PMC:1540739 / 2365-2506 JSONTXT

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{"project":"craft-ca-core-ex-dev","denotations":[{"id":"T2457","span":{"begin":4,"end":5},"obj":"CHEBI_SO_EXT:base"},{"id":"T2458","span":{"begin":7,"end":15},"obj":"SO_EXT:0000155"},{"id":"T2459","span":{"begin":36,"end":41},"obj":"SO_EXT:sequence_cloning_process"},{"id":"T2460","span":{"begin":99,"end":107},"obj":"GO_EXT:breeding"},{"id":"T2461","span":{"begin":108,"end":112},"obj":"NCBITaxon:10088"}],"text":"20 kb) plasmids, often difficult to clone, are required to increase targeting efficiency and (iii) breeding mice to homozygosity and housing "}

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