Supporting Information
Figure S1  Multiple Intestinal Neoplasia in ApcΔ580 Mice
(A) A representative appearance of small intestine of 4-month-old mouse. Scale bar, 1.5 mm.
(B) Histological analysis of longitudinal section of small intestine shown in (A) by H&E staining. Scale bar, 100 μm.
(C) The typical histopathological appearance of an adenoma shown in (B). Scale bar, 20μm.
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Figure S2  Skin Permeability Assay and Neonatal Mice
(A) Skin permeability assay of E18.5 embryos. Both normal (N) and KA mutant (M) embryos can exclude the blue dye at this age, showing no defects in skin barrier function. Note slight pigmentations in pinnnae and “birthmark” in the forehead of mutant embryo (B), which are present at P1 (C), and become prominent as they grow.
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Figure S3  Immunochemical Examination of P13 Normal and Mutant Thymi
(A–E) P13 normal thymus. (F–K) P13 mutant thymus. Stained with K8 (A), (D), (F), (I), K1 (B), (E), (G), (J), and involucrin (C), (H), (K). Scale bars, 100 μm for (A–C), (F–H), (J–K) and 20 μm for (D–E), (I). Note the lack of involucrin staining in normal thymus but varying degree of positive cells in mutant thymus.
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Accession Numbers
The GenBank ((http://www.ncbi.nlm.nih.gov/Genbank) accession numbers for the genes and gene products discussed in this paper are Apc (NM_007462), β-catenin (NM_007614), AXIN 2 (NM_004655), plakoglobin (NM_002230), Asef (NM_015320), kinesin superfamily–associated protein 3 (NM_014970), EB1 (NM_012325), human homolog of Drosophila Discs large (U13897), Lef1 (NM_010703), Dkk1 (NM_010051), K1 (NM_008473), K5 (NM_027011), K6 (NM_008476), K8 (NM_031170), K14 (NM_016958), involucrin (NM_008412), loricrin (NM_008508), and Shh (NM_009170).