Antibodies.
Rabbit antisera were raised against the recombinant murine Cox11 (residues 40–275) and affinity-purified under non-denaturing conditions (Stereogene) as previously described [13]. Cox11 antibodies were used at 200 ng/ml for Western analysis. The monoclonal antibody Hsp70 was from Affinity Bioreagents (Golden, Colorado, United States) and antibodies against the KBD (JX2) and ZnF domains of RanBP2 have been described [13,16]. Polyclonal and monoclonal antibodies against Hexokinase I were provided by J. Wilson. The mAb414 was purchased from Abcam (Cambridge, Massachusetts, United States).