AlphaA/BKO was generated by cross breeding alphaAKO-127 [22] and alphaBKO-168 mice [23], also in a 129Sv background. These mice also lack the HSPB2 gene product [23]. Lenses examined were from 5 wk (6 lenses) and 54 wk (16 lenses) old mice. All animals were treated in accordance with the Association for Research in Vision and Ophthalmology Statement for the Use of Animals in Ophthalmic and Vision Research.