denotes	decided
T8375	4586-4595	JJ	denotes	important
T8376	4596-4599	IN	denotes	for
T8377	4600-4606	NN	denotes	genome
T8378	4607-4615	NN	denotes	function
T8379	4616-4617	-LRB-	denotes	[
T8380	4617-4619	CD	denotes	21
T8381	4619-4620	-RRB-	denotes	]
T8382	4620-4622	,	denotes	,
T8383	4622-4624	PRP	denotes	we
T8384	4633-4635	TO	denotes	to
T8385	4636-4648	VB	denotes	characterize
T8386	4649-4653	DT	denotes	this
T8387	4654-4663	NN	denotes	phenotype
T8388	4664-4666	IN	denotes	in
T8389	4667-4671	JJR	denotes	more
T8390	4672-4678	NN	denotes	detail
T8391	4678-4679	.	denotes	.
T8392	4679-4796	sentence	denotes	For this purpose, we examined the status of heterochromatin organization between the two-cell and blastocyst stages.
T8393	4680-4683	IN	denotes	For
T8394	4701-4709	VBD	denotes	examined
T8395	4684-4688	DT	denotes	this
T8396	4689-4696	NN	denotes	purpose
T8397	4696-4698	,	denotes	,
T8398	4698-4700	PRP	denotes	we
T8399	4710-4713	DT	denotes	the
T8400	4714-4720	NN	denotes	status
T8401	4721-4723	IN	denotes	of
T8402	4724-4739	NN	denotes	heterochromatin
T8403	4740-4752	NN	denotes	organization
T8404	4753-4760	IN	denotes	between
T8405	4761-4764	DT	denotes	the
T8406	4789-4795	NNS	denotes	stages
T8407	4765-4768	CD	denotes	two
T8408	4769-4773	NN	denotes	cell
T8409	4768-4769	HYPH	denotes	-
T8410	4774-4777	CC	denotes	and
T8411	4778-4788	NN	denotes	blastocyst
T8412	4795-4796	.	denotes	.
T8413	4796-4955	sentence	denotes	In two-cell stage wild-type embryos, DAPI staining is diffuse and fibrillar, with regions of higher density around the nucleolar precursor bodies (Figure 1E).
T8414	4797-4799	IN	denotes	In
T8415	4848-4850	VBZ	denotes	is
T8416	4800-4803	CD	denotes	two
T8417	4804-4808	NN	denotes	cell
T8418	4803-4804	HYPH	denotes	-
T8419	4809-4814	NN	denotes	stage
T8420	4825-4832	NNS	denotes	embryos
T8421	4815-4819	JJ	denotes	wild
T8422	4820-4824	NN	denotes	type
T8423	4819-4820	HYPH	denotes	-
T8424	4832-4834	,	denotes	,
T8425	4834-4838	NN	denotes	DAPI
T8426	4839-4847	NN	denotes	staining
T8427	4851-4858	JJ	denotes	diffuse
T8428	4859-4862	CC	denotes	and
T8429	4863-4872	JJ	denotes	fibrillar
T8430	4872-4874	,	denotes	,
T8431	4874-4878	IN	denotes	with
T8432	4879-4886	NNS	denotes	regions
T8433	4887-4889	IN	denotes	of
T8434	4890-4896	JJR	denotes	higher
T8435	4897-4904	NN	denotes	density
T8436	4905-4911	IN	denotes	around
T8437	4912-4915	DT	denotes	the
T8438	4936-4942	NNS	denotes	bodies
T8439	4916-4925	JJ	denotes	nucleolar
T8440	4926-4935	NN	denotes	precursor
T8441	4943-4944	-LRB-	denotes	(
T8442	4951-4953	NN	denotes	1E
T8443	4944-4950	NN	denotes	Figure
T8444	4953-4954	-RRB-	denotes	)
T8445	4954-4955	.	denotes	.
T8446	4955-5093	sentence	denotes	Heterochromatin domains are progressively assembled into DAPI-dense foci between the four-cell and 32-cell blastocyst stages (Figure 1F).
T8447	4956-4971	NN	denotes	Heterochromatin
T8448	4972-4979	NNS	denotes	domains
T8449	4998-5007	VBN	denotes	assembled
T8450	4980-4983	VBP	denotes	are
T8451	4984-4997	RB	denotes	progressively
T8452	5008-5012	IN	denotes	into
T8453	5013-5017	NN	denotes	DAPI
T8454	5018-5023	JJ	denotes	dense
T8455	5017-5018	HYPH	denotes	-
T8456	5024-5028	NNS	denotes	foci
T8457	5029-5036	IN	denotes	between
T8458	5037-5040	DT	denotes	the
T8459	5074-5080	NNS	denotes	stages
T8460	5041-5045	CD	denotes	four
T8461	5046-5050	NN	denotes	cell
T8462	5045-5046	HYPH	denotes	-
T8463	5051-5054	CC	denotes	and
T8464	5055-5057	CD	denotes	32
T8465	5058-5062	NN	denotes	cell
T8466	5057-5058	HYPH	denotes	-
T8467	5063-5073	NN	denotes	blastocyst
T8468	5081-5082	-LRB-	denotes	(
T8469	5089-5091	NN	denotes	1F
T8470	5082-5088	NN	denotes	Figure
T8471	5091-5092	-RRB-	denotes	)
T8472	5092-5093	.	denotes	.
T8473	5093-5228	sentence	denotes	Localization of HP1α confirmed that the structures visualized by DAPI staining correspond to constitutive heterochromatin (Figure 1E).
T8474	5094-5106	NN	denotes	Localization
T8475	5115-5124	VBD	denotes	confirmed
T8476	5107-5109	IN	denotes	of
T8477	5110-5114	NN	denotes	HP1α
T8478	5125-5129	IN	denotes	that
T8479	5173-5183	VBP	denotes	correspond
T8480	5130-5133	DT	denotes	the
T8481	5134-5144	NNS	denotes	structures
T8482	5145-5155	VBN	denotes	visualized
T8483	5156-5158	IN	denotes	by
T8484	5159-5163	NN	denotes	DAPI
T8485	5164-5172	NN	denotes	staining
T8486	5184-5186	IN	denotes	to
T8487	5187-5199	JJ	denotes	constitutive
T8488	5200-5215	NN	denotes	heterochromatin
T8489	5216-5217	-LRB-	denotes	(
T8490	5224-5226	NN	denotes	1E
T8491	5217-5223	NN	denotes	Figure
T8492	5226-5227	-RRB-	denotes	)
T8493	5227-5228	.	denotes	.
T8494	5228-5384	sentence	denotes	Thus, the nuclear organization of heterochromatin is dramatically modified during pre-implantation development, between the two-cell and blastocyst stages.
T8495	5229-5233	RB	denotes	Thus
T8496	5295-5303	VBN	denotes	modified
T8497	5233-5235	,	denotes	,
T8498	5235-5238	DT	denotes	the
T8499	5247-5259	NN	denotes	organization
T8500	5239-5246	JJ	denotes	nuclear
T8501	5260-5262	IN	denotes	of
T8502	5263-5278	NN	denotes	heterochromatin
T8503	5279-5281	VBZ	denotes	is
T8504	5282-5294	RB	denotes	dramatically
T8505	5304-5310	IN	denotes	during
T8506	5311-5327	JJ	denotes	pre-implantation
T8507	5328-5339	NN	denotes	development
T8508	5339-5341	,	denotes	,
T8509	5341-5348	IN	denotes	between
T8510	5349-5352	DT	denotes	the
T8511	5377-5383	NNS	denotes	stages
T8512	5353-5356	CD	denotes	two
T8513	5357-5361	NN	denotes	cell
T8514	5356-5357	HYPH	denotes	-
T8515	5362-5365	CC	denotes	and
T8516	5366-5376	NN	denotes	blastocyst
T8517	5383-5384	.	denotes	.
T8518	5384-5519	sentence	denotes	In Chaf1a −/− E4 embryos, which are arrested between the eight- and 16-cell stage, DAPI-dense foci were barely detectable (Figure 1D).
T8519	5385-5387	IN	denotes	In
T8520	5484-5488	VBD	denotes	were
T8521	5388-5394	NN	denotes	Chaf1a
T8522	5402-5409	NNS	denotes	embryos
T8523	5395-5396	SYM	denotes	−
T8524	5396-5397	HYPH	denotes	/
T8525	5397-5398	SYM	denotes	−
T8526	5399-5401	NN	denotes	E4
T8527	5409-5411	,	denotes	,
T8528	5411-5416	WDT	denotes	which
T8529	5421-5429	VBN	denotes	arrested
T8530	5417-5420	VBP	denotes	are
T8531	5430-5437	IN	denotes	between
T8532	5438-5441	DT	denotes	the
T8533	5461-5466	NN	denotes	stage
T8534	5442-5447	CD	denotes	eight
T8535	5456-5460	NN	denotes	cell
T8536	5447-5448	HYPH	denotes	-
T8537	5449-5452	CC	denotes	and
T8539	5455-5456	HYPH	denotes	-
T8540	5466-5468	,	denotes	,
T8541	5468-5472	NN	denotes	DAPI
T8542	5473-5478	JJ	denotes	dense
T8543	5472-5473	HYPH	denotes	-
T8544	5479-5483	NNS	denotes	foci
T8545	5489-5495	RB	denotes	barely
T8546	5496-5506	JJ	denotes	detectable
T8547	5507-5508	-LRB-	denotes	(
T8548	5515-5517	NN	denotes	1D
T8549	5508-5514	NN	denotes	Figure
T8550	5517-5518	-RRB-	denotes	)
T8551	5518-5519	.	denotes	.
T8552	5519-5673	sentence	denotes	Instead, DAPI staining was diffuse within the nucleus, with regions of higher density around the nucleoli and at the periphery of the nuclei (Figure 1D).
T8553	5520-5527	RB	denotes	Instead
T8554	5543-5546	VBD	denotes	was
T8555	5527-5529	,	denotes	,
T8556	5529-5533	NN	denotes	DAPI
T8557	5534-5542	NN	denotes	staining
T8558	5547-5554	JJ	denotes	diffuse
T8559	5555-5561	IN	denotes	within
T8560	5562-5565	DT	denotes	the
T8561	5566-5573	NN	denotes	nucleus
T8562	5573-5575	,	denotes	,
T8563	5575-5579	IN	denotes	with
T8564	5580-5587	NNS	denotes	regions
T8565	5588-5590	IN	denotes	of
T8566	5591-5597	JJR	denotes	higher
T8567	5598-5605	NN	denotes	density
T8568	5606-5612	IN	denotes	around
T8569	5613-5616	DT	denotes	the
T8570	5617-5625	NNS	denotes	nucleoli
T8571	5626-5629	CC	denotes	and
T8572	5630-5632	IN	denotes	at
T8573	5633-5636	DT	denotes	the
T8574	5637-5646	NN	denotes	periphery
T8575	5647-5649	IN	denotes	of
T8576	5650-5653	DT	denotes	the
T8577	5654-5660	NNS	denotes	nuclei
T8578	5661-5662	-LRB-	denotes	(
T8579	5669-5671	NN	denotes	1D
T8580	5662-5668	NN	denotes	Figure
T8581	5671-5672	-RRB-	denotes	)
T8582	5672-5673	.	denotes	.
T8583	5673-5824	sentence	denotes	Localization of HP1α showed a diffuse pattern similar to the DAPI, with some enrichment at the nuclear periphery, and around the nucleoli (Figure 1D).
T8584	5674-5686	NN	denotes	Localization
T8585	5695-5701	VBD	denotes	showed
T8586	5687-5689	IN	denotes	of
T8587	5690-5694	NN	denotes	HP1α
T8588	5702-5703	DT	denotes	a
T8589	5712-5719	NN	denotes	pattern
T8590	5704-5711	JJ	denotes	diffuse
T8591	5720-5727	JJ	denotes	similar
T8592	5728-5730	IN	denotes	to
T8593	5731-5734	DT	denotes	the
T8594	5735-5739	NN	denotes	DAPI
T8595	5739-5741	,	denotes	,
T8596	5741-5745	IN	denotes	with
T8597	5746-5750	DT	denotes	some
T8598	5751-5761	NN	denotes	enrichment
T8599	5762-5764	IN	denotes	at
T8600	5765-5768	DT	denotes	the
T8601	5777-5786	NN	denotes	periphery
T8602	5769-5776	JJ	denotes	nuclear
T8603	5786-5788	,	denotes	,
T8604	5788-5791	CC	denotes	and
T8605	5792-5798	IN	denotes	around
T8606	5799-5802	DT	denotes	the
T8607	5803-5811	NNS	denotes	nucleoli
T8608	5812-5813	-LRB-	denotes	(
T8609	5820-5822	NN	denotes	1D
T8610	5813-5819	NN	denotes	Figure
T8611	5822-5823	-RRB-	denotes	)
T8612	5823-5824	.	denotes	.
T8613	5824-6022	sentence	denotes	This abnormal organization of heterochromatin in Chaf1a −/− embryos is reminiscent of the heterochromatin organization found in two- to four-cell stage wild-type embryos (compare Figure 1D and 1F).
T8614	5825-5829	DT	denotes	This
T8615	5839-5851	NN	denotes	organization
T8616	5830-5838	JJ	denotes	abnormal
T8617	5893-5895	VBZ	denotes	is
T8618	5852-5854	IN	denotes	of
T8619	5855-5870	NN	denotes	heterochromatin
T8620	5871-5873	IN	denotes	in
T8621	5874-5880	NN	denotes	Chaf1a
T8622	5885-5892	NNS	denotes	embryos
T8623	5881-5882	SYM	denotes	−
T8624	5882-5883	HYPH	denotes	/
T8625	5883-5884	SYM	denotes	−
T8626	5896-5907	JJ	denotes	reminiscent
T8627	5908-5910	IN	denotes	of
T8628	5911-5914	DT	denotes	the
T8629	5931-5943	NN	denotes	organization
T8630	5915-5930	NN	denotes	heterochromatin
T8631	5944-5949	VBN	denotes	found
T8632	5950-5952	IN	denotes	in
T8633	5953-5956	CD	denotes	two
T8634	5961-5965	CD	denotes	four
T8635	5956-5957	HYPH	denotes	-
T8636	5958-5960	IN	denotes	to
T8637	5966-5970	NN	denotes	cell
T8638	5965-5966	HYPH	denotes	-
T8639	5971-5976	NN	denotes	stage
T8640	5987-5994	NNS	denotes	embryos
T8641	5977-5981	JJ	denotes	wild
T8642	5982-5986	NN	denotes	type
T8643	5981-5982	HYPH	denotes	-
T8644	5995-5996	-LRB-	denotes	(
T8645	5996-6003	VB	denotes	compare
T8646	6004-6010	NN	denotes	Figure
T8647	6011-6013	NN	denotes	1D
T8648	6014-6017	CC	denotes	and
T8649	6018-6020	NN	denotes	1F
T8650	6020-6021	-RRB-	denotes	)
T8651	6021-6022	.	denotes	.
T8652	6022-6226	sentence	denotes	These data evidence a key role for p150CAF-1 during pre-implantation development, and reveal that this protein is required for the proper 3-D organization of heterochromatin within embryonic cell nuclei.
T8653	6023-6028	DT	denotes	These
T8654	6029-6033	NNS	denotes	data
T8655	6034-6042	VBP	denotes	evidence
T8656	6043-6044	DT	denotes	a
T8657	6049-6053	NN	denotes	role
T8658	6045-6048	JJ	denotes	key
T8659	6054-6057	IN	denotes	for
T8660	6058-6065	NN	denotes	p150CAF
T8661	6065-6066	HYPH	denotes	-
T8662	6066-6067	CD	denotes	1
T8663	6068-6074	IN	denotes	during
T8664	6075-6091	JJ	denotes	pre-implantation
T8665	6092-6103	NN	denotes	development
T8666	6103-6105	,	denotes	,
T8667	6105-6108	CC	denotes	and
T8668	6109-6115	VBP	denotes	reveal
T8669	6116-6120	IN	denotes	that
T8670	6137-6145	VBN	denotes	required
T8671	6121-6125	DT	denotes	this
T8672	6126-6133	NN	denotes	protein
T8673	6134-6136	VBZ	denotes	is
T8674	6146-6149	IN	denotes	for
T8675	6150-6153	DT	denotes	the
T8676	6165-6177	NN	denotes	organization
T8677	6154-6160	JJ	denotes	proper
T8678	6161-6162	CD	denotes	3
T8679	6163-6164	NN	denotes	D
T8680	6162-6163	HYPH	denotes	-
T8681	6178-6180	IN	denotes	of
T8682	6181-6196	NN	denotes	heterochromatin
T8683	6197-6203	IN	denotes	within
T8684	6204-6213	JJ	denotes	embryonic
T8685	6219-6225	NNS	denotes	nuclei
T8686	6214-6218	NN	denotes	cell
T8687	6225-6226	.	denotes	.
T8688	6226-6375	sentence	denotes	Next, we wondered whether a similar requirement for CAF-1 could also be observed in ES cells, which are derived from the blastocyst inner cell mass.
T8689	6227-6231	RB	denotes	Next
T8690	6236-6244	VBD	denotes	wondered
T8691	6231-6233	,	denotes	,
T8692	6233-6235	PRP	denotes	we
T8693	6245-6252	IN	denotes	whether
T8694	6299-6307	VBN	denotes	observed
T8695	6253-6254	DT	denotes	a
T8696	6263-6274	NN	denotes	requirement
T8697	6255-6262	JJ	denotes	similar
T8698	6275-6278	IN	denotes	for
T8699	6279-6282	NN	denotes	CAF
T8700	6282-6283	HYPH	denotes	-
T8701	6283-6284	CD	denotes	1
T8702	6285-6290	MD	denotes	could
T8703	6291-6295	RB	denotes	also
T8704	6296-6298	VB	denotes	be
T8705	6308-6310	IN	denotes	in
T8706	6311-6313	NN	denotes	ES
T8707	6314-6319	NNS	denotes	cells
T8708	6319-6321	,	denotes	,
T8709	6321-6326	WDT	denotes	which
T8710	6331-6338	VBN	denotes	derived
T8711	6327-6330	VBP	denotes	are
T8712	6339-6343	IN	denotes	from
T8713	6344-6347	DT	denotes	the
T8714	6370-6374	NN	denotes	mass
T8715	6348-6358	NN	denotes	blastocyst
T8716	6359-6364	JJ	denotes	inner
T8717	6365-6369	NN	denotes	cell
T8718	6374-6375	.	denotes	.
T8719	6375-6551	sentence	denotes	Given the early developmental arrest observed in Chaf1a −/− embryos, such cells could not be derived directly from null embryos, and we thus used an RNAi strategy (Figure 2A).
T8720	6376-6381	VBN	denotes	Given
T8721	6469-6476	VBN	denotes	derived
T8722	6382-6385	DT	denotes	the
T8723	6406-6412	NN	denotes	arrest
T8724	6386-6391	JJ	denotes	early
T8725	6392-6405	JJ	denotes	developmental
T8726	6413-6421	VBN	denotes	observed
T8727	6422-6424	IN	denotes	in
T8728	6425-6431	NN	denotes	Chaf1a
T8729	6436-6443	NNS	denotes	embryos
T8730	6432-6433	SYM	denotes	−
T8731	6433-6434	HYPH	denotes	/
T8732	6434-6435	SYM	denotes	−
T8733	6443-6445	,	denotes	,
T8734	6445-6449	JJ	denotes	such
T8735	6450-6455	NNS	denotes	cells
T8736	6456-6461	MD	denotes	could
T8737	6462-6465	RB	denotes	not
T8738	6466-6468	VB	denotes	be
T8739	6477-6485	RB	denotes	directly
T8740	6486-6490	IN	denotes	from
T8741	6491-6495	JJ	denotes	null
T8742	6496-6503	NNS	denotes	embryos
T8743	6503-6505	,	denotes	,
T8744	6505-6508	CC	denotes	and
T8745	6509-6511	PRP	denotes	we
T8746	6517-6521	VBD	denotes	used
T8747	6512-6516	RB	denotes	thus
T8748	6522-6524	DT	denotes	an
T8749	6530-6538	NN	denotes	strategy
T8750	6525-6529	NN	denotes	RNAi
T8751	6539-6540	-LRB-	denotes	(
T8752	6547-6549	NN	denotes	2A
T8753	6540-6546	NN	denotes	Figure
T8754	6549-6550	-RRB-	denotes	)
T8755	6550-6551	.	denotes	.
T8756	6551-6631	sentence	denotes	p150CAF-1 knockdown in ES cells was quantified by Western blot analysis and IF.
T8757	6552-6559	NN	denotes	p150CAF
T8758	6562-6571	NN	denotes	knockdown
T8759	6559-6560	HYPH	denotes	-
T8760	6560-6561	CD	denotes	1
T8761	6588-6598	VBN	denotes	quantified
T8762	6572-6574	IN	denotes	in
T8763	6575-6577	NN	denotes	ES
T8764	6578-6583	NNS	denotes	cells
T8765	6584-6587	VBD	denotes	was
T8766	6599-6601	IN	denotes	by
T8767	6602-6609	NNP	denotes	Western
T8768	6610-6614	NN	denotes	blot
T8769	6615-6623	NN	denotes	analysis
T8770	6624-6627	CC	denotes	and
T8771	6628-6630	NN	denotes	IF
T8772	6630-6631	.	denotes	.
T8773	6631-6644	sentence	denotes	(Figure S1).
T8774	6632-6633	-LRB-	denotes	(
T8775	6640-6642	NN	denotes	S1
T8776	6633-6639	NN	denotes	Figure
T8777	6642-6643	-RRB-	denotes	)
T8778	6643-6644	.	denotes	.
T8779	6644-6748	sentence	denotes	p150CAF-1 RNAi-depleted ES cells displayed a phenotype very similar to the cells of Chaf1a −/− E4 mice.
T8780	6645-6652	NN	denotes	p150CAF
T8781	6672-6677	NNS	denotes	cells
T8782	6652-6653	HYPH	denotes	-
T8783	6653-6654	CD	denotes	1
T8784	6655-6659	NN	denotes	RNAi
T8785	6660-6668	VBN	denotes	depleted
T8786	6659-6660	HYPH	denotes	-
T8787	6669-6671	NN	denotes	ES
T8788	6678-6687	VBD	denotes	displayed
T8789	6688-6689	DT	denotes	a
T8790	6690-6699	NN	denotes	phenotype
T8791	6700-6704	RB	denotes	very
T8792	6705-6712	JJ	denotes	similar
T8793	6713-6715	IN	denotes	to
T8794	6716-6719	DT	denotes	the
T8795	6720-6725	NNS	denotes	cells
T8796	6726-6728	IN	denotes	of
T8797	6729-6735	NN	denotes	Chaf1a
T8798	6743-6747	NNS	denotes	mice
T8799	6736-6737	SYM	denotes	−
T8800	6737-6738	HYPH	denotes	/
T8801	6738-6739	SYM	denotes	−
T8802	6740-6742	NN	denotes	E4
T8803	6747-6748	.	denotes	.
T8804	6748-6891	sentence	denotes	DAPI-dense foci were lost, and we observed diffuse HP1α and DAPI staining around the nucleoli and at the periphery of the nucleus (Figure 2B).
T8805	6749-6753	NN	denotes	DAPI
T8806	6754-6759	JJ	denotes	dense
T8807	6753-6754	HYPH	denotes	-
T8808	6760-6764	NNS	denotes	foci
T8809	6770-6774	VBN	denotes	lost
T8810	6765-6769	VBD	denotes	were
T8811	6774-6776	,	denotes	,
T8812	6776-6779	CC	denotes	and
T8813	6780-6782	PRP	denotes	we
T8814	6783-6791	VBD	denotes	observed
T8815	6792-6799	JJ	denotes	diffuse
T8816	6814-6822	NN	denotes	staining
T8817	6800-6804	NN	denotes	HP1α
T8818	6805-6808	CC	denotes	and
T8819	6809-6813	NN	denotes	DAPI
T8820	6823-6829	IN	denotes	around
T8821	6830-6833	DT	denotes	the
T8822	6834-6842	NNS	denotes	nucleoli
T8823	6843-6846	CC	denotes	and
T8824	6847-6849	IN	denotes	at
T8825	6850-6853	DT	denotes	the
T8826	6854-6863	NN	denotes	periphery
T8827	6864-6866	IN	denotes	of
T8828	6867-6870	DT	denotes	the
T8829	6871-6878	NN	denotes	nucleus
T8830	6879-6880	-LRB-	denotes	(
T8831	6887-6889	NN	denotes	2B
T8832	6880-6886	NN	denotes	Figure
T8833	6889-6890	-RRB-	denotes	)
T8834	6890-6891	.	denotes	.
T8835	6891-7049	sentence	denotes	This result indicates that p150CAF-1 is essential for nuclear organization of heterochromatin in ES cells in a similar way to early pre-implantation embryos.
T8836	6892-6896	DT	denotes	This
T8837	6897-6903	NN	denotes	result
T8838	6904-6913	VBZ	denotes	indicates
T8839	6914-6918	IN	denotes	that
T8840	6929-6931	VBZ	denotes	is
T8841	6919-6926	NN	denotes	p150CAF
T8842	6926-6927	HYPH	denotes	-
T8843	6927-6928	CD	denotes	1
T8844	6932-6941	JJ	denotes	essential
T8845	6942-6945	IN	denotes	for
T8846	6946-6953	JJ	denotes	nuclear
T8847	6954-6966	NN	denotes	organization
T8848	6967-6969	IN	denotes	of
T8849	6970-6985	NN	denotes	heterochromatin
T8850	6986-6988	IN	denotes	in
T8851	6989-6991	NN	denotes	ES
T8852	6992-6997	NNS	denotes	cells
T8853	6998-7000	IN	denotes	in
T8854	7001-7002	DT	denotes	a
T8855	7011-7014	NN	denotes	way
T8856	7003-7010	JJ	denotes	similar
T8857	7015-7017	IN	denotes	to
T8858	7018-7023	JJ	denotes	early
T8859	7041-7048	NNS	denotes	embryos
T8860	7024-7040	JJ	denotes	pre-implantation
T8861	7048-7049	.	denotes	.
T8862	7049-7222	sentence	denotes	Surprisingly, we did not observe this severe alteration in heterochromatin organization in primary mouse embryonic fibroblasts (MEFs) following p150CAF-1 depletion by RNAi.
T8863	7050-7062	RB	denotes	Surprisingly
T8864	7075-7082	VB	denotes	observe
T8865	7062-7064	,	denotes	,
T8866	7064-7066	PRP	denotes	we
T8867	7067-7070	VBD	denotes	did
T8868	7071-7074	RB	denotes	not
T8869	7083-7087	DT	denotes	this
T8870	7095-7105	NN	denotes	alteration
T8871	7088-7094	JJ	denotes	severe
T8872	7106-7108	IN	denotes	in
T8873	7109-7124	NN	denotes	heterochromatin
T8874	7125-7137	NN	denotes	organization
T8875	7138-7140	IN	denotes	in
T8876	7141-7148	JJ	denotes	primary
T8877	7165-7176	NNS	denotes	fibroblasts
T8878	7149-7154	NN	denotes	mouse
T8879	7155-7164	JJ	denotes	embryonic
T8880	7177-7178	-LRB-	denotes	(
T8881	7178-7182	NNS	denotes	MEFs
T8882	7182-7183	-RRB-	denotes	)
T8883	7184-7193	VBG	denotes	following
T8884	7194-7201	NN	denotes	p150CAF
T8885	7204-7213	NN	denotes	depletion
T8886	7201-7202	HYPH	denotes	-
T8887	7202-7203	CD	denotes	1
T8888	7214-7216	IN	denotes	by
T8889	7217-7221	NN	denotes	RNAi
T8890	7221-7222	.	denotes	.
T8891	7222-7405	sentence	denotes	p150CAF-1 depletion in MEFs resulted in a strong inhibition of cell proliferation (unpublished data), but did not alter the clustering of heterochromatin domains (Figures 2C and S2).
T8892	7223-7230	NN	denotes	p150CAF
T8893	7233-7242	NN	denotes	depletion
T8894	7230-7231	HYPH	denotes	-
T8895	7231-7232	CD	denotes	1
T8896	7251-7259	VBD	denotes	resulted
T8897	7243-7245	IN	denotes	in
T8898	7246-7250	NNS	denotes	MEFs
T8899	7260-7262	IN	denotes	in
T8900	7263-7264	DT	denotes	a
T8901	7272-7282	NN	denotes	inhibition
T8902	7265-7271	JJ	denotes	strong
T8903	7283-7285	IN	denotes	of
T8904	7286-7290	NN	denotes	cell
T8905	7291-7304	NN	denotes	proliferation
T8906	7305-7306	-LRB-	denotes	(
T8907	7318-7322	NNS	denotes	data
T8908	7306-7317	JJ	denotes	unpublished
T8909	7322-7323	-RRB-	denotes	)
T8910	7323-7325	,	denotes	,
T8911	7325-7328	CC	denotes	but
T8912	7329-7332	VBD	denotes	did
T8913	7337-7342	VB	denotes	alter
T8914	7333-7336	RB	denotes	not
T8915	7343-7346	DT	denotes	the
T8916	7347-7357	NN	denotes	clustering
T8917	7358-7360	IN	denotes	of
T8918	7361-7376	NN	denotes	heterochromatin
T8919	7377-7384	NNS	denotes	domains
T8920	7385-7386	-LRB-	denotes	(
T8921	7394-7396	NN	denotes	2C
T8922	7386-7393	NNS	denotes	Figures
T8923	7397-7400	CC	denotes	and
T8924	7401-7403	NN	denotes	S2
T8925	7403-7404	-RRB-	denotes	)
T8926	7404-7405	.	denotes	.
T8927	7405-7499	sentence	denotes	Similarly, p150CAF-1 depletion in 3T3 cells did not affect heterochromatin organization [16].
T8928	7406-7415	RB	denotes	Similarly
T8929	7458-7464	VB	denotes	affect
T8930	7415-7417	,	denotes	,
T8931	7417-7424	NN	denotes	p150CAF
T8932	7427-7436	NN	denotes	depletion
T8933	7424-7425	HYPH	denotes	-
T8934	7425-7426	CD	denotes	1
T8935	7437-7439	IN	denotes	in
T8936	7440-7443	NN	denotes	3T3
T8937	7444-7449	NNS	denotes	cells
T8938	7450-7453	VBD	denotes	did
T8939	7454-7457	RB	denotes	not
T8940	7465-7480	NN	denotes	heterochromatin
T8941	7481-7493	NN	denotes	organization
T8942	7494-7495	-LRB-	denotes	[
T8943	7495-7497	CD	denotes	16
T8944	7497-7498	-RRB-	denotes	]
T8945	7498-7499	.	denotes	.
T8946	7499-7589	sentence	denotes	These observations reveal a specific function for CAF-1 in the early embryo and ES cells.
T8947	7500-7505	DT	denotes	These
T8948	7506-7518	NNS	denotes	observations
T8949	7519-7525	VBP	denotes	reveal
T8950	7526-7527	DT	denotes	a
T8951	7537-7545	NN	denotes	function
T8952	7528-7536	JJ	denotes	specific
T8953	7546-7549	IN	denotes	for
T8954	7550-7553	NN	denotes	CAF
T8955	7553-7554	HYPH	denotes	-
T8956	7554-7555	CD	denotes	1
T8957	7556-7558	IN	denotes	in
T8958	7559-7562	DT	denotes	the
T8959	7569-7575	NN	denotes	embryo
T8960	7563-7568	JJ	denotes	early
T8961	7576-7579	CC	denotes	and
T8962	7580-7582	NN	denotes	ES
T8963	7583-7588	NNS	denotes	cells
T8964	7588-7589	.	denotes	.
T8965	7589-9072	sentence	denotes	Figure 2 Depletion of p150CAF-1 in ES Cells Results in a Severe Alteration of Heterochromatin Organization (A) Strategy used to deplete p150CAF-1 by RNAi in ES cells. The siRNA expression vector includes a puromycin selection cassette (Puro), the mouse H1 promoter, and the siRNA encoding sequence. ES cells were kept under puromycin selection during 48 h following transfection. (B) Abnormal heterochromatin organization in p150CAF-1-depleted ES cells. Immunodetection of p150CAF-1 (green) and HP1α (red) in ES cells transfected with control (cont) and p150CAF-1 siRNA. siRNA expression results in efficient p150CAF-1 depletion. The right-hand image shows the merge between HP1α fluorescence and DAPI-stained DNA in blue. Scale bar = 10 μm. (C) Heterochromatin organization is not altered in p150CAF-1-depleted MEFs. Immunodetection of p150CAF-1 (green) and HP1α (red) in MEFs transfected with control (cont) or p150CAF-1 siRNA. The right-hand image shows the merge between HP1α fluorescence and DAPI-stained DNA in blue. (D) PML bodies are not altered in p150CAF-1-depleted ES cells. Immunodetection of PML (red) in ES cells transfected with control (cont) or p150CAF-1 siRNA. The right-hand image shows the merge between PML fluorescence and DAPI-stained DNA in blue. We also wondered whether loss of p150CAF-1 in ES cells specifically affects the heterochromatin nuclear subcompartment, or whether it might result in a more global loss of nuclear organization and architecture.
T8966	8862-8864	PRP	denotes	We
T8967	8870-8878	VBD	denotes	wondered
T8968	8865-8869	RB	denotes	also
T8969	8879-8886	IN	denotes	whether
T8970	8930-8937	VBZ	denotes	affects
T8971	8887-8891	NN	denotes	loss
T8972	8892-8894	IN	denotes	of
T8973	8895-8902	NN	denotes	p150CAF
T8974	8902-8903	HYPH	denotes	-
T8975	8903-8904	CD	denotes	1
T8976	8905-8907	IN	denotes	in
T8977	8908-8910	NN	denotes	ES
T8978	8911-8916	NNS	denotes	cells
T8979	8917-8929	RB	denotes	specifically
T8980	8938-8941	DT	denotes	the
T8981	8966-8980	NN	denotes	subcompartment
T8982	8942-8957	NN	denotes	heterochromatin
T8983	8958-8965	JJ	denotes	nuclear
T8984	8980-8982	,	denotes	,
T8985	8982-8984	CC	denotes	or
T8986	8985-8992	IN	denotes	whether
T8987	9002-9008	VB	denotes	result
T8988	8993-8995	PRP	denotes	it
T8989	8996-9001	MD	denotes	might
T8990	9009-9011	IN	denotes	in
T8991	9012-9013	DT	denotes	a
T8992	9026-9030	NN	denotes	loss
T8993	9014-9018	RBR	denotes	more
T8994	9019-9025	JJ	denotes	global
T8995	9031-9033	IN	denotes	of
T8996	9034-9041	JJ	denotes	nuclear
T8997	9042-9054	NN	denotes	organization
T8998	9055-9058	CC	denotes	and
T8999	9059-9071	NN	denotes	architecture
T9000	9071-9072	.	denotes	.
T9001	9072-9251	sentence	denotes	We used a specific antibody against the promyelocytic leukemia (PML) protein to study the fate of the PML nuclear bodies, which are well-characterized subnuclear structures [22].
T9002	9073-9075	PRP	denotes	We
T9003	9076-9080	VBD	denotes	used
T9004	9081-9082	DT	denotes	a
T9005	9092-9100	NN	denotes	antibody
T9006	9083-9091	JJ	denotes	specific
T9007	9101-9108	IN	denotes	against
T9008	9109-9112	DT	denotes	the
T9009	9142-9149	NN	denotes	protein
T9010	9113-9126	JJ	denotes	promyelocytic
T9011	9127-9135	NN	denotes	leukemia
T9012	9136-9137	-LRB-	denotes	(
T9013	9137-9140	NN	denotes	PML
T9014	9140-9141	-RRB-	denotes	)
T9015	9150-9152	TO	denotes	to
T9016	9153-9158	VB	denotes	study
T9017	9159-9162	DT	denotes	the
T9018	9163-9167	NN	denotes	fate
T9019	9168-9170	IN	denotes	of
T9020	9171-9174	DT	denotes	the
T9021	9187-9193	NNS	denotes	bodies
T9022	9175-9178	NN	denotes	PML
T9023	9179-9186	JJ	denotes	nuclear
T9024	9193-9195	,	denotes	,
T9025	9195-9200	WDT	denotes	which
T9026	9201-9204	VBP	denotes	are
T9027	9205-9209	RB	denotes	well
T9028	9210-9223	VBN	denotes	characterized
T9029	9209-9210	HYPH	denotes	-
T9030	9235-9245	NNS	denotes	structures
T9031	9224-9234	JJ	denotes	subnuclear
T9032	9246-9247	-LRB-	denotes	[
T9033	9247-9249	CD	denotes	22
T9034	9249-9250	-RRB-	denotes	]
T9035	9250-9251	.	denotes	.
T9036	9251-9412	sentence	denotes	We could not detect any significant difference in the distribution and aspect of PML nuclear bodies between control and p150CAF-1-depleted ES cells (Figure 2D).
T9037	9252-9254	PRP	denotes	We
T9038	9265-9271	VB	denotes	detect
T9039	9255-9260	MD	denotes	could
T9040	9261-9264	RB	denotes	not
T9041	9272-9275	DT	denotes	any
T9042	9288-9298	NN	denotes	difference
T9043	9276-9287	JJ	denotes	significant
T9044	9299-9301	IN	denotes	in
T9045	9302-9305	DT	denotes	the
T9046	9306-9318	NN	denotes	distribution
T9047	9319-9322	CC	denotes	and
T9048	9323-9329	NN	denotes	aspect
T9049	9330-9332	IN	denotes	of
T9050	9333-9336	NN	denotes	PML
T9051	9345-9351	NNS	denotes	bodies
T9052	9337-9344	JJ	denotes	nuclear
T9053	9352-9359	IN	denotes	between
T9054	9360-9367	NN	denotes	control
T9055	9394-9399	NNS	denotes	cells
T9056	9368-9371	CC	denotes	and
T9057	9372-9379	NN	denotes	p150CAF
T9058	9382-9390	VBN	denotes	depleted
T9059	9379-9380	HYPH	denotes	-
T9060	9380-9381	CD	denotes	1
T9061	9381-9382	HYPH	denotes	-
T9062	9391-9393	NN	denotes	ES
T9063	9400-9401	-LRB-	denotes	(
T9064	9408-9410	NN	denotes	2D
T9065	9401-9407	NN	denotes	Figure
T9066	9410-9411	-RRB-	denotes	)
T9067	9411-9412	.	denotes	.
T9068	9412-9512	sentence	denotes	These data show that nuclear architecture is not globally altered following CAF-1 loss-of-function.
T9069	9413-9418	DT	denotes	These
T9070	9419-9423	NNS	denotes	data
T9071	9424-9428	VBP	denotes	show
T9072	9429-9433	IN	denotes	that
T9073	9471-9478	VBN	denotes	altered
T9074	9434-9441	JJ	denotes	nuclear
T9075	9442-9454	NN	denotes	architecture
T9076	9455-9457	VBZ	denotes	is
T9077	9458-9461	RB	denotes	not
T9078	9462-9470	RB	denotes	globally
T9079	9479-9488	VBG	denotes	following
T9080	9489-9492	NN	denotes	CAF
T9081	9492-9493	HYPH	denotes	-
T9082	9493-9494	CD	denotes	1
T9083	9495-9499	NN	denotes	loss
T9084	9499-9500	HYPH	denotes	-
T9085	9500-9502	IN	denotes	of
T9086	9502-9503	HYPH	denotes	-
T9087	9503-9511	NN	denotes	function
T9088	9511-9512	.	denotes	.
T9089	9512-9687	sentence	denotes	In addition, Western blot analysis revealed that CAF-1 depletion did not result in altered levels of chromatin architectural proteins such as histone H3 and HP1α (Figure S1).
T9090	9513-9515	IN	denotes	In
T9091	9548-9556	VBD	denotes	revealed
T9092	9516-9524	NN	denotes	addition
T9093	9524-9526	,	denotes	,
T9094	9526-9533	NNP	denotes	Western
T9095	9534-9538	NN	denotes	blot
T9096	9539-9547	NN	denotes	analysis
T9097	9557-9561	IN	denotes	that
T9098	9586-9592	VB	denotes	result
T9099	9562-9565	NN	denotes	CAF
T9100	9568-9577	NN	denotes	depletion
T9101	9565-9566	HYPH	denotes	-
T9102	9566-9567	CD	denotes	1
T9103	9578-9581	VBD	denotes	did
T9104	9582-9585	RB	denotes	not
T9105	9593-9595	IN	denotes	in
T9106	9596-9603	VBN	denotes	altered
T9107	9604-9610	NNS	denotes	levels
T9108	9611-9613	IN	denotes	of
T9109	9614-9623	NN	denotes	chromatin
T9110	9638-9646	NN	denotes	proteins
T9111	9624-9637	JJ	denotes	architectural
T9112	9647-9651	JJ	denotes	such
T9113	9652-9654	IN	denotes	as
T9114	9655-9662	NN	denotes	histone
T9115	9663-9665	NN	denotes	H3
T9116	9666-9669	CC	denotes	and
T9117	9670-9674	NN	denotes	HP1α
T9118	9675-9676	-LRB-	denotes	(
T9119	9683-9685	NN	denotes	S1
T9120	9676-9682	NN	denotes	Figure
T9121	9685-9686	-RRB-	denotes	)
T9122	9686-9687	.	denotes	.
T9123	9687-9832	sentence	denotes	Thus, CAF-1 is specifically required in early embryos and in ES cells for the proper organization of the heterochromatin subnuclear compartment.
T9124	9688-9692	RB	denotes	Thus
T9125	9716-9724	VBN	denotes	required
T9126	9692-9694	,	denotes	,
T9127	9694-9697	NN	denotes	CAF
T9128	9697-9698	HYPH	denotes	-
T9129	9698-9699	CD	denotes	1
T9130	9700-9702	VBZ	denotes	is
T9131	9703-9715	RB	denotes	specifically
T9132	9725-9727	IN	denotes	in
T9133	9728-9733	JJ	denotes	early
T9134	9734-9741	NNS	denotes	embryos
T9135	9742-9745	CC	denotes	and
T9136	9746-9748	IN	denotes	in
T9137	9749-9751	NN	denotes	ES
T9138	9752-9757	NNS	denotes	cells
T9139	9758-9761	IN	denotes	for
T9140	9762-9765	DT	denotes	the
T9141	9773-9785	NN	denotes	organization
T9142	9766-9772	JJ	denotes	proper
T9143	9786-9788	IN	denotes	of
T9144	9789-9792	DT	denotes	the
T9145	9820-9831	NN	denotes	compartment
T9146	9793-9808	NN	denotes	heterochromatin
T9147	9809-9819	JJ	denotes	subnuclear
T9148	9831-9832	.	denotes	.
T9149	9832-9958	sentence	denotes	Previous studies performed in mammalian cell lines showed that CAF-1 activity is required for S phase progression [16,23,24].
T9150	9833-9841	JJ	denotes	Previous
T9151	9842-9849	NNS	denotes	studies
T9152	9884-9890	VBD	denotes	showed
T9153	9850-9859	VBN	denotes	performed
T9154	9860-9862	IN	denotes	in
T9155	9863-9872	JJ	denotes	mammalian
T9156	9878-9883	NNS	denotes	lines
T9157	9873-9877	NN	denotes	cell
T9158	9891-9895	IN	denotes	that
T9159	9914-9922	VBN	denotes	required
T9160	9896-9899	NN	denotes	CAF
T9161	9902-9910	NN	denotes	activity
T9162	9899-9900	HYPH	denotes	-
T9163	9900-9901	CD	denotes	1
T9164	9911-9913	VBZ	denotes	is
T9165	9923-9926	IN	denotes	for
T9166	9927-9928	NN	denotes	S
T9167	9929-9934	NN	denotes	phase
T9168	9935-9946	NN	denotes	progression
T9169	9947-9948	-LRB-	denotes	[
T9170	9954-9956	CD	denotes	24
T9171	9948-9950	CD	denotes	16
T9172	9950-9951	,	denotes	,
T9173	9951-9953	CD	denotes	23
T9174	9953-9954	,	denotes	,
T9175	9956-9957	-RRB-	denotes	]
T9176	9957-9958	.	denotes	.
T9177	9958-10334	sentence	denotes	We show here that 3 d after transfection of the RNAi vector, p150CAF-1-depleted cells (identified by a complete absence of CAF-1 IF signal) appear still active for replication, as revealed by incorporation of the thymidine analog bromodeoxyuridine (BrdU) (Figure 3A), the PCNA (proliferating cell nuclear antigen) pattern (Figure 3B), and flow cytometry analysis (Figure 3C).
T9178	9959-9961	PRP	denotes	We
T9179	9962-9966	VBP	denotes	show
T9180	9967-9971	RB	denotes	here
T9181	9972-9976	IN	denotes	that
T9182	10099-10105	VBP	denotes	appear
T9183	9977-9978	CD	denotes	3
T9184	9979-9980	NN	denotes	d
T9185	9981-9986	IN	denotes	after
T9186	9987-9999	NN	denotes	transfection
T9187	10000-10002	IN	denotes	of
T9188	10003-10006	DT	denotes	the
T9189	10012-10018	NN	denotes	vector
T9190	10007-10011	NN	denotes	RNAi
T9191	10018-10020	,	denotes	,
T9192	10020-10027	NN	denotes	p150CAF
T9193	10030-10038	VBN	denotes	depleted
T9194	10027-10028	HYPH	denotes	-
T9195	10028-10029	CD	denotes	1
T9196	10029-10030	HYPH	denotes	-
T9197	10039-10044	NNS	denotes	cells
T9198	10045-10046	-LRB-	denotes	(
T9199	10046-10056	VBN	denotes	identified
T9200	10057-10059	IN	denotes	by
T9201	10060-10061	DT	denotes	a
T9202	10071-10078	NN	denotes	absence
T9203	10062-10070	JJ	denotes	complete
T9204	10079-10081	IN	denotes	of
T9205	10082-10085	NN	denotes	CAF
T9206	10091-10097	NN	denotes	signal
T9207	10085-10086	HYPH	denotes	-
T9208	10086-10087	CD	denotes	1
T9209	10088-10090	NN	denotes	IF
T9210	10097-10098	-RRB-	denotes	)
T9211	10106-10111	RB	denotes	still
T9212	10112-10118	JJ	denotes	active
T9213	10119-10122	IN	denotes	for
T9214	10123-10134	NN	denotes	replication
T9215	10134-10136	,	denotes	,
T9216	10136-10138	IN	denotes	as
T9217	10139-10147	VBN	denotes	revealed
T9218	10148-10150	IN	denotes	by
T9219	10151-10164	NN	denotes	incorporation
T9220	10165-10167	IN	denotes	of
T9221	10168-10171	DT	denotes	the
T9222	10182-10188	NN	denotes	analog
T9223	10172-10181	NN	denotes	thymidine
T9224	10189-10206	NN	denotes	bromodeoxyuridine
T9225	10207-10208	-LRB-	denotes	(
T9226	10208-10212	NN	denotes	BrdU
T9227	10212-10213	-RRB-	denotes	)
T9228	10214-10215	-LRB-	denotes	(
T9229	10222-10224	NN	denotes	3A
T9230	10215-10221	NN	denotes	Figure
T9231	10224-10225	-RRB-	denotes	)
T9232	10225-10227	,	denotes	,
T9233	10227-10230	DT	denotes	the
T9234	10273-10280	NN	denotes	pattern
T9235	10231-10235	NN	denotes	PCNA
T9236	10236-10237	-LRB-	denotes	(
T9237	10237-10250	VBG	denotes	proliferating
T9238	10264-10271	NN	denotes	antigen
T9239	10251-10255	NN	denotes	cell
T9240	10256-10263	JJ	denotes	nuclear
T9241	10271-10272	-RRB-	denotes	)
T9242	10281-10282	-LRB-	denotes	(
T9243	10289-10291	NN	denotes	3B
T9244	10282-10288	NN	denotes	Figure
T9245	10291-10292	-RRB-	denotes	)
T9246	10292-10294	,	denotes	,
T9247	10294-10297	CC	denotes	and
T9248	10298-10302	NN	denotes	flow
T9249	10313-10321	NN	denotes	analysis
T9250	10303-10312	NN	denotes	cytometry
T9251	10322-10323	-LRB-	denotes	(
T9252	10330-10332	NN	denotes	3C
T9253	10323-10329	NN	denotes	Figure
T9254	10332-10333	-RRB-	denotes	)
T9255	10333-10334	.	denotes	.
T9256	10334-10443	sentence	denotes	Hence, in these cells, DNA replication persists despite the drastic changes in heterochromatin organization.
T9257	10335-10340	RB	denotes	Hence
T9258	10374-10382	VBZ	denotes	persists
T9259	10340-10342	,	denotes	,
T9260	10342-10344	IN	denotes	in
T9261	10345-10350	DT	denotes	these
T9262	10351-10356	NNS	denotes	cells
T9263	10356-10358	,	denotes	,
T9264	10358-10361	NN	denotes	DNA
T9265	10362-10373	NN	denotes	replication
T9266	10383-10390	IN	denotes	despite
T9267	10391-10394	DT	denotes	the
T9268	10403-10410	NNS	denotes	changes
T9269	10395-10402	JJ	denotes	drastic
T9270	10411-10413	IN	denotes	in
T9271	10414-10429	NN	denotes	heterochromatin
T9272	10430-10442	NN	denotes	organization
T9273	10442-10443	.	denotes	.
T9274	10443-10590	sentence	denotes	However, 24 h later, ES cells cease to proliferate and die, revealing that p150CAF-1 is required for an essential cellular process, as in embryos.
T9275	10444-10451	RB	denotes	However
T9276	10474-10479	VBP	denotes	cease
T9277	10451-10453	,	denotes	,
T9278	10453-10455	CD	denotes	24
T9279	10456-10457	NN	denotes	h
T9280	10458-10463	RB	denotes	later
T9281	10463-10465	,	denotes	,
T9282	10465-10467	NN	denotes	ES
T9283	10468-10473	NNS	denotes	cells
T9284	10480-10482	TO	denotes	to
T9285	10483-10494	VB	denotes	proliferate
T9286	10495-10498	CC	denotes	and
T9287	10499-10502	VB	denotes	die
T9288	10502-10504	,	denotes	,
T9289	10504-10513	VBG	denotes	revealing
T9290	10514-10518	IN	denotes	that
T9291	10532-10540	VBN	denotes	required
T9292	10519-10526	NN	denotes	p150CAF
T9293	10526-10527	HYPH	denotes	-
T9294	10527-10528	CD	denotes	1
T9295	10529-10531	VBZ	denotes	is
T9296	10541-10544	IN	denotes	for
T9297	10545-10547	DT	denotes	an
T9298	10567-10574	NN	denotes	process
T9299	10548-10557	JJ	denotes	essential
T9300	10558-10566	JJ	denotes	cellular
T9301	10574-10576	,	denotes	,
T9302	10576-10578	IN	denotes	as
T9303	10579-10581	IN	denotes	in
T9304	10582-10589	NNS	denotes	embryos
T9305	10589-10590	.	denotes	.
T9306	10590-10802	sentence	denotes	Altogether, our data demonstrate that loss of p150CAF-1 function in ES cells and early embryos alters first the organization of heterochromatin in the nucleus and, in a subsequent step, cell cycle and viability.
T9307	10591-10601	RB	denotes	Altogether
T9308	10612-10623	VBP	denotes	demonstrate
T9309	10601-10603	,	denotes	,
T9310	10603-10606	PRP$	denotes	our
T9311	10607-10611	NNS	denotes	data
T9312	10624-10628	IN	denotes	that
T9313	10686-10692	VBZ	denotes	alters
T9314	10629-10633	NN	denotes	loss
T9315	10634-10636	IN	denotes	of
T9316	10637-10644	NN	denotes	p150CAF
T9317	10647-10655	NN	denotes	function
T9318	10644-10645	HYPH	denotes	-
T9319	10645-10646	CD	denotes	1
T9320	10656-10658	IN	denotes	in
T9321	10659-10661	NN	denotes	ES
T9322	10662-10667	NNS	denotes	cells
T9323	10668-10671	CC	denotes	and
T9324	10672-10677	JJ	denotes	early
T9325	10678-10685	NNS	denotes	embryos
T9326	10693-10698	RB	denotes	first
T9327	10699-10702	DT	denotes	the
T9328	10703-10715	NN	denotes	organization
T9329	10716-10718	IN	denotes	of
T9330	10719-10734	NN	denotes	heterochromatin
T9331	10735-10737	IN	denotes	in
T9332	10738-10741	DT	denotes	the
T9333	10742-10749	NN	denotes	nucleus
T9334	10750-10753	CC	denotes	and
T9335	10753-10755	,	denotes	,
T9336	10755-10757	IN	denotes	in
T9337	10782-10787	NN	denotes	cycle
T9338	10758-10759	DT	denotes	a
T9339	10771-10775	NN	denotes	step
T9340	10760-10770	JJ	denotes	subsequent
T9341	10775-10777	,	denotes	,
T9342	10777-10781	NN	denotes	cell
T9343	10788-10791	CC	denotes	and
T9344	10792-10801	NN	denotes	viability
T9345	10801-10802	.	denotes	.
T9346	10802-12372	sentence	denotes	Figure 3 p150CAF-1 Depletion and Loss of Heterochromatin Organization Are Compatible with Active DNA Replication in ES Cells (A) ES cells were transfected with p150CAF-1 or control siRNA vectors. After 3 d under puromycin selection, cells were pulse-labeled for 10 min with BrdU and immediately analyzed by IF. No significant difference in BrdU incorporation could be detected between control and p150CAF-1-depleted cells. The right-hand image shows the merge between the p150CAF-1 and BrdU fluorescence. Scale bar = 10 μm. (B) Immunodetection of PCNA (red) revealed no significant difference in the formation of replication foci between control and p150CAF-1-depleted ES cells. Immunodetection of p150CAF-1 is shown in green and the merging of p150CAF-1 and PCNA appears in yellow. (C) Flow cytometry analysis showed a similar cell-cycle profile for control and p150CAF-1-depleted ES cells. Results are presented as the percentage of cells in each phase of the cell cycle (G1, S, G2/M), as defined by BrdU incorporation and DNA content. Data presented are the mean of three independent experiments; error bars indicate the standard deviation. All experiments were performed 3 d after transfection of the siRNA vectors. In order to better characterize the defects in heterochromatin organization, we performed two-color DNA fluorescence in situ hybridization (FISH) experiments to reveal the spatial distribution of pericentric and centric chromosomal regions, which in mouse are mainly composed of large blocks of major and minor satellite repeats, respectively [20].
T9347	12024-12026	IN	denotes	In
T9348	12104-12113	VBD	denotes	performed
T9349	12027-12032	NN	denotes	order
T9350	12033-12035	TO	denotes	to
T9351	12043-12055	VB	denotes	characterize
T9352	12036-12042	RBR	denotes	better
T9353	12056-12059	DT	denotes	the
T9354	12060-12067	NNS	denotes	defects
T9355	12068-12070	IN	denotes	in
T9356	12071-12086	NN	denotes	heterochromatin
T9357	12087-12099	NN	denotes	organization
T9358	12099-12101	,	denotes	,
T9359	12101-12103	PRP	denotes	we
T9360	12114-12117	CD	denotes	two
T9361	12118-12123	NN	denotes	color
T9362	12117-12118	HYPH	denotes	-
T9363	12170-12181	NNS	denotes	experiments
T9364	12124-12127	NN	denotes	DNA
T9365	12128-12140	NN	denotes	fluorescence
T9366	12149-12162	NN	denotes	hybridization
T9367	12141-12143	FW	denotes	in
T9368	12144-12148	FW	denotes	situ
T9369	12163-12164	-LRB-	denotes	(
T9370	12164-12168	NN	denotes	FISH
T9371	12168-12169	-RRB-	denotes	)
T9372	12182-12184	TO	denotes	to
T9373	12185-12191	VB	denotes	reveal
T9374	12192-12195	DT	denotes	the
T9375	12204-12216	NN	denotes	distribution
T9376	12196-12203	JJ	denotes	spatial
T9377	12217-12219	IN	denotes	of
T9378	12220-12231	JJ	denotes	pericentric
T9379	12256-12263	NNS	denotes	regions
T9380	12232-12235	CC	denotes	and
T9381	12236-12243	JJ	denotes	centric
T9382	12244-12255	JJ	denotes	chromosomal
T9383	12263-12265	,	denotes	,
T9384	12265-12270	WDT	denotes	which
T9385	12291-12299	VBN	denotes	composed
T9386	12271-12273	IN	denotes	in
T9387	12274-12279	NN	denotes	mouse
T9388	12280-12283	VBP	denotes	are
T9389	12284-12290	RB	denotes	mainly
T9390	12300-12302	IN	denotes	of
T9391	12303-12308	JJ	denotes	large
T9392	12309-12315	NNS	denotes	blocks
T9393	12316-12318	IN	denotes	of
T9394	12319-12324	JJ	denotes	major
T9395	12345-12352	NNS	denotes	repeats
T9396	12325-12328	CC	denotes	and
T9397	12329-12334	JJ	denotes	minor
T9398	12335-12344	NN	denotes	satellite
T9399	12352-12354	,	denotes	,
T9400	12354-12366	RB	denotes	respectively
T9401	12367-12368	-LRB-	denotes	[
T9402	12368-12370	CD	denotes	20
T9403	12370-12371	-RRB-	denotes	]
T9404	12371-12372	.	denotes	.
T9405	12372-12550	sentence	denotes	Pericentric domains from different chromosomes form clusters, which are revealed by FISH as large spots that coincide with DAPI-dense foci in the interphase nucleus (Figure 4A).
T9406	12373-12384	JJ	denotes	Pericentric
T9407	12385-12392	NNS	denotes	domains
T9408	12420-12424	VBP	denotes	form
T9409	12393-12397	IN	denotes	from
T9410	12398-12407	JJ	denotes	different
T9411	12408-12419	NNS	denotes	chromosomes
T9412	12425-12433	NNS	denotes	clusters
T9413	12433-12435	,	denotes	,
T9414	12435-12440	WDT	denotes	which
T9415	12445-12453	VBN	denotes	revealed
T9416	12441-12444	VBP	denotes	are
T9417	12454-12456	IN	denotes	by
T9418	12457-12461	NN	denotes	FISH
T9419	12462-12464	IN	denotes	as
T9420	12465-12470	JJ	denotes	large
T9421	12471-12476	NNS	denotes	spots
T9422	12477-12481	WDT	denotes	that
T9423	12482-12490	VBP	denotes	coincide
T9424	12491-12495	IN	denotes	with
T9425	12496-12500	NN	denotes	DAPI
T9426	12501-12506	JJ	denotes	dense
T9427	12500-12501	HYPH	denotes	-
T9428	12507-12511	NNS	denotes	foci
T9429	12512-12514	IN	denotes	in
T9430	12515-12518	DT	denotes	the
T9431	12530-12537	NN	denotes	nucleus
T9432	12519-12529	NN	denotes	interphase
T9433	12538-12539	-LRB-	denotes	(
T9434	12546-12548	NN	denotes	4A
T9435	12539-12545	NN	denotes	Figure
T9436	12548-12549	-RRB-	denotes	)
T9437	12549-12550	.	denotes	.
T9438	12550-12649	sentence	denotes	At the periphery of each pericentric domain, centric regions form individual entities (Figure 4A).
T9439	12551-12553	IN	denotes	At
T9440	12612-12616	VBP	denotes	form
T9441	12554-12557	DT	denotes	the
T9442	12558-12567	NN	denotes	periphery
T9443	12568-12570	IN	denotes	of
T9444	12571-12575	DT	denotes	each
T9445	12588-12594	NN	denotes	domain
T9446	12576-12587	JJ	denotes	pericentric
T9447	12594-12596	,	denotes	,
T9448	12596-12603	JJ	denotes	centric
T9449	12604-12611	NNS	denotes	regions
T9450	12617-12627	JJ	denotes	individual
T9451	12628-12636	NNS	denotes	entities
T9452	12637-12638	-LRB-	denotes	(
T9453	12645-12647	NN	denotes	4A
T9454	12638-12644	NN	denotes	Figure
T9455	12647-12648	-RRB-	denotes	)
T9456	12648-12649	.	denotes	.
T9457	12649-12818	sentence	denotes	In p150CAF-1 RNAi-depleted cells, we observed a disruption of pericentric heterochromatin clusters that coincides with the disappearance of DAPI-dense foci (Figure 4B).
T9458	12650-12652	IN	denotes	In
T9459	12687-12695	VBD	denotes	observed
T9460	12653-12660	NN	denotes	p150CAF
T9461	12677-12682	NNS	denotes	cells
T9462	12660-12661	HYPH	denotes	-
T9463	12661-12662	CD	denotes	1
T9464	12663-12667	NN	denotes	RNAi
T9465	12668-12676	VBN	denotes	depleted
T9466	12667-12668	HYPH	denotes	-
T9467	12682-12684	,	denotes	,
T9468	12684-12686	PRP	denotes	we
T9469	12696-12697	DT	denotes	a
T9470	12698-12708	NN	denotes	disruption
T9471	12709-12711	IN	denotes	of
T9472	12712-12723	JJ	denotes	pericentric
T9473	12740-12748	NNS	denotes	clusters
T9474	12724-12739	NN	denotes	heterochromatin
T9475	12749-12753	WDT	denotes	that
T9476	12754-12763	VBZ	denotes	coincides
T9477	12764-12768	IN	denotes	with
T9478	12769-12772	DT	denotes	the
T9479	12773-12786	NN	denotes	disappearance
T9480	12787-12789	IN	denotes	of
T9481	12790-12794	NN	denotes	DAPI
T9482	12795-12800	JJ	denotes	dense
T9483	12794-12795	HYPH	denotes	-
T9484	12801-12805	NNS	denotes	foci
T9485	12806-12807	-LRB-	denotes	(
T9486	12814-12816	NN	denotes	4B
T9487	12807-12813	NN	denotes	Figure
T9488	12816-12817	-RRB-	denotes	)
T9489	12817-12818	.	denotes	.
T9490	12818-13006	sentence	denotes	Individual pericentric domains from single chromosomes are now found either isolated or aggregated in a pattern less dense than the regular clusters observed in control cells (Figure 4B).
T9491	12819-12829	JJ	denotes	Individual
T9492	12842-12849	NNS	denotes	domains
T9493	12830-12841	JJ	denotes	pericentric
T9494	12882-12887	VBN	denotes	found
T9495	12850-12854	IN	denotes	from
T9496	12855-12861	JJ	denotes	single
T9497	12862-12873	NNS	denotes	chromosomes
T9498	12874-12877	VBP	denotes	are
T9499	12878-12881	RB	denotes	now
T9500	12888-12894	CC	denotes	either
T9501	12895-12903	JJ	denotes	isolated
T9502	12904-12906	CC	denotes	or
T9503	12907-12917	JJ	denotes	aggregated
T9504	12918-12920	IN	denotes	in
T9505	12921-12922	DT	denotes	a
T9506	12923-12930	NN	denotes	pattern
T9507	12931-12935	RBR	denotes	less
T9508	12936-12941	JJ	denotes	dense
T9509	12942-12946	IN	denotes	than
T9510	12947-12950	DT	denotes	the
T9511	12959-12967	NNS	denotes	clusters
T9512	12951-12958	JJ	denotes	regular
T9513	12968-12976	VBN	denotes	observed
T9514	12977-12979	IN	denotes	in
T9515	12980-12987	NN	denotes	control
T9516	12988-12993	NNS	denotes	cells
T9517	12994-12995	-LRB-	denotes	(
T9518	13002-13004	NN	denotes	4B
T9519	12995-13001	NN	denotes	Figure
T9520	13004-13005	-RRB-	denotes	)
T9521	13005-13006	.	denotes	.
T9522	13006-13160	sentence	denotes	Such aggregates, which are often found at the nuclear periphery, are also revealed by DAPI staining as a typical signature of p150CAF-1 loss-of-function.
T9523	13007-13011	JJ	denotes	Such
T9524	13012-13022	NNS	denotes	aggregates
T9525	13081-13089	VBN	denotes	revealed
T9526	13022-13024	,	denotes	,
T9527	13024-13029	WDT	denotes	which
T9528	13040-13045	VBN	denotes	found
T9529	13030-13033	VBP	denotes	are
T9530	13034-13039	RB	denotes	often
T9531	13046-13048	IN	denotes	at
T9532	13049-13052	DT	denotes	the
T9533	13061-13070	NN	denotes	periphery
T9534	13053-13060	JJ	denotes	nuclear
T9535	13070-13072	,	denotes	,
T9536	13072-13075	VBP	denotes	are
T9537	13076-13080	RB	denotes	also
T9538	13090-13092	IN	denotes	by
T9539	13093-13097	NN	denotes	DAPI
T9540	13098-13106	NN	denotes	staining
T9541	13107-13109	IN	denotes	as
T9542	13110-13111	DT	denotes	a
T9543	13120-13129	NN	denotes	signature
T9544	13112-13119	JJ	denotes	typical
T9545	13130-13132	IN	denotes	of
T9546	13133-13140	NN	denotes	p150CAF
T9547	13140-13141	HYPH	denotes	-
T9548	13141-13142	CD	denotes	1
T9549	13143-13147	NN	denotes	loss
T9550	13147-13148	HYPH	denotes	-
T9551	13148-13150	IN	denotes	of
T9552	13150-13151	HYPH	denotes	-
T9553	13151-13159	NN	denotes	function
T9554	13159-13160	.	denotes	.
T9555	13160-13467	sentence	denotes	Quantification of fluorescence along a line randomly drawn across the nucleus revealed lower fluorescence intensity and a broader distribution of DAPI and major satellite hybridization signals in p150CAF-1-depleted cells (Figure 4C and 4D), indicating decondensation of pericentric heterochromatin domains.
T9556	13161-13175	NN	denotes	Quantification
T9557	13239-13247	VBD	denotes	revealed
T9558	13176-13178	IN	denotes	of
T9559	13179-13191	NN	denotes	fluorescence
T9560	13192-13197	IN	denotes	along
T9561	13198-13199	DT	denotes	a
T9562	13200-13204	NN	denotes	line
T9563	13205-13213	RB	denotes	randomly
T9564	13214-13219	VBN	denotes	drawn
T9565	13220-13226	IN	denotes	across
T9566	13227-13230	DT	denotes	the
T9567	13231-13238	NN	denotes	nucleus
T9568	13248-13253	JJR	denotes	lower
T9569	13267-13276	NN	denotes	intensity
T9570	13254-13266	NN	denotes	fluorescence
T9571	13277-13280	CC	denotes	and
T9572	13281-13282	DT	denotes	a
T9573	13291-13303	NN	denotes	distribution
T9574	13283-13290	JJR	denotes	broader
T9575	13304-13306	IN	denotes	of
T9576	13307-13311	NN	denotes	DAPI
T9577	13312-13315	CC	denotes	and
T9578	13316-13321	JJ	denotes	major
T9579	13346-13353	NNS	denotes	signals
T9580	13322-13331	NN	denotes	satellite
T9581	13332-13345	NN	denotes	hybridization
T9582	13354-13356	IN	denotes	in
T9583	13357-13364	NN	denotes	p150CAF
T9584	13367-13375	VBN	denotes	depleted
T9585	13364-13365	HYPH	denotes	-
T9586	13365-13366	CD	denotes	1
T9587	13366-13367	HYPH	denotes	-
T9588	13376-13381	NNS	denotes	cells
T9589	13382-13383	-LRB-	denotes	(
T9590	13390-13392	NN	denotes	4C
T9591	13383-13389	NN	denotes	Figure
T9592	13393-13396	CC	denotes	and
T9593	13397-13399	NN	denotes	4D
T9594	13399-13400	-RRB-	denotes	)
T9595	13400-13402	,	denotes	,
T9596	13402-13412	VBG	denotes	indicating
T9597	13413-13427	NN	denotes	decondensation
T9598	13428-13430	IN	denotes	of
T9599	13431-13442	JJ	denotes	pericentric
T9600	13459-13466	NNS	denotes	domains
T9601	13443-13458	NN	denotes	heterochromatin
T9602	13466-13467	.	denotes	.
T9603	13467-13634	sentence	denotes	In contrast, centric domains showed an intensity and shape similar to control cells, suggesting that they remain unaffected by p150CAF-1 depletion (Figure 4C and 4D).
T9604	13468-13470	IN	denotes	In
T9605	13497-13503	VBD	denotes	showed
T9606	13471-13479	NN	denotes	contrast
T9607	13479-13481	,	denotes	,
T9608	13481-13488	JJ	denotes	centric
T9609	13489-13496	NNS	denotes	domains
T9610	13504-13506	DT	denotes	an
T9611	13507-13516	NN	denotes	intensity
T9612	13517-13520	CC	denotes	and
T9613	13521-13526	NN	denotes	shape
T9614	13527-13534	JJ	denotes	similar
T9615	13535-13537	IN	denotes	to
T9616	13538-13545	NN	denotes	control
T9617	13546-13551	NNS	denotes	cells
T9618	13551-13553	,	denotes	,
T9619	13553-13563	VBG	denotes	suggesting
T9620	13564-13568	IN	denotes	that
T9621	13574-13580	VBP	denotes	remain
T9622	13569-13573	PRP	denotes	they
T9623	13581-13591	JJ	denotes	unaffected
T9624	13592-13594	IN	denotes	by
T9625	13595-13602	NN	denotes	p150CAF
T9626	13605-13614	NN	denotes	depletion
T9627	13602-13603	HYPH	denotes	-
T9628	13603-13604	CD	denotes	1
T9629	13615-13616	-LRB-	denotes	(
T9630	13623-13625	NN	denotes	4C
T9631	13616-13622	NN	denotes	Figure
T9632	13626-13629	CC	denotes	and
T9633	13630-13632	NN	denotes	4D
T9634	13632-13633	-RRB-	denotes	)
T9635	13633-13634	.	denotes	.
T9636	13634-13778	sentence	denotes	In conclusion, these results show that p150CAF-1 is required for the proper condensation and clustering of pericentric heterochromatin domains.
T9637	13635-13637	IN	denotes	In
T9638	13664-13668	VBP	denotes	show
T9639	13638-13648	NN	denotes	conclusion
T9640	13648-13650	,	denotes	,
T9641	13650-13655	DT	denotes	these
T9642	13656-13663	NNS	denotes	results
T9643	13669-13673	IN	denotes	that
T9644	13687-13695	VBN	denotes	required
T9645	13674-13681	NN	denotes	p150CAF
T9646	13681-13682	HYPH	denotes	-
T9647	13682-13683	CD	denotes	1
T9648	13684-13686	VBZ	denotes	is
T9649	13696-13699	IN	denotes	for
T9650	13700-13703	DT	denotes	the
T9651	13711-13723	NN	denotes	condensation
T9652	13704-13710	JJ	denotes	proper
T9653	13724-13727	CC	denotes	and
T9654	13728-13738	NN	denotes	clustering
T9655	13739-13741	IN	denotes	of
T9656	13742-13753	JJ	denotes	pericentric
T9657	13770-13777	NNS	denotes	domains
T9658	13754-13769	NN	denotes	heterochromatin
T9659	13777-13778	.	denotes	.
T9660	13778-15733	sentence	denotes	Figure 4 Depletion of p150CAF-1 Leads to Loss of Clustering, Altered Localization, and Decondensation of Pericentric Heterochromatin Domains Distribution of pericentric (red) and centric (green) domains was analyzed in the interphase nuclei of mouse ES cells by DNA FISH, using major satellite (pSAT) [47] and minor satellite (pMR150) [48] DNA probes, respectively. (A) In ES cells expressing control (cont) siRNA, pericentric regions from several chromosomes associate in clusters (red). These chromocenters form foci as revealed by DAPI staining (left-hand image), while centric regions (green) remain independent entities at the periphery of these domains. The right-hand image shows the merge between the pericentric and centric FISH signals. (B) The organization of pericentric domains was altered in cells expressing p150CAF-1 siRNA. Instead of forming well-defined chromocenters, pericentric domains were found either isolated or associated in heterogeneous aggregates of various sizes, often at the nuclear periphery. Scale bar = 10 μm. (C) Control ES cells. Fluorescence was quantified along a line randomly drawn across the nucleus in the merged image and data were plotted. One can distinguish clear peaks corresponding to chromocenters (red) and the condensed minor satellites (green). (D) ES cells expressing p150CAF-1 siRNA. p150CAF-1 depletion led to a lower fluorescence intensity and a broader distribution of signals corresponding to DAPI (blue) and major satellite hybridization (red, plot) while the organization of the minor satellites remained unaffected. Insets in the right-hand images show a typical chromocenter in control cells (C) and a disrupted chromocenter in p150CAF-1-depleted cells (D). Given the known role of CAF-1 in the deposition of histone H3.1 and H4 associated with DNA synthesis [11,12], we wondered whether this defect in higher-order chromatin organization could reflect an aberrant nucleosomal organization.
T9661	15501-15506	VBN	denotes	Given
T9662	15614-15622	VBD	denotes	wondered
T9663	15507-15510	DT	denotes	the
T9664	15517-15521	NN	denotes	role
T9665	15511-15516	VBN	denotes	known
T9666	15522-15524	IN	denotes	of
T9667	15525-15528	NN	denotes	CAF
T9668	15528-15529	HYPH	denotes	-
T9669	15529-15530	CD	denotes	1
T9670	15531-15533	IN	denotes	in
T9671	15534-15537	DT	denotes	the
T9672	15538-15548	NN	denotes	deposition
T9673	15549-15551	IN	denotes	of
T9674	15552-15559	NN	denotes	histone
T9675	15560-15564	NN	denotes	H3.1
T9676	15565-15568	CC	denotes	and
T9677	15569-15571	NN	denotes	H4
T9678	15572-15582	VBN	denotes	associated
T9679	15583-15587	IN	denotes	with
T9680	15588-15591	NN	denotes	DNA
T9681	15592-15601	NN	denotes	synthesis
T9682	15602-15603	-LRB-	denotes	[
T9683	15606-15608	CD	denotes	12
T9684	15603-15605	CD	denotes	11
T9685	15605-15606	,	denotes	,
T9686	15608-15609	-RRB-	denotes	]
T9687	15609-15611	,	denotes	,
T9688	15611-15613	PRP	denotes	we
T9689	15623-15630	IN	denotes	whether
T9690	15688-15695	VB	denotes	reflect
T9691	15631-15635	DT	denotes	this
T9692	15636-15642	NN	denotes	defect
T9693	15643-15645	IN	denotes	in
T9694	15646-15652	JJR	denotes	higher
T9695	15653-15658	NN	denotes	order
T9696	15652-15653	HYPH	denotes	-
T9697	15669-15681	NN	denotes	organization
T9698	15659-15668	NN	denotes	chromatin
T9699	15682-15687	MD	denotes	could
T9700	15696-15698	DT	denotes	an
T9701	15720-15732	NN	denotes	organization
T9702	15699-15707	JJ	denotes	aberrant
T9703	15708-15719	JJ	denotes	nucleosomal
T9704	15732-15733	.	denotes	.
T9705	15733-15960	sentence	denotes	Using DNase I and micrococcal nuclease (MNase) assays, we could not observe any significant difference between control and p150CAF-1-depleted ES cells at the level of bulk genome chromatin or at pericentric repeats (Figure 5).
T9706	15734-15739	VBG	denotes	Using
T9707	15802-15809	VB	denotes	observe
T9708	15740-15745	NN	denotes	DNase
T9709	15746-15747	CD	denotes	I
T9710	15748-15751	CC	denotes	and
T9711	15752-15763	JJ	denotes	micrococcal
T9712	15764-15772	NN	denotes	nuclease
T9713	15781-15787	NNS	denotes	assays
T9714	15773-15774	-LRB-	denotes	(
T9715	15774-15779	NN	denotes	MNase
T9716	15779-15780	-RRB-	denotes	)
T9717	15787-15789	,	denotes	,
T9718	15789-15791	PRP	denotes	we
T9719	15792-15797	MD	denotes	could
T9720	15798-15801	RB	denotes	not
T9721	15810-15813	DT	denotes	any
T9722	15826-15836	NN	denotes	difference
T9723	15814-15825	JJ	denotes	significant
T9724	15837-15844	IN	denotes	between
T9725	15845-15852	NN	denotes	control
T9726	15879-15884	NNS	denotes	cells
T9727	15853-15856	CC	denotes	and
T9728	15857-15864	NN	denotes	p150CAF
T9729	15867-15875	VBN	denotes	depleted
T9730	15864-15865	HYPH	denotes	-
T9731	15865-15866	CD	denotes	1
T9732	15866-15867	HYPH	denotes	-
T9733	15876-15878	NN	denotes	ES
T9734	15885-15887	IN	denotes	at
T9735	15888-15891	DT	denotes	the
T9736	15892-15897	NN	denotes	level
T9737	15898-15900	IN	denotes	of
T9738	15901-15905	NN	denotes	bulk
T9739	15913-15922	NN	denotes	chromatin
T9740	15906-15912	NN	denotes	genome
T9741	15923-15925	CC	denotes	or
T9742	15926-15928	IN	denotes	at
T9743	15929-15940	JJ	denotes	pericentric
T9744	15941-15948	NNS	denotes	repeats
T9745	15949-15950	-LRB-	denotes	(
T9746	15950-15956	NN	denotes	Figure
T9747	15957-15958	CD	denotes	5
T9748	15958-15959	-RRB-	denotes	)
T9749	15959-15960	.	denotes	.
T9750	15960-16143	sentence	denotes	In a second series of experiments, we compared the association of histone H3 with chromatin in nuclei isolated from cells transfected with control and p150CAF-1 RNAi plasmid vectors.
T9751	15961-15963	IN	denotes	In
T9752	15999-16007	VBD	denotes	compared
T9753	15964-15965	DT	denotes	a
T9754	15973-15979	NN	denotes	series
T9755	15966-15972	JJ	denotes	second
T9756	15980-15982	IN	denotes	of
T9757	15983-15994	NNS	denotes	experiments
T9758	15994-15996	,	denotes	,
T9759	15996-15998	PRP	denotes	we
T9760	16008-16011	DT	denotes	the
T9761	16012-16023	NN	denotes	association
T9762	16024-16026	IN	denotes	of
T9763	16027-16034	NN	denotes	histone
T9764	16035-16037	NN	denotes	H3
T9765	16038-16042	IN	denotes	with
T9766	16043-16052	NN	denotes	chromatin
T9767	16053-16055	IN	denotes	in
T9768	16056-16062	NNS	denotes	nuclei
T9769	16063-16071	VBN	denotes	isolated
T9770	16072-16076	IN	denotes	from
T9771	16077-16082	NNS	denotes	cells
T9772	16083-16094	VBN	denotes	transfected
T9773	16095-16099	IN	denotes	with
T9774	16100-16107	NN	denotes	control
T9775	16135-16142	NNS	denotes	vectors
T9776	16108-16111	CC	denotes	and
T9777	16112-16119	NN	denotes	p150CAF
T9778	16119-16120	HYPH	denotes	-
T9779	16120-16121	CD	denotes	1
T9780	16122-16126	NN	denotes	RNAi
T9781	16127-16134	NN	denotes	plasmid
T9782	16142-16143	.	denotes	.
T9783	16143-16249	sentence	denotes	Nuclei were incubated in buffers with different salt concentrations ranging from 100 mM NaCl to 1 M NaCl.
T9784	16144-16150	NNS	denotes	Nuclei
T9785	16156-16165	VBN	denotes	incubated
T9786	16151-16155	VBD	denotes	were
T9787	16166-16168	IN	denotes	in
T9788	16169-16176	NNS	denotes	buffers
T9789	16177-16181	IN	denotes	with
T9790	16182-16191	JJ	denotes	different
T9791	16197-16211	NNS	denotes	concentrations
T9792	16192-16196	NN	denotes	salt
T9793	16212-16219	VBG	denotes	ranging
T9794	16220-16224	IN	denotes	from
T9795	16225-16228	CD	denotes	100
T9796	16229-16231	NN	denotes	mM
T9797	16232-16236	NN	denotes	NaCl
T9798	16237-16239	IN	denotes	to
T9799	16240-16241	CD	denotes	1
T9800	16242-16243	NN	denotes	M
T9801	16244-16248	NN	denotes	NaCl
T9802	16248-16249	.	denotes	.
T9803	16249-16414	sentence	denotes	In all conditions, the amount of histone H3 remaining associated with chromatin was indistinguishable in control and p150CAF-1-depleted ES cells (unpublished data).
T9804	16250-16252	IN	denotes	In
T9805	16330-16333	VBD	denotes	was
T9806	16253-16256	DT	denotes	all
T9807	16257-16267	NNS	denotes	conditions
T9808	16267-16269	,	denotes	,
T9809	16269-16272	DT	denotes	the
T9810	16273-16279	NN	denotes	amount
T9811	16280-16282	IN	denotes	of
T9812	16283-16290	NN	denotes	histone
T9813	16291-16293	NN	denotes	H3
T9814	16294-16303	VBG	denotes	remaining
T9815	16304-16314	VBN	denotes	associated
T9816	16315-16319	IN	denotes	with
T9817	16320-16329	NN	denotes	chromatin
T9818	16334-16351	JJ	denotes	indistinguishable
T9819	16352-16354	IN	denotes	in
T9820	16355-16362	NN	denotes	control
T9821	16389-16394	NNS	denotes	cells
T9822	16363-16366	CC	denotes	and
T9823	16367-16374	NN	denotes	p150CAF
T9824	16377-16385	VBN	denotes	depleted
T9825	16374-16375	HYPH	denotes	-
T9826	16375-16376	CD	denotes	1
T9827	16376-16377	HYPH	denotes	-
T9828	16386-16388	NN	denotes	ES
T9829	16395-16396	-LRB-	denotes	(
T9830	16408-16412	NNS	denotes	data
T9831	16396-16407	JJ	denotes	unpublished
T9832	16412-16413	-RRB-	denotes	)
T9833	16413-16414	.	denotes	.
T9834	16414-16580	sentence	denotes	Therefore, the loss of clustering and decondensation of pericentric heterochromatin are unlikely to be the consequence of severe defects in nucleosomal organization.
T9835	16415-16424	RB	denotes	Therefore
T9836	16499-16502	VBP	denotes	are
T9837	16424-16426	,	denotes	,
T9838	16426-16429	DT	denotes	the
T9839	16430-16434	NN	denotes	loss
T9840	16435-16437	IN	denotes	of
T9841	16438-16448	NN	denotes	clustering
T9842	16449-16452	CC	denotes	and
T9843	16453-16467	NN	denotes	decondensation
T9844	16468-16470	IN	denotes	of
T9845	16471-16482	JJ	denotes	pericentric
T9846	16483-16498	NN	denotes	heterochromatin
T9847	16503-16511	JJ	denotes	unlikely
T9848	16512-16514	TO	denotes	to
T9849	16515-16517	VB	denotes	be
T9850	16518-16521	DT	denotes	the
T9851	16522-16533	NN	denotes	consequence
T9852	16534-16536	IN	denotes	of
T9853	16537-16543	JJ	denotes	severe
T9854	16544-16551	NNS	denotes	defects
T9855	16552-16554	IN	denotes	in
T9856	16555-16566	JJ	denotes	nucleosomal
T9857	16567-16579	NN	denotes	organization
T9858	16579-16580	.	denotes	.
T9859	16580-17252	sentence	denotes	Figure 5 Nucleosomal Organization Is Not Altered in p150CAF-1-Depleted ES Cells Nuclei were prepared from ES cells transfected with control or p150CAF-1 siRNA vector. Nuclei were digested with increasing amounts of DNase I or MNase. (A) After digestion with the indicated nucleases, total DNA was prepared and run onto an agarose gel which was stained with ethidium bromide to reveal bulk genomic DNA. (B) The DNA was blotted onto a nylon membrane, which was then hybridized with the α-32P-labeled pSAT major satellite repeat probe [47]. Our findings showed that the nuclear localization of HP1α is severely altered in Chaf1a −/− embryos and p150CAF-1-depleted ES cells.
T9860	17120-17123	PRP$	denotes	Our
T9861	17124-17132	NNS	denotes	findings
T9862	17133-17139	VBD	denotes	showed
T9863	17140-17144	IN	denotes	that
T9864	17190-17197	VBN	denotes	altered
T9865	17145-17148	DT	denotes	the
T9866	17157-17169	NN	denotes	localization
T9867	17149-17156	JJ	denotes	nuclear
T9868	17170-17172	IN	denotes	of
T9869	17173-17177	NN	denotes	HP1α
T9870	17178-17180	VBZ	denotes	is
T9871	17181-17189	RB	denotes	severely
T9872	17198-17200	IN	denotes	in
T9873	17201-17207	NN	denotes	Chaf1a
T9874	17212-17219	NNS	denotes	embryos
T9875	17208-17209	SYM	denotes	−
T9876	17209-17210	HYPH	denotes	/
T9877	17210-17211	SYM	denotes	−
T9878	17220-17223	CC	denotes	and
T9879	17224-17231	NN	denotes	p150CAF
T9880	17234-17242	VBN	denotes	depleted
T9881	17231-17232	HYPH	denotes	-
T9882	17232-17233	CD	denotes	1
T9883	17233-17234	HYPH	denotes	-
T9884	17246-17251	NNS	denotes	cells
T9885	17243-17245	NN	denotes	ES
T9886	17251-17252	.	denotes	.
T9887	17252-17373	sentence	denotes	We therefore examined the status of other epigenetic marks previously shown to characterize pericentric heterochromatin.
T9888	17253-17255	PRP	denotes	We
T9889	17266-17274	VBD	denotes	examined
T9890	17256-17265	RB	denotes	therefore
T9891	17275-17278	DT	denotes	the
T9892	17279-17285	NN	denotes	status
T9893	17286-17288	IN	denotes	of
T9894	17289-17294	JJ	denotes	other
T9895	17306-17311	NNS	denotes	marks
T9896	17295-17305	JJ	denotes	epigenetic
T9897	17312-17322	RB	denotes	previously
T9898	17323-17328	VBN	denotes	shown
T9899	17329-17331	TO	denotes	to
T9900	17332-17344	VB	denotes	characterize
T9901	17345-17356	JJ	denotes	pericentric
T9902	17357-17372	NN	denotes	heterochromatin
T9903	17372-17373	.	denotes	.
T9904	17373-17534	sentence	denotes	We first tested DNA CpG methylation [25] at major satellite repeats and found no significant difference between control and p150CAF-1-depleted cells (Figure 6).
T9905	17374-17376	PRP	denotes	We
T9906	17383-17389	VBD	denotes	tested
T9907	17377-17382	RB	denotes	first
T9908	17390-17393	NN	denotes	DNA
T9909	17398-17409	NN	denotes	methylation
T9910	17394-17397	NN	denotes	CpG
T9911	17410-17411	-LRB-	denotes	[
T9912	17411-17413	CD	denotes	25
T9913	17413-17414	-RRB-	denotes	]
T9914	17415-17417	IN	denotes	at
T9915	17418-17423	JJ	denotes	major
T9916	17434-17441	NNS	denotes	repeats
T9917	17424-17433	NN	denotes	satellite
T9918	17442-17445	CC	denotes	and
T9919	17446-17451	VBD	denotes	found
T9920	17452-17454	DT	denotes	no
T9921	17467-17477	NN	denotes	difference
T9922	17455-17466	JJ	denotes	significant
T9923	17478-17485	IN	denotes	between
T9924	17486-17493	NN	denotes	control
T9925	17517-17522	NNS	denotes	cells
T9926	17494-17497	CC	denotes	and
T9927	17498-17505	NN	denotes	p150CAF
T9928	17508-17516	VBN	denotes	depleted
T9929	17505-17506	HYPH	denotes	-
T9930	17506-17507	CD	denotes	1
T9931	17507-17508	HYPH	denotes	-
T9932	17523-17524	-LRB-	denotes	(
T9933	17524-17530	NN	denotes	Figure
T9934	17531-17532	CD	denotes	6
T9935	17532-17533	-RRB-	denotes	)
T9936	17533-17534	.	denotes	.
T9937	17534-17681	sentence	denotes	As the LTM7 ES cell line that we used in our RNAi experiments is a female (XX) cell line, DNA methylation is globally reduced in these cells [26].
T9938	17535-17537	IN	denotes	As
T9939	17597-17599	VBZ	denotes	is
T9940	17538-17541	DT	denotes	the
T9941	17555-17559	NN	denotes	line
T9942	17542-17546	NN	denotes	LTM7
T9943	17547-17549	NN	denotes	ES
T9944	17550-17554	NN	denotes	cell
T9945	17560-17564	WDT	denotes	that
T9946	17568-17572	VBD	denotes	used
T9947	17565-17567	PRP	denotes	we
T9948	17573-17575	IN	denotes	in
T9949	17576-17579	PRP$	denotes	our
T9950	17585-17596	NNS	denotes	experiments
T9951	17580-17584	NN	denotes	RNAi
T9952	17653-17660	VBN	denotes	reduced
T9953	17600-17601	DT	denotes	a
T9954	17619-17623	NN	denotes	line
T9955	17602-17608	JJ	denotes	female
T9956	17609-17610	-LRB-	denotes	(
T9957	17610-17612	NN	denotes	XX
T9958	17612-17613	-RRB-	denotes	)
T9959	17614-17618	NN	denotes	cell
T9960	17623-17625	,	denotes	,
T9961	17625-17628	NN	denotes	DNA
T9962	17629-17640	NN	denotes	methylation
T9963	17641-17643	VBZ	denotes	is
T9964	17644-17652	RB	denotes	globally
T9965	17661-17663	IN	denotes	in
T9966	17664-17669	DT	denotes	these
T9967	17670-17675	NNS	denotes	cells
T9968	17676-17677	-LRB-	denotes	[
T9969	17677-17679	CD	denotes	26
T9970	17679-17680	-RRB-	denotes	]
T9971	17680-17681	.	denotes	.
T9972	17681-18087	sentence	denotes	To rule out the possibility that hypomethylation of pericentric repeats might contribute to the destabilization of heterochromatin domains in p150CAF-1-depleted ES cells, we tested our RNAi plasmid vector in a XY ES cell line, and confirmed that loss of p150CAF-1 leads to disruption of heterochromatin organization independently of the degree of DNA methylation at pericentric repeats (unpublished data).
T9973	17682-17684	TO	denotes	To
T9974	17685-17689	VB	denotes	rule
T9975	17856-17862	VBD	denotes	tested
T9976	17690-17693	RP	denotes	out
T9977	17694-17697	DT	denotes	the
T9978	17698-17709	NN	denotes	possibility
T9979	17710-17714	IN	denotes	that
T9980	17760-17770	VB	denotes	contribute
T9981	17715-17730	NN	denotes	hypomethylation
T9982	17731-17733	IN	denotes	of
T9983	17734-17745	JJ	denotes	pericentric
T9984	17746-17753	NNS	denotes	repeats
T9985	17754-17759	MD	denotes	might
T9986	17771-17773	IN	denotes	to
T9987	17774-17777	DT	denotes	the
T9988	17778-17793	NN	denotes	destabilization
T9989	17794-17796	IN	denotes	of
T9990	17797-17812	NN	denotes	heterochromatin
T9991	17813-17820	NNS	denotes	domains
T9992	17821-17823	IN	denotes	in
T9993	17824-17831	NN	denotes	p150CAF
T9994	17834-17842	VBN	denotes	depleted
T9995	17831-17832	HYPH	denotes	-
T9996	17832-17833	CD	denotes	1
T9997	17833-17834	HYPH	denotes	-
T9998	17846-17851	NNS	denotes	cells
T9999	17843-17845	NN	denotes	ES
T10000	17851-17853	,	denotes	,
T10001	17853-17855	PRP	denotes	we
T10002	17863-17866	PRP$	denotes	our
T10003	17880-17886	NN	denotes	vector
T10004	17867-17871	NN	denotes	RNAi
T10005	17872-17879	NN	denotes	plasmid
T10006	17887-17889	IN	denotes	in
T10007	17890-17891	DT	denotes	a
T10008	17903-17907	NN	denotes	line
T10009	17892-17894	NN	denotes	XY
T10010	17895-17897	NN	denotes	ES
T10011	17898-17902	NN	denotes	cell
T10012	17907-17909	,	denotes	,
T10013	17909-17912	CC	denotes	and
T10014	17913-17922	VBD	denotes	confirmed
T10015	17923-17927	IN	denotes	that
T10016	17946-17951	VBZ	denotes	leads
T10017	17928-17932	NN	denotes	loss
T10018	17933-17935	IN	denotes	of
T10019	17936-17943	NN	denotes	p150CAF
T10020	17943-17944	HYPH	denotes	-
T10021	17944-17945	CD	denotes	1
T10022	17952-17954	IN	denotes	to
T10023	17955-17965	NN	denotes	disruption
T10024	17966-17968	IN	denotes	of
T10025	17969-17984	NN	denotes	heterochromatin
T10026	17985-17997	NN	denotes	organization
T10027	17998-18011	RB	denotes	independently
T10028	18012-18014	IN	denotes	of
T10029	18015-18018	DT	denotes	the
T10030	18019-18025	NN	denotes	degree
T10031	18026-18028	IN	denotes	of
T10032	18029-18032	NN	denotes	DNA
T10033	18033-18044	NN	denotes	methylation
T10034	18045-18047	IN	denotes	at
T10035	18048-18059	JJ	denotes	pericentric
T10036	18060-18067	NNS	denotes	repeats
T10037	18068-18069	-LRB-	denotes	(
T10038	18081-18085	NNS	denotes	data
T10039	18069-18080	JJ	denotes	unpublished
T10040	18085-18086	-RRB-	denotes	)
T10041	18086-18087	.	denotes	.
T10042	18087-18221	sentence	denotes	We next analyzed specific histone modifications [27] in chromatin immunoprecipitation (ChIP) experiments using native chromatin [28].
T10043	18088-18090	PRP	denotes	We
T10044	18096-18104	VBD	denotes	analyzed
T10045	18091-18095	RB	denotes	next
T10046	18105-18113	JJ	denotes	specific
T10047	18122-18135	NNS	denotes	modifications
T10048	18114-18121	NN	denotes	histone
T10049	18136-18137	-LRB-	denotes	[
T10050	18137-18139	CD	denotes	27
T10051	18139-18140	-RRB-	denotes	]
T10052	18141-18143	IN	denotes	in
T10053	18144-18153	NN	denotes	chromatin
T10054	18154-18173	NN	denotes	immunoprecipitation
T10055	18181-18192	NNS	denotes	experiments
T10056	18174-18175	-LRB-	denotes	(
T10057	18175-18179	NN	denotes	ChIP
T10058	18179-18180	-RRB-	denotes	)
T10059	18193-18198	VBG	denotes	using
T10060	18199-18205	JJ	denotes	native
T10061	18206-18215	NN	denotes	chromatin
T10062	18216-18217	-LRB-	denotes	[
T10063	18217-18219	CD	denotes	28
T10064	18219-18220	-RRB-	denotes	]
T10065	18220-18221	.	denotes	.
T10066	18221-18388	sentence	denotes	We found that pericentric DNA was immunoprecipitated with a 2-fold lower efficiency with H4K20me3 [29,30] antibodies following p150CAF-1 depletion (Figure 7A and 7B).
T10067	18222-18224	PRP	denotes	We
T10068	18225-18230	VBD	denotes	found
T10069	18231-18235	IN	denotes	that
T10070	18256-18274	VBN	denotes	immunoprecipitated
T10071	18236-18247	JJ	denotes	pericentric
T10072	18248-18251	NN	denotes	DNA
T10073	18252-18255	VBD	denotes	was
T10074	18275-18279	IN	denotes	with
T10075	18280-18281	DT	denotes	a
T10076	18295-18305	NN	denotes	efficiency
T10077	18282-18283	CD	denotes	2
T10078	18289-18294	JJR	denotes	lower
T10079	18283-18284	HYPH	denotes	-
T10080	18284-18288	RB	denotes	fold
T10081	18306-18310	IN	denotes	with
T10082	18311-18319	NN	denotes	H4K20me3
T10083	18328-18338	NNS	denotes	antibodies
T10084	18320-18321	-LRB-	denotes	[
T10085	18324-18326	CD	denotes	30
T10086	18321-18323	CD	denotes	29
T10087	18323-18324	,	denotes	,
T10088	18326-18327	-RRB-	denotes	]
T10089	18339-18348	VBG	denotes	following
T10090	18349-18356	NN	denotes	p150CAF
T10091	18359-18368	NN	denotes	depletion
T10092	18356-18357	HYPH	denotes	-
T10093	18357-18358	CD	denotes	1
T10094	18369-18370	-LRB-	denotes	(
T10095	18377-18379	NN	denotes	7A
T10096	18370-18376	NN	denotes	Figure
T10097	18380-18383	CC	denotes	and
T10098	18384-18386	NN	denotes	7B
T10099	18386-18387	-RRB-	denotes	)
T10100	18387-18388	.	denotes	.
T10101	18388-18585	sentence	denotes	Immunoprecipitation of minor satellite repeats in the same experiment was less affected (Figure 7B), showing that loss of p150CAF-1 function mainly affects H4K20me3 at pericentric heterochromatin.
T10102	18389-18408	NN	denotes	Immunoprecipitation
T10103	18468-18476	VBN	denotes	affected
T10104	18409-18411	IN	denotes	of
T10105	18412-18417	JJ	denotes	minor
T10106	18428-18435	NNS	denotes	repeats
T10107	18418-18427	NN	denotes	satellite
T10108	18436-18438	IN	denotes	in
T10109	18439-18442	DT	denotes	the
T10110	18448-18458	NN	denotes	experiment
T10111	18443-18447	JJ	denotes	same
T10112	18459-18462	VBD	denotes	was
T10113	18463-18467	RBR	denotes	less
T10114	18477-18478	-LRB-	denotes	(
T10115	18485-18487	NN	denotes	7B
T10116	18478-18484	NN	denotes	Figure
T10117	18487-18488	-RRB-	denotes	)
T10118	18488-18490	,	denotes	,
T10119	18490-18497	VBG	denotes	showing
T10120	18498-18502	IN	denotes	that
T10121	18537-18544	VBZ	denotes	affects
T10122	18503-18507	NN	denotes	loss
T10123	18508-18510	IN	denotes	of
T10124	18511-18518	NN	denotes	p150CAF
T10125	18521-18529	NN	denotes	function
T10126	18518-18519	HYPH	denotes	-
T10127	18519-18520	CD	denotes	1
T10128	18530-18536	RB	denotes	mainly
T10129	18545-18553	NN	denotes	H4K20me3
T10130	18554-18556	IN	denotes	at
T10131	18557-18568	JJ	denotes	pericentric
T10132	18569-18584	NN	denotes	heterochromatin
T10133	18584-18585	.	denotes	.
T10134	18585-18804	sentence	denotes	Moreover, no significant difference in immunoprecipitation efficiency was detected at intracisternal A particle (IAP) elements (Figure 7B), which are noncentromeric repeated DNA elements also enriched in H4K20me3 [31].
T10135	18586-18594	RB	denotes	Moreover
T10136	18660-18668	VBN	denotes	detected
T10137	18594-18596	,	denotes	,
T10138	18596-18598	DT	denotes	no
T10139	18611-18621	NN	denotes	difference
T10140	18599-18610	JJ	denotes	significant
T10141	18622-18624	IN	denotes	in
T10142	18625-18644	NN	denotes	immunoprecipitation
T10143	18645-18655	NN	denotes	efficiency
T10144	18656-18659	VBD	denotes	was
T10145	18669-18671	IN	denotes	at
T10146	18672-18686	JJ	denotes	intracisternal
T10147	18689-18697	NN	denotes	particle
T10148	18687-18688	NN	denotes	A
T10149	18704-18712	NNS	denotes	elements
T10150	18698-18699	-LRB-	denotes	(
T10151	18699-18702	NN	denotes	IAP
T10152	18702-18703	-RRB-	denotes	)
T10153	18713-18714	-LRB-	denotes	(
T10154	18721-18723	NN	denotes	7B
T10155	18714-18720	NN	denotes	Figure
T10156	18723-18724	-RRB-	denotes	)
T10157	18724-18726	,	denotes	,
T10158	18726-18731	WDT	denotes	which
T10159	18732-18735	VBP	denotes	are
T10160	18736-18750	JJ	denotes	noncentromeric
T10161	18764-18772	NNS	denotes	elements
T10162	18751-18759	VBN	denotes	repeated
T10163	18760-18763	NN	denotes	DNA
T10164	18773-18777	RB	denotes	also
T10165	18778-18786	VBN	denotes	enriched
T10166	18787-18789	IN	denotes	in
T10167	18790-18798	NN	denotes	H4K20me3
T10168	18799-18800	-LRB-	denotes	[
T10169	18800-18802	CD	denotes	31
T10170	18802-18803	-RRB-	denotes	]
T10171	18803-18804	.	denotes	.
T10172	18804-18945	sentence	denotes	This result shows that p150CAF-1 contributes to normal levels of H4K20me3 at pericentric heterochromatin, but not necessarily at other loci.
T10173	18805-18809	DT	denotes	This
T10174	18810-18816	NN	denotes	result
T10175	18817-18822	VBZ	denotes	shows
T10176	18823-18827	IN	denotes	that
T10177	18838-18849	VBZ	denotes	contributes
T10178	18828-18835	NN	denotes	p150CAF
T10179	18835-18836	HYPH	denotes	-
T10180	18836-18837	CD	denotes	1
T10181	18850-18852	IN	denotes	to
T10182	18853-18859	JJ	denotes	normal
T10183	18860-18866	NNS	denotes	levels
T10184	18867-18869	IN	denotes	of
T10185	18870-18878	NN	denotes	H4K20me3
T10186	18879-18881	IN	denotes	at
T10187	18882-18893	JJ	denotes	pericentric
T10188	18894-18909	NN	denotes	heterochromatin
T10189	18909-18911	,	denotes	,
T10190	18911-18914	CC	denotes	but
T10191	18915-18918	RB	denotes	not
T10192	18919-18930	RB	denotes	necessarily
T10193	18931-18933	IN	denotes	at
T10194	18934-18939	JJ	denotes	other
T10195	18940-18944	NNS	denotes	loci
T10196	18944-18945	.	denotes	.
T10197	18945-19087	sentence	denotes	The H3K9me3 mark [32] was also significantly reduced in p150CAF-1-depleted cells, though to a lesser extent than H4K20me3 (Figure 7A and 7B).
T10198	18946-18949	DT	denotes	The
T10199	18958-18962	NN	denotes	mark
T10200	18950-18957	NN	denotes	H3K9me3
T10201	18991-18998	VBN	denotes	reduced
T10202	18963-18964	-LRB-	denotes	[
T10203	18964-18966	CD	denotes	32
T10204	18966-18967	-RRB-	denotes	]
T10205	18968-18971	VBD	denotes	was
T10206	18972-18976	RB	denotes	also
T10207	18977-18990	RB	denotes	significantly
T10208	18999-19001	IN	denotes	in
T10209	19002-19009	NN	denotes	p150CAF
T10210	19012-19020	VBN	denotes	depleted
T10211	19009-19010	HYPH	denotes	-
T10212	19010-19011	CD	denotes	1
T10213	19011-19012	HYPH	denotes	-
T10214	19021-19026	NNS	denotes	cells
T10215	19026-19028	,	denotes	,
T10216	19028-19034	IN	denotes	though
T10217	19035-19037	IN	denotes	to
T10218	19038-19039	DT	denotes	a
T10219	19047-19053	NN	denotes	extent
T10220	19040-19046	JJR	denotes	lesser
T10221	19054-19058	IN	denotes	than
T10222	19059-19067	NN	denotes	H4K20me3
T10223	19068-19069	-LRB-	denotes	(
T10224	19076-19078	NN	denotes	7A
T10225	19069-19075	NN	denotes	Figure
T10226	19079-19082	CC	denotes	and
T10227	19083-19085	NN	denotes	7B
T10228	19085-19086	-RRB-	denotes	)
T10229	19086-19087	.	denotes	.
T10230	19087-20985	sentence	denotes	Figure 6 DNA CpG Methylation at Pericentric Heterochromatin Is Not Altered in p150CAF-1-Depleted ES Cells Total DNA was isolated from cells transfected with a control or the p150CAF-1 siRNA vector and digested with MaeII, whose recognition sequence is present in major satellite repeats. Digested DNA was run onto an agarose gel and analyzed by Southern blotting using the pSAT major satellite probe [47]. Figure 7 Alteration of Epigenetic Marking at Pericentric Heterochromatin in p150CAF-1-Depleted Cells (A) p150CAF-1 depletion leads to reduced H4K20me3 and H3K9me3 at pericentric heterochromatin. Enrichment of histone marks at major satellite repeats was determined by ChIP from control (cont) and p150CAF-1 (p150) siRNA-expressing ES cells. DNA prepared from the input and the antibody-bound fraction were run onto an agarose gel and analyzed by Southern blot with the pSAT major satellite repeat probe [47]. (B) Hybridization signals were quantified using an Instant Imager. After autoradiography, the membrane was stripped and rehybridized with a minor satellite probe [49]. After quantification, the membrane was stripped and rehybridized with an IAP LTR probe [50]. Results are presented as the amount of DNA immunoprecipitated from p150CAF-1-depleted ES cells divided by the DNA obtained from control cells. The figure shows the mean value and standard deviation of three independent ChIP experiments. (C and D) H3K9me3 and H4K20me3 fluorescence patterns are severely altered in p150CAF-1-depleted ES cells. Immunodetection of H3K9me3 (C, green), H4K20me3 (D, green), and HP1α (red) in control and p150CAF-1 siRNA-expressing ES cells. Merging of HP1α with H3K9me3 (C) and H4K20me3 (D) is shown in yellow. Scale bars represent 10 μm. In wild-type cells, most of H4K20me3 and H3K9me3 signals are present at the level of DAPI-dense domains when visualized by IF [29] (Figures 7C and 7D).
T10231	20834-20836	IN	denotes	In
T10232	20891-20894	VBP	denotes	are
T10233	20837-20841	JJ	denotes	wild
T10234	20842-20846	NN	denotes	type
T10235	20841-20842	HYPH	denotes	-
T10236	20847-20852	NNS	denotes	cells
T10237	20852-20854	,	denotes	,
T10238	20854-20858	JJS	denotes	most
T10239	20859-20861	IN	denotes	of
T10240	20862-20870	NN	denotes	H4K20me3
T10241	20883-20890	NNS	denotes	signals
T10242	20871-20874	CC	denotes	and
T10243	20875-20882	NN	denotes	H3K9me3
T10244	20895-20902	JJ	denotes	present
T10245	20903-20905	IN	denotes	at
T10246	20906-20909	DT	denotes	the
T10247	20910-20915	NN	denotes	level
T10248	20916-20918	IN	denotes	of
T10249	20919-20923	NN	denotes	DAPI
T10250	20924-20929	JJ	denotes	dense
T10251	20923-20924	HYPH	denotes	-
T10252	20930-20937	NNS	denotes	domains
T10253	20938-20942	WRB	denotes	when
T10254	20943-20953	VBN	denotes	visualized
T10255	20954-20956	IN	denotes	by
T10256	20957-20959	NN	denotes	IF
T10257	20960-20961	-LRB-	denotes	[
T10258	20961-20963	CD	denotes	29
T10259	20963-20964	-RRB-	denotes	]
T10260	20965-20966	-LRB-	denotes	(
T10261	20974-20976	NN	denotes	7C
T10262	20966-20973	NNS	denotes	Figures
T10263	20977-20980	CC	denotes	and
T10264	20981-20983	NN	denotes	7D
T10265	20983-20984	-RRB-	denotes	)
T10266	20984-20985	.	denotes	.
T10267	20985-21103	sentence	denotes	H3K9me3 was severely perturbed in p150CAF-1-depleted cells, displaying a diffuse pattern similar to HP1α (Figure 7C).
T10268	20986-20993	NN	denotes	H3K9me3
T10269	21007-21016	VBN	denotes	perturbed
T10270	20994-20997	VBD	denotes	was
T10271	20998-21006	RB	denotes	severely
T10272	21017-21019	IN	denotes	in
T10273	21020-21027	NN	denotes	p150CAF
T10274	21030-21038	VBN	denotes	depleted
T10275	21027-21028	HYPH	denotes	-
T10276	21028-21029	CD	denotes	1
T10277	21029-21030	HYPH	denotes	-
T10278	21039-21044	NNS	denotes	cells
T10279	21044-21046	,	denotes	,
T10280	21046-21056	VBG	denotes	displaying
T10281	21057-21058	DT	denotes	a
T10282	21067-21074	NN	denotes	pattern
T10283	21059-21066	JJ	denotes	diffuse
T10284	21075-21082	JJ	denotes	similar
T10285	21083-21085	IN	denotes	to
T10286	21086-21090	NN	denotes	HP1α
T10287	21091-21092	-LRB-	denotes	(
T10288	21099-21101	NN	denotes	7C
T10289	21092-21098	NN	denotes	Figure
T10290	21101-21102	-RRB-	denotes	)
T10291	21102-21103	.	denotes	.
T10292	21103-21271	sentence	denotes	The H4K20me3 pattern was even more severely altered, showing both a loss of the typical dots revealed in control cells and a reduction in signal intensity (Figure 7D).
T10293	21104-21107	DT	denotes	The
T10294	21117-21124	NN	denotes	pattern
T10295	21108-21116	NN	denotes	H4K20me3
T10296	21148-21155	VBN	denotes	altered
T10297	21125-21128	VBD	denotes	was
T10298	21129-21133	RB	denotes	even
T10299	21134-21138	RBR	denotes	more
T10300	21139-21147	RB	denotes	severely
T10301	21155-21157	,	denotes	,
T10302	21157-21164	VBG	denotes	showing
T10303	21165-21169	CC	denotes	both
T10304	21172-21176	NN	denotes	loss
T10305	21170-21171	DT	denotes	a
T10306	21177-21179	IN	denotes	of
T10307	21180-21183	DT	denotes	the
T10308	21192-21196	NNS	denotes	dots
T10309	21184-21191	JJ	denotes	typical
T10310	21197-21205	VBN	denotes	revealed
T10311	21206-21208	IN	denotes	in
T10312	21209-21216	NN	denotes	control
T10313	21217-21222	NNS	denotes	cells
T10314	21223-21226	CC	denotes	and
T10315	21227-21228	DT	denotes	a
T10316	21229-21238	NN	denotes	reduction
T10317	21239-21241	IN	denotes	in
T10318	21242-21248	NN	denotes	signal
T10319	21249-21258	NN	denotes	intensity
T10320	21259-21260	-LRB-	denotes	(
T10321	21267-21269	NN	denotes	7D
T10322	21260-21266	NN	denotes	Figure
T10323	21269-21270	-RRB-	denotes	)
T10324	21270-21271	.	denotes	.
T10325	21271-21397	sentence	denotes	Consistent with our ChIP experiments, residual H4K20me3 epitopes were detected at the level of relocated DAPI-dense material.
T10326	21272-21282	JJ	denotes	Consistent
T10327	21342-21350	VBN	denotes	detected
T10328	21283-21287	IN	denotes	with
T10329	21288-21291	PRP$	denotes	our
T10330	21297-21308	NNS	denotes	experiments
T10331	21292-21296	NN	denotes	ChIP
T10332	21308-21310	,	denotes	,
T10333	21310-21318	JJ	denotes	residual
T10334	21328-21336	NNS	denotes	epitopes
T10335	21319-21327	NN	denotes	H4K20me3
T10336	21337-21341	VBD	denotes	were
T10337	21351-21353	IN	denotes	at
T10338	21354-21357	DT	denotes	the
T10339	21358-21363	NN	denotes	level
T10340	21364-21366	IN	denotes	of
T10341	21367-21376	VBN	denotes	relocated
T10342	21388-21396	NN	denotes	material
T10343	21377-21381	NN	denotes	DAPI
T10344	21382-21387	JJ	denotes	dense
T10345	21381-21382	HYPH	denotes	-
T10346	21396-21397	.	denotes	.
T10347	21397-21574	sentence	denotes	Altogether, our results show that depletion of CAF-1 in ES cells leads to a simultaneous disruption of heterochromatin 3-D organization and an alteration in epigenetic marking.
T10348	21398-21408	RB	denotes	Altogether
T10349	21422-21426	VBP	denotes	show
T10350	21408-21410	,	denotes	,
T10351	21410-21413	PRP$	denotes	our
T10352	21414-21421	NNS	denotes	results
T10353	21427-21431	IN	denotes	that
T10354	21463-21468	VBZ	denotes	leads
T10355	21432-21441	NN	denotes	depletion
T10356	21442-21444	IN	denotes	of
T10357	21445-21448	NN	denotes	CAF
T10358	21448-21449	HYPH	denotes	-
T10359	21449-21450	CD	denotes	1
T10360	21451-21453	IN	denotes	in
T10361	21454-21456	NN	denotes	ES
T10362	21457-21462	NNS	denotes	cells
T10363	21469-21471	IN	denotes	to
T10364	21472-21473	DT	denotes	a
T10365	21487-21497	NN	denotes	disruption
T10366	21474-21486	JJ	denotes	simultaneous
T10367	21498-21500	IN	denotes	of
T10368	21501-21516	NN	denotes	heterochromatin
T10369	21521-21533	NN	denotes	organization
T10370	21517-21518	CD	denotes	3
T10371	21519-21520	NN	denotes	D
T10372	21518-21519	HYPH	denotes	-
T10373	21534-21537	CC	denotes	and
T10374	21538-21540	DT	denotes	an
T10375	21541-21551	NN	denotes	alteration
T10376	21552-21554	IN	denotes	in
T10377	21555-21565	JJ	denotes	epigenetic
T10378	21566-21573	NN	denotes	marking
T10379	21573-21574	.	denotes	.
T8538	5453-5455	CD	denotes	16
R795	T7971	T7972	nsubj	We,used
R805	T7973	T7974	compound	gene,targeting
R806	T7974	T7972	dobj	targeting,used
R807	T7975	T7972	prep	in,used
R808	T7976	T7977	compound	ES,cells
R809	T7977	T7975	pobj	cells,in
R810	T7978	T7979	aux	to,delete
R811	T8011	T8010	prep	of,were
R812	T7979	T7972	advcl	delete,used
R813	T8012	T8013	amod	normal,size
R814	T8013	T8011	pobj	size,of
R815	T8014	T8013	cc	and,size
R816	T8015	T8013	conj	weight,size
R817	T7980	T7979	dobj	exon,delete
R818	T8016	T8010	punct	", ",were
R819	T8017	T8010	conj	displayed,were
R820	T8018	T8019	det	no,abnormalities
R821	T8019	T8017	dobj	abnormalities,displayed
R822	T7981	T7980	nummod	3,exon
R823	T8020	T8019	amod	obvious,abnormalities
R824	T7982	T7979	prep	in,delete
R825	T8021	T8017	punct	", ",displayed
R826	T8022	T8017	cc	and,displayed
R827	T8023	T8017	conj	were,displayed
R828	T8024	T8023	acomp	fertile,were
R829	T8025	T8003	punct	.,born
R830	T7983	T7984	det	the,gene
R831	T8027	T8028	csubj	Crossing,failed
R832	T8029	T8030	amod	heterozygous,mice
R833	T8030	T8027	dobj	mice,Crossing
R834	T8031	T8032	aux	to,generate
R835	T8032	T8028	xcomp	generate,failed
R836	T8033	T8034	amod	viable,mice
R837	T8034	T8032	dobj	mice,generate
R838	T8035	T8034	amod	newborn,mice
R839	T8036	T8034	nmod	Chaf1a,mice
R840	T7984	T7982	pobj	gene,in
R841	T8037	T8036	punct	−,Chaf1a
R842	T8038	T8036	punct	/,Chaf1a
R843	T8039	T8036	punct	−,Chaf1a
R844	T8040	T8028	punct	.,failed
R845	T7985	T7984	compound	Chaf1a,gene
R846	T8042	T8043	advmod	Furthermore,detected
R847	T7986	T7984	punct	", ",gene
R848	T8044	T8043	punct	", ",detected
R849	T8045	T8046	det	no,embryos
R850	T8046	T8043	nsubjpass	embryos,detected
R851	T8047	T8046	amod	homozygous,embryos
R852	T7987	T7988	dep	which,encodes
R853	T8048	T8046	nmod	Chaf1a,embryos
R854	T8049	T8048	punct	−,Chaf1a
R855	T8050	T8048	punct	/,Chaf1a
R856	T7988	T7984	relcl	encodes,gene
R857	T8051	T8048	punct	−,Chaf1a
R858	T8052	T8043	auxpass	were,detected
R859	T8053	T8043	prep	at,detected
R860	T7989	T7988	dobj	p150CAF,encodes
R861	T8054	T8053	pobj	any,at
R862	T8055	T8054	prep	of,any
R863	T8056	T8057	det	the,stages
R864	T7990	T7989	punct	-,p150CAF
R865	T8057	T8055	pobj	stages,of
R866	T8058	T8057	amod	post-implantation,stages
R867	T8059	T8043	punct	.,detected
R868	T7991	T7989	nummod	1,p150CAF
R869	T8061	T8062	advmod	However,detect
R870	T7992	T7993	punct	(,1A
R871	T8063	T8062	punct	", ",detect
R872	T8064	T8062	nsubj	we,detect
R873	T8065	T8062	aux	could,detect
R874	T7993	T7972	parataxis	1A,used
R875	T8066	T8067	nmod	Chaf1a,embryos
R876	T8067	T8062	dobj	embryos,detect
R877	T8068	T8066	punct	−,Chaf1a
R878	T7994	T7993	compound	Figure,1A
R879	T8069	T8066	punct	/,Chaf1a
R880	T8070	T8066	punct	−,Chaf1a
R881	T8071	T8062	prep	at,detect
R882	T8072	T8073	amod	embryonic,day
R883	T8073	T8071	pobj	day,at
R884	T8074	T8073	nummod	4,day
R885	T8075	T8073	punct	(,day
R886	T7995	T7993	punct	),1A
R887	T8076	T8073	appos	E4,day
R888	T7996	T7972	punct	.,used
R889	T8077	T8062	punct	),detect
R890	T8078	T8062	advcl	using,detect
R891	T7998	T7999	nmod	Chaf1a,mice
R892	T8079	T8080	det	a,strategy
R893	T8080	T8078	dobj	strategy,using
R894	T8081	T8080	compound	PCR,strategy
R895	T8082	T8083	punct	(,1B
R896	T7999	T8003	nsubjpass	mice,born
R897	T8083	T8062	parataxis	1B,detect
R898	T8084	T8083	compound	Figure,1B
R899	T8085	T8083	punct	),1B
R900	T8000	T7998	punct	+,Chaf1a
R901	T8086	T8062	punct	.,detect
R902	T8088	T8089	nsubj	They,represent
R903	T8001	T7998	punct	/,Chaf1a
R904	T8090	T8091	advmod	only,10
R905	T8091	T8092	nummod	10,%
R906	T8002	T7998	punct	−,Chaf1a
R907	T8092	T8089	dobj	%,represent
R908	T8093	T8092	prep	of,%
R909	T8094	T8095	compound	E4,embryos
R910	T8004	T8003	auxpass	were,born
R911	T8095	T8093	pobj	embryos,of
R912	T8096	T8092	acl	obtained,%
R913	T8097	T8096	prep	from,obtained
R914	T8098	T8099	nmod	Chaf1a,intercrosses
R915	T8005	T8003	prep	at,born
R916	T8099	T8097	pobj	intercrosses,from
R917	T8100	T8098	punct	+,Chaf1a
R918	T8101	T8098	punct	/,Chaf1a
R919	T8006	T8007	det	a,frequency
R920	T8102	T8098	punct	−,Chaf1a
R921	T8103	T8099	compound	mice,intercrosses
R922	T8104	T8089	punct	", ",represent
R923	T8105	T8089	advcl	suggesting,represent
R924	T8007	T8005	pobj	frequency,at
R925	T8106	T8107	mark	that,degenerated
R926	T8107	T8105	ccomp	degenerated,suggesting
R927	T8008	T8007	amod	Mendelian,frequency
R928	T8108	T8109	amod	more,half
R929	T8109	T8107	nsubj	half,degenerated
R930	T8110	T8109	quantmod	than,half
R931	T8111	T8109	prep	of,half
R932	T8009	T8003	punct	", ",born
R933	T8112	T8113	det	the,embryos
R934	T8113	T8111	pobj	embryos,of
R935	T8114	T8115	amod	homozygous,mutant
R936	T8115	T8113	compound	mutant,embryos
R937	T8116	T8107	aux	had,degenerated
R938	T8010	T8003	conj	were,born
R939	T8223	T8224	advmod	Thus,allows
R940	T8117	T8107	prep	before,degenerated
R941	T8118	T8119	det	this,stage
R942	T8119	T8117	pobj	stage,before
R943	T8120	T8089	punct	.,represent
R944	T8225	T8224	punct	", ",allows
R945	T8122	T8123	advmod	Moreover,contained
R946	T8124	T8123	punct	", ",contained
R947	T8125	T8126	compound	mutant,embryos
R948	T8126	T8123	nsubj	embryos,contained
R949	T8226	T8224	nsubj	depletion,allows
R950	T8127	T8128	advmod	only,16
R951	T8128	T8131	nummod	16,cells
R952	T8129	T8128	quantmod	eight,16
R953	T8227	T8226	prep	of,depletion
R954	T8130	T8128	quantmod	to,16
R955	T8131	T8123	dobj	cells,contained
R956	T8132	T8123	prep	at,contained
R957	T8228	T8229	nmod	p150CAF,protein
R958	T8133	T8134	det	the,stage
R959	T8134	T8132	pobj	stage,at
R960	T8135	T8134	compound	E4,stage
R961	T8229	T8227	pobj	protein,of
R962	T8136	T8123	punct	", ",contained
R963	T8137	T8138	advmod	instead,of
R964	T8138	T8123	prep	of,contained
R965	T8230	T8228	punct	-,p150CAF
R966	T8139	T8140	nummod	32,cells
R967	T8140	T8138	pobj	cells,of
R968	T8141	T8140	acl	observed,cells
R969	T8231	T8228	nummod	1,p150CAF
R970	T8142	T8141	prep	for,observed
R971	T8143	T8144	amod	wild,type
R972	T8144	T8146	nmod	type,blastocysts
R973	T8232	T8226	prep	from,depletion
R974	T8145	T8144	punct	-,type
R975	T8146	T8142	pobj	blastocysts,for
R976	T8147	T8144	cc	or,type
R977	T8233	T8234	det	this,stage
R978	T8148	T8144	conj	heterozygous,type
R979	T8234	T8232	pobj	stage,from
R980	T8149	T8150	punct	(,1C
R981	T8150	T8123	parataxis	1C,contained
R982	T8151	T8150	compound	Figure,1C
R983	T8152	T8150	cc	and,1C
R984	T8235	T8236	det	a,maximum
R985	T8153	T8150	conj	1D,1C
R986	T8154	T8150	punct	),1C
R987	T8155	T8123	punct	.,contained
R988	T8157	T8158	amod	Further,analysis
R989	T8158	T8159	nsubj	analysis,allowed
R990	T8236	T8224	dobj	maximum,allows
R991	T8160	T8158	prep	by,analysis
R992	T8161	T8162	compound	light,microscopy
R993	T8237	T8236	prep	of,maximum
R994	T8162	T8160	pobj	microscopy,by
R995	T8238	T8239	nummod	two,divisions
R996	T8163	T8162	acl	using,microscopy
R997	T8164	T8163	dobj	immunofluorescence,using
R998	T8239	T8237	pobj	divisions,of
R999	T8165	T8166	punct	(,IF
R1000	T8166	T8164	parataxis	IF,immunofluorescence
R1001	T8167	T8166	punct	),IF
R1002	T8240	T8239	amod	additional,divisions
R1003	T8168	T8169	nsubj	us,compare
R1004	T8169	T8159	ccomp	compare,allowed
R1005	T8170	T8169	aux	to,compare
R1006	T8241	T8239	compound	cell,divisions
R1007	T8171	T8172	amod	wild,type
R1008	T8172	T8174	nmod	type,embryos
R1009	T8173	T8172	punct	-,type
R1010	T8242	T8224	prep	before,allows
R1011	T8174	T8169	dobj	embryos,compare
R1012	T8175	T8172	cc	and,type
R1013	T8176	T8177	npadvmod	p150CAF,depleted
R1014	T8177	T8172	conj	depleted,type
R1015	T8243	T8244	amod	developmental,arrest
R1016	T8244	T8242	pobj	arrest,before
R1017	T8178	T8176	punct	-,p150CAF
R1018	T8245	T8224	punct	.,allows
R1019	T8179	T8176	nummod	1,p150CAF
R1020	T8180	T8177	punct	-,depleted
R1021	T8247	T8248	advmod	Strikingly,found
R1022	T8181	T8159	punct	.,allowed
R1023	T8183	T8184	det	A,loss
R1024	T8184	T8185	nsubjpass	loss,detected
R1025	T8249	T8248	punct	", ",found
R1026	T8186	T8184	prep	of,loss
R1027	T8187	T8188	nmod	p150CAF,staining
R1028	T8188	T8186	pobj	staining,of
R1029	T8189	T8187	punct	-,p150CAF
R1030	T8250	T8248	nsubj	we,found
R1031	T8190	T8187	nummod	1,p150CAF
R1032	T8191	T8185	aux	could,detected
R1033	T8251	T8252	mark	that,appeared
R1034	T8192	T8185	neg	not,detected
R1035	T8193	T8185	auxpass	be,detected
R1036	T8194	T8185	prep	before,detected
R1037	T8195	T8196	det	the,stage
R1038	T8252	T8248	ccomp	appeared,found
R1039	T8196	T8194	pobj	stage,before
R1040	T8197	T8198	nummod	eight,cell
R1041	T8198	T8196	compound	cell,stage
R1042	T8199	T8198	punct	-,cell
R1043	T8200	T8201	punct	(,data
R1044	T8201	T8185	meta	data,detected
R1045	T8253	T8254	det	the,organization
R1046	T8202	T8201	amod	unpublished,data
R1047	T8203	T8201	punct	),data
R1048	T8204	T8185	advcl	suggesting,detected
R1049	T8254	T8252	nsubj	organization,appeared
R1050	T8205	T8206	mark	that,is
R1051	T8206	T8204	ccomp	is,suggesting
R1052	T8207	T8208	advmod	maternally,contributed
R1053	T8255	T8254	amod	nuclear,organization
R1054	T8208	T8209	amod	contributed,protein
R1055	T8209	T8206	nsubj	protein,is
R1056	T8210	T8206	acomp	present,is
R1057	T8256	T8254	prep	of,organization
R1058	T8211	T8206	prep	in,is
R1059	T8212	T8213	det	the,embryo
R1060	T8213	T8211	pobj	embryo,in
R1061	T8257	T8256	pobj	heterochromatin,of
R1062	T8214	T8206	prep	up,is
R1063	T8215	T8214	prep	to,up
R1064	T8216	T8217	det	the,stage
R1065	T8258	T8252	oprd	abnormal,appeared
R1066	T8217	T8215	pobj	stage,to
R1067	T8218	T8219	nummod	four,cell
R1068	T8219	T8217	compound	cell,stage
R1069	T8259	T8252	prep	in,appeared
R1070	T8220	T8219	punct	-,cell
R1071	T8221	T8185	punct	.,detected
R1072	T8260	T8261	det	the,nuclei
R1073	T8261	T8259	pobj	nuclei,in
R1074	T8262	T8261	prep	of,nuclei
R1075	T8328	T8326	acl	called,structures
R1076	T8329	T8328	oprd	chromocenters,called
R1077	T8263	T8264	nmod	Chaf1a,embryos
R1078	T8330	T8331	punct	[,20
R1079	T8331	T8328	parataxis	20,called
R1080	T8332	T8331	punct	],20
R1081	T8264	T8262	pobj	embryos,of
R1082	T8333	T8326	punct	", ",structures
R1083	T8334	T8335	dep	which,visualized
R1084	T8265	T8263	punct	−,Chaf1a
R1085	T8335	T8326	relcl	visualized,structures
R1086	T8266	T8263	punct	/,Chaf1a
R1087	T8336	T8335	auxpass	are,visualized
R1088	T8267	T8263	punct	−,Chaf1a
R1089	T8337	T8335	prep	as,visualized
R1090	T8338	T8339	npadvmod	DAPI,dense
R1091	T8339	T8341	amod	dense,foci
R1092	T8340	T8339	punct	-,dense
R1093	T8341	T8337	pobj	foci,as
R1094	T8342	T8343	punct	(,1C
R1095	T8268	T8248	punct	.,found
R1096	T8343	T8322	parataxis	1C,form
R1097	T8344	T8343	compound	Figure,1C
R1098	T8345	T8343	punct	),1C
R1099	T8270	T8271	amod	Pericentric,heterochromatin
R1100	T8346	T8306	punct	.,cluster
R1101	T8348	T8349	det	These,structures
R1102	T8349	T8350	nsubjpass	structures,detected
R1103	T8271	T8272	nsubjpass	heterochromatin,visualized
R1104	T8351	T8350	auxpass	were,detected
R1105	T8352	T8350	neg	not,detected
R1106	T8273	T8271	punct	", ",heterochromatin
R1107	T8353	T8350	prep	in,detected
R1108	T8354	T8355	nmod	Chaf1a,embryos
R1109	T8355	T8353	pobj	embryos,in
R1110	T8356	T8354	punct	−,Chaf1a
R1111	T8274	T8275	det	the,component
R1112	T8357	T8354	punct	/,Chaf1a
R1113	T8358	T8354	punct	−,Chaf1a
R1114	T8359	T8360	punct	(,1D
R1115	T8275	T8271	appos	component,heterochromatin
R1116	T8360	T8350	parataxis	1D,detected
R1117	T8361	T8360	compound	Figure,1D
R1118	T8362	T8360	punct	),1D
R1119	T8276	T8275	amod	major,component
R1120	T8363	T8350	punct	.,detected
R1121	T8365	T8366	mark	Since,are
R1122	T8277	T8275	prep	of,component
R1123	T8366	T8374	advcl	are,decided
R1124	T8367	T8368	compound	chromatin,architecture
R1125	T8368	T8366	nsubj	architecture,are
R1126	T8278	T8279	amod	constitutive,heterochromatin
R1127	T8369	T8368	cc	and,architecture
R1128	T8370	T8371	amod	higher,order
R1129	T8371	T8373	compound	order,structures
R1130	T8372	T8371	punct	-,order
R1131	T8279	T8277	pobj	heterochromatin,of
R1132	T8373	T8368	conj	structures,architecture
R1133	T8280	T8272	punct	", ",visualized
R1134	T8375	T8366	acomp	important,are
R1135	T8376	T8375	prep	for,important
R1136	T8377	T8378	compound	genome,function
R1137	T8281	T8272	aux	can,visualized
R1138	T8378	T8376	pobj	function,for
R1139	T8379	T8380	punct	[,21
R1140	T8282	T8272	advmod	easily,visualized
R1141	T8380	T8366	parataxis	21,are
R1142	T8381	T8380	punct	],21
R1143	T8382	T8374	punct	", ",decided
R1144	T8383	T8374	nsubj	we,decided
R1145	T8384	T8385	aux	to,characterize
R1146	T8385	T8374	xcomp	characterize,decided
R1147	T8283	T8272	auxpass	be,visualized
R1148	T8386	T8387	det	this,phenotype
R1149	T8387	T8385	dobj	phenotype,characterize
R1150	T8388	T8385	prep	in,characterize
R1151	T8284	T8272	prep	in,visualized
R1152	T8389	T8390	amod	more,detail
R1153	T8390	T8388	pobj	detail,in
R1154	T8391	T8374	punct	.,decided
R1155	T8285	T8286	compound	interphase,nuclei
R1156	T8393	T8394	prep	For,examined
R1157	T8286	T8284	pobj	nuclei,in
R1158	T8395	T8396	det	this,purpose
R1159	T8396	T8393	pobj	purpose,For
R1160	T8397	T8394	punct	", ",examined
R1161	T8287	T8272	prep	by,visualized
R1162	T8398	T8394	nsubj	we,examined
R1163	T8399	T8400	det	the,status
R1164	T8400	T8394	dobj	status,examined
R1165	T8288	T8289	det	the,DAPI
R1166	T8401	T8400	prep	of,status
R1167	T8402	T8403	compound	heterochromatin,organization
R1168	T8403	T8401	pobj	organization,of
R1169	T8289	T8287	pobj	DAPI,by
R1170	T8404	T8394	prep	between,examined
R1171	T8405	T8406	det	the,stages
R1172	T8406	T8404	pobj	stages,between
R1173	T8407	T8408	nummod	two,cell
R1174	T8290	T8289	compound	fluorochrome,DAPI
R1175	T8408	T8406	nmod	cell,stages
R1176	T8409	T8408	punct	-,cell
R1177	T8410	T8408	cc	and,cell
R1178	T8291	T8287	cc	and,by
R1179	T8411	T8408	conj	blastocyst,cell
R1180	T8412	T8394	punct	.,examined
R1181	T8292	T8287	conj	by,by
R1182	T8414	T8415	prep	In,is
R1183	T8293	T8292	pcomp	immunostaining,by
R1184	T8416	T8417	nummod	two,cell
R1185	T8417	T8419	nmod	cell,stage
R1186	T8294	T8293	prep	with,immunostaining
R1187	T8418	T8417	punct	-,cell
R1188	T8419	T8420	nmod	stage,embryos
R1189	T8420	T8414	pobj	embryos,In
R1190	T8295	T8294	pobj	HP1α,with
R1191	T8421	T8422	amod	wild,type
R1192	T8422	T8420	compound	type,embryos
R1193	T8423	T8422	punct	-,type
R1194	T8296	T8297	punct	[,16
R1195	T8424	T8415	punct	", ",is
R1196	T8425	T8426	compound	DAPI,staining
R1197	T8426	T8415	nsubj	staining,is
R1198	T8297	T8272	parataxis	16,visualized
R1199	T8427	T8415	acomp	diffuse,is
R1200	T8428	T8427	cc	and,diffuse
R1201	T8429	T8427	conj	fibrillar,diffuse
R1202	T8430	T8415	punct	", ",is
R1203	T8298	T8297	punct	",",16
R1204	T8431	T8415	prep	with,is
R1205	T8432	T8431	pobj	regions,with
R1206	T8299	T8297	appos	17,16
R1207	T8300	T8301	punct	–,19
R1208	T8301	T8299	prep	19,17
R1209	T8302	T8297	punct	],16
R1210	T8433	T8432	prep	of,regions
R1211	T8303	T8272	punct	.,visualized
R1212	T8434	T8435	amod	higher,density
R1213	T8435	T8433	pobj	density,of
R1214	T8436	T8432	prep	around,regions
R1215	T8305	T8306	prep	In,cluster
R1216	T8437	T8438	det	the,bodies
R1217	T8438	T8436	pobj	bodies,around
R1218	T8439	T8440	amod	nucleolar,precursor
R1219	T8307	T8308	amod	wild,type
R1220	T8440	T8438	compound	precursor,bodies
R1221	T8441	T8442	punct	(,1E
R1222	T8442	T8415	parataxis	1E,is
R1223	T8443	T8442	compound	Figure,1E
R1224	T8444	T8442	punct	),1E
R1225	T8308	T8310	compound	type,blastocysts
R1226	T8445	T8415	punct	.,is
R1227	T8447	T8448	compound	Heterochromatin,domains
R1228	T8309	T8308	punct	-,type
R1229	T8448	T8449	nsubjpass	domains,assembled
R1230	T8310	T8305	pobj	blastocysts,In
R1231	T8450	T8449	auxpass	are,assembled
R1232	T8451	T8449	advmod	progressively,assembled
R1233	T8452	T8449	prep	into,assembled
R1234	T8311	T8305	cc	and,In
R1235	T8453	T8454	npadvmod	DAPI,dense
R1236	T8454	T8456	amod	dense,foci
R1237	T8455	T8454	punct	-,dense
R1238	T8456	T8452	pobj	foci,into
R1239	T8312	T8305	conj	in,In
R1240	T8457	T8449	prep	between,assembled
R1241	T8458	T8459	det	the,stages
R1242	T8313	T8314	nmod	mouse,cells
R1243	T8459	T8457	pobj	stages,between
R1244	T8460	T8461	nummod	four,cell
R1245	T8461	T8459	nmod	cell,stages
R1246	T8462	T8461	punct	-,cell
R1247	T8463	T8461	cc	and,cell
R1248	T8464	T8465	nummod	32,cell
R1249	T8314	T8312	pobj	cells,in
R1250	T8465	T8461	conj	cell,cell
R1251	T8466	T8465	punct	-,cell
R1252	T8467	T8459	compound	blastocyst,stages
R1253	T8468	T8469	punct	(,1F
R1254	T8315	T8314	amod	somatic,cells
R1255	T8469	T8449	parataxis	1F,assembled
R1256	T8470	T8469	compound	Figure,1F
R1257	T8471	T8469	punct	),1F
R1258	T8472	T8449	punct	.,assembled
R1259	T8316	T8306	punct	", ",cluster
R1260	T8474	T8475	nsubj	Localization,confirmed
R1261	T8317	T8318	amod	pericentric,domains
R1262	T8476	T8474	prep	of,Localization
R1263	T8477	T8476	pobj	HP1α,of
R1264	T8318	T8306	nsubj	domains,cluster
R1265	T8478	T8479	mark	that,correspond
R1266	T8479	T8475	ccomp	correspond,confirmed
R1267	T8480	T8481	det	the,structures
R1268	T8319	T8318	compound	heterochromatin,domains
R1269	T8481	T8479	nsubj	structures,correspond
R1270	T8482	T8481	acl	visualized,structures
R1271	T8483	T8482	prep	by,visualized
R1272	T8320	T8306	advmod	together,cluster
R1273	T8484	T8485	compound	DAPI,staining
R1274	T8485	T8483	pobj	staining,by
R1275	T8486	T8479	prep	to,correspond
R1276	T8321	T8306	cc	and,cluster
R1277	T8487	T8488	amod	constitutive,heterochromatin
R1278	T8488	T8486	pobj	heterochromatin,to
R1279	T8489	T8490	punct	(,1E
R1280	T8322	T8306	conj	form,cluster
R1281	T8490	T8475	parataxis	1E,confirmed
R1282	T8491	T8490	compound	Figure,1E
R1283	T8492	T8490	punct	),1E
R1284	T8323	T8324	amod	higher,order
R1285	T8493	T8475	punct	.,confirmed
R1286	T8495	T8496	advmod	Thus,modified
R1287	T8324	T8326	compound	order,structures
R1288	T8497	T8496	punct	", ",modified
R1289	T8498	T8499	det	the,organization
R1290	T8325	T8324	punct	-,order
R1291	T8499	T8496	nsubjpass	organization,modified
R1292	T8500	T8499	amod	nuclear,organization
R1293	T8501	T8499	prep	of,organization
R1294	T8326	T8322	dobj	structures,form
R1295	T8502	T8501	pobj	heterochromatin,of
R1296	T8503	T8496	auxpass	is,modified
R1297	T8504	T8496	advmod	dramatically,modified
R1298	T8505	T8496	prep	during,modified
R1299	T8506	T8507	amod	pre-implantation,development
R1300	T8507	T8505	pobj	development,during
R1301	T8327	T8326	compound	chromatin,structures
R1302	T8508	T8496	punct	", ",modified
R1303	T8509	T8496	prep	between,modified
R1304	T8510	T8511	det	the,stages
R1305	T8538	T8534	conj	16,eight
R1306	T8511	T8509	pobj	stages,between
R1307	T8512	T8513	nummod	two,cell
R1308	T8513	T8511	nmod	cell,stages
R1309	T8514	T8513	punct	-,cell
R1310	T8515	T8513	cc	and,cell
R1311	T8539	T8535	punct	-,cell
R1312	T8516	T8513	conj	blastocyst,cell
R1313	T8517	T8496	punct	.,modified
R1314	T8540	T8520	punct	", ",were
R1315	T8519	T8520	prep	In,were
R1316	T8521	T8522	nmod	Chaf1a,embryos
R1317	T8541	T8542	npadvmod	DAPI,dense
R1318	T8522	T8519	pobj	embryos,In
R1319	T8523	T8521	punct	−,Chaf1a
R1320	T8542	T8544	amod	dense,foci
R1321	T8524	T8521	punct	/,Chaf1a
R1322	T8525	T8521	punct	−,Chaf1a
R1323	T8526	T8522	compound	E4,embryos
R1324	T8543	T8542	punct	-,dense
R1325	T8527	T8522	punct	", ",embryos
R1326	T8528	T8529	dep	which,arrested
R1327	T8529	T8522	relcl	arrested,embryos
R1328	T8544	T8520	nsubj	foci,were
R1329	T8530	T8529	auxpass	are,arrested
R1330	T8531	T8529	prep	between,arrested
R1331	T8532	T8533	det	the,stage
R1332	T8545	T8546	advmod	barely,detectable
R1333	T8533	T8531	pobj	stage,between
R1334	T8534	T8535	nummod	eight,cell
R1335	T8535	T8533	compound	cell,stage
R1336	T8546	T8520	acomp	detectable,were
R1337	T8536	T8534	punct	-,eight
R1338	T8537	T8534	cc	and,eight
R1339	T8547	T8548	punct	(,1D
R1340	T8548	T8520	parataxis	1D,were
R1341	T8549	T8548	compound	Figure,1D
R1342	T8550	T8548	punct	),1D
R1343	T8643	T8642	punct	-,type
R1344	T8551	T8520	punct	.,were
R1345	T8644	T8645	punct	(,compare
R1346	T8645	T8617	parataxis	compare,is
R1347	T8553	T8554	advmod	Instead,was
R1348	T8646	T8647	compound	Figure,1D
R1349	T8647	T8645	dobj	1D,compare
R1350	T8648	T8647	cc	and,1D
R1351	T8649	T8647	conj	1F,1D
R1352	T8650	T8645	punct	),compare
R1353	T8651	T8617	punct	.,is
R1354	T8653	T8654	det	These,data
R1355	T8654	T8655	nsubj	data,evidence
R1356	T8656	T8657	det	a,role
R1357	T8657	T8655	dobj	role,evidence
R1358	T8555	T8554	punct	", ",was
R1359	T8658	T8657	amod	key,role
R1360	T8659	T8657	prep	for,role
R1361	T8660	T8659	pobj	p150CAF,for
R1362	T8661	T8660	punct	-,p150CAF
R1363	T8556	T8557	compound	DAPI,staining
R1364	T8662	T8660	nummod	1,p150CAF
R1365	T8663	T8655	prep	during,evidence
R1366	T8664	T8665	amod	pre-implantation,development
R1367	T8665	T8663	pobj	development,during
R1368	T8557	T8554	nsubj	staining,was
R1369	T8666	T8655	punct	", ",evidence
R1370	T8667	T8655	cc	and,evidence
R1371	T8668	T8655	conj	reveal,evidence
R1372	T8558	T8554	acomp	diffuse,was
R1373	T8669	T8670	mark	that,required
R1374	T8670	T8668	ccomp	required,reveal
R1375	T8671	T8672	det	this,protein
R1376	T8559	T8554	prep	within,was
R1377	T8672	T8670	nsubjpass	protein,required
R1378	T8673	T8670	auxpass	is,required
R1379	T8674	T8670	prep	for,required
R1380	T8675	T8676	det	the,organization
R1381	T8560	T8561	det	the,nucleus
R1382	T8676	T8674	pobj	organization,for
R1383	T8677	T8676	amod	proper,organization
R1384	T8678	T8679	nummod	3,D
R1385	T8561	T8559	pobj	nucleus,within
R1386	T8679	T8676	compound	D,organization
R1387	T8680	T8679	punct	-,D
R1388	T8681	T8676	prep	of,organization
R1389	T8562	T8554	punct	", ",was
R1390	T8682	T8681	pobj	heterochromatin,of
R1391	T8563	T8554	prep	with,was
R1392	T8683	T8670	prep	within,required
R1393	T8564	T8563	pobj	regions,with
R1394	T8684	T8685	amod	embryonic,nuclei
R1395	T8685	T8683	pobj	nuclei,within
R1396	T8686	T8685	compound	cell,nuclei
R1397	T8565	T8564	prep	of,regions
R1398	T8687	T8655	punct	.,evidence
R1399	T8689	T8690	advmod	Next,wondered
R1400	T8566	T8567	amod	higher,density
R1401	T8691	T8690	punct	", ",wondered
R1402	T8692	T8690	nsubj	we,wondered
R1403	T8693	T8694	mark	whether,observed
R1404	T8694	T8690	ccomp	observed,wondered
R1405	T8695	T8696	det	a,requirement
R1406	T8696	T8694	nsubjpass	requirement,observed
R1407	T8567	T8565	pobj	density,of
R1408	T8697	T8696	amod	similar,requirement
R1409	T8698	T8696	prep	for,requirement
R1410	T8568	T8567	prep	around,density
R1411	T8699	T8698	pobj	CAF,for
R1412	T8700	T8699	punct	-,CAF
R1413	T8701	T8699	nummod	1,CAF
R1414	T8702	T8694	aux	could,observed
R1415	T8569	T8570	det	the,nucleoli
R1416	T8703	T8694	advmod	also,observed
R1417	T8704	T8694	auxpass	be,observed
R1418	T8705	T8694	prep	in,observed
R1419	T8570	T8568	pobj	nucleoli,around
R1420	T8706	T8707	compound	ES,cells
R1421	T8707	T8705	pobj	cells,in
R1422	T8708	T8707	punct	", ",cells
R1423	T8571	T8568	cc	and,around
R1424	T8709	T8710	dep	which,derived
R1425	T8710	T8707	relcl	derived,cells
R1426	T8711	T8710	auxpass	are,derived
R1427	T8712	T8710	prep	from,derived
R1428	T8572	T8568	conj	at,around
R1429	T8713	T8714	det	the,mass
R1430	T8714	T8712	pobj	mass,from
R1431	T8715	T8714	nmod	blastocyst,mass
R1432	T8716	T8717	amod	inner,cell
R1433	T8573	T8574	det	the,periphery
R1434	T8717	T8714	compound	cell,mass
R1435	T8718	T8690	punct	.,wondered
R1436	T8574	T8572	pobj	periphery,at
R1437	T8720	T8721	prep	Given,derived
R1438	T8722	T8723	det	the,arrest
R1439	T8575	T8574	prep	of,periphery
R1440	T8723	T8720	pobj	arrest,Given
R1441	T8724	T8723	amod	early,arrest
R1442	T8725	T8723	amod	developmental,arrest
R1443	T8576	T8577	det	the,nuclei
R1444	T8726	T8723	acl	observed,arrest
R1445	T8727	T8726	prep	in,observed
R1446	T8728	T8729	nmod	Chaf1a,embryos
R1447	T8577	T8575	pobj	nuclei,of
R1448	T8729	T8727	pobj	embryos,in
R1449	T8730	T8728	punct	−,Chaf1a
R1450	T8731	T8728	punct	/,Chaf1a
R1451	T8578	T8579	punct	(,1D
R1452	T8732	T8728	punct	−,Chaf1a
R1453	T8733	T8721	punct	", ",derived
R1454	T8734	T8735	amod	such,cells
R1455	T8735	T8721	nsubjpass	cells,derived
R1456	T8736	T8721	aux	could,derived
R1457	T8737	T8721	neg	not,derived
R1458	T8738	T8721	auxpass	be,derived
R1459	T8579	T8554	parataxis	1D,was
R1460	T8739	T8721	advmod	directly,derived
R1461	T8740	T8721	prep	from,derived
R1462	T8741	T8742	amod	null,embryos
R1463	T8580	T8579	compound	Figure,1D
R1464	T8742	T8740	pobj	embryos,from
R1465	T8743	T8721	punct	", ",derived
R1466	T8744	T8721	cc	and,derived
R1467	T8581	T8579	punct	),1D
R1468	T8745	T8746	nsubj	we,used
R1469	T8746	T8721	conj	used,derived
R1470	T8747	T8746	advmod	thus,used
R1471	T8748	T8749	det	an,strategy
R1472	T8582	T8554	punct	.,was
R1473	T8584	T8585	nsubj	Localization,showed
R1474	T8586	T8584	prep	of,Localization
R1475	T8749	T8746	dobj	strategy,used
R1476	T8750	T8749	compound	RNAi,strategy
R1477	T8751	T8752	punct	(,2A
R1478	T8587	T8586	pobj	HP1α,of
R1479	T8752	T8746	parataxis	2A,used
R1480	T8753	T8752	compound	Figure,2A
R1481	T8754	T8752	punct	),2A
R1482	T8755	T8721	punct	.,derived
R1483	T8588	T8589	det	a,pattern
R1484	T8757	T8758	nmod	p150CAF,knockdown
R1485	T8758	T8761	nsubjpass	knockdown,quantified
R1486	T8589	T8585	dobj	pattern,showed
R1487	T8759	T8757	punct	-,p150CAF
R1488	T8760	T8757	nummod	1,p150CAF
R1489	T8590	T8589	amod	diffuse,pattern
R1490	T8762	T8758	prep	in,knockdown
R1491	T8763	T8764	compound	ES,cells
R1492	T8764	T8762	pobj	cells,in
R1493	T8591	T8589	amod	similar,pattern
R1494	T8765	T8761	auxpass	was,quantified
R1495	T8766	T8761	prep	by,quantified
R1496	T8592	T8591	prep	to,similar
R1497	T8767	T8768	compound	Western,blot
R1498	T8768	T8769	compound	blot,analysis
R1499	T8769	T8766	pobj	analysis,by
R1500	T8593	T8594	det	the,DAPI
R1501	T8594	T8592	pobj	DAPI,to
R1502	T8770	T8769	cc	and,analysis
R1503	T8595	T8589	punct	", ",pattern
R1504	T8771	T8769	conj	IF,analysis
R1505	T8772	T8761	punct	.,quantified
R1506	T8774	T8775	punct	(,S1
R1507	T8776	T8775	compound	Figure,S1
R1508	T8777	T8775	punct	),S1
R1509	T8596	T8589	prep	with,pattern
R1510	T8778	T8775	punct	.,S1
R1511	T8780	T8781	nmod	p150CAF,cells
R1512	T8597	T8598	det	some,enrichment
R1513	T8781	T8788	nsubj	cells,displayed
R1514	T8782	T8780	punct	-,p150CAF
R1515	T8598	T8596	pobj	enrichment,with
R1516	T8783	T8780	nummod	1,p150CAF
R1517	T8784	T8785	npadvmod	RNAi,depleted
R1518	T8785	T8781	amod	depleted,cells
R1519	T8599	T8598	prep	at,enrichment
R1520	T8786	T8785	punct	-,depleted
R1521	T8787	T8781	compound	ES,cells
R1522	T8600	T8601	det	the,periphery
R1523	T8789	T8790	det	a,phenotype
R1524	T8790	T8788	dobj	phenotype,displayed
R1525	T8791	T8792	advmod	very,similar
R1526	T8792	T8790	amod	similar,phenotype
R1527	T8601	T8599	pobj	periphery,at
R1528	T8793	T8792	prep	to,similar
R1529	T8794	T8795	det	the,cells
R1530	T8795	T8793	pobj	cells,to
R1531	T8602	T8601	amod	nuclear,periphery
R1532	T8796	T8795	prep	of,cells
R1533	T8797	T8798	nmod	Chaf1a,mice
R1534	T8798	T8796	pobj	mice,of
R1535	T8799	T8797	punct	−,Chaf1a
R1536	T8603	T8599	punct	", ",at
R1537	T8800	T8797	punct	/,Chaf1a
R1538	T8801	T8797	punct	−,Chaf1a
R1539	T8802	T8798	compound	E4,mice
R1540	T8604	T8599	cc	and,at
R1541	T8803	T8788	punct	.,displayed
R1542	T8805	T8806	npadvmod	DAPI,dense
R1543	T8605	T8599	conj	around,at
R1544	T8806	T8808	amod	dense,foci
R1545	T8807	T8806	punct	-,dense
R1546	T8808	T8809	nsubjpass	foci,lost
R1547	T8606	T8607	det	the,nucleoli
R1548	T8810	T8809	auxpass	were,lost
R1549	T8811	T8809	punct	", ",lost
R1550	T8812	T8809	cc	and,lost
R1551	T8607	T8605	pobj	nucleoli,around
R1552	T8813	T8814	nsubj	we,observed
R1553	T8814	T8809	conj	observed,lost
R1554	T8815	T8816	amod	diffuse,staining
R1555	T8608	T8609	punct	(,1D
R1556	T8816	T8814	dobj	staining,observed
R1557	T8817	T8816	nmod	HP1α,staining
R1558	T8818	T8817	cc	and,HP1α
R1559	T8819	T8817	conj	DAPI,HP1α
R1560	T8820	T8814	prep	around,observed
R1561	T8821	T8822	det	the,nucleoli
R1562	T8822	T8820	pobj	nucleoli,around
R1563	T8609	T8585	parataxis	1D,showed
R1564	T8823	T8820	cc	and,around
R1565	T8824	T8820	conj	at,around
R1566	T8610	T8609	compound	Figure,1D
R1567	T8825	T8826	det	the,periphery
R1568	T8826	T8824	pobj	periphery,at
R1569	T8827	T8826	prep	of,periphery
R1570	T8828	T8829	det	the,nucleus
R1571	T8829	T8827	pobj	nucleus,of
R1572	T8611	T8609	punct	),1D
R1573	T8830	T8831	punct	(,2B
R1574	T8831	T8814	parataxis	2B,observed
R1575	T8832	T8831	compound	Figure,2B
R1576	T8612	T8585	punct	.,showed
R1577	T8833	T8831	punct	),2B
R1578	T8834	T8814	punct	.,observed
R1579	T8614	T8615	det	This,organization
R1580	T8836	T8837	det	This,result
R1581	T8837	T8838	nsubj	result,indicates
R1582	T8839	T8840	mark	that,is
R1583	T8615	T8617	nsubj	organization,is
R1584	T8840	T8838	ccomp	is,indicates
R1585	T8841	T8840	nsubj	p150CAF,is
R1586	T8842	T8841	punct	-,p150CAF
R1587	T8616	T8615	amod	abnormal,organization
R1588	T8843	T8841	nummod	1,p150CAF
R1589	T8844	T8840	acomp	essential,is
R1590	T8845	T8844	prep	for,essential
R1591	T8618	T8615	prep	of,organization
R1592	T8846	T8847	amod	nuclear,organization
R1593	T8847	T8845	pobj	organization,for
R1594	T8848	T8847	prep	of,organization
R1595	T8849	T8848	pobj	heterochromatin,of
R1596	T8619	T8618	pobj	heterochromatin,of
R1597	T8850	T8849	prep	in,heterochromatin
R1598	T8851	T8852	compound	ES,cells
R1599	T8852	T8850	pobj	cells,in
R1600	T8620	T8615	prep	in,organization
R1601	T8853	T8847	prep	in,organization
R1602	T8621	T8622	nmod	Chaf1a,embryos
R1603	T8622	T8620	pobj	embryos,in
R1604	T8854	T8855	det	a,way
R1605	T8623	T8621	punct	−,Chaf1a
R1606	T8855	T8853	pobj	way,in
R1607	T8856	T8855	amod	similar,way
R1608	T8857	T8855	prep	to,way
R1609	T8624	T8621	punct	/,Chaf1a
R1610	T8858	T8859	amod	early,embryos
R1611	T8859	T8857	pobj	embryos,to
R1612	T8860	T8859	amod	pre-implantation,embryos
R1613	T8861	T8838	punct	.,indicates
R1614	T8625	T8621	punct	−,Chaf1a
R1615	T8863	T8864	advmod	Surprisingly,observe
R1616	T8626	T8617	acomp	reminiscent,is
R1617	T8865	T8864	punct	", ",observe
R1618	T8866	T8864	nsubj	we,observe
R1619	T8867	T8864	aux	did,observe
R1620	T8868	T8864	neg	not,observe
R1621	T8627	T8626	prep	of,reminiscent
R1622	T8869	T8870	det	this,alteration
R1623	T8628	T8629	det	the,organization
R1624	T8870	T8864	dobj	alteration,observe
R1625	T8871	T8870	amod	severe,alteration
R1626	T8872	T8870	prep	in,alteration
R1627	T8873	T8874	compound	heterochromatin,organization
R1628	T8629	T8627	pobj	organization,of
R1629	T8874	T8872	pobj	organization,in
R1630	T8875	T8864	prep	in,observe
R1631	T8876	T8877	amod	primary,fibroblasts
R1632	T8630	T8629	compound	heterochromatin,organization
R1633	T8877	T8875	pobj	fibroblasts,in
R1634	T8878	T8877	nmod	mouse,fibroblasts
R1635	T8879	T8877	amod	embryonic,fibroblasts
R1636	T8880	T8877	punct	(,fibroblasts
R1637	T8631	T8629	acl	found,organization
R1638	T8881	T8877	appos	MEFs,fibroblasts
R1639	T8882	T8864	punct	),observe
R1640	T8883	T8864	prep	following,observe
R1641	T8632	T8631	prep	in,found
R1642	T8884	T8885	nmod	p150CAF,depletion
R1643	T8885	T8883	pobj	depletion,following
R1644	T8886	T8884	punct	-,p150CAF
R1645	T8633	T8634	quantmod	two,four
R1646	T8887	T8884	nummod	1,p150CAF
R1647	T8888	T8885	prep	by,depletion
R1648	T8889	T8888	pobj	RNAi,by
R1649	T8634	T8637	nummod	four,cell
R1650	T8890	T8864	punct	.,observe
R1651	T8892	T8893	nmod	p150CAF,depletion
R1652	T8635	T8634	punct	-,four
R1653	T8893	T8896	nsubj	depletion,resulted
R1654	T8894	T8892	punct	-,p150CAF
R1655	T8895	T8892	nummod	1,p150CAF
R1656	T8636	T8634	quantmod	to,four
R1657	T8897	T8893	prep	in,depletion
R1658	T8898	T8897	pobj	MEFs,in
R1659	T8637	T8639	nmod	cell,stage
R1660	T8899	T8896	prep	in,resulted
R1661	T8900	T8901	det	a,inhibition
R1662	T8901	T8899	pobj	inhibition,in
R1663	T8902	T8901	amod	strong,inhibition
R1664	T8903	T8901	prep	of,inhibition
R1665	T8904	T8905	compound	cell,proliferation
R1666	T8905	T8903	pobj	proliferation,of
R1667	T8638	T8637	punct	-,cell
R1668	T8906	T8907	punct	(,data
R1669	T8907	T8896	meta	data,resulted
R1670	T8908	T8907	amod	unpublished,data
R1671	T8909	T8907	punct	),data
R1672	T8639	T8640	nmod	stage,embryos
R1673	T8910	T8896	punct	", ",resulted
R1674	T8911	T8896	cc	but,resulted
R1675	T8912	T8913	aux	did,alter
R1676	T8913	T8896	conj	alter,resulted
R1677	T8640	T8632	pobj	embryos,in
R1678	T8914	T8913	neg	not,alter
R1679	T8915	T8916	det	the,clustering
R1680	T8916	T8913	dobj	clustering,alter
R1681	T8641	T8642	amod	wild,type
R1682	T8917	T8916	prep	of,clustering
R1683	T8918	T8919	compound	heterochromatin,domains
R1684	T8919	T8917	pobj	domains,of
R1685	T8642	T8640	compound	type,embryos
R1686	T8920	T8921	punct	(,2C
R1687	T8921	T8913	parataxis	2C,alter
R1688	T8922	T8921	compound	Figures,2C
R1689	T8959	T8957	pobj	embryo,in
R1690	T8923	T8921	cc	and,2C
R1691	T8924	T8921	conj	S2,2C
R1692	T8925	T8921	punct	),2C
R1693	T8926	T8896	punct	.,resulted
R1694	T8928	T8929	advmod	Similarly,affect
R1695	T8960	T8959	amod	early,embryo
R1696	T8930	T8929	punct	", ",affect
R1697	T8931	T8932	nmod	p150CAF,depletion
R1698	T8961	T8959	cc	and,embryo
R1699	T8932	T8929	nsubj	depletion,affect
R1700	T8933	T8931	punct	-,p150CAF
R1701	T8962	T8963	compound	ES,cells
R1702	T8934	T8931	nummod	1,p150CAF
R1703	T8935	T8932	prep	in,depletion
R1704	T8936	T8937	compound	3T3,cells
R1705	T8937	T8935	pobj	cells,in
R1706	T8963	T8959	conj	cells,embryo
R1707	T8938	T8929	aux	did,affect
R1708	T8939	T8929	neg	not,affect
R1709	T8940	T8941	compound	heterochromatin,organization
R1710	T8964	T8949	punct	.,reveal
R1711	T8941	T8929	dobj	organization,affect
R1712	T8942	T8943	punct	[,16
R1713	T8943	T8929	parataxis	16,affect
R1714	T8944	T8943	punct	],16
R1715	T8945	T8929	punct	.,affect
R1716	T8966	T8967	nsubj	We,wondered
R1717	T8947	T8948	det	These,observations
R1718	T8948	T8949	nsubj	observations,reveal
R1719	T8950	T8951	det	a,function
R1720	T8968	T8967	advmod	also,wondered
R1721	T8951	T8949	dobj	function,reveal
R1722	T8952	T8951	amod	specific,function
R1723	T8953	T8951	prep	for,function
R1724	T8954	T8953	pobj	CAF,for
R1725	T8955	T8954	punct	-,CAF
R1726	T8956	T8954	nummod	1,CAF
R1727	T8969	T8970	mark	whether,affects
R1728	T8957	T8951	prep	in,function
R1729	T8958	T8959	det	the,embryo
R1730	T8970	T8967	advcl	affects,wondered
R1731	T8971	T8970	nsubj	loss,affects
R1732	T8972	T8971	prep	of,loss
R1733	T8973	T8972	pobj	p150CAF,of
R1734	T9065	T9064	compound	Figure,2D
R1735	T8974	T8973	punct	-,p150CAF
R1736	T9066	T9064	punct	),2D
R1737	T9067	T9038	punct	.,detect
R1738	T8975	T8973	nummod	1,p150CAF
R1739	T9069	T9070	det	These,data
R1740	T9070	T9071	nsubj	data,show
R1741	T8976	T8971	prep	in,loss
R1742	T9072	T9073	mark	that,altered
R1743	T9073	T9071	ccomp	altered,show
R1744	T8977	T8978	compound	ES,cells
R1745	T9074	T9075	amod	nuclear,architecture
R1746	T9075	T9073	nsubjpass	architecture,altered
R1747	T9076	T9073	auxpass	is,altered
R1748	T8978	T8976	pobj	cells,in
R1749	T9077	T9073	neg	not,altered
R1750	T9078	T9073	advmod	globally,altered
R1751	T8979	T8970	advmod	specifically,affects
R1752	T9079	T9073	prep	following,altered
R1753	T9080	T9079	pobj	CAF,following
R1754	T9081	T9080	punct	-,CAF
R1755	T8980	T8981	det	the,subcompartment
R1756	T9082	T9080	nummod	1,CAF
R1757	T9083	T9080	appos	loss,CAF
R1758	T9084	T9083	punct	-,loss
R1759	T8981	T8970	dobj	subcompartment,affects
R1760	T9085	T9083	prep	of,loss
R1761	T9086	T9085	punct	-,of
R1762	T9087	T9085	pobj	function,of
R1763	T8982	T8981	nmod	heterochromatin,subcompartment
R1764	T9088	T9071	punct	.,show
R1765	T9090	T9091	prep	In,revealed
R1766	T9092	T9090	pobj	addition,In
R1767	T9093	T9091	punct	", ",revealed
R1768	T9094	T9095	compound	Western,blot
R1769	T8983	T8981	amod	nuclear,subcompartment
R1770	T9095	T9096	compound	blot,analysis
R1771	T9096	T9091	nsubj	analysis,revealed
R1772	T9097	T9098	mark	that,result
R1773	T8984	T8970	punct	", ",affects
R1774	T9098	T9091	ccomp	result,revealed
R1775	T9099	T9100	nmod	CAF,depletion
R1776	T9100	T9098	nsubj	depletion,result
R1777	T8985	T8970	cc	or,affects
R1778	T9101	T9099	punct	-,CAF
R1779	T9102	T9099	nummod	1,CAF
R1780	T9103	T9098	aux	did,result
R1781	T8986	T8987	mark	whether,result
R1782	T9104	T9098	neg	not,result
R1783	T9105	T9098	prep	in,result
R1784	T9106	T9107	amod	altered,levels
R1785	T8987	T8970	conj	result,affects
R1786	T9107	T9105	pobj	levels,in
R1787	T9108	T9107	prep	of,levels
R1788	T9109	T9110	nmod	chromatin,proteins
R1789	T8988	T8987	nsubj	it,result
R1790	T9110	T9108	pobj	proteins,of
R1791	T9111	T9110	amod	architectural,proteins
R1792	T9112	T9113	amod	such,as
R1793	T9113	T9110	prep	as,proteins
R1794	T8989	T8987	aux	might,result
R1795	T9114	T9115	compound	histone,H3
R1796	T9115	T9113	pobj	H3,as
R1797	T9116	T9115	cc	and,H3
R1798	T8990	T8987	prep	in,result
R1799	T9117	T9115	conj	HP1α,H3
R1800	T9118	T9119	punct	(,S1
R1801	T8991	T8992	det	a,loss
R1802	T9119	T9091	parataxis	S1,revealed
R1803	T8992	T8990	pobj	loss,in
R1804	T9120	T9119	compound	Figure,S1
R1805	T9121	T9119	punct	),S1
R1806	T9122	T9091	punct	.,revealed
R1807	T8993	T8994	advmod	more,global
R1808	T9124	T9125	advmod	Thus,required
R1809	T8994	T8992	amod	global,loss
R1810	T9126	T9125	punct	", ",required
R1811	T9127	T9125	nsubjpass	CAF,required
R1812	T8995	T8992	prep	of,loss
R1813	T9128	T9127	punct	-,CAF
R1814	T9129	T9127	nummod	1,CAF
R1815	T9130	T9125	auxpass	is,required
R1816	T8996	T8997	amod	nuclear,organization
R1817	T9131	T9125	advmod	specifically,required
R1818	T9132	T9125	prep	in,required
R1819	T9133	T9134	amod	early,embryos
R1820	T9134	T9132	pobj	embryos,in
R1821	T9135	T9132	cc	and,in
R1822	T9136	T9132	conj	in,in
R1823	T8997	T8995	pobj	organization,of
R1824	T9137	T9138	compound	ES,cells
R1825	T9138	T9136	pobj	cells,in
R1826	T9139	T9125	prep	for,required
R1827	T8998	T8997	cc	and,organization
R1828	T9140	T9141	det	the,organization
R1829	T9141	T9139	pobj	organization,for
R1830	T9142	T9141	amod	proper,organization
R1831	T8999	T8997	conj	architecture,organization
R1832	T9143	T9141	prep	of,organization
R1833	T9144	T9145	det	the,compartment
R1834	T9145	T9143	pobj	compartment,of
R1835	T9000	T8967	punct	.,wondered
R1836	T9146	T9145	nmod	heterochromatin,compartment
R1837	T9147	T9145	amod	subnuclear,compartment
R1838	T9148	T9125	punct	.,required
R1839	T9002	T9003	nsubj	We,used
R1840	T9150	T9151	amod	Previous,studies
R1841	T9151	T9152	nsubj	studies,showed
R1842	T9004	T9005	det	a,antibody
R1843	T9153	T9151	acl	performed,studies
R1844	T9154	T9153	prep	in,performed
R1845	T9155	T9156	amod	mammalian,lines
R1846	T9156	T9154	pobj	lines,in
R1847	T9157	T9156	compound	cell,lines
R1848	T9005	T9003	dobj	antibody,used
R1849	T9158	T9159	mark	that,required
R1850	T9159	T9152	ccomp	required,showed
R1851	T9160	T9161	nmod	CAF,activity
R1852	T9161	T9159	nsubjpass	activity,required
R1853	T9006	T9005	amod	specific,antibody
R1854	T9162	T9160	punct	-,CAF
R1855	T9163	T9160	nummod	1,CAF
R1856	T9164	T9159	auxpass	is,required
R1857	T9007	T9005	prep	against,antibody
R1858	T9165	T9159	prep	for,required
R1859	T9166	T9167	compound	S,phase
R1860	T9008	T9009	det	the,protein
R1861	T9167	T9168	compound	phase,progression
R1862	T9168	T9165	pobj	progression,for
R1863	T9169	T9170	punct	[,24
R1864	T9009	T9007	pobj	protein,against
R1865	T9170	T9152	parataxis	24,showed
R1866	T9010	T9011	amod	promyelocytic,leukemia
R1867	T9011	T9009	nmod	leukemia,protein
R1868	T9171	T9170	nummod	16,24
R1869	T9172	T9170	punct	",",24
R1870	T9173	T9170	nummod	23,24
R1871	T9174	T9170	punct	",",24
R1872	T9175	T9170	punct	],24
R1873	T9012	T9011	punct	(,leukemia
R1874	T9176	T9152	punct	.,showed
R1875	T9178	T9179	nsubj	We,show
R1876	T9013	T9011	appos	PML,leukemia
R1877	T9180	T9179	advmod	here,show
R1878	T9181	T9182	mark	that,appear
R1879	T9014	T9009	punct	),protein
R1880	T9015	T9016	aux	to,study
R1881	T9182	T9179	ccomp	appear,show
R1882	T9183	T9184	nummod	3,d
R1883	T9184	T9185	npadvmod	d,after
R1884	T9016	T9003	advcl	study,used
R1885	T9185	T9182	prep	after,appear
R1886	T9186	T9185	pobj	transfection,after
R1887	T9017	T9018	det	the,fate
R1888	T9187	T9186	prep	of,transfection
R1889	T9188	T9189	det	the,vector
R1890	T9189	T9187	pobj	vector,of
R1891	T9018	T9016	dobj	fate,study
R1892	T9190	T9189	compound	RNAi,vector
R1893	T9191	T9182	punct	", ",appear
R1894	T9192	T9193	npadvmod	p150CAF,depleted
R1895	T9019	T9018	prep	of,fate
R1896	T9193	T9197	amod	depleted,cells
R1897	T9194	T9192	punct	-,p150CAF
R1898	T9195	T9192	nummod	1,p150CAF
R1899	T9020	T9021	det	the,bodies
R1900	T9196	T9193	punct	-,depleted
R1901	T9197	T9182	nsubj	cells,appear
R1902	T9198	T9197	punct	(,cells
R1903	T9021	T9019	pobj	bodies,of
R1904	T9199	T9197	acl	identified,cells
R1905	T9200	T9199	agent	by,identified
R1906	T9201	T9202	det	a,absence
R1907	T9022	T9021	nmod	PML,bodies
R1908	T9202	T9200	pobj	absence,by
R1909	T9203	T9202	amod	complete,absence
R1910	T9204	T9202	prep	of,absence
R1911	T9023	T9021	amod	nuclear,bodies
R1912	T9205	T9206	nmod	CAF,signal
R1913	T9206	T9204	pobj	signal,of
R1914	T9207	T9205	punct	-,CAF
R1915	T9024	T9021	punct	", ",bodies
R1916	T9208	T9205	nummod	1,CAF
R1917	T9209	T9206	compound	IF,signal
R1918	T9210	T9197	punct	),cells
R1919	T9025	T9026	dep	which,are
R1920	T9211	T9212	advmod	still,active
R1921	T9212	T9182	oprd	active,appear
R1922	T9213	T9212	prep	for,active
R1923	T9214	T9213	pobj	replication,for
R1924	T9215	T9182	punct	", ",appear
R1925	T9216	T9217	mark	as,revealed
R1926	T9026	T9021	relcl	are,bodies
R1927	T9217	T9182	advcl	revealed,appear
R1928	T9218	T9217	agent	by,revealed
R1929	T9219	T9218	pobj	incorporation,by
R1930	T9027	T9028	advmod	well,characterized
R1931	T9220	T9219	prep	of,incorporation
R1932	T9221	T9222	det	the,analog
R1933	T9028	T9030	amod	characterized,structures
R1934	T9222	T9220	pobj	analog,of
R1935	T9223	T9222	compound	thymidine,analog
R1936	T9224	T9222	appos	bromodeoxyuridine,analog
R1937	T9225	T9224	punct	(,bromodeoxyuridine
R1938	T9029	T9028	punct	-,characterized
R1939	T9226	T9224	appos	BrdU,bromodeoxyuridine
R1940	T9227	T9219	punct	),incorporation
R1941	T9228	T9229	punct	(,3A
R1942	T9030	T9026	attr	structures,are
R1943	T9229	T9219	parataxis	3A,incorporation
R1944	T9230	T9229	compound	Figure,3A
R1945	T9031	T9030	amod	subnuclear,structures
R1946	T9231	T9229	punct	),3A
R1947	T9232	T9219	punct	", ",incorporation
R1948	T9233	T9234	det	the,pattern
R1949	T9032	T9033	punct	[,22
R1950	T9234	T9219	conj	pattern,incorporation
R1951	T9235	T9234	nmod	PCNA,pattern
R1952	T9236	T9235	punct	(,PCNA
R1953	T9033	T9003	parataxis	22,used
R1954	T9237	T9238	amod	proliferating,antigen
R1955	T9238	T9235	appos	antigen,PCNA
R1956	T9239	T9238	nmod	cell,antigen
R1957	T9034	T9033	punct	],22
R1958	T9240	T9238	amod	nuclear,antigen
R1959	T9241	T9234	punct	),pattern
R1960	T9242	T9243	punct	(,3B
R1961	T9035	T9003	punct	.,used
R1962	T9243	T9234	parataxis	3B,pattern
R1963	T9244	T9243	compound	Figure,3B
R1964	T9245	T9243	punct	),3B
R1965	T9246	T9234	punct	", ",pattern
R1966	T9037	T9038	nsubj	We,detect
R1967	T9247	T9234	cc	and,pattern
R1968	T9248	T9249	compound	flow,analysis
R1969	T9249	T9234	conj	analysis,pattern
R1970	T9250	T9249	compound	cytometry,analysis
R1971	T9039	T9038	aux	could,detect
R1972	T9251	T9252	punct	(,3C
R1973	T9252	T9249	parataxis	3C,analysis
R1974	T9253	T9252	compound	Figure,3C
R1975	T9254	T9252	punct	),3C
R1976	T9255	T9179	punct	.,show
R1977	T9257	T9258	advmod	Hence,persists
R1978	T9259	T9258	punct	", ",persists
R1979	T9040	T9038	neg	not,detect
R1980	T9260	T9258	prep	in,persists
R1981	T9261	T9262	det	these,cells
R1982	T9262	T9260	pobj	cells,in
R1983	T9263	T9258	punct	", ",persists
R1984	T9041	T9042	det	any,difference
R1985	T9264	T9265	compound	DNA,replication
R1986	T9265	T9258	nsubj	replication,persists
R1987	T9266	T9258	prep	despite,persists
R1988	T9042	T9038	dobj	difference,detect
R1989	T9267	T9268	det	the,changes
R1990	T9268	T9266	pobj	changes,despite
R1991	T9269	T9268	amod	drastic,changes
R1992	T9043	T9042	amod	significant,difference
R1993	T9270	T9268	prep	in,changes
R1994	T9271	T9272	compound	heterochromatin,organization
R1995	T9272	T9270	pobj	organization,in
R1996	T9044	T9042	prep	in,difference
R1997	T9273	T9258	punct	.,persists
R1998	T9275	T9276	advmod	However,cease
R1999	T9045	T9046	det	the,distribution
R2000	T9046	T9044	pobj	distribution,in
R2001	T9047	T9046	cc	and,distribution
R2002	T9277	T9276	punct	", ",cease
R2003	T9048	T9046	conj	aspect,distribution
R2004	T9278	T9279	nummod	24,h
R2005	T9279	T9280	npadvmod	h,later
R2006	T9280	T9276	advmod	later,cease
R2007	T9049	T9046	prep	of,distribution
R2008	T9281	T9276	punct	", ",cease
R2009	T9282	T9283	compound	ES,cells
R2010	T9283	T9276	nsubj	cells,cease
R2011	T9284	T9285	aux	to,proliferate
R2012	T9050	T9051	nmod	PML,bodies
R2013	T9285	T9276	xcomp	proliferate,cease
R2014	T9286	T9276	cc	and,cease
R2015	T9051	T9049	pobj	bodies,of
R2016	T9287	T9276	conj	die,cease
R2017	T9288	T9276	punct	", ",cease
R2018	T9289	T9276	advcl	revealing,cease
R2019	T9290	T9291	mark	that,required
R2020	T9052	T9051	amod	nuclear,bodies
R2021	T9053	T9051	prep	between,bodies
R2022	T9291	T9289	ccomp	required,revealing
R2023	T9292	T9291	nsubjpass	p150CAF,required
R2024	T9054	T9055	nmod	control,cells
R2025	T9293	T9292	punct	-,p150CAF
R2026	T9294	T9292	nummod	1,p150CAF
R2027	T9295	T9291	auxpass	is,required
R2028	T9296	T9291	prep	for,required
R2029	T9297	T9298	det	an,process
R2030	T9298	T9296	pobj	process,for
R2031	T9299	T9298	amod	essential,process
R2032	T9055	T9053	pobj	cells,between
R2033	T9300	T9298	amod	cellular,process
R2034	T9301	T9291	punct	", ",required
R2035	T9302	T9291	prep	as,required
R2036	T9056	T9054	cc	and,control
R2037	T9303	T9302	prep	in,as
R2038	T9304	T9303	pobj	embryos,in
R2039	T9305	T9276	punct	.,cease
R2040	T9057	T9058	npadvmod	p150CAF,depleted
R2041	T9307	T9308	advmod	Altogether,demonstrate
R2042	T9058	T9054	conj	depleted,control
R2043	T9309	T9308	punct	", ",demonstrate
R2044	T9310	T9311	poss	our,data
R2045	T9059	T9057	punct	-,p150CAF
R2046	T9311	T9308	nsubj	data,demonstrate
R2047	T9312	T9313	mark	that,alters
R2048	T9313	T9308	ccomp	alters,demonstrate
R2049	T9060	T9057	nummod	1,p150CAF
R2050	T9314	T9313	nsubj	loss,alters
R2051	T9315	T9314	prep	of,loss
R2052	T9316	T9317	nmod	p150CAF,function
R2053	T9061	T9058	punct	-,depleted
R2054	T9317	T9315	pobj	function,of
R2055	T9318	T9316	punct	-,p150CAF
R2056	T9319	T9316	nummod	1,p150CAF
R2057	T9062	T9055	compound	ES,cells
R2058	T9320	T9314	prep	in,loss
R2059	T9321	T9322	compound	ES,cells
R2060	T9322	T9320	pobj	cells,in
R2061	T9063	T9064	punct	(,2D
R2062	T9323	T9322	cc	and,cells
R2063	T9324	T9325	amod	early,embryos
R2064	T9325	T9322	conj	embryos,cells
R2065	T9064	T9038	parataxis	2D,detect
R2066	T9326	T9313	advmod	first,alters
R2067	T9327	T9328	det	the,organization
R2068	T9328	T9313	dobj	organization,alters
R2069	T9382	T9379	amod	chromosomal,regions
R2070	T9329	T9328	prep	of,organization
R2071	T9330	T9329	pobj	heterochromatin,of
R2072	T9331	T9328	prep	in,organization
R2073	T9332	T9333	det	the,nucleus
R2074	T9333	T9331	pobj	nucleus,in
R2075	T9334	T9313	cc	and,alters
R2076	T9383	T9379	punct	", ",regions
R2077	T9335	T9313	punct	", ",alters
R2078	T9336	T9337	prep	in,cycle
R2079	T9337	T9313	conj	cycle,alters
R2080	T9338	T9339	det	a,step
R2081	T9339	T9336	pobj	step,in
R2082	T9340	T9339	amod	subsequent,step
R2083	T9341	T9337	punct	", ",cycle
R2084	T9342	T9337	compound	cell,cycle
R2085	T9384	T9385	dep	which,composed
R2086	T9343	T9337	cc	and,cycle
R2087	T9344	T9337	conj	viability,cycle
R2088	T9345	T9308	punct	.,demonstrate
R2089	T9385	T9379	relcl	composed,regions
R2090	T9347	T9348	prep	In,performed
R2091	T9349	T9347	pobj	order,In
R2092	T9350	T9351	aux	to,characterize
R2093	T9386	T9385	prep	in,composed
R2094	T9351	T9349	acl	characterize,order
R2095	T9352	T9351	advmod	better,characterize
R2096	T9353	T9354	det	the,defects
R2097	T9387	T9386	pobj	mouse,in
R2098	T9354	T9351	dobj	defects,characterize
R2099	T9355	T9354	prep	in,defects
R2100	T9388	T9385	auxpass	are,composed
R2101	T9389	T9385	advmod	mainly,composed
R2102	T9356	T9357	compound	heterochromatin,organization
R2103	T9390	T9385	prep	of,composed
R2104	T9357	T9355	pobj	organization,in
R2105	T9358	T9348	punct	", ",performed
R2106	T9359	T9348	nsubj	we,performed
R2107	T9391	T9392	amod	large,blocks
R2108	T9360	T9361	nummod	two,color
R2109	T9361	T9363	nmod	color,experiments
R2110	T9362	T9361	punct	-,color
R2111	T9392	T9390	pobj	blocks,of
R2112	T9363	T9348	dobj	experiments,performed
R2113	T9364	T9363	nmod	DNA,experiments
R2114	T9365	T9366	nmod	fluorescence,hybridization
R2115	T9393	T9392	prep	of,blocks
R2116	T9366	T9363	nmod	hybridization,experiments
R2117	T9367	T9368	advmod	in,situ
R2118	T9368	T9366	amod	situ,hybridization
R2119	T9369	T9366	punct	(,hybridization
R2120	T9394	T9395	amod	major,repeats
R2121	T9370	T9366	appos	FISH,hybridization
R2122	T9371	T9363	punct	),experiments
R2123	T9372	T9373	aux	to,reveal
R2124	T9395	T9393	pobj	repeats,of
R2125	T9373	T9348	advcl	reveal,performed
R2126	T9374	T9375	det	the,distribution
R2127	T9396	T9394	cc	and,major
R2128	T9375	T9373	dobj	distribution,reveal
R2129	T9376	T9375	amod	spatial,distribution
R2130	T9377	T9375	prep	of,distribution
R2131	T9378	T9379	amod	pericentric,regions
R2132	T9397	T9394	conj	minor,major
R2133	T9379	T9377	pobj	regions,of
R2134	T9380	T9378	cc	and,pericentric
R2135	T9381	T9378	conj	centric,pericentric
R2136	T9398	T9395	compound	satellite,repeats
R2137	T9399	T9385	punct	", ",composed
R2138	T9400	T9385	advmod	respectively,composed
R2139	T9401	T9402	punct	[,20
R2140	T9488	T9486	punct	),4B
R2141	T9489	T9459	punct	.,observed
R2142	T9402	T9348	parataxis	20,performed
R2143	T9491	T9492	amod	Individual,domains
R2144	T9403	T9402	punct	],20
R2145	T9492	T9494	nsubjpass	domains,found
R2146	T9493	T9492	amod	pericentric,domains
R2147	T9404	T9348	punct	.,performed
R2148	T9495	T9492	prep	from,domains
R2149	T9496	T9497	amod	single,chromosomes
R2150	T9497	T9495	pobj	chromosomes,from
R2151	T9498	T9494	auxpass	are,found
R2152	T9406	T9407	amod	Pericentric,domains
R2153	T9499	T9494	advmod	now,found
R2154	T9500	T9501	preconj	either,isolated
R2155	T9501	T9494	oprd	isolated,found
R2156	T9502	T9501	cc	or,isolated
R2157	T9407	T9408	nsubj	domains,form
R2158	T9503	T9501	conj	aggregated,isolated
R2159	T9504	T9501	prep	in,isolated
R2160	T9409	T9407	prep	from,domains
R2161	T9505	T9506	det	a,pattern
R2162	T9506	T9504	pobj	pattern,in
R2163	T9507	T9508	advmod	less,dense
R2164	T9508	T9506	amod	dense,pattern
R2165	T9410	T9411	amod	different,chromosomes
R2166	T9509	T9508	prep	than,dense
R2167	T9510	T9511	det	the,clusters
R2168	T9511	T9509	pobj	clusters,than
R2169	T9411	T9409	pobj	chromosomes,from
R2170	T9512	T9511	amod	regular,clusters
R2171	T9513	T9511	acl	observed,clusters
R2172	T9514	T9513	prep	in,observed
R2173	T9412	T9408	dobj	clusters,form
R2174	T9515	T9516	compound	control,cells
R2175	T9516	T9514	pobj	cells,in
R2176	T9517	T9518	punct	(,4B
R2177	T9413	T9412	punct	", ",clusters
R2178	T9518	T9494	parataxis	4B,found
R2179	T9519	T9518	compound	Figure,4B
R2180	T9520	T9518	punct	),4B
R2181	T9521	T9494	punct	.,found
R2182	T9523	T9524	amod	Such,aggregates
R2183	T9414	T9415	dep	which,revealed
R2184	T9524	T9525	nsubjpass	aggregates,revealed
R2185	T9526	T9524	punct	", ",aggregates
R2186	T9415	T9412	relcl	revealed,clusters
R2187	T9527	T9528	dep	which,found
R2188	T9528	T9524	relcl	found,aggregates
R2189	T9529	T9528	auxpass	are,found
R2190	T9416	T9415	auxpass	are,revealed
R2191	T9530	T9528	advmod	often,found
R2192	T9531	T9528	prep	at,found
R2193	T9532	T9533	det	the,periphery
R2194	T9417	T9415	prep	by,revealed
R2195	T9533	T9531	pobj	periphery,at
R2196	T9534	T9533	amod	nuclear,periphery
R2197	T9535	T9525	punct	", ",revealed
R2198	T9418	T9417	pobj	FISH,by
R2199	T9536	T9525	auxpass	are,revealed
R2200	T9537	T9525	advmod	also,revealed
R2201	T9538	T9525	prep	by,revealed
R2202	T9419	T9415	prep	as,revealed
R2203	T9539	T9540	compound	DAPI,staining
R2204	T9540	T9538	pobj	staining,by
R2205	T9541	T9525	prep	as,revealed
R2206	T9420	T9421	amod	large,spots
R2207	T9542	T9543	det	a,signature
R2208	T9543	T9541	pobj	signature,as
R2209	T9544	T9543	amod	typical,signature
R2210	T9421	T9419	pobj	spots,as
R2211	T9545	T9543	prep	of,signature
R2212	T9546	T9545	pobj	p150CAF,of
R2213	T9547	T9546	punct	-,p150CAF
R2214	T9422	T9423	dep	that,coincide
R2215	T9548	T9546	nummod	1,p150CAF
R2216	T9423	T9421	relcl	coincide,spots
R2217	T9549	T9546	appos	loss,p150CAF
R2218	T9550	T9549	punct	-,loss
R2219	T9424	T9423	prep	with,coincide
R2220	T9551	T9549	prep	of,loss
R2221	T9552	T9551	punct	-,of
R2222	T9425	T9426	npadvmod	DAPI,dense
R2223	T9553	T9551	pobj	function,of
R2224	T9554	T9525	punct	.,revealed
R2225	T9556	T9557	nsubj	Quantification,revealed
R2226	T9426	T9428	amod	dense,foci
R2227	T9558	T9556	prep	of,Quantification
R2228	T9559	T9558	pobj	fluorescence,of
R2229	T9427	T9426	punct	-,dense
R2230	T9560	T9556	prep	along,Quantification
R2231	T9561	T9562	det	a,line
R2232	T9428	T9424	pobj	foci,with
R2233	T9562	T9560	pobj	line,along
R2234	T9563	T9564	advmod	randomly,drawn
R2235	T9564	T9562	acl	drawn,line
R2236	T9565	T9564	prep	across,drawn
R2237	T9566	T9567	det	the,nucleus
R2238	T9567	T9565	pobj	nucleus,across
R2239	T9568	T9569	amod	lower,intensity
R2240	T9429	T9423	prep	in,coincide
R2241	T9569	T9557	dobj	intensity,revealed
R2242	T9570	T9569	compound	fluorescence,intensity
R2243	T9571	T9569	cc	and,intensity
R2244	T9430	T9431	det	the,nucleus
R2245	T9572	T9573	det	a,distribution
R2246	T9573	T9569	conj	distribution,intensity
R2247	T9574	T9573	amod	broader,distribution
R2248	T9431	T9429	pobj	nucleus,in
R2249	T9575	T9573	prep	of,distribution
R2250	T9576	T9575	pobj	DAPI,of
R2251	T9577	T9576	cc	and,DAPI
R2252	T9578	T9579	amod	major,signals
R2253	T9432	T9431	compound	interphase,nucleus
R2254	T9579	T9576	conj	signals,DAPI
R2255	T9580	T9579	compound	satellite,signals
R2256	T9433	T9434	punct	(,4A
R2257	T9581	T9579	compound	hybridization,signals
R2258	T9582	T9557	prep	in,revealed
R2259	T9583	T9584	npadvmod	p150CAF,depleted
R2260	T9584	T9588	amod	depleted,cells
R2261	T9434	T9423	parataxis	4A,coincide
R2262	T9585	T9583	punct	-,p150CAF
R2263	T9586	T9583	nummod	1,p150CAF
R2264	T9587	T9584	punct	-,depleted
R2265	T9435	T9434	compound	Figure,4A
R2266	T9588	T9582	pobj	cells,in
R2267	T9589	T9590	punct	(,4C
R2268	T9590	T9557	parataxis	4C,revealed
R2269	T9436	T9434	punct	),4A
R2270	T9591	T9590	compound	Figure,4C
R2271	T9592	T9590	cc	and,4C
R2272	T9593	T9590	conj	4D,4C
R2273	T9437	T9408	punct	.,form
R2274	T9439	T9440	prep	At,form
R2275	T9594	T9590	punct	),4C
R2276	T9441	T9442	det	the,periphery
R2277	T9595	T9557	punct	", ",revealed
R2278	T9596	T9557	advcl	indicating,revealed
R2279	T9597	T9596	dobj	decondensation,indicating
R2280	T9598	T9597	prep	of,decondensation
R2281	T9442	T9439	pobj	periphery,At
R2282	T9599	T9600	amod	pericentric,domains
R2283	T9600	T9598	pobj	domains,of
R2284	T9601	T9600	compound	heterochromatin,domains
R2285	T9602	T9557	punct	.,revealed
R2286	T9604	T9605	prep	In,showed
R2287	T9443	T9442	prep	of,periphery
R2288	T9606	T9604	pobj	contrast,In
R2289	T9607	T9605	punct	", ",showed
R2290	T9608	T9609	amod	centric,domains
R2291	T9444	T9445	det	each,domain
R2292	T9609	T9605	nsubj	domains,showed
R2293	T9610	T9611	det	an,intensity
R2294	T9445	T9443	pobj	domain,of
R2295	T9611	T9605	dobj	intensity,showed
R2296	T9612	T9611	cc	and,intensity
R2297	T9613	T9611	conj	shape,intensity
R2298	T9446	T9445	amod	pericentric,domain
R2299	T9614	T9611	amod	similar,intensity
R2300	T9615	T9614	prep	to,similar
R2301	T9616	T9617	compound	control,cells
R2302	T9447	T9440	punct	", ",form
R2303	T9617	T9615	pobj	cells,to
R2304	T9618	T9605	punct	", ",showed
R2305	T9619	T9605	advcl	suggesting,showed
R2306	T9448	T9449	amod	centric,regions
R2307	T9620	T9621	mark	that,remain
R2308	T9621	T9619	ccomp	remain,suggesting
R2309	T9622	T9621	nsubj	they,remain
R2310	T9449	T9440	nsubj	regions,form
R2311	T9623	T9621	acomp	unaffected,remain
R2312	T9624	T9623	prep	by,unaffected
R2313	T9625	T9626	nmod	p150CAF,depletion
R2314	T9450	T9451	amod	individual,entities
R2315	T9626	T9624	pobj	depletion,by
R2316	T9627	T9625	punct	-,p150CAF
R2317	T9628	T9625	nummod	1,p150CAF
R2318	T9451	T9440	dobj	entities,form
R2319	T9629	T9630	punct	(,4C
R2320	T9630	T9605	parataxis	4C,showed
R2321	T9631	T9630	compound	Figure,4C
R2322	T9452	T9453	punct	(,4A
R2323	T9632	T9630	cc	and,4C
R2324	T9453	T9440	parataxis	4A,form
R2325	T9633	T9630	conj	4D,4C
R2326	T9634	T9630	punct	),4C
R2327	T9635	T9605	punct	.,showed
R2328	T9454	T9453	compound	Figure,4A
R2329	T9637	T9638	prep	In,show
R2330	T9455	T9453	punct	),4A
R2331	T9639	T9637	pobj	conclusion,In
R2332	T9640	T9638	punct	", ",show
R2333	T9641	T9642	det	these,results
R2334	T9642	T9638	nsubj	results,show
R2335	T9456	T9440	punct	.,form
R2336	T9643	T9644	mark	that,required
R2337	T9644	T9638	ccomp	required,show
R2338	T9645	T9644	nsubjpass	p150CAF,required
R2339	T9646	T9645	punct	-,p150CAF
R2340	T9647	T9645	nummod	1,p150CAF
R2341	T9458	T9459	prep	In,observed
R2342	T9648	T9644	auxpass	is,required
R2343	T9649	T9644	prep	for,required
R2344	T9650	T9651	det	the,condensation
R2345	T9651	T9649	pobj	condensation,for
R2346	T9652	T9651	amod	proper,condensation
R2347	T9460	T9461	nmod	p150CAF,cells
R2348	T9653	T9651	cc	and,condensation
R2349	T9654	T9651	conj	clustering,condensation
R2350	T9655	T9651	prep	of,condensation
R2351	T9656	T9657	amod	pericentric,domains
R2352	T9461	T9458	pobj	cells,In
R2353	T9657	T9655	pobj	domains,of
R2354	T9658	T9657	compound	heterochromatin,domains
R2355	T9462	T9460	punct	-,p150CAF
R2356	T9659	T9638	punct	.,show
R2357	T9661	T9662	prep	Given,wondered
R2358	T9463	T9460	nummod	1,p150CAF
R2359	T9663	T9664	det	the,role
R2360	T9664	T9661	pobj	role,Given
R2361	T9464	T9465	npadvmod	RNAi,depleted
R2362	T9665	T9664	amod	known,role
R2363	T9666	T9664	prep	of,role
R2364	T9667	T9666	pobj	CAF,of
R2365	T9465	T9461	amod	depleted,cells
R2366	T9668	T9667	punct	-,CAF
R2367	T9669	T9667	nummod	1,CAF
R2368	T9670	T9664	prep	in,role
R2369	T9466	T9465	punct	-,depleted
R2370	T9671	T9672	det	the,deposition
R2371	T9672	T9670	pobj	deposition,in
R2372	T9673	T9672	prep	of,deposition
R2373	T9674	T9675	compound	histone,H3.1
R2374	T9467	T9459	punct	", ",observed
R2375	T9675	T9673	pobj	H3.1,of
R2376	T9676	T9675	cc	and,H3.1
R2377	T9677	T9675	conj	H4,H3.1
R2378	T9468	T9459	nsubj	we,observed
R2379	T9678	T9675	acl	associated,H3.1
R2380	T9679	T9678	prep	with,associated
R2381	T9680	T9681	compound	DNA,synthesis
R2382	T9469	T9470	det	a,disruption
R2383	T9681	T9679	pobj	synthesis,with
R2384	T9682	T9683	punct	[,12
R2385	T9683	T9664	parataxis	12,role
R2386	T9470	T9459	dobj	disruption,observed
R2387	T9684	T9683	nummod	11,12
R2388	T9685	T9683	punct	",",12
R2389	T9686	T9683	punct	],12
R2390	T9687	T9662	punct	", ",wondered
R2391	T9688	T9662	nsubj	we,wondered
R2392	T9689	T9690	mark	whether,reflect
R2393	T9690	T9662	ccomp	reflect,wondered
R2394	T9691	T9692	det	this,defect
R2395	T9471	T9470	prep	of,disruption
R2396	T9692	T9690	nsubj	defect,reflect
R2397	T9693	T9692	prep	in,defect
R2398	T9694	T9695	amod	higher,order
R2399	T9472	T9473	amod	pericentric,clusters
R2400	T9695	T9697	compound	order,organization
R2401	T9696	T9695	punct	-,order
R2402	T9697	T9693	pobj	organization,in
R2403	T9698	T9697	compound	chromatin,organization
R2404	T9473	T9471	pobj	clusters,of
R2405	T9699	T9690	aux	could,reflect
R2406	T9474	T9473	compound	heterochromatin,clusters
R2407	T9475	T9476	dep	that,coincides
R2408	T9476	T9470	relcl	coincides,disruption
R2409	T9477	T9476	prep	with,coincides
R2410	T9700	T9701	det	an,organization
R2411	T9701	T9690	dobj	organization,reflect
R2412	T9478	T9479	det	the,disappearance
R2413	T9702	T9701	amod	aberrant,organization
R2414	T9703	T9701	amod	nucleosomal,organization
R2415	T9704	T9662	punct	.,wondered
R2416	T9479	T9477	pobj	disappearance,with
R2417	T9706	T9707	advcl	Using,observe
R2418	T9480	T9479	prep	of,disappearance
R2419	T9708	T9706	dobj	DNase,Using
R2420	T9709	T9708	nummod	I,DNase
R2421	T9481	T9482	npadvmod	DAPI,dense
R2422	T9710	T9708	cc	and,DNase
R2423	T9711	T9712	amod	micrococcal,nuclease
R2424	T9712	T9713	nmod	nuclease,assays
R2425	T9482	T9484	amod	dense,foci
R2426	T9713	T9708	conj	assays,DNase
R2427	T9714	T9712	punct	(,nuclease
R2428	T9715	T9712	appos	MNase,nuclease
R2429	T9483	T9482	punct	-,dense
R2430	T9716	T9713	punct	),assays
R2431	T9717	T9707	punct	", ",observe
R2432	T9718	T9707	nsubj	we,observe
R2433	T9484	T9480	pobj	foci,of
R2434	T9719	T9707	aux	could,observe
R2435	T9485	T9486	punct	(,4B
R2436	T9720	T9707	neg	not,observe
R2437	T9486	T9459	parataxis	4B,observed
R2438	T9721	T9722	det	any,difference
R2439	T9722	T9707	dobj	difference,observe
R2440	T9723	T9722	amod	significant,difference
R2441	T9724	T9722	prep	between,difference
R2442	T9725	T9726	nmod	control,cells
R2443	T9487	T9486	compound	Figure,4B
R2444	T9726	T9724	pobj	cells,between
R2445	T9727	T9725	cc	and,control
R2446	T9728	T9729	npadvmod	p150CAF,depleted
R2447	T9729	T9725	conj	depleted,control
R2448	T9806	T9807	det	all,conditions
R2449	T9730	T9728	punct	-,p150CAF
R2450	T9731	T9728	nummod	1,p150CAF
R2451	T9732	T9729	punct	-,depleted
R2452	T9733	T9726	compound	ES,cells
R2453	T9734	T9707	prep	at,observe
R2454	T9735	T9736	det	the,level
R2455	T9807	T9804	pobj	conditions,In
R2456	T9736	T9734	pobj	level,at
R2457	T9737	T9736	prep	of,level
R2458	T9738	T9739	compound	bulk,chromatin
R2459	T9739	T9737	pobj	chromatin,of
R2460	T9808	T9805	punct	", ",was
R2461	T9740	T9739	compound	genome,chromatin
R2462	T9741	T9734	cc	or,at
R2463	T9809	T9810	det	the,amount
R2464	T9742	T9734	conj	at,at
R2465	T9810	T9805	nsubj	amount,was
R2466	T9743	T9744	amod	pericentric,repeats
R2467	T9744	T9742	pobj	repeats,at
R2468	T9811	T9810	prep	of,amount
R2469	T9745	T9746	punct	(,Figure
R2470	T9746	T9707	parataxis	Figure,observe
R2471	T9747	T9746	nummod	5,Figure
R2472	T9748	T9746	punct	),Figure
R2473	T9812	T9813	compound	histone,H3
R2474	T9749	T9707	punct	.,observe
R2475	T9751	T9752	prep	In,compared
R2476	T9813	T9811	pobj	H3,of
R2477	T9753	T9754	det	a,series
R2478	T9814	T9810	acl	remaining,amount
R2479	T9754	T9751	pobj	series,In
R2480	T9755	T9754	amod	second,series
R2481	T9756	T9754	prep	of,series
R2482	T9815	T9810	acl	associated,amount
R2483	T9757	T9756	pobj	experiments,of
R2484	T9758	T9752	punct	", ",compared
R2485	T9759	T9752	nsubj	we,compared
R2486	T9816	T9815	prep	with,associated
R2487	T9760	T9761	det	the,association
R2488	T9761	T9752	dobj	association,compared
R2489	T9762	T9761	prep	of,association
R2490	T9763	T9764	compound	histone,H3
R2491	T9764	T9762	pobj	H3,of
R2492	T9817	T9816	pobj	chromatin,with
R2493	T9765	T9752	prep	with,compared
R2494	T9766	T9765	pobj	chromatin,with
R2495	T9767	T9752	prep	in,compared
R2496	T9768	T9767	pobj	nuclei,in
R2497	T9769	T9768	acl	isolated,nuclei
R2498	T9770	T9769	prep	from,isolated
R2499	T9771	T9770	pobj	cells,from
R2500	T9772	T9771	acl	transfected,cells
R2501	T9818	T9805	acomp	indistinguishable,was
R2502	T9773	T9772	prep	with,transfected
R2503	T9774	T9775	nmod	control,vectors
R2504	T9775	T9773	pobj	vectors,with
R2505	T9776	T9774	cc	and,control
R2506	T9819	T9805	prep	in,was
R2507	T9777	T9774	conj	p150CAF,control
R2508	T9778	T9777	punct	-,p150CAF
R2509	T9779	T9777	nummod	1,p150CAF
R2510	T9820	T9821	nmod	control,cells
R2511	T9780	T9775	compound	RNAi,vectors
R2512	T9781	T9775	compound	plasmid,vectors
R2513	T9782	T9752	punct	.,compared
R2514	T9821	T9819	pobj	cells,in
R2515	T9784	T9785	nsubjpass	Nuclei,incubated
R2516	T9786	T9785	auxpass	were,incubated
R2517	T9822	T9820	cc	and,control
R2518	T9787	T9785	prep	in,incubated
R2519	T9788	T9787	pobj	buffers,in
R2520	T9823	T9824	npadvmod	p150CAF,depleted
R2521	T9789	T9788	prep	with,buffers
R2522	T9790	T9791	amod	different,concentrations
R2523	T9791	T9789	pobj	concentrations,with
R2524	T9824	T9820	conj	depleted,control
R2525	T9792	T9791	compound	salt,concentrations
R2526	T9793	T9791	acl	ranging,concentrations
R2527	T9794	T9793	prep	from,ranging
R2528	T9795	T9796	nummod	100,mM
R2529	T9825	T9823	punct	-,p150CAF
R2530	T9796	T9797	compound	mM,NaCl
R2531	T9797	T9794	pobj	NaCl,from
R2532	T9798	T9794	prep	to,from
R2533	T9826	T9823	nummod	1,p150CAF
R2534	T9799	T9800	nummod	1,M
R2535	T9800	T9801	compound	M,NaCl
R2536	T9801	T9798	pobj	NaCl,to
R2537	T9802	T9785	punct	.,incubated
R2538	T9827	T9824	punct	-,depleted
R2539	T9804	T9805	prep	In,was
R2540	T9828	T9821	compound	ES,cells
R2541	T9829	T9830	punct	(,data
R2542	T9830	T9805	meta	data,was
R2543	T9831	T9830	amod	unpublished,data
R2544	T9912	T9909	parataxis	25,methylation
R2545	T9913	T9912	punct	],25
R2546	T9914	T9906	prep	at,tested
R2547	T9915	T9916	amod	major,repeats
R2548	T9832	T9830	punct	),data
R2549	T9916	T9914	pobj	repeats,at
R2550	T9917	T9916	compound	satellite,repeats
R2551	T9918	T9906	cc	and,tested
R2552	T9919	T9906	conj	found,tested
R2553	T9833	T9805	punct	.,was
R2554	T9920	T9921	det	no,difference
R2555	T9921	T9919	dobj	difference,found
R2556	T9922	T9921	amod	significant,difference
R2557	T9923	T9921	prep	between,difference
R2558	T9835	T9836	advmod	Therefore,are
R2559	T9924	T9925	nmod	control,cells
R2560	T9925	T9923	pobj	cells,between
R2561	T9926	T9924	cc	and,control
R2562	T9927	T9928	npadvmod	p150CAF,depleted
R2563	T9928	T9924	conj	depleted,control
R2564	T9837	T9836	punct	", ",are
R2565	T9929	T9927	punct	-,p150CAF
R2566	T9930	T9927	nummod	1,p150CAF
R2567	T9931	T9928	punct	-,depleted
R2568	T9932	T9933	punct	(,Figure
R2569	T9838	T9839	det	the,loss
R2570	T9933	T9919	parataxis	Figure,found
R2571	T9934	T9933	nummod	6,Figure
R2572	T9935	T9933	punct	),Figure
R2573	T9839	T9836	nsubj	loss,are
R2574	T9936	T9906	punct	.,tested
R2575	T9938	T9939	mark	As,is
R2576	T9840	T9839	prep	of,loss
R2577	T9939	T9952	advcl	is,reduced
R2578	T9940	T9941	det	the,line
R2579	T9941	T9939	nsubj	line,is
R2580	T9841	T9840	pobj	clustering,of
R2581	T9942	T9941	compound	LTM7,line
R2582	T9943	T9941	compound	ES,line
R2583	T9944	T9941	compound	cell,line
R2584	T9842	T9839	cc	and,loss
R2585	T9945	T9946	dep	that,used
R2586	T9946	T9941	relcl	used,line
R2587	T9947	T9946	nsubj	we,used
R2588	T9948	T9946	prep	in,used
R2589	T9843	T9839	conj	decondensation,loss
R2590	T9949	T9950	poss	our,experiments
R2591	T9950	T9948	pobj	experiments,in
R2592	T9951	T9950	compound	RNAi,experiments
R2593	T9844	T9843	prep	of,decondensation
R2594	T9953	T9954	det	a,line
R2595	T9954	T9939	attr	line,is
R2596	T9845	T9846	amod	pericentric,heterochromatin
R2597	T9955	T9954	amod	female,line
R2598	T9956	T9957	punct	(,XX
R2599	T9957	T9955	parataxis	XX,female
R2600	T9958	T9957	punct	),XX
R2601	T9959	T9954	compound	cell,line
R2602	T9960	T9952	punct	", ",reduced
R2603	T9961	T9962	compound	DNA,methylation
R2604	T9962	T9952	nsubjpass	methylation,reduced
R2605	T9846	T9844	pobj	heterochromatin,of
R2606	T9963	T9952	auxpass	is,reduced
R2607	T9964	T9952	advmod	globally,reduced
R2608	T9965	T9952	prep	in,reduced
R2609	T9847	T9836	acomp	unlikely,are
R2610	T9966	T9967	det	these,cells
R2611	T9967	T9965	pobj	cells,in
R2612	T9968	T9969	punct	[,26
R2613	T9969	T9952	parataxis	26,reduced
R2614	T9848	T9849	aux	to,be
R2615	T9970	T9969	punct	],26
R2616	T9971	T9952	punct	.,reduced
R2617	T9849	T9847	xcomp	be,unlikely
R2618	T9973	T9974	aux	To,rule
R2619	T9974	T9975	advcl	rule,tested
R2620	T9976	T9974	prt	out,rule
R2621	T9977	T9978	det	the,possibility
R2622	T9978	T9974	dobj	possibility,rule
R2623	T9979	T9980	mark	that,contribute
R2624	T9850	T9851	det	the,consequence
R2625	T9851	T9849	attr	consequence,be
R2626	T9980	T9978	acl	contribute,possibility
R2627	T9981	T9980	nsubj	hypomethylation,contribute
R2628	T9982	T9981	prep	of,hypomethylation
R2629	T9983	T9984	amod	pericentric,repeats
R2630	T9852	T9851	prep	of,consequence
R2631	T9984	T9982	pobj	repeats,of
R2632	T9985	T9980	aux	might,contribute
R2633	T9986	T9980	prep	to,contribute
R2634	T9987	T9988	det	the,destabilization
R2635	T9853	T9854	amod	severe,defects
R2636	T9988	T9986	pobj	destabilization,to
R2637	T9989	T9988	prep	of,destabilization
R2638	T9990	T9991	compound	heterochromatin,domains
R2639	T9854	T9852	pobj	defects,of
R2640	T9991	T9989	pobj	domains,of
R2641	T9992	T9980	prep	in,contribute
R2642	T9993	T9994	npadvmod	p150CAF,depleted
R2643	T9994	T9998	amod	depleted,cells
R2644	T9995	T9993	punct	-,p150CAF
R2645	T9855	T9854	prep	in,defects
R2646	T9996	T9993	nummod	1,p150CAF
R2647	T9997	T9994	punct	-,depleted
R2648	T9998	T9992	pobj	cells,in
R2649	T9999	T9998	compound	ES,cells
R2650	T10000	T9975	punct	", ",tested
R2651	T10001	T9975	nsubj	we,tested
R2652	T10002	T10003	poss	our,vector
R2653	T10003	T9975	dobj	vector,tested
R2654	T9856	T9857	amod	nucleosomal,organization
R2655	T10004	T10003	compound	RNAi,vector
R2656	T10005	T10003	compound	plasmid,vector
R2657	T10006	T9975	prep	in,tested
R2658	T10007	T10008	det	a,line
R2659	T9857	T9855	pobj	organization,in
R2660	T10008	T10006	pobj	line,in
R2661	T10009	T10008	compound	XY,line
R2662	T10010	T10008	compound	ES,line
R2663	T9858	T9836	punct	.,are
R2664	T10011	T10008	compound	cell,line
R2665	T10012	T9975	punct	", ",tested
R2666	T10013	T9975	cc	and,tested
R2667	T9860	T9861	poss	Our,findings
R2668	T10014	T9975	conj	confirmed,tested
R2669	T10015	T10016	mark	that,leads
R2670	T10016	T10014	ccomp	leads,confirmed
R2671	T9861	T9862	nsubj	findings,showed
R2672	T9863	T9864	mark	that,altered
R2673	T10017	T10016	nsubj	loss,leads
R2674	T10018	T10017	prep	of,loss
R2675	T10019	T10018	pobj	p150CAF,of
R2676	T9864	T9862	ccomp	altered,showed
R2677	T10020	T10019	punct	-,p150CAF
R2678	T10021	T10019	nummod	1,p150CAF
R2679	T10022	T10016	prep	to,leads
R2680	T10023	T10022	pobj	disruption,to
R2681	T9865	T9866	det	the,localization
R2682	T10024	T10023	prep	of,disruption
R2683	T10025	T10026	compound	heterochromatin,organization
R2684	T10026	T10024	pobj	organization,of
R2685	T9866	T9864	nsubjpass	localization,altered
R2686	T10027	T10016	advmod	independently,leads
R2687	T10028	T10027	prep	of,independently
R2688	T10029	T10030	det	the,degree
R2689	T9867	T9866	amod	nuclear,localization
R2690	T10030	T10028	pobj	degree,of
R2691	T10031	T10030	prep	of,degree
R2692	T10032	T10033	compound	DNA,methylation
R2693	T10033	T10031	pobj	methylation,of
R2694	T10034	T10030	prep	at,degree
R2695	T9868	T9866	prep	of,localization
R2696	T10035	T10036	amod	pericentric,repeats
R2697	T10036	T10034	pobj	repeats,at
R2698	T10037	T10038	punct	(,data
R2699	T10038	T10014	meta	data,confirmed
R2700	T10039	T10038	amod	unpublished,data
R2701	T10040	T10038	punct	),data
R2702	T10041	T9975	punct	.,tested
R2703	T10043	T10044	nsubj	We,analyzed
R2704	T9869	T9868	pobj	HP1α,of
R2705	T10045	T10044	advmod	next,analyzed
R2706	T10046	T10047	amod	specific,modifications
R2707	T10047	T10044	dobj	modifications,analyzed
R2708	T10048	T10047	compound	histone,modifications
R2709	T10049	T10050	punct	[,27
R2710	T9870	T9864	auxpass	is,altered
R2711	T10050	T10047	parataxis	27,modifications
R2712	T10051	T10050	punct	],27
R2713	T10052	T10044	prep	in,analyzed
R2714	T9871	T9864	advmod	severely,altered
R2715	T10053	T10054	nmod	chromatin,immunoprecipitation
R2716	T10054	T10055	nmod	immunoprecipitation,experiments
R2717	T10055	T10052	pobj	experiments,in
R2718	T9872	T9864	prep	in,altered
R2719	T10056	T10054	punct	(,immunoprecipitation
R2720	T10057	T10054	appos	ChIP,immunoprecipitation
R2721	T10058	T10055	punct	),experiments
R2722	T10059	T10055	acl	using,experiments
R2723	T9873	T9874	nmod	Chaf1a,embryos
R2724	T10060	T10061	amod	native,chromatin
R2725	T10061	T10059	dobj	chromatin,using
R2726	T9874	T9872	pobj	embryos,in
R2727	T10062	T10063	punct	[,28
R2728	T10063	T10044	parataxis	28,analyzed
R2729	T9875	T9873	punct	−,Chaf1a
R2730	T10064	T10063	punct	],28
R2731	T9876	T9873	punct	/,Chaf1a
R2732	T10065	T10044	punct	.,analyzed
R2733	T10067	T10068	nsubj	We,found
R2734	T9877	T9873	punct	−,Chaf1a
R2735	T10069	T10070	mark	that,immunoprecipitated
R2736	T10070	T10068	ccomp	immunoprecipitated,found
R2737	T9878	T9874	cc	and,embryos
R2738	T10071	T10072	amod	pericentric,DNA
R2739	T10072	T10070	nsubjpass	DNA,immunoprecipitated
R2740	T10073	T10070	auxpass	was,immunoprecipitated
R2741	T10074	T10070	prep	with,immunoprecipitated
R2742	T9879	T9880	npadvmod	p150CAF,depleted
R2743	T10075	T10076	det	a,efficiency
R2744	T10076	T10074	pobj	efficiency,with
R2745	T9880	T9884	amod	depleted,cells
R2746	T10077	T10078	npadvmod	2,lower
R2747	T10078	T10076	amod	lower,efficiency
R2748	T10079	T10077	punct	-,2
R2749	T10080	T10077	advmod	fold,2
R2750	T9881	T9879	punct	-,p150CAF
R2751	T10081	T10070	prep	with,immunoprecipitated
R2752	T10082	T10083	nmod	H4K20me3,antibodies
R2753	T10083	T10081	pobj	antibodies,with
R2754	T10084	T10085	punct	[,30
R2755	T10085	T10082	parataxis	30,H4K20me3
R2756	T10086	T10085	nummod	29,30
R2757	T10087	T10085	punct	",",30
R2758	T9882	T9879	nummod	1,p150CAF
R2759	T10088	T10085	punct	],30
R2760	T10089	T10070	prep	following,immunoprecipitated
R2761	T10090	T10091	nmod	p150CAF,depletion
R2762	T9883	T9880	punct	-,depleted
R2763	T10091	T10089	pobj	depletion,following
R2764	T10092	T10090	punct	-,p150CAF
R2765	T10093	T10090	nummod	1,p150CAF
R2766	T9884	T9874	conj	cells,embryos
R2767	T10094	T10095	punct	(,7A
R2768	T10095	T10068	parataxis	7A,found
R2769	T10096	T10095	compound	Figure,7A
R2770	T10097	T10095	cc	and,7A
R2771	T9885	T9884	compound	ES,cells
R2772	T10098	T10095	conj	7B,7A
R2773	T10099	T10095	punct	),7A
R2774	T10100	T10068	punct	.,found
R2775	T9886	T9862	punct	.,showed
R2776	T10102	T10103	nsubjpass	Immunoprecipitation,affected
R2777	T10104	T10102	prep	of,Immunoprecipitation
R2778	T9888	T9889	nsubj	We,examined
R2779	T10105	T10106	amod	minor,repeats
R2780	T10106	T10104	pobj	repeats,of
R2781	T10107	T10106	compound	satellite,repeats
R2782	T10108	T10102	prep	in,Immunoprecipitation
R2783	T10109	T10110	det	the,experiment
R2784	T9890	T9889	advmod	therefore,examined
R2785	T10110	T10108	pobj	experiment,in
R2786	T10111	T10110	amod	same,experiment
R2787	T10112	T10103	auxpass	was,affected
R2788	T10113	T10103	advmod	less,affected
R2789	T10114	T10115	punct	(,7B
R2790	T9891	T9892	det	the,status
R2791	T10115	T10103	parataxis	7B,affected
R2792	T10116	T10115	compound	Figure,7B
R2793	T10117	T10115	punct	),7B
R2794	T9892	T9889	dobj	status,examined
R2795	T10118	T10103	punct	", ",affected
R2796	T10119	T10103	advcl	showing,affected
R2797	T10120	T10121	mark	that,affects
R2798	T9893	T9892	prep	of,status
R2799	T10121	T10119	ccomp	affects,showing
R2800	T9894	T9895	amod	other,marks
R2801	T9895	T9893	pobj	marks,of
R2802	T9896	T9895	amod	epigenetic,marks
R2803	T10122	T10121	nsubj	loss,affects
R2804	T10123	T10122	prep	of,loss
R2805	T10124	T10125	nmod	p150CAF,function
R2806	T10125	T10123	pobj	function,of
R2807	T10126	T10124	punct	-,p150CAF
R2808	T9897	T9898	advmod	previously,shown
R2809	T10127	T10124	nummod	1,p150CAF
R2810	T10128	T10121	advmod	mainly,affects
R2811	T10129	T10121	dobj	H4K20me3,affects
R2812	T10130	T10129	prep	at,H4K20me3
R2813	T9898	T9895	acl	shown,marks
R2814	T10131	T10132	amod	pericentric,heterochromatin
R2815	T9899	T9900	aux	to,characterize
R2816	T10132	T10130	pobj	heterochromatin,at
R2817	T9900	T9898	xcomp	characterize,shown
R2818	T10133	T10103	punct	.,affected
R2819	T9901	T9902	amod	pericentric,heterochromatin
R2820	T10135	T10136	advmod	Moreover,detected
R2821	T9902	T9900	dobj	heterochromatin,characterize
R2822	T10137	T10136	punct	", ",detected
R2823	T10138	T10139	det	no,difference
R2824	T9903	T9889	punct	.,examined
R2825	T10139	T10136	nsubjpass	difference,detected
R2826	T10140	T10139	amod	significant,difference
R2827	T10141	T10139	prep	in,difference
R2828	T9905	T9906	nsubj	We,tested
R2829	T10142	T10143	compound	immunoprecipitation,efficiency
R2830	T10143	T10141	pobj	efficiency,in
R2831	T10144	T10136	auxpass	was,detected
R2832	T10145	T10136	prep	at,detected
R2833	T9907	T9906	advmod	first,tested
R2834	T10146	T10147	amod	intracisternal,particle
R2835	T10147	T10149	nmod	particle,elements
R2836	T10148	T10147	nmod	A,particle
R2837	T9908	T9909	compound	DNA,methylation
R2838	T10149	T10145	pobj	elements,at
R2839	T10150	T10147	punct	(,particle
R2840	T9909	T9906	dobj	methylation,tested
R2841	T10151	T10147	appos	IAP,particle
R2842	T10152	T10149	punct	),elements
R2843	T10153	T10154	punct	(,7B
R2844	T9910	T9909	compound	CpG,methylation
R2845	T10154	T10149	parataxis	7B,elements
R2846	T10155	T10154	compound	Figure,7B
R2847	T10156	T10154	punct	),7B
R2848	T9911	T9912	punct	[,25
R2849	T10157	T10149	punct	", ",elements
R2850	T10158	T10159	dep	which,are
R2851	T10159	T10149	relcl	are,elements
R2852	T10227	T10224	conj	7B,7A
R2853	T10160	T10161	amod	noncentromeric,elements
R2854	T10161	T10159	attr	elements,are
R2855	T10162	T10161	amod	repeated,elements
R2856	T10163	T10161	compound	DNA,elements
R2857	T10164	T10165	advmod	also,enriched
R2858	T10165	T10161	acl	enriched,elements
R2859	T10166	T10165	prep	in,enriched
R2860	T10167	T10166	pobj	H4K20me3,in
R2861	T10168	T10169	punct	[,31
R2862	T10228	T10224	punct	),7A
R2863	T10169	T10136	parataxis	31,detected
R2864	T10170	T10169	punct	],31
R2865	T10171	T10136	punct	.,detected
R2866	T10229	T10201	punct	.,reduced
R2867	T10173	T10174	det	This,result
R2868	T10174	T10175	nsubj	result,shows
R2869	T10231	T10232	prep	In,are
R2870	T10176	T10177	mark	that,contributes
R2871	T10177	T10175	ccomp	contributes,shows
R2872	T10178	T10177	nsubj	p150CAF,contributes
R2873	T10179	T10178	punct	-,p150CAF
R2874	T10233	T10234	amod	wild,type
R2875	T10180	T10178	nummod	1,p150CAF
R2876	T10181	T10177	prep	to,contributes
R2877	T10182	T10183	amod	normal,levels
R2878	T10183	T10181	pobj	levels,to
R2879	T10234	T10236	compound	type,cells
R2880	T10184	T10183	prep	of,levels
R2881	T10185	T10184	pobj	H4K20me3,of
R2882	T10186	T10177	prep	at,contributes
R2883	T10235	T10234	punct	-,type
R2884	T10187	T10188	amod	pericentric,heterochromatin
R2885	T10188	T10186	pobj	heterochromatin,at
R2886	T10189	T10177	punct	", ",contributes
R2887	T10190	T10177	cc	but,contributes
R2888	T10236	T10231	pobj	cells,In
R2889	T10191	T10192	neg	not,necessarily
R2890	T10192	T10177	conj	necessarily,contributes
R2891	T10237	T10232	punct	", ",are
R2892	T10193	T10192	prep	at,necessarily
R2893	T10194	T10195	amod	other,loci
R2894	T10195	T10193	pobj	loci,at
R2895	T10238	T10232	nsubj	most,are
R2896	T10196	T10175	punct	.,shows
R2897	T10198	T10199	det	The,mark
R2898	T10199	T10201	nsubjpass	mark,reduced
R2899	T10200	T10199	compound	H3K9me3,mark
R2900	T10239	T10238	prep	of,most
R2901	T10202	T10203	punct	[,32
R2902	T10203	T10199	parataxis	32,mark
R2903	T10240	T10241	nmod	H4K20me3,signals
R2904	T10204	T10203	punct	],32
R2905	T10205	T10201	auxpass	was,reduced
R2906	T10206	T10201	advmod	also,reduced
R2907	T10241	T10239	pobj	signals,of
R2908	T10207	T10201	advmod	significantly,reduced
R2909	T10208	T10201	prep	in,reduced
R2910	T10209	T10210	npadvmod	p150CAF,depleted
R2911	T10210	T10214	amod	depleted,cells
R2912	T10211	T10209	punct	-,p150CAF
R2913	T10212	T10209	nummod	1,p150CAF
R2914	T10213	T10210	punct	-,depleted
R2915	T10242	T10240	cc	and,H4K20me3
R2916	T10214	T10208	pobj	cells,in
R2917	T10215	T10201	punct	", ",reduced
R2918	T10216	T10217	mark	though,to
R2919	T10217	T10201	advcl	to,reduced
R2920	T10243	T10240	conj	H3K9me3,H4K20me3
R2921	T10218	T10219	det	a,extent
R2922	T10219	T10217	pobj	extent,to
R2923	T10220	T10219	amod	lesser,extent
R2924	T10244	T10232	acomp	present,are
R2925	T10221	T10219	prep	than,extent
R2926	T10222	T10221	pobj	H4K20me3,than
R2927	T10223	T10224	punct	(,7A
R2928	T10245	T10232	prep	at,are
R2929	T10224	T10201	parataxis	7A,reduced
R2930	T10225	T10224	compound	Figure,7A
R2931	T10226	T10224	cc	and,7A
R2932	T10246	T10247	det	the,level
R2933	T10247	T10245	pobj	level,at
R2934	T10248	T10247	prep	of,level
R2935	T10249	T10250	npadvmod	DAPI,dense
R2937	T10250	T10252	amod	dense,domains
R2939	T10251	T10250	punct	-,dense
R2943	T10252	T10248	pobj	domains,of
R2946	T10253	T10254	advmod	when,visualized
R2950	T10254	T10232	advcl	visualized,are
R2955	T10255	T10254	prep	by,visualized
R2958	T10256	T10255	pobj	IF,by
R2965	T10257	T10258	punct	[,29
R2969	T10258	T10254	parataxis	29,visualized
R2972	T10361	T10362	compound	ES,cells
R2973	T10362	T10360	pobj	cells,in
R2974	T10259	T10258	punct	],29
R2975	T10363	T10354	prep	to,leads
R2976	T10364	T10365	det	a,disruption
R2977	T10365	T10363	pobj	disruption,to
R2978	T10260	T10261	punct	(,7C
R2979	T10366	T10365	amod	simultaneous,disruption
R2980	T10367	T10365	prep	of,disruption
R2981	T10368	T10369	nmod	heterochromatin,organization
R2982	T10261	T10232	parataxis	7C,are
R2983	T10369	T10367	pobj	organization,of
R2984	T10370	T10371	nummod	3,D
R2985	T10371	T10369	compound	D,organization
R2986	T10262	T10261	compound	Figures,7C
R2987	T10372	T10371	punct	-,D
R2988	T10373	T10365	cc	and,disruption
R2989	T10374	T10375	det	an,alteration
R2990	T10375	T10365	conj	alteration,disruption
R2991	T10263	T10261	cc	and,7C
R2992	T10376	T10375	prep	in,alteration
R2993	T10377	T10378	amod	epigenetic,marking
R2994	T10264	T10261	conj	7D,7C
R2995	T10378	T10376	pobj	marking,in
R2996	T10379	T10349	punct	.,show
R2997	T10265	T10261	punct	),7C
R2998	T10266	T10232	punct	.,are
R2999	T10268	T10269	nsubjpass	H3K9me3,perturbed
R3000	T10270	T10269	auxpass	was,perturbed
R3001	T10271	T10269	advmod	severely,perturbed
R3002	T10272	T10269	prep	in,perturbed
R3003	T10273	T10274	npadvmod	p150CAF,depleted
R3004	T10274	T10278	amod	depleted,cells
R3005	T10275	T10273	punct	-,p150CAF
R3006	T10276	T10273	nummod	1,p150CAF
R3007	T10277	T10274	punct	-,depleted
R3008	T10278	T10272	pobj	cells,in
R3009	T10279	T10269	punct	", ",perturbed
R3010	T10280	T10269	advcl	displaying,perturbed
R3011	T10281	T10282	det	a,pattern
R3012	T10282	T10280	dobj	pattern,displaying
R3013	T10283	T10282	amod	diffuse,pattern
R3014	T10284	T10282	amod	similar,pattern
R3015	T10285	T10284	prep	to,similar
R3016	T10286	T10285	pobj	HP1α,to
R3017	T10287	T10288	punct	(,7C
R3018	T10288	T10269	parataxis	7C,perturbed
R3019	T10289	T10288	compound	Figure,7C
R3020	T10290	T10288	punct	),7C
R3021	T10291	T10269	punct	.,perturbed
R3022	T10293	T10294	det	The,pattern
R3023	T10294	T10296	nsubjpass	pattern,altered
R3024	T10295	T10294	compound	H4K20me3,pattern
R3025	T10297	T10296	auxpass	was,altered
R3026	T10298	T10299	advmod	even,more
R3027	T10299	T10300	advmod	more,severely
R3028	T10300	T10296	advmod	severely,altered
R3029	T10301	T10296	punct	", ",altered
R3030	T10302	T10296	advcl	showing,altered
R3031	T10303	T10304	preconj	both,loss
R3032	T10304	T10302	dobj	loss,showing
R3033	T10305	T10304	det	a,loss
R3034	T10306	T10304	prep	of,loss
R3035	T10307	T10308	det	the,dots
R3036	T10308	T10306	pobj	dots,of
R3037	T10309	T10308	amod	typical,dots
R3038	T10310	T10308	acl	revealed,dots
R3039	T10311	T10310	prep	in,revealed
R3040	T10312	T10313	compound	control,cells
R3041	T10313	T10311	pobj	cells,in
R3042	T10314	T10304	cc	and,loss
R3043	T10315	T10316	det	a,reduction
R3044	T10316	T10304	conj	reduction,loss
R3045	T10317	T10316	prep	in,reduction
R3046	T10318	T10319	compound	signal,intensity
R3047	T10319	T10317	pobj	intensity,in
R3048	T10320	T10321	punct	(,7D
R3049	T10321	T10296	parataxis	7D,altered
R3050	T10322	T10321	compound	Figure,7D
R3051	T10323	T10321	punct	),7D
R3052	T10324	T10296	punct	.,altered
R3053	T10326	T10327	advcl	Consistent,detected
R3054	T10328	T10326	prep	with,Consistent
R3055	T10329	T10330	poss	our,experiments
R3056	T10330	T10328	pobj	experiments,with
R3057	T10331	T10330	compound	ChIP,experiments
R2936	T10332	T10327	punct	", ",detected
R2938	T10333	T10334	amod	residual,epitopes
R2940	T10334	T10327	nsubjpass	epitopes,detected
R2941	T10335	T10334	compound	H4K20me3,epitopes
R2942	T10336	T10327	auxpass	were,detected
R2944	T10337	T10327	prep	at,detected
R2945	T10338	T10339	det	the,level
R2947	T10339	T10337	pobj	level,at
R2948	T10340	T10339	prep	of,level
R2949	T10341	T10342	amod	relocated,material
R2951	T10342	T10340	pobj	material,of
R2952	T10343	T10344	npadvmod	DAPI,dense
R2953	T10344	T10342	amod	dense,material
R2954	T10345	T10344	punct	-,dense
R2956	T10346	T10327	punct	.,detected
R2957	T10348	T10349	advmod	Altogether,show
R2959	T10350	T10349	punct	", ",show
R2960	T10351	T10352	poss	our,results
R2961	T10352	T10349	nsubj	results,show
R2962	T10353	T10354	mark	that,leads
R2963	T10354	T10349	ccomp	leads,show
R2964	T10355	T10354	nsubj	depletion,leads
R2966	T10356	T10355	prep	of,depletion
R2967	T10357	T10356	pobj	CAF,of
R2968	T10358	T10357	punct	-,CAF
R2970	T10359	T10357	nummod	1,CAF
R2971	T10360	T10355	prep	in,depletion

craft-ca-core-ex-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id	Subject	Object	Predicate	Lexical cue
T7538	16-20	SO_EXT:0000704	denotes	gene
T7539	34-42	CL:0002322	denotes	ES cells
T7540	37-42	CL_GO_EXT:cell	denotes	cells
T7541	46-52	SO_EXT:sequence_deletion_process	denotes	delete
T7542	53-57	SO_EXT:0000147	denotes	exon
T7543	67-73	PR_EXT:000005402	denotes	Chaf1a
T7544	74-78	SO_EXT:0000704	denotes	gene
T7545	86-93	SO_EXT:sequence_coding_function	denotes	encodes
T7546	94-103	PR_EXT:000005402	denotes	p150CAF-1
T7547	117-123	PR_EXT:000005402	denotes	Chaf1a
T7548	124-125	SO_EXT:normal_or_wild_type_or_present	denotes	+
T7549	126-127	SO_EXT:sequence_nullness_or_absence	denotes	−
T7550	128-132	NCBITaxon:10088	denotes	mice
T7551	138-142	GO:0007567	denotes	born
T7552	277-281	NCBITaxon:10088	denotes	mice
T7553	308-315	PATO_UBERON_EXT:neonate_or_newborn	denotes	newborn
T7554	316-322	PR_EXT:000005402	denotes	Chaf1a
T7555	323-324	SO_EXT:sequence_nullness_or_absence	denotes	−
T7556	325-326	SO_EXT:sequence_nullness_or_absence	denotes	−
T7557	327-331	NCBITaxon:10088	denotes	mice
T7558	360-366	PR_EXT:000005402	denotes	Chaf1a
T7559	367-368	SO_EXT:sequence_nullness_or_absence	denotes	−
T7560	369-370	SO_EXT:sequence_nullness_or_absence	denotes	−
T7561	371-378	UBERON:0000922	denotes	embryos
T7562	412-424	GO:0007566	denotes	implantation
T7563	458-464	PR_EXT:000005402	denotes	Chaf1a
T7564	465-466	SO_EXT:sequence_nullness_or_absence	denotes	−
T7565	467-468	SO_EXT:sequence_nullness_or_absence	denotes	−
T7566	469-476	UBERON:0000922	denotes	embryos
T7567	480-489	UBERON:0000922	denotes	embryonic
T7568	565-572	UBERON:0000922	denotes	embryos
T7569	587-593	PR_EXT:000005402	denotes	Chaf1a
T7570	594-595	SO_EXT:normal_or_wild_type_or_present	denotes	+
T7571	596-597	SO_EXT:sequence_nullness_or_absence	denotes	−
T7572	598-602	NCBITaxon:10088	denotes	mice
T7573	666-672	SO_EXT:sequence_altered_entity_or_alteration_process	denotes	mutant
T7574	673-680	UBERON:0000922	denotes	embryos
T7575	726-732	SO_EXT:sequence_altered_entity_or_alteration_process	denotes	mutant
T7576	733-740	UBERON:0000922	denotes	embryos
T7577	768-773	CL_GO_EXT:cell	denotes	cells
T7578	805-810	CL_GO_EXT:cell	denotes	cells
T7579	824-833	SO_EXT:wild_type_entity_or_quality	denotes	wild-type
T7580	850-861	UBERON:0000358	denotes	blastocysts
T7581	971-980	SO_EXT:wild_type_entity_or_quality	denotes	wild-type
T7582	985-994	PR_EXT:000005402	denotes	p150CAF-1
T7583	1004-1011	UBERON:0000922	denotes	embryos
T7584	1023-1032	PR_EXT:000005402	denotes	p150CAF-1
T7585	1075-1091	UBERON:0007236	denotes	eight-cell stage
T7586	1081-1085	CL_GO_EXT:cell	denotes	cell
T7587	1150-1157	CHEBI_PR_EXT:protein	denotes	protein
T7588	1176-1182	UBERON:0000922	denotes	embryo
T7589	1193-1208	UBERON:0007233	denotes	four-cell stage
T7590	1198-1202	CL_GO_EXT:cell	denotes	cell
T7591	1229-1238	PR_EXT:000005402	denotes	p150CAF-1
T7592	1239-1246	CHEBI_PR_EXT:protein	denotes	protein
T7593	1298-1302	CL_GO_EXT:cell	denotes	cell
T7594	1298-1312	GO:0051301	denotes	cell divisions
T7595	3934-3941	GO:0005634	denotes	nuclear
T7596	3958-3973	GO:0000792	denotes	heterochromatin
T7597	3999-4005	GO:0005634	denotes	nuclei
T7598	4009-4015	PR_EXT:000005402	denotes	Chaf1a
T7599	4016-4017	SO_EXT:sequence_nullness_or_absence	denotes	−
T7600	4018-4019	SO_EXT:sequence_nullness_or_absence	denotes	−
T7601	4020-4027	UBERON:0000922	denotes	embryos
T7602	4029-4056	GO:0005721	denotes	Pericentric heterochromatin
T7603	4094-4109	GO:0000792	denotes	heterochromatin
T7604	4139-4149	GO:0051325	denotes	interphase
T7605	4150-4156	GO:0005634	denotes	nuclei
T7606	4164-4176	CHEBI_EXT:51217	denotes	fluorochrome
T7607	4177-4181	CHEBI:51231	denotes	DAPI
T7608	4209-4213	PR_EXT:000005086	denotes	HP1α
T7609	4229-4238	SO_EXT:wild_type_entity_or_quality	denotes	wild-type
T7610	4239-4250	UBERON:0000358	denotes	blastocysts
T7611	4258-4263	NCBITaxon:10088	denotes	mouse
T7613	4272-4277	CL_GO_EXT:cell	denotes	cells
T7614	4279-4306	GO:0005721	denotes	pericentric heterochromatin
T7615	4354-4363	GO:0000785	denotes	chromatin
T7616	4382-4395	GO:0010369	denotes	chromocenters
T7617	4426-4430	CHEBI:51231	denotes	DAPI
T7618	4493-4499	PR_EXT:000005402	denotes	Chaf1a
T7619	4500-4501	SO_EXT:sequence_nullness_or_absence	denotes	−
T7620	4502-4503	SO_EXT:sequence_nullness_or_absence	denotes	−
T7621	4504-4511	UBERON:0000922	denotes	embryos
T7622	4531-4540	GO:0000785	denotes	chromatin
T7623	4600-4606	SO_EXT:0001026	denotes	genome
T7624	4724-4739	GO:0000792	denotes	heterochromatin
T7625	4765-4773	_FRAGMENT	denotes	two-cell
T7626	4789-4795	UBERON:0007232	denotes	stages
T7627	4769-4773	CL_GO_EXT:cell	denotes	cell
T7628	4778-4788	UBERON:0000358	denotes	blastocyst
T7629	4800-4814	UBERON:0007232	denotes	two-cell stage
T7630	4804-4808	CL_GO_EXT:cell	denotes	cell
T7631	4815-4824	SO_EXT:wild_type_entity_or_quality	denotes	wild-type
T7632	4825-4832	UBERON:0000922	denotes	embryos
T7633	4834-4838	CHEBI:51231	denotes	DAPI
T7634	4916-4942	GO:0005731	denotes	nucleolar precursor bodies
T7635	4956-4971	GO:0000792	denotes	Heterochromatin
T7636	5013-5017	CHEBI:51231	denotes	DAPI
T7637	5041-5050	_FRAGMENT	denotes	four-cell
T7638	5074-5080	UBERON:0007233	denotes	stages
T7639	5046-5050	CL_GO_EXT:cell	denotes	cell
T7640	5058-5062	CL_GO_EXT:cell	denotes	cell
T7641	5063-5073	UBERON:0000358	denotes	blastocyst
T7642	5110-5114	PR_EXT:000005086	denotes	HP1α
T7643	5159-5163	CHEBI:51231	denotes	DAPI
T7644	5200-5215	GO:0000792	denotes	heterochromatin
T7645	5239-5246	GO:0005634	denotes	nuclear
T7646	5263-5278	GO:0000792	denotes	heterochromatin
T7647	5315-5327	GO:0007566	denotes	implantation
T7648	5353-5361	_FRAGMENT	denotes	two-cell
T7649	5377-5383	UBERON:0007232	denotes	stages
T7650	5357-5361	CL_GO_EXT:cell	denotes	cell
T7651	5366-5376	UBERON:0000358	denotes	blastocyst
T7652	5388-5394	PR_EXT:000005402	denotes	Chaf1a
T7653	5395-5396	SO_EXT:sequence_nullness_or_absence	denotes	−
T7654	5397-5398	SO_EXT:sequence_nullness_or_absence	denotes	−
T7655	5402-5409	UBERON:0000922	denotes	embryos
T7656	5442-5448	_FRAGMENT	denotes	eight-
T7657	5456-5466	UBERON:0007236	denotes	cell stage
T7658	5456-5460	CL_GO_EXT:cell	denotes	cell
T7659	5468-5472	CHEBI:51231	denotes	DAPI
T7660	5529-5533	CHEBI:51231	denotes	DAPI
T7661	5566-5573	GO:0005634	denotes	nucleus
T7662	5617-5625	GO:0005730	denotes	nucleoli
T7663	5637-5649	_FRAGMENT	denotes	periphery of
T7664	5654-5660	GO:0034399	denotes	nuclei
T7665	5674-5686	GO_PATO_EXT:biological_localization_process_or_quality	denotes	Localization
T7666	5690-5694	PR_EXT:000005086	denotes	HP1α
T7667	5735-5739	CHEBI:51231	denotes	DAPI
T7668	5769-5786	GO:0034399	denotes	nuclear periphery
T7669	5803-5811	GO:0005730	denotes	nucleoli
T7670	5855-5870	GO:0000792	denotes	heterochromatin
T7671	5874-5880	PR_EXT:000005402	denotes	Chaf1a
T7672	5881-5882	SO_EXT:sequence_nullness_or_absence	denotes	−
T7673	5883-5884	SO_EXT:sequence_nullness_or_absence	denotes	−
T7674	5885-5892	UBERON:0000922	denotes	embryos
T7675	5915-5930	GO:0000792	denotes	heterochromatin
T7676	5953-5957	_FRAGMENT	denotes	two-
T7677	5966-5976	UBERON:0007232	denotes	cell stage
T7678	5961-5976	UBERON:0007233	denotes	four-cell stage
T7679	5966-5970	CL_GO_EXT:cell	denotes	cell
T7680	5977-5986	SO_EXT:wild_type_entity_or_quality	denotes	wild-type
T7681	5987-5994	UBERON:0000922	denotes	embryos
T7682	6058-6067	PR_EXT:000005402	denotes	p150CAF-1
T7683	6079-6091	GO:0007566	denotes	implantation
T7684	6126-6133	CHEBI_PR_EXT:protein	denotes	protein
T7685	6181-6203	_FRAGMENT	denotes	heterochromatin within
T7686	6219-6225	GO:0005720	denotes	nuclei
T7687	6204-6213	UBERON:0000922	denotes	embryonic
T7688	6204-6218	CL:0002321	denotes	embryonic cell
T7689	6214-6225	GO:0005634	denotes	cell nuclei
T7690	6279-6284	GO:0033186	denotes	CAF-1
T7691	6311-6319	CL:0002322	denotes	ES cells
T7692	6314-6319	CL_GO_EXT:cell	denotes	cells
T7693	6348-6358	UBERON:0000358	denotes	blastocyst
T7694	6359-6374	UBERON:0000087	denotes	inner cell mass
T7695	6365-6369	CL_GO_EXT:cell	denotes	cell
T7696	6425-6431	PR_EXT:000005402	denotes	Chaf1a
T7697	6432-6433	SO_EXT:sequence_nullness_or_absence	denotes	−
T7698	6434-6435	SO_EXT:sequence_nullness_or_absence	denotes	−
T7699	6436-6443	UBERON:0000922	denotes	embryos
T7700	6450-6455	CL_GO_EXT:cell	denotes	cells
T7701	6491-6495	SO_EXT:sequence_nullness	denotes	null
T7702	6496-6503	UBERON:0000922	denotes	embryos
T7703	6525-6529	GO:0016246	denotes	RNAi
T7704	6552-6561	PR_EXT:000005402	denotes	p150CAF-1
T7705	6575-6583	CL:0002322	denotes	ES cells
T7706	6578-6583	CL_GO_EXT:cell	denotes	cells
T7707	6645-6654	PR_EXT:000005402	denotes	p150CAF-1
T7708	6655-6659	GO:0016246	denotes	RNAi
T7709	6669-6677	CL:0002322	denotes	ES cells
T7710	6672-6677	CL_GO_EXT:cell	denotes	cells
T7711	6720-6725	CL_GO_EXT:cell	denotes	cells
T7712	6729-6735	PR_EXT:000005402	denotes	Chaf1a
T7713	6736-6737	SO_EXT:sequence_nullness_or_absence	denotes	−
T7714	6738-6739	SO_EXT:sequence_nullness_or_absence	denotes	−
T7715	6743-6747	NCBITaxon:10088	denotes	mice
T7716	6749-6753	CHEBI:51231	denotes	DAPI
T7717	6800-6804	PR_EXT:000005086	denotes	HP1α
T7718	6809-6813	CHEBI:51231	denotes	DAPI
T7719	6834-6842	GO:0005730	denotes	nucleoli
T7720	6854-6866	_FRAGMENT	denotes	periphery of
T7721	6871-6878	GO:0034399	denotes	nucleus
T7722	6919-6928	PR_EXT:000005402	denotes	p150CAF-1
T7723	6946-6953	GO:0005634	denotes	nuclear
T7724	6970-6985	GO:0000792	denotes	heterochromatin
T7725	6989-6997	CL:0002322	denotes	ES cells
T7726	6992-6997	CL_GO_EXT:cell	denotes	cells
T7727	7028-7040	GO:0007566	denotes	implantation
T7728	7041-7048	UBERON:0000922	denotes	embryos
T7729	7109-7124	GO:0000792	denotes	heterochromatin
T7730	7149-7154	NCBITaxon:10088	denotes	mouse
T7731	7155-7164	UBERON:0000922	denotes	embryonic
T7732	7165-7176	CL:0000057	denotes	fibroblasts
T7733	7194-7203	PR_EXT:000005402	denotes	p150CAF-1
T7734	7217-7221	GO:0016246	denotes	RNAi
T7735	7223-7232	PR_EXT:000005402	denotes	p150CAF-1
T7736	7286-7290	CL_GO_EXT:cell	denotes	cell
T7737	7286-7304	GO:0008283	denotes	cell proliferation
T7738	7361-7376	GO:0000792	denotes	heterochromatin
T7739	7417-7426	PR_EXT:000005402	denotes	p150CAF-1
T7740	7444-7449	CL_GO_EXT:cell	denotes	cells
T7741	7465-7480	GO:0000792	denotes	heterochromatin
T7742	7550-7555	GO:0033186	denotes	CAF-1
T7743	7563-7575	UBERON:0019248	denotes	early embryo
T7744	7580-7588	CL:0002322	denotes	ES cells
T7745	7583-7588	CL_GO_EXT:cell	denotes	cells
T7746	8895-8904	PR_EXT:000005402	denotes	p150CAF-1
T7747	8908-8916	CL:0002322	denotes	ES cells
T7748	8911-8916	CL_GO_EXT:cell	denotes	cells
T7749	8942-8957	GO:0000792	denotes	heterochromatin
T7750	8958-8965	GO:0005634	denotes	nuclear
T7751	9034-9041	GO:0005634	denotes	nuclear
T7752	9092-9100	GO:0042571	denotes	antibody
T7753	9113-9126	CL:0000836	denotes	promyelocytic
T7754	9113-9135	PR_EXT:000026474	denotes	promyelocytic leukemia
T7755	9137-9140	PR_EXT:000026474	denotes	PML
T7756	9142-9149	CHEBI_PR_EXT:protein	denotes	protein
T7757	9175-9178	PR_EXT:000026474	denotes	PML
T7758	9175-9193	GO:0016605	denotes	PML nuclear bodies
T7759	9227-9234	GO:0005634	denotes	nuclear
T7760	9333-9336	PR_EXT:000026474	denotes	PML
T7761	9333-9351	GO:0016605	denotes	PML nuclear bodies
T7762	9372-9381	PR_EXT:000005402	denotes	p150CAF-1
T7763	9391-9399	CL:0002322	denotes	ES cells
T7764	9394-9399	CL_GO_EXT:cell	denotes	cells
T7765	9434-9441	GO:0005634	denotes	nuclear
T7766	9489-9494	GO:0033186	denotes	CAF-1
T7767	9562-9567	GO:0033186	denotes	CAF-1
T7768	9614-9623	GO:0000785	denotes	chromatin
T7769	9638-9646	CHEBI_PR_EXT:protein	denotes	proteins
T7770	9655-9662	CHEBI:15358	denotes	histone
T7771	9655-9665	PR_EXT:000027594	denotes	histone H3
T7772	9670-9674	PR_EXT:000005086	denotes	HP1α
T7773	9694-9699	GO:0033186	denotes	CAF-1
T7774	9728-9741	UBERON:0019248	denotes	early embryos
T7775	9749-9757	CL:0002322	denotes	ES cells
T7776	9752-9757	CL_GO_EXT:cell	denotes	cells
T7777	9793-9808	GO:0000792	denotes	heterochromatin
T7778	9812-9819	GO:0005634	denotes	nuclear
T7779	9863-9872	NCBITaxon:40674	denotes	mammalian
T7780	9873-9877	CL_GO_EXT:cell	denotes	cell
T7781	9896-9901	GO:0033186	denotes	CAF-1
T7782	9927-9934	GO:0051320	denotes	S phase
T7783	9987-9999	GO:0009294	denotes	transfection
T7784	10007-10011	GO:0016246	denotes	RNAi
T7785	10012-10018	SO_EXT:0000440	denotes	vector
T7786	10020-10029	PR_EXT:000005402	denotes	p150CAF-1
T7787	10039-10044	CL_GO_EXT:cell	denotes	cells
T7788	10082-10087	GO:0033186	denotes	CAF-1
T7789	10123-10134	GO:0006260	denotes	replication
T7790	10172-10181	CHEBI_EXT:thymine_nucleobase_or_nucleoside_or_nucleotide_molecular_entity_or_group	denotes	thymidine
T7791	10189-10206	CHEBI:472552	denotes	bromodeoxyuridine
T7792	10208-10212	CHEBI:472552	denotes	BrdU
T7793	10231-10235	PR_EXT:000012421	denotes	PCNA
T7794	10237-10250	GO:0008283	denotes	proliferating
T7795	10237-10271	PR_EXT:000012421	denotes	proliferating cell nuclear antigen
T7796	10251-10255	CL_GO_EXT:cell	denotes	cell
T7797	10264-10271	CHEBI_EXT:59132	denotes	antigen
T7798	10351-10356	CL_GO_EXT:cell	denotes	cells
T7799	10358-10361	CHEBI_SO_EXT:DNA	denotes	DNA
T7800	10358-10373	GO:0006260	denotes	DNA replication
T7801	10414-10429	GO:0000792	denotes	heterochromatin
T7802	10465-10473	CL:0002322	denotes	ES cells
T7803	10468-10473	CL_GO_EXT:cell	denotes	cells
T7804	10483-10494	GO:0008283	denotes	proliferate
T7805	10499-10502	GO:0016265	denotes	die
T7806	10519-10528	PR_EXT:000005402	denotes	p150CAF-1
T7807	10558-10566	CL_GO_EXT:cell	denotes	cellular
T7808	10558-10574	GO:0009987	denotes	cellular process
T7809	10582-10589	UBERON:0000922	denotes	embryos
T7810	10637-10646	PR_EXT:000005402	denotes	p150CAF-1
T7811	10659-10667	CL:0002322	denotes	ES cells
T7812	10662-10667	CL_GO_EXT:cell	denotes	cells
T7813	10672-10685	UBERON:0019248	denotes	early embryos
T7814	10719-10737	_FRAGMENT	denotes	heterochromatin in
T7815	10742-10749	GO:0005720	denotes	nucleus
T7816	10777-10781	CL_GO_EXT:cell	denotes	cell
T7817	10777-10787	GO:0007049	denotes	cell cycle
T7818	12071-12086	GO:0000792	denotes	heterochromatin
T7819	12124-12127	CHEBI_SO_EXT:DNA	denotes	DNA
T7820	12149-12162	GO:0097617	denotes	hybridization
T7821	12236-12263	GO_SO_EXT:centromere_or_centromeric_region_or_centromeric_orientation	denotes	centric chromosomal regions
T7822	12274-12279	NCBITaxon:10088	denotes	mouse
T7823	12335-12352	SO_EXT:satellite_unit_or_region	denotes	satellite repeats
T7824	12385-12397	_FRAGMENT	denotes	domains from
T7825	12408-12419	GO_SO_EXT:chromosomal_part_or_position_or_region_or_site	denotes	chromosomes
T7826	12496-12500	CHEBI:51231	denotes	DAPI
T7827	12519-12529	GO:0051325	denotes	interphase
T7828	12530-12537	GO:0005634	denotes	nucleus
T7829	12653-12662	PR_EXT:000005402	denotes	p150CAF-1
T7830	12663-12667	GO:0016246	denotes	RNAi
T7831	12677-12682	CL_GO_EXT:cell	denotes	cells
T7832	12712-12739	GO:0005721	denotes	pericentric heterochromatin
T7833	12790-12794	CHEBI:51231	denotes	DAPI
T7834	12842-12854	_FRAGMENT	denotes	domains from
T7835	12862-12873	GO_SO_EXT:chromosomal_part_or_position_or_region_or_site	denotes	chromosomes
T7836	12988-12993	CL_GO_EXT:cell	denotes	cells
T7837	13053-13070	GO:0034399	denotes	nuclear periphery
T7838	13093-13097	CHEBI:51231	denotes	DAPI
T7839	13133-13142	PR_EXT:000005402	denotes	p150CAF-1
T7840	13231-13238	GO:0005634	denotes	nucleus
T7841	13307-13311	CHEBI:51231	denotes	DAPI
T7842	13322-13331	SO_EXT:satellite_unit_or_region	denotes	satellite
T7843	13332-13345	GO:0097617	denotes	hybridization
T7844	13357-13366	PR_EXT:000005402	denotes	p150CAF-1
T7845	13376-13381	CL_GO_EXT:cell	denotes	cells
T7846	13431-13458	GO:0005721	denotes	pericentric heterochromatin
T7847	13546-13551	CL_GO_EXT:cell	denotes	cells
T7848	13595-13604	PR_EXT:000005402	denotes	p150CAF-1
T7849	13674-13683	PR_EXT:000005402	denotes	p150CAF-1
T7850	13742-13769	GO:0005721	denotes	pericentric heterochromatin
T7851	15525-15530	GO:0033186	denotes	CAF-1
T7852	15552-15559	CHEBI:15358	denotes	histone
T7853	15552-15559	_FRAGMENT	denotes	histone
T7854	15569-15571	PR_EXT:000008603	denotes	H4
T7855	15588-15591	CHEBI_SO_EXT:DNA	denotes	DNA
T7856	15588-15601	GO:0071897	denotes	DNA synthesis
T7857	15659-15668	GO:0000785	denotes	chromatin
T7858	15708-15719	GO:0000786	denotes	nucleosomal
T7859	15740-15747	GO_PR_EXT:deoxyribonuclease_I	denotes	DNase I
T7860	15764-15772	GO_EXT:0004518	denotes	nuclease
T7861	15857-15866	PR_EXT:000005402	denotes	p150CAF-1
T7862	15876-15884	CL:0002322	denotes	ES cells
T7863	15879-15884	CL_GO_EXT:cell	denotes	cells
T7864	15906-15912	SO_EXT:0001026	denotes	genome
T7865	15913-15922	GO:0000785	denotes	chromatin
T7866	15941-15948	SO_EXT:sequence_repeat_unit_or_region	denotes	repeats
T7867	16027-16034	CHEBI:15358	denotes	histone
T7868	16027-16037	PR_EXT:000027594	denotes	histone H3
T7869	16043-16062	GO:0000790	denotes	chromatin in nuclei
T7870	16077-16082	CL_GO_EXT:cell	denotes	cells
T7871	16083-16094	GO:0009294	denotes	transfected
T7872	16112-16121	PR_EXT:000005402	denotes	p150CAF-1
T7873	16122-16126	GO:0016246	denotes	RNAi
T7874	16127-16142	SO_EXT:0000755	denotes	plasmid vectors
T7875	16144-16150	GO:0005634	denotes	Nuclei
T7876	16169-16176	CHEBI_CHMO_EXT:buffer_solution	denotes	buffers
T7877	16192-16196	CHEBI:24866	denotes	salt
T7878	16232-16236	CHEBI:26710	denotes	NaCl
T7879	16244-16248	CHEBI:26710	denotes	NaCl
T7880	16283-16290	CHEBI:15358	denotes	histone
T7881	16283-16293	PR_EXT:000027594	denotes	histone H3
T7882	16320-16329	GO:0000785	denotes	chromatin
T7883	16367-16376	PR_EXT:000005402	denotes	p150CAF-1
T7884	16386-16394	CL:0002322	denotes	ES cells
T7885	16389-16394	CL_GO_EXT:cell	denotes	cells
T7886	16471-16498	GO:0005721	denotes	pericentric heterochromatin
T7887	16555-16566	GO:0000786	denotes	nucleosomal
T7888	17149-17156	GO:0005634	denotes	nuclear
T7889	17157-17169	GO_PATO_EXT:biological_localization_process_or_quality	denotes	localization
T7890	17173-17177	PR_EXT:000005086	denotes	HP1α
T7891	17201-17207	PR_EXT:000005402	denotes	Chaf1a
T7892	17208-17209	SO_EXT:sequence_nullness_or_absence	denotes	−
T7893	17210-17211	SO_EXT:sequence_nullness_or_absence	denotes	−
T7894	17212-17219	UBERON:0000922	denotes	embryos
T7895	17224-17233	PR_EXT:000005402	denotes	p150CAF-1
T7896	17243-17251	CL:0002322	denotes	ES cells
T7897	17246-17251	CL_GO_EXT:cell	denotes	cells
T7898	17295-17311	SO_EXT:epigenetic_modification_entity	denotes	epigenetic marks
T7899	17306-17311	CHEBI_SO_EXT:molecular_indicator_or_label_or_marker_or_tag	denotes	marks
T7900	17345-17372	GO:0005721	denotes	pericentric heterochromatin
T7901	17390-17393	CHEBI_SO_EXT:DNA	denotes	DNA
T7902	17390-17409	GO:0010424	denotes	DNA CpG methylation
T7903	17398-17409	GO_MOP_EXT:methylation	denotes	methylation
T7904	17424-17441	SO_EXT:satellite_unit_or_region	denotes	satellite repeats
T7905	17498-17507	PR_EXT:000005402	denotes	p150CAF-1
T7906	17517-17522	CL_GO_EXT:cell	denotes	cells
T7907	17547-17554	CL:0002322	denotes	ES cell
T7908	17550-17554	CL_GO_EXT:cell	denotes	cell
T7909	17580-17584	GO:0016246	denotes	RNAi
T7910	17602-17608	PATO_UBERON_EXT:female_or_bearer_of_femaleness	denotes	female
T7911	17614-17618	CL_GO_EXT:cell	denotes	cell
T7912	17625-17628	CHEBI_SO_EXT:DNA	denotes	DNA
T7913	17625-17640	GO:0006306	denotes	DNA methylation
T7914	17629-17640	GO_MOP_EXT:methylation	denotes	methylation
T7915	17670-17675	CL_GO_EXT:cell	denotes	cells
T7916	17719-17730	GO_MOP_EXT:methylation	denotes	methylation
T7917	17746-17753	SO_EXT:sequence_repeat_unit_or_region	denotes	repeats
T7918	17797-17812	GO:0000792	denotes	heterochromatin
T7919	17824-17833	PR_EXT:000005402	denotes	p150CAF-1
T7920	17843-17851	CL:0002322	denotes	ES cells
T7921	17846-17851	CL_GO_EXT:cell	denotes	cells
T7922	17867-17871	GO:0016246	denotes	RNAi
T7923	17872-17886	SO_EXT:0000755	denotes	plasmid vector
T7924	17895-17902	CL:0002322	denotes	ES cell
T7925	17898-17902	CL_GO_EXT:cell	denotes	cell
T7926	17936-17945	PR_EXT:000005402	denotes	p150CAF-1
T7927	17969-17984	GO:0000792	denotes	heterochromatin
T7928	18029-18032	CHEBI_SO_EXT:DNA	denotes	DNA
T7929	18029-18044	GO:0006306	denotes	DNA methylation
T7930	18033-18044	GO_MOP_EXT:methylation	denotes	methylation
T7931	18060-18067	SO_EXT:sequence_repeat_unit_or_region	denotes	repeats
T7932	18114-18121	PR_EXT:000043452	denotes	histone
T7933	18114-18121	CHEBI:15358	denotes	histone
T7934	18114-18135	GO:0016570	denotes	histone modifications
T7935	18122-18135	SO_EXT:sequence_alteration_entity_or_process	denotes	modifications
T7936	18144-18153	GO:0000785	denotes	chromatin
T7937	18206-18215	GO:0000785	denotes	chromatin
T7938	18248-18251	CHEBI_SO_EXT:DNA	denotes	DNA
T7939	18328-18338	GO:0042571	denotes	antibodies
T7940	18349-18358	PR_EXT:000005402	denotes	p150CAF-1
T7941	18418-18435	SO_EXT:satellite_unit_or_region	denotes	satellite repeats
T7942	18511-18520	PR_EXT:000005402	denotes	p150CAF-1
T7943	18557-18584	GO:0005721	denotes	pericentric heterochromatin
T7944	18739-18750	GO_SO_EXT:centromere_or_centromeric_region_or_centromeric_orientation	denotes	centromeric
T7945	18751-18759	SO_EXT:sequence_repeat_unit_or_region	denotes	repeated
T7946	18760-18763	CHEBI_SO_EXT:DNA	denotes	DNA
T7947	18828-18837	PR_EXT:000005402	denotes	p150CAF-1
T7948	18882-18909	GO:0005721	denotes	pericentric heterochromatin
T7949	18950-18957	SO_EXT:0001707	denotes	H3K9me3
T7950	18958-18962	CHEBI_SO_EXT:molecular_indicator_or_label_or_marker_or_tag	denotes	mark
T7951	19002-19011	PR_EXT:000005402	denotes	p150CAF-1
T7952	19021-19026	CL_GO_EXT:cell	denotes	cells
T7953	20837-20846	SO_EXT:wild_type_entity_or_quality	denotes	wild-type
T7954	20847-20852	CL_GO_EXT:cell	denotes	cells
T7955	20875-20882	SO_EXT:0001707	denotes	H3K9me3
T7956	20919-20923	CHEBI:51231	denotes	DAPI
T7957	20986-20993	SO_EXT:0001707	denotes	H3K9me3
T7958	21020-21029	PR_EXT:000005402	denotes	p150CAF-1
T7959	21039-21044	CL_GO_EXT:cell	denotes	cells
T7960	21086-21090	PR_EXT:000005086	denotes	HP1α
T7961	21217-21222	CL_GO_EXT:cell	denotes	cells
T7962	21328-21336	CHEBI_SO_EXT:epitope	denotes	epitopes
T7963	21367-21376	GO_EXT:biological_movement_or_translocation_process	denotes	relocated
T7964	21377-21381	CHEBI:51231	denotes	DAPI
T7965	21445-21450	GO:0033186	denotes	CAF-1
T7966	21454-21462	CL:0002322	denotes	ES cells
T7967	21457-21462	CL_GO_EXT:cell	denotes	cells
T7968	21501-21516	GO:0000792	denotes	heterochromatin
T7969	21555-21573	SO_EXT:epigenetic_modification_entity_or_process	denotes	epigenetic marking
T7970	21566-21573	CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process	denotes	marking
T7612	4264-4277	CL:0002371	denotes	somatic cells
R792	T7721	T7720	_lexicallyChainedTo	nucleus,periphery of
R793	T7626	T7625	_lexicallyChainedTo	stages,two-cell
R794	T7638	T7637	_lexicallyChainedTo	stages,four-cell
R796	T7649	T7648	_lexicallyChainedTo	stages,two-cell
R797	T7657	T7656	_lexicallyChainedTo	cell stage,eight-
R798	T7664	T7663	_lexicallyChainedTo	nuclei,periphery of
R799	T7677	T7676	_lexicallyChainedTo	cell stage,two-
R800	T7686	T7685	_lexicallyChainedTo	nuclei,heterochromatin within
R801	T7815	T7814	_lexicallyChainedTo	nucleus,heterochromatin in
R802	T7825	T7824	_lexicallyChainedTo	chromosomes,domains from
R803	T7835	T7834	_lexicallyChainedTo	chromosomes,domains from
R804	T7854	T7853	_lexicallyChainedTo	H4,histone

2_test

Id	Subject	Object	Predicate	Lexical cue
17083276-15306854-85837803	4215-4217	15306854	denotes	16
17083276-11336697-85837804	4218-4220	11336697	denotes	17
17083276-14532126-85837804	4218-4220	14532126	denotes	17
17083276-15071554-85837804	4218-4220	15071554	denotes	17
17083276-15302854-85837805	4397-4399	15302854	denotes	20
17083276-15598472-85837806	4617-4619	15598472	denotes	21
17083276-15306854-85837807	7495-7497	15306854	denotes	16
17083276-15077145-85837808	9247-9249	15077145	denotes	22
17083276-15306854-85837809	9948-9950	15306854	denotes	16
17083276-14519857-85837810	9951-9953	14519857	denotes	23
17083276-12620223-85837811	9954-9956	12620223	denotes	24
17083276-15302854-85837812	12368-12370	15302854	denotes	20
17083276-1606614-85837813	14082-14084	1606614	denotes	47
17083276-6694730-85837814	14116-14118	6694730	denotes	48
17083276-14718166-85837815	15603-15605	14718166	denotes	11
17083276-16212490-85837816	15606-15608	16212490	denotes	12
17083276-1606614-85837817	17115-17117	1606614	denotes	47
17083276-11782440-85837818	17411-17413	11782440	denotes	25
17083276-16244654-85837819	17677-17679	16244654	denotes	26
17083276-15702071-85837820	18137-18139	15702071	denotes	27
17083276-15273407-85837821	18217-18219	15273407	denotes	28
17083276-15145825-85837822	18321-18323	15145825	denotes	29
17083276-15128874-85837823	18324-18326	15128874	denotes	30
17083276-15678104-85837824	18800-18802	15678104	denotes	31
17083276-11701123-85837825	18964-18966	11701123	denotes	32
17083276-1606614-85837826	19490-19492	1606614	denotes	47
17083276-1606614-85837827	20000-20002	1606614	denotes	47
17083276-8013255-85837828	20168-20170	8013255	denotes	49
17083276-9771701-85837829	20261-20263	9771701	denotes	50
17083276-15145825-85837830	20961-20963	15145825	denotes	29
T85283	4215-4217	15306854	denotes	16
T95548	4218-4220	11336697	denotes	17
T47164	4218-4220	14532126	denotes	17
T89267	4218-4220	15071554	denotes	17
T79104	4397-4399	15302854	denotes	20
T19555	4617-4619	15598472	denotes	21
T8277	7495-7497	15306854	denotes	16
T48667	9247-9249	15077145	denotes	22
T83132	9948-9950	15306854	denotes	16
T39792	9951-9953	14519857	denotes	23
T62977	9954-9956	12620223	denotes	24
T32017	12368-12370	15302854	denotes	20
T91663	14082-14084	1606614	denotes	47
T5011	14116-14118	6694730	denotes	48
T63760	15603-15605	14718166	denotes	11
T93971	15606-15608	16212490	denotes	12
T60830	17115-17117	1606614	denotes	47
T68317	17411-17413	11782440	denotes	25
T4146	17677-17679	16244654	denotes	26
T27479	18137-18139	15702071	denotes	27
T52202	18217-18219	15273407	denotes	28
T4457	18321-18323	15145825	denotes	29
T22200	18324-18326	15128874	denotes	30
T3965	18800-18802	15678104	denotes	31
T41821	18964-18966	11701123	denotes	32
T42866	19490-19492	1606614	denotes	47
T25351	20000-20002	1606614	denotes	47
T15798	20168-20170	8013255	denotes	49
T32061	20261-20263	9771701	denotes	50
T1911	20961-20963	15145825	denotes	29

craft-ca-core-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id	Subject	Object	Predicate	Lexical cue
T7310	5159-5163	CHEBI:51231	denotes	DAPI
T7311	5200-5215	GO:0000792	denotes	heterochromatin
T7312	5239-5246	GO:0005634	denotes	nuclear
T7313	5263-5278	GO:0000792	denotes	heterochromatin
T7314	5315-5327	GO:0007566	denotes	implantation
T7315	5353-5361	_FRAGMENT	denotes	two-cell
T7316	5377-5383	UBERON:0007232	denotes	stages
T7317	5366-5376	UBERON:0000358	denotes	blastocyst
T7318	5388-5394	PR:000005402	denotes	Chaf1a
T7319	5402-5409	UBERON:0000922	denotes	embryos
T7320	5442-5448	_FRAGMENT	denotes	eight-
T7321	5456-5466	UBERON:0007236	denotes	cell stage
T7322	5468-5472	CHEBI:51231	denotes	DAPI
T7241	16-20	SO:0000704	denotes	gene
T7242	34-42	CL:0002322	denotes	ES cells
T7243	53-57	SO:0000147	denotes	exon
T7244	67-73	PR:000005402	denotes	Chaf1a
T7245	74-78	SO:0000704	denotes	gene
T7246	94-103	PR:000005402	denotes	p150CAF-1
T7247	117-123	PR:000005402	denotes	Chaf1a
T7248	128-132	NCBITaxon:10088	denotes	mice
T7249	138-142	GO:0007567	denotes	born
T7250	277-281	NCBITaxon:10088	denotes	mice
T7251	316-322	PR:000005402	denotes	Chaf1a
T7252	327-331	NCBITaxon:10088	denotes	mice
T7253	360-366	PR:000005402	denotes	Chaf1a
T7254	371-378	UBERON:0000922	denotes	embryos
T7255	412-424	GO:0007566	denotes	implantation
T7256	458-464	PR:000005402	denotes	Chaf1a
T7257	469-476	UBERON:0000922	denotes	embryos
T7258	480-489	UBERON:0000922	denotes	embryonic
T7259	565-572	UBERON:0000922	denotes	embryos
T7260	587-593	PR:000005402	denotes	Chaf1a
T7261	598-602	NCBITaxon:10088	denotes	mice
T7262	673-680	UBERON:0000922	denotes	embryos
T7263	733-740	UBERON:0000922	denotes	embryos
T7264	850-861	UBERON:0000358	denotes	blastocysts
T7265	985-994	PR:000005402	denotes	p150CAF-1
T7266	1004-1011	UBERON:0000922	denotes	embryos
T7267	1023-1032	PR:000005402	denotes	p150CAF-1
T7268	1075-1091	UBERON:0007236	denotes	eight-cell stage
T7269	1176-1182	UBERON:0000922	denotes	embryo
T7270	1193-1208	UBERON:0007233	denotes	four-cell stage
T7271	1229-1238	PR:000005402	denotes	p150CAF-1
T7272	1298-1312	GO:0051301	denotes	cell divisions
T7273	3934-3941	GO:0005634	denotes	nuclear
T7274	3958-3973	GO:0000792	denotes	heterochromatin
T7275	3999-4005	GO:0005634	denotes	nuclei
T7276	4009-4015	PR:000005402	denotes	Chaf1a
T7277	4020-4027	UBERON:0000922	denotes	embryos
T7278	4029-4056	GO:0005721	denotes	Pericentric heterochromatin
T7279	4094-4109	GO:0000792	denotes	heterochromatin
T7280	4139-4149	GO:0051325	denotes	interphase
T7281	4150-4156	GO:0005634	denotes	nuclei
T7282	4164-4176	CHEBI:51217	denotes	fluorochrome
T7283	4177-4181	CHEBI:51231	denotes	DAPI
T7284	4209-4213	PR:000005086	denotes	HP1α
T7285	4239-4250	UBERON:0000358	denotes	blastocysts
T7286	4258-4263	NCBITaxon:10088	denotes	mouse
T7287	4264-4277	CL:0002371	denotes	somatic cells
T7288	4279-4306	GO:0005721	denotes	pericentric heterochromatin
T7289	4354-4363	GO:0000785	denotes	chromatin
T7290	4382-4395	GO:0010369	denotes	chromocenters
T7291	4426-4430	CHEBI:51231	denotes	DAPI
T7292	4493-4499	PR:000005402	denotes	Chaf1a
T7293	4504-4511	UBERON:0000922	denotes	embryos
T7294	4531-4540	GO:0000785	denotes	chromatin
T7295	4600-4606	SO:0001026	denotes	genome
T7296	4724-4739	GO:0000792	denotes	heterochromatin
T7297	4765-4773	_FRAGMENT	denotes	two-cell
T7298	4789-4795	UBERON:0007232	denotes	stages
T7299	4778-4788	UBERON:0000358	denotes	blastocyst
T7300	4800-4814	UBERON:0007232	denotes	two-cell stage
T7301	4825-4832	UBERON:0000922	denotes	embryos
T7302	4834-4838	CHEBI:51231	denotes	DAPI
T7303	4916-4942	GO:0005731	denotes	nucleolar precursor bodies
T7304	4956-4971	GO:0000792	denotes	Heterochromatin
T7305	5013-5017	CHEBI:51231	denotes	DAPI
T7306	5041-5050	_FRAGMENT	denotes	four-cell
T7307	5074-5080	UBERON:0007233	denotes	stages
T7308	5063-5073	UBERON:0000358	denotes	blastocyst
T7309	5110-5114	PR:000005086	denotes	HP1α
T7323	5529-5533	CHEBI:51231	denotes	DAPI
T7324	5566-5573	GO:0005634	denotes	nucleus
T7325	5617-5625	GO:0005730	denotes	nucleoli
T7326	5637-5649	_FRAGMENT	denotes	periphery of
T7327	5654-5660	GO:0034399	denotes	nuclei
T7328	5690-5694	PR:000005086	denotes	HP1α
T7329	5735-5739	CHEBI:51231	denotes	DAPI
T7330	5769-5786	GO:0034399	denotes	nuclear periphery
T7331	5803-5811	GO:0005730	denotes	nucleoli
T7332	5855-5870	GO:0000792	denotes	heterochromatin
T7333	5874-5880	PR:000005402	denotes	Chaf1a
T7334	5885-5892	UBERON:0000922	denotes	embryos
T7335	5915-5930	GO:0000792	denotes	heterochromatin
T7336	5953-5957	_FRAGMENT	denotes	two-
T7337	5966-5976	UBERON:0007232	denotes	cell stage
T7338	5961-5976	UBERON:0007233	denotes	four-cell stage
T7339	5987-5994	UBERON:0000922	denotes	embryos
T7340	6058-6067	PR:000005402	denotes	p150CAF-1
T7341	6079-6091	GO:0007566	denotes	implantation
T7342	6181-6203	_FRAGMENT	denotes	heterochromatin within
T7343	6219-6225	GO:0005720	denotes	nuclei
T7344	6204-6213	UBERON:0000922	denotes	embryonic
T7345	6204-6218	CL:0002321	denotes	embryonic cell
T7346	6214-6225	GO:0005634	denotes	cell nuclei
T7347	6279-6284	GO:0033186	denotes	CAF-1
T7348	6311-6319	CL:0002322	denotes	ES cells
T7349	6348-6358	UBERON:0000358	denotes	blastocyst
T7350	6359-6374	UBERON:0000087	denotes	inner cell mass
T7351	6425-6431	PR:000005402	denotes	Chaf1a
T7352	6436-6443	UBERON:0000922	denotes	embryos
T7353	6496-6503	UBERON:0000922	denotes	embryos
T7354	6525-6529	GO:0016246	denotes	RNAi
T7355	6552-6561	PR:000005402	denotes	p150CAF-1
T7356	6575-6583	CL:0002322	denotes	ES cells
T7357	6645-6654	PR:000005402	denotes	p150CAF-1
T7358	6655-6659	GO:0016246	denotes	RNAi
T7359	6669-6677	CL:0002322	denotes	ES cells
T7360	6729-6735	PR:000005402	denotes	Chaf1a
T7361	6743-6747	NCBITaxon:10088	denotes	mice
T7362	6749-6753	CHEBI:51231	denotes	DAPI
T7363	6800-6804	PR:000005086	denotes	HP1α
T7364	6809-6813	CHEBI:51231	denotes	DAPI
T7365	6834-6842	GO:0005730	denotes	nucleoli
T7366	6854-6866	_FRAGMENT	denotes	periphery of
T7367	6871-6878	GO:0034399	denotes	nucleus
T7368	6919-6928	PR:000005402	denotes	p150CAF-1
T7369	6946-6953	GO:0005634	denotes	nuclear
T7370	6970-6985	GO:0000792	denotes	heterochromatin
T7371	6989-6997	CL:0002322	denotes	ES cells
T7372	7028-7040	GO:0007566	denotes	implantation
T7373	7041-7048	UBERON:0000922	denotes	embryos
T7374	7109-7124	GO:0000792	denotes	heterochromatin
T7375	7149-7154	NCBITaxon:10088	denotes	mouse
T7376	7155-7164	UBERON:0000922	denotes	embryonic
T7377	7165-7176	CL:0000057	denotes	fibroblasts
T7378	7194-7203	PR:000005402	denotes	p150CAF-1
T7379	7217-7221	GO:0016246	denotes	RNAi
T7380	7223-7232	PR:000005402	denotes	p150CAF-1
T7381	7286-7304	GO:0008283	denotes	cell proliferation
T7382	7361-7376	GO:0000792	denotes	heterochromatin
T7383	7417-7426	PR:000005402	denotes	p150CAF-1
T7384	7465-7480	GO:0000792	denotes	heterochromatin
T7385	7550-7555	GO:0033186	denotes	CAF-1
T7386	7563-7575	UBERON:0019248	denotes	early embryo
T7387	7580-7588	CL:0002322	denotes	ES cells
T7388	8895-8904	PR:000005402	denotes	p150CAF-1
T7389	8908-8916	CL:0002322	denotes	ES cells
T7390	8942-8957	GO:0000792	denotes	heterochromatin
T7391	8958-8965	GO:0005634	denotes	nuclear
T7392	9034-9041	GO:0005634	denotes	nuclear
T7393	9092-9100	GO:0042571	denotes	antibody
T7394	9113-9126	CL:0000836	denotes	promyelocytic
T7395	9113-9135	PR:000026474	denotes	promyelocytic leukemia
T7396	9137-9140	PR:000026474	denotes	PML
T7397	9175-9178	PR:000026474	denotes	PML
T7398	9175-9193	GO:0016605	denotes	PML nuclear bodies
T7399	9227-9234	GO:0005634	denotes	nuclear
T7400	9333-9336	PR:000026474	denotes	PML
T7401	9333-9351	GO:0016605	denotes	PML nuclear bodies
T7402	9372-9381	PR:000005402	denotes	p150CAF-1
T7403	9391-9399	CL:0002322	denotes	ES cells
T7404	9434-9441	GO:0005634	denotes	nuclear
T7405	9489-9494	GO:0033186	denotes	CAF-1
T7406	9562-9567	GO:0033186	denotes	CAF-1
T7407	9614-9623	GO:0000785	denotes	chromatin
T7408	9655-9662	CHEBI:15358	denotes	histone
T7409	9655-9665	PR:000027594	denotes	histone H3
T7410	9670-9674	PR:000005086	denotes	HP1α
T7411	9694-9699	GO:0033186	denotes	CAF-1
T7412	9728-9741	UBERON:0019248	denotes	early embryos
T7413	9749-9757	CL:0002322	denotes	ES cells
T7414	9793-9808	GO:0000792	denotes	heterochromatin
T7415	9812-9819	GO:0005634	denotes	nuclear
T7416	9863-9872	NCBITaxon:40674	denotes	mammalian
T7417	9896-9901	GO:0033186	denotes	CAF-1
T7418	9927-9934	GO:0051320	denotes	S phase
T7419	9987-9999	GO:0009294	denotes	transfection
T7420	10007-10011	GO:0016246	denotes	RNAi
T7421	10012-10018	SO:0000440	denotes	vector
T7422	10020-10029	PR:000005402	denotes	p150CAF-1
T7423	10082-10087	GO:0033186	denotes	CAF-1
T7424	10123-10134	GO:0006260	denotes	replication
T7425	10189-10206	CHEBI:472552	denotes	bromodeoxyuridine
T7426	10208-10212	CHEBI:472552	denotes	BrdU
T7427	10231-10235	PR:000012421	denotes	PCNA
T7428	10237-10250	GO:0008283	denotes	proliferating
T7429	10237-10271	PR:000012421	denotes	proliferating cell nuclear antigen
T7430	10264-10271	CHEBI:59132	denotes	antigen
T7431	10358-10373	GO:0006260	denotes	DNA replication
T7432	10414-10429	GO:0000792	denotes	heterochromatin
T7433	10465-10473	CL:0002322	denotes	ES cells
T7434	10483-10494	GO:0008283	denotes	proliferate
T7435	10499-10502	GO:0016265	denotes	die
T7436	10519-10528	PR:000005402	denotes	p150CAF-1
T7437	10558-10574	GO:0009987	denotes	cellular process
T7438	10582-10589	UBERON:0000922	denotes	embryos
T7439	10637-10646	PR:000005402	denotes	p150CAF-1
T7440	10659-10667	CL:0002322	denotes	ES cells
T7441	10672-10685	UBERON:0019248	denotes	early embryos
T7442	10719-10737	_FRAGMENT	denotes	heterochromatin in
T7443	10742-10749	GO:0005720	denotes	nucleus
T7444	10777-10787	GO:0007049	denotes	cell cycle
T7445	12071-12086	GO:0000792	denotes	heterochromatin
T7446	12149-12162	GO:0097617	denotes	hybridization
T7447	12274-12279	NCBITaxon:10088	denotes	mouse
T7448	12496-12500	CHEBI:51231	denotes	DAPI
T7449	12519-12529	GO:0051325	denotes	interphase
T7450	12530-12537	GO:0005634	denotes	nucleus
T7451	12653-12662	PR:000005402	denotes	p150CAF-1
T7452	12663-12667	GO:0016246	denotes	RNAi
T7453	12712-12739	GO:0005721	denotes	pericentric heterochromatin
T7454	12790-12794	CHEBI:51231	denotes	DAPI
T7455	13053-13070	GO:0034399	denotes	nuclear periphery
T7456	13093-13097	CHEBI:51231	denotes	DAPI
T7457	13133-13142	PR:000005402	denotes	p150CAF-1
T7458	13231-13238	GO:0005634	denotes	nucleus
T7459	13307-13311	CHEBI:51231	denotes	DAPI
T7460	13332-13345	GO:0097617	denotes	hybridization
T7461	13357-13366	PR:000005402	denotes	p150CAF-1
T7462	13431-13458	GO:0005721	denotes	pericentric heterochromatin
T7463	13595-13604	PR:000005402	denotes	p150CAF-1
T7464	13674-13683	PR:000005402	denotes	p150CAF-1
T7465	13742-13769	GO:0005721	denotes	pericentric heterochromatin
T7466	15525-15530	GO:0033186	denotes	CAF-1
T7467	15552-15559	CHEBI:15358	denotes	histone
T7468	15552-15559	_FRAGMENT	denotes	histone
T7469	15569-15571	PR:000008603	denotes	H4
T7470	15588-15601	GO:0071897	denotes	DNA synthesis
T7471	15659-15668	GO:0000785	denotes	chromatin
T7472	15708-15719	GO:0000786	denotes	nucleosomal
T7473	15857-15866	PR:000005402	denotes	p150CAF-1
T7474	15876-15884	CL:0002322	denotes	ES cells
T7475	15906-15912	SO:0001026	denotes	genome
T7476	15913-15922	GO:0000785	denotes	chromatin
T7477	16027-16034	CHEBI:15358	denotes	histone
T7478	16027-16037	PR:000027594	denotes	histone H3
T7479	16043-16062	GO:0000790	denotes	chromatin in nuclei
T7480	16083-16094	GO:0009294	denotes	transfected
T7481	16112-16121	PR:000005402	denotes	p150CAF-1
T7482	16122-16126	GO:0016246	denotes	RNAi
T7483	16127-16142	SO:0000755	denotes	plasmid vectors
T7484	16144-16150	GO:0005634	denotes	Nuclei
T7485	16192-16196	CHEBI:24866	denotes	salt
T7486	16232-16236	CHEBI:26710	denotes	NaCl
T7487	16244-16248	CHEBI:26710	denotes	NaCl
T7488	16283-16290	CHEBI:15358	denotes	histone
T7489	16283-16293	PR:000027594	denotes	histone H3
T7490	16320-16329	GO:0000785	denotes	chromatin
T7491	16367-16376	PR:000005402	denotes	p150CAF-1
T7492	16386-16394	CL:0002322	denotes	ES cells
T7493	16471-16498	GO:0005721	denotes	pericentric heterochromatin
T7494	16555-16566	GO:0000786	denotes	nucleosomal
T7495	17149-17156	GO:0005634	denotes	nuclear
T7496	17173-17177	PR:000005086	denotes	HP1α
T7497	17201-17207	PR:000005402	denotes	Chaf1a
T7498	17212-17219	UBERON:0000922	denotes	embryos
T7499	17224-17233	PR:000005402	denotes	p150CAF-1
T7500	17243-17251	CL:0002322	denotes	ES cells
T7501	17345-17372	GO:0005721	denotes	pericentric heterochromatin
T7502	17390-17409	GO:0010424	denotes	DNA CpG methylation
T7503	17498-17507	PR:000005402	denotes	p150CAF-1
T7504	17547-17554	CL:0002322	denotes	ES cell
T7505	17580-17584	GO:0016246	denotes	RNAi
T7506	17625-17640	GO:0006306	denotes	DNA methylation
T7507	17797-17812	GO:0000792	denotes	heterochromatin
T7508	17824-17833	PR:000005402	denotes	p150CAF-1
T7509	17843-17851	CL:0002322	denotes	ES cells
T7510	17867-17871	GO:0016246	denotes	RNAi
T7511	17872-17886	SO:0000755	denotes	plasmid vector
T7512	17895-17902	CL:0002322	denotes	ES cell
T7513	17936-17945	PR:000005402	denotes	p150CAF-1
T7514	17969-17984	GO:0000792	denotes	heterochromatin
T7515	18029-18044	GO:0006306	denotes	DNA methylation
T7516	18114-18121	PR:000043452	denotes	histone
T7517	18114-18121	CHEBI:15358	denotes	histone
T7518	18114-18135	GO:0016570	denotes	histone modifications
T7519	18144-18153	GO:0000785	denotes	chromatin
T7520	18206-18215	GO:0000785	denotes	chromatin
T7521	18328-18338	GO:0042571	denotes	antibodies
T7522	18349-18358	PR:000005402	denotes	p150CAF-1
T7523	18511-18520	PR:000005402	denotes	p150CAF-1
T7524	18557-18584	GO:0005721	denotes	pericentric heterochromatin
T7525	18828-18837	PR:000005402	denotes	p150CAF-1
T7526	18882-18909	GO:0005721	denotes	pericentric heterochromatin
T7527	18950-18957	SO:0001707	denotes	H3K9me3
T7528	19002-19011	PR:000005402	denotes	p150CAF-1
T7529	20875-20882	SO:0001707	denotes	H3K9me3
T7530	20919-20923	CHEBI:51231	denotes	DAPI
T7531	20986-20993	SO:0001707	denotes	H3K9me3
T7532	21020-21029	PR:000005402	denotes	p150CAF-1
T7533	21086-21090	PR:000005086	denotes	HP1α
T7534	21377-21381	CHEBI:51231	denotes	DAPI
T7535	21445-21450	GO:0033186	denotes	CAF-1
T7536	21454-21462	CL:0002322	denotes	ES cells
T7537	21501-21516	GO:0000792	denotes	heterochromatin
R782	T7443	T7442	_lexicallyChainedTo	nucleus,heterochromatin in
R783	T7298	T7297	_lexicallyChainedTo	stages,two-cell
R784	T7307	T7306	_lexicallyChainedTo	stages,four-cell
R785	T7316	T7315	_lexicallyChainedTo	stages,two-cell
R786	T7321	T7320	_lexicallyChainedTo	cell stage,eight-
R787	T7327	T7326	_lexicallyChainedTo	nuclei,periphery of
R788	T7337	T7336	_lexicallyChainedTo	cell stage,two-
R789	T7343	T7342	_lexicallyChainedTo	nuclei,heterochromatin within
R790	T7469	T7468	_lexicallyChainedTo	H4,histone
R791	T7367	T7366	_lexicallyChainedTo	nucleus,periphery of

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