In model species such as Drosophila melanogaster, significant effort has gone into providing accurate gene structure annotations, which account for many of the proteins present in the cell. With the abundance of alternative splicing already known to occur, it is likely that evidence for new examples of alternative splicing will continue to grow. Therefore, even with the availability of high quality annotations, there are new variations in gene structure yet to be discovered and non-expression based prediction methods such as ExAlt can be used to search for new cases of alternative splicing.