> top > docs > PMC:1448208 > spans > 18852-22946 > annotations

PMC:1448208 / 18852-22946 JSONTXT

Annnotations TAB JSON ListView MergeView

craft-sa-dev

Id Subject Object Predicate Lexical cue
T12772 3986-3988 IN denotes to
T12771 3978-3985 JJ denotes similar
T12794 4093-4094 . denotes .
T12793 4089-4093 NN denotes MSUD
T12792 4081-4088 JJ denotes classic
T12791 4067-4074 RB denotes closely
T12790 4053-4061 NN denotes knockout
T12789 4062-4066 NNS denotes mice
T12788 4050-4052 NN denotes E2
T12787 4048-4050 , denotes ,
T12786 4075-4080 VBP denotes model
T12785 4044-4048 RB denotes Thus
T12784 4043-4094 sentence denotes Thus, E2 knockout mice closely model classic MSUD.
T12783 4042-4043 . denotes .
T12782 4038-4042 NN denotes MSUD
T12781 4035-4037 IN denotes of
T12780 4022-4029 JJ denotes classic
T12779 4030-4034 NN denotes form
T12778 4018-4021 DT denotes the
T12777 4013-4017 IN denotes with
T12776 4006-4012 NNS denotes humans
T12775 4003-4005 IN denotes in
T12774 3994-4002 VBN denotes observed
T12773 3989-3993 DT denotes that
T12770 3967-3977 RB denotes remarkably
T12769 3963-3966 VBP denotes are
T12768 3952-3962 NNS denotes phenotypes
T12767 3946-3951 DT denotes These
T12766 3945-4043 sentence denotes These phenotypes are remarkably similar to that observed in humans with the classic form of MSUD.
T12765 3944-3945 . denotes .
T12764 3935-3944 NN denotes lethality
T12763 3926-3934 JJ denotes neonatal
T12762 3923-3925 IN denotes in
T12761 3905-3915 RB denotes ultimately
T12760 3916-3922 VBP denotes result
T12759 3882-3891 JJ denotes metabolic
T12758 3892-3904 NNS denotes derangements
T12757 3876-3881 DT denotes These
T12756 3875-3945 sentence denotes These metabolic derangements ultimately result in neonatal lethality.
T12755 3874-3875 . denotes .
T12754 3869-3874 NN denotes urine
T12753 3865-3868 CC denotes and
T12752 3859-3864 NN denotes blood
T12751 3855-3858 DT denotes the
T12750 3852-3854 IN denotes in
T12749 3847-3851 NN denotes BCAA
T12748 3844-3846 IN denotes of
T12747 3837-3843 NNS denotes levels
T12746 3828-3836 VBN denotes elevated
T12745 3819-3827 RB denotes markedly
T12744 3814-3818 VBP denotes have
T12743 3810-3813 CC denotes and
T12742 3808-3810 , denotes ,
T12741 3792-3808 NN denotes immunoreactivity
T12740 3789-3791 NN denotes E2
T12739 3787-3789 , denotes ,
T12738 3769-3778 JJ denotes enzymatic
T12206 0-2 NN denotes E2
T12207 3-12 JJ denotes deficient
T12208 13-17 NNS denotes mice
T12209 29-34 VBP denotes model
T12210 18-28 RB denotes accurately
T12211 35-42 JJ denotes classic
T12212 43-47 NN denotes MSUD
T12213 47-241 sentence denotes To demonstrate that the gene targeting event created a true E2 null allele, we determined BCKDH activity in liver homogenates of postnatal day 1 mouse pups derived from +/- by +/- mating pairs.
T12214 48-50 TO denotes To
T12215 51-62 VB denotes demonstrate
T12216 127-137 VBD denotes determined
T12217 63-67 IN denotes that
T12218 93-100 VBD denotes created
T12219 68-71 DT denotes the
T12220 87-92 NN denotes event
T12221 72-76 NN denotes gene
T12222 77-86 NN denotes targeting
T12223 101-102 DT denotes a
T12224 116-122 NN denotes allele
T12225 103-107 JJ denotes true
T12226 108-110 NN denotes E2
T12227 111-115 JJ denotes null
T12228 122-124 , denotes ,
T12229 124-126 PRP denotes we
T12230 138-143 NN denotes BCKDH
T12231 144-152 NN denotes activity
T12232 153-155 IN denotes in
T12233 156-161 NN denotes liver
T12234 162-173 NNS denotes homogenates
T12235 174-176 IN denotes of
T12236 177-186 JJ denotes postnatal
T12237 187-190 NN denotes day
T12238 199-203 NNS denotes pups
T12239 191-192 CD denotes 1
T12247 221-223 IN denotes by
T12248 224-225 SYM denotes +
T12249 226-227 SYM denotes -
T12250 225-226 HYPH denotes /
T12251 228-234 NN denotes mating
T12252 240-241 . denotes .
T12253 241-319 sentence denotes As shown in Figure 2A, the BCKDH activity in +/+ mice was readily detectable.
T12254 242-244 IN denotes As
T12255 245-250 VBN denotes shown
T12256 296-299 VBD denotes was
T12257 251-253 IN denotes in
T12258 254-260 NN denotes Figure
T12259 261-263 NN denotes 2A
T12260 263-265 , denotes ,
T12261 265-268 DT denotes the
T12262 275-283 NN denotes activity
T12263 269-274 NN denotes BCKDH
T12264 284-286 IN denotes in
T12265 287-288 SYM denotes +
T12266 289-290 SYM denotes +
T12267 288-289 HYPH denotes /
T12268 291-295 NNS denotes mice
T12269 300-307 RB denotes readily
T12270 308-318 JJ denotes detectable
T12271 318-319 . denotes .
T12272 319-397 sentence denotes In marked contrast, BCKDH activity was completely absent in -/- mouse livers.
T12273 320-322 IN denotes In
T12274 355-358 VBD denotes was
T12275 323-329 JJ denotes marked
T12276 330-338 NN denotes contrast
T12277 338-340 , denotes ,
T12278 340-345 NN denotes BCKDH
T12279 346-354 NN denotes activity
T12280 359-369 RB denotes completely
T12281 370-376 JJ denotes absent
T12282 377-379 IN denotes in
T12283 380-381 SYM denotes -
T12284 382-383 SYM denotes -
T12285 381-382 HYPH denotes /
T12286 390-396 NNS denotes livers
T12287 384-389 NN denotes mouse
T12288 396-397 . denotes .
T12289 397-510 sentence denotes As expected, homogenates from +/- mice had approximately half the activity of their +/+ littermates (Figure 2A).
T12290 398-400 IN denotes As
T12291 401-409 VBN denotes expected
T12292 437-440 VBD denotes had
T12293 409-411 , denotes ,
T12294 411-422 NNS denotes homogenates
T12295 423-427 IN denotes from
T12296 428-429 SYM denotes +
T12297 430-431 SYM denotes -
T12298 429-430 HYPH denotes /
T12299 432-436 NNS denotes mice
T12300 441-454 RB denotes approximately
T12301 455-459 PDT denotes half
T12302 464-472 NN denotes activity
T12303 460-463 DT denotes the
T12304 473-475 IN denotes of
T12305 476-481 PRP$ denotes their
T12306 486-497 NNS denotes littermates
T12307 482-483 SYM denotes +
T12308 484-485 SYM denotes +
T12309 483-484 HYPH denotes /
T12310 498-499 -LRB- denotes (
T12311 506-508 NN denotes 2A
T12312 499-505 NN denotes Figure
T12313 508-509 -RRB- denotes )
T12314 509-510 . denotes .
T12315 510-598 sentence denotes The results from -/- mice are similar to that observed in humans with classic MSUD [1].
T12316 511-514 DT denotes The
T12317 515-522 NNS denotes results
T12318 537-540 VBP denotes are
T12319 523-527 IN denotes from
T12320 528-529 SYM denotes -
T12321 530-531 SYM denotes -
T12322 529-530 HYPH denotes /
T12323 532-536 NNS denotes mice
T12324 541-548 JJ denotes similar
T12325 549-551 IN denotes to
T12326 552-556 DT denotes that
T12327 557-565 VBN denotes observed
T12328 566-568 IN denotes in
T12329 569-575 NNS denotes humans
T12330 576-580 IN denotes with
T12331 581-588 JJ denotes classic
T12332 589-593 NN denotes MSUD
T12333 594-595 -LRB- denotes [
T12334 595-596 CD denotes 1
T12335 596-597 -RRB- denotes ]
T12336 597-598 . denotes .
T12337 598-599 sentence denotes
T12346 1487-1490 VBD denotes was
T12347 1496-1498 TO denotes to
T12348 1499-1506 VB denotes examine
T12349 1507-1509 NN denotes E2
T12350 1510-1517 NN denotes protein
T12351 1518-1520 IN denotes in
T12352 1521-1524 DT denotes the
T12353 1525-1529 NNS denotes mice
T12354 1529-1530 . denotes .
T12355 1530-1646 sentence denotes As shown in Figure 1C and 1D, immunoreactive E2 protein was abundant in liver and embryonic fibroblasts of+/+ mice.
T12356 1531-1533 IN denotes As
T12357 1534-1539 VBN denotes shown
T12358 1587-1590 VBD denotes was
T12359 1540-1542 IN denotes in
T12360 1543-1549 NN denotes Figure
T12361 1550-1552 NN denotes 1C
T12362 1553-1556 CC denotes and
T12363 1557-1559 NN denotes 1D
T12364 1559-1561 , denotes ,
T12365 1561-1575 JJ denotes immunoreactive
T12366 1579-1586 NN denotes protein
T12367 1576-1578 NN denotes E2
T12368 1591-1599 JJ denotes abundant
T12369 1600-1602 IN denotes in
T12370 1603-1608 NN denotes liver
T12371 1609-1612 CC denotes and
T12372 1613-1622 JJ denotes embryonic
T12373 1623-1634 NNS denotes fibroblasts
T12374 1635-1637 IN denotes of
T12375 1637-1638 SYM denotes +
T12376 1639-1640 SYM denotes +
T12377 1638-1639 HYPH denotes /
T12378 1641-1645 NNS denotes mice
T12379 1645-1646 . denotes .
T12380 1646-1738 sentence denotes In marked contrast, immunoreactive E2 protein was absent in these same tissues of -/- mice.
T12381 1647-1649 IN denotes In
T12382 1693-1696 VBD denotes was
T12383 1650-1656 JJ denotes marked
T12384 1657-1665 NN denotes contrast
T12385 1665-1667 , denotes ,
T12386 1667-1681 JJ denotes immunoreactive
T12387 1685-1692 NN denotes protein
T12388 1682-1684 NN denotes E2
T12389 1697-1703 JJ denotes absent
T12390 1704-1706 IN denotes in
T12391 1707-1712 DT denotes these
T12392 1718-1725 NNS denotes tissues
T12393 1713-1717 JJ denotes same
T12394 1726-1728 IN denotes of
T12395 1729-1730 SYM denotes -
T12396 1731-1732 SYM denotes -
T12397 1730-1731 HYPH denotes /
T12398 1733-1737 NNS denotes mice
T12399 1737-1738 . denotes .
T12400 1738-1937 sentence denotes Homozygous E2 knockout mice had a nearly 3-fold increase in blood (Figure 2B) and urine (data not shown) levels of BCAA (sum of leucine, isoleucine, and valine) as compared to their +/+ littermates.
T12401 1739-1749 JJ denotes Homozygous
T12402 1753-1761 NN denotes knockout
T12403 1750-1752 NN denotes E2
T12404 1762-1766 NNS denotes mice
T12405 1767-1770 VBD denotes had
T12406 1771-1772 DT denotes a
T12407 1787-1795 NN denotes increase
T12408 1773-1779 RB denotes nearly
T12409 1780-1781 CD denotes 3
T12410 1782-1786 JJ denotes fold
T12411 1781-1782 HYPH denotes -
T12412 1796-1798 IN denotes in
T12413 1799-1804 NN denotes blood
T12414 1844-1850 NNS denotes levels
T12415 1805-1806 -LRB- denotes (
T12416 1813-1815 NN denotes 2B
T12417 1806-1812 NN denotes Figure
T12418 1815-1816 -RRB- denotes )
T12419 1817-1820 CC denotes and
T12420 1821-1826 NN denotes urine
T12421 1827-1828 -LRB- denotes (
T12422 1837-1842 VBN denotes shown
T12423 1828-1832 NNS denotes data
T12424 1833-1836 RB denotes not
T12425 1842-1843 -RRB- denotes )
T12426 1851-1853 IN denotes of
T12427 1854-1858 NN denotes BCAA
T12428 1859-1860 -LRB- denotes (
T12429 1860-1863 NN denotes sum
T12430 1864-1866 IN denotes of
T12431 1867-1874 NN denotes leucine
T12432 1874-1876 , denotes ,
T12433 1876-1886 NN denotes isoleucine
T12434 1886-1888 , denotes ,
T12435 1888-1891 CC denotes and
T12436 1892-1898 NN denotes valine
T12437 1898-1899 -RRB- denotes )
T12438 1900-1902 IN denotes as
T12439 1903-1911 VBN denotes compared
T12440 1912-1914 IN denotes to
T12441 1915-1920 PRP$ denotes their
T12442 1925-1936 NNS denotes littermates
T12443 1921-1922 SYM denotes +
T12444 1923-1924 SYM denotes +
T12445 1922-1923 HYPH denotes /
T12446 1936-1937 . denotes .
T12447 1937-2113 sentence denotes Because amino acids were analyzed by tandem mass spectrometry, the sum of BCAA shown in Figure 2B also may include alloisoleucine that may have been produced in -/- MSUD mice.
T12448 1938-1945 IN denotes Because
T12449 1963-1971 VBN denotes analyzed
T12450 1946-1951 NN denotes amino
T12451 1952-1957 NNS denotes acids
T12452 1958-1962 VBD denotes were
T12453 2045-2052 VB denotes include
T12454 1972-1974 IN denotes by
T12455 1975-1981 NN denotes tandem
T12456 1987-1999 NN denotes spectrometry
T12457 1982-1986 NN denotes mass
T12458 1999-2001 , denotes ,
T12459 2001-2004 DT denotes the
T12460 2005-2008 NN denotes sum
T12461 2009-2011 IN denotes of
T12462 2012-2016 NN denotes BCAA
T12463 2017-2022 VBN denotes shown
T12464 2023-2025 IN denotes in
T12465 2026-2032 NN denotes Figure
T12466 2033-2035 NN denotes 2B
T12467 2036-2040 RB denotes also
T12468 2041-2044 MD denotes may
T12469 2053-2067 NN denotes alloisoleucine
T12470 2068-2072 WDT denotes that
T12471 2087-2095 VBN denotes produced
T12472 2073-2076 MD denotes may
T12473 2077-2081 VB denotes have
T12474 2082-2086 VBN denotes been
T12475 2096-2098 IN denotes in
T12476 2099-2100 SYM denotes -
T12477 2101-2102 SYM denotes -
T12478 2100-2101 HYPH denotes /
T12479 2108-2112 NNS denotes mice
T12480 2103-2107 NN denotes MSUD
T12481 2112-2113 . denotes .
T12482 2113-2208 sentence denotes The metabolism of BCAA is linked with the synthesis of alanine, glutamate, and glutamine [33].
T12483 2114-2117 DT denotes The
T12484 2118-2128 NN denotes metabolism
T12485 2140-2146 VBN denotes linked
T12486 2129-2131 IN denotes of
T12487 2132-2136 NN denotes BCAA
T12488 2137-2139 VBZ denotes is
T12489 2147-2151 IN denotes with
T12490 2152-2155 DT denotes the
T12491 2156-2165 NN denotes synthesis
T12492 2166-2168 IN denotes of
T12493 2169-2176 NN denotes alanine
T12494 2176-2178 , denotes ,
T12495 2178-2187 NN denotes glutamate
T12496 2187-2189 , denotes ,
T12497 2189-2192 CC denotes and
T12498 2193-2202 NN denotes glutamine
T12499 2203-2204 -LRB- denotes [
T12500 2204-2206 CD denotes 33
T12501 2206-2207 -RRB- denotes ]
T12502 2207-2208 . denotes .
T12503 2208-2401 sentence denotes Because of impaired metabolism of BCAA in MSUD mice, and to further characterize the abnormal biochemistry in this model, we analyzed the blood levels of the alanine, glutamate, and glutamine.
T12504 2209-2216 IN denotes Because
T12505 2334-2342 VBD denotes analyzed
T12506 2217-2219 IN denotes of
T12507 2220-2228 JJ denotes impaired
T12508 2229-2239 NN denotes metabolism
T12509 2240-2242 IN denotes of
T12510 2243-2247 NN denotes BCAA
T12511 2248-2250 IN denotes in
T12512 2251-2255 NN denotes MSUD
T12513 2256-2260 NNS denotes mice
T12514 2260-2262 , denotes ,
T12515 2262-2265 CC denotes and
T12516 2266-2268 TO denotes to
T12517 2277-2289 VB denotes characterize
T12518 2269-2276 RB denotes further
T12519 2290-2293 DT denotes the
T12520 2303-2315 NN denotes biochemistry
T12521 2294-2302 JJ denotes abnormal
T12522 2316-2318 IN denotes in
T12523 2319-2323 DT denotes this
T12524 2324-2329 NN denotes model
T12525 2329-2331 , denotes ,
T12526 2331-2333 PRP denotes we
T12527 2343-2346 DT denotes the
T12528 2353-2359 NNS denotes levels
T12529 2347-2352 NN denotes blood
T12530 2360-2362 IN denotes of
T12531 2363-2366 DT denotes the
T12532 2367-2374 NN denotes alanine
T12533 2374-2376 , denotes ,
T12534 2376-2385 NN denotes glutamate
T12535 2385-2387 , denotes ,
T12536 2387-2390 CC denotes and
T12537 2391-2400 NN denotes glutamine
T12538 2400-2401 . denotes .
T12539 2401-2533 sentence denotes As shown in Table 1, the levels of all three amino acids in homozygous mice were markedly lower than the levels in +/+ or +/- mice.
T12540 2402-2404 IN denotes As
T12541 2405-2410 VBN denotes shown
T12542 2478-2482 VBD denotes were
T12543 2411-2413 IN denotes in
T12544 2414-2419 NN denotes Table
T12545 2420-2421 CD denotes 1
T12546 2421-2423 , denotes ,
T12547 2423-2426 DT denotes the
T12548 2427-2433 NNS denotes levels
T12549 2434-2436 IN denotes of
T12550 2437-2440 DT denotes all
T12551 2453-2458 NNS denotes acids
T12552 2441-2446 CD denotes three
T12553 2447-2452 NN denotes amino
T12554 2459-2461 IN denotes in
T12555 2462-2472 JJ denotes homozygous
T12556 2473-2477 NNS denotes mice
T12557 2483-2491 RB denotes markedly
T12558 2492-2497 JJR denotes lower
T12559 2498-2502 IN denotes than
T12560 2503-2506 DT denotes the
T12561 2507-2513 NNS denotes levels
T12562 2514-2516 IN denotes in
T12563 2517-2518 SYM denotes +
T12564 2519-2520 SYM denotes +
T12565 2518-2519 HYPH denotes /
T12566 2528-2532 NNS denotes mice
T12567 2521-2523 CC denotes or
T12568 2524-2525 SYM denotes +
T12569 2526-2527 SYM denotes -
T12570 2525-2526 HYPH denotes /
T12571 2532-2533 . denotes .
T12572 2533-2629 sentence denotes The levels of these amino acids in the +/- mice were comparable to those in +/+ mice (Table 1).
T12573 2534-2537 DT denotes The
T12574 2538-2544 NNS denotes levels
T12575 2582-2586 VBD denotes were
T12576 2545-2547 IN denotes of
T12577 2548-2553 DT denotes these
T12578 2560-2565 NNS denotes acids
T12579 2554-2559 NN denotes amino
T12580 2566-2568 IN denotes in
T12581 2569-2572 DT denotes the
T12582 2577-2581 NNS denotes mice
T12583 2573-2574 SYM denotes +
T12584 2575-2576 SYM denotes -
T12585 2574-2575 HYPH denotes /
T12586 2587-2597 JJ denotes comparable
T12587 2598-2600 IN denotes to
T12588 2601-2606 DT denotes those
T12589 2607-2609 IN denotes in
T12590 2610-2611 SYM denotes +
T12591 2612-2613 SYM denotes +
T12592 2611-2612 HYPH denotes /
T12593 2614-2618 NNS denotes mice
T12594 2619-2620 -LRB- denotes (
T12595 2620-2625 NN denotes Table
T12596 2626-2627 CD denotes 1
T12597 2627-2628 -RRB- denotes )
T12598 2628-2629 . denotes .
T12599 2629-2935 sentence denotes Because of the abnormal decrease in the blood alanine level and marked rise in blood BCAA levels that are characteristic of MSUD, a recent report on MSUD patients has suggested that the ratio of BCAA/alanine provides a more sensitive measure of the abnormal biochemistry of MSUD than BCAA level alone [8].
T12600 2630-2637 IN denotes Because
T12601 2797-2806 VBN denotes suggested
T12602 2638-2640 IN denotes of
T12603 2641-2644 DT denotes the
T12604 2654-2662 NN denotes decrease
T12605 2645-2653 JJ denotes abnormal
T12606 2663-2665 IN denotes in
T12607 2666-2669 DT denotes the
T12608 2684-2689 NN denotes level
T12609 2670-2675 NN denotes blood
T12610 2676-2683 NN denotes alanine
T12611 2690-2693 CC denotes and
T12612 2694-2700 JJ denotes marked
T12613 2701-2705 NN denotes rise
T12614 2706-2708 IN denotes in
T12615 2709-2714 NN denotes blood
T12616 2720-2726 NNS denotes levels
T12617 2715-2719 NN denotes BCAA
T12618 2727-2731 WDT denotes that
T12619 2732-2735 VBP denotes are
T12620 2736-2750 JJ denotes characteristic
T12621 2751-2753 IN denotes of
T12622 2754-2758 NN denotes MSUD
T12623 2758-2760 , denotes ,
T12624 2760-2761 DT denotes a
T12625 2769-2775 NN denotes report
T12626 2762-2768 JJ denotes recent
T12627 2776-2778 IN denotes on
T12628 2779-2783 NN denotes MSUD
T12629 2784-2792 NNS denotes patients
T12630 2793-2796 VBZ denotes has
T12631 2807-2811 IN denotes that
T12632 2838-2846 VBZ denotes provides
T12633 2812-2815 DT denotes the
T12634 2816-2821 NN denotes ratio
T12635 2822-2824 IN denotes of
T12636 2825-2829 NN denotes BCAA
T12637 2830-2837 NN denotes alanine
T12638 2829-2830 HYPH denotes /
T12639 2847-2848 DT denotes a
T12640 2864-2871 NN denotes measure
T12641 2849-2853 RBR denotes more
T12642 2854-2863 JJ denotes sensitive
T12643 2872-2874 IN denotes of
T12644 2875-2878 DT denotes the
T12645 2888-2900 NN denotes biochemistry
T12646 2879-2887 JJ denotes abnormal
T12647 2901-2903 IN denotes of
T12648 2904-2908 NN denotes MSUD
T12649 2909-2913 IN denotes than
T12650 2914-2918 NN denotes BCAA
T12651 2919-2924 NN denotes level
T12652 2925-2930 RB denotes alone
T12653 2931-2932 -LRB- denotes [
T12654 2932-2933 CD denotes 8
T12655 2933-2934 -RRB- denotes ]
T12656 2934-2935 . denotes .
T12657 2935-3010 sentence denotes Therefore, we also expressed the amino acid results as BCAA/alanine ratio.
T12658 2936-2945 RB denotes Therefore
T12659 2955-2964 VBD denotes expressed
T12660 2945-2947 , denotes ,
T12661 2947-2949 PRP denotes we
T12662 2950-2954 RB denotes also
T12701 3099-3100 SYM denotes -
T12702 3098-3099 HYPH denotes /
T12703 3112-3113 . denotes .
T12704 3113-3220 sentence denotes These blood amino acid results are consistent with the concentrations seen in patients with MSUD [1,8,15].
T12705 3114-3119 DT denotes These
T12706 3137-3144 NNS denotes results
T12707 3120-3125 NN denotes blood
T12708 3126-3131 NN denotes amino
T12709 3132-3136 NN denotes acid
T12710 3145-3148 VBP denotes are
T12711 3149-3159 JJ denotes consistent
T12712 3160-3164 IN denotes with
T12713 3165-3168 DT denotes the
T12714 3169-3183 NNS denotes concentrations
T12715 3184-3188 VBN denotes seen
T12716 3189-3191 IN denotes in
T12717 3192-3200 NNS denotes patients
T12718 3201-3205 IN denotes with
T12719 3206-3210 NN denotes MSUD
T12720 3211-3212 -LRB- denotes [
T12721 3216-3218 CD denotes 15
T12722 3212-3213 CD denotes 1
T12723 3213-3214 , denotes ,
T12724 3214-3215 CD denotes 8
T12725 3215-3216 , denotes ,
T12726 3218-3219 -RRB- denotes ]
T12727 3219-3220 . denotes .
T22807 609-620 JJ denotes Biochemical
T22808 621-637 NN denotes characterization
T22809 638-640 IN denotes of
T22810 641-644 DT denotes the
T22811 665-670 NN denotes model
T22812 645-652 JJ denotes classic
T22813 653-657 NN denotes MSUD
T22814 658-664 JJ denotes murine
T22815 670-671 . denotes .
T22816 671-797 sentence denotes A, BCKDH enzyme activity in liver of newborn wild type control (+/+), heterozygous (+/-), and homozygous (-/-) knockout mice.
T22817 672-673 LS denotes A
T22818 688-696 NN denotes activity
T22819 673-675 , denotes ,
T22820 675-680 NN denotes BCKDH
T22821 681-687 NN denotes enzyme
T22822 697-699 IN denotes in
T22823 700-705 NN denotes liver
T22824 706-708 IN denotes of
T22825 709-716 JJ denotes newborn
T22826 792-796 NNS denotes mice
T22827 717-721 JJ denotes wild
T22828 722-726 NN denotes type
T22829 727-734 NN denotes control
T22830 783-791 NN denotes knockout
T22831 735-736 -LRB- denotes (
T22832 738-739 SYM denotes +
T22833 736-737 SYM denotes +
T22834 737-738 HYPH denotes /
T22835 739-740 -RRB- denotes )
T22836 740-742 , denotes ,
T22837 742-754 JJ denotes heterozygous
T22838 755-756 -LRB- denotes (
T22839 758-759 SYM denotes -
T22840 756-757 SYM denotes +
T22841 757-758 HYPH denotes /
T22842 759-760 -RRB- denotes )
T22843 760-762 , denotes ,
T22844 762-765 CC denotes and
T22845 766-776 JJ denotes homozygous
T22846 777-778 -LRB- denotes (
T22847 780-781 SYM denotes -
T22848 778-779 SYM denotes -
T22849 779-780 HYPH denotes /
T22850 781-782 -RRB- denotes )
T22851 796-797 . denotes .
T22852 797-920 sentence denotes Enzyme activity was significantly reduced in +/- liver compared to +/+, and was below the level of detection in -/- liver.
T22853 798-804 NN denotes Enzyme
T22854 805-813 NN denotes activity
T22855 814-817 VBD denotes was
T22856 818-831 RB denotes significantly
T22857 832-839 JJ denotes reduced
T22858 840-842 IN denotes in
T22859 843-844 SYM denotes +
T22860 845-846 SYM denotes -
T22861 844-845 HYPH denotes /
T22862 847-852 NN denotes liver
T22863 853-861 VBN denotes compared
T22864 862-864 IN denotes to
T22865 865-866 SYM denotes +
T22866 867-868 SYM denotes +
T22867 866-867 HYPH denotes /
T22868 868-870 , denotes ,
T22869 870-873 CC denotes and
T22870 874-877 VBD denotes was
T22871 878-883 IN denotes below
T22872 884-887 DT denotes the
T22873 888-893 NN denotes level
T22874 894-896 IN denotes of
T22875 897-906 NN denotes detection
T22876 907-909 IN denotes in
T22877 910-911 SYM denotes -
T22878 912-913 SYM denotes -
T22879 911-912 HYPH denotes /
T22880 914-919 NN denotes liver
T22881 919-920 . denotes .
T22882 920-967 sentence denotes B, Total BCAA concentrations in blood of mice.
T22883 921-922 LS denotes B
T22884 935-949 NNS denotes concentrations
T22885 922-924 , denotes ,
T22886 924-929 JJ denotes Total
T22887 930-934 NN denotes BCAA
T22888 950-952 IN denotes in
T22889 953-958 NN denotes blood
T22890 959-961 IN denotes of
T22891 962-966 NNS denotes mice
T22892 966-967 . denotes .
T22893 967-1032 sentence denotes Total BCAA represent the sum of leucine, isoleucine, and valine.
T22894 968-973 JJ denotes Total
T22895 974-978 NN denotes BCAA
T22896 979-988 VBP denotes represent
T22897 989-992 DT denotes the
T22898 993-996 NN denotes sum
T22899 997-999 IN denotes of
T22900 1000-1007 NN denotes leucine
T22901 1007-1009 , denotes ,
T22902 1009-1019 NN denotes isoleucine
T22903 1019-1021 , denotes ,
T22904 1021-1024 CC denotes and
T22905 1025-1031 NN denotes valine
T22906 1031-1032 . denotes .
T22907 1032-1134 sentence denotes Total BCAA concentrations in blood from -/- mice were significantly elevated compared to +/+ and +/-.
T22908 1033-1038 JJ denotes Total
T22909 1044-1058 NNS denotes concentrations
T22910 1039-1043 NN denotes BCAA
T22911 1082-1086 VBD denotes were
T22912 1059-1061 IN denotes in
T22913 1062-1067 NN denotes blood
T22914 1068-1072 IN denotes from
T22915 1073-1074 SYM denotes -
T22916 1075-1076 SYM denotes -
T22917 1074-1075 HYPH denotes /
T22918 1077-1081 NNS denotes mice
T22919 1087-1100 RB denotes significantly
T22920 1101-1109 JJ denotes elevated
T22921 1110-1118 VBN denotes compared
T22922 1119-1121 IN denotes to
T22923 1122-1123 SYM denotes +
T22924 1124-1125 SYM denotes +
T22925 1123-1124 HYPH denotes /
T22926 1126-1129 CC denotes and
T22927 1130-1131 SYM denotes +
T22928 1132-1133 SYM denotes -
T22929 1131-1132 HYPH denotes /
T22930 1133-1134 . denotes .
T22931 1134-1186 sentence denotes C, Ratio of total BCAA to alanine in blood of mice.
T22932 1135-1136 LS denotes C
T22933 1138-1143 NN denotes Ratio
T22934 1136-1138 , denotes ,
T22935 1144-1146 IN denotes of
T22936 1147-1152 JJ denotes total
T22937 1153-1157 NN denotes BCAA
T22938 1158-1160 IN denotes to
T22939 1161-1168 NN denotes alanine
T22940 1169-1171 IN denotes in
T22941 1172-1177 NN denotes blood
T22942 1178-1180 IN denotes of
T22943 1181-1185 NNS denotes mice
T22944 1185-1186 . denotes .
T22945 1186-1266 sentence denotes This ratio was significantly elevated in -/- mice compared to +/+ and +/- mice.
T22946 1187-1191 DT denotes This
T22947 1192-1197 NN denotes ratio
T22948 1198-1201 VBD denotes was
T22949 1202-1215 RB denotes significantly
T22950 1216-1224 JJ denotes elevated
T22951 1225-1227 IN denotes in
T22952 1228-1229 SYM denotes -
T22953 1230-1231 SYM denotes -
T22954 1229-1230 HYPH denotes /
T22955 1232-1236 NNS denotes mice
T22956 1237-1245 VBN denotes compared
T22957 1246-1248 IN denotes to
T22958 1249-1250 SYM denotes +
T22959 1251-1252 SYM denotes +
T22960 1250-1251 HYPH denotes /
T22961 1261-1265 NNS denotes mice
T22962 1253-1256 CC denotes and
T22963 1257-1258 SYM denotes +
T22964 1259-1260 SYM denotes -
T22965 1258-1259 HYPH denotes /
T22966 1265-1266 . denotes .
T22967 1266-1329 sentence denotes The numbers on the bars indicates the number of mice analyzed.
T22968 1267-1270 DT denotes The
T22969 1271-1278 NNS denotes numbers
T22970 1291-1300 VBZ denotes indicates
T22971 1279-1281 IN denotes on
T22972 1282-1285 DT denotes the
T22973 1286-1290 NNS denotes bars
T22974 1301-1304 DT denotes the
T22975 1305-1311 NN denotes number
T22976 1312-1314 IN denotes of
T22977 1315-1319 NNS denotes mice
T22978 1320-1328 VBN denotes analyzed
T22979 1328-1329 . denotes .
T22980 1329-1436 sentence denotes *, Significantly different from +/+ (P < 0.001); **, significantly different from +/+ and +/- (P < 0.001).
T22981 1330-1331 SYM denotes *
T22982 1379-1381 SYM denotes **
T22983 1331-1333 , denotes ,
T22984 1333-1346 RB denotes Significantly
T22985 1347-1356 JJ denotes different
T22986 1357-1361 IN denotes from
T22987 1362-1363 SYM denotes +
T22988 1364-1365 SYM denotes +
T22989 1363-1364 HYPH denotes /
T22990 1366-1367 -LRB- denotes (
T22991 1371-1376 CD denotes 0.001
T22992 1367-1368 NN denotes P
T22993 1369-1370 SYM denotes <
T22994 1376-1377 -RRB- denotes )
T22995 1377-1378 : denotes ;
T22996 1381-1383 , denotes ,
T22997 1383-1396 RB denotes significantly
T22998 1397-1406 JJ denotes different
T22999 1407-1411 IN denotes from
T23000 1412-1413 SYM denotes +
T23001 1414-1415 SYM denotes +
T23002 1413-1414 HYPH denotes /
T23003 1416-1419 CC denotes and
T23004 1420-1421 SYM denotes +
T23005 1422-1423 SYM denotes -
T23006 1421-1422 HYPH denotes /
T23007 1424-1425 -LRB- denotes (
T23008 1429-1434 CD denotes 0.001
T23009 1425-1426 NN denotes P
T23010 1427-1428 SYM denotes <
T23011 1434-1435 -RRB- denotes )
T23012 1435-1436 . denotes .
T24774 3230-3237 NN denotes Summary
T24775 3238-3240 IN denotes of
T24776 3241-3251 JJ denotes additional
T24777 3269-3275 NNS denotes levels
T24778 3252-3257 NN denotes blood
T24779 3258-3263 NN denotes amino
T24780 3264-3268 NN denotes acid
T24781 3276-3278 IN denotes in
T24782 3279-3282 DT denotes the
T24783 3296-3301 NN denotes model
T24784 3283-3290 JJ denotes classic
T24785 3291-3295 NN denotes MSUD
T24786 3302-3305 CC denotes and
T24787 3306-3313 NN denotes control
T24788 3314-3325 NNS denotes littermates
T24789 3326-3328 IN denotes as
T24790 3329-3339 VBN denotes determined
T24791 3340-3342 IN denotes by
T24792 3343-3349 JJ denotes tandem
T24793 3355-3367 NN denotes spectrometry
T24794 3350-3354 NN denotes mass
T24795 3367-3368 . denotes .
T24796 3368-3416 sentence denotes All samples were collected on the day of birth.
T24797 3369-3372 DT denotes All
T24798 3373-3380 NNS denotes samples
T24799 3386-3395 VBN denotes collected
T24800 3381-3385 VBD denotes were
T24801 3396-3398 IN denotes on
T24802 3399-3402 DT denotes the
T24803 3403-3406 NN denotes day
T24804 3407-3409 IN denotes of
T24805 3410-3415 NN denotes birth
T24806 3415-3416 . denotes .
T24807 3416-3445 sentence denotes All values are mean +/- SEM.
T24808 3417-3420 DT denotes All
T24809 3421-3427 NNS denotes values
T24810 3428-3431 VBP denotes are
T24811 3432-3436 NN denotes mean
T24812 3437-3438 SYM denotes +
T24813 3438-3439 HYPH denotes /
T24814 3439-3440 SYM denotes -
T24815 3441-3444 NN denotes SEM
T24816 3444-3445 . denotes .
T12690 3066-3070 IN denotes than
T12691 3073-3077 RB denotes fold
T12692 3072-3073 HYPH denotes -
T12693 3078-3084 JJR denotes higher
T12694 3085-3089 IN denotes than
T12695 3090-3091 SYM denotes +
T12696 3092-3093 SYM denotes +
T12697 3091-3092 HYPH denotes /
T12240 193-198 NN denotes mouse
T12241 204-211 VBN denotes derived
T12242 212-216 IN denotes from
T12243 217-218 SYM denotes +
T12244 219-220 SYM denotes -
T12245 218-219 HYPH denotes /
T12246 235-240 NNS denotes pairs
T12338 1437-1530 sentence denotes Immunohistochemistry with an E2 specific antibody was used to examine E2 protein in the mice.
T12339 1437-1457 NN denotes Immunohistochemistry
T12340 1491-1495 VBN denotes used
T12341 1458-1462 IN denotes with
T12342 1463-1465 DT denotes an
T12343 1478-1486 NN denotes antibody
T12344 1466-1468 NN denotes E2
T12345 1469-1477 JJ denotes specific
T12698 3101-3112 NNS denotes littermates
T12663 2965-2968 DT denotes the
T12664 2980-2987 NNS denotes results
T12665 2969-2974 NN denotes amino
T12666 2975-2979 NN denotes acid
T12667 2988-2990 IN denotes as
T12668 2991-2995 NN denotes BCAA
T12669 2996-3003 NN denotes alanine
T12670 2995-2996 HYPH denotes /
T12671 3004-3009 NN denotes ratio
T12672 3009-3010 . denotes .
T12673 3010-3113 sentence denotes As shown in Figure 2C, this ratio in -/- pups was more than 6-fold higher than +/+ or +/- littermates.
T12674 3011-3013 IN denotes As
T12675 3014-3019 VBN denotes shown
T12676 3057-3060 VBD denotes was
T12677 3020-3022 IN denotes in
T12678 3023-3029 NN denotes Figure
T12679 3030-3032 NN denotes 2C
T12680 3032-3034 , denotes ,
T12681 3034-3038 DT denotes this
T12682 3039-3044 NN denotes ratio
T12683 3045-3047 IN denotes in
T12684 3048-3049 SYM denotes -
T12685 3050-3051 SYM denotes -
T12686 3049-3050 HYPH denotes /
T12687 3052-3056 NNS denotes pups
T12688 3061-3065 RBR denotes more
T12689 3071-3072 CD denotes 6
T12699 3094-3096 CC denotes or
T12700 3097-3098 SYM denotes +
T12737 3779-3787 NN denotes activity
T12736 3763-3768 NN denotes BCKDH
T12735 3744-3752 NN denotes knockout
T12734 3753-3757 NNS denotes mice
T12733 3741-3743 NN denotes E2
T12732 3739-3741 , denotes ,
T12731 3732-3739 NN denotes summary
T12730 3758-3762 VBP denotes lack
T12729 3729-3731 IN denotes In
T12728 3220-3875 sentence denotes Table 1 Summary of additional blood amino acid levels in the classic MSUD model and control littermates as determined by tandem mass spectrometry. All samples were collected on the day of birth. All values are mean +/- SEM. MSUD Genotype Alanine Glutamate Glutamine (μmole/L) +/+ 196.1 ± 23.1 (6) 229.0 ± 12.0 (6) 76.8 ± 3.0 (6) +/- 167.6 ± 9.9 (13) 251.0 ± 12.8 (13) 72.3 ± 1.6 (13) -/- 94.6 ± 11.6* (5) 107.8 ± 4.8* (5) 50.4 ± 2.9* (5) *: Significantly different from +/+ and +/- (P < 0.001). In summary, E2 knockout mice lack BCKDH enzymatic activity, E2 immunoreactivity, and have markedly elevated levels of BCAA in the blood and urine.
R3290 T12206 T12207 npadvmod E2,deficient
R3291 T12207 T12208 amod deficient,mice
R3292 T12208 T12209 nsubj mice,model
R3293 T12210 T12209 advmod accurately,model
R3294 T12211 T12212 amod classic,MSUD
R3295 T12212 T12209 dobj MSUD,model
R3296 T12214 T12215 aux To,demonstrate
R3297 T12215 T12216 advcl demonstrate,determined
R3298 T12217 T12218 mark that,created
R3299 T12218 T12215 ccomp created,demonstrate
R3300 T12219 T12220 det the,event
R3301 T12220 T12218 nsubj event,created
R3302 T12221 T12222 compound gene,targeting
R3303 T12222 T12220 compound targeting,event
R3304 T12223 T12224 det a,allele
R3305 T12224 T12218 dobj allele,created
R3306 T12225 T12224 amod true,allele
R3307 T12226 T12227 npadvmod E2,null
R3308 T12227 T12224 amod null,allele
R3309 T12228 T12216 punct ", ",determined
R3310 T12229 T12216 nsubj we,determined
R3311 T12230 T12231 compound BCKDH,activity
R3312 T12231 T12216 dobj activity,determined
R3313 T12232 T12216 prep in,determined
R3314 T12233 T12234 compound liver,homogenates
R3315 T12234 T12232 pobj homogenates,in
R3316 T12235 T12234 prep of,homogenates
R3317 T12236 T12237 amod postnatal,day
R3318 T12237 T12238 nmod day,pups
R3319 T12238 T12235 pobj pups,of
R3320 T12239 T12237 nummod 1,day
R3321 T12240 T12238 compound mouse,pups
R3322 T12241 T12238 acl derived,pups
R3323 T12242 T12241 prep from,derived
R3324 T12243 T12244 punct +,-
R3325 T12244 T12246 punct -,pairs
R3326 T12245 T12244 punct /,-
R3327 T12246 T12242 pobj pairs,from
R3328 T12247 T12244 prep by,-
R3329 T12248 T12249 punct +,-
R3330 T12249 T12247 punct -,by
R3331 T12250 T12249 punct /,-
R3332 T12251 T12246 compound mating,pairs
R3333 T12252 T12216 punct .,determined
R3334 T12254 T12255 mark As,shown
R3335 T12255 T12256 advcl shown,was
R3336 T12257 T12255 prep in,shown
R3337 T12258 T12259 compound Figure,2A
R3338 T12259 T12257 pobj 2A,in
R3339 T12260 T12256 punct ", ",was
R3340 T12261 T12262 det the,activity
R3341 T12262 T12256 nsubj activity,was
R3342 T12263 T12262 compound BCKDH,activity
R3343 T12264 T12262 prep in,activity
R3344 T12265 T12266 punct +,+
R3346 T12267 T12266 punct /,+
R3363 T12286 T12282 pobj livers,in
R3364 T12287 T12286 compound mouse,livers
R3365 T12288 T12274 punct .,was
R3366 T12290 T12291 mark As,expected
R3367 T12291 T12292 advcl expected,had
R3368 T12293 T12292 punct ", ",had
R3369 T12294 T12292 nsubj homogenates,had
R3370 T12295 T12294 prep from,homogenates
R3371 T12296 T12297 punct +,-
R3372 T12297 T12299 punct -,mice
R3373 T12298 T12297 punct /,-
R3374 T12299 T12295 pobj mice,from
R3375 T12300 T12301 advmod approximately,half
R3376 T12301 T12302 nummod half,activity
R3377 T12302 T12292 dobj activity,had
R3378 T12303 T12302 det the,activity
R3379 T12304 T12302 prep of,activity
R3380 T12305 T12306 poss their,littermates
R3381 T12306 T12304 pobj littermates,of
R3382 T12307 T12308 punct +,+
R3383 T12308 T12306 punct +,littermates
R3384 T12309 T12308 punct /,+
R3385 T12310 T12311 punct (,2A
R3386 T12311 T12292 parataxis 2A,had
R3387 T12312 T12311 compound Figure,2A
R3388 T12313 T12311 punct ),2A
R3389 T12314 T12292 punct .,had
R3390 T12316 T12317 det The,results
R3391 T12317 T12318 nsubj results,are
R3392 T12319 T12317 prep from,results
R3393 T12320 T12321 punct -,-
R3394 T12321 T12323 punct -,mice
R3395 T12322 T12321 punct /,-
R3396 T12323 T12319 pobj mice,from
R3397 T12324 T12318 acomp similar,are
R3398 T12325 T12324 prep to,similar
R3399 T12326 T12325 pobj that,to
R3400 T12327 T12326 acl observed,that
R3401 T12328 T12327 prep in,observed
R3402 T12329 T12328 pobj humans,in
R3403 T12330 T12329 prep with,humans
R3404 T12331 T12332 amod classic,MSUD
R3405 T12332 T12330 pobj MSUD,with
R3406 T12333 T12334 punct [,1
R3407 T12334 T12318 parataxis 1,are
R3408 T12335 T12334 punct ],1
R3409 T12336 T12318 punct .,are
R3420 T12350 T12348 dobj protein,examine
R3421 T12351 T12348 prep in,examine
R3422 T12352 T12353 det the,mice
R3423 T12353 T12351 pobj mice,in
R3424 T12354 T12340 punct .,used
R3425 T12356 T12357 mark As,shown
R3426 T12357 T12358 advcl shown,was
R3427 T12359 T12357 prep in,shown
R3428 T12360 T12361 compound Figure,1C
R3429 T12361 T12359 pobj 1C,in
R3430 T12362 T12361 cc and,1C
R3431 T12363 T12361 conj 1D,1C
R3432 T12364 T12358 punct ", ",was
R3433 T12365 T12366 amod immunoreactive,protein
R3434 T12366 T12358 nsubj protein,was
R3435 T12367 T12366 compound E2,protein
R3436 T12368 T12358 acomp abundant,was
R3437 T12369 T12358 prep in,was
R3438 T12370 T12369 pobj liver,in
R3439 T12371 T12370 cc and,liver
R3440 T12372 T12373 amod embryonic,fibroblasts
R3441 T12373 T12370 conj fibroblasts,liver
R3442 T12374 T12370 prep of,liver
R3443 T12375 T12376 punct +,+
R3444 T12376 T12378 punct +,mice
R3445 T12377 T12376 punct /,+
R3446 T12378 T12374 pobj mice,of
R3447 T12379 T12358 punct .,was
R3448 T12381 T12382 prep In,was
R3449 T12383 T12384 amod marked,contrast
R3450 T12384 T12381 pobj contrast,In
R3451 T12385 T12382 punct ", ",was
R3452 T12386 T12387 amod immunoreactive,protein
R3453 T12387 T12382 nsubj protein,was
R3454 T12388 T12387 compound E2,protein
R3455 T12389 T12382 acomp absent,was
R3456 T12390 T12382 prep in,was
R3457 T12391 T12392 det these,tissues
R3458 T12392 T12390 pobj tissues,in
R3459 T12393 T12392 amod same,tissues
R3460 T12394 T12392 prep of,tissues
R3461 T12395 T12396 punct -,-
R3462 T12396 T12398 punct -,mice
R3463 T12397 T12396 punct /,-
R3464 T12398 T12394 pobj mice,of
R3465 T12399 T12382 punct .,was
R3466 T12401 T12402 amod Homozygous,knockout
R3467 T12402 T12404 compound knockout,mice
R3468 T12403 T12402 compound E2,knockout
R3469 T12404 T12405 nsubj mice,had
R3470 T12406 T12407 det a,increase
R3471 T12407 T12405 dobj increase,had
R3472 T12408 T12409 advmod nearly,3
R3473 T12409 T12410 quantmod 3,fold
R3474 T12410 T12407 nummod fold,increase
R3475 T12411 T12410 punct -,fold
R3476 T12412 T12407 prep in,increase
R3477 T12413 T12414 nmod blood,levels
R3478 T12414 T12412 pobj levels,in
R3479 T12415 T12416 punct (,2B
R3480 T12416 T12413 parataxis 2B,blood
R3481 T12417 T12416 compound Figure,2B
R3482 T12418 T12416 punct ),2B
R3483 T12419 T12413 cc and,blood
R3484 T12420 T12413 conj urine,blood
R3485 T12421 T12422 punct (,shown
R3486 T12422 T12420 parataxis shown,urine
R3487 T12423 T12422 nsubj data,shown
R3488 T12424 T12422 neg not,shown
R3489 T12425 T12422 punct ),shown
R3490 T12426 T12414 prep of,levels
R3491 T12427 T12426 pobj BCAA,of
R3492 T12428 T12429 punct (,sum
R3493 T12429 T12427 parataxis sum,BCAA
R3494 T12430 T12429 prep of,sum
R3495 T12431 T12430 pobj leucine,of
R3496 T12432 T12431 punct ", ",leucine
R3497 T12433 T12431 conj isoleucine,leucine
R3498 T12434 T12433 punct ", ",isoleucine
R3499 T12435 T12433 cc and,isoleucine
R3500 T12436 T12433 conj valine,isoleucine
R3501 T12437 T12429 punct ),sum
R3502 T12438 T12407 prep as,increase
R3503 T12439 T12438 prep compared,as
R3504 T12440 T12439 prep to,compared
R3505 T12441 T12442 poss their,littermates
R3506 T12442 T12440 pobj littermates,to
R3507 T12443 T12444 punct +,+
R3508 T12444 T12442 punct +,littermates
R3509 T12445 T12444 punct /,+
R3510 T12446 T12405 punct .,had
R3511 T12448 T12449 mark Because,analyzed
R3512 T12449 T12453 advcl analyzed,include
R3513 T12450 T12451 compound amino,acids
R3514 T12451 T12449 nsubjpass acids,analyzed
R3515 T12452 T12449 auxpass were,analyzed
R3516 T12454 T12449 prep by,analyzed
R3517 T12455 T12456 compound tandem,spectrometry
R3518 T12456 T12454 pobj spectrometry,by
R3519 T12457 T12456 compound mass,spectrometry
R3520 T12458 T12453 punct ", ",include
R3521 T12459 T12460 det the,sum
R3522 T12460 T12453 nsubj sum,include
R3523 T12461 T12460 prep of,sum
R3524 T12462 T12461 pobj BCAA,of
R3525 T12463 T12460 acl shown,sum
R3526 T12464 T12463 prep in,shown
R3527 T12465 T12466 compound Figure,2B
R3528 T12466 T12464 pobj 2B,in
R3529 T12467 T12453 advmod also,include
R3530 T12468 T12453 aux may,include
R3531 T12469 T12453 dobj alloisoleucine,include
R3532 T12470 T12471 dep that,produced
R3533 T12471 T12469 relcl produced,alloisoleucine
R3534 T12472 T12471 aux may,produced
R3535 T12473 T12471 aux have,produced
R3536 T12474 T12471 auxpass been,produced
R3537 T12475 T12471 prep in,produced
R3538 T12476 T12477 punct -,-
R3539 T12477 T12479 punct -,mice
R3540 T12478 T12477 punct /,-
R3541 T12479 T12475 pobj mice,in
R3542 T12480 T12479 compound MSUD,mice
R3543 T12481 T12453 punct .,include
R3544 T12483 T12484 det The,metabolism
R3545 T12484 T12485 nsubjpass metabolism,linked
R3546 T12486 T12484 prep of,metabolism
R3547 T12487 T12486 pobj BCAA,of
R3548 T12488 T12485 auxpass is,linked
R3549 T12489 T12485 prep with,linked
R3550 T12490 T12491 det the,synthesis
R3551 T12491 T12489 pobj synthesis,with
R3552 T12492 T12491 prep of,synthesis
R3553 T12493 T12492 pobj alanine,of
R3554 T12494 T12493 punct ", ",alanine
R3555 T12495 T12493 conj glutamate,alanine
R3556 T12496 T12495 punct ", ",glutamate
R3557 T12497 T12495 cc and,glutamate
R3558 T12498 T12495 conj glutamine,glutamate
R3559 T12499 T12500 punct [,33
R3560 T12500 T12485 parataxis 33,linked
R3561 T12501 T12500 punct ],33
R3562 T12502 T12485 punct .,linked
R3563 T12504 T12505 prep Because,analyzed
R3564 T12506 T12504 pcomp of,Because
R3565 T12507 T12508 amod impaired,metabolism
R3566 T12508 T12504 pobj metabolism,Because
R3567 T12509 T12508 prep of,metabolism
R3568 T12510 T12509 pobj BCAA,of
R3569 T12511 T12508 prep in,metabolism
R3570 T12512 T12513 compound MSUD,mice
R3571 T12513 T12511 pobj mice,in
R3572 T12514 T12504 punct ", ",Because
R3573 T12515 T12504 cc and,Because
R3574 T12516 T12517 aux to,characterize
R3575 T12517 T12504 conj characterize,Because
R3576 T12518 T12517 advmod further,characterize
R3577 T12519 T12520 det the,biochemistry
R3578 T12520 T12517 dobj biochemistry,characterize
R3579 T12521 T12520 amod abnormal,biochemistry
R3580 T12522 T12517 prep in,characterize
R3581 T12523 T12524 det this,model
R3582 T12524 T12522 pobj model,in
R3583 T12525 T12505 punct ", ",analyzed
R3584 T12526 T12505 nsubj we,analyzed
R3585 T12527 T12528 det the,levels
R3586 T12528 T12505 dobj levels,analyzed
R3587 T12529 T12528 compound blood,levels
R3588 T12530 T12528 prep of,levels
R3589 T12531 T12532 det the,alanine
R3590 T12532 T12530 pobj alanine,of
R3591 T12533 T12532 punct ", ",alanine
R3592 T12534 T12532 conj glutamate,alanine
R3593 T12535 T12534 punct ", ",glutamate
R3594 T12536 T12534 cc and,glutamate
R3595 T12537 T12534 conj glutamine,glutamate
R3596 T12538 T12505 punct .,analyzed
R3597 T12540 T12541 mark As,shown
R3598 T12541 T12542 advcl shown,were
R3599 T12543 T12541 prep in,shown
R3600 T12544 T12543 pobj Table,in
R3601 T12545 T12544 nummod 1,Table
R3602 T12546 T12542 punct ", ",were
R3603 T12547 T12548 det the,levels
R3604 T12548 T12542 nsubj levels,were
R3605 T12549 T12548 prep of,levels
R3606 T12550 T12551 det all,acids
R3607 T12551 T12549 pobj acids,of
R3608 T12552 T12551 nummod three,acids
R3609 T12553 T12551 compound amino,acids
R3610 T12554 T12548 prep in,levels
R3611 T12555 T12556 amod homozygous,mice
R3612 T12556 T12554 pobj mice,in
R3613 T12557 T12558 advmod markedly,lower
R3614 T12558 T12542 acomp lower,were
R3615 T12559 T12558 prep than,lower
R3616 T12560 T12561 det the,levels
R3617 T12561 T12559 pobj levels,than
R3618 T12562 T12561 prep in,levels
R3619 T12563 T12564 punct +,+
R3620 T12564 T12566 punct +,mice
R3621 T12565 T12564 punct /,+
R3622 T12566 T12562 pobj mice,in
R3623 T12567 T12564 cc or,+
R3624 T12568 T12569 punct +,-
R3625 T12569 T12564 conj -,+
R3626 T12570 T12569 punct /,-
R3627 T12571 T12542 punct .,were
R3628 T12573 T12574 det The,levels
R3629 T12574 T12575 nsubj levels,were
R3630 T12576 T12574 prep of,levels
R3631 T12577 T12578 det these,acids
R3632 T12578 T12576 pobj acids,of
R3633 T12579 T12578 compound amino,acids
R3634 T12580 T12574 prep in,levels
R3635 T12581 T12582 det the,mice
R3636 T12582 T12580 pobj mice,in
R3637 T12583 T12584 punct +,-
R3638 T12584 T12582 punct -,mice
R3639 T12585 T12584 punct /,-
R3640 T12586 T12575 acomp comparable,were
R3641 T12587 T12586 prep to,comparable
R3642 T12588 T12587 pobj those,to
R3643 T12589 T12588 prep in,those
R3644 T12590 T12591 punct +,+
R3645 T12591 T12593 punct +,mice
R3646 T12592 T12591 punct /,+
R3647 T12593 T12589 pobj mice,in
R3648 T12594 T12595 punct (,Table
R3649 T12595 T12575 parataxis Table,were
R3650 T12596 T12595 nummod 1,Table
R3651 T12597 T12595 punct ),Table
R3652 T12598 T12575 punct .,were
R3653 T12600 T12601 prep Because,suggested
R3654 T12602 T12600 pcomp of,Because
R3655 T12603 T12604 det the,decrease
R3656 T12604 T12600 pobj decrease,Because
R3657 T12605 T12604 amod abnormal,decrease
R3658 T12606 T12604 prep in,decrease
R3659 T12607 T12608 det the,level
R3660 T12608 T12606 pobj level,in
R3661 T12609 T12608 compound blood,level
R3662 T12610 T12608 compound alanine,level
R3663 T12611 T12604 cc and,decrease
R3664 T12612 T12613 amod marked,rise
R3665 T12613 T12604 conj rise,decrease
R3666 T12614 T12613 prep in,rise
R3667 T12615 T12616 compound blood,levels
R3668 T12616 T12614 pobj levels,in
R3669 T12617 T12616 compound BCAA,levels
R3670 T12618 T12619 dep that,are
R3671 T12619 T12604 relcl are,decrease
R3672 T12620 T12619 acomp characteristic,are
R3673 T12621 T12620 prep of,characteristic
R3674 T12622 T12621 pobj MSUD,of
R3675 T12623 T12601 punct ", ",suggested
R3676 T12624 T12625 det a,report
R3677 T12625 T12601 nsubj report,suggested
R3678 T12626 T12625 amod recent,report
R3679 T12627 T12625 prep on,report
R3680 T12628 T12629 compound MSUD,patients
R3681 T12629 T12627 pobj patients,on
R3682 T12630 T12601 aux has,suggested
R3683 T12631 T12632 mark that,provides
R3684 T12632 T12601 ccomp provides,suggested
R3685 T12633 T12634 det the,ratio
R3686 T12634 T12632 nsubj ratio,provides
R3687 T12635 T12634 prep of,ratio
R3688 T12636 T12637 compound BCAA,alanine
R3689 T12637 T12635 pobj alanine,of
R3690 T12638 T12637 punct /,alanine
R3691 T12639 T12640 det a,measure
R3692 T12640 T12632 dobj measure,provides
R3693 T12641 T12642 advmod more,sensitive
R3694 T12642 T12640 amod sensitive,measure
R3695 T12643 T12640 prep of,measure
R3696 T12644 T12645 det the,biochemistry
R3697 T12645 T12643 pobj biochemistry,of
R3698 T12646 T12645 amod abnormal,biochemistry
R3699 T12647 T12645 prep of,biochemistry
R3700 T12648 T12647 pobj MSUD,of
R3701 T12649 T12640 prep than,measure
R3702 T12650 T12651 compound BCAA,level
R3703 T12651 T12649 pobj level,than
R3704 T12652 T12651 advmod alone,level
R3705 T12653 T12654 punct [,8
R3706 T12654 T12601 parataxis 8,suggested
R3707 T12655 T12654 punct ],8
R3708 T12656 T12601 punct .,suggested
R3709 T12658 T12659 advmod Therefore,expressed
R3710 T12660 T12659 punct ", ",expressed
R3711 T12661 T12659 nsubj we,expressed
R3712 T12662 T12659 advmod also,expressed
R3713 T12663 T12664 det the,results
R3714 T12664 T12659 dobj results,expressed
R3715 T12665 T12666 compound amino,acid
R3716 T12666 T12664 compound acid,results
R3717 T12667 T12659 prep as,expressed
R3718 T12668 T12669 compound BCAA,alanine
R3719 T12669 T12671 compound alanine,ratio
R3720 T12670 T12669 punct /,alanine
R3748 T12700 T12701 punct +,-
R3749 T12701 T12696 conj -,+
R3750 T12702 T12701 punct /,-
R3751 T12703 T12676 punct .,was
R3752 T12705 T12706 det These,results
R3753 T12706 T12710 nsubj results,are
R3754 T12707 T12706 compound blood,results
R3755 T12708 T12709 compound amino,acid
R3756 T12709 T12706 compound acid,results
R3757 T12711 T12710 acomp consistent,are
R3758 T12712 T12711 prep with,consistent
R3759 T12713 T12714 det the,concentrations
R3760 T12714 T12712 pobj concentrations,with
R3761 T12715 T12714 acl seen,concentrations
R3762 T12716 T12715 prep in,seen
R3763 T12717 T12716 pobj patients,in
R3764 T12718 T12717 prep with,patients
R3765 T12719 T12718 pobj MSUD,with
R3766 T12720 T12721 punct [,15
R3767 T12721 T12710 parataxis 15,are
R3768 T12722 T12721 nummod 1,15
R3769 T12723 T12721 punct ",",15
R3770 T12724 T12721 nummod 8,15
R3771 T12725 T12721 punct ",",15
R3772 T12726 T12721 punct ],15
R3773 T12727 T12710 punct .,are
R3803 T12761 T12760 advmod ultimately,result
R3804 T12762 T12760 prep in,result
R3805 T12763 T12764 amod neonatal,lethality
R3806 T12764 T12762 pobj lethality,in
R3807 T12765 T12760 punct .,result
R3808 T12767 T12768 det These,phenotypes
R3809 T12768 T12769 nsubj phenotypes,are
R3810 T12770 T12771 advmod remarkably,similar
R3811 T12771 T12769 acomp similar,are
R3812 T12772 T12771 prep to,similar
R3813 T12773 T12772 pobj that,to
R3814 T12774 T12773 acl observed,that
R3815 T12775 T12774 prep in,observed
R3816 T12776 T12775 pobj humans,in
R3817 T12777 T12776 prep with,humans
R3818 T12778 T12779 det the,form
R3819 T12779 T12777 pobj form,with
R3820 T12780 T12779 amod classic,form
R3821 T12781 T12779 prep of,form
R3822 T12782 T12781 pobj MSUD,of
R3823 T12783 T12769 punct .,are
R3824 T12785 T12786 advmod Thus,model
R3825 T12787 T12786 punct ", ",model
R3826 T12788 T12789 compound E2,mice
R3827 T12789 T12786 nsubj mice,model
R3828 T12790 T12789 compound knockout,mice
R3829 T12791 T12786 advmod closely,model
R3830 T12792 T12793 amod classic,MSUD
R3831 T12793 T12786 dobj MSUD,model
R3832 T12794 T12786 punct .,model
R6552 T22807 T22808 amod Biochemical,characterization
R6553 T22809 T22808 prep of,characterization
R6554 T22810 T22811 det the,model
R6555 T22811 T22809 pobj model,of
R6556 T22812 T22811 amod classic,model
R6557 T22813 T22811 nmod MSUD,model
R6558 T22814 T22811 amod murine,model
R6559 T22815 T22808 punct .,characterization
R6560 T22817 T22818 meta A,activity
R6561 T22819 T22818 punct ", ",activity
R6562 T22820 T22818 compound BCKDH,activity
R6563 T22821 T22818 compound enzyme,activity
R6564 T22822 T22818 prep in,activity
R6565 T22823 T22822 pobj liver,in
R6566 T22824 T22823 prep of,liver
R6567 T22825 T22826 amod newborn,mice
R6568 T22826 T22824 pobj mice,of
R6569 T22827 T22828 amod wild,type
R6570 T22828 T22829 nmod type,control
R6571 T22829 T22830 nmod control,knockout
R6572 T22830 T22826 compound knockout,mice
R6573 T22831 T22832 punct (,+
R6574 T22832 T22829 punct +,control
R6575 T22833 T22832 punct +,+
R6576 T22834 T22832 punct /,+
R6577 T22835 T22832 punct ),+
R6578 T22836 T22829 punct ", ",control
R6579 T22837 T22829 conj heterozygous,control
R6580 T22838 T22839 punct (,-
R6581 T22839 T22837 punct -,heterozygous
R6582 T22840 T22839 punct +,-
R6583 T22841 T22839 punct /,-
R6584 T22842 T22839 punct ),-
R6585 T22843 T22837 punct ", ",heterozygous
R6586 T22844 T22837 cc and,heterozygous
R6587 T22845 T22837 conj homozygous,heterozygous
R6588 T22846 T22847 punct (,-
R6589 T22847 T22845 punct -,homozygous
R6590 T22848 T22847 punct -,-
R6591 T22849 T22847 punct /,-
R6592 T22850 T22847 punct ),-
R6593 T22851 T22818 punct .,activity
R6594 T22853 T22854 compound Enzyme,activity
R6595 T22854 T22855 nsubj activity,was
R6596 T22856 T22857 advmod significantly,reduced
R6597 T22857 T22855 acomp reduced,was
R6598 T22858 T22855 prep in,was
R6599 T22859 T22860 punct +,-
R6600 T22860 T22862 punct -,liver
R6601 T22861 T22860 punct /,-
R6602 T22862 T22858 pobj liver,in
R6603 T22863 T22855 prep compared,was
R6604 T22864 T22863 prep to,compared
R6605 T22865 T22866 punct +,+
R6606 T22866 T22864 punct +,to
R6607 T22867 T22866 punct /,+
R6608 T22868 T22855 punct ", ",was
R6609 T22869 T22855 cc and,was
R6610 T22870 T22855 conj was,was
R6611 T22871 T22870 prep below,was
R6612 T22872 T22873 det the,level
R6613 T22873 T22871 pobj level,below
R6614 T22874 T22873 prep of,level
R6615 T22875 T22874 pobj detection,of
R6616 T22876 T22870 prep in,was
R6617 T22877 T22878 punct -,-
R6618 T22878 T22880 punct -,liver
R6619 T22879 T22878 punct /,-
R6620 T22880 T22876 pobj liver,in
R6621 T22881 T22855 punct .,was
R6622 T22883 T22884 meta B,concentrations
R6623 T22885 T22884 punct ", ",concentrations
R6624 T22886 T22884 amod Total,concentrations
R6625 T22887 T22884 compound BCAA,concentrations
R6626 T22888 T22884 prep in,concentrations
R6627 T22889 T22888 pobj blood,in
R6628 T22890 T22889 prep of,blood
R6629 T22891 T22890 pobj mice,of
R6630 T22892 T22884 punct .,concentrations
R6631 T22894 T22895 amod Total,BCAA
R6632 T22895 T22896 nsubj BCAA,represent
R6633 T22897 T22898 det the,sum
R6634 T22898 T22896 dobj sum,represent
R6635 T22899 T22898 prep of,sum
R6636 T22900 T22899 pobj leucine,of
R6637 T22901 T22900 punct ", ",leucine
R6638 T22902 T22900 conj isoleucine,leucine
R6639 T22903 T22902 punct ", ",isoleucine
R6640 T22904 T22902 cc and,isoleucine
R6641 T22905 T22902 conj valine,isoleucine
R6642 T22906 T22896 punct .,represent
R6643 T22908 T22909 amod Total,concentrations
R6644 T22909 T22911 nsubj concentrations,were
R6645 T22910 T22909 compound BCAA,concentrations
R6646 T22912 T22909 prep in,concentrations
R6647 T22913 T22912 pobj blood,in
R6648 T22914 T22913 prep from,blood
R6649 T22915 T22916 punct -,-
R6650 T22916 T22918 punct -,mice
R6651 T22917 T22916 punct /,-
R6652 T22918 T22914 pobj mice,from
R6653 T22919 T22920 advmod significantly,elevated
R6654 T22920 T22911 acomp elevated,were
R6655 T22921 T22911 prep compared,were
R6656 T22922 T22921 prep to,compared
R6657 T22923 T22924 punct +,+
R6658 T22924 T22922 punct +,to
R6659 T22925 T22924 punct /,+
R6660 T22926 T22924 cc and,+
R6661 T22927 T22928 punct +,-
R6662 T22928 T22924 conj -,+
R6663 T22929 T22928 punct /,-
R6664 T22930 T22911 punct .,were
R6665 T22932 T22933 meta C,Ratio
R6666 T22934 T22933 punct ", ",Ratio
R6667 T22935 T22933 prep of,Ratio
R6668 T22936 T22937 amod total,BCAA
R6669 T22937 T22935 pobj BCAA,of
R6670 T22938 T22933 prep to,Ratio
R6671 T22939 T22938 pobj alanine,to
R6672 T22940 T22933 prep in,Ratio
R6673 T22941 T22940 pobj blood,in
R6674 T22942 T22941 prep of,blood
R6675 T22943 T22942 pobj mice,of
R6676 T22944 T22933 punct .,Ratio
R6677 T22946 T22947 det This,ratio
R6678 T22947 T22948 nsubj ratio,was
R6679 T22949 T22950 advmod significantly,elevated
R6680 T22950 T22948 acomp elevated,was
R6681 T22951 T22948 prep in,was
R6682 T22952 T22953 punct -,-
R6683 T22953 T22955 punct -,mice
R6684 T22954 T22953 punct /,-
R6685 T22955 T22951 pobj mice,in
R6686 T22956 T22948 prep compared,was
R6687 T22957 T22956 prep to,compared
R6688 T22958 T22959 punct +,+
R6689 T22959 T22961 punct +,mice
R6690 T22960 T22959 punct /,+
R6691 T22961 T22957 pobj mice,to
R6692 T22962 T22959 cc and,+
R6693 T22963 T22964 punct +,-
R6694 T22964 T22959 conj -,+
R6695 T22965 T22964 punct /,-
R6696 T22966 T22948 punct .,was
R6697 T22968 T22969 det The,numbers
R6698 T22969 T22970 nsubj numbers,indicates
R6699 T22971 T22969 prep on,numbers
R6700 T22972 T22973 det the,bars
R6701 T22973 T22971 pobj bars,on
R6702 T22974 T22975 det the,number
R6703 T22975 T22970 dobj number,indicates
R6704 T22976 T22975 prep of,number
R6705 T22977 T22976 pobj mice,of
R6706 T22978 T22977 acl analyzed,mice
R6707 T22979 T22970 punct .,indicates
R6708 T22981 T22982 punct *,**
R6709 T22983 T22981 punct ", ",*
R6710 T22984 T22985 advmod Significantly,different
R6711 T22985 T22981 amod different,*
R6712 T22986 T22985 prep from,different
R6713 T22987 T22988 punct +,+
R6714 T22988 T22986 punct +,from
R6715 T22989 T22988 punct /,+
R6716 T22990 T22991 punct (,0.001
R6717 T22991 T22985 parataxis 0.001,different
R6718 T22992 T22991 nsubj P,0.001
R6719 T22993 T22991 punct <,0.001
R6720 T22994 T22991 punct ),0.001
R6721 T22995 T22982 punct ;,**
R6722 T22996 T22982 punct ", ",**
R6723 T22997 T22998 advmod significantly,different
R6724 T22998 T22982 amod different,**
R6725 T22999 T22998 prep from,different
R6726 T23000 T23001 punct +,+
R6727 T23001 T22999 punct +,from
R6728 T23002 T23001 punct /,+
R6729 T23003 T23001 cc and,+
R6730 T23004 T23005 punct +,-
R6731 T23005 T23001 conj -,+
R6732 T23006 T23005 punct /,-
R6733 T23007 T23008 punct (,0.001
R6734 T23008 T22998 parataxis 0.001,different
R6735 T23009 T23008 nsubj P,0.001
R6736 T23010 T23008 punct <,0.001
R6737 T23011 T23008 punct ),0.001
R6738 T23012 T22982 punct .,**
R7209 T24775 T24774 prep of,Summary
R7210 T24776 T24777 amod additional,levels
R7211 T24777 T24775 pobj levels,of
R7212 T24778 T24777 compound blood,levels
R7213 T24779 T24780 compound amino,acid
R7214 T24780 T24777 compound acid,levels
R7215 T24781 T24777 prep in,levels
R7216 T24782 T24783 det the,model
R7217 T24783 T24781 pobj model,in
R7218 T24784 T24783 amod classic,model
R7219 T24785 T24783 compound MSUD,model
R7220 T24786 T24783 cc and,model
R7221 T24787 T24788 compound control,littermates
R7222 T24788 T24783 conj littermates,model
R7223 T24789 T24790 mark as,determined
R7224 T24790 T24777 advcl determined,levels
R7225 T24791 T24790 prep by,determined
R7226 T24792 T24793 amod tandem,spectrometry
R7227 T24793 T24791 pobj spectrometry,by
R7228 T24794 T24793 compound mass,spectrometry
R7229 T24795 T24774 punct .,Summary
R7230 T24797 T24798 det All,samples
R7231 T24798 T24799 nsubjpass samples,collected
R7232 T24800 T24799 auxpass were,collected
R7233 T24801 T24799 prep on,collected
R7234 T24802 T24803 det the,day
R7235 T24803 T24801 pobj day,on
R7236 T24804 T24803 prep of,day
R7237 T24805 T24804 pobj birth,of
R7238 T24806 T24799 punct .,collected
R7239 T24808 T24809 det All,values
R7240 T24809 T24810 nsubj values,are
R7241 T24811 T24810 attr mean,are
R7242 T24812 T24811 cc +,mean
R7243 T24813 T24812 punct /,+
R7244 T24814 T24812 punct -,+
R7245 T24815 T24811 conj SEM,mean
R7246 T24816 T24810 punct .,are
R3345 T12266 T12268 punct +,mice
R3347 T12268 T12264 pobj mice,in
R3348 T12269 T12270 advmod readily,detectable
R3349 T12270 T12256 acomp detectable,was
R3350 T12271 T12256 punct .,was
R3351 T12273 T12274 prep In,was
R3352 T12275 T12276 amod marked,contrast
R3353 T12276 T12273 pobj contrast,In
R3354 T12277 T12274 punct ", ",was
R3355 T12278 T12279 compound BCKDH,activity
R3356 T12279 T12274 nsubj activity,was
R3357 T12280 T12281 advmod completely,absent
R3358 T12281 T12274 acomp absent,was
R3359 T12282 T12274 prep in,was
R3360 T12283 T12284 punct -,-
R3361 T12284 T12286 punct -,livers
R3362 T12285 T12284 punct /,-
R3410 T12339 T12340 nsubjpass Immunohistochemistry,used
R3411 T12341 T12339 prep with,Immunohistochemistry
R3412 T12342 T12343 det an,antibody
R3413 T12343 T12341 pobj antibody,with
R3414 T12344 T12345 npadvmod E2,specific
R3415 T12345 T12343 amod specific,antibody
R3416 T12346 T12340 auxpass was,used
R3417 T12347 T12348 aux to,examine
R3418 T12348 T12340 advcl examine,used
R3419 T12349 T12350 compound E2,protein
R3721 T12671 T12667 pobj ratio,as
R3722 T12672 T12659 punct .,expressed
R3723 T12674 T12675 mark As,shown
R3724 T12675 T12676 advcl shown,was
R3725 T12677 T12675 prep in,shown
R3726 T12678 T12679 compound Figure,2C
R3727 T12679 T12677 pobj 2C,in
R3728 T12680 T12676 punct ", ",was
R3729 T12681 T12682 det this,ratio
R3730 T12682 T12676 nsubj ratio,was
R3731 T12683 T12682 prep in,ratio
R3732 T12684 T12685 punct -,-
R3733 T12685 T12687 punct -,pups
R3734 T12686 T12685 punct /,-
R3735 T12687 T12683 pobj pups,in
R3736 T12688 T12689 advmod more,6
R3737 T12689 T12691 quantmod 6,fold
R3738 T12690 T12689 quantmod than,6
R3739 T12691 T12693 advmod fold,higher
R3740 T12692 T12691 punct -,fold
R3741 T12693 T12676 acomp higher,was
R3742 T12694 T12693 prep than,higher
R3743 T12695 T12696 punct +,+
R3744 T12696 T12698 punct +,littermates
R3745 T12697 T12696 punct /,+
R3746 T12698 T12694 pobj littermates,than
R3747 T12699 T12696 cc or,+
R3774 T12729 T12730 prep In,lack
R3775 T12731 T12729 pobj summary,In
R3776 T12732 T12730 punct ", ",lack
R3777 T12733 T12734 compound E2,mice
R3778 T12734 T12730 nsubj mice,lack
R3779 T12735 T12734 compound knockout,mice
R3780 T12736 T12737 nmod BCKDH,activity
R3781 T12737 T12730 dobj activity,lack
R3782 T12738 T12737 amod enzymatic,activity
R3783 T12739 T12737 punct ", ",activity
R3784 T12740 T12741 compound E2,immunoreactivity
R3785 T12741 T12737 appos immunoreactivity,activity
R3786 T12742 T12730 punct ", ",lack
R3787 T12743 T12730 cc and,lack
R3788 T12744 T12730 conj have,lack
R3789 T12745 T12746 advmod markedly,elevated
R3790 T12746 T12747 amod elevated,levels
R3791 T12747 T12744 dobj levels,have
R3792 T12748 T12747 prep of,levels
R3793 T12749 T12748 pobj BCAA,of
R3794 T12750 T12744 prep in,have
R3795 T12751 T12752 det the,blood
R3796 T12752 T12750 pobj blood,in
R3797 T12753 T12752 cc and,blood
R3798 T12754 T12752 conj urine,blood
R3799 T12755 T12730 punct .,lack
R3800 T12757 T12758 det These,derangements
R3801 T12758 T12760 nsubj derangements,result
R3802 T12759 T12758 amod metabolic,derangements

craft-ca-core-ex-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T12205 4062-4066 NCBITaxon:10088 denotes mice
T12204 4050-4052 PR_EXT:000006300 denotes E2
T12203 4006-4012 NCBITaxon:9606 denotes humans
T12202 3935-3944 GO_EXT:fatality_or_lethality denotes lethality
T12201 3926-3934 PATO_UBERON_EXT:neonate_or_newborn denotes neonatal
T12200 3882-3891 GO:0008152 denotes metabolic
T12199 3869-3874 UBERON:0001088 denotes urine
T12198 3859-3864 UBERON:0000178 denotes blood
T12197 3847-3851 CHEBI:22918 denotes BCAA
T12196 3789-3791 PR_EXT:000006300 denotes E2
T12195 3769-3778 CHEBI_GO_SO_EXT:enzyme denotes enzymatic
T12194 3753-3757 NCBITaxon:10088 denotes mice
T12193 3741-3743 PR_EXT:000006300 denotes E2
T12078 0-2 PR_EXT:000006300 denotes E2
T12079 13-17 NCBITaxon:10088 denotes mice
T12080 72-76 SO_EXT:0000704 denotes gene
T12081 108-110 PR_EXT:000006300 denotes E2
T12082 111-115 SO_EXT:sequence_nullness denotes null
T12083 116-122 SO_EXT:0001023 denotes allele
T12084 156-161 UBERON:0002107 denotes liver
T12085 181-186 GO:0007567 denotes natal
T12086 193-198 NCBITaxon:10088 denotes mouse
T12087 199-203 NCBITaxon_UBERON_EXT:pup denotes pups
T12088 217-218 SO_EXT:normal_or_wild_type_or_present denotes +
T12089 219-220 SO_EXT:sequence_nullness_or_absence denotes -
T12090 224-225 SO_EXT:normal_or_wild_type_or_present denotes +
T12091 226-227 SO_EXT:sequence_nullness_or_absence denotes -
T12092 228-234 GO:0007618 denotes mating
T12093 287-288 SO_EXT:normal_or_wild_type_or_present denotes +
T12094 289-290 SO_EXT:normal_or_wild_type_or_present denotes +
T12095 291-295 NCBITaxon:10088 denotes mice
T12096 380-381 SO_EXT:sequence_nullness_or_absence denotes -
T12097 382-383 SO_EXT:sequence_nullness_or_absence denotes -
T12098 384-389 NCBITaxon:10088 denotes mouse
T12099 390-396 UBERON:0002107 denotes livers
T12100 428-429 SO_EXT:normal_or_wild_type_or_present denotes +
T12101 430-431 SO_EXT:sequence_nullness_or_absence denotes -
T12102 432-436 NCBITaxon:10088 denotes mice
T12103 482-483 SO_EXT:normal_or_wild_type_or_present denotes +
T12104 484-485 SO_EXT:normal_or_wild_type_or_present denotes +
T12105 528-529 SO_EXT:sequence_nullness_or_absence denotes -
T12106 530-531 SO_EXT:sequence_nullness_or_absence denotes -
T12107 532-536 NCBITaxon:10088 denotes mice
T12108 569-575 NCBITaxon:9606 denotes humans
T12148 2178-2187 GO:0006537 denotes glutamate
T12149 2193-2202 GO:0006542 denotes glutamine
T12150 2169-2176 CHEBI_SO_EXT:alanine denotes alanine
T12151 2178-2187 CHEBI_EXT:glutamate denotes glutamate
T12152 2193-2202 CHEBI_SO_EXT:glutamine denotes glutamine
T12153 2229-2247 GO:0009081 denotes metabolism of BCAA
T12154 2243-2247 CHEBI:22918 denotes BCAA
T12155 2256-2260 NCBITaxon:10088 denotes mice
T12156 2347-2352 UBERON:0000178 denotes blood
T12157 2367-2374 CHEBI_SO_EXT:alanine denotes alanine
T12158 2376-2385 CHEBI_EXT:glutamate denotes glutamate
T12159 2391-2400 CHEBI_SO_EXT:glutamine denotes glutamine
T12160 2447-2458 CHEBI_SO_EXT:amino_acid denotes amino acids
T12161 2473-2477 NCBITaxon:10088 denotes mice
T12162 2517-2518 SO_EXT:normal_or_wild_type_or_present denotes +
T12163 2519-2520 SO_EXT:normal_or_wild_type_or_present denotes +
T12164 2524-2525 SO_EXT:normal_or_wild_type_or_present denotes +
T12165 2526-2527 SO_EXT:sequence_nullness_or_absence denotes -
T12166 2528-2532 NCBITaxon:10088 denotes mice
T12167 2554-2565 CHEBI_SO_EXT:amino_acid denotes amino acids
T12168 2573-2574 SO_EXT:normal_or_wild_type_or_present denotes +
T12169 2575-2576 SO_EXT:sequence_nullness_or_absence denotes -
T12170 2577-2581 NCBITaxon:10088 denotes mice
T12171 2610-2611 SO_EXT:normal_or_wild_type_or_present denotes +
T12172 2612-2613 SO_EXT:normal_or_wild_type_or_present denotes +
T12173 2614-2618 NCBITaxon:10088 denotes mice
T12174 2670-2675 UBERON:0000178 denotes blood
T12175 2676-2683 CHEBI_SO_EXT:alanine denotes alanine
T12176 2709-2714 UBERON:0000178 denotes blood
T12177 2715-2719 CHEBI:22918 denotes BCAA
T12178 2825-2829 CHEBI:22918 denotes BCAA
T12179 2830-2837 CHEBI_SO_EXT:alanine denotes alanine
T12180 2914-2918 CHEBI:22918 denotes BCAA
T12181 2969-2979 CHEBI_SO_EXT:amino_acid denotes amino acid
T12182 2991-2995 CHEBI:22918 denotes BCAA
T12183 2996-3003 CHEBI_SO_EXT:alanine denotes alanine
T12184 3048-3049 SO_EXT:sequence_nullness_or_absence denotes -
T12185 3050-3051 SO_EXT:sequence_nullness_or_absence denotes -
T12186 3052-3056 NCBITaxon_UBERON_EXT:pup denotes pups
T12187 3090-3091 SO_EXT:normal_or_wild_type_or_present denotes +
T12188 3092-3093 SO_EXT:normal_or_wild_type_or_present denotes +
T12189 3097-3098 SO_EXT:normal_or_wild_type_or_present denotes +
T12190 3099-3100 SO_EXT:sequence_nullness_or_absence denotes -
T12191 3120-3125 UBERON:0000178 denotes blood
T12192 3126-3136 CHEBI_SO_EXT:amino_acid denotes amino acid
T22750 609-620 CHEBI_EXT:biochemical denotes Biochemical
T22751 658-664 NCBITaxon:39107 denotes murine
T22752 681-687 CHEBI_GO_SO_EXT:enzyme denotes enzyme
T22753 700-705 UBERON:0002107 denotes liver
T22754 709-716 PATO_UBERON_EXT:neonate_or_newborn denotes newborn
T22755 717-726 SO_EXT:wild_type_entity_or_quality denotes wild type
T22756 736-737 SO_EXT:normal_or_wild_type_or_present denotes +
T22757 738-739 SO_EXT:normal_or_wild_type_or_present denotes +
T22758 756-757 SO_EXT:normal_or_wild_type_or_present denotes +
T22759 758-759 SO_EXT:sequence_nullness_or_absence denotes -
T22760 778-779 SO_EXT:sequence_nullness_or_absence denotes -
T22761 780-781 SO_EXT:sequence_nullness_or_absence denotes -
T22762 792-796 NCBITaxon:10088 denotes mice
T22763 798-804 CHEBI_GO_SO_EXT:enzyme denotes Enzyme
T22764 843-844 SO_EXT:normal_or_wild_type_or_present denotes +
T22765 845-846 SO_EXT:sequence_nullness_or_absence denotes -
T22766 847-852 UBERON:0002107 denotes liver
T22767 865-866 SO_EXT:normal_or_wild_type_or_present denotes +
T22768 867-868 SO_EXT:normal_or_wild_type_or_present denotes +
T22769 910-911 SO_EXT:sequence_nullness_or_absence denotes -
T22770 912-913 SO_EXT:sequence_nullness_or_absence denotes -
T22771 914-919 UBERON:0002107 denotes liver
T22772 930-934 CHEBI:22918 denotes BCAA
T22773 953-958 UBERON:0000178 denotes blood
T22774 962-966 NCBITaxon:10088 denotes mice
T22775 974-978 CHEBI:22918 denotes BCAA
T22776 1000-1007 CHEBI_SO_EXT:leucine denotes leucine
T22777 1009-1019 CHEBI_SO_EXT:isoleucine denotes isoleucine
T22778 1025-1031 CHEBI_SO_EXT:valine denotes valine
T22779 1039-1043 CHEBI:22918 denotes BCAA
T22780 1062-1067 UBERON:0000178 denotes blood
T22781 1073-1074 SO_EXT:sequence_nullness_or_absence denotes -
T22782 1075-1076 SO_EXT:sequence_nullness_or_absence denotes -
T22783 1077-1081 NCBITaxon:10088 denotes mice
T22784 1122-1123 SO_EXT:normal_or_wild_type_or_present denotes +
T22785 1124-1125 SO_EXT:normal_or_wild_type_or_present denotes +
T22786 1130-1131 SO_EXT:normal_or_wild_type_or_present denotes +
T22787 1132-1133 SO_EXT:sequence_nullness_or_absence denotes -
T22788 1153-1157 CHEBI:22918 denotes BCAA
T22789 1161-1168 CHEBI_SO_EXT:alanine denotes alanine
T22790 1172-1177 UBERON:0000178 denotes blood
T22791 1181-1185 NCBITaxon:10088 denotes mice
T22792 1228-1229 SO_EXT:sequence_nullness_or_absence denotes -
T22793 1230-1231 SO_EXT:sequence_nullness_or_absence denotes -
T22794 1232-1236 NCBITaxon:10088 denotes mice
T22795 1249-1250 SO_EXT:normal_or_wild_type_or_present denotes +
T22796 1251-1252 SO_EXT:normal_or_wild_type_or_present denotes +
T22797 1257-1258 SO_EXT:normal_or_wild_type_or_present denotes +
T22798 1259-1260 SO_EXT:sequence_nullness_or_absence denotes -
T22799 1261-1265 NCBITaxon:10088 denotes mice
T22800 1315-1319 NCBITaxon:10088 denotes mice
T22801 1362-1363 SO_EXT:normal_or_wild_type_or_present denotes +
T22802 1364-1365 SO_EXT:normal_or_wild_type_or_present denotes +
T22803 1412-1413 SO_EXT:normal_or_wild_type_or_present denotes +
T22804 1414-1415 SO_EXT:normal_or_wild_type_or_present denotes +
T22805 1420-1421 SO_EXT:normal_or_wild_type_or_present denotes +
T22806 1422-1423 SO_EXT:sequence_nullness_or_absence denotes -
T24771 3252-3257 UBERON:0000178 denotes blood
T24772 3258-3268 CHEBI_SO_EXT:amino_acid denotes amino acid
T24773 3410-3415 GO:0007567 denotes birth
T12130 1799-1804 UBERON:0000178 denotes blood
T12131 1821-1826 UBERON:0001088 denotes urine
T12132 1854-1858 CHEBI:22918 denotes BCAA
T12109 1466-1468 PR_EXT:000006300 denotes E2
T12110 1478-1486 GO:0042571 denotes antibody
T12111 1507-1509 PR_EXT:000006300 denotes E2
T12112 1510-1517 CHEBI_PR_EXT:protein denotes protein
T12113 1525-1529 NCBITaxon:10088 denotes mice
T12114 1576-1578 PR_EXT:000006300 denotes E2
T12115 1579-1586 CHEBI_PR_EXT:protein denotes protein
T12116 1603-1608 UBERON:0002107 denotes liver
T12117 1613-1622 UBERON:0000922 denotes embryonic
T12118 1623-1634 CL:0000057 denotes fibroblasts
T12119 1637-1638 SO_EXT:normal_or_wild_type_or_present denotes +
T12120 1639-1640 SO_EXT:normal_or_wild_type_or_present denotes +
T12121 1641-1645 NCBITaxon:10088 denotes mice
T12122 1682-1684 PR_EXT:000006300 denotes E2
T12123 1685-1692 CHEBI_PR_EXT:protein denotes protein
T12124 1718-1725 UBERON:0000479 denotes tissues
T12125 1729-1730 SO_EXT:sequence_nullness_or_absence denotes -
T12126 1731-1732 SO_EXT:sequence_nullness_or_absence denotes -
T12127 1733-1737 NCBITaxon:10088 denotes mice
T12128 1750-1752 PR_EXT:000006300 denotes E2
T12129 1762-1766 NCBITaxon:10088 denotes mice
T12133 1867-1874 CHEBI_SO_EXT:leucine denotes leucine
T12134 1876-1886 CHEBI_SO_EXT:isoleucine denotes isoleucine
T12135 1892-1898 CHEBI_SO_EXT:valine denotes valine
T12136 1921-1922 SO_EXT:normal_or_wild_type_or_present denotes +
T12137 1923-1924 SO_EXT:normal_or_wild_type_or_present denotes +
T12138 1946-1957 CHEBI_SO_EXT:amino_acid denotes amino acids
T12139 2012-2016 CHEBI:22918 denotes BCAA
T12140 2053-2067 CHEBI_EXT:alloisoleucine denotes alloisoleucine
T12141 2099-2100 SO_EXT:sequence_nullness_or_absence denotes -
T12142 2101-2102 SO_EXT:sequence_nullness_or_absence denotes -
T12143 2108-2112 NCBITaxon:10088 denotes mice
T12144 2118-2136 GO:0009081 denotes metabolism of BCAA
T12145 2132-2136 CHEBI:22918 denotes BCAA
T12146 2156-2176 GO:0006523 denotes synthesis of alanine
T12147 2156-2168 _FRAGMENT denotes synthesis of
R3288 T12148 T12147 _lexicallyChainedTo glutamate,synthesis of
R3289 T12149 T12147 _lexicallyChainedTo glutamine,synthesis of

craft-ca-core-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T12076 4050-4052 PR:000006300 denotes E2
T12075 4006-4012 NCBITaxon:9606 denotes humans
T12074 3882-3891 GO:0008152 denotes metabolic
T12073 3869-3874 UBERON:0001088 denotes urine
T12072 3859-3864 UBERON:0000178 denotes blood
T12071 3847-3851 CHEBI:22918 denotes BCAA
T12070 3789-3791 PR:000006300 denotes E2
T12069 3753-3757 NCBITaxon:10088 denotes mice
T12068 3741-3743 PR:000006300 denotes E2
T12013 0-2 PR:000006300 denotes E2
T12014 13-17 NCBITaxon:10088 denotes mice
T12015 72-76 SO:0000704 denotes gene
T12016 108-110 PR:000006300 denotes E2
T12017 116-122 SO:0001023 denotes allele
T12018 156-161 UBERON:0002107 denotes liver
T12019 181-186 GO:0007567 denotes natal
T12020 193-198 NCBITaxon:10088 denotes mouse
T12021 228-234 GO:0007618 denotes mating
T12022 291-295 NCBITaxon:10088 denotes mice
T12023 384-389 NCBITaxon:10088 denotes mouse
T12024 390-396 UBERON:0002107 denotes livers
T12025 432-436 NCBITaxon:10088 denotes mice
T12026 532-536 NCBITaxon:10088 denotes mice
T12027 569-575 NCBITaxon:9606 denotes humans
T12028 1466-1468 PR:000006300 denotes E2
T12029 1478-1486 GO:0042571 denotes antibody
T12030 1507-1509 PR:000006300 denotes E2
T12031 1525-1529 NCBITaxon:10088 denotes mice
T12032 1576-1578 PR:000006300 denotes E2
T12033 1603-1608 UBERON:0002107 denotes liver
T12034 1613-1622 UBERON:0000922 denotes embryonic
T12035 1623-1634 CL:0000057 denotes fibroblasts
T12036 1641-1645 NCBITaxon:10088 denotes mice
T12037 1682-1684 PR:000006300 denotes E2
T12038 1718-1725 UBERON:0000479 denotes tissues
T12039 1733-1737 NCBITaxon:10088 denotes mice
T12040 1750-1752 PR:000006300 denotes E2
T12041 1762-1766 NCBITaxon:10088 denotes mice
T12042 1799-1804 UBERON:0000178 denotes blood
T12043 1821-1826 UBERON:0001088 denotes urine
T12044 1854-1858 CHEBI:22918 denotes BCAA
T12045 2012-2016 CHEBI:22918 denotes BCAA
T12046 2108-2112 NCBITaxon:10088 denotes mice
T12047 2118-2136 GO:0009081 denotes metabolism of BCAA
T12048 2132-2136 CHEBI:22918 denotes BCAA
T12049 2156-2176 GO:0006523 denotes synthesis of alanine
T12050 2156-2168 _FRAGMENT denotes synthesis of
T12051 2178-2187 GO:0006537 denotes glutamate
T12052 2193-2202 GO:0006542 denotes glutamine
T12053 2229-2247 GO:0009081 denotes metabolism of BCAA
T12054 2243-2247 CHEBI:22918 denotes BCAA
T12055 2256-2260 NCBITaxon:10088 denotes mice
T12056 2347-2352 UBERON:0000178 denotes blood
T12057 2473-2477 NCBITaxon:10088 denotes mice
T12058 2528-2532 NCBITaxon:10088 denotes mice
T12059 2577-2581 NCBITaxon:10088 denotes mice
T12060 2614-2618 NCBITaxon:10088 denotes mice
T12061 2670-2675 UBERON:0000178 denotes blood
T12062 2709-2714 UBERON:0000178 denotes blood
T12063 2715-2719 CHEBI:22918 denotes BCAA
T12064 2825-2829 CHEBI:22918 denotes BCAA
T12065 2914-2918 CHEBI:22918 denotes BCAA
T12066 2991-2995 CHEBI:22918 denotes BCAA
T12067 3120-3125 UBERON:0000178 denotes blood
T12077 4062-4066 NCBITaxon:10088 denotes mice
T22732 658-664 NCBITaxon:39107 denotes murine
T22733 700-705 UBERON:0002107 denotes liver
T22734 792-796 NCBITaxon:10088 denotes mice
T22735 847-852 UBERON:0002107 denotes liver
T22736 914-919 UBERON:0002107 denotes liver
T22737 930-934 CHEBI:22918 denotes BCAA
T22738 953-958 UBERON:0000178 denotes blood
T22739 962-966 NCBITaxon:10088 denotes mice
T22740 974-978 CHEBI:22918 denotes BCAA
T22741 1039-1043 CHEBI:22918 denotes BCAA
T22742 1062-1067 UBERON:0000178 denotes blood
T22743 1077-1081 NCBITaxon:10088 denotes mice
T22744 1153-1157 CHEBI:22918 denotes BCAA
T22745 1172-1177 UBERON:0000178 denotes blood
T22746 1181-1185 NCBITaxon:10088 denotes mice
T22747 1232-1236 NCBITaxon:10088 denotes mice
T22748 1261-1265 NCBITaxon:10088 denotes mice
T22749 1315-1319 NCBITaxon:10088 denotes mice
T24769 3252-3257 UBERON:0000178 denotes blood
T24770 3410-3415 GO:0007567 denotes birth
R3286 T12051 T12050 _lexicallyChainedTo glutamate,synthesis of
R3287 T12052 T12050 _lexicallyChainedTo glutamine,synthesis of