Table 7  Nonsynonymous SNP in MMU2, 9 and 17 hg modifier candidate genes predicted by SIFT and Polyphen to alter protein function
Gene  MMU  Mbp  AAÄa  B6/CASTb Polyphen  CAST/B6 Polyphen  B6/CAST SIFT  CAST/B6 SIFT
Nmi  2  52.0  R243C  B  B  T  D
Mtx2  2  74.7  T1099M  PSD  PSD  T  D
Ubr1  2  120.6  A1371T  B  B  D  T
Ptpns1  2  129.3  H224N  PBD  PBD  T  T
Ubce7ip5  2  130.3  A314P  PSD  PSD  T  T
2   I366T  B  B  T  D
Plcg1  2  160.5  T1165A  B  B  T  D
Mmp9  2  164.7  A514P  B  B  D  D
2   P639L  PBD  PBD  T  T
Tyk2  9  21.0  R234H  PSD  PSD  T  T
C831S  PSD  PSD  T  T
T1099M  PSD  PSD  T  D
Vav  17  55.5  Q29H  PSD  PBD  T  T
Q53H  PBD  PSD  T  T
Q65H  PBD  PSD  T  D
MMU, mouse chromosome; AA, amino acid; PBD, probably damaging, PSD, possibly damaging; B, Benign; T, tolerated and D, deleterious.
a Amino acid change, first residue listed corresponds to the CAST allele and second corresponds to the B6 allele.
b Indicates which allele was designated as "mutant", i.e. B6/CAST, B6 allele is mutant and CAST is wild type.