We investigated how many anchor points were necessary to enforce a correct alignment of the three core blocks in this test example. As it turned out, it was sufficient to use one single column of the core blocks as anchor points, namely the first column of the third motif. Technically, this can be done by using three anchor points of length one each: anchor point connecting the first position of this core block in sequence 1 with the corresponding position in sequence 2, another anchor connecting sequence 1 with sequence 3 and a third anchor connecting sequence 1 with sequence 4. Although our anchor points enforced the correct alignment only for a single column, most parts of the core blocks were correctly aligned as shown in Figure 4. The BAliBASE sum-of-pairs score of the resulting alignment was 91% while the column score was 90% as 18 out of 20 columns of the core blocks were correctly aligned. As was generally the case for BAliBASE, the DIALIGN score of the (biologically meaningful) anchored alignment was lower than the score of the (biologically wrong) default alignment. The DIALIGN score of the anchored alignment was 9.82 compared with 11.99 for the non-anchored alignment, so here the score of the anchored alignment was around 18 percent below the score of the non-anchored alignment.