Immunostaining of Radial Cryosections of Wild-Type (+/+) and Heterozygote (+/−) RanBP2 Mice Retinas with Anti-HKI and −Cox11 Antibodies
A1 and B1 represent magnifications of just the distal section of the retina (inner segment subcellular compartment of the photosensory neuron layer). Note the partial delocalization of HKI to the myoid subcompartment of the inner segment of photoreceptor neurons in +/− mice (B1) and decreased levels of HKI in the inner plexiform (synaptic) layer of the retina (A and B). This is reflected by a significant increase and decrease of the mean fluorescence intensity (in arbitrary units) of HKI in the myoid and inner plexiform (synaptic) layers, respectively (E). AU, arbitrary unit; ROS, rod outer segment; RIS, rod inner segment; ONL, outer nuclear layer; OPL, outer plexiform layer, INL, inner nuclear layer; IPL, inner plexiform layer; GCL, ganglion cell layer; E and M are, respectively, the ellipsoid (mitochondria-rich) and myoid compartments of photosensory neurons.
(476 KB PDF)