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:"arg1Of","subj":"T21832","obj":"T21833"},{"id":"R15017","pred":"arg2Of","subj":"T21834","obj":"T21833"},{"id":"R15018","pred":"arg1Of","subj":"T21834","obj":"T21835"},{"id":"R15019","pred":"arg1Of","subj":"T21834","obj":"T21836"},{"id":"R15020","pred":"arg3Of","subj":"T21837","obj":"T21833"}],"namespaces":[{"prefix":"_base","uri":"http://kmcs.nii.ac.jp/enju/"}],"text":"A, GP led to DNA binding of NFAT, NFκB and NFIL-6. Human PBMC were incubated with medium control or GP (1 and 100 μg) for 1 h at 37°C. Nuclear extracts were incubated with a 32P-labeled NFAT, NFκB, NFIL-6 or NFκB-IL-8 oligonucleotide probe corresponding to the IFNγ, TNFα, IL-6 and IL-8 gene promoters (Table 1). Arrows with the black head indicate migrational location of the NFAT-DNA, NFκB-DNA, NFIL-6-DNA or NFκB-IL-8-DNA complex compared to free probe (no shift). Arrow with the open head indicates a supershift of NFκB-IL-8-DNA-anti-p65 and -p50, respectively. An autoradiogram from a representative experiment is shown (n = 7). B, Decreased DNA binding of NFκB following GP + TSST-1, compared to TSST-1, GP, or TSST-1/GP. Human PBMC were incubated with medium control, LPS, TSST-1, GP (100 μg), GP + TSST-1 and GP + LPS for 1 h at 37°C. An autoradiogram from a representative experiment is shown. Schematic representation of NFκB-DNA binding activity following GP (1 and 100 μg) or TSST-1 treatment (250 ng) as well as simultaneous administration of both compounds and the theoretical value (GP/TSST-1 calc.). Medium control levels were set equal to 1± SEM and significances (* = p \u003c 0.05) are shown with respect to medium control (n = 4)."}

{"project":"bionlp-st-ge-2016-test-proteins","denotations":[{"id":"T21515","span":{"begin":988,"end":994},"obj":"Protein"},{"id":"T21514","span":{"begin":806,"end":812},"obj":"Protein"},{"id":"T21513","span":{"begin":780,"end":786},"obj":"Protein"},{"id":"T21512","span":{"begin":717,"end":723},"obj":"Protein"},{"id":"T21511","span":{"begin":702,"end":708},"obj":"Protein"},{"id":"T21510","span":{"begin":682,"end":688},"obj":"Protein"},{"id":"T21509","span":{"begin":547,"end":550},"obj":"Protein"},{"id":"T21508","span":{"begin":538,"end":541},"obj":"Protein"},{"id":"T21507","span":{"begin":524,"end":528},"obj":"Protein"},{"id":"T21506","span":{"begin":416,"end":420},"obj":"Protein"},{"id":"T21505","span":{"begin":397,"end":403},"obj":"Protein"},{"id":"T21504","span":{"begin":377,"end":381},"obj":"Protein"},{"id":"T21503","span":{"begin":282,"end":286},"obj":"Protein"},{"id":"T21502","span":{"begin":273,"end":277},"obj":"Protein"},{"id":"T21501","span":{"begin":267,"end":271},"obj":"Protein"},{"id":"T21500","span":{"begin":261,"end":265},"obj":"Protein"},{"id":"T21499","span":{"begin":213,"end":217},"obj":"Protein"},{"id":"T21498","span":{"begin":198,"end":204},"obj":"Protein"},{"id":"T21497","span":{"begin":186,"end":190},"obj":"Protein"},{"id":"T21496","span":{"begin":43,"end":49},"obj":"Protein"},{"id":"T21495","span":{"begin":28,"end":32},"obj":"Protein"}],"namespaces":[{"prefix":"_base","uri":"http://bionlp.dbcls.jp/ontology/ge.owl#"}],"text":"A, GP led to DNA binding of NFAT, NFκB and NFIL-6. Human PBMC were incubated with medium control or GP (1 and 100 μg) for 1 h at 37°C. Nuclear extracts were incubated with a 32P-labeled NFAT, NFκB, NFIL-6 or NFκB-IL-8 oligonucleotide probe corresponding to the IFNγ, TNFα, IL-6 and IL-8 gene promoters (Table 1). Arrows with the black head indicate migrational location of the NFAT-DNA, NFκB-DNA, NFIL-6-DNA or NFκB-IL-8-DNA complex compared to free probe (no shift). Arrow with the open head indicates a supershift of NFκB-IL-8-DNA-anti-p65 and -p50, respectively. An autoradiogram from a representative experiment is shown (n = 7). B, Decreased DNA binding of NFκB following GP + TSST-1, compared to TSST-1, GP, or TSST-1/GP. Human PBMC were incubated with medium control, LPS, TSST-1, GP (100 μg), GP + TSST-1 and GP + LPS for 1 h at 37°C. An autoradiogram from a representative experiment is shown. Schematic representation of NFκB-DNA binding activity following GP (1 and 100 μg) or TSST-1 treatment (250 ng) as well as simultaneous administration of both compounds and the theoretical value (GP/TSST-1 calc.). Medium control levels were set equal to 1± SEM and significances (* = p \u003c 0.05) are shown with respect to medium control (n = 4)."}

{"project":"bionlp-st-ge-2016-uniprot","denotations":[{"id":"T21844","span":{"begin":538,"end":541},"obj":"http://www.uniprot.org/uniprot/Q04206"},{"id":"T21843","span":{"begin":273,"end":277},"obj":"http://www.uniprot.org/uniprot/P05231"},{"id":"T21842","span":{"begin":267,"end":271},"obj":"http://www.uniprot.org/uniprot/P01375"},{"id":"T21841","span":{"begin":524,"end":528},"obj":"http://www.uniprot.org/uniprot/P10145"},{"id":"T21840","span":{"begin":416,"end":420},"obj":"http://www.uniprot.org/uniprot/P10145"},{"id":"T21839","span":{"begin":282,"end":286},"obj":"http://www.uniprot.org/uniprot/P10145"},{"id":"T21838","span":{"begin":213,"end":217},"obj":"http://www.uniprot.org/uniprot/P10145"},{"id":"T21853","span":{"begin":1101,"end":1107},"obj":"http://www.uniprot.org/uniprot/P06886"},{"id":"T21852","span":{"begin":988,"end":994},"obj":"http://www.uniprot.org/uniprot/P06886"},{"id":"T21851","span":{"begin":806,"end":812},"obj":"http://www.uniprot.org/uniprot/P06886"},{"id":"T21850","span":{"begin":780,"end":786},"obj":"http://www.uniprot.org/uniprot/P06886"},{"id":"T21849","span":{"begin":717,"end":723},"obj":"http://www.uniprot.org/uniprot/P06886"},{"id":"T21848","span":{"begin":702,"end":708},"obj":"http://www.uniprot.org/uniprot/P06886"},{"id":"T21847","span":{"begin":682,"end":688},"obj":"http://www.uniprot.org/uniprot/P06886"},{"id":"T21846","span":{"begin":547,"end":550},"obj":"http://www.uniprot.org/uniprot/P19838"},{"id":"T21845","span":{"begin":538,"end":541},"obj":"http://www.uniprot.org/uniprot/P21579"}],"namespaces":[{"prefix":"_base","uri":"http://www.uniprot.org/uniprot/"}],"text":"A, GP led to DNA binding of NFAT, NFκB and NFIL-6. Human PBMC were incubated with medium control or GP (1 and 100 μg) for 1 h at 37°C. Nuclear extracts were incubated with a 32P-labeled NFAT, NFκB, NFIL-6 or NFκB-IL-8 oligonucleotide probe corresponding to the IFNγ, TNFα, IL-6 and IL-8 gene promoters (Table 1). Arrows with the black head indicate migrational location of the NFAT-DNA, NFκB-DNA, NFIL-6-DNA or NFκB-IL-8-DNA complex compared to free probe (no shift). Arrow with the open head indicates a supershift of NFκB-IL-8-DNA-anti-p65 and -p50, respectively. An autoradiogram from a representative experiment is shown (n = 7). B, Decreased DNA binding of NFκB following GP + TSST-1, compared to TSST-1, GP, or TSST-1/GP. Human PBMC were incubated with medium control, LPS, TSST-1, GP (100 μg), GP + TSST-1 and GP + LPS for 1 h at 37°C. An autoradiogram from a representative experiment is shown. Schematic representation of NFκB-DNA binding activity following GP (1 and 100 μg) or TSST-1 treatment (250 ng) as well as simultaneous administration of both compounds and the theoretical value (GP/TSST-1 calc.). Medium control levels were set equal to 1± SEM and significances (* = p \u003c 0.05) are shown with respect to medium control (n = 4)."}

{"project":"UBERON-AE","denotations":[{"id":"T21460","span":{"begin":488,"end":492},"obj":"http://purl.obolibrary.org/obo/UBERON_0000033"},{"id":"T21459","span":{"begin":335,"end":339},"obj":"http://purl.obolibrary.org/obo/UBERON_0000033"}],"text":"A, GP led to DNA binding of NFAT, NFκB and NFIL-6. Human PBMC were incubated with medium control or GP (1 and 100 μg) for 1 h at 37°C. Nuclear extracts were incubated with a 32P-labeled NFAT, NFκB, NFIL-6 or NFκB-IL-8 oligonucleotide probe corresponding to the IFNγ, TNFα, IL-6 and IL-8 gene promoters (Table 1). Arrows with the black head indicate migrational location of the NFAT-DNA, NFκB-DNA, NFIL-6-DNA or NFκB-IL-8-DNA complex compared to free probe (no shift). Arrow with the open head indicates a supershift of NFκB-IL-8-DNA-anti-p65 and -p50, respectively. An autoradiogram from a representative experiment is shown (n = 7). B, Decreased DNA binding of NFκB following GP + TSST-1, compared to TSST-1, GP, or TSST-1/GP. Human PBMC were incubated with medium control, LPS, TSST-1, GP (100 μg), GP + TSST-1 and GP + LPS for 1 h at 37°C. An autoradiogram from a representative experiment is shown. Schematic representation of NFκB-DNA binding activity following GP (1 and 100 μg) or TSST-1 treatment (250 ng) as well as simultaneous administration of both compounds and the theoretical value (GP/TSST-1 calc.). Medium control levels were set equal to 1± SEM and significances (* = p \u003c 0.05) are shown with respect to medium control (n = 4)."}

{"project":"GO-MF","denotations":[{"id":"T21527","span":{"begin":273,"end":277},"obj":"http://purl.obolibrary.org/obo/GO_0005138"},{"id":"T21526","span":{"begin":524,"end":528},"obj":"http://purl.obolibrary.org/obo/GO_0005153"},{"id":"T21525","span":{"begin":416,"end":420},"obj":"http://purl.obolibrary.org/obo/GO_0005153"},{"id":"T21524","span":{"begin":282,"end":286},"obj":"http://purl.obolibrary.org/obo/GO_0005153"},{"id":"T21523","span":{"begin":213,"end":217},"obj":"http://purl.obolibrary.org/obo/GO_0005153"},{"id":"T21522","span":{"begin":17,"end":32},"obj":"http://purl.obolibrary.org/obo/GO_0051525"},{"id":"T21521","span":{"begin":940,"end":947},"obj":"http://purl.obolibrary.org/obo/GO_0005488"},{"id":"T21520","span":{"begin":651,"end":658},"obj":"http://purl.obolibrary.org/obo/GO_0005488"},{"id":"T21519","span":{"begin":17,"end":24},"obj":"http://purl.obolibrary.org/obo/GO_0005488"},{"id":"T21518","span":{"begin":936,"end":947},"obj":"http://purl.obolibrary.org/obo/GO_0003677"},{"id":"T21517","span":{"begin":647,"end":658},"obj":"http://purl.obolibrary.org/obo/GO_0003677"},{"id":"T21516","span":{"begin":13,"end":24},"obj":"http://purl.obolibrary.org/obo/GO_0003677"}],"text":"A, GP led to DNA binding of NFAT, NFκB and NFIL-6. Human PBMC were incubated with medium control or GP (1 and 100 μg) for 1 h at 37°C. Nuclear extracts were incubated with a 32P-labeled NFAT, NFκB, NFIL-6 or NFκB-IL-8 oligonucleotide probe corresponding to the IFNγ, TNFα, IL-6 and IL-8 gene promoters (Table 1). Arrows with the black head indicate migrational location of the NFAT-DNA, NFκB-DNA, NFIL-6-DNA or NFκB-IL-8-DNA complex compared to free probe (no shift). Arrow with the open head indicates a supershift of NFκB-IL-8-DNA-anti-p65 and -p50, respectively. An autoradiogram from a representative experiment is shown (n = 7). B, Decreased DNA binding of NFκB following GP + TSST-1, compared to TSST-1, GP, or TSST-1/GP. Human PBMC were incubated with medium control, LPS, TSST-1, GP (100 μg), GP + TSST-1 and GP + LPS for 1 h at 37°C. An autoradiogram from a representative experiment is shown. Schematic representation of NFκB-DNA binding activity following GP (1 and 100 μg) or TSST-1 treatment (250 ng) as well as simultaneous administration of both compounds and the theoretical value (GP/TSST-1 calc.). Medium control levels were set equal to 1± SEM and significances (* = p \u003c 0.05) are shown with respect to medium control (n = 4)."}

{"project":"sentences","denotations":[{"id":"T21469","span":{"begin":1116,"end":1245},"obj":"Sentence"},{"id":"T21468","span":{"begin":903,"end":1115},"obj":"Sentence"},{"id":"T21467","span":{"begin":728,"end":902},"obj":"Sentence"},{"id":"T21466","span":{"begin":634,"end":727},"obj":"Sentence"},{"id":"T21465","span":{"begin":566,"end":633},"obj":"Sentence"},{"id":"T21464","span":{"begin":468,"end":565},"obj":"Sentence"},{"id":"T21463","span":{"begin":313,"end":467},"obj":"Sentence"},{"id":"T21462","span":{"begin":51,"end":312},"obj":"Sentence"},{"id":"T21461","span":{"begin":0,"end":50},"obj":"Sentence"},{"id":"T69","span":{"begin":51,"end":134},"obj":"Sentence"},{"id":"T70","span":{"begin":135,"end":312},"obj":"Sentence"},{"id":"T71","span":{"begin":313,"end":467},"obj":"Sentence"},{"id":"T72","span":{"begin":468,"end":565},"obj":"Sentence"},{"id":"T73","span":{"begin":566,"end":633},"obj":"Sentence"},{"id":"T74","span":{"begin":634,"end":727},"obj":"Sentence"},{"id":"T75","span":{"begin":728,"end":842},"obj":"Sentence"},{"id":"T76","span":{"begin":843,"end":902},"obj":"Sentence"},{"id":"T77","span":{"begin":903,"end":1115},"obj":"Sentence"},{"id":"T78","span":{"begin":1116,"end":1245},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"A, GP led to DNA binding of NFAT, NFκB and NFIL-6. Human PBMC were incubated with medium control or GP (1 and 100 μg) for 1 h at 37°C. Nuclear extracts were incubated with a 32P-labeled NFAT, NFκB, NFIL-6 or NFκB-IL-8 oligonucleotide probe corresponding to the IFNγ, TNFα, IL-6 and IL-8 gene promoters (Table 1). Arrows with the black head indicate migrational location of the NFAT-DNA, NFκB-DNA, NFIL-6-DNA or NFκB-IL-8-DNA complex compared to free probe (no shift). Arrow with the open head indicates a supershift of NFκB-IL-8-DNA-anti-p65 and -p50, respectively. An autoradiogram from a representative experiment is shown (n = 7). B, Decreased DNA binding of NFκB following GP + TSST-1, compared to TSST-1, GP, or TSST-1/GP. Human PBMC were incubated with medium control, LPS, TSST-1, GP (100 μg), GP + TSST-1 and GP + LPS for 1 h at 37°C. An autoradiogram from a representative experiment is shown. Schematic representation of NFκB-DNA binding activity following GP (1 and 100 μg) or TSST-1 treatment (250 ng) as well as simultaneous administration of both compounds and the theoretical value (GP/TSST-1 calc.). Medium control levels were set equal to 1± SEM and significances (* = p \u003c 0.05) are shown with respect to medium control (n = 4)."}

{"project":"simple1","denotations":[{"id":"T21594","span":{"begin":988,"end":994},"obj":"Protein"},{"id":"T21593","span":{"begin":806,"end":812},"obj":"Protein"},{"id":"T21592","span":{"begin":780,"end":786},"obj":"Protein"},{"id":"T21591","span":{"begin":717,"end":723},"obj":"Protein"},{"id":"T21590","span":{"begin":702,"end":708},"obj":"Protein"},{"id":"T21589","span":{"begin":682,"end":688},"obj":"Protein"},{"id":"T21588","span":{"begin":547,"end":550},"obj":"Protein"},{"id":"T21587","span":{"begin":538,"end":541},"obj":"Protein"},{"id":"T21586","span":{"begin":524,"end":528},"obj":"Protein"},{"id":"T21585","span":{"begin":416,"end":420},"obj":"Protein"},{"id":"T21584","span":{"begin":397,"end":403},"obj":"Protein"},{"id":"T21583","span":{"begin":377,"end":381},"obj":"Protein"},{"id":"T21582","span":{"begin":282,"end":286},"obj":"Protein"},{"id":"T21581","span":{"begin":273,"end":277},"obj":"Protein"},{"id":"T21580","span":{"begin":267,"end":271},"obj":"Protein"},{"id":"T21579","span":{"begin":261,"end":265},"obj":"Protein"},{"id":"T21578","span":{"begin":213,"end":217},"obj":"Protein"},{"id":"T21577","span":{"begin":198,"end":204},"obj":"Protein"},{"id":"T21576","span":{"begin":186,"end":190},"obj":"Protein"},{"id":"T21575","span":{"begin":43,"end":49},"obj":"Protein"},{"id":"T21574","span":{"begin":28,"end":32},"obj":"Protein"}],"text":"A, GP led to DNA binding of NFAT, NFκB and NFIL-6. Human PBMC were incubated with medium control or GP (1 and 100 μg) for 1 h at 37°C. Nuclear extracts were incubated with a 32P-labeled NFAT, NFκB, NFIL-6 or NFκB-IL-8 oligonucleotide probe corresponding to the IFNγ, TNFα, IL-6 and IL-8 gene promoters (Table 1). Arrows with the black head indicate migrational location of the NFAT-DNA, NFκB-DNA, NFIL-6-DNA or NFκB-IL-8-DNA complex compared to free probe (no shift). Arrow with the open head indicates a supershift of NFκB-IL-8-DNA-anti-p65 and -p50, respectively. An autoradiogram from a representative experiment is shown (n = 7). B, Decreased DNA binding of NFκB following GP + TSST-1, compared to TSST-1, GP, or TSST-1/GP. Human PBMC were incubated with medium control, LPS, TSST-1, GP (100 μg), GP + TSST-1 and GP + LPS for 1 h at 37°C. An autoradiogram from a representative experiment is shown. Schematic representation of NFκB-DNA binding activity following GP (1 and 100 μg) or TSST-1 treatment (250 ng) as well as simultaneous administration of both compounds and the theoretical value (GP/TSST-1 calc.). Medium control levels were set equal to 1± SEM and significances (* = p \u003c 0.05) are shown with respect to medium control (n = 4)."}

{"project":"BioNLP16_DUT","denotations":[{"id":"T22281","span":{"begin":17,"end":24},"obj":"Binding"},{"id":"T22280","span":{"begin":988,"end":994},"obj":"Protein"},{"id":"T22279","span":{"begin":806,"end":812},"obj":"Protein"},{"id":"T22278","span":{"begin":780,"end":786},"obj":"Protein"},{"id":"T22277","span":{"begin":717,"end":723},"obj":"Protein"},{"id":"T22276","span":{"begin":702,"end":708},"obj":"Protein"},{"id":"T22275","span":{"begin":682,"end":688},"obj":"Protein"},{"id":"T22274","span":{"begin":547,"end":550},"obj":"Protein"},{"id":"T22273","span":{"begin":538,"end":541},"obj":"Protein"},{"id":"T22272","span":{"begin":524,"end":528},"obj":"Protein"},{"id":"T22271","span":{"begin":416,"end":420},"obj":"Protein"},{"id":"T22270","span":{"begin":397,"end":403},"obj":"Protein"},{"id":"T22269","span":{"begin":377,"end":381},"obj":"Protein"},{"id":"T22268","span":{"begin":282,"end":286},"obj":"Protein"},{"id":"T22267","span":{"begin":273,"end":277},"obj":"Protein"},{"id":"T22266","span":{"begin":267,"end":271},"obj":"Protein"},{"id":"T22265","span":{"begin":261,"end":265},"obj":"Protein"},{"id":"T22264","span":{"begin":213,"end":217},"obj":"Protein"},{"id":"T22263","span":{"begin":198,"end":204},"obj":"Protein"},{"id":"T22262","span":{"begin":186,"end":190},"obj":"Protein"},{"id":"T22261","span":{"begin":43,"end":49},"obj":"Protein"},{"id":"T22260","span":{"begin":28,"end":32},"obj":"Protein"}],"relations":[{"id":"R15305","pred":"themeOf","subj":"T22260","obj":"T22281"},{"id":"R15306","pred":"themeOf","subj":"T22261","obj":"T22281"}],"text":"A, GP led to DNA binding of NFAT, NFκB and NFIL-6. Human PBMC were incubated with medium control or GP (1 and 100 μg) for 1 h at 37°C. Nuclear extracts were incubated with a 32P-labeled NFAT, NFκB, NFIL-6 or NFκB-IL-8 oligonucleotide probe corresponding to the IFNγ, TNFα, IL-6 and IL-8 gene promoters (Table 1). Arrows with the black head indicate migrational location of the NFAT-DNA, NFκB-DNA, NFIL-6-DNA or NFκB-IL-8-DNA complex compared to free probe (no shift). Arrow with the open head indicates a supershift of NFκB-IL-8-DNA-anti-p65 and -p50, respectively. An autoradiogram from a representative experiment is shown (n = 7). B, Decreased DNA binding of NFκB following GP + TSST-1, compared to TSST-1, GP, or TSST-1/GP. Human PBMC were incubated with medium control, LPS, TSST-1, GP (100 μg), GP + TSST-1 and GP + LPS for 1 h at 37°C. An autoradiogram from a representative experiment is shown. Schematic representation of NFκB-DNA binding activity following GP (1 and 100 μg) or TSST-1 treatment (250 ng) as well as simultaneous administration of both compounds and the theoretical value (GP/TSST-1 calc.). Medium control levels were set equal to 1± SEM and significances (* = p \u003c 0.05) are shown with respect to medium control (n = 4)."}

{"project":"BioNLP16_Messiy","denotations":[{"id":"T21854","span":{"begin":28,"end":32},"obj":"Protein"},{"id":"T21878","span":{"begin":651,"end":658},"obj":"Binding"},{"id":"T21877","span":{"begin":637,"end":646},"obj":"Negative_regulation"},{"id":"T21876","span":{"begin":17,"end":24},"obj":"Binding"},{"id":"T21875","span":{"begin":6,"end":9},"obj":"Positive_regulation"},{"id":"T21874","span":{"begin":988,"end":994},"obj":"Protein"},{"id":"T21873","span":{"begin":806,"end":812},"obj":"Protein"},{"id":"T21872","span":{"begin":780,"end":786},"obj":"Protein"},{"id":"T21871","span":{"begin":717,"end":723},"obj":"Protein"},{"id":"T21870","span":{"begin":702,"end":708},"obj":"Protein"},{"id":"T21869","span":{"begin":682,"end":688},"obj":"Protein"},{"id":"T21868","span":{"begin":547,"end":550},"obj":"Protein"},{"id":"T21867","span":{"begin":538,"end":541},"obj":"Protein"},{"id":"T21866","span":{"begin":524,"end":528},"obj":"Protein"},{"id":"T21865","span":{"begin":416,"end":420},"obj":"Protein"},{"id":"T21864","span":{"begin":397,"end":403},"obj":"Protein"},{"id":"T21863","span":{"begin":377,"end":381},"obj":"Protein"},{"id":"T21862","span":{"begin":282,"end":286},"obj":"Protein"},{"id":"T21861","span":{"begin":273,"end":277},"obj":"Protein"},{"id":"T21860","span":{"begin":267,"end":271},"obj":"Protein"},{"id":"T21859","span":{"begin":261,"end":265},"obj":"Protein"},{"id":"T21858","span":{"begin":213,"end":217},"obj":"Protein"},{"id":"T21857","span":{"begin":198,"end":204},"obj":"Protein"},{"id":"T21856","span":{"begin":186,"end":190},"obj":"Protein"},{"id":"T21855","span":{"begin":43,"end":49},"obj":"Protein"}],"relations":[{"id":"R15021","pred":"themeOf","subj":"T21854","obj":"T21876"},{"id":"R15022","pred":"themeOf","subj":"T21855","obj":"T21876"},{"id":"R15023","pred":"themeOf","subj":"T21869","obj":"T21878"},{"id":"R15024","pred":"themeOf","subj":"T21876","obj":"T21875"},{"id":"R15025","pred":"themeOf","subj":"T21876","obj":"T21875"},{"id":"R15026","pred":"themeOf","subj":"T21878","obj":"T21877"}],"text":"A, GP led to DNA binding of NFAT, NFκB and NFIL-6. Human PBMC were incubated with medium control or GP (1 and 100 μg) for 1 h at 37°C. Nuclear extracts were incubated with a 32P-labeled NFAT, NFκB, NFIL-6 or NFκB-IL-8 oligonucleotide probe corresponding to the IFNγ, TNFα, IL-6 and IL-8 gene promoters (Table 1). Arrows with the black head indicate migrational location of the NFAT-DNA, NFκB-DNA, NFIL-6-DNA or NFκB-IL-8-DNA complex compared to free probe (no shift). Arrow with the open head indicates a supershift of NFκB-IL-8-DNA-anti-p65 and -p50, respectively. An autoradiogram from a representative experiment is shown (n = 7). B, Decreased DNA binding of NFκB following GP + TSST-1, compared to TSST-1, GP, or TSST-1/GP. Human PBMC were incubated with medium control, LPS, TSST-1, GP (100 μg), GP + TSST-1 and GP + LPS for 1 h at 37°C. An autoradiogram from a representative experiment is shown. Schematic representation of NFκB-DNA binding activity following GP (1 and 100 μg) or TSST-1 treatment (250 ng) as well as simultaneous administration of both compounds and the theoretical value (GP/TSST-1 calc.). Medium control levels were set equal to 1± SEM and significances (* = p \u003c 0.05) are shown with respect to medium control (n = 4)."}

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GP led to DNA binding of NFAT, NFκB and NFIL-6. Human PBMC were incubated with medium control or GP (1 and 100 μg) for 1 h at 37°C. Nuclear extracts were incubated with a 32P-labeled NFAT, NFκB, NFIL-6 or NFκB-IL-8 oligonucleotide probe corresponding to the IFNγ, TNFα, IL-6 and IL-8 gene promoters (Table 1). Arrows with the black head indicate migrational location of the NFAT-DNA, NFκB-DNA, NFIL-6-DNA or NFκB-IL-8-DNA complex compared to free probe (no shift). Arrow with the open head indicates a supershift of NFκB-IL-8-DNA-anti-p65 and -p50, respectively. An autoradiogram from a representative experiment is shown (n = 7). B, Decreased DNA binding of NFκB following GP + TSST-1, compared to TSST-1, GP, or TSST-1/GP. Human PBMC were incubated with medium control, LPS, TSST-1, GP (100 μg), GP + TSST-1 and GP + LPS for 1 h at 37°C. An autoradiogram from a representative experiment is shown. Schematic representation of NFκB-DNA binding activity following GP (1 and 100 μg) or TSST-1 treatment (250 ng) as well as simultaneous administration of both compounds and the theoretical value (GP/TSST-1 calc.). Medium control levels were set equal to 1± SEM and significances (* = p \u003c 0.05) are shown with respect to medium control (n = 4)."}

{"project":"bionlp-st-ge-2016-test-tees","denotations":[{"id":"T21552","span":{"begin":931,"end":939},"obj":"Protein"},{"id":"T21551","span":{"begin":651,"end":658},"obj":"Binding"},{"id":"T21541","span":{"begin":377,"end":385},"obj":"Protein"},{"id":"T21540","span":{"begin":273,"end":277},"obj":"Protein"},{"id":"T21539","span":{"begin":267,"end":271},"obj":"Protein"},{"id":"T21538","span":{"begin":261,"end":265},"obj":"Protein"},{"id":"T21537","span":{"begin":208,"end":233},"obj":"Protein"},{"id":"T21536","span":{"begin":198,"end":204},"obj":"Protein"},{"id":"T21535","span":{"begin":192,"end":196},"obj":"Protein"},{"id":"T21534","span":{"begin":186,"end":190},"obj":"Protein"},{"id":"T21533","span":{"begin":17,"end":24},"obj":"Binding"},{"id":"T21532","span":{"begin":17,"end":24},"obj":"Binding"},{"id":"T21531","span":{"begin":17,"end":24},"obj":"Binding"},{"id":"T21530","span":{"begin":43,"end":49},"obj":"Protein"},{"id":"T21529","span":{"begin":34,"end":38},"obj":"Protein"},{"id":"T21528","span":{"begin":28,"end":32},"obj":"Protein"},{"id":"T21550","span":{"begin":710,"end":712},"obj":"Protein"},{"id":"T21549","span":{"begin":702,"end":708},"obj":"Protein"},{"id":"T21548","span":{"begin":662,"end":666},"obj":"Protein"},{"id":"T21547","span":{"begin":547,"end":550},"obj":"Protein"},{"id":"T21546","span":{"begin":538,"end":541},"obj":"Protein"},{"id":"T21545","span":{"begin":519,"end":537},"obj":"Protein"},{"id":"T21544","span":{"begin":411,"end":432},"obj":"Protein"},{"id":"T21543","span":{"begin":397,"end":407},"obj":"Protein"},{"id":"T21542","span":{"begin":387,"end":395},"obj":"Protein"}],"relations":[{"id":"R14762","pred":"themeOf","subj":"T21528","obj":"T21531"},{"id":"R14763","pred":"themeOf","subj":"T21529","obj":"T21532"},{"id":"R14764","pred":"themeOf","subj":"T21530","obj":"T21533"},{"id":"R14765","pred":"themeOf","subj":"T21548","obj":"T21551"}],"text":"A, GP led to DNA binding of NFAT, NFκB and NFIL-6. Human PBMC were incubated with medium control or GP (1 and 100 μg) for 1 h at 37°C. Nuclear extracts were incubated with a 32P-labeled NFAT, NFκB, NFIL-6 or NFκB-IL-8 oligonucleotide probe corresponding to the IFNγ, TNFα, IL-6 and IL-8 gene promoters (Table 1). Arrows with the black head indicate migrational location of the NFAT-DNA, NFκB-DNA, NFIL-6-DNA or NFκB-IL-8-DNA complex compared to free probe (no shift). Arrow with the open head indicates a supershift of NFκB-IL-8-DNA-anti-p65 and -p50, respectively. An autoradiogram from a representative experiment is shown (n = 7). B, Decreased DNA binding of NFκB following GP + TSST-1, compared to TSST-1, GP, or TSST-1/GP. Human PBMC were incubated with medium control, LPS, TSST-1, GP (100 μg), GP + TSST-1 and GP + LPS for 1 h at 37°C. An autoradiogram from a representative experiment is shown. Schematic representation of NFκB-DNA binding activity following GP (1 and 100 μg) or TSST-1 treatment (250 ng) as well as simultaneous administration of both compounds and the theoretical value (GP/TSST-1 calc.). Medium control levels were set equal to 1± SEM and significances (* = p \u003c 0.05) are shown with respect to medium control (n = 4)."}

{"project":"testone","denotations":[{"id":"T21413","span":{"begin":651,"end":658},"obj":"Binding"},{"id":"T21412","span":{"begin":17,"end":24},"obj":"Binding"},{"id":"T21411","span":{"begin":6,"end":9},"obj":"Positive_regulation"},{"id":"T21410","span":{"begin":988,"end":994},"obj":"Protein"},{"id":"T21409","span":{"begin":806,"end":812},"obj":"Protein"},{"id":"T21408","span":{"begin":780,"end":786},"obj":"Protein"},{"id":"T21407","span":{"begin":717,"end":723},"obj":"Protein"},{"id":"T21406","span":{"begin":702,"end":708},"obj":"Protein"},{"id":"T21405","span":{"begin":682,"end":688},"obj":"Protein"},{"id":"T21404","span":{"begin":547,"end":550},"obj":"Protein"},{"id":"T21403","span":{"begin":538,"end":541},"obj":"Protein"},{"id":"T21402","span":{"begin":524,"end":528},"obj":"Protein"},{"id":"T21401","span":{"begin":416,"end":420},"obj":"Protein"},{"id":"T21400","span":{"begin":397,"end":403},"obj":"Protein"},{"id":"T21399","span":{"begin":377,"end":381},"obj":"Protein"},{"id":"T21398","span":{"begin":282,"end":286},"obj":"Protein"},{"id":"T21397","span":{"begin":273,"end":277},"obj":"Protein"},{"id":"T21396","span":{"begin":267,"end":271},"obj":"Protein"},{"id":"T21395","span":{"begin":261,"end":265},"obj":"Protein"},{"id":"T21394","span":{"begin":213,"end":217},"obj":"Protein"},{"id":"T21393","span":{"begin":198,"end":204},"obj":"Protein"},{"id":"T21392","span":{"begin":186,"end":190},"obj":"Protein"},{"id":"T21391","span":{"begin":43,"end":49},"obj":"Protein"},{"id":"T21390","span":{"begin":28,"end":32},"obj":"Protein"}],"relations":[{"id":"R14746","pred":"themeOf","subj":"T21390","obj":"T21412"},{"id":"R14747","pred":"themeOf","subj":"T21391","obj":"T21412"},{"id":"R14748","pred":"themeOf","subj":"T21405","obj":"T21413"},{"id":"R14749","pred":"themeOf","subj":"T21406","obj":"T21413"},{"id":"R14750","pred":"themeOf","subj":"T21407","obj":"T21413"},{"id":"R14751","pred":"themeOf","subj":"T21412","obj":"T21411"}],"text":"A, GP led to DNA binding of NFAT, NFκB and NFIL-6. Human PBMC were incubated with medium control or GP (1 and 100 μg) for 1 h at 37°C. Nuclear extracts were incubated with a 32P-labeled NFAT, NFκB, NFIL-6 or NFκB-IL-8 oligonucleotide probe corresponding to the IFNγ, TNFα, IL-6 and IL-8 gene promoters (Table 1). Arrows with the black head indicate migrational location of the NFAT-DNA, NFκB-DNA, NFIL-6-DNA or NFκB-IL-8-DNA complex compared to free probe (no shift). Arrow with the open head indicates a supershift of NFκB-IL-8-DNA-anti-p65 and -p50, respectively. An autoradiogram from a representative experiment is shown (n = 7). B, Decreased DNA binding of NFκB following GP + TSST-1, compared to TSST-1, GP, or TSST-1/GP. Human PBMC were incubated with medium control, LPS, TSST-1, GP (100 μg), GP + TSST-1 and GP + LPS for 1 h at 37°C. An autoradiogram from a representative experiment is shown. Schematic representation of NFκB-DNA binding activity following GP (1 and 100 μg) or TSST-1 treatment (250 ng) as well as simultaneous administration of both compounds and the theoretical value (GP/TSST-1 calc.). Medium control levels were set equal to 1± SEM and significances (* = p \u003c 0.05) are shown with respect to medium control (n = 4)."}

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