Id |
Subject |
Object |
Predicate |
Lexical cue |
T15888 |
743-750 |
JJ |
denotes |
minimal |
T15887 |
732-742 |
VBG |
denotes |
containing |
T15886 |
728-731 |
NN |
denotes |
TRE |
T15885 |
726-727 |
DT |
denotes |
a |
T15884 |
721-725 |
IN |
denotes |
from |
T15883 |
713-715 |
NN |
denotes |
E2 |
T15882 |
707-712 |
JJ |
denotes |
human |
T15881 |
716-720 |
NN |
denotes |
cDNA |
T15880 |
705-706 |
DT |
denotes |
a |
T15879 |
697-704 |
VB |
denotes |
express |
T15878 |
694-696 |
TO |
denotes |
to |
T15877 |
681-684 |
VBD |
denotes |
was |
T15876 |
685-693 |
VBN |
denotes |
designed |
T15875 |
667-668 |
HYPH |
denotes |
- |
T15874 |
668-670 |
NN |
denotes |
E2 |
T15873 |
664-667 |
NN |
denotes |
TRE |
T15872 |
671-680 |
NN |
denotes |
transgene |
T15871 |
660-663 |
DT |
denotes |
The |
T15870 |
659-784 |
sentence |
denotes |
The TRE-E2 transgene was designed to express a human E2 cDNA from a TRE containing minimal promoter upon stimulation by tTA. |
T15869 |
658-659 |
. |
denotes |
. |
T15868 |
657-658 |
-RRB- |
denotes |
) |
T15867 |
654-657 |
NN |
denotes |
TRE |
T15866 |
653-654 |
-LRB- |
denotes |
( |
T15865 |
636-644 |
NN |
denotes |
response |
T15864 |
621-635 |
NN |
denotes |
transactivator |
T15863 |
645-652 |
NN |
denotes |
element |
T15862 |
619-620 |
DT |
denotes |
a |
T15861 |
612-618 |
VBP |
denotes |
harbor |
T15860 |
607-611 |
WDT |
denotes |
that |
T15859 |
597-606 |
NNS |
denotes |
promoters |
T15858 |
594-596 |
IN |
denotes |
of |
T15857 |
583-593 |
NN |
denotes |
expression |
T15856 |
572-582 |
VBZ |
denotes |
stimulates |
T15855 |
567-571 |
WDT |
denotes |
that |
T15854 |
546-559 |
NN |
denotes |
transcription |
T15853 |
560-566 |
NN |
denotes |
factor |
T15852 |
544-545 |
DT |
denotes |
a |
T15851 |
542-544 |
, |
denotes |
, |
T15850 |
541-542 |
-RRB- |
denotes |
) |
T15849 |
538-541 |
NN |
denotes |
tTA |
T15848 |
537-538 |
-LRB- |
denotes |
( |
T15847 |
510-511 |
HYPH |
denotes |
- |
T15846 |
511-521 |
VBN |
denotes |
controlled |
T15845 |
498-510 |
NN |
denotes |
tetracycline |
T15844 |
522-536 |
NN |
denotes |
transactivator |
T15843 |
494-497 |
DT |
denotes |
the |
T15842 |
491-493 |
IN |
denotes |
of |
T15841 |
480-490 |
NN |
denotes |
expression |
T15840 |
471-479 |
JJ |
denotes |
specific |
T15839 |
465-470 |
NN |
denotes |
liver |
T15838 |
462-464 |
IN |
denotes |
of |
T15837 |
455-461 |
NNS |
denotes |
levels |
T15836 |
450-454 |
JJ |
denotes |
high |
T15835 |
440-441 |
-RRB- |
denotes |
] |
T15834 |
438-440 |
CD |
denotes |
34 |
T15833 |
437-438 |
-LRB- |
denotes |
[ |
T15832 |
442-449 |
VBZ |
denotes |
directs |
T15831 |
422-423 |
HYPH |
denotes |
- |
T15830 |
423-426 |
NN |
denotes |
tTA |
T15829 |
419-422 |
NN |
denotes |
LAP |
T15828 |
427-436 |
NN |
denotes |
transgene |
T15827 |
415-418 |
DT |
denotes |
The |
T15826 |
414-659 |
sentence |
denotes |
The LAP-tTA transgene [34] directs high levels of liver specific expression of the tetracycline-controlled transactivator (tTA), a transcription factor that stimulates expression of promoters that harbor a transactivator response element (TRE). |
T15825 |
413-414 |
. |
denotes |
. |
T15824 |
412-413 |
-RRB- |
denotes |
) |
T15823 |
405-409 |
NN |
denotes |
Fig. |
T15822 |
410-412 |
NN |
denotes |
3A |
T15821 |
404-405 |
-LRB- |
denotes |
( |
T15820 |
390-391 |
HYPH |
denotes |
- |
T15819 |
391-393 |
NN |
denotes |
E2 |
T15818 |
387-390 |
NN |
denotes |
TRE |
T15817 |
394-403 |
NN |
denotes |
transgene |
T15816 |
385-386 |
DT |
denotes |
a |
T15815 |
381-384 |
CC |
denotes |
and |
T15814 |
366-367 |
HYPH |
denotes |
- |
T15813 |
367-370 |
NN |
denotes |
tTA |
T15812 |
363-366 |
NN |
denotes |
LAP |
T15811 |
371-380 |
NN |
denotes |
transgene |
T15810 |
361-362 |
DT |
denotes |
a |
T15809 |
358-360 |
IN |
denotes |
of |
T15808 |
348-357 |
VBD |
denotes |
consisted |
T15807 |
327-340 |
JJ |
denotes |
bi-transgenic |
T15806 |
341-347 |
NN |
denotes |
system |
T15805 |
322-326 |
DT |
denotes |
This |
T15804 |
321-414 |
sentence |
denotes |
This bi-transgenic system consisted of a LAP-tTA transgene and a TRE-E2 transgene (Fig. 3A). |
T15803 |
320-321 |
. |
denotes |
. |
T15802 |
301-309 |
NN |
denotes |
knockout |
T15801 |
298-300 |
NN |
denotes |
E2 |
T15800 |
310-320 |
NN |
denotes |
background |
T15799 |
294-297 |
DT |
denotes |
the |
T15798 |
291-293 |
IN |
denotes |
on |
T15797 |
285-290 |
NN |
denotes |
liver |
T15796 |
282-284 |
IN |
denotes |
in |
T15795 |
279-281 |
NN |
denotes |
E2 |
T15794 |
273-278 |
JJ |
denotes |
human |
T15793 |
265-272 |
VB |
denotes |
express |
T15792 |
262-264 |
TO |
denotes |
to |
T15791 |
244-254 |
JJ |
denotes |
transgenic |
T15790 |
239-243 |
NN |
denotes |
part |
T15789 |
235-238 |
CD |
denotes |
two |
T15788 |
255-261 |
NN |
denotes |
system |
T15787 |
233-234 |
DT |
denotes |
a |
T15786 |
230-232 |
IN |
denotes |
of |
T15785 |
217-220 |
VBD |
denotes |
was |
T15784 |
221-229 |
VBN |
denotes |
composed |
T15783 |
208-216 |
NN |
denotes |
strategy |
T15782 |
204-207 |
PRP$ |
denotes |
Our |
T15781 |
203-321 |
sentence |
denotes |
Our strategy was composed of a two part transgenic system to express human E2 in liver on the E2 knockout background. |
T15780 |
202-203 |
. |
denotes |
. |
T15779 |
191-196 |
NN |
denotes |
mouse |
T15778 |
186-190 |
NN |
denotes |
MSUD |
T15777 |
178-185 |
JJ |
denotes |
classic |
T15776 |
197-202 |
NN |
denotes |
model |
T15775 |
174-177 |
DT |
denotes |
the |
T15774 |
171-173 |
IN |
denotes |
in |
T15773 |
164-170 |
VBZ |
denotes |
occurs |
T15772 |
159-163 |
WDT |
denotes |
that |
T15771 |
149-158 |
NN |
denotes |
lethality |
T15770 |
140-148 |
JJ |
denotes |
neonatal |
T15769 |
136-139 |
CC |
denotes |
and |
T15768 |
131-135 |
NN |
denotes |
BCAA |
T15767 |
128-130 |
IN |
denotes |
of |
T15766 |
111-117 |
JJ |
denotes |
severe |
T15765 |
118-127 |
NN |
denotes |
elevation |
T15764 |
107-110 |
DT |
denotes |
the |
T15763 |
100-106 |
VB |
denotes |
rescue |
T15762 |
97-99 |
TO |
denotes |
to |
T15761 |
77-87 |
JJ |
denotes |
transgenic |
T15760 |
88-96 |
NN |
denotes |
strategy |
T15759 |
75-76 |
DT |
denotes |
a |
T15758 |
67-69 |
PRP |
denotes |
we |
T15757 |
65-67 |
, |
denotes |
, |
T15756 |
61-65 |
NN |
denotes |
MSUD |
T15755 |
58-60 |
IN |
denotes |
of |
T15754 |
45-52 |
NN |
denotes |
variant |
T15753 |
32-44 |
JJ |
denotes |
intermediate |
T15752 |
53-57 |
NN |
denotes |
form |
T15751 |
28-31 |
DT |
denotes |
the |
T15750 |
25-27 |
IN |
denotes |
of |
T15749 |
12-18 |
JJ |
denotes |
murine |
T15748 |
19-24 |
NN |
denotes |
model |
T15747 |
10-11 |
DT |
denotes |
a |
T15746 |
70-74 |
VBD |
denotes |
used |
T15745 |
3-9 |
VB |
denotes |
create |
T15744 |
0-2 |
TO |
denotes |
To |
T15894 |
783-784 |
. |
denotes |
. |
T15893 |
780-783 |
NN |
denotes |
tTA |
T15892 |
777-779 |
IN |
denotes |
by |
T15891 |
765-776 |
NN |
denotes |
stimulation |
T15890 |
760-764 |
IN |
denotes |
upon |
T15889 |
751-759 |
NN |
denotes |
promoter |
R3851 |
T15744 |
T15745 |
aux |
To,create |
R3852 |
T15745 |
T15746 |
advcl |
create,used |
R3853 |
T15747 |
T15748 |
det |
a,model |
R3854 |
T15748 |
T15745 |
dobj |
model,create |
R3855 |
T15749 |
T15748 |
amod |
murine,model |
R3856 |
T15750 |
T15748 |
prep |
of,model |
R3857 |
T15751 |
T15752 |
det |
the,form |
R3858 |
T15752 |
T15750 |
pobj |
form,of |
R3859 |
T15753 |
T15752 |
amod |
intermediate,form |
R3860 |
T15754 |
T15752 |
compound |
variant,form |
R3861 |
T15755 |
T15752 |
prep |
of,form |
R3862 |
T15756 |
T15755 |
pobj |
MSUD,of |
R3863 |
T15757 |
T15746 |
punct |
", ",used |
R3864 |
T15758 |
T15746 |
nsubj |
we,used |
R3865 |
T15759 |
T15760 |
det |
a,strategy |
R3866 |
T15760 |
T15746 |
dobj |
strategy,used |
R3867 |
T15761 |
T15760 |
amod |
transgenic,strategy |
R3868 |
T15762 |
T15763 |
aux |
to,rescue |
R3869 |
T15763 |
T15746 |
advcl |
rescue,used |
R3870 |
T15764 |
T15765 |
det |
the,elevation |
R3871 |
T15765 |
T15763 |
dobj |
elevation,rescue |
R3872 |
T15766 |
T15765 |
amod |
severe,elevation |
R3873 |
T15767 |
T15765 |
prep |
of,elevation |
R3874 |
T15768 |
T15767 |
pobj |
BCAA,of |
R3875 |
T15769 |
T15765 |
cc |
and,elevation |
R3876 |
T15770 |
T15771 |
amod |
neonatal,lethality |
R3877 |
T15771 |
T15765 |
conj |
lethality,elevation |
R3878 |
T15772 |
T15773 |
dep |
that,occurs |
R3879 |
T15773 |
T15771 |
relcl |
occurs,lethality |
R3880 |
T15774 |
T15773 |
prep |
in,occurs |
R3881 |
T15775 |
T15776 |
det |
the,model |
R3882 |
T15776 |
T15774 |
pobj |
model,in |
R3883 |
T15777 |
T15776 |
amod |
classic,model |
R3884 |
T15778 |
T15776 |
compound |
MSUD,model |
R3885 |
T15779 |
T15776 |
compound |
mouse,model |
R3886 |
T15780 |
T15746 |
punct |
.,used |
R3887 |
T15782 |
T15783 |
poss |
Our,strategy |
R3888 |
T15783 |
T15784 |
nsubjpass |
strategy,composed |
R3889 |
T15785 |
T15784 |
auxpass |
was,composed |
R3890 |
T15786 |
T15784 |
prep |
of,composed |
R3891 |
T15787 |
T15788 |
det |
a,system |
R3892 |
T15788 |
T15786 |
pobj |
system,of |
R3893 |
T15789 |
T15790 |
nummod |
two,part |
R3894 |
T15790 |
T15788 |
nmod |
part,system |
R3895 |
T15791 |
T15788 |
amod |
transgenic,system |
R3896 |
T15792 |
T15793 |
aux |
to,express |
R3897 |
T15793 |
T15788 |
advcl |
express,system |
R3898 |
T15794 |
T15795 |
amod |
human,E2 |
R3899 |
T15795 |
T15793 |
dobj |
E2,express |
R3900 |
T15796 |
T15793 |
prep |
in,express |
R3901 |
T15797 |
T15796 |
pobj |
liver,in |
R3902 |
T15798 |
T15793 |
prep |
on,express |
R3903 |
T15799 |
T15800 |
det |
the,background |
R3904 |
T15800 |
T15798 |
pobj |
background,on |
R3905 |
T15801 |
T15802 |
compound |
E2,knockout |
R3906 |
T15802 |
T15800 |
compound |
knockout,background |
R3907 |
T15803 |
T15784 |
punct |
.,composed |
R3908 |
T15805 |
T15806 |
det |
This,system |
R3909 |
T15806 |
T15808 |
nsubj |
system,consisted |
R3910 |
T15807 |
T15806 |
amod |
bi-transgenic,system |
R3911 |
T15809 |
T15808 |
prep |
of,consisted |
R3912 |
T15810 |
T15811 |
det |
a,transgene |
R3913 |
T15811 |
T15809 |
pobj |
transgene,of |
R3914 |
T15812 |
T15813 |
compound |
LAP,tTA |
R3915 |
T15813 |
T15811 |
compound |
tTA,transgene |
R3916 |
T15814 |
T15813 |
punct |
-,tTA |
R3917 |
T15815 |
T15811 |
cc |
and,transgene |
R3918 |
T15816 |
T15817 |
det |
a,transgene |
R3919 |
T15817 |
T15811 |
conj |
transgene,transgene |
R3920 |
T15818 |
T15819 |
compound |
TRE,E2 |
R3921 |
T15819 |
T15817 |
compound |
E2,transgene |
R3922 |
T15820 |
T15819 |
punct |
-,E2 |
R3923 |
T15821 |
T15822 |
punct |
(,3A |
R3924 |
T15822 |
T15808 |
parataxis |
3A,consisted |
R3925 |
T15823 |
T15822 |
compound |
Fig.,3A |
R3926 |
T15824 |
T15822 |
punct |
),3A |
R3927 |
T15825 |
T15808 |
punct |
.,consisted |
R3944 |
T15844 |
T15842 |
pobj |
transactivator,of |
R3945 |
T15845 |
T15846 |
npadvmod |
tetracycline,controlled |
R3946 |
T15846 |
T15844 |
amod |
controlled,transactivator |
R3947 |
T15847 |
T15846 |
punct |
-,controlled |
R3948 |
T15848 |
T15844 |
punct |
(,transactivator |
R3949 |
T15849 |
T15844 |
appos |
tTA,transactivator |
R3950 |
T15850 |
T15844 |
punct |
),transactivator |
R3951 |
T15851 |
T15844 |
punct |
", ",transactivator |
R3952 |
T15852 |
T15853 |
det |
a,factor |
R3953 |
T15853 |
T15844 |
appos |
factor,transactivator |
R3954 |
T15854 |
T15853 |
compound |
transcription,factor |
R3955 |
T15855 |
T15856 |
dep |
that,stimulates |
R3956 |
T15856 |
T15853 |
relcl |
stimulates,factor |
R3957 |
T15857 |
T15856 |
dobj |
expression,stimulates |
R3958 |
T15858 |
T15857 |
prep |
of,expression |
R3959 |
T15859 |
T15858 |
pobj |
promoters,of |
R3960 |
T15860 |
T15861 |
dep |
that,harbor |
R3961 |
T15861 |
T15859 |
relcl |
harbor,promoters |
R3962 |
T15862 |
T15863 |
det |
a,element |
R3963 |
T15863 |
T15861 |
dobj |
element,harbor |
R3964 |
T15864 |
T15863 |
compound |
transactivator,element |
R3965 |
T15865 |
T15863 |
compound |
response,element |
R3966 |
T15866 |
T15863 |
punct |
(,element |
R3967 |
T15867 |
T15863 |
appos |
TRE,element |
R3968 |
T15868 |
T15832 |
punct |
),directs |
R3969 |
T15869 |
T15832 |
punct |
.,directs |
R3970 |
T15871 |
T15872 |
det |
The,transgene |
R3971 |
T15872 |
T15876 |
nsubjpass |
transgene,designed |
R3972 |
T15873 |
T15874 |
compound |
TRE,E2 |
R3973 |
T15874 |
T15872 |
compound |
E2,transgene |
R3974 |
T15875 |
T15874 |
punct |
-,E2 |
R3975 |
T15877 |
T15876 |
auxpass |
was,designed |
R3976 |
T15878 |
T15879 |
aux |
to,express |
R3977 |
T15879 |
T15876 |
advcl |
express,designed |
R3978 |
T15880 |
T15881 |
det |
a,cDNA |
R3979 |
T15881 |
T15879 |
dobj |
cDNA,express |
R3980 |
T15882 |
T15881 |
amod |
human,cDNA |
R3981 |
T15883 |
T15881 |
compound |
E2,cDNA |
R3982 |
T15884 |
T15879 |
prep |
from,express |
R3983 |
T15885 |
T15886 |
det |
a,TRE |
R3984 |
T15886 |
T15884 |
pobj |
TRE,from |
R3985 |
T15887 |
T15886 |
acl |
containing,TRE |
R3986 |
T15888 |
T15889 |
amod |
minimal,promoter |
R3987 |
T15889 |
T15887 |
dobj |
promoter,containing |
R3988 |
T15890 |
T15879 |
prep |
upon,express |
R3989 |
T15891 |
T15890 |
pobj |
stimulation,upon |
R3990 |
T15892 |
T15891 |
prep |
by,stimulation |
R3991 |
T15893 |
T15892 |
pobj |
tTA,by |
R3992 |
T15894 |
T15876 |
punct |
.,designed |
R3928 |
T15827 |
T15828 |
det |
The,transgene |
R3929 |
T15828 |
T15832 |
nsubj |
transgene,directs |
R3930 |
T15829 |
T15830 |
compound |
LAP,tTA |
R3931 |
T15830 |
T15828 |
compound |
tTA,transgene |
R3932 |
T15831 |
T15830 |
punct |
-,tTA |
R3933 |
T15833 |
T15834 |
punct |
[,34 |
R3934 |
T15834 |
T15828 |
parataxis |
34,transgene |
R3935 |
T15835 |
T15834 |
punct |
],34 |
R3936 |
T15836 |
T15837 |
amod |
high,levels |
R3937 |
T15837 |
T15832 |
dobj |
levels,directs |
R3938 |
T15838 |
T15837 |
prep |
of,levels |
R3939 |
T15839 |
T15840 |
npadvmod |
liver,specific |
R3940 |
T15840 |
T15841 |
amod |
specific,expression |
R3941 |
T15841 |
T15838 |
pobj |
expression,of |
R3942 |
T15842 |
T15841 |
prep |
of,expression |
R3943 |
T15843 |
T15844 |
det |
the,transactivator |